Rh2BG545100

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001253
Physical Location & Seq
Reverse (-)
75299772 .. 75308584
8813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG545100.1

Sequence Viewer

Length: 2469 bp
ATGGTATATTTGACACAAACTGTCATATTTTTCAAGATGGAGGATTTGCCTCCACCACCTCCTATTGTACCACCGAATGTGAAGCCAGAAGTTGTGGCTCTGGCTCCTCCTAAGCACTCTATTATGGTTAGGCCACGTGGGAGTGGAACCACAGGGCGGCGCATACAATTGAGCACTAACCACTTCAAAGTTTCTGTGAAGAATCCAGATGCTGTGTTCTACCAGTACTCTGTTCTGATAACTTATGAAGATAAGAGACCTGTTGAAGGCATGGGACTTGGGAGAAAGCTGGTGGATAAGCTCTATCAAACATACTCTTCAGAGTTTGCTGGTAAAAAGTTTGCATATGATGGGGAGAAAGCTTTATACACAGTCGGTCCTCTCCCGCGAAACAAGCTTGAGTTCACAGTTGTTTTGGAGGAGTCATTTACAAAGAGCAACAAAATTGGGAAGTCTGGTGAGACTGATAAAAGGATGAAGCACTGTTCCAATCGGTCGAAAACATTCAATGTAGAGATAAGTTATGCTGCCAAAATAACTTTCAAGTCAATTGCTCTTGCCCTTCAAGGAGCTGAAGTAGACAACACTCAGGATGCATTGAGAGTGCTTGATATTATTCTACGGCAGAAAGCAGCTAAGATGGGATGCCTTCTAGTACGACAGTCATTCTTCCATGATGACTCCAGGAACTTTGTTGAGATTGGAGGAGGTGTGACTGGTGTCCGGGGTTTTCATTCCAGTTTCCGTCCAACTCAGGGGGGCATGTCCTTGAATATGGATGTATCTACAACAATGATTGTGACGCCTGGACCTGTTATTGATTTTCTGTTAACCAACCAGGGTGTACGAGAACCCCGCTACATTGACTGGGTAAAGGCCAAAAGAATGCTGAAAAATATGAGGATTACAACGAGACACCGTAACATGGAGTGCAAAATCACTGGGTTGAGTGAGAAGACCTGCAATAATCAGCTGTTTAATCTGAAGCTAAGGAGTGGTGATGGCATGAGTGAGGGGGAAGCTGTGGAGATTACGGTGTATGAATATTTCGTCAAACACTGTGGCATAGAGCTCACCTATTCTCAACACATGCCATGCCTTGATGTTGGCAAGCCAAAGAGGCCCACTTATGTTCCAGTTGAGCTATGTTCACTTGTTTCACTACAAAGGTATACAAAAGCATTATCTTCCCAGCAAAGAGCCTCCTTAGTGGAAAAATCAAGGCAGAAGCCTCTGGAGAGAATAAAGAGTGTGACTGATGCTTTAAGAAATTACCGCTATGATGAGGATCCTGTGCTTGTTGAATGTGGTATATCTATAGAGAAGCAACTGATTCAGATGGATGGCCGTATTCTTGAGACCCCAAAGTTAAAGGTCGGTGGCAATGAGGACATGATGCCCTTTAAGGGACGATGGAACTTCAAAGGAAAGTCATTTTTGGATCCTACTCGCATTAACTCTTGGCTAGCTGTCAACTTCTCCGGACGCTGTGACCTTAGTCAAATCTCTCGGGAGCTTATCAATGTTGGGAAGAAGACTGGAATTCATATTGAGCGACCCAAAACTCTAATTGAGGAAGATCCGCGAGCTGAAAGACTAGGCCCTGTTGCTAGAGTAGACAAGATGTTTGAACAGATTCAGGCTAAGCTCCAAGAACCACCTGAGTTCATTCTGTGTGTCATACCCAAGAAAGGTTCTGATATCTATGGGCCTTGGAAGAAAAAAACTCTGGTTGACTTTGGGATTCCAACACAATGTATTTCCCCACCCAAGCTTAGCGATCCTTACCTGACTAATGTCCTAATGAAAATCAATACCAAGCTTGGAGGAATAAATTCTCTCTTGGCACTGGAGTACTCCTCATGTGTTCCTCTTATAAATGATACTCCTACAATGATTTTGGGGATGGATGTGTCTCATGGTTCTCCTGGCCGTTCAGATATTCCTTCAGTTGCTGCGGTTGTTGGCTCTCGTAGTTGGCCTCTTATATCAAGGTACAGAGCAGCTGTTAGAACACAATCTCCTAAGGTGGAGATGATTGATGGTCTATACAAGCCACTTCCAAATGGTAATGATGATGGTATTATCAGGGAACTGCTTCTGGACTTCTATAACACTAGCAAAGGACGCAAACCAACTCAGATAATTGTTTTCAGGTATTACAAGATTGTCTTGTTATCATGTTTTGGTCTATCAAAAGCGCCATCCTTCAAAGGGAAACCCGAGTACAGGCATAATAATCTTGCAGCTTACCAGAACCTTGGGGAGGAAAATGTTCCAAAGTTCACTCTCATTATAGCTCAAAAGAATCATCACACAAAGCTATTCCAAGCTAATAGCCCTGACAATGTTCCACCAGGGACAGTTGTTGACACAAAAATCGTGCACCCAAGGATCTATGATTTCTACATGTGTGCTCAGGCTGGCATGATTGTACCAAAGGAGCTGTACTGCAATATCCATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

822

Amino Acids

92.62

Weight (kDa)

9.44

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 53 - 207 5.6e-31 N-terminal domain of argonaute
ArgoL1 PF08699 219 - 267 8.6e-18 Argonaute linker 1 domain
PAZ PF02170 273 - 403 6e-29 PAZ domain
ArgoL2 PF16488 413 - 458 1.9e-11 Argonaute linker 2 domain
Piwi PF02171 556 - 811 3.2e-56 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1877
Acc36I ACCTGC 1 cut(s) 968
AccI GTMKAC 3 cut(s) 579, 1172, 1617
AccII CGCG 2 cut(s) 388, 1585
AccIII TCCGGA 1 cut(s) 1481
AciI CCGC 6 cut(s) 157, 386, 856, 1276, 1583, 1958
AclWI GGATC 7 cut(s) 1283, 1296, 1436, 1449, 1574, 1775, 2402
AcoI YGGCCR 2 cut(s) 1345, 1930
AcsI RAATTY 2 cut(s) 1542, 1834
AcuI CTGAAG 4 cut(s) 303, 594, 1004, 1932
AcvI CACGTG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 9 cut(s) 69, 227, 657, 846, 1856, 1997, 2228, 2436, 2450
AfiI CCNNNNNNNGG 9 cut(s) 156, 266, 755, 925, 1406, 1691, 2214, 2229, 2266
AflIII ACRYGT 1 cut(s) 2409
AjnI CCWGG 5 cut(s) 683, 805, 837, 1927, 2356
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AloI GAACNNNNNNTCC 2 cut(s) 2005, 2037
Alw21I GWGCWC 4 cut(s) 176, 1074, 2388, 2419
Alw26I GTCTC 5 cut(s) 250, 455, 907, 1352, 1920
Alw44I GTGCAC 1 cut(s) 2384
AlwI GGATC 7 cut(s) 1283, 1296, 1436, 1449, 1574, 1775, 2402
AlwNI CAGNNNCTG 2 cut(s) 212, 1955
Ama87I CYCGRG 2 cut(s) 1509, 2222
Aor13HI TCCGGA 1 cut(s) 1481
AoxI GGCC 8 cut(s) 131, 876, 1121, 1345, 1600, 1709, 1930, 1979
ApaLI GTGCAC 1 cut(s) 2384
ApeKI GCWGC 5 cut(s) 527, 632, 1955, 2003, 2246
ApoI RAATTY 2 cut(s) 1542, 1834
ArsI GACNNNNNNTTYG 2 cut(s) 1610, 1642
Asp700I GAANNNNTTC 5 cut(s) 503, 1635, 1834, 2097, 2274
AspLEI GCGC 2 cut(s) 162, 2203
AspS9I GGNCC 5 cut(s) 377, 809, 1122, 1601, 1709
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 3 cut(s) 470, 1010, 1066
AsuNHI GCTAGC 1 cut(s) 1465
AvaI CYCGRG 2 cut(s) 1509, 2222
AvaII GGWCC 2 cut(s) 377, 809
AxyI CCTNAGG 1 cut(s) 2025
BaeGI GKGCMC 1 cut(s) 2388
BamHI GGATCC 2 cut(s) 1288, 1441
BanII GRGCYC 1 cut(s) 1074
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 962, 1541
Bbv12I GWGCWC 4 cut(s) 176, 1074, 2388, 2419
BbvI GCAGC 5 cut(s) 514, 644, 1942, 2015, 2258
BceAI ACGGC 3 cut(s) 638, 1332, 1917
BciT130I CCWGG 5 cut(s) 685, 807, 839, 1929, 2358
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 5 cut(s) 250, 455, 907, 1352, 1920
BfaI CTAG 5 cut(s) 653, 1466, 1598, 1611, 2118
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 1 cut(s) 2204
BfuAI ACCTGC 1 cut(s) 968
BglI GCCNNNNNGGC 1 cut(s) 1120
BisI GCNGC 6 cut(s) 158, 528, 633, 1956, 2004, 2247
BlpI GCTNAGC 2 cut(s) 1644, 1775
BlsI GCNGC 6 cut(s) 159, 529, 634, 1957, 2005, 2248
BmcAI AGTACT 2 cut(s) 227, 1856
Bme1390I CCNGG 6 cut(s) 685, 725, 807, 839, 1929, 2358
Bme18I GGWCC 2 cut(s) 377, 809
BmeT110I CYCGRG 2 cut(s) 1509, 2222
BmgT120I GGNCC 5 cut(s) 377, 809, 1122, 1601, 1709
BmiI GGNNCC 4 cut(s) 105, 148, 1290, 1443
BmrFI CCNGG 6 cut(s) 685, 725, 807, 839, 1929, 2358
BmrI ACTGGG 2 cut(s) 877, 951
BmsI GCATC 5 cut(s) 199, 583, 635, 1249, 1386
BmtI GCTAGC 1 cut(s) 1469
BmuI ACTGGG 2 cut(s) 877, 951
BoxI GACNNNNGTC 3 cut(s) 719, 1497, 1796
BpiI GAAGAC 2 cut(s) 962, 1541
BplI GAGNNNNNCTC 2 cut(s) 1844, 1876
BpmI CTGGAG 3 cut(s) 667, 1256, 1871
Bpu10I CCTNAGC 3 cut(s) 111, 989, 2418
Bpu1102I GCTNAGC 2 cut(s) 1644, 1775
BpuEI CTTGAG 2 cut(s) 419, 1376
BpuMI CCSGG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 1287
BsaHI GRCGYC 1 cut(s) 803
BsaI GGTCTC 2 cut(s) 250, 1352
BsaJI CCNNGG 6 cut(s) 724, 838, 1712, 2260, 2357, 2390
BsaWI WCCGGW 1 cut(s) 1481
BsaXI ACNNNNNCTCC 6 cut(s) 561, 591, 696, 726, 2005, 2035
Bsc4I CCNNNNNNNGG 9 cut(s) 156, 266, 755, 925, 1406, 1691, 2214, 2229, 2266
Bse1I ACTGG 8 cut(s) 223, 721, 738, 872, 946, 1136, 1543, 1854
Bse21I CCTNAGG 1 cut(s) 2025
Bse3DI GCAATG 1 cut(s) 1390
Bse8I GATNNNNATC 1 cut(s) 1287
BseAI TCCGGA 1 cut(s) 1481
BseBI CCWGG 5 cut(s) 685, 807, 839, 1929, 2358
BseDI CCNNGG 6 cut(s) 724, 838, 1712, 2260, 2357, 2390
BseGI GGATG 8 cut(s) 480, 598, 650, 784, 1348, 1911, 1915, 2204
BseJI GATNNNNATC 1 cut(s) 1287
BseLI CCNNNNNNNGG 9 cut(s) 156, 266, 755, 925, 1406, 1691, 2214, 2229, 2266
BseMI GCAATG 1 cut(s) 1390
BseMII CTCAG 5 cut(s) 602, 767, 1653, 2153, 2432
BseNI ACTGG 8 cut(s) 223, 721, 738, 872, 946, 1136, 1543, 1854
BseRI GAGGAG 4 cut(s) 96, 434, 720, 1849
BseSI GKGCMC 1 cut(s) 2388
BseXI GCAGC 5 cut(s) 514, 644, 1942, 2015, 2258
BseYI CCCAGC 1 cut(s) 1191
Bsh1236I CGCG 2 cut(s) 388, 1585
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 8 cut(s) 133, 878, 1123, 1347, 1602, 1711, 1932, 1981
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 4 cut(s) 176, 1074, 2388, 2419
BsiHKCI CYCGRG 2 cut(s) 1509, 2222
BsiSI CCGG 2 cut(s) 724, 1482
BslFI GGGAC 3 cut(s) 288, 1422, 2374
BslI CCNNNNNNNGG 9 cut(s) 156, 266, 755, 925, 1406, 1691, 2214, 2229, 2266
BsmAI GTCTC 5 cut(s) 250, 455, 907, 1352, 1920
BsmFI GGGAC 3 cut(s) 288, 1422, 2374
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 8 cut(s) 133, 878, 1123, 1347, 1602, 1711, 1932, 1981
Bso31I GGTCTC 2 cut(s) 250, 1352
BsoBI CYCGRG 2 cut(s) 1509, 2222
Bsp1286I GDGCHC 4 cut(s) 176, 1074, 2388, 2419
Bsp13I TCCGGA 1 cut(s) 1481
Bsp143I GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
Bsp1720I GCTNAGC 2 cut(s) 1644, 1775
BspACI CCGC 6 cut(s) 157, 386, 856, 1276, 1583, 1958
BspANI GGCC 8 cut(s) 133, 878, 1123, 1347, 1602, 1711, 1932, 1981
BspCNI CTCAG 5 cut(s) 601, 766, 1654, 2152, 2431
BspEI TCCGGA 1 cut(s) 1481
BspFNI CGCG 2 cut(s) 388, 1585
BspLI GGNNCC 4 cut(s) 105, 148, 1290, 1443
BspMI ACCTGC 1 cut(s) 968
BspOI GCTAGC 1 cut(s) 1469
BspPI GGATC 7 cut(s) 1283, 1296, 1436, 1449, 1574, 1775, 2402
BspTNI GGTCTC 2 cut(s) 250, 1352
BsrDI GCAATG 1 cut(s) 1390
BsrI ACTGG 8 cut(s) 223, 721, 738, 872, 946, 1136, 1543, 1854
BssECI CCNNGG 6 cut(s) 724, 838, 1712, 2260, 2357, 2390
BssMI GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
BssNAI GTATAC 1 cut(s) 1173
BssNI GRCGYC 1 cut(s) 803
BssT1I CCWWGG 3 cut(s) 1712, 2260, 2390
Bst1107I GTATAC 1 cut(s) 1173
Bst2UI CCWGG 5 cut(s) 685, 807, 839, 1929, 2358
Bst4CI ACNGT 9 cut(s) 22, 373, 409, 485, 663, 920, 1036, 1061, 2365
Bst6I CTCTTC 1 cut(s) 322
BstACI GRCGYC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 4 cut(s) 1112, 1467, 1587, 2425
BstENI CCTNNNNNAGG 2 cut(s) 264, 2264
BstF5I GGATG 8 cut(s) 480, 598, 650, 784, 1348, 1911, 1915, 2204
BstFNI CGCG 2 cut(s) 388, 1585
BstH2I RGCGCY 1 cut(s) 2204
BstHHI GCGC 2 cut(s) 162, 2203
BstKTI GATC 5 cut(s) 1291, 1444, 1582, 1783, 2397
BstMAI GTCTC 5 cut(s) 250, 455, 907, 1352, 1920
BstMBI GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 3 cut(s) 394, 1120, 2127
BstNI CCWGG 5 cut(s) 685, 807, 839, 1929, 2358
BstNSI RCATGY 3 cut(s) 766, 1093, 2413
BstPAI GACNNNNGTC 3 cut(s) 719, 1497, 1796
BstSCI CCNGG 6 cut(s) 683, 723, 805, 837, 1927, 2356
BstSFI CTRYAG 1 cut(s) 1317
BstSLI GKGCMC 1 cut(s) 2388
BstUI CGCG 2 cut(s) 388, 1585
BstV1I GCAGC 5 cut(s) 514, 644, 1942, 2015, 2258
BstV2I GAAGAC 2 cut(s) 962, 1541
BstX2I RGATCY 4 cut(s) 1288, 1441, 1579, 2394
BstXI CCANNNNNNTGG 1 cut(s) 2261
BstYI RGATCY 4 cut(s) 1288, 1441, 1579, 2394
BstZ17I GTATAC 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 2025
BsuRI GGCC 8 cut(s) 133, 878, 1123, 1347, 1602, 1711, 1932, 1981
BtsCI GGATG 8 cut(s) 480, 598, 650, 784, 1348, 1911, 1915, 2204
BtsIMutI CAGTG 4 cut(s) 481, 939, 1057, 1847
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 4 cut(s) 1112, 1467, 1587, 2425
CaiI CAGNNNCTG 2 cut(s) 212, 1955
CfoI GCGC 2 cut(s) 162, 2203
Cfr13I GGNCC 5 cut(s) 377, 809, 1122, 1601, 1709
CseI GACGC 3 cut(s) 811, 1494, 2136
Csp6I GTAC 9 cut(s) 68, 226, 656, 845, 1855, 1996, 2227, 2435, 2449
CspCI CAANNNNNGTGG 2 cut(s) 1042, 1077
CviQI GTAC 9 cut(s) 68, 226, 656, 845, 1855, 1996, 2227, 2435, 2449
DpnI GATC 5 cut(s) 1290, 1443, 1581, 1782, 2396
DpnII GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
EaeI YGGCCR 2 cut(s) 1345, 1930
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 3 cut(s) 1712, 2260, 2390
Eco24I GRGCYC 1 cut(s) 1074
Eco31I GGTCTC 2 cut(s) 250, 1352
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 2 cut(s) 377, 809
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 4 cut(s) 303, 594, 1004, 1932
Eco72I CACGTG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 2025
Eco88I CYCGRG 2 cut(s) 1509, 2222
EcoICRI GAGCTC 1 cut(s) 1072
EcoNI CCTNNNNNAGG 2 cut(s) 264, 2264
EcoO109I RGGNCCY 1 cut(s) 1601
EcoRI GAATTC 1 cut(s) 1542
EcoRII CCWGG 5 cut(s) 683, 805, 837, 1927, 2356
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 3 cut(s) 1712, 2260, 2390
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 3 cut(s) 1712, 2260, 2390
FalI AAGNNNNNCTT 2 cut(s) 2156, 2188
FaqI GGGAC 3 cut(s) 288, 1422, 2374
FauI CCCGC 2 cut(s) 393, 863
FauNDI CATATG 1 cut(s) 346
FblI GTMKAC 3 cut(s) 579, 1172, 1617
Fnu4HI GCNGC 6 cut(s) 158, 528, 633, 1956, 2004, 2247
FokI GGATG 8 cut(s) 487, 605, 657, 791, 1355, 1918, 1922, 2191
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 6 cut(s) 158, 528, 633, 1956, 2004, 2247
FspBI CTAG 5 cut(s) 653, 1466, 1598, 1611, 2118
GlaI GCGC 2 cut(s) 161, 2202
GluI GCNGC 6 cut(s) 158, 528, 633, 1956, 2004, 2247
GsaI CCCAGC 1 cut(s) 1195
GsuI CTGGAG 3 cut(s) 667, 1256, 1871
HaeII RGCGCY 1 cut(s) 2204
HaeIII GGCC 8 cut(s) 133, 878, 1123, 1347, 1602, 1711, 1932, 1981
HapII CCGG 2 cut(s) 724, 1482
HgaI GACGC 3 cut(s) 811, 1494, 2136
HhaI GCGC 2 cut(s) 162, 2203
Hin1I GRCGYC 1 cut(s) 803
Hin6I GCGC 2 cut(s) 160, 2201
HinP1I GCGC 2 cut(s) 160, 2201
HincII GTYRAC 4 cut(s) 831, 1474, 1735, 2371
HindII GTYRAC 4 cut(s) 831, 1474, 1735, 2371
HindIII AAGCTT 4 cut(s) 360, 395, 1772, 1820
HinfI GANTC 7 cut(s) 202, 422, 680, 1333, 1636, 1744, 2308
HpaI GTTAAC 1 cut(s) 831
HpaII CCGG 2 cut(s) 724, 1482
HphI GGTGA 3 cut(s) 470, 1010, 1066
Hpy188I TCNGA 7 cut(s) 237, 322, 984, 1338, 1699, 1939, 2142
Hpy188III TCNNGA 8 cut(s) 34, 206, 590, 1235, 1355, 1482, 1511, 2102
HpyAV CCTTC 5 cut(s) 260, 572, 659, 1956, 2218
HpyCH4III ACNGT 9 cut(s) 22, 373, 409, 485, 663, 920, 1036, 1061, 2365
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 7 cut(s) 344, 596, 933, 963, 2246, 2386, 2454
HpyF10VI GCNNNNNNNGC 3 cut(s) 394, 1120, 2127
HpySE526I ACGT 1 cut(s) 136
Hsp92I GRCGYC 1 cut(s) 803
HspAI GCGC 2 cut(s) 160, 2201
Kpn2I TCCGGA 1 cut(s) 1481
KspAI GTTAAC 1 cut(s) 831
Kzo9I GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
LmnI GCTCC 5 cut(s) 109, 569, 1513, 1653, 2443
Lsp1109I GCAGC 5 cut(s) 514, 644, 1942, 2015, 2258
LweI GCATC 5 cut(s) 199, 583, 635, 1249, 1386
MaeI CTAG 5 cut(s) 653, 1466, 1598, 1611, 2118
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 5 cut(s) 712, 799, 920, 1252, 1490
MalI GATC 5 cut(s) 1290, 1443, 1581, 1782, 2396
MboI GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
MfeI CAATTG 2 cut(s) 167, 549
MflI RGATCY 4 cut(s) 1288, 1441, 1579, 2394
MhlI GDGCHC 4 cut(s) 176, 1074, 2388, 2419
MluCI AATT 8 cut(s) 167, 444, 549, 1270, 1542, 1569, 1834, 2145
MlyI GAGTC 2 cut(s) 431, 674
MmeI TCCRAC 2 cut(s) 773, 1772
Mph1103I ATGCAT 1 cut(s) 598
MroI TCCGGA 1 cut(s) 1481
MroXI GAANNNNTTC 5 cut(s) 503, 1635, 1834, 2097, 2274
MseI TTAA 6 cut(s) 830, 978, 1265, 1370, 1404, 1455
MspA1I CMGCKG 2 cut(s) 973, 2006
MspI CCGG 2 cut(s) 724, 1482
MspR9I CCNGG 6 cut(s) 685, 725, 807, 839, 1929, 2358
MunI CAATTG 2 cut(s) 167, 549
Mva1269I GAATGC 1 cut(s) 891
MvaI CCWGG 5 cut(s) 685, 807, 839, 1929, 2358
MvnI CGCG 2 cut(s) 388, 1585
MwoI GCNNNNNNNGC 3 cut(s) 394, 1120, 2127
NciI CCSGG 1 cut(s) 725
NdeI CATATG 1 cut(s) 346
NdeII GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
NheI GCTAGC 1 cut(s) 1465
NlaIV GGNNCC 4 cut(s) 105, 148, 1290, 1443
NmuCI GTSAC 4 cut(s) 712, 799, 1252, 1490
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 3 cut(s) 766, 1093, 2413
PciI ACATGT 1 cut(s) 2409
PctI GAATGC 1 cut(s) 891
PdmI GAANNNNTTC 5 cut(s) 503, 1635, 1834, 2097, 2274
PfeI GAWTC 5 cut(s) 202, 1333, 1636, 1744, 2308
PfoI TCCNGGA 1 cut(s) 683
PkrI GCNGC 6 cut(s) 159, 529, 634, 1957, 2005, 2248
PleI GAGTC 2 cut(s) 430, 674
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 2 cut(s) 430, 674
Ppu21I YACGTR 1 cut(s) 137
PscI ACATGT 1 cut(s) 2409
PshAI GACNNNNGTC 3 cut(s) 719, 1497, 1796
PsiI TTATAA 1 cut(s) 1877
Psp124BI GAGCTC 1 cut(s) 1074
Psp6I CCWGG 5 cut(s) 683, 805, 837, 1927, 2356
PspCI CACGTG 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 1191
PspGI CCWGG 5 cut(s) 683, 805, 837, 1927, 2356
PspN4I GGNNCC 4 cut(s) 105, 148, 1290, 1443
PspPI GGNCC 5 cut(s) 377, 809, 1122, 1601, 1709
PstNI CAGNNNCTG 2 cut(s) 212, 1955
PsuI RGATCY 4 cut(s) 1288, 1441, 1579, 2394
PvuII CAGCTG 2 cut(s) 973, 2006
RsaI GTAC 9 cut(s) 69, 227, 657, 846, 1856, 1997, 2228, 2436, 2450
RsaNI GTAC 9 cut(s) 68, 226, 656, 845, 1855, 1996, 2227, 2435, 2449
SacI GAGCTC 1 cut(s) 1074
SaqAI TTAA 6 cut(s) 830, 978, 1265, 1370, 1404, 1455
SatI GCNGC 6 cut(s) 158, 528, 633, 1956, 2004, 2247
Sau3AI GATC 5 cut(s) 1288, 1441, 1579, 1780, 2394
Sau96I GGNCC 5 cut(s) 377, 809, 1122, 1601, 1709
ScaI AGTACT 2 cut(s) 227, 1856
SchI GAGTC 2 cut(s) 431, 674
ScrFI CCNGG 6 cut(s) 685, 725, 807, 839, 1929, 2358
SduI GDGCHC 4 cut(s) 176, 1074, 2388, 2419
SfaNI GCATC 5 cut(s) 199, 583, 635, 1249, 1386
SfcI CTRYAG 1 cut(s) 1317
SinI GGWCC 2 cut(s) 377, 809
SmlI CTYRAG 2 cut(s) 398, 1355
SmoI CTYRAG 2 cut(s) 398, 1355
Sse9I AATT 8 cut(s) 167, 444, 549, 1270, 1542, 1569, 1834, 2145
SsiI CCGC 6 cut(s) 157, 386, 856, 1276, 1583, 1958
SspI AATATT 1 cut(s) 1046
SspMI CTAG 5 cut(s) 653, 1466, 1598, 1611, 2118
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 6 cut(s) 683, 723, 805, 837, 1927, 2356
StyI CCWWGG 3 cut(s) 1712, 2260, 2390
TaaI ACNGT 9 cut(s) 22, 373, 409, 485, 663, 920, 1036, 1061, 2365
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 497
TaqII GACCGA 2 cut(s) 365, 483
TasI AATT 8 cut(s) 167, 444, 549, 1270, 1542, 1569, 1834, 2145
TatI WGTACW 4 cut(s) 225, 1854, 2226, 2448
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 5 cut(s) 202, 1333, 1636, 1744, 2308
Tru1I TTAA 6 cut(s) 830, 978, 1265, 1370, 1404, 1455
Tru9I TTAA 6 cut(s) 830, 978, 1265, 1370, 1404, 1455
TscAI CASTG 4 cut(s) 488, 946, 1064, 1854
TseFI GTSAC 4 cut(s) 712, 799, 1252, 1490
TseI GCWGC 5 cut(s) 527, 632, 1955, 2003, 2246
Tsp45I GTSAC 4 cut(s) 712, 799, 1252, 1490
TspDTI ATGAA 7 cut(s) 261, 491, 722, 1056, 1535, 1657, 1820
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 4 cut(s) 488, 946, 1064, 1854
VneI GTGCAC 1 cut(s) 2384
VpaK11BI GGWCC 2 cut(s) 377, 809
XagI CCTNNNNNAGG 2 cut(s) 264, 2264
XapI RAATTY 2 cut(s) 1542, 1834
XceI RCATGY 3 cut(s) 766, 1093, 2413
XmiI GTMKAC 3 cut(s) 579, 1172, 1617
XmnI GAANNNNTTC 5 cut(s) 503, 1635, 1834, 2097, 2274
XspI CTAG 5 cut(s) 653, 1466, 1598, 1611, 2118
ZrmI AGTACT 2 cut(s) 227, 1856
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.