Prupe.6G010500_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000726
Physical Location & Seq
Forward (+)
791419 .. 792118
700 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G010500.1

Sequence Viewer

Length: 567 bp
ATGGCTAGAATTTCTCCTATCCTAGCTTTCCAACTCATAATTTCCTCCCTCTTCTCTTCCTTTGCCATGATCCCAGTTTCTGGGGAAAATCGTGGGTTTGTAGGAAGCATTGACCCTAAGCTTTTTGGACTGCAAGAAGAAAAGCTCACCCACATTCAATTATATTGGCATGACCTTCTTAATGGGCCTCAACCTTCTGCCATAGATGTGGTGCCTTCATCCACCAAGTCACCAACAAATTATGGGTTAGTGAGAATGTTTGATAATGCCCTAACCGAAGGACCTGAATTGAGCTCAGAATTGGTGGCAAGGGCTCAAGGGTTTTATGCATCTGCAGCACGAAAGGGGCTTAGCAAGTTGATGGTTCAGAACTTTGCTTTTGTCAAAGGTCAGTTTAATGGCAGCACCATAAATTTGATTGGAAGAAATTCAATTTTGAACAAGGTAAGGGAGCTGCCTGTGGTTGGTGGAAGCGGAGTTTTCAGGCTTGCTAGGGGTTATGCTTTAGCAACAACTCACACTGGTGATGCAACGGTGGAGTACAATATCTATGTGTTGCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

189

Amino Acids

20.5

Weight (kDa)

9.22

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 211
AccB7I CCANNNNNTGG 1 cut(s) 80
AciI CCGC 1 cut(s) 474
AclWI GGATC 1 cut(s) 64
AcsI RAATTY 3 cut(s) 9, 412, 427
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 2 cut(s) 80, 464
AgsI TTSAA 3 cut(s) 158, 432, 439
AleI CACNNNNGTG 1 cut(s) 522
AluBI AGCT 5 cut(s) 26, 121, 145, 294, 454
AluI AGCT 5 cut(s) 26, 121, 145, 294, 454
Alw21I GWGCWC 1 cut(s) 296
AlwI GGATC 1 cut(s) 64
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 3 cut(s) 335, 402, 454
ApoI RAATTY 3 cut(s) 9, 412, 427
Asp700I GAANNNNTTC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 185, 281
AsuHPI GGTGA 3 cut(s) 139, 222, 536
AvaII GGWCC 1 cut(s) 281
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 2 cut(s) 296, 316
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 3 cut(s) 347, 414, 441
BccI CCATC 1 cut(s) 355
BfaI CTAG 3 cut(s) 6, 23, 492
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 3 cut(s) 336, 403, 455
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 3 cut(s) 337, 404, 456
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 185, 281
BmiI GGNNCC 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 68
BmsI GCATC 2 cut(s) 338, 517
BmuI ACTGGG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 464
Bse1I ACTGG 2 cut(s) 74, 526
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 2 cut(s) 80, 464
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 74, 526
BseXI GCAGC 3 cut(s) 347, 414, 441
BshFI GGCC 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 80, 464
BsnI GGCC 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 296, 316
Bsp143I GATC 1 cut(s) 69
Bsp1720I GCTNAGC 1 cut(s) 350
BspACI CCGC 1 cut(s) 474
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 211
BsrI ACTGG 2 cut(s) 74, 526
BssMI GATC 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 2 cut(s) 56, 61
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 3 cut(s) 117, 295, 350
BstF5I GGATG 1 cut(s) 218
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 347, 414, 441
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuRI GGCC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 489
CaiI CAGNNNCTG 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 185, 281
Csp6I GTAC 1 cut(s) 541
CviAII CATG 2 cut(s) 67, 170
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 3 cut(s) 117, 295, 350
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 56, 61
EarI CTCTTC 2 cut(s) 56, 61
Ecl136II GAGCTC 1 cut(s) 294
Eco24I GRGCYC 2 cut(s) 296, 316
Eco47I GGWCC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 294
EcoICRI GAGCTC 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 2 cut(s) 296, 316
FaeI CATG 2 cut(s) 70, 173
FatI CATG 2 cut(s) 66, 169
Fnu4HI GCNGC 3 cut(s) 336, 403, 455
FokI GGATG 1 cut(s) 205
FriOI GRGCYC 2 cut(s) 296, 316
Fsp4HI GCNGC 3 cut(s) 336, 403, 455
FspBI CTAG 3 cut(s) 6, 23, 492
GluI GCNGC 3 cut(s) 336, 403, 455
HaeIII GGCC 1 cut(s) 187
Hin1II CATG 2 cut(s) 70, 173
HindIII AAGCTT 1 cut(s) 119
HphI GGTGA 3 cut(s) 139, 222, 536
Hpy188I TCNGA 2 cut(s) 298, 369
HpyAV CCTTC 4 cut(s) 185, 204, 225, 272
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4V TGCA 5 cut(s) 133, 329, 335, 530, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 3 cut(s) 117, 295, 350
Hsp92II CATG 2 cut(s) 70, 173
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 6 cut(s) 66, 87, 297, 469, 471, 507
Lsp1109I GCAGC 3 cut(s) 347, 414, 441
LweI GCATC 2 cut(s) 338, 517
MaeI CTAG 3 cut(s) 6, 23, 492
MaeIII GTNAC 1 cut(s) 228
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 4 cut(s) 43, 48, 149, 435
MhlI GDGCHC 2 cut(s) 296, 316
MluCI AATT 9 cut(s) 9, 39, 158, 238, 287, 299, 412, 427, 432
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 3 cut(s) 55, 59, 198
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 2 cut(s) 180, 396
MslI CAYNNNNRTG 2 cut(s) 206, 522
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeII GATC 1 cut(s) 69
NlaIII CATG 2 cut(s) 70, 173
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 228
NsiI ATGCAT 1 cut(s) 331
OliI CACNNNNGTG 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 427
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 3 cut(s) 337, 404, 456
PpuMI RGGWCCY 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 185, 281
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 2 cut(s) 206, 522
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 180, 396
SatI GCNGC 3 cut(s) 336, 403, 455
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 2 cut(s) 185, 281
SduI GDGCHC 2 cut(s) 296, 316
SfaNI GCATC 2 cut(s) 338, 517
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 206, 522
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 9 cut(s) 9, 39, 158, 238, 287, 299, 412, 427, 432
SsiI CCGC 1 cut(s) 474
SspMI CTAG 3 cut(s) 6, 23, 492
SstI GAGCTC 1 cut(s) 296
TaaI ACNGT 1 cut(s) 535
TasI AATT 9 cut(s) 9, 39, 158, 238, 287, 299, 412, 427, 432
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 2 cut(s) 180, 396
Tru9I TTAA 2 cut(s) 180, 396
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 228
TseI GCWGC 3 cut(s) 335, 402, 454
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 207
TspRI CASTG 1 cut(s) 526
Van91I CCANNNNNTGG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 3 cut(s) 9, 412, 427
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 3 cut(s) 6, 23, 492
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.