FvH4_3g44752

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000726
Physical Location & Seq
Reverse (-)
37265755 .. 37266753
999 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44752.t1

Sequence Viewer

Length: 675 bp
ATGATGAAGTCAAGCTGGATGGCGTGCTCCACCCGATCATTTCACTACATATATGGACACAACCTTCTCAGTTGGAGAAAGGTTCCAACAATGGCCAGAATACCTCCCATCTTTGCTACACAACTCATACTTCTCTCCCTTCTTTCTTCCTTTGCCCTCATTCTGGTCCGGGGAGAAGATGATCACGATTTCGTGAGAGCCATGGACCGCGATCTCTTGGGGCTCAAGAAAGAAAAGCTCAGTCACTTCCGGTTGTACTGGCATGACATTGTGGGCGGCAAAAACCCAAGCGCAGTCTCAGTGGTGAATCCACCCGGTAACTCATCAACGCTCTTCGGATTGGTAAGAATGATCGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTGCTAGGGAGGGCTCAAGGGTTCTATGCATCAGCTGCACAACAGGAGCTCGGGCTGTTGATGGCCATGAACTTTCATTTTACTCGGGGCAAATATAATGGAAGCACCATAACTGTGCTGGGGAGGAACCCGGCGCTCAACAAGGTGAGAGAGATGCCGGTGATCGGAGGAAGCGGGCTTTTCAGGTTTGCAAGAGGTTATGTGGAAGCAAGAACTCATACATTTAATATGAGCACCGGAGATGCCATTGTTGAGTACAATTGCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

225

Amino Acids

25.18

Weight (kDa)

9.69

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 81 - 221 1.4e-57 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 210
AciI CCGC 3 cut(s) 208, 276, 567
AcoI YGGCCR 2 cut(s) 93, 456
AfaI GTAC 2 cut(s) 257, 650
AfiI CCNNNNNNNGG 2 cut(s) 163, 557
AluBI AGCT 7 cut(s) 15, 238, 382, 387, 394, 428, 442
AluI AGCT 7 cut(s) 15, 238, 382, 387, 394, 428, 442
Alw21I GWGCWC 5 cut(s) 29, 389, 444, 629, 666
Alw26I GTCTC 1 cut(s) 301
Ama87I CYCGRG 2 cut(s) 443, 477
AoxI GGCC 2 cut(s) 93, 456
ApeKI GCWGC 2 cut(s) 394, 428
AspLEI GCGC 2 cut(s) 293, 529
AspS9I GGNCC 2 cut(s) 166, 205
AsuC2I CCSGG 3 cut(s) 170, 315, 524
AsuHPI GGTGA 4 cut(s) 316, 358, 550, 565
AvaI CYCGRG 2 cut(s) 443, 477
AvaII GGWCC 2 cut(s) 166, 205
BalI TGGCCA 2 cut(s) 95, 458
BanII GRGCYC 4 cut(s) 225, 389, 409, 444
Bbv12I GWGCWC 5 cut(s) 29, 389, 444, 629, 666
BbvI GCAGC 2 cut(s) 381, 415
BccI CCATC 3 cut(s) 13, 116, 448
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 3 cut(s) 170, 315, 524
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 398
BfoI RGCGCY 1 cut(s) 530
BisI GCNGC 3 cut(s) 277, 395, 429
BlpI GCTNAGC 1 cut(s) 383
BlsI GCNGC 3 cut(s) 278, 396, 430
Bme1390I CCNGG 3 cut(s) 170, 315, 524
Bme18I GGWCC 2 cut(s) 166, 205
BmeT110I CYCGRG 2 cut(s) 443, 477
BmgT120I GGNCC 2 cut(s) 166, 205
BmiI GGNNCC 2 cut(s) 84, 521
BmrFI CCNGG 3 cut(s) 170, 315, 524
BmsI GCATC 3 cut(s) 431, 537, 625
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu1102I GCTNAGC 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 209, 393
BpuMI CCSGG 3 cut(s) 170, 315, 524
BsaJI CCNNGG 2 cut(s) 169, 201
BsaWI WCCGGW 2 cut(s) 249, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 557
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 169, 201
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 163, 557
BseMII CTCAG 4 cut(s) 82, 253, 312, 374
BseNI ACTGG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 381, 415
BseYI CCCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 95, 458
BsiHKAI GWGCWC 5 cut(s) 29, 389, 444, 629, 666
BsiHKCI CYCGRG 2 cut(s) 443, 477
BsiSI CCGG 7 cut(s) 169, 250, 315, 377, 524, 551, 630
BslI CCNNNNNNNGG 2 cut(s) 163, 557
BsmAI GTCTC 1 cut(s) 301
BsnI GGCC 2 cut(s) 95, 458
BsoBI CYCGRG 2 cut(s) 443, 477
Bsp1286I GDGCHC 7 cut(s) 29, 225, 389, 409, 444, 629, 666
Bsp143I GATC 5 cut(s) 35, 181, 211, 351, 555
Bsp1720I GCTNAGC 1 cut(s) 383
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 3 cut(s) 208, 276, 567
BspANI GGCC 2 cut(s) 95, 458
BspCNI CTCAG 4 cut(s) 81, 252, 311, 375
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 84, 521
BspQI GCTCTTC 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 169, 201
BssMI GATC 5 cut(s) 35, 181, 211, 351, 555
BssT1I CCWWGG 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 25, 569
BstDEI CTNAG 4 cut(s) 68, 239, 298, 383
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 24
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 2 cut(s) 293, 529
BstKTI GATC 5 cut(s) 38, 184, 214, 354, 558
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 5 cut(s) 35, 181, 211, 351, 555
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 168, 313, 522
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 381, 415
BsuRI GGCC 2 cut(s) 95, 458
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 25, 569
CfoI GCGC 2 cut(s) 293, 529
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 166, 205
Csp6I GTAC 2 cut(s) 256, 649
CviAII CATG 3 cut(s) 202, 263, 460
CviQI GTAC 2 cut(s) 256, 649
DdeI CTNAG 4 cut(s) 68, 239, 298, 383
DpnI GATC 5 cut(s) 37, 183, 213, 353, 557
DpnII GATC 5 cut(s) 35, 181, 211, 351, 555
EaeI YGGCCR 2 cut(s) 93, 456
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Ecl136II GAGCTC 2 cut(s) 387, 442
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 4 cut(s) 225, 389, 409, 444
Eco47I GGWCC 2 cut(s) 166, 205
Eco53kI GAGCTC 2 cut(s) 387, 442
Eco88I CYCGRG 2 cut(s) 443, 477
EcoICRI GAGCTC 2 cut(s) 387, 442
EcoT14I CCWWGG 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 4 cut(s) 225, 389, 409, 444
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 3 cut(s) 205, 266, 463
FatI CATG 3 cut(s) 201, 262, 459
FauI CCCGC 1 cut(s) 560
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 277, 395, 429
FokI GGATG 1 cut(s) 31
FriOI GRGCYC 4 cut(s) 225, 389, 409, 444
Fsp4HI GCNGC 3 cut(s) 277, 395, 429
FspBI CTAG 1 cut(s) 398
GlaI GCGC 2 cut(s) 292, 528
GluI GCNGC 3 cut(s) 277, 395, 429
GsaI CCCAGC 1 cut(s) 515
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 2 cut(s) 95, 458
HapII CCGG 7 cut(s) 169, 250, 315, 377, 524, 551, 630
HhaI GCGC 2 cut(s) 293, 529
Hin1II CATG 3 cut(s) 205, 266, 463
Hin6I GCGC 2 cut(s) 291, 527
HinP1I GCGC 2 cut(s) 291, 527
HinfI GANTC 1 cut(s) 307
HpaII CCGG 7 cut(s) 169, 250, 315, 377, 524, 551, 630
HphI GGTGA 4 cut(s) 316, 358, 550, 565
Hpy188I TCNGA 2 cut(s) 338, 560
Hpy188III TCNNGA 3 cut(s) 185, 193, 226
HpyAV CCTTC 2 cut(s) 74, 149
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4V TGCA 3 cut(s) 422, 431, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 4 cut(s) 68, 239, 298, 383
Hsp92II CATG 3 cut(s) 205, 266, 463
HspAI GCGC 2 cut(s) 291, 527
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 5 cut(s) 35, 181, 211, 351, 555
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 4 cut(s) 32, 379, 439, 669
Lsp1109I GCAGC 2 cut(s) 381, 415
LweI GCATC 3 cut(s) 431, 537, 625
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 2 cut(s) 242, 317
MalI GATC 5 cut(s) 37, 183, 213, 353, 557
MboI GATC 5 cut(s) 35, 181, 211, 351, 555
MboII GAAGA 3 cut(s) 138, 188, 325
MfeI CAATTG 1 cut(s) 652
MhlI GDGCHC 7 cut(s) 29, 225, 389, 409, 444, 629, 666
MlsI TGGCCA 2 cut(s) 95, 458
MluCI AATT 1 cut(s) 652
MluNI TGGCCA 2 cut(s) 95, 458
MmeI TCCRAC 2 cut(s) 53, 110
MnlI CCTC 7 cut(s) 114, 167, 374, 396, 510, 554, 581
Mox20I TGGCCA 2 cut(s) 95, 458
Mph1103I ATGCAT 1 cut(s) 424
MscI TGGCCA 2 cut(s) 95, 458
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 2 cut(s) 95, 458
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 7 cut(s) 169, 250, 315, 377, 524, 551, 630
MspR9I CCNGG 3 cut(s) 170, 315, 524
MunI CAATTG 1 cut(s) 652
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 428
NciI CCSGG 3 cut(s) 170, 315, 524
NcoI CCATGG 1 cut(s) 201
NdeII GATC 5 cut(s) 35, 181, 211, 351, 555
NlaIII CATG 3 cut(s) 205, 266, 463
NlaIV GGNNCC 2 cut(s) 84, 521
NmuCI GTSAC 1 cut(s) 242
NsiI ATGCAT 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 3 cut(s) 278, 396, 430
Psp124BI GAGCTC 2 cut(s) 389, 444
PspFI CCCAGC 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 84, 521
PspPI GGNCC 2 cut(s) 166, 205
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 2 cut(s) 257, 650
RsaNI GTAC 2 cut(s) 256, 649
RseI CAYNNNNRTG 1 cut(s) 506
SacI GAGCTC 2 cut(s) 389, 444
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 3 cut(s) 277, 395, 429
Sau3AI GATC 5 cut(s) 35, 181, 211, 351, 555
Sau96I GGNCC 2 cut(s) 166, 205
ScrFI CCNGG 3 cut(s) 170, 315, 524
SduI GDGCHC 7 cut(s) 29, 225, 389, 409, 444, 629, 666
SfaNI GCATC 3 cut(s) 431, 537, 625
SinI GGWCC 2 cut(s) 166, 205
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 2 cut(s) 224, 408
SmoI CTYRAG 2 cut(s) 224, 408
Sse9I AATT 1 cut(s) 652
SsiI CCGC 3 cut(s) 208, 276, 567
SspMI CTAG 1 cut(s) 398
SstI GAGCTC 2 cut(s) 389, 444
StyD4I CCNGG 3 cut(s) 168, 313, 522
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 508
TaqI TCGA 2 cut(s) 354, 389
TasI AATT 1 cut(s) 652
TatI WGTACW 2 cut(s) 255, 648
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 2 cut(s) 394, 428
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 20, 458, 476
TspRI CASTG 1 cut(s) 306
VpaK11BI GGWCC 2 cut(s) 166, 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XspI CTAG 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.