Rroxscaffold_2G00091810

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001253
Physical Location & Seq
Forward (+)
13347928 .. 13348674
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091810.1

Sequence Viewer

Length: 312 bp
ATGCGTGTCGGAACTTCGAGGCCAGCTCACTATCACGTCTTGCTTGATGAGATTGGCTTTTCCCCTGATGACTTGCAGAACCTGATTCACTCCCTTTCTTATGTGTACCGAAGGAGCTGTACTGCAATAGCCATTGTCGCACCCATATGCTATGCGCACCTTGCTGCACCACAAGTGGGGCAGTTTATGAAGTTTGAAGATCTCTCGGAAACTTCATCAGCTAAAGGTAGTGTCACAACATCAGAGAGCATCCCAGTTCCCGAGCTCCCTAGGCTTCATAAAAAGGTCCAGGGATCCATGTTCTTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

103

Amino Acids

11.4

Weight (kDa)

6.95

Isoelectric Point (pI)

60.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 2 - 64 6.4e-21 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 156
AclWI GGATC 2 cut(s) 288, 301
AfaI GTAC 2 cut(s) 107, 121
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 1 cut(s) 197
AjiI CACGTC 1 cut(s) 37
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 4 cut(s) 26, 117, 221, 265
AluI AGCT 4 cut(s) 26, 117, 221, 265
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 2 cut(s) 288, 301
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 164
AspA2I CCTAGG 1 cut(s) 269
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 286
AvrII CCTAGG 1 cut(s) 269
BamHI GGATCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 290
BfaI CTAG 1 cut(s) 270
BglII AGATCT 1 cut(s) 199
BisI GCNGC 1 cut(s) 165
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 290
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 248
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BsaJI CCNNGG 2 cut(s) 269, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 254
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 269, 289
BseGI GGATG 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 176
BsnI GGCC 1 cut(s) 22
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 199, 293
BspANI GGCC 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 295
BspPI GGATC 2 cut(s) 288, 301
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 269, 289
BssMI GATC 2 cut(s) 199, 293
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 24
BstF5I GGATG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 2 cut(s) 202, 296
BstMBI GATC 2 cut(s) 199, 293
BstMWI GCNNNNNNNGC 3 cut(s) 137, 161, 271
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 199, 293
BstYI RGATCY 2 cut(s) 199, 293
BsuRI GGCC 1 cut(s) 22
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 24
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 286
Csp6I GTAC 2 cut(s) 106, 120
CviAII CATG 1 cut(s) 298
CviJI RGCY 8 cut(s) 22, 26, 57, 117, 131, 221, 265, 274
CviKI_1 RGCY 8 cut(s) 22, 26, 57, 117, 131, 221, 265, 274
CviQI GTAC 2 cut(s) 106, 120
DpnI GATC 2 cut(s) 201, 295
DpnII GATC 2 cut(s) 199, 293
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 1 cut(s) 301
FaiI YATR 7 cut(s) 102, 146, 148, 153, 188, 279, 299
FatI CATG 1 cut(s) 297
FauNDI CATATG 1 cut(s) 146
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 165
FspAI RTGCGCAY 1 cut(s) 156
FspBI CTAG 1 cut(s) 270
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 22
HhaI GCGC 1 cut(s) 157
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 3 cut(s) 11, 208, 244
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 1 cut(s) 105
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 3 cut(s) 76, 125, 167
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 161, 271
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 155
Kzo9I GATC 2 cut(s) 199, 293
LmnI GCTCC 2 cut(s) 114, 270
LpnPI CCDG 6 cut(s) 36, 78, 95, 267, 275, 302
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 201, 295
MboI GATC 2 cut(s) 199, 293
MboII GAAGA 2 cut(s) 209, 295
MflI RGATCY 2 cut(s) 199, 293
MhlI GDGCHC 1 cut(s) 267
MnlI CCTC 1 cut(s) 12
MslI CAYNNNNRTG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 137, 161, 271
NdeI CATATG 1 cut(s) 146
NdeII GATC 2 cut(s) 199, 293
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 232
NsbI TGCGCA 1 cut(s) 156
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 199, 293
RsaI GTAC 2 cut(s) 107, 121
RsaNI GTAC 2 cut(s) 106, 120
RseI CAYNNNNRTG 1 cut(s) 145
SacI GAGCTC 1 cut(s) 267
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 2 cut(s) 199, 293
Sau96I GGNCC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 290
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 28, 39, 84, 119, 162, 223, 229, 267, 288
SfaNI GCATC 1 cut(s) 258
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 145
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 269
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 17
TatI WGTACW 1 cut(s) 119
TfiI GAWTC 1 cut(s) 85
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 3 cut(s) 203, 204, 266
VpaK11BI GGWCC 1 cut(s) 286
XmaJI CCTAGG 1 cut(s) 269
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.