FvH4_4g08040

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001253
Physical Location & Seq
Forward (+)
7637411 .. 7645474
8064 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08040.t3

Sequence Viewer

Length: 2655 bp
ATGGTGGATGTATCTAGTGCTGGGAAGGCAGGTGGGGAACTAGATTTGGTCCGGCTATCTCCTCCACCAATTCCACCCAATGTGAAGCCAGAAGTCCTGATTTCTCCCAAGTATTTTATTATGACTAGGCGTGGTACTGGAACAACTGGGAGACGCATACAATTGCTCGCAAACCACTTCAAAGTCACAGTGAAAGCTCCAGATGCTGTGTTCTTCCAGTATTCTGTTCATATTACTGCTGAAGACAAGAGAGCCGTTGAAGGCAAGGGAATTGGAAGAAAACTGGTTGACAGGCTTTTTCAAACATATTCTTCCGAACTAGCTGGTAAAAAGTTTGCGTATGATGGGGAGAAAGCTCTGTATACGGTTGGCCCCCTGCCACAGAACAAGCTTGAATTCACGGTTGTGCTGGAGGAGACATTTGCAAAGAGTGAAACTGTGAGTCCCGGTGAAGGGACTGAAAAAAGGTCAAGACGAACTCTTCAGTCAAAAACTTTCACAGTAGAGTTAAGTTATGCTGCCAAAATACCTTTGAAGTCAATTGCTCTTGCCCTCAAAGGAGCCAATGTCGACAATACTCAGGATGCATTGAGAGTGCTTGATATTATACTACGGCAGCAAGCAGCTAACATGGGATGCCTTCTAGTACGACAGTCATTCTTTCATGATGACTCCAGGAACTTTACTGATGTTGGAGCAGGTGTGATTGGTGTTCGGGGATTTCATTCCAGCTTCCGTCCAACTCAGGGTGGTTTGTCCTTGAATATGGATGTATCTACAACAATGATCTTGACCCCTGGACCAGTAATAGATTTTCTGATTGCCAACCAGGGTGTGCGAGAACCCCGCTACATTGACTGGGTAAAGGCCAAGAGAATGCTGAAAAACATGAGGATTAAGACGAGACACCGTAACATGGAATTTAAAATAATAGGCATCAGTGAGAAGCCCTGCAATCTGCAATGCTTTCCTATGAAATTAAAAACTGGTGATGGTGCAAGTGAAGGGGAGACTGCAGACATTACTGTGTTTGAGTACTTCAGCAAACACTGTGGCATTGAGCTCACATACTCCCAATACCTGCCATGCCTTGATGTTGGCAAACCAAAAAGGCCCAACTATCTGCCTGTGGAGCTCTGTGCGCTTGTTTCATTACAACGGTATACAAAAGCATTATCTTCACAGCAGAGGGCATCCTTAGTTGAAAAGTCAAGGCAGAAGCCTCAGGATAGAATACGAACTGTGACTGATGCTGTATGCAAATATCAATATGATAGTGATCCTGTGCTTACTACATGCGGTATCTCAATAGAGAAGCAATTGACTCAGTTTGAAGGACGCGTTCTTGATACTCCAAAGTTAAAGGTAGGTAACAGTGAGGATTGCACCCCTTTCAAGGGACGATGGAACTTCAACAACAAGACACTTTTGAACCCCACTCGCATTGACCGCTGGATTGTTGTCAACTTCTCTGCACGGTGTGATACCAGCCAAATCTCGAGGGACCTTATCAACTGTGGAAGGAAAAAAGGCATTCTTATTGAGCGTCCCTTTACGCTGCTTGAGGAAGAACCACAGTGTAGAAGACAAAGCCCTGTTGTAAGAGTTGAAAAGATGTTTGAACAGATTCAAACTAAGCTTCCAAGTCCACCTGACTTTATTCTTTGTGTTCTACCAGAAAAGAAAAACTCAGATATCTATGGGCCTTGGAAGAAAAGGACTCTCAGTGACTTCGGAATTGTTACACAGTGCATTTCCCCTTCAAAGCTTGGAGGAATAAATTCTTTGTTGGCACTTGAGCACTCCTCACGTGTCCCTCTGATAAAAGATACTCCTACAATGATTCTGGGAATGGATGTGTCTCATGGTTCTCCTGGCCGATCAGATGTTCCTTCTGTTGCTGCGGTTGTTGGCTCTCGGAGTTGGCCACTTATTTCAAGGTACAGAGCAGCAGTTAGAACACAATCCCCTAAGGTGGAAATGATTGATGCTCTGTATAAGCCACTGGCAAATGGAAAAGATGATGGTATTATCAGGGAACTTCTTGTCGACTTCTTTAAGACAAGCAATGGACGCAAACCAACGCAGATTATTGTATTCAGAGATGGCGTAAGTGAATCACAGTTTAATCAAGTCCTGAACATTGAGCTGGATCAGATCGTAAAGGCTTACCAGCATCTGGGGGAGGCTAATATTCCAAAGTTTACTGTGATTGTGGCTCAGAAGAATCATCACACAAAGCTATTCCAAACTAATTCACCTGATAATGTTCCACCAGGGACTGTTGTTGATACCAAAATTGTGCATCCAAGGAATTATGATTTCTACATGTGTGCTCAGGCAGGCATGATTGGAACTTCCAGGCCAGCTCACTATCATGTCTTGCTTGATGAGATTGGTTTTTCCCCTGATGACTTGCAAAACCTGATTCACTCCCTTTCTTATGTGTACCAGAGGAGTACAACTGCAATCTCTATTGTGGCGCCCGTATGCTATGCTCACCTTGCTGCAGCACAAGTGGGGCAGTTCATGAAGTTTGAAGATCTCTCAGACACCTCATCGGGGAAGGGCAGTATCACTTCATCAGAGAGCATCCCAGTTCAAGAGCTCCCTAGGCTCCATGAGAAGGTCCAGGGCTCCATGTTCTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

885

Amino Acids

98.63

Weight (kDa)

9.26

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoL1 PF08699 80 - 127 2e-17 Argonaute linker 1 domain
PAZ PF02170 134 - 264 6.3e-30 PAZ domain
ArgoL2 PF16488 274 - 319 1.7e-09 Argonaute linker 2 domain
Piwi PF02171 417 - 725 5.7e-107 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 20, 689
Acc36I ACCTGC 3 cut(s) 20, 689, 1089
AccB1I GGYRCC 1 cut(s) 2482
AccB7I CCANNNNNTGG 1 cut(s) 2179
AccI GTMKAC 4 cut(s) 362, 570, 1163, 2049
AccII CGCG 1 cut(s) 1341
AciI CCGC 4 cut(s) 847, 1299, 1450, 1904
AclWI GGATC 2 cut(s) 1274, 2160
AcoI YGGCCR 2 cut(s) 1876, 1925
AcsI RAATTY 3 cut(s) 395, 920, 1780
AcuI CTGAAG 3 cut(s) 261, 467, 1024
AcvI CACGTG 1 cut(s) 1811
AcyI GRCGYC 1 cut(s) 2483
AdeI CACNNNGTG 1 cut(s) 1481
AfaI GTAC 6 cut(s) 136, 648, 1037, 1943, 2450, 2461
AflIII ACRYGT 3 cut(s) 1339, 1810, 2328
AjnI CCWGG 7 cut(s) 674, 796, 828, 1873, 2275, 2360, 2631
AleI CACNNNNGTG 1 cut(s) 404
Alw21I GWGCWC 5 cut(s) 1065, 1137, 1803, 2338, 2610
Alw26I GTCTC 5 cut(s) 145, 410, 898, 1004, 1866
AlwI GGATC 2 cut(s) 1274, 2160
AlwNI CAGNNNCTG 3 cut(s) 206, 2179, 2282
Ama87I CYCGRG 1 cut(s) 1498
AoxI GGCC 7 cut(s) 370, 867, 1112, 1703, 1876, 1925, 2363
ApeKI GCWGC 8 cut(s) 518, 616, 623, 1558, 1901, 1949, 2507, 2510
ApoI RAATTY 3 cut(s) 395, 920, 1780
Asp700I GAANNNNTTC 2 cut(s) 1626, 1780
AspA2I CCTAGG 1 cut(s) 2612
AspLEI GCGC 2 cut(s) 1144, 2485
AspS9I GGNCC 7 cut(s) 49, 371, 800, 1113, 1504, 1703, 2629
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 4 cut(s) 461, 1001, 2250, 2492
AvaI CYCGRG 1 cut(s) 1498
AvaII GGWCC 4 cut(s) 49, 800, 1504, 2629
AvrII CCTAGG 1 cut(s) 2612
AxyI CCTNAGG 2 cut(s) 1224, 1971
BalI TGGCCA 1 cut(s) 1927
BanI GGYRCC 1 cut(s) 2482
BanII GRGCYC 4 cut(s) 1065, 1137, 2610, 2639
BbrPI CACGTG 1 cut(s) 1811
BbsI GAAGAC 2 cut(s) 249, 1591
Bbv12I GWGCWC 5 cut(s) 1065, 1137, 1803, 2338, 2610
BbvI GCAGC 8 cut(s) 505, 628, 635, 1545, 1888, 1961, 2494, 2522
BccI CCATC 5 cut(s) 338, 986, 1398, 2018, 2099
BceAI ACGGC 2 cut(s) 239, 629
BciT130I CCWGG 7 cut(s) 676, 798, 830, 1875, 2277, 2362, 2633
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 5 cut(s) 145, 410, 898, 1004, 1866
BfaI CTAG 6 cut(s) 15, 41, 126, 320, 644, 2613
BfmI CTRYAG 2 cut(s) 1014, 2508
BfoI RGCGCY 1 cut(s) 2486
BfuAI ACCTGC 3 cut(s) 20, 689, 1089
BglII AGATCT 1 cut(s) 2542
BisI GCNGC 8 cut(s) 519, 617, 624, 1559, 1902, 1950, 2508, 2511
BlnI CCTAGG 1 cut(s) 2612
BlsI GCNGC 8 cut(s) 520, 618, 625, 1560, 1903, 1951, 2509, 2512
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 8 cut(s) 447, 676, 798, 830, 1875, 2277, 2362, 2633
Bme18I GGWCC 4 cut(s) 49, 800, 1504, 2629
BmeT110I CYCGRG 1 cut(s) 1498
BmgT120I GGNCC 7 cut(s) 49, 371, 800, 1113, 1504, 1703, 2629
BmiI GGNNCC 6 cut(s) 373, 562, 1505, 2484, 2618, 2638
BmrFI CCNGG 8 cut(s) 447, 676, 798, 830, 1875, 2277, 2362, 2633
BmrI ACTGGG 3 cut(s) 156, 868, 2591
BmuI ACTGGG 3 cut(s) 156, 868, 2591
BpiI GAAGAC 2 cut(s) 249, 1591
BplI GAGNNNNNCTC 2 cut(s) 1790, 1822
BpmI CTGGAG 3 cut(s) 183, 431, 658
Bpu10I CCTNAGC 1 cut(s) 2337
BpuEI CTTGAG 2 cut(s) 1583, 1817
BpuMI CCSGG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 1811
BsaBI GATNNNNATC 1 cut(s) 1278
BsaHI GRCGYC 1 cut(s) 2483
BsaJI CCNNGG 7 cut(s) 796, 829, 1706, 2276, 2309, 2612, 2632
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 687, 717
Bse1I ACTGG 9 cut(s) 142, 151, 217, 288, 803, 863, 991, 2010, 2597
Bse21I CCTNAGG 2 cut(s) 1224, 1971
Bse3DI GCAATG 2 cut(s) 968, 2074
Bse8I GATNNNNATC 1 cut(s) 1278
BseBI CCWGG 7 cut(s) 676, 798, 830, 1875, 2277, 2362, 2633
BseDI CCNNGG 7 cut(s) 796, 829, 1706, 2276, 2309, 2612, 2632
BseGI GGATG 8 cut(s) 13, 589, 641, 775, 1193, 1861, 2305, 2592
BseJI GATNNNNATC 1 cut(s) 1278
BseMI GCAATG 2 cut(s) 968, 2074
BseMII CTCAG 9 cut(s) 593, 758, 1238, 1340, 1704, 1738, 2234, 2351, 2562
BseNI ACTGG 9 cut(s) 142, 151, 217, 288, 803, 863, 991, 2010, 2597
BseRI GAGGAG 4 cut(s) 51, 428, 1795, 2470
BseXI GCAGC 8 cut(s) 505, 628, 635, 1545, 1888, 1961, 2494, 2522
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 1458
Bsh1236I CGCG 1 cut(s) 1341
BshFI GGCC 7 cut(s) 372, 869, 1114, 1705, 1878, 1927, 2365
BshNI GGYRCC 1 cut(s) 2482
BsiHKAI GWGCWC 5 cut(s) 1065, 1137, 1803, 2338, 2610
BsiHKCI CYCGRG 1 cut(s) 1498
BsiSI CCGG 2 cut(s) 52, 447
BslFI GGGAC 7 cut(s) 429, 469, 1413, 1517, 1533, 1799, 2293
BsmAI GTCTC 5 cut(s) 145, 410, 898, 1004, 1866
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 7 cut(s) 429, 469, 1413, 1517, 1533, 1799, 2293
BsmI GAATGC 2 cut(s) 882, 1533
BsnI GGCC 7 cut(s) 372, 869, 1114, 1705, 1878, 1927, 2365
BsoBI CYCGRG 1 cut(s) 1498
Bsp1286I GDGCHC 6 cut(s) 1065, 1137, 1803, 2338, 2610, 2639
Bsp143I GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
BspACI CCGC 4 cut(s) 847, 1299, 1450, 1904
BspANI GGCC 7 cut(s) 372, 869, 1114, 1705, 1878, 1927, 2365
BspCNI CTCAG 9 cut(s) 592, 757, 1237, 1339, 1703, 1737, 2233, 2350, 2561
BspFNI CGCG 1 cut(s) 1341
BspHI TCATGA 2 cut(s) 664, 2529
BspLI GGNNCC 6 cut(s) 373, 562, 1505, 2484, 2618, 2638
BspMAI CTGCAG 2 cut(s) 1018, 2512
BspMI ACCTGC 3 cut(s) 20, 689, 1089
BspPI GGATC 2 cut(s) 1274, 2160
BspT107I GGYRCC 1 cut(s) 2482
BsrDI GCAATG 2 cut(s) 968, 2074
BsrI ACTGG 9 cut(s) 142, 151, 217, 288, 803, 863, 991, 2010, 2597
BssECI CCNNGG 7 cut(s) 796, 829, 1706, 2276, 2309, 2612, 2632
BssMI GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
BssNAI GTATAC 2 cut(s) 363, 1164
BssNI GRCGYC 1 cut(s) 2483
BssT1I CCWWGG 3 cut(s) 1706, 2309, 2612
Bst1107I GTATAC 2 cut(s) 363, 1164
Bst2UI CCWGG 7 cut(s) 676, 798, 830, 1875, 2277, 2362, 2633
Bst6I CTCTTC 1 cut(s) 486
BstACI GRCGYC 1 cut(s) 2483
BstBAI YACGTR 1 cut(s) 1811
BstC8I GCNNGC 4 cut(s) 168, 621, 2344, 2367
BstF5I GGATG 8 cut(s) 13, 589, 641, 775, 1193, 1861, 2305, 2592
BstFNI CGCG 1 cut(s) 1341
BstH2I RGCGCY 1 cut(s) 2486
BstHHI GCGC 2 cut(s) 1144, 2485
BstKTI GATC 6 cut(s) 789, 1282, 1883, 2155, 2160, 2545
BstMAI GTCTC 5 cut(s) 145, 410, 898, 1004, 1866
BstMBI GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
BstMWI GCNNNNNNNGC 8 cut(s) 26, 203, 1132, 1141, 1449, 2073, 2504, 2614
BstNI CCWGG 7 cut(s) 676, 798, 830, 1875, 2277, 2362, 2633
BstNSI RCATGY 2 cut(s) 1299, 2332
BstSCI CCNGG 8 cut(s) 445, 674, 796, 828, 1873, 2275, 2360, 2631
BstSFI CTRYAG 2 cut(s) 1014, 2508
BstUI CGCG 1 cut(s) 1341
BstV1I GCAGC 8 cut(s) 505, 628, 635, 1545, 1888, 1961, 2494, 2522
BstV2I GAAGAC 2 cut(s) 249, 1591
BstX2I RGATCY 1 cut(s) 2542
BstYI RGATCY 1 cut(s) 2542
BstZ17I GTATAC 2 cut(s) 363, 1164
Bsu36I CCTNAGG 2 cut(s) 1224, 1971
BsuRI GGCC 7 cut(s) 372, 869, 1114, 1705, 1878, 1927, 2365
BtsCI GGATG 8 cut(s) 13, 589, 641, 775, 1193, 1861, 2305, 2592
BtsIMutI CAGTG 8 cut(s) 195, 946, 1048, 1381, 1583, 1732, 1754, 2003
BveI ACCTGC 3 cut(s) 20, 689, 1089
Cac8I GCNNGC 4 cut(s) 168, 621, 2344, 2367
CaiI CAGNNNCTG 3 cut(s) 206, 2179, 2282
CciI TCATGA 2 cut(s) 664, 2529
CfoI GCGC 2 cut(s) 1144, 2485
Cfr13I GGNCC 7 cut(s) 49, 371, 800, 1113, 1504, 1703, 2629
CseI GACGC 4 cut(s) 162, 1347, 1535, 2082
Csp6I GTAC 6 cut(s) 135, 647, 1036, 1942, 2449, 2460
CspCI CAANNNNNGTGG 4 cut(s) 1033, 1068, 1426, 1461
CviQI GTAC 6 cut(s) 135, 647, 1036, 1942, 2449, 2460
DinI GGCGCC 1 cut(s) 2484
DpnI GATC 6 cut(s) 788, 1281, 1882, 2154, 2159, 2544
DpnII GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
DraI TTTAAA 1 cut(s) 925
DraIII CACNNNGTG 1 cut(s) 1481
EaeI YGGCCR 2 cut(s) 1876, 1925
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
Ecl136II GAGCTC 3 cut(s) 1063, 1135, 2608
Eco130I CCWWGG 3 cut(s) 1706, 2309, 2612
Eco24I GRGCYC 4 cut(s) 1065, 1137, 2610, 2639
Eco32I GATATC 1 cut(s) 1696
Eco47I GGWCC 4 cut(s) 49, 800, 1504, 2629
Eco53kI GAGCTC 3 cut(s) 1063, 1135, 2608
Eco57I CTGAAG 3 cut(s) 261, 467, 1024
Eco72I CACGTG 1 cut(s) 1811
Eco81I CCTNAGG 2 cut(s) 1224, 1971
Eco88I CYCGRG 1 cut(s) 1498
EcoICRI GAGCTC 3 cut(s) 1063, 1135, 2608
EcoO109I RGGNCCY 1 cut(s) 1504
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 7 cut(s) 674, 796, 828, 1873, 2275, 2360, 2631
EcoRV GATATC 1 cut(s) 1696
EcoT14I CCWWGG 3 cut(s) 1706, 2309, 2612
EcoT22I ATGCAT 1 cut(s) 589
EcoT38I GRGCYC 4 cut(s) 1065, 1137, 2610, 2639
EgeI GGCGCC 1 cut(s) 2484
EheI GGCGCC 1 cut(s) 2484
ErhI CCWWGG 3 cut(s) 1706, 2309, 2612
Esp3I CGTCTC 1 cut(s) 145
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 7 cut(s) 429, 469, 1413, 1517, 1533, 1799, 2293
FauI CCCGC 1 cut(s) 854
FblI GTMKAC 4 cut(s) 362, 570, 1163, 2049
Fnu4HI GCNGC 8 cut(s) 519, 617, 624, 1559, 1902, 1950, 2508, 2511
FokI GGATG 8 cut(s) 20, 596, 648, 782, 1180, 1868, 2292, 2579
FriOI GRGCYC 4 cut(s) 1065, 1137, 2610, 2639
Fsp4HI GCNGC 8 cut(s) 519, 617, 624, 1559, 1902, 1950, 2508, 2511
FspBI CTAG 6 cut(s) 15, 41, 126, 320, 644, 2613
GlaI GCGC 2 cut(s) 1143, 2484
GluI GCNGC 8 cut(s) 519, 617, 624, 1559, 1902, 1950, 2508, 2511
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 3 cut(s) 183, 431, 658
HaeII RGCGCY 1 cut(s) 2486
HaeIII GGCC 7 cut(s) 372, 869, 1114, 1705, 1878, 1927, 2365
HapII CCGG 2 cut(s) 52, 447
HgaI GACGC 4 cut(s) 162, 1347, 1535, 2082
HhaI GCGC 2 cut(s) 1144, 2485
Hin1I GRCGYC 1 cut(s) 2483
Hin6I GCGC 2 cut(s) 1142, 2483
HinP1I GCGC 2 cut(s) 1142, 2483
HincII GTYRAC 4 cut(s) 289, 571, 1465, 2050
HindII GTYRAC 4 cut(s) 289, 571, 1465, 2050
HindIII AAGCTT 3 cut(s) 389, 1637, 1766
HinfI GANTC 9 cut(s) 442, 671, 1324, 1627, 1720, 1843, 2117, 2227, 2428
HpaII CCGG 2 cut(s) 52, 447
HphI GGTGA 4 cut(s) 461, 1001, 2250, 2492
Hpy166II GTNNAC 9 cut(s) 289, 363, 571, 1164, 1465, 1649, 2050, 2205, 2449
Hpy8I GTNNAC 9 cut(s) 289, 363, 571, 1164, 1465, 1649, 2050, 2205, 2449
HpyCH4IV ACGT 1 cut(s) 1810
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 203, 1132, 1141, 1449, 2073, 2504, 2614
HpySE526I ACGT 1 cut(s) 1810
Hsp92I GRCGYC 1 cut(s) 2483
HspAI GCGC 2 cut(s) 1142, 2483
KasI GGCGCC 1 cut(s) 2482
Kzo9I GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
LmnI GCTCC 7 cut(s) 202, 560, 695, 1132, 2613, 2622, 2642
Lsp1109I GCAGC 8 cut(s) 505, 628, 635, 1545, 1888, 1961, 2494, 2522
MaeI CTAG 6 cut(s) 15, 41, 126, 320, 644, 2613
MaeII ACGT 1 cut(s) 1810
MaeIII GTNAC 6 cut(s) 184, 911, 1243, 1370, 1727, 1741
MalI GATC 6 cut(s) 788, 1281, 1882, 2154, 2159, 2544
MboI GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
MfeI CAATTG 3 cut(s) 161, 540, 1319
MflI RGATCY 1 cut(s) 2542
MhlI GDGCHC 6 cut(s) 1065, 1137, 1803, 2338, 2610, 2639
MlsI TGGCCA 1 cut(s) 1927
MluI ACGCGT 1 cut(s) 1339
MluNI TGGCCA 1 cut(s) 1927
Mly113I GGCGCC 1 cut(s) 2483
MlyI GAGTC 4 cut(s) 451, 665, 1318, 1714
MmeI TCCRAC 2 cut(s) 673, 764
Mox20I TGGCCA 1 cut(s) 1927
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 1626, 1780
MscI TGGCCA 1 cut(s) 1927
MseI TTAA 7 cut(s) 509, 897, 924, 980, 1361, 2058, 2127
MslI CAYNNNNRTG 3 cut(s) 404, 1025, 2376
Msp20I TGGCCA 1 cut(s) 1927
MspA1I CMGCKG 1 cut(s) 1452
MspI CCGG 2 cut(s) 52, 447
MspR9I CCNGG 8 cut(s) 447, 676, 798, 830, 1875, 2277, 2362, 2633
MunI CAATTG 3 cut(s) 161, 540, 1319
Mva1269I GAATGC 2 cut(s) 882, 1533
MvaI CCWGG 7 cut(s) 676, 798, 830, 1875, 2277, 2362, 2633
MvnI CGCG 1 cut(s) 1341
MwoI GCNNNNNNNGC 8 cut(s) 26, 203, 1132, 1141, 1449, 2073, 2504, 2614
NarI GGCGCC 1 cut(s) 2483
NciI CCSGG 1 cut(s) 447
NdeII GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
NlaIV GGNNCC 6 cut(s) 373, 562, 1505, 2484, 2618, 2638
NmuCI GTSAC 3 cut(s) 184, 1243, 1727
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 2 cut(s) 1299, 2332
OliI CACNNNNGTG 1 cut(s) 404
PaeR7I CTCGAG 1 cut(s) 1498
PagI TCATGA 2 cut(s) 664, 2529
PaqCI CACCTGC 2 cut(s) 20, 689
PciI ACATGT 1 cut(s) 2328
PctI GAATGC 2 cut(s) 882, 1533
PdmI GAANNNNTTC 2 cut(s) 1626, 1780
PfeI GAWTC 5 cut(s) 1627, 1843, 2117, 2227, 2428
PflMI CCANNNNNTGG 1 cut(s) 2179
PfoI TCCNGGA 1 cut(s) 674
PkrI GCNGC 8 cut(s) 520, 618, 625, 1560, 1903, 1951, 2509, 2512
PleI GAGTC 4 cut(s) 450, 665, 1318, 1714
PluTI GGCGCC 1 cut(s) 2486
PmaCI CACGTG 1 cut(s) 1811
PmlI CACGTG 1 cut(s) 1811
PpsI GAGTC 4 cut(s) 450, 665, 1318, 1714
Ppu21I YACGTR 1 cut(s) 1811
PpuMI RGGWCCY 1 cut(s) 1504
PscI ACATGT 1 cut(s) 2328
Psp124BI GAGCTC 3 cut(s) 1065, 1137, 2610
Psp5II RGGWCCY 1 cut(s) 1504
Psp6I CCWGG 7 cut(s) 674, 796, 828, 1873, 2275, 2360, 2631
PspCI CACGTG 1 cut(s) 1811
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 7 cut(s) 674, 796, 828, 1873, 2275, 2360, 2631
PspN4I GGNNCC 6 cut(s) 373, 562, 1505, 2484, 2618, 2638
PspPI GGNCC 7 cut(s) 49, 371, 800, 1113, 1504, 1703, 2629
PspPPI RGGWCCY 1 cut(s) 1504
PstI CTGCAG 2 cut(s) 1018, 2512
PstNI CAGNNNCTG 3 cut(s) 206, 2179, 2282
PsuI RGATCY 1 cut(s) 2542
RsaI GTAC 6 cut(s) 136, 648, 1037, 1943, 2450, 2461
RsaNI GTAC 6 cut(s) 135, 647, 1036, 1942, 2449, 2460
RseI CAYNNNNRTG 3 cut(s) 404, 1025, 2376
SacI GAGCTC 3 cut(s) 1065, 1137, 2610
SalI GTCGAC 2 cut(s) 569, 2048
SaqAI TTAA 7 cut(s) 509, 897, 924, 980, 1361, 2058, 2127
SatI GCNGC 8 cut(s) 519, 617, 624, 1559, 1902, 1950, 2508, 2511
Sau3AI GATC 6 cut(s) 786, 1279, 1880, 2152, 2157, 2542
Sau96I GGNCC 7 cut(s) 49, 371, 800, 1113, 1504, 1703, 2629
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 4 cut(s) 451, 665, 1318, 1714
ScrFI CCNGG 8 cut(s) 447, 676, 798, 830, 1875, 2277, 2362, 2633
SduI GDGCHC 6 cut(s) 1065, 1137, 1803, 2338, 2610, 2639
SfcI CTRYAG 2 cut(s) 1014, 2508
SfoI GGCGCC 1 cut(s) 2484
Sfr274I CTCGAG 1 cut(s) 1498
SinI GGWCC 4 cut(s) 49, 800, 1504, 2629
SlaI CTCGAG 1 cut(s) 1498
SmiMI CAYNNNNRTG 3 cut(s) 404, 1025, 2376
SmlI CTYRAG 3 cut(s) 1498, 1562, 1796
SmoI CTYRAG 3 cut(s) 1498, 1562, 1796
SsiI CCGC 4 cut(s) 847, 1299, 1450, 1904
SspDI GGCGCC 1 cut(s) 2482
SspI AATATT 1 cut(s) 2194
SspMI CTAG 6 cut(s) 15, 41, 126, 320, 644, 2613
SstI GAGCTC 3 cut(s) 1065, 1137, 2610
StyD4I CCNGG 8 cut(s) 445, 674, 796, 828, 1873, 2275, 2360, 2631
StyI CCWWGG 3 cut(s) 1706, 2309, 2612
TaiI ACGT 1 cut(s) 1813
TaqI TCGA 3 cut(s) 570, 1499, 2049
TatI WGTACW 2 cut(s) 1035, 2459
TfiI GAWTC 5 cut(s) 1627, 1843, 2117, 2227, 2428
Tru1I TTAA 7 cut(s) 509, 897, 924, 980, 1361, 2058, 2127
Tru9I TTAA 7 cut(s) 509, 897, 924, 980, 1361, 2058, 2127
TscAI CASTG 8 cut(s) 195, 946, 1055, 1381, 1583, 1732, 1754, 2010
TseFI GTSAC 3 cut(s) 184, 1243, 1727
TseI GCWGC 8 cut(s) 518, 616, 623, 1558, 1901, 1949, 2507, 2510
Tsp45I GTSAC 3 cut(s) 184, 1243, 1727
TspDTI ATGAA 8 cut(s) 218, 653, 713, 989, 1140, 2518, 2546, 2571
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 8 cut(s) 195, 946, 1055, 1381, 1583, 1732, 1754, 2010
Van91I CCANNNNNTGG 1 cut(s) 2179
VpaK11BI GGWCC 4 cut(s) 49, 800, 1504, 2629
XapI RAATTY 3 cut(s) 395, 920, 1780
XceI RCATGY 2 cut(s) 1299, 2332
XhoI CTCGAG 1 cut(s) 1498
XmaJI CCTAGG 1 cut(s) 2612
XmiI GTMKAC 4 cut(s) 362, 570, 1163, 2049
XmnI GAANNNNTTC 2 cut(s) 1626, 1780
XspI CTAG 6 cut(s) 15, 41, 126, 320, 644, 2613
ZrmI AGTACT 1 cut(s) 1037
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.