Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AcsI | RAATTY | 3 cut(s) | 97, 183, 503 |
| AcuI | CTGAAG | 1 cut(s) | 207 |
| AfaI | GTAC | 1 cut(s) | 356 |
| AgsI | TTSAA | 4 cut(s) | 132, 212, 494, 503 |
| AhlI | ACTAGT | 1 cut(s) | 269 |
| AluBI | AGCT | 3 cut(s) | 400, 419, 464 |
| AluI | AGCT | 3 cut(s) | 400, 419, 464 |
| Alw26I | GTCTC | 4 cut(s) | 236, 289, 332, 442 |
| AoxI | GGCC | 2 cut(s) | 261, 467 |
| ApoI | RAATTY | 3 cut(s) | 97, 183, 503 |
| AspA2I | CCTAGG | 1 cut(s) | 358 |
| AspS9I | GGNCC | 1 cut(s) | 467 |
| AsuHPI | GGTGA | 2 cut(s) | 283, 322 |
| AvrII | CCTAGG | 1 cut(s) | 358 |
| AxyI | CCTNAGG | 1 cut(s) | 471 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BccI | CCATC | 2 cut(s) | 43, 272 |
| BcoDI | GTCTC | 4 cut(s) | 236, 289, 332, 442 |
| BcuI | ACTAGT | 1 cut(s) | 269 |
| BfaI | CTAG | 2 cut(s) | 270, 359 |
| BlnI | CCTAGG | 1 cut(s) | 358 |
| BmgT120I | GGNCC | 1 cut(s) | 467 |
| BmsI | GCATC | 1 cut(s) | 499 |
| BpuEI | CTTGAG | 1 cut(s) | 422 |
| BsaI | GGTCTC | 1 cut(s) | 332 |
| BsaJI | CCNNGG | 1 cut(s) | 358 |
| Bse21I | CCTNAGG | 1 cut(s) | 471 |
| Bse3DI | GCAATG | 1 cut(s) | 67 |
| BseDI | CCNNGG | 1 cut(s) | 358 |
| BseGI | GGATG | 1 cut(s) | 17 |
| BseMI | GCAATG | 1 cut(s) | 67 |
| BseMII | CTCAG | 3 cut(s) | 94, 219, 443 |
| BseRI | GAGGAG | 1 cut(s) | 460 |
| BseYI | CCCAGC | 1 cut(s) | 464 |
| BsgI | GTGCAG | 1 cut(s) | 231 |
| BshFI | GGCC | 2 cut(s) | 263, 469 |
| BsmAI | GTCTC | 4 cut(s) | 236, 289, 332, 442 |
| BsnI | GGCC | 2 cut(s) | 263, 469 |
| Bso31I | GGTCTC | 1 cut(s) | 332 |
| BspANI | GGCC | 2 cut(s) | 263, 469 |
| BspCNI | CTCAG | 3 cut(s) | 93, 218, 444 |
| BspTNI | GGTCTC | 1 cut(s) | 332 |
| BsrDI | GCAATG | 1 cut(s) | 67 |
| BssECI | CCNNGG | 1 cut(s) | 358 |
| BssT1I | CCWWGG | 1 cut(s) | 358 |
| Bst6I | CTCTTC | 4 cut(s) | 377, 405, 437, 489 |
| BstDEI | CTNAG | 4 cut(s) | 80, 205, 452, 471 |
| BstF5I | GGATG | 1 cut(s) | 17 |
| BstMAI | GTCTC | 4 cut(s) | 236, 289, 332, 442 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 227 |
| Bsu36I | CCTNAGG | 1 cut(s) | 471 |
| BsuRI | GGCC | 2 cut(s) | 263, 469 |
| BtsCI | GGATG | 1 cut(s) | 17 |
| Cfr13I | GGNCC | 1 cut(s) | 467 |
| Csp6I | GTAC | 1 cut(s) | 355 |
| CviAII | CATG | 1 cut(s) | 164 |
| CviJI | RGCY | 7 cut(s) | 11, 221, 263, 400, 419, 464, 469 |
| CviKI_1 | RGCY | 7 cut(s) | 11, 221, 263, 400, 419, 464, 469 |
| CviQI | GTAC | 1 cut(s) | 355 |
| DdeI | CTNAG | 4 cut(s) | 80, 205, 452, 471 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| Eam1104I | CTCTTC | 4 cut(s) | 377, 405, 437, 489 |
| EarI | CTCTTC | 4 cut(s) | 377, 405, 437, 489 |
| Eco130I | CCWWGG | 1 cut(s) | 358 |
| Eco31I | GGTCTC | 1 cut(s) | 332 |
| Eco57I | CTGAAG | 1 cut(s) | 207 |
| Eco81I | CCTNAGG | 1 cut(s) | 471 |
| EcoT14I | CCWWGG | 1 cut(s) | 358 |
| ErhI | CCWWGG | 1 cut(s) | 358 |
| FaeI | CATG | 1 cut(s) | 167 |
| FaiI | YATR | 3 cut(s) | 23, 165, 198 |
| FatI | CATG | 1 cut(s) | 163 |
| FokI | GGATG | 1 cut(s) | 4 |
| FspBI | CTAG | 2 cut(s) | 270, 359 |
| GsaI | CCCAGC | 1 cut(s) | 468 |
| HaeIII | GGCC | 2 cut(s) | 263, 469 |
| Hin1II | CATG | 1 cut(s) | 167 |
| HindIII | AAGCTT | 1 cut(s) | 398 |
| HinfI | GANTC | 1 cut(s) | 212 |
| HphI | GGTGA | 2 cut(s) | 283, 322 |
| Hpy166II | GTNNAC | 1 cut(s) | 355 |
| Hpy188I | TCNGA | 2 cut(s) | 83, 226 |
| Hpy8I | GTNNAC | 1 cut(s) | 355 |
| HpyAV | CCTTC | 1 cut(s) | 424 |
| HpyCH4IV | ACGT | 1 cut(s) | 194 |
| HpyCH4V | TGCA | 5 cut(s) | 200, 248, 317, 379, 512 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 227 |
| HpyF3I | CTNAG | 4 cut(s) | 80, 205, 452, 471 |
| HpySE526I | ACGT | 1 cut(s) | 194 |
| Hsp92II | CATG | 1 cut(s) | 167 |
| LpnPI | CCDG | 4 cut(s) | 101, 191, 236, 450 |
| LweI | GCATC | 1 cut(s) | 499 |
| MaeI | CTAG | 2 cut(s) | 270, 359 |
| MaeII | ACGT | 1 cut(s) | 194 |
| MaeIII | GTNAC | 2 cut(s) | 289, 364 |
| MboII | GAAGA | 6 cut(s) | 296, 394, 422, 439, 454, 506 |
| MfeI | CAATTG | 1 cut(s) | 237 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 7 cut(s) | 51, 74, 97, 124, 183, 237, 503 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MnlI | CCTC | 6 cut(s) | 124, 214, 378, 384, 406, 438 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 2 cut(s) | 146, 520 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MunI | CAATTG | 1 cut(s) | 237 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 227 |
| NlaIII | CATG | 1 cut(s) | 167 |
| NmuCI | GTSAC | 2 cut(s) | 289, 364 |
| PfeI | GAWTC | 1 cut(s) | 212 |
| PspFI | CCCAGC | 1 cut(s) | 464 |
| PspPI | GGNCC | 1 cut(s) | 467 |
| RsaI | GTAC | 1 cut(s) | 356 |
| RsaNI | GTAC | 1 cut(s) | 355 |
| SaqAI | TTAA | 2 cut(s) | 146, 520 |
| Sau96I | GGNCC | 1 cut(s) | 467 |
| SetI | ASST | 9 cut(s) | 146, 197, 206, 360, 364, 402, 421, 466, 492 |
| SfaNI | GCATC | 1 cut(s) | 499 |
| SmlI | CTYRAG | 1 cut(s) | 401 |
| SmoI | CTYRAG | 1 cut(s) | 401 |
| SpeI | ACTAGT | 1 cut(s) | 269 |
| Sse9I | AATT | 7 cut(s) | 51, 74, 97, 124, 183, 237, 503 |
| SspMI | CTAG | 2 cut(s) | 270, 359 |
| StyI | CCWWGG | 1 cut(s) | 358 |
| TaiI | ACGT | 1 cut(s) | 197 |
| TasI | AATT | 7 cut(s) | 51, 74, 97, 124, 183, 237, 503 |
| TfiI | GAWTC | 1 cut(s) | 212 |
| Tru1I | TTAA | 2 cut(s) | 146, 520 |
| Tru9I | TTAA | 2 cut(s) | 146, 520 |
| TseFI | GTSAC | 2 cut(s) | 289, 364 |
| Tsp45I | GTSAC | 2 cut(s) | 289, 364 |
| XapI | RAATTY | 3 cut(s) | 97, 183, 503 |
| XmaJI | CCTAGG | 1 cut(s) | 358 |
| XspI | CTAG | 2 cut(s) | 270, 359 |