Rh5DG562000

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000726
Physical Location & Seq
Reverse (-)
86662967 .. 86663557
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG562000.1

Sequence Viewer

Length: 591 bp
ATGGCTAGAATCCTTCCTATCTCAGCTTTCCAATTCATCATAATCTCCTCCCTCCTGTCTTCCTTTTCTATGATCATAGTTTCTGGGGAAGATCATGAGTTTGTGAAACCCATGGACCCCGAGCTTTTTGGTCTAAAGAAAGAAAAACTTACCCATTTTCGCTTGTATTGGCACGACATTGTTGATGGTCCTAATCCCACTGCCGTGACAGTAGTGCAACCACTCCCCAACTCCTCCCAAACAAAGTTCGGGATGATAAGAATGATCGACAACCAGCTAACCTTAGGCCCCAAATCGAGCTCGAAGCTGTTGGGAAGGGCTCAAGGGTTTTATGCTTCTGCATCACAAGAAGACCCTAGTCTGTTGATGGCTCAGAACTTTGCATTTGTTCAGGGGAAGTATAACGGTAGCGCCATAACCCTGATGGGAAGGAACTCGCTTTCCAACAAGGTAAGGGAGCTGTCGGTGATCGGAGGAAGTGGACTTTTCAGGTTTGCTAGGGGTTATGCTCTACTAACAACTCGGTCGTTTAACGCCTCTAAAAGTGATGATGCTGTGGTGGAGTATAACATCTATGTCTTGCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

196

Amino Acids

21.74

Weight (kDa)

9.33

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 193 2.5e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AleI CACNNNNGTG 1 cut(s) 203
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 6 cut(s) 26, 124, 277, 300, 307, 460
AluI AGCT 6 cut(s) 26, 124, 277, 300, 307, 460
Alw21I GWGCWC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 286
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 3 cut(s) 115, 188, 287
AsuHPI GGTGA 1 cut(s) 478
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 2 cut(s) 115, 188
AxyI CCTNAGG 1 cut(s) 283
BanII GRGCYC 2 cut(s) 302, 322
BbsI GAAGAC 2 cut(s) 51, 357
Bbv12I GWGCWC 1 cut(s) 302
BccI CCATC 3 cut(s) 179, 361, 418
BceAI ACGGC 1 cut(s) 188
BclI TGATCA 1 cut(s) 72
BfaI CTAG 3 cut(s) 6, 357, 498
BfoI RGCGCY 1 cut(s) 414
Bme18I GGWCC 2 cut(s) 115, 188
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 115, 188, 287
BmiI GGNNCC 2 cut(s) 117, 289
BmsI GCATC 2 cut(s) 350, 541
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 51, 357
BpuEI CTTGAG 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 111
Bse21I CCTNAGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 36, 386
BseRI GAGGAG 2 cut(s) 37, 223
Bsh1285I CGRYCG 1 cut(s) 527
BshFI GGCC 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 119
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 302, 322
Bsp143I GATC 4 cut(s) 72, 91, 264, 468
Bsp19I CCATGG 1 cut(s) 111
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 2 cut(s) 35, 385
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 117, 289
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 72, 91, 264, 468
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 211, 407
BstDEI CTNAG 3 cut(s) 22, 283, 372
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 4 cut(s) 75, 94, 267, 471
BstMBI GATC 4 cut(s) 72, 91, 264, 468
BstMCI CGRYCG 1 cut(s) 527
BstPAI GACNNNNGTC 1 cut(s) 357
BstV2I GAAGAC 2 cut(s) 51, 357
Bsu36I CCTNAGG 1 cut(s) 283
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 258
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 115, 188, 287
CviAII CATG 2 cut(s) 95, 112
DdeI CTNAG 3 cut(s) 22, 283, 372
DpnI GATC 4 cut(s) 74, 93, 266, 470
DpnII GATC 4 cut(s) 72, 91, 264, 468
Ecl136II GAGCTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 302, 322
Eco47I GGWCC 2 cut(s) 115, 188
Eco53kI GAGCTC 1 cut(s) 300
Eco81I CCTNAGG 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 2 cut(s) 302, 322
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 98, 115
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FatI CATG 2 cut(s) 94, 111
FbaI TGATCA 1 cut(s) 72
FokI GGATG 1 cut(s) 265
FriOI GRGCYC 2 cut(s) 302, 322
FspBI CTAG 3 cut(s) 6, 357, 498
GlaI GCGC 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 1 cut(s) 288
HhaI GCGC 1 cut(s) 413
Hin1II CATG 2 cut(s) 98, 115
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HinfI GANTC 1 cut(s) 9
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 482
Hpy188I TCNGA 2 cut(s) 375, 473
Hpy188III TCNNGA 2 cut(s) 95, 250
Hpy8I GTNNAC 1 cut(s) 482
HpyAV CCTTC 3 cut(s) 23, 309, 423
HpyCH4III ACNGT 2 cut(s) 211, 407
HpyCH4V TGCA 4 cut(s) 217, 341, 383, 583
HpyF3I CTNAG 3 cut(s) 22, 283, 372
Hsp92II CATG 2 cut(s) 98, 115
HspAI GCGC 1 cut(s) 411
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 4 cut(s) 72, 91, 264, 468
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 6 cut(s) 68, 69, 287, 377, 434, 475
LweI GCATC 2 cut(s) 350, 541
MaeI CTAG 3 cut(s) 6, 357, 498
MaeIII GTNAC 1 cut(s) 205
MalI GATC 4 cut(s) 74, 93, 266, 470
MboI GATC 4 cut(s) 72, 91, 264, 468
MboII GAAGA 3 cut(s) 51, 101, 362
MhlI GDGCHC 2 cut(s) 302, 322
MluCI AATT 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 5 cut(s) 58, 62, 244, 467, 547
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 72, 91, 264, 468
NlaIII CATG 2 cut(s) 98, 115
NlaIV GGNNCC 2 cut(s) 117, 289
NmuCI GTSAC 1 cut(s) 205
OliI CACNNNNGTG 1 cut(s) 203
PagI TCATGA 1 cut(s) 94
PfeI GAWTC 1 cut(s) 9
PshAI GACNNNNGTC 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 117, 289
PspPI GGNCC 3 cut(s) 115, 188, 287
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 4 cut(s) 72, 91, 264, 468
Sau96I GGNCC 3 cut(s) 115, 188, 287
SduI GDGCHC 2 cut(s) 302, 322
SetI ASST 9 cut(s) 28, 126, 279, 284, 302, 309, 453, 462, 494
SfaNI GCATC 2 cut(s) 350, 541
SinI GGWCC 2 cut(s) 115, 188
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 1 cut(s) 32
SspMI CTAG 3 cut(s) 6, 357, 498
SstI GAGCTC 1 cut(s) 302
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 211, 407
TaqI TCGA 3 cut(s) 267, 296, 302
TaqII GACCGA 1 cut(s) 513
TasI AATT 1 cut(s) 32
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 115, 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 3 cut(s) 6, 357, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.