MD11G1012500.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000726
Physical Location & Seq
Forward (+)
917096 .. 917671
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1012500.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGCTAGGCTGTCAGCTTTCCAATTCACAATTTTCTCCCTCCTCTCTTCCTTTGCCTTGATCTTAGTTTCTGGAGAAAATTTAGGGTTTCACAGCACCGTGGACCGTAAGCTTCTCGGGCTGCAGAAAGAAAAGCTCACCCACGTTCGCTTATACTGGCATGACATTGTTGGTGGGTCCCATCCCTCTGCCTTAGAGGTTGTCCCAGCACCCTCCAATAAGTCCCAGACGAATTTTGGGTTAGTGAGAATGTTTGATAATGCACTAACTGAAGGTCCTGAGTTGAGCTCAAAATTGGTGGCTAGGGCTCAAGGGTTTTACGGATTTGCAGGGCAGGTGGAGCTCAGCCTGCTGATGGCACAGAACTTTGCTTTTGTTGCAGGTGAGTTTAATGGAAGCACCATAACTTTGCTAGGAAGAAATTCAATTTTAAACAAGGTGAGGGAGCTGCCTGTGATTGGTGGAAGTGGGGCTTTCAGGTTTGCTAGGGGTTATGCTGAATTGAGAACTCACACTTTTAATCTCACCTCAAGTGATGCTATTGTGGAGTATAATATTTACGTGTTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.01

Weight (kDa)

8.96

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 47 - 188 2.3e-53 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 325, 371
Acc36I ACCTGC 2 cut(s) 325, 371
AcsI RAATTY 3 cut(s) 79, 232, 421
AcuI CTGAAG 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 355, 458
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 1 cut(s) 426
AluBI AGCT 6 cut(s) 17, 112, 136, 288, 343, 448
AluI AGCT 6 cut(s) 17, 112, 136, 288, 343, 448
Alw21I GWGCWC 2 cut(s) 290, 345
Ama87I CYCGRG 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 121, 448
ApoI RAATTY 3 cut(s) 79, 232, 421
Asp700I GAANNNNTTC 1 cut(s) 421
AspS9I GGNCC 3 cut(s) 103, 177, 275
AsuHPI GGTGA 4 cut(s) 130, 395, 451, 517
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 3 cut(s) 103, 177, 275
BanII GRGCYC 3 cut(s) 290, 310, 345
Bbv12I GWGCWC 2 cut(s) 290, 345
BbvI GCAGC 2 cut(s) 108, 435
BccI CCATC 2 cut(s) 189, 349
BfaI CTAG 4 cut(s) 6, 303, 413, 486
BfmI CTRYAG 1 cut(s) 122
BfuAI ACCTGC 2 cut(s) 325, 371
BisI GCNGC 2 cut(s) 122, 449
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 2 cut(s) 123, 450
Bme18I GGWCC 3 cut(s) 103, 177, 275
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 103, 177, 275
BmiI GGNNCC 2 cut(s) 178, 179
BmsI GCATC 1 cut(s) 526
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 344
BpuEI CTTGAG 2 cut(s) 294, 514
BsaAI YACGTR 1 cut(s) 562
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 458
Bse1I ACTGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 355, 458
BseMII CTCAG 2 cut(s) 270, 358
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 108, 435
BseYI CCCAGC 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 290, 345
BsiHKCI CYCGRG 1 cut(s) 116
BslFI GGGAC 3 cut(s) 163, 188, 208
BslI CCNNNNNNNGG 2 cut(s) 355, 458
BsmFI GGGAC 3 cut(s) 163, 188, 208
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 290, 310, 345
Bsp143I GATC 1 cut(s) 60
Bsp1720I GCTNAGC 1 cut(s) 344
BspCNI CTCAG 2 cut(s) 271, 357
BspLI GGNNCC 2 cut(s) 178, 179
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 2 cut(s) 325, 371
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 100, 107
Bst6I CTCTTC 1 cut(s) 52
BstBAI YACGTR 1 cut(s) 562
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 64, 193, 279, 344
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 181
BstKTI GATC 1 cut(s) 63
BstMBI GATC 1 cut(s) 60
BstMWI GCNNNNNNNGC 4 cut(s) 118, 340, 349, 377
BstSFI CTRYAG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 108, 435
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 181
BveI ACCTGC 2 cut(s) 325, 371
Cac8I GCNNGC 1 cut(s) 350
Cfr13I GGNCC 3 cut(s) 103, 177, 275
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 1 cut(s) 161
DdeI CTNAG 4 cut(s) 64, 193, 279, 344
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
DraI TTTAAA 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Ecl136II GAGCTC 2 cut(s) 288, 343
Eco24I GRGCYC 3 cut(s) 290, 310, 345
Eco47I GGWCC 3 cut(s) 103, 177, 275
Eco53kI GAGCTC 2 cut(s) 288, 343
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 2 cut(s) 288, 343
EcoO109I RGGNCCY 2 cut(s) 177, 275
EcoT38I GRGCYC 3 cut(s) 290, 310, 345
FaeI CATG 1 cut(s) 164
FaiI YATR 5 cut(s) 154, 162, 404, 495, 552
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FaqI GGGAC 3 cut(s) 163, 188, 208
FatI CATG 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 122, 449
FokI GGATG 1 cut(s) 168
FriOI GRGCYC 3 cut(s) 290, 310, 345
Fsp4HI GCNGC 2 cut(s) 122, 449
FspBI CTAG 4 cut(s) 6, 303, 413, 486
GluI GCNGC 2 cut(s) 122, 449
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 93
Hin1II CATG 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 110
HphI GGTGA 4 cut(s) 130, 395, 451, 517
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 2 cut(s) 72, 278
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 2 cut(s) 100, 107
HpyCH4IV ACGT 2 cut(s) 144, 561
HpyCH4V TGCA 5 cut(s) 124, 263, 329, 380, 568
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 340, 349, 377
HpyF3I CTNAG 4 cut(s) 64, 193, 279, 344
HpySE526I ACGT 2 cut(s) 144, 561
Hsp92II CATG 1 cut(s) 164
KflI GGGWCCC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 60
LmnI GCTCC 2 cut(s) 340, 445
Lsp1109I GCAGC 2 cut(s) 108, 435
LweI GCATC 1 cut(s) 526
MaeI CTAG 4 cut(s) 6, 303, 413, 486
MaeII ACGT 2 cut(s) 144, 561
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 2 cut(s) 39, 429
MhlI GDGCHC 3 cut(s) 290, 310, 345
MluCI AATT 8 cut(s) 23, 30, 79, 232, 293, 421, 426, 500
MnlI CCTC 7 cut(s) 50, 53, 190, 196, 223, 435, 538
MroXI GAANNNNTTC 1 cut(s) 421
MseI TTAA 3 cut(s) 390, 431, 519
MwoI GCNNNNNNNGC 4 cut(s) 118, 340, 349, 377
NdeII GATC 1 cut(s) 60
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 2 cut(s) 178, 179
PaqCI CACCTGC 2 cut(s) 325, 371
PdmI GAANNNNTTC 1 cut(s) 421
PkrI GCNGC 2 cut(s) 123, 450
Ppu21I YACGTR 1 cut(s) 562
PpuMI RGGWCCY 2 cut(s) 177, 275
Psp124BI GAGCTC 2 cut(s) 290, 345
Psp5II RGGWCCY 2 cut(s) 177, 275
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 178, 179
PspPI GGNCC 3 cut(s) 103, 177, 275
PspPPI RGGWCCY 2 cut(s) 177, 275
PstI CTGCAG 1 cut(s) 126
SacI GAGCTC 2 cut(s) 290, 345
SaqAI TTAA 3 cut(s) 390, 431, 519
SatI GCNGC 2 cut(s) 122, 449
Sau3AI GATC 1 cut(s) 60
Sau96I GGNCC 3 cut(s) 103, 177, 275
SduI GDGCHC 3 cut(s) 290, 310, 345
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 122
SinI GGWCC 3 cut(s) 103, 177, 275
SmlI CTYRAG 2 cut(s) 309, 529
SmoI CTYRAG 2 cut(s) 309, 529
Sse9I AATT 8 cut(s) 23, 30, 79, 232, 293, 421, 426, 500
SspI AATATT 1 cut(s) 556
SspMI CTAG 4 cut(s) 6, 303, 413, 486
SstI GAGCTC 2 cut(s) 290, 345
TaaI ACNGT 2 cut(s) 100, 107
TaiI ACGT 2 cut(s) 147, 564
TasI AATT 8 cut(s) 23, 30, 79, 232, 293, 421, 426, 500
Tru1I TTAA 3 cut(s) 390, 431, 519
Tru9I TTAA 3 cut(s) 390, 431, 519
TseI GCWGC 2 cut(s) 121, 448
TspGWI ACGGA 1 cut(s) 336
VpaK11BI GGWCC 3 cut(s) 103, 177, 275
XapI RAATTY 3 cut(s) 79, 232, 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 421
XspI CTAG 4 cut(s) 6, 303, 413, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.