RchiOBHm_Chr2g0157951

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001253
Physical Location & Seq
Reverse (-)
74272738 .. 74278333
5596 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52666

Sequence Viewer

Length: 2766 bp
ATGATGGTAGCTGGTGCCAAAAATGCAGGTGGGGACGCAGATTTGCCCCCACCCCCTCCAACCATACCATCCAATTTGAAGCCAGAACTATTGGCTCCGCCCAAGTATTCTATTATGACTAGGCCATGCAGGACTGGAACTACTGGACGGGGCATAACTTTGCTTGCCAACCACTTCAAAGTTTCAGTGAATAATCCAGATGCTGTATTTTATCAGTATTCTGTTCACATGACTTATGAAGATAATAATCCAGTTCTTCCAGGGATGGGAATTGGGAGAAAATTGATGGATAGGCTTTATCATACATACTCCATGGAATTTGATGGCAACAAATTTGCATACGATGGGGAGAAATCTCTGTACACATTTGGTCCTCTACCACTGCACAAGCTCAATTACACAGTTGTGCTGGAGGAATCAGTTTCTAGGCCACATGTCACTGAAAAACGGATGAAACTATCTATTCAGTCAAAGACTTTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAGCCAATTGCTCTTGCCCTTAAAGGAGCTGATGCAGATGATGGTCAAGATGCATTGAGAGTGCTAGACATAGTCTTAAGGCAGCAAGCAGCTAACAGGGGATGTCTTCTAGTAAGACAGTCATTCTTTCAGGATGACTCCAGATGCATCACTGCTGTTGGAGGTACCGGTTTAAGTACTGTTCGGGGCTTCCATTCCAGTTTTCGTCCCACTCAGGGTGGTCTGACCTTGAATATGGGTACAAAATTTTGTTGCCTTTTTGTAATCTCTGTGGTTGCCTACACTTGTCGTCACTTGTCACTTTTTCTGTTATGGCTTCTTGCAGATGTATCTACTACACTGATCTTAACCCCTGGACCAGTGATTGATTTTCTGAAAGCTAACCAGGGTGTACAACACCCCCGCAACATTGACTGGGCAAGGGCTAAAAGAATGCTGAAAAATTTGAGGATAAAGACAAGACATCACAACATGGAATTCAAAATTACTGGCTTGAGCCAGAATCCCTGCCGTGAGCAATATGATGACGGAACTACTGTGGAGCAGACTGTGGACATTACTGTATATGAGTATTTCACCAAACATCGTGGCATAGAACTTACCTATTCTGCAAACTTTCCATGCCTTGACGTTGGCAAGCCAAAAAGGCCTAACTATGTGCCACTCGAGCTTTGTTCCCTTGTTTCCCTCCAACGGTATAAAAAAGCATTATCTTCAAAGCAGAGAGCTTCTATAGTTGGAAGTTCAAGGCAGAGGCCTCAGGAGAGAATAAATAGTGTGACTAATGCTGTAAGGAATTATGGCTATAATGATGATCCTGTGCTTGCTGCATGCGGTATATCCATAGAGAAGCAATTGACTCAGATTGAAGGTCGTGTACTTGAGGCTCCGAAGTTGAAGGTAGGCAACAGTTCCGATTGCATGCCTTCAGATGGGAGATGGAACTTCAATGGCAAGACATTTTTGAACCCCAAACACATTGACAAATGGATTGTTGTCAACTTCTCTTTGGAAAATTGTGATACTAGTCGCATCTCAAGGCAGCTTTATGACATTGGGAGGAAGAAGGGCATTAGAATTGAGTACCCAATCACATTGATTGAGGAACAACCACAGCTTAAAAAGCAAAGCCCTATTGAAAGAGTTAAAAAGATGTTTGAACAGATTAAAGTGAAGCATCCTACAGAGCCACCAAACTTCATTCTTTGCGTTTTACCAGAAAGAAAAACTTCTGATATATATGGACCGTGGAAGAAAGAGTGCCTTGTTGACTATGGCATTGTCACACAGTGCATTTCTCCTCCCATGAAAAAAAATAATAGGGAGAGAGAGCTATCTGAACAGTACCTCACTAATCTTGGAGGAATAAATTCTTTGTTGGCAACAGAACACTCTGGGGTTGTTTCTCTAATTAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGCCTCCTGGTCGATCAGATATTCCTTCTGTTGCTGCGGTTGTTGGCTCTCTACAGTGGCCACTAATTTCACAGTACAGAGCGGCTGTAAGGACACAAAAAGTTCGTAAGGAGATGATTGATTCTCTGTACAAGCCACTGGAAAGTGGAGAAGATGATGGTATTATGAGGGAACTGCTTAAGGAATTCTATAAAACTAGCAAATGCAAACCAACTCAGATTATTATCTTCAGGGATGGAGTCAGTGAATCGCAATTTAATCAAGTTTTGAATGAGGAGCTGGATCAAATTATGAAGGCTTACAAGCACCACCAGAAGGTTGATGAGCTTCCAAAATTTACTGTGATTGTGGCTCAAAAGAACCATAATACAAAGCTATTCCAAGCTGGTTCTCCCCAGTATAATGTTCCACCAGGAACAGTTGTGGACACAAATATTGTGCATCCAAGGAATTATGATTTCTATATGTGTGCTCACAAAGGCTCCATTGGAACTTCTAGGCCAGTACACTATCATGTCTTGCTCGATGAGATTGGCTTTTCTGCCAATGAACTGCAAAACCTGATCCACTCTCTTTCCTATGTGTACCAGAGGAGTACAACTGCAGTCTCAATTGTTGCGCCCATATGTTATGCGCACCTTGCTGCAGCCCAAGTAATGCAGTTTATGAAGTTTGAAGATCTCTCGGCAACTTCATCATCAGGAAGGGTCAGTGTCACTACATCAGAGAGTATCCCAGTGCCTGAGCTCCCTAGGCTTCATGAGAAGGTCCGCAGTTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

921

Amino Acids

103.52

Weight (kDa)

9.14

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 51 - 198 9.8e-25 N-terminal domain of argonaute
ArgoL1 PF08699 210 - 251 4.9e-10 Argonaute linker 1 domain
PAZ PF02170 294 - 417 1.2e-28 PAZ domain
ArgoL2 PF16488 427 - 472 1.5e-14 Argonaute linker 2 domain
ArgoMid PF16487 480 - 561 1.6e-06 Mid domain of argonaute
Piwi PF02171 572 - 881 4.1e-95 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 2607
Acc36I ACCTGC 1 cut(s) 17
Acc65I GGTACC 1 cut(s) 680
AccB1I GGYRCC 2 cut(s) 14, 680
AccBSI CCGCTC 1 cut(s) 2054
AciI CCGC 6 cut(s) 98, 919, 1350, 2009, 2054, 2743
AclWI GGATC 3 cut(s) 1325, 2262, 2530
AcoI YGGCCR 1 cut(s) 2030
AcsI RAATTY 8 cut(s) 317, 332, 761, 958, 992, 1885, 2156, 2306
AcuI CTGAAG 3 cut(s) 536, 1428, 2185
AdeI CACNNNGTG 1 cut(s) 1806
AfiI CCNNNNNNNGG 5 cut(s) 266, 731, 1209, 1448, 2287
AflII CTTAAG 2 cut(s) 592, 2150
AflIII ACRYGT 1 cut(s) 433
AgeI ACCGGT 1 cut(s) 683
AhlI ACTAGT 1 cut(s) 1541
AjnI CCWGG 5 cut(s) 259, 868, 900, 1978, 2383
AleI CACNNNNGTG 1 cut(s) 404
Alw21I GWGCWC 2 cut(s) 2446, 2721
Alw26I GTCTC 1 cut(s) 2584
AlwI GGATC 3 cut(s) 1325, 2262, 2530
AlwNI CAGNNNCTG 2 cut(s) 203, 2714
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 7 cut(s) 122, 428, 1163, 1271, 1973, 2030, 2471
ApeKI GCWGC 8 cut(s) 500, 598, 605, 1343, 1558, 2006, 2615, 2618
ApoI RAATTY 8 cut(s) 317, 332, 761, 958, 992, 1885, 2156, 2306
AsiGI ACCGGT 1 cut(s) 683
Asp700I GAANNNNTTC 2 cut(s) 1744, 1885
Asp718I GGTACC 1 cut(s) 680
AspA2I CCTAGG 1 cut(s) 2723
AspLEI GCGC 2 cut(s) 2593, 2608
AspS9I GGNCC 5 cut(s) 371, 872, 1760, 1973, 2740
AsuHPI GGTGA 1 cut(s) 1084
AvaI CYCGRG 1 cut(s) 1181
AvaII GGWCC 4 cut(s) 371, 872, 1760, 2740
AvrII CCTAGG 1 cut(s) 2723
AxyI CCTNAGG 1 cut(s) 1275
BalI TGGCCA 1 cut(s) 2032
BanI GGYRCC 2 cut(s) 14, 680
BanII GRGCYC 1 cut(s) 2721
BarI GAAGNNNNNNTAC 2 cut(s) 1377, 1409
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 2 cut(s) 2446, 2721
BbvI GCAGC 8 cut(s) 487, 610, 617, 1330, 1570, 1993, 2602, 2630
BceAI ACGGC 1 cut(s) 1011
BciT130I CCWGG 5 cut(s) 261, 870, 902, 1980, 2385
BciVI GTATCC 1 cut(s) 2714
BcoDI GTCTC 1 cut(s) 2584
BcuI ACTAGT 1 cut(s) 1541
BfaI CTAG 8 cut(s) 120, 426, 581, 626, 1542, 2169, 2469, 2724
BfmI CTRYAG 5 cut(s) 1248, 1698, 2024, 2574, 2616
BfrI CTTAAG 2 cut(s) 592, 2150
BfuAI ACCTGC 1 cut(s) 17
BfuI GTATCC 1 cut(s) 2714
BglI GCCNNNNNGGC 1 cut(s) 1162
BglII AGATCT 1 cut(s) 2650
BisI GCNGC 9 cut(s) 501, 599, 606, 1344, 1559, 2007, 2055, 2616, 2619
BlnI CCTAGG 1 cut(s) 2723
BlsI GCNGC 9 cut(s) 502, 600, 607, 1345, 1560, 2008, 2056, 2617, 2620
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 5 cut(s) 261, 870, 902, 1980, 2385
Bme18I GGWCC 4 cut(s) 371, 872, 1760, 2740
BmeT110I CYCGRG 1 cut(s) 1181
BmgT120I GGNCC 5 cut(s) 371, 872, 1760, 1973, 2740
BmiI GGNNCC 5 cut(s) 16, 96, 682, 1404, 2455
BmrFI CCNGG 5 cut(s) 261, 870, 902, 1980, 2385
BmrI ACTGGG 3 cut(s) 940, 2362, 2702
BmsI GCATC 8 cut(s) 190, 538, 556, 650, 672, 1557, 1702, 2422
BmuI ACTGGG 3 cut(s) 940, 2362, 2702
BpiI GAAGAC 1 cut(s) 614
BpmI CTGGAG 2 cut(s) 431, 640
Bpu10I CCTNAGC 1 cut(s) 2715
BpuEI CTTGAG 3 cut(s) 1030, 1418, 1537
BsaBI GATNNNNATC 2 cut(s) 246, 2195
BsaJI CCNNGG 7 cut(s) 260, 312, 868, 901, 1763, 2417, 2723
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 2438, 2468
Bsc4I CCNNNNNNNGG 5 cut(s) 266, 731, 1209, 1448, 2287
Bse118I RCCGGY 1 cut(s) 683
Bse21I CCTNAGG 1 cut(s) 1275
Bse8I GATNNNNATC 2 cut(s) 246, 2195
BseBI CCWGG 5 cut(s) 261, 870, 902, 1980, 2385
BseDI CCNNGG 7 cut(s) 260, 312, 868, 901, 1763, 2417, 2723
BseJI GATNNNNATC 2 cut(s) 246, 2195
BseLI CCNNNNNNNGG 5 cut(s) 266, 731, 1209, 1448, 2287
BseMII CTCAG 5 cut(s) 743, 1289, 1391, 2201, 2706
BseRI GAGGAG 3 cut(s) 1806, 2261, 2578
BseXI GCAGC 8 cut(s) 487, 610, 617, 1330, 1570, 1993, 2602, 2630
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 7 cut(s) 124, 430, 1165, 1273, 1975, 2032, 2473
BshNI GGYRCC 2 cut(s) 14, 680
BshTI ACCGGT 1 cut(s) 683
BsiHKAI GWGCWC 2 cut(s) 2446, 2721
BsiHKCI CYCGRG 1 cut(s) 1181
BsiSI CCGG 1 cut(s) 684
BslFI GGGAC 2 cut(s) 47, 708
BslI CCNNNNNNNGG 5 cut(s) 266, 731, 1209, 1448, 2287
BsmAI GTCTC 1 cut(s) 2584
BsmFI GGGAC 2 cut(s) 47, 708
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 7 cut(s) 124, 430, 1165, 1273, 1975, 2032, 2473
BsoBI CYCGRG 1 cut(s) 1181
Bsp1286I GDGCHC 2 cut(s) 2446, 2721
Bsp1407I TGTACA 3 cut(s) 360, 907, 2100
Bsp143I GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 6 cut(s) 98, 919, 1350, 2009, 2054, 2743
BspANI GGCC 7 cut(s) 124, 430, 1165, 1273, 1975, 2032, 2473
BspCNI CTCAG 5 cut(s) 742, 1288, 1390, 2200, 2707
BspHI TCATGA 1 cut(s) 2731
BspLI GGNNCC 5 cut(s) 16, 96, 682, 1404, 2455
BspMAI CTGCAG 2 cut(s) 2578, 2620
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 1325, 2262, 2530
BspT107I GGYRCC 2 cut(s) 14, 680
BspTI CTTAAG 2 cut(s) 592, 2150
BsrBI CCGCTC 1 cut(s) 2054
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 3 cut(s) 360, 907, 2100
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 7 cut(s) 260, 312, 868, 901, 1763, 2417, 2723
BssMI GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
BssT1I CCWWGG 3 cut(s) 312, 2417, 2723
Bst2UI CCWGG 5 cut(s) 261, 870, 902, 1980, 2385
BstAFI CTTAAG 2 cut(s) 592, 2150
BstAUI TGTACA 3 cut(s) 360, 907, 2100
BstC8I GCNNGC 6 cut(s) 165, 603, 1154, 1341, 1348, 1439
BstDEI CTNAG 5 cut(s) 729, 1275, 1377, 2187, 2715
BstDSI CCRYGG 2 cut(s) 312, 1763
BstHHI GCGC 2 cut(s) 2593, 2608
BstKTI GATC 6 cut(s) 861, 1333, 1988, 2257, 2538, 2653
BstMAI GTCTC 1 cut(s) 2584
BstMBI GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
BstMWI GCNNNNNNNGC 6 cut(s) 23, 1162, 1183, 1639, 2612, 2725
BstNI CCWGG 5 cut(s) 261, 870, 902, 1980, 2385
BstNSI RCATGY 3 cut(s) 437, 1350, 1441
BstSCI CCNGG 5 cut(s) 259, 868, 900, 1978, 2383
BstSFI CTRYAG 5 cut(s) 1248, 1698, 2024, 2574, 2616
BstV1I GCAGC 8 cut(s) 487, 610, 617, 1330, 1570, 1993, 2602, 2630
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 2650
BstYI RGATCY 1 cut(s) 2650
Bsu36I CCTNAGG 1 cut(s) 1275
BsuI GTATCC 1 cut(s) 2714
BsuRI GGCC 7 cut(s) 124, 430, 1165, 1273, 1975, 2032, 2473
BtgI CCRYGG 2 cut(s) 312, 1763
BtsI GCAGTG 2 cut(s) 380, 666
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 6 cut(s) 165, 603, 1154, 1341, 1348, 1439
CaiI CAGNNNCTG 2 cut(s) 203, 2714
CciI TCATGA 1 cut(s) 2731
CfoI GCGC 2 cut(s) 2593, 2608
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 5 cut(s) 371, 872, 1760, 1973, 2740
CseI GACGC 1 cut(s) 44
CspAI ACCGGT 1 cut(s) 683
CspCI CAANNNNNGTGG 2 cut(s) 1084, 1119
DdeI CTNAG 5 cut(s) 729, 1275, 1377, 2187, 2715
DpnI GATC 6 cut(s) 860, 1332, 1987, 2256, 2537, 2652
DpnII GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
DraIII CACNNNGTG 1 cut(s) 1806
EaeI YGGCCR 1 cut(s) 2030
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 2719
Eco130I CCWWGG 3 cut(s) 312, 2417, 2723
Eco147I AGGCCT 2 cut(s) 1165, 1273
Eco24I GRGCYC 1 cut(s) 2721
Eco47I GGWCC 4 cut(s) 371, 872, 1760, 2740
Eco53kI GAGCTC 1 cut(s) 2719
Eco57I CTGAAG 3 cut(s) 536, 1428, 2185
Eco81I CCTNAGG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 1181
EcoICRI GAGCTC 1 cut(s) 2719
EcoRI GAATTC 2 cut(s) 992, 2156
EcoRII CCWGG 5 cut(s) 259, 868, 900, 1978, 2383
EcoT14I CCWWGG 3 cut(s) 312, 2417, 2723
EcoT22I ATGCAT 2 cut(s) 571, 665
EcoT38I GRGCYC 1 cut(s) 2721
ErhI CCWWGG 3 cut(s) 312, 2417, 2723
FalI AAGNNNNNCTT 4 cut(s) 1729, 1761, 1764, 1796
FaqI GGGAC 2 cut(s) 47, 708
FauI CCCGC 1 cut(s) 926
FauNDI CATATG 1 cut(s) 2597
Fnu4HI GCNGC 9 cut(s) 501, 599, 606, 1344, 1559, 2007, 2055, 2616, 2619
FriOI GRGCYC 1 cut(s) 2721
Fsp4HI GCNGC 9 cut(s) 501, 599, 606, 1344, 1559, 2007, 2055, 2616, 2619
FspAI RTGCGCAY 1 cut(s) 2607
FspBI CTAG 8 cut(s) 120, 426, 581, 626, 1542, 2169, 2469, 2724
FspI TGCGCA 1 cut(s) 2607
GlaI GCGC 2 cut(s) 2592, 2607
GluI GCNGC 9 cut(s) 501, 599, 606, 1344, 1559, 2007, 2055, 2616, 2619
GsuI CTGGAG 2 cut(s) 431, 640
HaeIII GGCC 7 cut(s) 124, 430, 1165, 1273, 1975, 2032, 2473
HapII CCGG 1 cut(s) 684
HgaI GACGC 1 cut(s) 44
HhaI GCGC 2 cut(s) 2593, 2608
Hin6I GCGC 2 cut(s) 2591, 2606
HinP1I GCGC 2 cut(s) 2591, 2606
HincII GTYRAC 2 cut(s) 1516, 1786
HindII GTYRAC 2 cut(s) 1516, 1786
HinfI GANTC 7 cut(s) 416, 653, 1018, 1375, 2093, 2211, 2219
HpaII CCGG 1 cut(s) 684
HphI GGTGA 1 cut(s) 1084
Hpy188III TCNNGA 7 cut(s) 197, 563, 647, 657, 1277, 2673, 2732
HpyAV CCTTC 9 cut(s) 1379, 1408, 1452, 1576, 2007, 2260, 2281, 2670, 2731
HpyCH4IV ACGT 1 cut(s) 1146
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 1162, 1183, 1639, 2612, 2725
HpyF3I CTNAG 5 cut(s) 729, 1275, 1377, 2187, 2715
HpySE526I ACGT 1 cut(s) 1146
HspAI GCGC 2 cut(s) 2591, 2606
KpnI GGTACC 1 cut(s) 684
Kzo9I GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
LmnI GCTCC 7 cut(s) 100, 542, 1057, 1408, 2248, 2459, 2724
Lsp1109I GCAGC 8 cut(s) 487, 610, 617, 1330, 1570, 1993, 2602, 2630
LweI GCATC 8 cut(s) 190, 538, 556, 650, 672, 1557, 1702, 2422
MaeI CTAG 8 cut(s) 120, 426, 581, 626, 1542, 2169, 2469, 2724
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 6 cut(s) 436, 806, 813, 1294, 1798, 2686
MalI GATC 6 cut(s) 860, 1332, 1987, 2256, 2537, 2652
MbiI CCGCTC 1 cut(s) 2054
MboI GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
MfeI CAATTG 3 cut(s) 522, 1370, 2583
MflI RGATCY 1 cut(s) 2650
MhlI GDGCHC 2 cut(s) 2446, 2721
MlsI TGGCCA 1 cut(s) 2032
MluNI TGGCCA 1 cut(s) 2032
MlyI GAGTC 3 cut(s) 647, 1369, 2220
MmeI TCCRAC 4 cut(s) 83, 655, 1231, 1234
Mox20I TGGCCA 1 cut(s) 2032
Mph1103I ATGCAT 2 cut(s) 571, 665
MroXI GAANNNNTTC 2 cut(s) 1744, 1885
MscI TGGCCA 1 cut(s) 2032
MslI CAYNNNNRTG 2 cut(s) 404, 2484
Msp20I TGGCCA 1 cut(s) 2032
MspCI CTTAAG 2 cut(s) 592, 2150
MspI CCGG 1 cut(s) 684
MspR9I CCNGG 5 cut(s) 261, 870, 902, 1980, 2385
MunI CAATTG 3 cut(s) 522, 1370, 2583
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 5 cut(s) 261, 870, 902, 1980, 2385
MwoI GCNNNNNNNGC 6 cut(s) 23, 1162, 1183, 1639, 2612, 2725
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 2597
NdeII GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
NlaIV GGNNCC 5 cut(s) 16, 96, 682, 1404, 2455
NmeAIII GCCGAG 1 cut(s) 2636
NmuCI GTSAC 6 cut(s) 436, 806, 813, 1294, 1798, 2686
NsbI TGCGCA 1 cut(s) 2607
NsiI ATGCAT 2 cut(s) 571, 665
NspI RCATGY 3 cut(s) 437, 1350, 1441
OliI CACNNNNGTG 1 cut(s) 404
PaeI GCATGC 2 cut(s) 1350, 1441
PaeR7I CTCGAG 1 cut(s) 1181
PagI TCATGA 1 cut(s) 2731
PaqCI CACCTGC 1 cut(s) 17
PceI AGGCCT 2 cut(s) 1165, 1273
PciI ACATGT 1 cut(s) 433
PctI GAATGC 1 cut(s) 954
PdmI GAANNNNTTC 2 cut(s) 1744, 1885
PfeI GAWTC 4 cut(s) 416, 1018, 2093, 2219
PflFI GACNNNGTC 1 cut(s) 587
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 9 cut(s) 502, 600, 607, 1345, 1560, 2008, 2056, 2617, 2620
PleI GAGTC 3 cut(s) 647, 1369, 2219
PpsI GAGTC 3 cut(s) 647, 1369, 2219
PscI ACATGT 1 cut(s) 433
Psp124BI GAGCTC 1 cut(s) 2721
Psp6I CCWGG 5 cut(s) 259, 868, 900, 1978, 2383
PspGI CCWGG 5 cut(s) 259, 868, 900, 1978, 2383
PspN4I GGNNCC 5 cut(s) 16, 96, 682, 1404, 2455
PspPI GGNCC 5 cut(s) 371, 872, 1760, 1973, 2740
PspXI VCTCGAGB 1 cut(s) 1181
PstI CTGCAG 2 cut(s) 2578, 2620
PstNI CAGNNNCTG 2 cut(s) 203, 2714
PsuI RGATCY 1 cut(s) 2650
PsyI GACNNNGTC 1 cut(s) 587
RseI CAYNNNNRTG 2 cut(s) 404, 2484
SacI GAGCTC 1 cut(s) 2721
SatI GCNGC 9 cut(s) 501, 599, 606, 1344, 1559, 2007, 2055, 2616, 2619
Sau3AI GATC 6 cut(s) 858, 1330, 1985, 2254, 2535, 2650
Sau96I GGNCC 5 cut(s) 371, 872, 1760, 1973, 2740
ScaI AGTACT 1 cut(s) 694
SchI GAGTC 3 cut(s) 647, 1369, 2220
ScrFI CCNGG 5 cut(s) 261, 870, 902, 1980, 2385
SduI GDGCHC 2 cut(s) 2446, 2721
SfaNI GCATC 8 cut(s) 190, 538, 556, 650, 672, 1557, 1702, 2422
SfcI CTRYAG 5 cut(s) 1248, 1698, 2024, 2574, 2616
Sfr274I CTCGAG 1 cut(s) 1181
SinI GGWCC 4 cut(s) 371, 872, 1760, 2740
SlaI CTCGAG 1 cut(s) 1181
SmiMI CAYNNNNRTG 2 cut(s) 404, 2484
SmlI CTYRAG 6 cut(s) 592, 1009, 1181, 1397, 1552, 2150
SmoI CTYRAG 6 cut(s) 592, 1009, 1181, 1397, 1552, 2150
SpeI ACTAGT 1 cut(s) 1541
SphI GCATGC 2 cut(s) 1350, 1441
SseBI AGGCCT 2 cut(s) 1165, 1273
SsiI CCGC 6 cut(s) 98, 919, 1350, 2009, 2054, 2743
SspI AATATT 1 cut(s) 2407
SspMI CTAG 8 cut(s) 120, 426, 581, 626, 1542, 2169, 2469, 2724
SstI GAGCTC 1 cut(s) 2721
StuI AGGCCT 2 cut(s) 1165, 1273
StyD4I CCNGG 5 cut(s) 259, 868, 900, 1978, 2383
StyI CCWWGG 3 cut(s) 312, 2417, 2723
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 3 cut(s) 1182, 1984, 2496
TatI WGTACW 8 cut(s) 360, 692, 907, 1393, 2046, 2100, 2476, 2567
TauI GCSGC 1 cut(s) 2057
TfiI GAWTC 4 cut(s) 416, 1018, 2093, 2219
TseFI GTSAC 6 cut(s) 436, 806, 813, 1294, 1798, 2686
TseI GCWGC 8 cut(s) 500, 598, 605, 1343, 1558, 2006, 2615, 2618
Tsp45I GTSAC 6 cut(s) 436, 806, 813, 1294, 1798, 2686
TspDTI ATGAA 9 cut(s) 252, 467, 1705, 1838, 2279, 2535, 2654, 2655, 2720
TspGWI ACGGA 2 cut(s) 463, 1059
Tth111I GACNNNGTC 1 cut(s) 587
Vha464I CTTAAG 2 cut(s) 592, 2150
VpaK11BI GGWCC 4 cut(s) 371, 872, 1760, 2740
XapI RAATTY 8 cut(s) 317, 332, 761, 958, 992, 1885, 2156, 2306
XceI RCATGY 3 cut(s) 437, 1350, 1441
XhoI CTCGAG 1 cut(s) 1181
XmaJI CCTAGG 1 cut(s) 2723
XmnI GAANNNNTTC 2 cut(s) 1744, 1885
XspI CTAG 8 cut(s) 120, 426, 581, 626, 1542, 2169, 2469, 2724
ZrmI AGTACT 1 cut(s) 694
Zsp2I ATGCAT 2 cut(s) 571, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.