RchiOBHm_Chr2g0157981

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001253
Physical Location & Seq
Reverse (-)
74301984 .. 74309854
7871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52669

Sequence Viewer

Length: 2685 bp
ATGGTGGATGTAGCTGTTGCTGGTGCTGGGAAGGCAGGTGGGGAACCAGATTTGATCCCACCACCTCCTCCAACCATTCCGCCCAATGTGAAGCCAGAAGTCCTGAGTTCTCCCAAGTACTCTATTATGAGTAGGCGTGGTACTGGAACAACTGGGAGACGCATACAGTTGCTCGCAAACCACTTCAAAGTCACTGTGAAAGCTCCAGATGCTGTATTCTACCAGTATTCTGTTCATATTACTTCTGAAGACAAGAGGGCCGTTGAAGGCAAGGGAATTGGCAGAAAACTGGTTGAAAGGCTCTATCAAACATATTCTTCTGAACTTGCTGGTAAGAAGTTTGCGTATGATGGAGAGAAAGCTCTGTATACAGTTGGTCCTCTGCCACAGAACAAGCTTGAGTTTACAGTCGTGCTGGAGGAGACATTTGCAAAGAGTGAAAGTGGGAGTCCCAGTGAAGTGGCTGAAAAACGGTCAAGACGATCTCTTCAGTCAAAAACTTTTAACGTAGAGTTAAGTTATGCTGCCAAAATACCTCTGAAGTCAATTGCTCTTGCCCTCAAAGGAGCTGATGTCGACAATACTCAGGATGCATTGAGAGTGCTTGATATTATTCTACGGCAGCAAGCAGCTAACATGGGTTGCCTTCTGGTACGACAGTCATTCTTTCATGATGACTCCAGGAACTTTACTGATGTTGGGGCAGGTGTGATTGGTGTTCGGGGATTTCATTCCAGTTTCCGTCCAACTCAGGGTGGTTTGTCCTTGAATATGGATGTATCTACAACAATGATCATGACTCCTGGACCGGTAATAGATTTTCTGATTGCCAACCAGGGTGTGCGAGAACCCCGCAACATTGACTGGGTAAAGGCCAAGAGAATGCTGAAAAACATGAGGATTAAGACGAGACACCGTAACATGGAATTTAAAATAATAGGCATCAGCGAGAAGCCCTGCAATCTGCAATACTTTCCTATGAAGTTAAAGCCTGGTGATGGTGCAAGTGAAGGGGAGACTGCAGAGGTTACTGTGTTTGAGTATTTCACCAAACACTGTGGCATTGAACTCACTTACTCTGCATACTTGCCATGCCTTGATGTTGGCAAACCAAAAAGGCCCAACTATCTGCCTCTGGAGCTCTGTGCGCTTGTTTCCTTACAACGGTATACGAAAGCATTATCTTCACAGCAGAGGGCATCCTTAGTTGAAAAATCAAGGCAGAAGCCTCAGGATAGAATACGAACTGTGACTGATGCTGTATGCAAATACCAGTATGATAGTGATCCTGTGCTTACTACATGTGGTATTTCTATAGAGAAGCAATTAACTCAGTTTGAAGGGCGCGTTCTTGATACTCCGAAGTTAAAGGTAGGTAACAGTGAGGATTGCACCCCTTTTAAGGGACGATGGAACTTCAACAACAAGACACTTTTGAACCCCACTCGCATTGACCGCTGGATTGTTGTCAACTTCTCTGCACGCTGTGATACCAGCCATATCTCAAGGGAGCTTATCAACAGTGGGAGGAAAAAGGGCATTCTTATTGAGCGTCCCTATACGCTGCTTGAGGAAGAATCACAGTCTAGAAGACAAAGCCCTGTTATAAGAGTTGAAAAGATGTTTGAACAAATTCAAACTAAGCTTCCAAGTCCACCTGACTTCATTCTTTGTGTTTTACCAGAGAGGAAAAATTCTGATATTTATGGGCCTTGGAAGAAAAAGAGTCTCAGTGATTTCGGTATTGTCACACAATGCATTTCCCCTTCCAAGATTAATGATCAGTACCTTACCAATCTTGGAGGAATAAATTCTTTGTTGGCGCTAGAACATTCCTCACGTGTCCCTTTGATAAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGTCTCCTGGTCGATCAGACATTCCTTCTGTTGCTGCCGTTGTAGGCTCTCGAAGTTGGCCACTTATTTCAAGGTACAGAGCAGCTGTTAGAACACAATCTCCTAAGGTGGAAATGATTGATGCTCTTTACAAACCACTGGCAAATGGAAAAGATGATGGTATTATCAGGGAACTGCTTGTCGACTTCTATAAGACAAGCGGTGGACGTAAACCAACTCAGATTATTGTCTTCAGGGATGGAGTAAGTGAATCACAGTTTAATCAAGTCCTGAACATTGAGCTGGATCAAATTGTAAAGGCTTACCAGCACCTAGGGGAGGCTAATATTCCAAAGTTTACTGTGATTGTGGCTCAGAAGAATCATCACACAAAGCTATTCCAAACTAATTCCCCTGATAATGTTCCACCAGGGACTGTTGTTGACACCAAAATTGTGCATCCAAGGAATTATGATTTCTACATGTGTGCTCAGGCAGGCATGATTGGAACTTCCAGGCCAGCTCACTATCATGTCTTGCTTGATGAGATTGGCTTTTCCCCTGATGACTTGCAAAACCTGATCCACTCCCTTTCTTATGTGTACCAGAGAAGTACAACTGCAATCTCTATTGTGGCGCCCATATGCTACGCGCACCTTGCTGCAGCACAAGTGGGGCAGTTCATGAAGTTTGAAGATCTCTCGGACACCTCATCGGGGAAGGGCAGTGTCACTTCATCAGAGAGCATCCCAGTTCAAGAGCTTCCCAGGCTTCATGAGAAGGTCCAGGGCTCCATGTTCTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

894

Amino Acids

99.57

Weight (kDa)

9.2

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 53 - 206 5.3e-31 N-terminal domain of argonaute
ArgoL1 PF08699 218 - 265 2.5e-17 Argonaute linker 1 domain
PAZ PF02170 272 - 402 1.9e-29 PAZ domain
ArgoL2 PF16488 412 - 457 2.1e-09 Argonaute linker 2 domain
Piwi PF02171 555 - 855 4.2e-97 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
AarI CACCTGC 2 cut(s) 26, 695
Acc36I ACCTGC 2 cut(s) 26, 695
AccB1I GGYRCC 1 cut(s) 2512
AccI GTMKAC 4 cut(s) 368, 576, 1169, 2079
AccII CGCG 2 cut(s) 1347, 2528
AciI CCGC 4 cut(s) 80, 853, 1456, 2097
AclWI GGATC 4 cut(s) 49, 1280, 2190, 2452
AcoI YGGCCR 1 cut(s) 1955
AcsI RAATTY 4 cut(s) 926, 1632, 1693, 1810
AcuI CTGAAG 4 cut(s) 267, 473, 560, 2113
AcvI CACGTG 1 cut(s) 1841
AcyI GRCGYC 1 cut(s) 2513
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 7 cut(s) 119, 142, 654, 1787, 1973, 2480, 2491
AfiI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2215, 2592
AflIII ACRYGT 3 cut(s) 1301, 1840, 2358
AgeI ACCGGT 1 cut(s) 808
AjnI CCWGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
AloI GAACNNNNNNTCC 2 cut(s) 1981, 2013
Alw21I GWGCWC 2 cut(s) 1143, 2368
Alw26I GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1905
AlwI GGATC 4 cut(s) 49, 1280, 2190, 2452
AlwNI CAGNNNCTG 2 cut(s) 212, 2312
AoxI GGCC 6 cut(s) 258, 873, 1118, 1709, 1955, 2393
ApeKI GCWGC 8 cut(s) 524, 622, 629, 1564, 1931, 1979, 2537, 2540
ApoI RAATTY 4 cut(s) 926, 1632, 1693, 1810
AseI ATTAAT 1 cut(s) 1776
AsiGI ACCGGT 1 cut(s) 808
Asp700I GAANNNNTTC 2 cut(s) 1632, 1810
AspA2I CCTAGG 1 cut(s) 2209
AspLEI GCGC 5 cut(s) 1150, 1347, 1825, 2515, 2530
AspS9I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2659
AsuHPI GGTGA 2 cut(s) 1007, 1039
AvaII GGWCC 3 cut(s) 377, 806, 2659
AvrII CCTAGG 1 cut(s) 2209
AxyI CCTNAGG 2 cut(s) 1230, 2001
BaeI ACNNNNGTAYC 2 cut(s) 644, 677
BalI TGGCCA 1 cut(s) 1957
BanI GGYRCC 1 cut(s) 2512
BanII GRGCYC 2 cut(s) 1143, 2669
BbrPI CACGTG 1 cut(s) 1841
BbsI GAAGAC 3 cut(s) 255, 1597, 2119
Bbv12I GWGCWC 2 cut(s) 1143, 2368
BbvI GCAGC 8 cut(s) 511, 634, 641, 1551, 1918, 1991, 2524, 2552
BccI CCATC 6 cut(s) 344, 992, 1404, 1876, 2048, 2129
BceAI ACGGC 3 cut(s) 245, 635, 1919
BciT130I CCWGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
BclI TGATCA 2 cut(s) 792, 1780
BcoDI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1905
BfaI CTAG 3 cut(s) 1587, 1826, 2210
BfmI CTRYAG 3 cut(s) 1020, 1314, 2538
BfoI RGCGCY 2 cut(s) 1826, 2516
BfuAI ACCTGC 2 cut(s) 26, 695
BglII AGATCT 1 cut(s) 2572
BisI GCNGC 8 cut(s) 525, 623, 630, 1565, 1932, 1980, 2538, 2541
BlnI CCTAGG 1 cut(s) 2209
BlsI GCNGC 8 cut(s) 526, 624, 631, 1566, 1933, 1981, 2539, 2542
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
Bme18I GGWCC 3 cut(s) 377, 806, 2659
BmgT120I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2659
BmiI GGNNCC 3 cut(s) 45, 2514, 2668
BmrFI CCNGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
BmrI ACTGGG 4 cut(s) 162, 447, 874, 2621
BmsI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2008, 2344, 2631
BmuI ACTGGG 4 cut(s) 162, 447, 874, 2621
BpiI GAAGAC 3 cut(s) 255, 1597, 2119
BpmI CTGGAG 4 cut(s) 189, 437, 664, 1157
Bpu10I CCTNAGC 1 cut(s) 2367
BpuEI CTTGAG 3 cut(s) 419, 1489, 1589
BsaAI YACGTR 1 cut(s) 1841
BsaBI GATNNNNATC 1 cut(s) 1284
BsaHI GRCGYC 1 cut(s) 2513
BsaI GGTCTC 1 cut(s) 1905
BsaJI CCNNGG 7 cut(s) 835, 1712, 2209, 2306, 2339, 2642, 2662
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 6 cut(s) 439, 469, 558, 588, 1981, 2011
Bsc4I CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2215, 2592
Bse118I RCCGGY 1 cut(s) 808
Bse21I CCTNAGG 2 cut(s) 1230, 2001
Bse8I GATNNNNATC 1 cut(s) 1284
BseBI CCWGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
BseDI CCNNGG 7 cut(s) 835, 1712, 2209, 2306, 2339, 2642, 2662
BseGI GGATG 9 cut(s) 13, 595, 781, 1199, 1887, 1891, 2140, 2335, 2622
BseJI GATNNNNATC 1 cut(s) 1284
BseLI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2215, 2592
BseMII CTCAG 9 cut(s) 95, 599, 764, 1244, 1346, 1744, 2129, 2264, 2381
BseRI GAGGAG 2 cut(s) 57, 434
BseXI GCAGC 8 cut(s) 511, 634, 641, 1551, 1918, 1991, 2524, 2552
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 1464
Bsh1236I CGCG 2 cut(s) 1347, 2528
BshFI GGCC 6 cut(s) 260, 875, 1120, 1711, 1957, 2395
BshNI GGYRCC 1 cut(s) 2512
BshTI ACCGGT 1 cut(s) 808
BsiHKAI GWGCWC 2 cut(s) 1143, 2368
BsiSI CCGG 1 cut(s) 809
BslFI GGGAC 5 cut(s) 435, 1419, 1539, 1829, 2323
BslI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2215, 2592
BsmAI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1905
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 5 cut(s) 435, 1419, 1539, 1829, 2323
BsmI GAATGC 2 cut(s) 888, 1539
BsnI GGCC 6 cut(s) 260, 875, 1120, 1711, 1957, 2395
Bso31I GGTCTC 1 cut(s) 1905
Bsp1286I GDGCHC 3 cut(s) 1143, 2368, 2669
Bsp143I GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
BspACI CCGC 4 cut(s) 80, 853, 1456, 2097
BspANI GGCC 6 cut(s) 260, 875, 1120, 1711, 1957, 2395
BspCNI CTCAG 9 cut(s) 96, 598, 763, 1243, 1345, 1743, 2128, 2263, 2380
BspFNI CGCG 2 cut(s) 1347, 2528
BspHI TCATGA 4 cut(s) 670, 795, 2559, 2650
BspLI GGNNCC 3 cut(s) 45, 2514, 2668
BspMAI CTGCAG 2 cut(s) 1024, 2542
BspMI ACCTGC 2 cut(s) 26, 695
BspPI GGATC 4 cut(s) 49, 1280, 2190, 2452
BspT107I GGYRCC 1 cut(s) 2512
BspTNI GGTCTC 1 cut(s) 1905
BsrFI RCCGGY 1 cut(s) 808
BssAI RCCGGY 1 cut(s) 808
BssECI CCNNGG 7 cut(s) 835, 1712, 2209, 2306, 2339, 2642, 2662
BssMI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
BssNAI GTATAC 2 cut(s) 369, 1170
BssNI GRCGYC 1 cut(s) 2513
BssT1I CCWWGG 3 cut(s) 1712, 2209, 2339
Bst1107I GTATAC 2 cut(s) 369, 1170
Bst2UI CCWGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 2513
BstBAI YACGTR 1 cut(s) 1841
BstC8I GCNNGC 5 cut(s) 174, 627, 1483, 2374, 2397
BstENI CCTNNNNNAGG 1 cut(s) 2213
BstF5I GGATG 9 cut(s) 13, 595, 781, 1199, 1887, 1891, 2140, 2335, 2622
BstFNI CGCG 2 cut(s) 1347, 2528
BstH2I RGCGCY 2 cut(s) 1826, 2516
BstHHI GCGC 5 cut(s) 1150, 1347, 1825, 2515, 2530
BstKTI GATC 9 cut(s) 57, 485, 795, 1288, 1783, 1913, 2185, 2460, 2575
BstMAI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1905
BstMBI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
BstMWI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2534, 2644
BstNI CCWGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
BstNSI RCATGY 2 cut(s) 1305, 2362
BstSCI CCNGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
BstSFI CTRYAG 3 cut(s) 1020, 1314, 2538
BstUI CGCG 2 cut(s) 1347, 2528
BstV1I GCAGC 8 cut(s) 511, 634, 641, 1551, 1918, 1991, 2524, 2552
BstV2I GAAGAC 3 cut(s) 255, 1597, 2119
BstX2I RGATCY 1 cut(s) 2572
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 2572
BstZ17I GTATAC 2 cut(s) 369, 1170
Bsu36I CCTNAGG 2 cut(s) 1230, 2001
BsuRI GGCC 6 cut(s) 260, 875, 1120, 1711, 1957, 2395
BtsCI GGATG 9 cut(s) 13, 595, 781, 1199, 1887, 1891, 2140, 2335, 2622
BtsI GCAGTG 1 cut(s) 2608
BtsIMutI CAGTG 8 cut(s) 192, 460, 1054, 1387, 1528, 1738, 2033, 2608
BveI ACCTGC 2 cut(s) 26, 695
Cac8I GCNNGC 5 cut(s) 174, 627, 1483, 2374, 2397
CaiI CAGNNNCTG 2 cut(s) 212, 2312
CciI TCATGA 4 cut(s) 670, 795, 2559, 2650
CfoI GCGC 5 cut(s) 1150, 1347, 1825, 2515, 2530
Cfr10I RCCGGY 1 cut(s) 808
Cfr13I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2659
CseI GACGC 2 cut(s) 168, 1541
Csp6I GTAC 7 cut(s) 118, 141, 653, 1786, 1972, 2479, 2490
CspAI ACCGGT 1 cut(s) 808
CspCI CAANNNNNGTGG 4 cut(s) 1039, 1074, 1432, 1467
CviQI GTAC 7 cut(s) 118, 141, 653, 1786, 1972, 2479, 2490
DinI GGCGCC 1 cut(s) 2514
DpnI GATC 9 cut(s) 56, 484, 794, 1287, 1782, 1912, 2184, 2459, 2574
DpnII GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
DraI TTTAAA 1 cut(s) 931
DraIII CACNNNGTG 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 1955
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 1141
Eco130I CCWWGG 3 cut(s) 1712, 2209, 2339
Eco24I GRGCYC 2 cut(s) 1143, 2669
Eco31I GGTCTC 1 cut(s) 1905
Eco47I GGWCC 3 cut(s) 377, 806, 2659
Eco53kI GAGCTC 1 cut(s) 1141
Eco57I CTGAAG 4 cut(s) 267, 473, 560, 2113
Eco72I CACGTG 1 cut(s) 1841
Eco81I CCTNAGG 2 cut(s) 1230, 2001
EcoICRI GAGCTC 1 cut(s) 1141
EcoNI CCTNNNNNAGG 1 cut(s) 2213
EcoRII CCWGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
EcoT14I CCWWGG 3 cut(s) 1712, 2209, 2339
EcoT22I ATGCAT 2 cut(s) 595, 1760
EcoT38I GRGCYC 2 cut(s) 1143, 2669
EgeI GGCGCC 1 cut(s) 2514
EheI GGCGCC 1 cut(s) 2514
ErhI CCWWGG 3 cut(s) 1712, 2209, 2339
Esp3I CGTCTC 1 cut(s) 151
FaqI GGGAC 5 cut(s) 435, 1419, 1539, 1829, 2323
FauI CCCGC 1 cut(s) 860
FauNDI CATATG 1 cut(s) 2519
FbaI TGATCA 2 cut(s) 792, 1780
FblI GTMKAC 4 cut(s) 368, 576, 1169, 2079
Fnu4HI GCNGC 8 cut(s) 525, 623, 630, 1565, 1932, 1980, 2538, 2541
FokI GGATG 9 cut(s) 20, 602, 788, 1186, 1894, 1898, 2147, 2322, 2609
FriOI GRGCYC 2 cut(s) 1143, 2669
Fsp4HI GCNGC 8 cut(s) 525, 623, 630, 1565, 1932, 1980, 2538, 2541
FspBI CTAG 3 cut(s) 1587, 1826, 2210
GlaI GCGC 5 cut(s) 1149, 1346, 1824, 2514, 2529
GluI GCNGC 8 cut(s) 525, 623, 630, 1565, 1932, 1980, 2538, 2541
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 4 cut(s) 189, 437, 664, 1157
HaeII RGCGCY 2 cut(s) 1826, 2516
HaeIII GGCC 6 cut(s) 260, 875, 1120, 1711, 1957, 2395
HapII CCGG 1 cut(s) 809
HgaI GACGC 2 cut(s) 168, 1541
HhaI GCGC 5 cut(s) 1150, 1347, 1825, 2515, 2530
Hin1I GRCGYC 1 cut(s) 2513
Hin6I GCGC 5 cut(s) 1148, 1345, 1823, 2513, 2528
HinP1I GCGC 5 cut(s) 1148, 1345, 1823, 2513, 2528
HincII GTYRAC 4 cut(s) 577, 1471, 2080, 2320
HindII GTYRAC 4 cut(s) 577, 1471, 2080, 2320
HindIII AAGCTT 2 cut(s) 395, 1643
HinfI GANTC 7 cut(s) 448, 677, 799, 1577, 1726, 2147, 2257
HpaII CCGG 1 cut(s) 809
HphI GGTGA 2 cut(s) 1007, 1039
HpyAV CCTTC 9 cut(s) 25, 260, 656, 1004, 1334, 1776, 1932, 2590, 2650
HpyCH4IV ACGT 3 cut(s) 507, 1840, 2104
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2534, 2644
HpySE526I ACGT 3 cut(s) 507, 1840, 2104
Hsp92I GRCGYC 1 cut(s) 2513
HspAI GCGC 5 cut(s) 1148, 1345, 1823, 2513, 2528
KasI GGCGCC 1 cut(s) 2512
Ksp22I TGATCA 2 cut(s) 792, 1780
Kzo9I GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
LmnI GCTCC 5 cut(s) 208, 566, 1138, 1510, 2672
Lsp1109I GCAGC 8 cut(s) 511, 634, 641, 1551, 1918, 1991, 2524, 2552
LweI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2008, 2344, 2631
MaeI CTAG 3 cut(s) 1587, 1826, 2210
MaeII ACGT 3 cut(s) 507, 1840, 2104
MaeIII GTNAC 7 cut(s) 190, 917, 1027, 1249, 1376, 1747, 2605
MalI GATC 9 cut(s) 56, 484, 794, 1287, 1782, 1912, 2184, 2459, 2574
MboI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 2572
MhlI GDGCHC 3 cut(s) 1143, 2368, 2669
MlsI TGGCCA 1 cut(s) 1957
MluNI TGGCCA 1 cut(s) 1957
Mly113I GGCGCC 1 cut(s) 2513
MlyI GAGTC 4 cut(s) 457, 671, 793, 1735
MmeI TCCRAC 2 cut(s) 95, 770
Mox20I TGGCCA 1 cut(s) 1957
Mph1103I ATGCAT 2 cut(s) 595, 1760
MroXI GAANNNNTTC 2 cut(s) 1632, 1810
MscI TGGCCA 1 cut(s) 1957
MslI CAYNNNNRTG 1 cut(s) 2406
Msp20I TGGCCA 1 cut(s) 1957
MspA1I CMGCKG 2 cut(s) 1458, 1982
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 2 cut(s) 888, 1539
MvaI CCWGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
MvnI CGCG 2 cut(s) 1347, 2528
MwoI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2534, 2644
NarI GGCGCC 1 cut(s) 2513
NdeI CATATG 1 cut(s) 2519
NdeII GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
NlaIV GGNNCC 3 cut(s) 45, 2514, 2668
NmuCI GTSAC 4 cut(s) 190, 1249, 1747, 2605
NsiI ATGCAT 2 cut(s) 595, 1760
NspI RCATGY 2 cut(s) 1305, 2362
PagI TCATGA 4 cut(s) 670, 795, 2559, 2650
PaqCI CACCTGC 2 cut(s) 26, 695
PciI ACATGT 2 cut(s) 1301, 2358
PcsI WCGNNNNNNNCGW 1 cut(s) 478
PctI GAATGC 2 cut(s) 888, 1539
PdmI GAANNNNTTC 2 cut(s) 1632, 1810
PfeI GAWTC 3 cut(s) 1577, 2147, 2257
PfoI TCCNGGA 2 cut(s) 680, 802
PinAI ACCGGT 1 cut(s) 808
PkrI GCNGC 8 cut(s) 526, 624, 631, 1566, 1933, 1981, 2539, 2542
PleI GAGTC 4 cut(s) 456, 671, 793, 1734
PluTI GGCGCC 1 cut(s) 2516
PmaCI CACGTG 1 cut(s) 1841
PmlI CACGTG 1 cut(s) 1841
PpsI GAGTC 4 cut(s) 456, 671, 793, 1734
Ppu21I YACGTR 1 cut(s) 1841
PscI ACATGT 2 cut(s) 1301, 2358
PshBI ATTAAT 1 cut(s) 1776
PsiI TTATAA 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 1143
Psp6I CCWGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
PspCI CACGTG 1 cut(s) 1841
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
PspN4I GGNNCC 3 cut(s) 45, 2514, 2668
PspPI GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2659
PstI CTGCAG 2 cut(s) 1024, 2542
PstNI CAGNNNCTG 2 cut(s) 212, 2312
PsuI RGATCY 1 cut(s) 2572
PvuII CAGCTG 1 cut(s) 1982
RsaI GTAC 7 cut(s) 119, 142, 654, 1787, 1973, 2480, 2491
RsaNI GTAC 7 cut(s) 118, 141, 653, 1786, 1972, 2479, 2490
RseI CAYNNNNRTG 1 cut(s) 2406
SacI GAGCTC 1 cut(s) 1143
SalI GTCGAC 2 cut(s) 575, 2078
SatI GCNGC 8 cut(s) 525, 623, 630, 1565, 1932, 1980, 2538, 2541
Sau3AI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1910, 2182, 2457, 2572
Sau96I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2659
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 4 cut(s) 457, 671, 793, 1735
ScrFI CCNGG 9 cut(s) 682, 804, 836, 993, 1905, 2307, 2392, 2644, 2663
SduI GDGCHC 3 cut(s) 1143, 2368, 2669
SfaNI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2008, 2344, 2631
SfcI CTRYAG 3 cut(s) 1020, 1314, 2538
SfoI GGCGCC 1 cut(s) 2514
SinI GGWCC 3 cut(s) 377, 806, 2659
SmiMI CAYNNNNRTG 1 cut(s) 2406
SmlI CTYRAG 3 cut(s) 398, 1504, 1568
SmoI CTYRAG 3 cut(s) 398, 1504, 1568
SsiI CCGC 4 cut(s) 80, 853, 1456, 2097
SspDI GGCGCC 1 cut(s) 2512
SspI AATATT 1 cut(s) 2224
SspMI CTAG 3 cut(s) 1587, 1826, 2210
SstI GAGCTC 1 cut(s) 1143
StyD4I CCNGG 9 cut(s) 680, 802, 834, 991, 1903, 2305, 2390, 2642, 2661
StyI CCWWGG 3 cut(s) 1712, 2209, 2339
TaiI ACGT 3 cut(s) 510, 1843, 2107
TaqI TCGA 4 cut(s) 576, 1909, 1948, 2079
TatI WGTACW 2 cut(s) 117, 2489
TfiI GAWTC 3 cut(s) 1577, 2147, 2257
TscAI CASTG 8 cut(s) 199, 460, 1061, 1387, 1528, 1738, 2040, 2608
TseFI GTSAC 4 cut(s) 190, 1249, 1747, 2605
TseI GCWGC 8 cut(s) 524, 622, 629, 1564, 1931, 1979, 2537, 2540
Tsp45I GTSAC 4 cut(s) 190, 1249, 1747, 2605
TspDTI ATGAA 9 cut(s) 224, 659, 719, 995, 1654, 2548, 2576, 2601, 2639
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 8 cut(s) 199, 460, 1061, 1387, 1528, 1738, 2040, 2608
VpaK11BI GGWCC 3 cut(s) 377, 806, 2659
VspI ATTAAT 1 cut(s) 1776
XagI CCTNNNNNAGG 1 cut(s) 2213
XapI RAATTY 4 cut(s) 926, 1632, 1693, 1810
XbaI TCTAGA 1 cut(s) 1586
XceI RCATGY 2 cut(s) 1305, 2362
XmaJI CCTAGG 1 cut(s) 2209
XmiI GTMKAC 4 cut(s) 368, 576, 1169, 2079
XmnI GAANNNNTTC 2 cut(s) 1632, 1810
XspI CTAG 3 cut(s) 1587, 1826, 2210
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 595, 1760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.