RLG00000036940

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000726
Physical Location & Seq
Reverse (-)
86708702 .. 86727461
18760 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036940

Sequence Viewer

Length: 585 bp
ATGGCCAGAATACCTTCCATCGTTGCTACACAGTTCATAATTCTCTCCCTTCTTTCTTCCTTTGCCCTAATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAGAGCCATGGACCGCGATCTCTTGGGGCTCAAGGAAGAAAAGCTTAGCCACTTCAAGTTGTACTGGCATGACATTGTGAGCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGTTAACTCATCAACGCTCTTCGGATTCGTGAGTATGATCGACGATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTGCTGGGGAGGGCTCAAGGGTTCTACGCATCAGCTTCACAACAGGAGGTCGGGCTGTTGATGGCTATGAACTTTCATTTTATTCAGGGCAAGTACAACGGGAGCACCATAACCGTGCTGGGGAGGAACCCAGTGTTCAACAAGGTGAGGGAGATGCCGGTGATCGGAGGAAGTGGGCTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCATACATTCACTGCGAGCACCGGAGATGCCATCGTTGAGTACAATGCCTACGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.48

Weight (kDa)

7.02

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 191 1.4e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 186
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 3 cut(s) 167, 398, 560
AfiI CCNNNNNNNGG 3 cut(s) 73, 423, 467
AgsI TTSAA 3 cut(s) 160, 442, 509
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
AluI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
Alw21I GWGCWC 4 cut(s) 299, 410, 539, 576
AlwI GGATC 1 cut(s) 263
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 76, 115
AsuHPI GGTGA 5 cut(s) 213, 226, 268, 460, 475
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 4 cut(s) 299, 410, 539, 576
BbvI GCAGC 1 cut(s) 291
BccI CCATC 3 cut(s) 26, 358, 557
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 91
BisI GCNGC 2 cut(s) 187, 305
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 2 cut(s) 188, 306
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 3 cut(s) 341, 447, 535
BmuI ACTGGG 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 2 cut(s) 119, 303
BsaAI YACGTR 1 cut(s) 571
BsaJI CCNNGG 2 cut(s) 79, 111
BsaWI WCCGGW 1 cut(s) 539
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 423, 467
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 173, 434
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 3 cut(s) 73, 423, 467
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 173, 434
BseXI GCAGC 1 cut(s) 291
BseYI CCCAGC 2 cut(s) 307, 421
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 4 cut(s) 299, 410, 539, 576
BsiSI CCGG 3 cut(s) 287, 461, 540
BslI CCNNNNNNNGG 3 cut(s) 73, 423, 467
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
Bsp143I GATC 5 cut(s) 91, 121, 261, 268, 465
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 118, 186
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 431
BspPI GGATC 1 cut(s) 263
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 173, 434
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 5 cut(s) 91, 121, 261, 268, 465
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 33, 418
Bst6I CTCTTC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 2 cut(s) 149, 293
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 5 cut(s) 94, 124, 264, 271, 468
BstMBI GATC 5 cut(s) 91, 121, 261, 268, 465
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 291
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 2 cut(s) 441, 528
Cac8I GCNNGC 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 76, 115
Csp6I GTAC 3 cut(s) 166, 397, 559
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 2 cut(s) 112, 173
CviQI GTAC 3 cut(s) 166, 397, 559
DdeI CTNAG 2 cut(s) 149, 293
DpnI GATC 5 cut(s) 93, 123, 263, 270, 467
DpnII GATC 5 cut(s) 91, 121, 261, 268, 465
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 115, 176
FaiI YATR 8 cut(s) 38, 113, 174, 260, 371, 413, 504, 522
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FatI CATG 2 cut(s) 111, 172
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 187, 305
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 2 cut(s) 187, 305
GluI GCNGC 2 cut(s) 187, 305
GsaI CCCAGC 2 cut(s) 311, 425
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 287, 461, 540
Hin1II CATG 2 cut(s) 115, 176
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 249
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 287, 461, 540
HphI GGTGA 5 cut(s) 213, 226, 268, 460, 475
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 209, 248, 470
Hpy188III TCNNGA 3 cut(s) 95, 103, 253
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 24, 59
HpyCH4III ACNGT 2 cut(s) 33, 418
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 1 cut(s) 494
HpyF3I CTNAG 2 cut(s) 149, 293
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 2 cut(s) 115, 176
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 5 cut(s) 91, 121, 261, 268, 465
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 3 cut(s) 289, 405, 579
Lsp1109I GCAGC 1 cut(s) 291
LweI GCATC 3 cut(s) 341, 447, 535
MaeII ACGT 1 cut(s) 570
MalI GATC 5 cut(s) 93, 123, 263, 270, 467
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 91, 121, 261, 268, 465
MboII GAAGA 4 cut(s) 48, 98, 152, 235
MhlI GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 39, 69
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 7 cut(s) 284, 306, 343, 420, 444, 464, 491
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 13
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 287, 461, 540
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 91, 121, 261, 268, 465
NlaIII CATG 2 cut(s) 115, 176
NlaIV GGNNCC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 2 cut(s) 188, 306
Ppu21I YACGTR 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 2 cut(s) 307, 421
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 2 cut(s) 76, 115
RsaI GTAC 3 cut(s) 167, 398, 560
RsaNI GTAC 3 cut(s) 166, 397, 559
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 187, 305
Sau3AI GATC 5 cut(s) 91, 121, 261, 268, 465
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
SfaNI GCATC 3 cut(s) 341, 447, 535
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 2 cut(s) 39, 69
SsiI CCGC 2 cut(s) 118, 186
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 33, 418
TaiI ACGT 1 cut(s) 573
TaqI TCGA 2 cut(s) 264, 299
TasI AATT 2 cut(s) 39, 69
TatI WGTACW 3 cut(s) 165, 396, 558
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 441, 535
TseI GCWGC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 25, 368, 386
TspRI CASTG 2 cut(s) 441, 535
VpaK11BI GGWCC 2 cut(s) 76, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.