Rh2AG529900

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001253
Physical Location & Seq
Reverse (-)
75872938 .. 75879790
6853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG529900.1

Sequence Viewer

Length: 2634 bp
ATGATTGATTTTCTGTACAAGCCACTGGAAAGTACAGAAGATGATGGTACTATGAGGGAACTGCTTAAGGACTTCTATGAAACTAGCAGATGCAAACCAACTCAGATTATTATCTTCAGGGATGGAGTCAGCGAATCGCAATTTAATCAAGTTTTGAATGAGGAGCTGGATCAAATTATGAATGCTTACAAGCACCACCAGAAGGTTGATGAGATTCCAAAATTTAATGTGCTTGTGGCTCAAAAGAACCATAATACAAAGCTATTCCAAGCTGTTTTTCCCCAGCATAATGTTCCACCAGGAACAGTTGTGGACACAAATATTGTGCATCCAAGGAATTATGATTTCTACATGTGTGCTCACAAAGGCTCCATTGATCTCTCGGAAACTTCATCATCAGGAAGGGACAGTGTCACTACATCACAGAGTATCCCAGTGCCTGAGCTCCCTAGGCTTGATGGGAAGGTCCACGGTTCCATTGAAACTGGCAGCTCTAATGTCACTGAAAAACAGATGAAACGATCTATTCAGTCAAAGACTTTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAGCCAATTGCTCTTGCCCTTAAAGGAGCTGATGCAGATGATGGTCAAGATGCATTGAGAGTGCTCGACATAGTCTTAAGGCAGCAAGCAGCTAACAGGGGATGTCTTCTAGTAAGACAGTCATTCCTTCATGATGACTCCAGATGCATCACTGATGTTGGAGGTACCGGTTTAAGTACTGTTCGGGGCTTCCATTCCAGTTTTCGTCCCACTCAGGGTGGCCTGACCTTGAACATGGATGTATCTACTACACTGATCTTAACCCCTGGACAAGTGATTGATTTTCTGAAAGCTAACCAGGGTGTACAACACCCCCGCAACATTGACTGGGCAAGGTCTAAGAGAATGCTGAAAAATATGAGGATAAAGACAAGACATCACAACATGGAATTCAAAATTACTGGCTTGAGCAAGAATCCCTGCCAACTTACCTATTCTGCATACTTTCCATGTCTTGATGTTGGCAAGCCAAAAAGGCCTAACTATGTGCCGCTCGAGCTTTGTTCCCTTGTTTCCCTCCAACGGTATAAAAAAGCATTATCTTCAAAGCAGAGAGCTTCTATAGTTGGAAGTTCAAGGCAGAGGCCTCAGGAGAGAATAAACAGTGTGACTAATGCTGTAAGGAATTATGGCTATAATGATGATCCTGTGCTTGCTGCATGCGGTATATCCATAGAGAAGCAATTGGCTCAGATTGAAGGTCGTGTACTTGAGGCTCCGAAGTTGAAGGTAGGCAACAGTTCCGATTGCATGCCTTCAGATGGGAGATGGAACTTCAATGGCAAGACATTTTTGAACCCCAAACACATTGACAAATGGATTGTTGTCAACTTCTCTTTGGAAAATTGTGATACTAGTCGCATCTCAAGGCAGCTTTATGACATTGGGAGGAAGAAGGGCATTAGAATTGAGTGCCCTATCACATTGATTGAGGAACAACCACAGCTTAAAAAACAAAGCCCTATTGAAAGAGTTAAAAAGATGTTTGAACAGATTAAAGTGAAGCATCCTAAAGAGCCACCAAACTTCATTCTTTGTGTTTTACCAGAAAGAAAAACTTCTGATATATATGGACCGTGGAAGAAAGAGTGCCTTTACCTCACTAATGTACTTTTGAAAATCAATTCTAAGCTTGGAGGAATAAATTCTTTGTTGGCAACAGAACACTCTGGGGTTGTTTCTCTAATTAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGCCTCCTGGTCGATCAGATATTCCTTCTGTTGCTGCGGTTGTTGGCTCTCTACAGTGGCCACTAATTTCACAGTACAGAGTGGCTGTAAGGACACAAAAAGTTTGTAAGGAGATGATCGATTCTCTGTACAAGCCACTGGAAAGTGGAGAAGATGATGGTATTATGAGGGAACTGCTTAAGGAATTCTATGAAACTAGCAATGCAAACCAACTCAGATTATTATCTTCAGGTGTGTATTATGGCATTGTGTTATTTAACATGGATGGAGTCAGCGAATCGCAATTTAATCAAGTTTTGAATGAGGAGCTGGATCAAATTATGAAGGCTTACAAGCACCACCAGAAGGTTGATGAGCTTCCAAAATTTACTGTGATTGTGGCTCAAAAGAACCATAATACAAAGCTATTCCAAGCTGGTTCTCCCCAGTATAATGTTCCACCAGGAACAGTTGTGGATACAAATATTGTGCATCCAAGGAATTATGATTTCTATATGTGTGCTCACAAAGGCTCCATTGGAACTTCTAGGCCAGCACACTGTCATGTCTTGCTTGATGAGATTGGCTTTTCTGCCGATGAACTGCAAAACCTGATCCACTCTCTTTCCTATGTGTACCAGAGGAGTACAACTGCAGTCTCAATTGTTGCACCCGTATGTTATGCGCACCTTGCTGCAGCACAAGTAATGCAGTTTATGAAGTTTGAAGATCTCTGGGAAACTTCATCATCAGGAAGGGTCAGTGTCACTACATCAGAGAGTATCCCAGTGCCTGAGCTCCCTAGGCTTCATGAGAAGGTCCGCGGTTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

877

Amino Acids

98.6

Weight (kDa)

8.84

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 15 - 126 5e-34 Piwi domain
ArgoL1 PF08699 231 - 279 8.6e-14 Argonaute linker 1 domain
PAZ PF02170 336 - 381 4.4e-08 PAZ domain
ArgoL2 PF16488 391 - 436 3.4e-14 Argonaute linker 2 domain
ArgoMid PF16487 444 - 498 1.1e-06 Mid domain of argonaute
Piwi PF02171 558 - 837 8.1e-81 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2475
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 1072
AccII CGCG 1 cut(s) 2613
AciI CCGC 6 cut(s) 895, 1070, 1242, 1847, 2611, 2613
AclWI GGATC 4 cut(s) 177, 1217, 2130, 2398
AcoI YGGCCR 1 cut(s) 1868
AcsI RAATTY 5 cut(s) 221, 968, 1723, 1994, 2174
AcuI CTGAAG 4 cut(s) 100, 599, 1320, 2022
AfiI CCNNNNNNNGG 5 cut(s) 202, 794, 1101, 1340, 2155
AflII CTTAAG 3 cut(s) 65, 655, 1988
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 746
AhlI ACTAGT 1 cut(s) 1433
AjnI CCWGG 5 cut(s) 298, 844, 876, 1816, 2251
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
Alw21I GWGCWC 5 cut(s) 361, 447, 645, 2314, 2589
Alw26I GTCTC 1 cut(s) 2452
AlwI GGATC 4 cut(s) 177, 1217, 2130, 2398
AlwNI CAGNNNCTG 2 cut(s) 440, 2582
Ama87I CYCGRG 1 cut(s) 1073
AoxI GGCC 6 cut(s) 799, 1055, 1163, 1811, 1868, 2339
ApeKI GCWGC 9 cut(s) 489, 563, 661, 668, 1235, 1450, 1844, 2483, 2486
ApoI RAATTY 5 cut(s) 221, 968, 1723, 1994, 2174
AsiGI ACCGGT 1 cut(s) 746
Asp700I GAANNNNTTC 2 cut(s) 1636, 1723
Asp718I GGTACC 1 cut(s) 743
AspA2I CCTAGG 2 cut(s) 449, 2591
AspLEI GCGC 1 cut(s) 2476
AspS9I GGNCC 4 cut(s) 466, 1652, 1811, 2608
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 3 cut(s) 466, 1652, 2608
AvrII CCTAGG 2 cut(s) 449, 2591
AxyI CCTNAGG 1 cut(s) 1167
BaeGI GKGCMC 1 cut(s) 1496
BalI TGGCCA 1 cut(s) 1870
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 2 cut(s) 447, 2589
BarI GAAGNNNNNNTAC 2 cut(s) 1269, 1301
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 5 cut(s) 361, 447, 645, 2314, 2589
BbvI GCAGC 9 cut(s) 501, 550, 673, 680, 1222, 1462, 1831, 2470, 2498
BccI CCATC 9 cut(s) 38, 116, 452, 614, 1334, 1341, 1789, 1961, 2069
BciT130I CCWGG 5 cut(s) 300, 846, 878, 1818, 2253
BciVI GTATCC 3 cut(s) 440, 2260, 2582
BcoDI GTCTC 1 cut(s) 2452
BcuI ACTAGT 1 cut(s) 1433
BfaI CTAG 7 cut(s) 84, 450, 689, 1434, 2007, 2337, 2592
BfmI CTRYAG 4 cut(s) 1140, 1862, 2442, 2484
BfrI CTTAAG 3 cut(s) 65, 655, 1988
BfuI GTATCC 3 cut(s) 440, 2260, 2582
BglI GCCNNNNNGGC 1 cut(s) 1054
BglII AGATCT 1 cut(s) 2518
BlnI CCTAGG 2 cut(s) 449, 2591
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 5 cut(s) 300, 846, 878, 1818, 2253
Bme18I GGWCC 3 cut(s) 466, 1652, 2608
BmeT110I CYCGRG 1 cut(s) 1073
BmgT120I GGNCC 4 cut(s) 466, 1652, 1811, 2608
BmiI GGNNCC 6 cut(s) 370, 475, 745, 1296, 2323, 2617
BmrFI CCNGG 5 cut(s) 300, 846, 878, 1818, 2253
BmrI ACTGGG 4 cut(s) 428, 916, 2230, 2570
BmsI GCATC 9 cut(s) 80, 337, 601, 619, 713, 735, 1449, 1594, 2290
BmuI ACTGGG 4 cut(s) 428, 916, 2230, 2570
BpiI GAAGAC 1 cut(s) 677
BpmI CTGGAG 1 cut(s) 703
Bpu10I CCTNAGC 2 cut(s) 441, 2583
BpuEI CTTGAG 3 cut(s) 1006, 1310, 1429
Bsa29I ATCGAT 1 cut(s) 1929
BsaBI GATNNNNATC 2 cut(s) 110, 2032
BsaJI CCNNGG 9 cut(s) 332, 449, 469, 844, 877, 1655, 2285, 2591, 2611
BsaWI WCCGGW 1 cut(s) 746
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 2306, 2336
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 794, 1101, 1340, 2155
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 9 cut(s) 30, 434, 490, 777, 911, 985, 1953, 2236, 2576
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 1 cut(s) 2017
Bse8I GATNNNNATC 2 cut(s) 110, 2032
BseBI CCWGG 5 cut(s) 300, 846, 878, 1818, 2253
BseCI ATCGAT 1 cut(s) 1929
BseDI CCNNGG 9 cut(s) 332, 449, 469, 844, 877, 1655, 2285, 2591, 2611
BseGI GGATG 9 cut(s) 127, 328, 686, 823, 1585, 1800, 1804, 2080, 2281
BseJI GATNNNNATC 2 cut(s) 110, 2032
BseLI CCNNNNNNNGG 5 cut(s) 202, 794, 1101, 1340, 2155
BseMI GCAATG 1 cut(s) 2017
BseMII CTCAG 7 cut(s) 116, 432, 806, 1181, 1283, 2038, 2574
BseNI ACTGG 9 cut(s) 30, 434, 490, 777, 911, 985, 1953, 2236, 2576
BseRI GAGGAG 3 cut(s) 176, 2129, 2446
BseSI GKGCMC 1 cut(s) 1496
BseXI GCAGC 9 cut(s) 501, 550, 673, 680, 1222, 1462, 1831, 2470, 2498
BseYI CCCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 2613
BshFI GGCC 6 cut(s) 801, 1057, 1165, 1813, 1870, 2341
BshNI GGYRCC 1 cut(s) 743
BshTI ACCGGT 1 cut(s) 746
BshVI ATCGAT 1 cut(s) 1929
BsiHKAI GWGCWC 5 cut(s) 361, 447, 645, 2314, 2589
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 747
BslFI GGGAC 2 cut(s) 419, 771
BslI CCNNNNNNNGG 5 cut(s) 202, 794, 1101, 1340, 2155
BsmAI GTCTC 1 cut(s) 2452
BsmFI GGGAC 2 cut(s) 419, 771
BsmI GAATGC 2 cut(s) 187, 930
BsnI GGCC 6 cut(s) 801, 1057, 1165, 1813, 1870, 2341
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 6 cut(s) 361, 447, 645, 1496, 2314, 2589
Bsp1407I TGTACA 3 cut(s) 15, 883, 1938
BspACI CCGC 6 cut(s) 895, 1070, 1242, 1847, 2611, 2613
BspANI GGCC 6 cut(s) 801, 1057, 1165, 1813, 1870, 2341
BspCNI CTCAG 7 cut(s) 115, 433, 805, 1180, 1282, 2037, 2575
BspDI ATCGAT 1 cut(s) 1929
BspFNI CGCG 1 cut(s) 2613
BspHI TCATGA 2 cut(s) 709, 2599
BspLI GGNNCC 6 cut(s) 370, 475, 745, 1296, 2323, 2617
BspMAI CTGCAG 2 cut(s) 2446, 2488
BspPI GGATC 4 cut(s) 177, 1217, 2130, 2398
BspT107I GGYRCC 1 cut(s) 743
BspTI CTTAAG 3 cut(s) 65, 655, 1988
BsrBI CCGCTC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 2017
BsrFI RCCGGY 1 cut(s) 746
BsrGI TGTACA 3 cut(s) 15, 883, 1938
BsrI ACTGG 9 cut(s) 30, 434, 490, 777, 911, 985, 1953, 2236, 2576
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 9 cut(s) 332, 449, 469, 844, 877, 1655, 2285, 2591, 2611
BssT1I CCWWGG 4 cut(s) 332, 449, 2285, 2591
Bst2UI CCWGG 5 cut(s) 300, 846, 878, 1818, 2253
BstAFI CTTAAG 3 cut(s) 65, 655, 1988
BstAUI TGTACA 3 cut(s) 15, 883, 1938
BstC8I GCNNGC 6 cut(s) 666, 1046, 1233, 1240, 1331, 2343
BstDEI CTNAG 9 cut(s) 102, 441, 792, 918, 1167, 1269, 1707, 2024, 2583
BstDSI CCRYGG 3 cut(s) 469, 1655, 2611
BstF5I GGATG 9 cut(s) 127, 328, 686, 823, 1585, 1800, 1804, 2080, 2281
BstFNI CGCG 1 cut(s) 2613
BstHHI GCGC 1 cut(s) 2476
BstMAI GTCTC 1 cut(s) 2452
BstMWI GCNNNNNNNGC 5 cut(s) 451, 1054, 1075, 2480, 2593
BstNI CCWGG 5 cut(s) 300, 846, 878, 1818, 2253
BstNSI RCATGY 3 cut(s) 355, 1242, 1333
BstSCI CCNGG 5 cut(s) 298, 844, 876, 1816, 2251
BstSFI CTRYAG 4 cut(s) 1140, 1862, 2442, 2484
BstSLI GKGCMC 1 cut(s) 1496
BstUI CGCG 1 cut(s) 2613
BstV1I GCAGC 9 cut(s) 501, 550, 673, 680, 1222, 1462, 1831, 2470, 2498
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 1 cut(s) 2518
BstYI RGATCY 1 cut(s) 2518
Bsu15I ATCGAT 1 cut(s) 1929
Bsu36I CCTNAGG 1 cut(s) 1167
BsuI GTATCC 3 cut(s) 440, 2260, 2582
BsuRI GGCC 6 cut(s) 801, 1057, 1165, 1813, 1870, 2341
BsuTUI ATCGAT 1 cut(s) 1929
BtgI CCRYGG 3 cut(s) 469, 1655, 2611
BtsCI GGATG 9 cut(s) 127, 328, 686, 823, 1585, 1800, 1804, 2080, 2281
Cac8I GCNNGC 6 cut(s) 666, 1046, 1233, 1240, 1331, 2343
CaiI CAGNNNCTG 2 cut(s) 440, 2582
CciI TCATGA 2 cut(s) 709, 2599
CfoI GCGC 1 cut(s) 2476
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 4 cut(s) 466, 1652, 1811, 2608
Cfr42I CCGCGG 1 cut(s) 2614
ClaI ATCGAT 1 cut(s) 1929
CspAI ACCGGT 1 cut(s) 746
DdeI CTNAG 9 cut(s) 102, 441, 792, 918, 1167, 1269, 1707, 2024, 2583
EaeI YGGCCR 1 cut(s) 1868
Ecl136II GAGCTC 2 cut(s) 445, 2587
Eco130I CCWWGG 4 cut(s) 332, 449, 2285, 2591
Eco147I AGGCCT 2 cut(s) 1057, 1165
Eco24I GRGCYC 2 cut(s) 447, 2589
Eco47I GGWCC 3 cut(s) 466, 1652, 2608
Eco53kI GAGCTC 2 cut(s) 445, 2587
Eco57I CTGAAG 4 cut(s) 100, 599, 1320, 2022
Eco81I CCTNAGG 1 cut(s) 1167
Eco88I CYCGRG 1 cut(s) 1073
EcoICRI GAGCTC 2 cut(s) 445, 2587
EcoRI GAATTC 2 cut(s) 968, 1994
EcoRII CCWGG 5 cut(s) 298, 844, 876, 1816, 2251
EcoT14I CCWWGG 4 cut(s) 332, 449, 2285, 2591
EcoT22I ATGCAT 2 cut(s) 634, 728
EcoT38I GRGCYC 2 cut(s) 447, 2589
ErhI CCWWGG 4 cut(s) 332, 449, 2285, 2591
FalI AAGNNNNNCTT 4 cut(s) 1621, 1653, 1656, 1688
FaqI GGGAC 2 cut(s) 419, 771
FauI CCCGC 1 cut(s) 902
FokI GGATG 9 cut(s) 134, 315, 693, 830, 1572, 1807, 1811, 2087, 2268
FriOI GRGCYC 2 cut(s) 447, 2589
FspAI RTGCGCAY 1 cut(s) 2475
FspBI CTAG 7 cut(s) 84, 450, 689, 1434, 2007, 2337, 2592
FspI TGCGCA 1 cut(s) 2475
GlaI GCGC 1 cut(s) 2475
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 703
HaeIII GGCC 6 cut(s) 801, 1057, 1165, 1813, 1870, 2341
HapII CCGG 1 cut(s) 747
HhaI GCGC 1 cut(s) 2476
Hin6I GCGC 1 cut(s) 2474
HinP1I GCGC 1 cut(s) 2474
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HindIII AAGCTT 1 cut(s) 1709
HinfI GANTC 8 cut(s) 126, 134, 214, 716, 994, 1931, 2079, 2087
HpaII CCGG 1 cut(s) 747
Hpy166II GTNNAC 6 cut(s) 313, 469, 884, 1286, 1408, 2425
Hpy188III TCNNGA 8 cut(s) 399, 626, 710, 720, 1034, 1169, 2541, 2600
Hpy8I GTNNAC 6 cut(s) 313, 469, 884, 1286, 1408, 2425
HpyF10VI GCNNNNNNNGC 5 cut(s) 451, 1054, 1075, 2480, 2593
HpyF3I CTNAG 9 cut(s) 102, 441, 792, 918, 1167, 1269, 1707, 2024, 2583
HspAI GCGC 1 cut(s) 2474
KpnI GGTACC 1 cut(s) 747
KspI CCGCGG 1 cut(s) 2614
LmnI GCTCC 8 cut(s) 163, 374, 450, 605, 1300, 2116, 2327, 2592
Lsp1109I GCAGC 9 cut(s) 501, 550, 673, 680, 1222, 1462, 1831, 2470, 2498
LweI GCATC 9 cut(s) 80, 337, 601, 619, 713, 735, 1449, 1594, 2290
MaeI CTAG 7 cut(s) 84, 450, 689, 1434, 2007, 2337, 2592
MaeIII GTNAC 4 cut(s) 412, 499, 1186, 2554
MbiI CCGCTC 1 cut(s) 1072
MfeI CAATTG 3 cut(s) 585, 1262, 2451
MflI RGATCY 1 cut(s) 2518
MhlI GDGCHC 6 cut(s) 361, 447, 645, 1496, 2314, 2589
MlsI TGGCCA 1 cut(s) 1870
MluNI TGGCCA 1 cut(s) 1870
MlyI GAGTC 3 cut(s) 135, 710, 2088
MmeI TCCRAC 3 cut(s) 718, 1123, 1126
Mox20I TGGCCA 1 cut(s) 1870
Mph1103I ATGCAT 2 cut(s) 634, 728
MroXI GAANNNNTTC 2 cut(s) 1636, 1723
MscI TGGCCA 1 cut(s) 1870
MslI CAYNNNNRTG 2 cut(s) 2352, 2464
Msp20I TGGCCA 1 cut(s) 1870
MspA1I CMGCKG 1 cut(s) 2613
MspCI CTTAAG 3 cut(s) 65, 655, 1988
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 5 cut(s) 300, 846, 878, 1818, 2253
MunI CAATTG 3 cut(s) 585, 1262, 2451
Mva1269I GAATGC 2 cut(s) 187, 930
MvaI CCWGG 5 cut(s) 300, 846, 878, 1818, 2253
MvnI CGCG 1 cut(s) 2613
MwoI GCNNNNNNNGC 5 cut(s) 451, 1054, 1075, 2480, 2593
NlaIV GGNNCC 6 cut(s) 370, 475, 745, 1296, 2323, 2617
NmuCI GTSAC 4 cut(s) 412, 499, 1186, 2554
NsbI TGCGCA 1 cut(s) 2475
NsiI ATGCAT 2 cut(s) 634, 728
NspI RCATGY 3 cut(s) 355, 1242, 1333
PaeI GCATGC 2 cut(s) 1242, 1333
PaeR7I CTCGAG 1 cut(s) 1073
PagI TCATGA 2 cut(s) 709, 2599
PceI AGGCCT 2 cut(s) 1057, 1165
PciI ACATGT 1 cut(s) 351
PctI GAATGC 2 cut(s) 187, 930
PdmI GAANNNNTTC 2 cut(s) 1636, 1723
PfeI GAWTC 5 cut(s) 134, 214, 994, 1931, 2087
PflFI GACNNNGTC 2 cut(s) 410, 650
PinAI ACCGGT 1 cut(s) 746
PleI GAGTC 3 cut(s) 134, 710, 2087
PpsI GAGTC 3 cut(s) 134, 710, 2087
PscI ACATGT 1 cut(s) 351
Psp124BI GAGCTC 2 cut(s) 447, 2589
Psp6I CCWGG 5 cut(s) 298, 844, 876, 1816, 2251
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 5 cut(s) 298, 844, 876, 1816, 2251
PspN4I GGNNCC 6 cut(s) 370, 475, 745, 1296, 2323, 2617
PspPI GGNCC 4 cut(s) 466, 1652, 1811, 2608
PspXI VCTCGAGB 1 cut(s) 1073
PstI CTGCAG 2 cut(s) 2446, 2488
PstNI CAGNNNCTG 2 cut(s) 440, 2582
PsuI RGATCY 1 cut(s) 2518
PsyI GACNNNGTC 2 cut(s) 410, 650
RseI CAYNNNNRTG 2 cut(s) 2352, 2464
SacI GAGCTC 2 cut(s) 447, 2589
SacII CCGCGG 1 cut(s) 2614
Sau96I GGNCC 4 cut(s) 466, 1652, 1811, 2608
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 3 cut(s) 135, 710, 2088
ScrFI CCNGG 5 cut(s) 300, 846, 878, 1818, 2253
SduI GDGCHC 6 cut(s) 361, 447, 645, 1496, 2314, 2589
SfaNI GCATC 9 cut(s) 80, 337, 601, 619, 713, 735, 1449, 1594, 2290
SfcI CTRYAG 4 cut(s) 1140, 1862, 2442, 2484
Sfr274I CTCGAG 1 cut(s) 1073
Sfr303I CCGCGG 1 cut(s) 2614
SgrBI CCGCGG 1 cut(s) 2614
SinI GGWCC 3 cut(s) 466, 1652, 2608
SlaI CTCGAG 1 cut(s) 1073
SmiMI CAYNNNNRTG 2 cut(s) 2352, 2464
SmlI CTYRAG 7 cut(s) 65, 655, 985, 1073, 1289, 1444, 1988
SmoI CTYRAG 7 cut(s) 65, 655, 985, 1073, 1289, 1444, 1988
SpeI ACTAGT 1 cut(s) 1433
SphI GCATGC 2 cut(s) 1242, 1333
SseBI AGGCCT 2 cut(s) 1057, 1165
SsiI CCGC 6 cut(s) 895, 1070, 1242, 1847, 2611, 2613
SspI AATATT 2 cut(s) 322, 2275
SspMI CTAG 7 cut(s) 84, 450, 689, 1434, 2007, 2337, 2592
SstI GAGCTC 2 cut(s) 447, 2589
StuI AGGCCT 2 cut(s) 1057, 1165
StyD4I CCNGG 5 cut(s) 298, 844, 876, 1816, 2251
StyI CCWWGG 4 cut(s) 332, 449, 2285, 2591
TaqI TCGA 4 cut(s) 645, 1074, 1822, 1929
TatI WGTACW 9 cut(s) 15, 32, 755, 883, 1285, 1687, 1884, 1938, 2435
TauI GCSGC 1 cut(s) 1072
TfiI GAWTC 5 cut(s) 134, 214, 994, 1931, 2087
TseFI GTSAC 4 cut(s) 412, 499, 1186, 2554
TseI GCWGC 9 cut(s) 489, 563, 661, 668, 1235, 1450, 1844, 2483, 2486
Tsp45I GTSAC 4 cut(s) 412, 499, 1186, 2554
Tth111I GACNNNGTC 2 cut(s) 410, 650
Vha464I CTTAAG 3 cut(s) 65, 655, 1988
VpaK11BI GGWCC 3 cut(s) 466, 1652, 2608
XapI RAATTY 5 cut(s) 221, 968, 1723, 1994, 2174
XceI RCATGY 3 cut(s) 355, 1242, 1333
XhoI CTCGAG 1 cut(s) 1073
XmaJI CCTAGG 2 cut(s) 449, 2591
XmnI GAANNNNTTC 2 cut(s) 1636, 1723
XspI CTAG 7 cut(s) 84, 450, 689, 1434, 2007, 2337, 2592
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 2 cut(s) 634, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.