Rorug05G0476500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000726
Physical Location & Seq
Forward (+)
65558546 .. 65564180
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0476500.1

Sequence Viewer

Length: 948 bp
ATGGGAGAGGAGGTGAGGATGGGCGAGTACGAAGGAGGCGGCAGCGGCGGCGACGGCGACGAGGAGAGGGTTTCGGAGTGGGAGGCCGGGCTTCCGAACACCGATGATCTGACTCCGCTGTCTCAGCCGTTGATTCCGATGGAGCTGGCGTCGGCGTTCAGTATCTCGCCGGAGCCGTGCCGCACTGTGGTGGACGTCAACCGCGCGTCGCAGAAGACGGTGTCGACGCTGCGTGGGGGAGCTCACTCGCAGGCGTATTCGTCGAACTACAAGTCGTTCGAGGAGAACAACAACCGCAGCGACGAGCCGATGGTGGTGGAGCTGGACGAGACGGAGCCGTACGGCGGCGGCGGGGAAGGATCCGATTCGAGGAAGTCGAGGAGGGTTGATAGCCCCGAGGAGGCGGATTCGGCGCTCCGGATCGAGAATTCCTCGGGCGGCGGCGGAGACGACCCGTCGGGTCGGGCGGTCAAGCGGCCGAGGCTGGTGTGGACCCCGCAGCTGCACAAGCGGTTCGTGGACGTGGTGGCCCACCTGGGGATCAAGAACGCGGTTCCCAAGACGATTATGCAGCTGATGAACGTGGAAGGATTGACGCGTGAGAATGTGGCCAGCCACTTGCAGAAGTACCGACTCTACTTGAAGAGGATGCAGGGCTTGTCCAACGAGGGCCCCTCCTCGTCCGACCCGCTTTTCGCCTCGACTCCGGTGCCGCAGAGTCTGCACGAGTCGGGTGGGAGCGGGTCGGGTCACAGACAGGGGCACATGCCCATCTCGATCCCCATGCCTTACCCGCCGGGCGCCATGATGGCTATGCCTACTAGTTACATGGGGATGTCCGGCGGGTATCATGGGTTCGAGTCGCATCCTTCGTATAACATGATGCAGCAGCAGAAGTATGGTTCCATGGTGCCATACCCACCACATGTTGCTCCTAATGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

315

Amino Acids

34.17

Weight (kDa)

5.41

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 162 - 211 2.2e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AatII GACGTC 1 cut(s) 198
AccB1I GGYRCC 3 cut(s) 709, 800, 910
AccBSI CCGCTC 1 cut(s) 741
AccI GTMKAC 1 cut(s) 224
AccII CGCG 4 cut(s) 204, 206, 551, 598
AccIII TCCGGA 1 cut(s) 417
AclWI GGATC 5 cut(s) 354, 367, 428, 548, 772
AcoI YGGCCR 2 cut(s) 476, 609
AcsI RAATTY 1 cut(s) 427
AcyI GRCGYC 3 cut(s) 149, 195, 801
AfaI GTAC 3 cut(s) 29, 341, 629
AfiI CCNNNNNNNGG 5 cut(s) 187, 344, 369, 400, 537
AflIII ACRYGT 2 cut(s) 596, 925
AgsI TTSAA 1 cut(s) 643
AhdI GACNNNNNGTC 1 cut(s) 454
AhlI ACTAGT 1 cut(s) 821
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 534
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 5 cut(s) 145, 242, 322, 502, 574
AluI AGCT 5 cut(s) 145, 242, 322, 502, 574
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 126, 323, 441
AlwI GGATC 5 cut(s) 354, 367, 428, 548, 772
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 2 cut(s) 395, 433
Aor13HI TCCGGA 1 cut(s) 417
AoxI GGCC 5 cut(s) 84, 476, 528, 609, 670
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 8 cut(s) 42, 229, 297, 499, 502, 571, 886, 889
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 4 cut(s) 257, 289, 677, 709
AspLEI GCGC 3 cut(s) 206, 415, 803
AspS9I GGNCC 4 cut(s) 492, 529, 670, 671
AsuC2I CCSGG 2 cut(s) 88, 798
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 2 cut(s) 395, 433
AvaII GGWCC 1 cut(s) 492
BaeGI GKGCMC 2 cut(s) 674, 765
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 359
BanI GGYRCC 3 cut(s) 709, 800, 910
BanII GRGCYC 2 cut(s) 244, 674
BauI CACGAG 1 cut(s) 725
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 8 cut(s) 54, 216, 309, 489, 511, 583, 898, 901
BccI CCATC 5 cut(s) 13, 133, 304, 779, 802
BceAI ACGGC 5 cut(s) 70, 112, 160, 322, 358
BcgI CGANNNNNNTGC 4 cut(s) 691, 725, 766, 800
BciT130I CCWGG 1 cut(s) 536
BcnI CCSGG 2 cut(s) 88, 798
BcoDI GTCTC 3 cut(s) 126, 323, 441
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 1 cut(s) 822
BfoI RGCGCY 2 cut(s) 416, 804
BglI GCCNNNNNGGC 1 cut(s) 809
Bme1390I CCNGG 3 cut(s) 88, 536, 798
Bme18I GGWCC 1 cut(s) 492
BmeRI GACNNNNNGTC 1 cut(s) 454
BmeT110I CYCGRG 2 cut(s) 395, 433
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 4 cut(s) 492, 529, 670, 671
BmrFI CCNGG 3 cut(s) 88, 536, 798
BmsI GCATC 3 cut(s) 639, 873, 874
BpiI GAAGAC 1 cut(s) 221
BplI GAGNNNNNCTC 4 cut(s) 416, 448, 659, 691
BpuMI CCSGG 2 cut(s) 88, 798
BsaHI GRCGYC 3 cut(s) 149, 195, 801
BsaJI CCNNGG 5 cut(s) 396, 432, 479, 535, 906
BsaWI WCCGGW 2 cut(s) 417, 706
BsaXI ACNNNNNCTCC 3 cut(s) 27, 134, 164
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 344, 369, 400, 537
BseAI TCCGGA 1 cut(s) 417
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 5 cut(s) 396, 432, 479, 535, 906
BseGI GGATG 4 cut(s) 24, 654, 840, 865
BseLI CCNNNNNNNGG 5 cut(s) 187, 344, 369, 400, 537
BseMII CTCAG 1 cut(s) 137
BseRI GAGGAG 6 cut(s) 23, 77, 296, 394, 413, 667
BseSI GKGCMC 2 cut(s) 674, 765
BseX3I CGGCCG 1 cut(s) 476
BseXI GCAGC 8 cut(s) 54, 216, 309, 489, 511, 583, 898, 901
BsgI GTGCAG 2 cut(s) 488, 707
Bsh1236I CGCG 4 cut(s) 204, 206, 551, 598
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 5 cut(s) 86, 478, 530, 611, 672
BshNI GGYRCC 3 cut(s) 709, 800, 910
BsiEI CGRYCG 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 244
BsiHKCI CYCGRG 2 cut(s) 395, 433
BsiSI CCGG 6 cut(s) 87, 170, 418, 707, 797, 840
BsiWI CGTACG 1 cut(s) 339
BslI CCNNNNNNNGG 5 cut(s) 187, 344, 369, 400, 537
BsmAI GTCTC 3 cut(s) 126, 323, 441
BsmBI CGTCTC 2 cut(s) 323, 441
BsnI GGCC 5 cut(s) 86, 478, 530, 611, 672
BsoBI CYCGRG 2 cut(s) 395, 433
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 244, 674, 765
Bsp13I TCCGGA 1 cut(s) 417
Bsp143I GATC 5 cut(s) 106, 359, 420, 540, 777
Bsp19I CCATGG 1 cut(s) 906
BspANI GGCC 5 cut(s) 86, 478, 530, 611, 672
BspCNI CTCAG 1 cut(s) 136
BspEI TCCGGA 1 cut(s) 417
BspFNI CGCG 4 cut(s) 204, 206, 551, 598
BspPI GGATC 5 cut(s) 354, 367, 428, 548, 772
BspT107I GGYRCC 3 cut(s) 709, 800, 910
BsrBI CCGCTC 1 cut(s) 741
BssECI CCNNGG 5 cut(s) 396, 432, 479, 535, 906
BssMI GATC 5 cut(s) 106, 359, 420, 540, 777
BssNI GRCGYC 3 cut(s) 149, 195, 801
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 187, 220
Bst6I CTCTTC 1 cut(s) 638
BstACI GRCGYC 3 cut(s) 149, 195, 801
BstAPI GCANNNNNTGC 1 cut(s) 721
BstC8I GCNNGC 3 cut(s) 147, 252, 613
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 1 cut(s) 906
BstF5I GGATG 4 cut(s) 24, 654, 840, 865
BstFNI CGCG 4 cut(s) 204, 206, 551, 598
BstH2I RGCGCY 2 cut(s) 416, 804
BstHHI GCGC 3 cut(s) 206, 415, 803
BstKTI GATC 5 cut(s) 109, 362, 423, 543, 780
BstMAI GTCTC 3 cut(s) 126, 323, 441
BstMBI GATC 5 cut(s) 106, 359, 420, 540, 777
BstMCI CGRYCG 1 cut(s) 479
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 2 cut(s) 769, 929
BstSCI CCNGG 3 cut(s) 86, 534, 796
BstSLI GKGCMC 2 cut(s) 674, 765
BstUI CGCG 4 cut(s) 204, 206, 551, 598
BstV1I GCAGC 8 cut(s) 54, 216, 309, 489, 511, 583, 898, 901
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BstZI CGGCCG 1 cut(s) 476
BsuRI GGCC 5 cut(s) 86, 478, 530, 611, 672
BtgI CCRYGG 1 cut(s) 906
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 4 cut(s) 24, 654, 840, 865
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 147, 252, 613
CaiI CAGNNNCTG 1 cut(s) 721
CfoI GCGC 3 cut(s) 206, 415, 803
Cfr13I GGNCC 4 cut(s) 492, 529, 670, 671
CseI GACGC 4 cut(s) 138, 195, 235, 604
Csp6I GTAC 3 cut(s) 28, 340, 628
CviAII CATG 8 cut(s) 766, 784, 805, 829, 851, 880, 907, 926
CviQI GTAC 3 cut(s) 28, 340, 628
DdeI CTNAG 1 cut(s) 123
DinI GGCGCC 1 cut(s) 802
DpnI GATC 5 cut(s) 108, 361, 422, 542, 779
DpnII GATC 5 cut(s) 106, 359, 420, 540, 777
DrdI GACNNNNNNGTC 1 cut(s) 118
DriI GACNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 476, 609
EagI CGGCCG 1 cut(s) 476
Eam1104I CTCTTC 1 cut(s) 638
Eam1105I GACNNNNNGTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 638
EciI GGCGGA 2 cut(s) 419, 459
Ecl136II GAGCTC 1 cut(s) 242
EclXI CGGCCG 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 906
Eco24I GRGCYC 2 cut(s) 244, 674
Eco47I GGWCC 1 cut(s) 492
Eco52I CGGCCG 1 cut(s) 476
Eco53kI GAGCTC 1 cut(s) 242
Eco88I CYCGRG 2 cut(s) 395, 433
EcoICRI GAGCTC 1 cut(s) 242
EcoO109I RGGNCCY 2 cut(s) 670, 671
EcoRI GAATTC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 244, 674
EgeI GGCGCC 1 cut(s) 802
EheI GGCGCC 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 906
Esp3I CGTCTC 2 cut(s) 323, 441
FaeI CATG 8 cut(s) 769, 787, 808, 832, 854, 883, 910, 929
FatI CATG 8 cut(s) 765, 783, 804, 828, 850, 879, 906, 925
FauI CCCGC 6 cut(s) 344, 504, 696, 734, 801, 836
FblI GTMKAC 1 cut(s) 224
FokI GGATG 4 cut(s) 31, 661, 847, 852
FriOI GRGCYC 2 cut(s) 244, 674
FspBI CTAG 1 cut(s) 822
GlaI GCGC 3 cut(s) 205, 414, 802
HaeII RGCGCY 2 cut(s) 416, 804
HaeIII GGCC 5 cut(s) 86, 478, 530, 611, 672
HapII CCGG 6 cut(s) 87, 170, 418, 707, 797, 840
HgaI GACGC 4 cut(s) 138, 195, 235, 604
HhaI GCGC 3 cut(s) 206, 415, 803
Hin1I GRCGYC 3 cut(s) 149, 195, 801
Hin1II CATG 8 cut(s) 769, 787, 808, 832, 854, 883, 910, 929
Hin6I GCGC 3 cut(s) 204, 413, 801
HinP1I GCGC 3 cut(s) 204, 413, 801
HincII GTYRAC 2 cut(s) 199, 225
HindII GTYRAC 2 cut(s) 199, 225
HinfI GANTC 9 cut(s) 112, 133, 365, 407, 633, 703, 718, 728, 860
HpaII CCGG 6 cut(s) 87, 170, 418, 707, 797, 840
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 5 cut(s) 193, 199, 225, 492, 520
Hpy188I TCNGA 6 cut(s) 76, 96, 111, 138, 364, 685
Hpy188III TCNNGA 4 cut(s) 418, 424, 544, 775
Hpy8I GTNNAC 5 cut(s) 193, 199, 225, 492, 520
Hpy99I CGWCG 8 cut(s) 56, 62, 154, 211, 229, 265, 305, 460
HpyAV CCTTC 4 cut(s) 26, 350, 581, 879
HpyCH4III ACNGT 2 cut(s) 187, 220
HpyCH4IV ACGT 3 cut(s) 195, 522, 582
HpyCH4V TGCA 6 cut(s) 505, 571, 622, 652, 724, 886
HpyF3I CTNAG 1 cut(s) 123
HpySE526I ACGT 3 cut(s) 195, 522, 582
Hsp92I GRCGYC 3 cut(s) 149, 195, 801
Hsp92II CATG 8 cut(s) 769, 787, 808, 832, 854, 883, 910, 929
HspAI GCGC 3 cut(s) 204, 413, 801
KasI GGCGCC 1 cut(s) 800
Kpn2I TCCGGA 1 cut(s) 417
Kzo9I GATC 5 cut(s) 106, 359, 420, 540, 777
LmnI GCTCC 8 cut(s) 142, 172, 239, 319, 334, 420, 738, 937
Lsp1109I GCAGC 8 cut(s) 54, 216, 309, 489, 511, 583, 898, 901
LweI GCATC 3 cut(s) 639, 873, 874
MaeI CTAG 1 cut(s) 822
MaeII ACGT 3 cut(s) 195, 522, 582
MaeIII GTNAC 2 cut(s) 749, 824
MalI GATC 5 cut(s) 108, 361, 422, 542, 779
MbiI CCGCTC 1 cut(s) 741
MboI GATC 5 cut(s) 106, 359, 420, 540, 777
MboII GAAGA 2 cut(s) 226, 655
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 3 cut(s) 244, 674, 765
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 1 cut(s) 427
MluI ACGCGT 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 611
Mly113I GGCGCC 1 cut(s) 801
MlyI GAGTC 6 cut(s) 106, 627, 697, 727, 737, 869
MmeI TCCRAC 2 cut(s) 687, 708
Mox20I TGGCCA 1 cut(s) 611
MroI TCCGGA 1 cut(s) 417
MscI TGGCCA 1 cut(s) 611
MslI CAYNNNNRTG 2 cut(s) 188, 833
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 4 cut(s) 45, 118, 502, 574
MspI CCGG 6 cut(s) 87, 170, 418, 707, 797, 840
MspR9I CCNGG 3 cut(s) 88, 536, 798
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 4 cut(s) 204, 206, 551, 598
NarI GGCGCC 1 cut(s) 801
NciI CCSGG 2 cut(s) 88, 798
NcoI CCATGG 1 cut(s) 906
NdeII GATC 5 cut(s) 106, 359, 420, 540, 777
NlaIII CATG 8 cut(s) 769, 787, 808, 832, 854, 883, 910, 929
NmeAIII GCCGAG 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 749
NspI RCATGY 2 cut(s) 769, 929
OliI CACNNNNGTG 1 cut(s) 188
PciI ACATGT 1 cut(s) 925
PcsI WCGNNNNNNNCGW 3 cut(s) 173, 374, 869
PfeI GAWTC 3 cut(s) 133, 365, 407
Pfl23II CGTACG 1 cut(s) 339
PflFI GACNNNGTC 1 cut(s) 220
PleI GAGTC 6 cut(s) 106, 627, 697, 726, 736, 868
PluTI GGCGCC 1 cut(s) 804
PpsI GAGTC 6 cut(s) 106, 627, 697, 726, 736, 868
PscI ACATGT 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspLI CGTACG 1 cut(s) 339
PspOMI GGGCCC 1 cut(s) 670
PspPI GGNCC 4 cut(s) 492, 529, 670, 671
PsrI GAACNNNNNNTAC 2 cut(s) 839, 871
PstNI CAGNNNCTG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 359
PsyI GACNNNGTC 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 502, 574
RsaI GTAC 3 cut(s) 29, 341, 629
RsaNI GTAC 3 cut(s) 28, 340, 628
RseI CAYNNNNRTG 2 cut(s) 188, 833
SacI GAGCTC 1 cut(s) 244
SalI GTCGAC 1 cut(s) 223
Sau3AI GATC 5 cut(s) 106, 359, 420, 540, 777
Sau96I GGNCC 4 cut(s) 492, 529, 670, 671
SchI GAGTC 6 cut(s) 106, 627, 697, 727, 737, 869
ScrFI CCNGG 3 cut(s) 88, 536, 798
SduI GDGCHC 3 cut(s) 244, 674, 765
SfaNI GCATC 3 cut(s) 639, 873, 874
SfoI GGCGCC 1 cut(s) 802
SinI GGWCC 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 188, 833
SpeI ACTAGT 1 cut(s) 821
Sse9I AATT 1 cut(s) 427
SspDI GGCGCC 1 cut(s) 800
SspMI CTAG 1 cut(s) 822
SstI GAGCTC 1 cut(s) 244
StyD4I CCNGG 3 cut(s) 86, 534, 796
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 2 cut(s) 187, 220
TaiI ACGT 3 cut(s) 198, 525, 585
TaqI TCGA 9 cut(s) 224, 263, 279, 368, 377, 423, 701, 776, 858
TasI AATT 1 cut(s) 427
TfiI GAWTC 3 cut(s) 133, 365, 407
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 8 cut(s) 42, 229, 297, 499, 502, 571, 886, 889
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 1 cut(s) 593
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 190
Tth111I GACNNNGTC 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 492
XapI RAATTY 1 cut(s) 427
XceI RCATGY 2 cut(s) 769, 929
XmiI GTMKAC 1 cut(s) 224
XspI CTAG 1 cut(s) 822
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.