MD09G1115300.v1.1

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001253
Physical Location & Seq
Forward (+)
8854849 .. 8862029
7181 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1115300.v1.1.491

Sequence Viewer

Length: 2553 bp
ATGGAGGATGTAGCTAGTGCTGGAATTGCAGACGGGGAACTACAACCGGCCCCACCCTCTCCAACCATACCACCTAAATTGAAGCCAGAAGTAGTGACTCCTTCAAAATATTCTATCATGAGTAGGCGTGGAACTGGAACTACTGGGAAAGAGATAAATTTGCTTGCAAATCACTTCAAAGTTTCTGTGAATGTTCCAGATGCTGTATTTTACCAGTATTCTGTTTCTATTACTTCAGAAGATAAGAAAGTTGTTGAAGGCAAGGGAATCGGGAGAAAACTTATTGACAGGCTTTATCAAACATACTCTTCAGAACTTGGTGGTAAAAAGTTTGCATACGATGGGGAGAAAGCACTGTACACAGTGGGTCCTCTGCCACAGAACAAGCTTGAGTTCACGGTTGTTCTGGAGGAAACGTTTGCAAAGCATGAAAATGGGAGCACCAATGGGACCGAAAAACGGTCAAGACGTTCTTTTCGGTCAAGAACTTTCAACTTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAACCAATTTCTCTTGTCCTCGAGGGAGCCGATGTAGAAAATACGCAAGATTCACTTAGAGTGCTTGATATTATTCTGCGGCAGCAAGCAGCTAACAGGGGTTGCCTTCTAGTACGACAGTCATTCTTCCATGATGACTCTAGAAACTTTACTGATGTTGGAGGAGGTGTAAAAGGTGTTCGGGGATTTCATTCTAGTTTTCGTCCAACTCAGGGTGGCTTATCCTTGAATATGGATGTATCTACAACAATGATCCTAACACCTGGACCTGTGATTGATTTTCTGTTAGCTAACCAGGATGTAAGAGAACCTCGCTACATAGACTGGGTAAAGGCGAAGAGAATGCTGAAAAATATGAGGATTAATGCAAGACACCGTAACATGGAATTCAAAATAATAGGCTTAAGTGAGAAGCCATGTAATCAGCAATATTTTCCTATGAAGTTAAAAATTGGTGACAATGGCACGAGTGAGGATCAGACTGTGGAGATTACTGTTTATGAGTATTTCACTAAACACTGTGGTGTAGAAATCACCTCTTCTCAGTACATGCCATGCCTTGATGTTGGCAAGCCCAAGAGGCCTAACTATCTGCCTCTGGAGCTTTGTTCGCTAGTTTCGCTTCAGCGGTATACAAAAGCATTATCTTCAAAGCAGAGGGCATCCTTAGTTGAAAAATCAAGGCAGAAGCCTCAGGAAAGAATAAGAACTGTGACTGATGCTATGAAAAAATACCAGTATGATGATGATCCTGTGCTTGCTACATGTGGTATTTCTATAAATAAGGAATTGACTCAGGTCAAGGGCCGTGTTCTTGAGACTCCGAAGTTAAAGGTTGGTAACAGTGATGATTGCATCCCCCGTAATGGACGATGGAACTTCAACAACAAGACACTGCTAAACCCTACTCGCATTGACCGTTGGATTGTTGTCAACTTCTCTGCACGCTGTGATACCAGTCACCTCTCACGAGAGCTTATCAACTGTGGGAGGAAGAAGGGCATTATTATTGAGCGCCCCTTTACATTAATTGAGGAAGATCCACAATGCAGAAGACTGACCCCTGTTGCAAGAGTTGACAAGATGTTTGAACAGATTCTAGCTAAGCTTCCAGGCGAACCTCAATTCATTCTTTGTCTTGGAGGAATAAATTCTTTGTTGGCAATAGAGCACTCCTCACGTGTTCCTCTGGTGAAGGATACTCCTACAATGATTTTGGGGATGGATGTCTCTCATGGGTCTCCTGGTCGATCAGATATTCCTTCTGTTGCTGCAGTTGTTAGCTCTCGAAGTTGGCCACTTATTTCAAGGTATAGAGCAGCTGTTAGAACACAATCTCCTAAGATGGAGATGATTGATGCTCTATACAAACCACTGGAAAATGGAACTGATGCTGGTATTATCAGGGAACTGCTTGTGGACTTCTATAAGACGAGCAATGGACGCAAACCAACTCAGATTATTGTCTTCAGGGATGGAGTGAGCGAGTCACAATTCAATCAAGTCTTGAATATCGAGCTAGACCAAATCATAAAGGCTTACCAGCACCTCGGGGAGGTTAACGTTCCAAAGTTTACTTTGATTATTGCACAGAAGAATCATCACACAAAGCTATTTCAACAGGGTTCCCCAGATAATGTTCCAGCAGGGACAGTTGTGGACACAGAGATTGTGCATCCAAGGAATTACGATTTCTACATGTGTGCTCAAGCAGGAATGATAGGAACTTCGAGGCCAGCGCACTATCATGTCTTGGTTGATGAGATTGGATTTTCTCCCGATGACTTGCAAAACCTCATTCACTCCCTTTCTTATGTGTACCAGAGGAGTACAACTGCAATCTCTATCGTGGCGCCCATTTGTTATGCCCACCTTGCTGCAGCACAAGTGGGGCAGTTTATGAAGTTTGAGGATCTGTCAGAAACGTCATCAGGGAAAGGCAGTGTCACTTCATCAGAAAGCGTCCCGGTTCCAGAGCTCCCTAAGTTGCATGAGAACGTCCAGGGCTCAATGTTCTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

851

Amino Acids

95.06

Weight (kDa)

8.98

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 50 - 202 7.1e-30 N-terminal domain of argonaute
ArgoL1 PF08699 214 - 261 5.8e-18 Argonaute linker 1 domain
PAZ PF02170 268 - 399 3.5e-28 PAZ domain
ArgoL2 PF16488 409 - 454 8.3e-13 Argonaute linker 2 domain
Piwi PF02171 539 - 811 1.8e-88 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 548
AccB1I GGYRCC 1 cut(s) 2380
AccI GTMKAC 1 cut(s) 1160
AciI CCGC 2 cut(s) 607, 1156
AclI AACGTT 2 cut(s) 416, 2091
AclWI GGATC 5 cut(s) 775, 1011, 1271, 1562, 2448
AcoI YGGCCR 1 cut(s) 1823
AcsI RAATTY 3 cut(s) 157, 914, 1678
AcuI CTGAAG 4 cut(s) 219, 294, 1136, 1981
AcvI CACGTG 1 cut(s) 1709
AcyI GRCGYC 1 cut(s) 2381
AdeI CACNNNGTG 1 cut(s) 1478
AfaI GTAC 5 cut(s) 359, 642, 1076, 2348, 2359
AfiI CCNNNNNNNGG 5 cut(s) 459, 740, 910, 1394, 2083
AflII CTTAAG 1 cut(s) 931
AflIII ACRYGT 3 cut(s) 1292, 1708, 2226
AjnI CCWGG 5 cut(s) 790, 822, 1639, 1771, 2529
AleI CACNNNNGTG 1 cut(s) 1050
AloI GAACNNNNNNTCC 2 cut(s) 1849, 1881
Alw21I GWGCWC 4 cut(s) 443, 1701, 2236, 2508
Alw26I GTCTC 3 cut(s) 1340, 1762, 1773
AlwI GGATC 5 cut(s) 775, 1011, 1271, 1562, 2448
AlwNI CAGNNNCTG 1 cut(s) 203
Ama87I CYCGRG 2 cut(s) 548, 2078
AoxI GGCC 5 cut(s) 48, 1109, 1333, 1823, 2261
ApeKI GCWGC 7 cut(s) 512, 610, 617, 1799, 1847, 2405, 2408
ApoI RAATTY 3 cut(s) 157, 914, 1678
ArsI GACNNNNNNTTYG 4 cut(s) 459, 491, 1598, 1630
AseI ATTAAT 2 cut(s) 891, 1556
Asp700I GAANNNNTTC 2 cut(s) 1623, 1678
AspLEI GCGC 3 cut(s) 1545, 2269, 2383
AspS9I GGNCC 5 cut(s) 49, 368, 450, 794, 1333
AsuC2I CCSGG 1 cut(s) 2495
AsuHPI GGTGA 4 cut(s) 995, 1054, 1481, 1732
AvaI CYCGRG 2 cut(s) 548, 2078
AvaII GGWCC 3 cut(s) 368, 450, 794
AxyI CCTNAGG 1 cut(s) 1221
BalI TGGCCA 1 cut(s) 1825
BanI GGYRCC 1 cut(s) 2380
BanII GRGCYC 2 cut(s) 2508, 2537
BauI CACGAG 2 cut(s) 994, 1497
BbrPI CACGTG 1 cut(s) 1709
BbsI GAAGAC 2 cut(s) 1588, 1987
Bbv12I GWGCWC 4 cut(s) 443, 1701, 2236, 2508
BbvI GCAGC 7 cut(s) 499, 622, 629, 1786, 1859, 2392, 2420
BccI CCATC 5 cut(s) 335, 1395, 1744, 1867, 1997
BceAI ACGGC 1 cut(s) 1320
BcgI CGANNNNNNTGC 4 cut(s) 250, 284, 853, 887
BciT130I CCWGG 5 cut(s) 792, 824, 1641, 1773, 2531
BciVI GTATCC 1 cut(s) 1720
BcnI CCSGG 1 cut(s) 2495
BcoDI GTCTC 3 cut(s) 1340, 1762, 1773
BfaI CTAG 7 cut(s) 15, 638, 669, 723, 1142, 1628, 2048
BfmI CTRYAG 2 cut(s) 1800, 2406
BfoI RGCGCY 2 cut(s) 1546, 2384
BfrI CTTAAG 1 cut(s) 931
BfuI GTATCC 1 cut(s) 1720
BglI GCCNNNNNGGC 1 cut(s) 1108
BisI GCNGC 8 cut(s) 513, 608, 611, 618, 1800, 1848, 2406, 2409
BlpI GCTNAGC 1 cut(s) 1632
BlsI GCNGC 8 cut(s) 514, 609, 612, 619, 1801, 1849, 2407, 2410
Bme1390I CCNGG 6 cut(s) 792, 824, 1641, 1773, 2495, 2531
Bme18I GGWCC 3 cut(s) 368, 450, 794
BmeT110I CYCGRG 2 cut(s) 548, 2078
BmgT120I GGNCC 5 cut(s) 49, 368, 450, 794, 1333
BmiI GGNNCC 7 cut(s) 51, 369, 451, 556, 2155, 2382, 2499
BmrFI CCNGG 6 cut(s) 792, 824, 1641, 1773, 2495, 2531
BmrI ACTGGG 2 cut(s) 153, 862
BmsI GCATC 7 cut(s) 190, 1199, 1237, 1392, 1876, 1909, 2212
BmuI ACTGGG 2 cut(s) 153, 862
BoxI GACNNNNGTC 1 cut(s) 1325
BpiI GAAGAC 2 cut(s) 1588, 1987
BplI GAGNNNNNCTC 2 cut(s) 1688, 1720
BpmI CTGGAG 2 cut(s) 428, 1148
Bpu1102I GCTNAGC 1 cut(s) 1632
BpuEI CTTGAG 3 cut(s) 410, 1364, 2220
BpuMI CCSGG 1 cut(s) 2495
BsaAI YACGTR 1 cut(s) 1709
BsaBI GATNNNNATC 1 cut(s) 1275
BsaHI GRCGYC 1 cut(s) 2381
BsaI GGTCTC 1 cut(s) 1773
BsaJI CCNNGG 3 cut(s) 2077, 2207, 2530
BsaXI ACNNNNNCTCC 8 cut(s) 401, 431, 546, 576, 681, 711, 1849, 1879
Bsc4I CCNNNNNNNGG 5 cut(s) 459, 740, 910, 1394, 2083
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 7 cut(s) 139, 148, 214, 857, 1264, 1485, 1908
Bse21I CCTNAGG 1 cut(s) 1221
Bse3DI GCAATG 1 cut(s) 1972
Bse8I GATNNNNATC 1 cut(s) 1275
BseBI CCWGG 5 cut(s) 792, 824, 1641, 1773, 2531
BseDI CCNNGG 3 cut(s) 2077, 2207, 2530
BseGI GGATG 9 cut(s) 13, 769, 832, 1190, 1383, 1755, 1759, 2008, 2203
BseJI GATNNNNATC 1 cut(s) 1275
BseLI CCNNNNNNNGG 5 cut(s) 459, 740, 910, 1394, 2083
BseMI GCAATG 1 cut(s) 1972
BseMII CTCAG 5 cut(s) 752, 1085, 1235, 1337, 1997
BseNI ACTGG 7 cut(s) 139, 148, 214, 857, 1264, 1485, 1908
BseRI GAGGAG 3 cut(s) 705, 1693, 2368
BseXI GCAGC 7 cut(s) 499, 622, 629, 1786, 1859, 2392, 2420
BsgI GTGCAG 1 cut(s) 1455
BshFI GGCC 5 cut(s) 50, 1111, 1335, 1825, 2263
BshNI GGYRCC 1 cut(s) 2380
BsiHKAI GWGCWC 4 cut(s) 443, 1701, 2236, 2508
BsiHKCI CYCGRG 2 cut(s) 548, 2078
BsiSI CCGG 2 cut(s) 47, 2495
BslFI GGGAC 3 cut(s) 463, 2191, 2477
BslI CCNNNNNNNGG 5 cut(s) 459, 740, 910, 1394, 2083
BsmAI GTCTC 3 cut(s) 1340, 1762, 1773
BsmFI GGGAC 3 cut(s) 463, 2191, 2477
BsmI GAATGC 1 cut(s) 876
BsnI GGCC 5 cut(s) 50, 1111, 1335, 1825, 2263
Bso31I GGTCTC 1 cut(s) 1773
BsoBI CYCGRG 2 cut(s) 548, 2078
Bsp1286I GDGCHC 5 cut(s) 443, 1701, 2236, 2508, 2537
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
Bsp1720I GCTNAGC 1 cut(s) 1632
BspACI CCGC 2 cut(s) 607, 1156
BspANI GGCC 5 cut(s) 50, 1111, 1335, 1825, 2263
BspCNI CTCAG 5 cut(s) 751, 1084, 1234, 1336, 1996
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 7 cut(s) 51, 369, 451, 556, 2155, 2382, 2499
BspMAI CTGCAG 2 cut(s) 1804, 2410
BspPI GGATC 5 cut(s) 775, 1011, 1271, 1562, 2448
BspT107I GGYRCC 1 cut(s) 2380
BspTI CTTAAG 1 cut(s) 931
BspTNI GGTCTC 1 cut(s) 1773
BsrDI GCAATG 1 cut(s) 1972
BsrFI RCCGGY 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 7 cut(s) 139, 148, 214, 857, 1264, 1485, 1908
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 3 cut(s) 2077, 2207, 2530
BssMI GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
BssNAI GTATAC 1 cut(s) 1161
BssNI GRCGYC 1 cut(s) 2381
BssSI CACGAG 2 cut(s) 994, 1497
BssT1I CCWWGG 1 cut(s) 2207
Bst1107I GTATAC 1 cut(s) 1161
Bst2BI CACGAG 2 cut(s) 994, 1497
Bst2UI CCWGG 5 cut(s) 792, 824, 1641, 1773, 2531
Bst6I CTCTTC 3 cut(s) 313, 860, 1072
BstACI GRCGYC 1 cut(s) 2381
BstAFI CTTAAG 1 cut(s) 931
BstAUI TGTACA 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 1709
BstC8I GCNNGC 6 cut(s) 165, 615, 1100, 1287, 1474, 2265
BstENI CCTNNNNNAGG 1 cut(s) 2081
BstF5I GGATG 9 cut(s) 13, 769, 832, 1190, 1383, 1755, 1759, 2008, 2203
BstH2I RGCGCY 2 cut(s) 1546, 2384
BstHHI GCGC 3 cut(s) 1545, 2269, 2383
BstKTI GATC 6 cut(s) 783, 1006, 1279, 1570, 1781, 2443
BstMAI GTCTC 3 cut(s) 1340, 1762, 1773
BstMBI GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
BstMWI GCNNNNNNNGC 7 cut(s) 26, 1108, 1129, 1138, 1147, 1971, 2402
BstNI CCWGG 5 cut(s) 792, 824, 1641, 1773, 2531
BstNSI RCATGY 3 cut(s) 1081, 1296, 2230
BstPAI GACNNNNGTC 1 cut(s) 1325
BstSCI CCNGG 6 cut(s) 790, 822, 1639, 1771, 2493, 2529
BstSFI CTRYAG 2 cut(s) 1800, 2406
BstV1I GCAGC 7 cut(s) 499, 622, 629, 1786, 1859, 2392, 2420
BstV2I GAAGAC 2 cut(s) 1588, 1987
BstX2I RGATCY 2 cut(s) 1567, 2440
BstYI RGATCY 2 cut(s) 1567, 2440
BstZ17I GTATAC 1 cut(s) 1161
Bsu36I CCTNAGG 1 cut(s) 1221
BsuI GTATCC 1 cut(s) 1720
BsuRI GGCC 5 cut(s) 50, 1111, 1335, 1825, 2263
BtsCI GGATG 9 cut(s) 13, 769, 832, 1190, 1383, 1755, 1759, 2008, 2203
BtsI GCAGTG 2 cut(s) 1421, 2476
BtsIMutI CAGTG 7 cut(s) 353, 369, 1045, 1378, 1421, 1901, 2476
Cac8I GCNNGC 6 cut(s) 165, 615, 1100, 1287, 1474, 2265
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 1 cut(s) 117
CfoI GCGC 3 cut(s) 1545, 2269, 2383
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 5 cut(s) 49, 368, 450, 794, 1333
CseI GACGC 2 cut(s) 1980, 2479
Csp6I GTAC 5 cut(s) 358, 641, 1075, 2347, 2358
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviQI GTAC 5 cut(s) 358, 641, 1075, 2347, 2358
DinI GGCGCC 1 cut(s) 2382
DpnI GATC 6 cut(s) 782, 1005, 1278, 1569, 1780, 2442
DpnII GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
DraIII CACNNNGTG 1 cut(s) 1478
EaeI YGGCCR 1 cut(s) 1823
Eam1104I CTCTTC 3 cut(s) 313, 860, 1072
EarI CTCTTC 3 cut(s) 313, 860, 1072
Ecl136II GAGCTC 1 cut(s) 2506
Eco130I CCWWGG 1 cut(s) 2207
Eco147I AGGCCT 1 cut(s) 1111
Eco24I GRGCYC 2 cut(s) 2508, 2537
Eco31I GGTCTC 1 cut(s) 1773
Eco47I GGWCC 3 cut(s) 368, 450, 794
Eco53kI GAGCTC 1 cut(s) 2506
Eco57I CTGAAG 4 cut(s) 219, 294, 1136, 1981
Eco72I CACGTG 1 cut(s) 1709
Eco81I CCTNAGG 1 cut(s) 1221
Eco88I CYCGRG 2 cut(s) 548, 2078
EcoICRI GAGCTC 1 cut(s) 2506
EcoNI CCTNNNNNAGG 1 cut(s) 2081
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 5 cut(s) 790, 822, 1639, 1771, 2529
EcoT14I CCWWGG 1 cut(s) 2207
EcoT38I GRGCYC 2 cut(s) 2508, 2537
EgeI GGCGCC 1 cut(s) 2382
EheI GGCGCC 1 cut(s) 2382
ErhI CCWWGG 1 cut(s) 2207
FalI AAGNNNNNCTT 4 cut(s) 457, 489, 567, 599
FaqI GGGAC 3 cut(s) 463, 2191, 2477
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 8 cut(s) 513, 608, 611, 618, 1800, 1848, 2406, 2409
FokI GGATG 9 cut(s) 20, 776, 839, 1177, 1370, 1762, 1766, 2015, 2190
FriOI GRGCYC 2 cut(s) 2508, 2537
Fsp4HI GCNGC 8 cut(s) 513, 608, 611, 618, 1800, 1848, 2406, 2409
FspBI CTAG 7 cut(s) 15, 638, 669, 723, 1142, 1628, 2048
GlaI GCGC 3 cut(s) 1544, 2268, 2382
GluI GCNGC 8 cut(s) 513, 608, 611, 618, 1800, 1848, 2406, 2409
GsuI CTGGAG 2 cut(s) 428, 1148
HaeII RGCGCY 2 cut(s) 1546, 2384
HaeIII GGCC 5 cut(s) 50, 1111, 1335, 1825, 2263
HapII CCGG 2 cut(s) 47, 2495
HgaI GACGC 2 cut(s) 1980, 2479
HhaI GCGC 3 cut(s) 1545, 2269, 2383
Hin1I GRCGYC 1 cut(s) 2381
Hin6I GCGC 3 cut(s) 1543, 2267, 2381
HinP1I GCGC 3 cut(s) 1543, 2267, 2381
HincII GTYRAC 3 cut(s) 1462, 1606, 2089
HindII GTYRAC 3 cut(s) 1462, 1606, 2089
HindIII AAGCTT 2 cut(s) 386, 1634
HinfI GANTC 9 cut(s) 97, 267, 578, 665, 1321, 1348, 1624, 2015, 2125
HpaI GTTAAC 1 cut(s) 2089
HpaII CCGG 2 cut(s) 47, 2495
HphI GGTGA 4 cut(s) 995, 1054, 1481, 1732
Hpy188I TCNGA 9 cut(s) 238, 313, 528, 1008, 1353, 1783, 1986, 2449, 2485
HpyAV CCTTC 6 cut(s) 111, 251, 644, 1519, 1717, 1800
HpyCH4IV ACGT 6 cut(s) 416, 469, 1708, 2091, 2453, 2526
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 1108, 1129, 1138, 1147, 1971, 2402
HpySE526I ACGT 6 cut(s) 416, 469, 1708, 2091, 2453, 2526
Hsp92I GRCGYC 1 cut(s) 2381
HspAI GCGC 3 cut(s) 1543, 2267, 2381
KasI GGCGCC 1 cut(s) 2380
KspAI GTTAAC 1 cut(s) 2089
Kzo9I GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
LmnI GCTCC 4 cut(s) 438, 554, 1129, 2511
Lsp1109I GCAGC 7 cut(s) 499, 622, 629, 1786, 1859, 2392, 2420
LweI GCATC 7 cut(s) 190, 1199, 1237, 1392, 1876, 1909, 2212
MaeI CTAG 7 cut(s) 15, 638, 669, 723, 1142, 1628, 2048
MaeII ACGT 6 cut(s) 416, 469, 1708, 2091, 2453, 2526
MaeIII GTNAC 8 cut(s) 94, 905, 983, 1240, 1367, 1487, 2016, 2473
MalI GATC 6 cut(s) 782, 1005, 1278, 1569, 1780, 2442
MboI GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
MflI RGATCY 2 cut(s) 1567, 2440
MhlI GDGCHC 5 cut(s) 443, 1701, 2236, 2508, 2537
MlsI TGGCCA 1 cut(s) 1825
MluNI TGGCCA 1 cut(s) 1825
Mly113I GGCGCC 1 cut(s) 2381
MlyI GAGTC 5 cut(s) 91, 659, 1315, 1342, 2024
MmeI TCCRAC 4 cut(s) 86, 667, 758, 1430
Mox20I TGGCCA 1 cut(s) 1825
MroXI GAANNNNTTC 2 cut(s) 1623, 1678
MscI TGGCCA 1 cut(s) 1825
MseI TTAA 6 cut(s) 891, 932, 974, 1358, 1556, 2088
MslI CAYNNNNRTG 3 cut(s) 432, 1050, 2274
Msp20I TGGCCA 1 cut(s) 1825
MspA1I CMGCKG 2 cut(s) 1156, 1850
MspCI CTTAAG 1 cut(s) 931
MspI CCGG 2 cut(s) 47, 2495
MspR9I CCNGG 6 cut(s) 792, 824, 1641, 1773, 2495, 2531
Mva1269I GAATGC 1 cut(s) 876
MvaI CCWGG 5 cut(s) 792, 824, 1641, 1773, 2531
MwoI GCNNNNNNNGC 7 cut(s) 26, 1108, 1129, 1138, 1147, 1971, 2402
NarI GGCGCC 1 cut(s) 2381
NciI CCSGG 1 cut(s) 2495
NdeII GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
NlaIV GGNNCC 7 cut(s) 51, 369, 451, 556, 2155, 2382, 2499
NmuCI GTSAC 6 cut(s) 94, 983, 1240, 1487, 2016, 2473
NspI RCATGY 3 cut(s) 1081, 1296, 2230
OliI CACNNNNGTG 1 cut(s) 1050
PaeR7I CTCGAG 1 cut(s) 548
PagI TCATGA 1 cut(s) 117
PceI AGGCCT 1 cut(s) 1111
PciI ACATGT 2 cut(s) 1292, 2226
PcsI WCGNNNNNNNCGW 3 cut(s) 466, 555, 1444
PctI GAATGC 1 cut(s) 876
PdmI GAANNNNTTC 2 cut(s) 1623, 1678
PfeI GAWTC 4 cut(s) 267, 578, 1624, 2125
PkrI GCNGC 8 cut(s) 514, 609, 612, 619, 1801, 1849, 2407, 2410
PleI GAGTC 5 cut(s) 91, 659, 1315, 1342, 2023
PluTI GGCGCC 1 cut(s) 2384
PmaCI CACGTG 1 cut(s) 1709
PmlI CACGTG 1 cut(s) 1709
PpsI GAGTC 5 cut(s) 91, 659, 1315, 1342, 2023
Ppu21I YACGTR 1 cut(s) 1709
PpuMI RGGWCCY 1 cut(s) 368
PscI ACATGT 2 cut(s) 1292, 2226
PshAI GACNNNNGTC 1 cut(s) 1325
PshBI ATTAAT 2 cut(s) 891, 1556
Psp124BI GAGCTC 1 cut(s) 2508
Psp1406I AACGTT 2 cut(s) 416, 2091
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 5 cut(s) 790, 822, 1639, 1771, 2529
PspCI CACGTG 1 cut(s) 1709
PspGI CCWGG 5 cut(s) 790, 822, 1639, 1771, 2529
PspN4I GGNNCC 7 cut(s) 51, 369, 451, 556, 2155, 2382, 2499
PspPI GGNCC 5 cut(s) 49, 368, 450, 794, 1333
PspPPI RGGWCCY 1 cut(s) 368
PspXI VCTCGAGB 1 cut(s) 548
PsrI GAACNNNNNNTAC 4 cut(s) 124, 156, 690, 722
PstI CTGCAG 2 cut(s) 1804, 2410
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 2 cut(s) 1567, 2440
PvuII CAGCTG 1 cut(s) 1850
RsaI GTAC 5 cut(s) 359, 642, 1076, 2348, 2359
RsaNI GTAC 5 cut(s) 358, 641, 1075, 2347, 2358
RseI CAYNNNNRTG 3 cut(s) 432, 1050, 2274
SacI GAGCTC 1 cut(s) 2508
SaqAI TTAA 6 cut(s) 891, 932, 974, 1358, 1556, 2088
SatI GCNGC 8 cut(s) 513, 608, 611, 618, 1800, 1848, 2406, 2409
Sau3AI GATC 6 cut(s) 780, 1003, 1276, 1567, 1778, 2440
Sau96I GGNCC 5 cut(s) 49, 368, 450, 794, 1333
SchI GAGTC 5 cut(s) 91, 659, 1315, 1342, 2024
ScrFI CCNGG 6 cut(s) 792, 824, 1641, 1773, 2495, 2531
SduI GDGCHC 5 cut(s) 443, 1701, 2236, 2508, 2537
SfaNI GCATC 7 cut(s) 190, 1199, 1237, 1392, 1876, 1909, 2212
SfcI CTRYAG 2 cut(s) 1800, 2406
SfoI GGCGCC 1 cut(s) 2382
Sfr274I CTCGAG 1 cut(s) 548
SinI GGWCC 3 cut(s) 368, 450, 794
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 3 cut(s) 432, 1050, 2274
SmlI CTYRAG 5 cut(s) 389, 548, 931, 1343, 2235
SmoI CTYRAG 5 cut(s) 389, 548, 931, 1343, 2235
SseBI AGGCCT 1 cut(s) 1111
SsiI CCGC 2 cut(s) 607, 1156
SspDI GGCGCC 1 cut(s) 2380
SspI AATATT 2 cut(s) 110, 959
SspMI CTAG 7 cut(s) 15, 638, 669, 723, 1142, 1628, 2048
SstI GAGCTC 1 cut(s) 2508
StuI AGGCCT 1 cut(s) 1111
StyD4I CCNGG 6 cut(s) 790, 822, 1639, 1771, 2493, 2529
StyI CCWWGG 1 cut(s) 2207
TaiI ACGT 6 cut(s) 419, 472, 1711, 2094, 2456, 2529
TaqI TCGA 5 cut(s) 549, 1777, 1816, 2043, 2258
TaqII GACCGA 2 cut(s) 467, 468
TatI WGTACW 3 cut(s) 357, 1074, 2357
TauI GCSGC 1 cut(s) 610
TfiI GAWTC 4 cut(s) 267, 578, 1624, 2125
Tru1I TTAA 6 cut(s) 891, 932, 974, 1358, 1556, 2088
Tru9I TTAA 6 cut(s) 891, 932, 974, 1358, 1556, 2088
TscAI CASTG 7 cut(s) 360, 369, 1052, 1378, 1428, 1908, 2476
TseFI GTSAC 6 cut(s) 94, 983, 1240, 1487, 2016, 2473
TseI GCWGC 7 cut(s) 512, 610, 617, 1799, 1847, 2405, 2408
Tsp45I GTSAC 6 cut(s) 94, 983, 1240, 1487, 2016, 2473
TspDTI ATGAA 7 cut(s) 444, 707, 983, 1268, 1645, 2444, 2469
TspRI CASTG 7 cut(s) 360, 369, 1052, 1378, 1428, 1908, 2476
Vha464I CTTAAG 1 cut(s) 931
VpaK11BI GGWCC 3 cut(s) 368, 450, 794
VspI ATTAAT 2 cut(s) 891, 1556
XagI CCTNNNNNAGG 1 cut(s) 2081
XapI RAATTY 3 cut(s) 157, 914, 1678
XbaI TCTAGA 1 cut(s) 668
XceI RCATGY 3 cut(s) 1081, 1296, 2230
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 1160
XmnI GAANNNNTTC 2 cut(s) 1623, 1678
XspI CTAG 7 cut(s) 15, 638, 669, 723, 1142, 1628, 2048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.