Rw2G043900

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001253
Physical Location & Seq
Reverse (-)
70433017 .. 70439491
6475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043900.1

Sequence Viewer

Length: 2709 bp
ATGGTGGATGTAGCTGTTGCTGGTGCTGGGAAGGCAGGTGGGGAGCCAGATTTGATCCCACCACCTCCTCCAACCATTCCGCCCAATGTGAAGCCAGAAGTCCTGAGTTCTCCCAAGTACTCTATTATGAGTAGGCGTGGTACTGGAACAACTGGGAGACGCATACAGTTGCTCGCAAACCACTTCAAAGTCACTGTGAAAGCTCCAGATGCTGTATTCTACCAGTATTCTGTTCATATTACTTCTGAAGACAAGAGGGCCGTTGAAGGCAAGGGAATTGGCAGAAAACTGGTTGAAAGGCTCTATCAAACATATTCTTCTGAACTTGCTGGTAAGAAGTTTGCGTATGATGGAGAGAAAGCTCTGTATACAGTTGGTCCTCTGCCACAGAACAAGCTTGAGTTTACAGTTGTGCTGGAGGAGACATTTGCAAAGAGTGAAAGTGGGAGTCCCAGTGAAGTGGCTGAAAAACGGTCAAGACGATCTCTTCAGTCAAAAACTTTTACCGTAGAGTTAAGTTATGCTGCCAAAATACCTCTGAAGTCAATTGCTCTTGCCCTCAAAGGAGCTGATGTCGACAATACTCAGGATGCATTGAGAGTGCTTGATATTATTCTACGGCAGCAAGCAGCTAACATGGGTTGCCTTCTGGTACGACAGTCATTCTTTCATGATGACTCCAGGAACTTTACTGATGTTGGGGCAGGTGTGATTGGTGTTCGGGGATTTCATTCCAGTTTCCGTCCAACTCAGGGTGGTTTGTCCTTGAATATGGATGTTTCTACAACAATGATCATGACTCCTGGACCGGTAATAGATTTTCTGATTGCCAACCAGGGTGTGCGAGAACCCCGCAACATTGACTGGGTAAAGGCCAAGAGAATGCTGAAAAACATGAGGATTAAGACGAGACACCGTAACATGGAATTTAAAATAATAGGCATCAGCGAGAAGCCCTGCAATCTGCAATACTTTCCTATGAAGTTAAAGCCTGGTGATGGTGCAAGTGAAGGGGAGACTGCAGAGGTTACTGTGTTTGAGTATTTCACCAAACACTGTGGCATTGAACTCACTTACTCTGCATACCTGCCATGCCTTGATGTTGGCAAACCAAAAAGGCCCAACTATCTGCCTCTGGAGCTCTGTGCGCTTGTTTCCTTACAACGGTATACGAAAGCATTATCTTCACAGCAGAGGGCATCCTTAGTCGAAAAATCAAGGCAGAAGCCTCAGGATAGAATACGAACTGTGACTGATGCTGTATGCAAATACCAGTATGATAGTGATCCTGTGCTTACTACATGTGGTATTTCTATAGAGAAGCAATTAACTCAGTTTGAAGGGCGCGTTCTTGATACTCCGAAGTTAAAGGTAGGTAACAGTGAGGATTGCACCCCTTTTAAGGGACGATGGAACTTCAACAACAAGACACTTTTGAACCCCACTCGCATTGACCGCTGGATTGTTGTCAACTTCTCTGCACGCTGTGATACCAGCCATATCTCAAGGGAGCTTATCAACAGTGGGAGGAAAAAGGGCATTCTTATTGAGCGTCCCTATACGCTGCTTGAGGAAGAATCACAGTCTAGAAGACAAAGCCCCGTTATAAGAGTTGAAAAGATGTTTGAACAAATTCAAACTAAGCTTCCAAGTCCACCTGACTTCATCCTTTGTGTTTTACCAGAGAGGAAAAATTCTGATATTTATGGGCCTTGGAAGAAAAAGAGTCTCAGTGATTTCGGTATTGTCACACAATGCATTTCCCCTTCCAAGATTAATGATCAGTACCTTACCAATGTGCTTCTGAAAATCAATTCTAAGCTTGGAGGAATAAATTCTTTGTTGGCGCTAGAACATTCCTCACGTGTCCCTTTGATAAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGTCTCCTGGTCGATCAGACATTCCTTCTGTTGCTGCCGTTGTAGGCTCTCGAAGTTGGCCACTTATTTCAAGGTACAGAGCAGCTGTTAGAACACAATCTCCTAAGGTGGAAATGATTGATGCTCTTTACAAACCACTGGCAAATGGAAAAGATGATGGTATTATCAGGGAACTGCTTGTCGACTTCTATAAGACAAGCGGTGGACGTAAACCAACTCAGATTATTGTCTTCAGGGATGGAGTAAGTGAATCACAGTTTAATCAAGTCCTGAACATTGAGCTGGATCAAATCGTAAAGGCTTACCAGCACCTAGGGGAGGCTAATATTCCAAAGTTTACTGTGATTGTGGCTCAGAAGAATCATCACACAAAGCTATTCCAAACTAATTCCCCTGATAATGTTCCACCAGGGACTGTTGTTGACACCAAAATTGTGCATCCAAGGAATTATGATTTCTACATGTGTGCTCAGGCAGGCATGATTGGAACTTCCAGGCCAGCTCACTATCATGTCTTGCTTGATGAGATTGGCTTTTCCCCTGATGACTTGCAAAACCTGATCCACTCCCTTTCTTATGTGTACCAGAGAAGTACAACTGCAATCTCTATTGTGGCGCCCATATGCTACGCGCACCTTGCTGCAGCACAAGTGGGGCAGTTCATGAAGTTTGAAGATCTCTCGGACACCTCATCGGGGAAGGGCAGTGTCACTTCATCAGAGAGCATCCCAGTTCAAGAGCTTCCCAGGCTTCATGAGAAGGTCCAGGGCTCCATGTTCTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

902

Amino Acids

100.45

Weight (kDa)

9.25

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 53 - 206 2.7e-31 N-terminal domain of argonaute
ArgoL1 PF08699 218 - 265 2.6e-17 Argonaute linker 1 domain
PAZ PF02170 272 - 402 2e-29 PAZ domain
ArgoL2 PF16488 412 - 457 2.1e-09 Argonaute linker 2 domain
Piwi PF02171 555 - 863 4.4e-107 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
AarI CACCTGC 2 cut(s) 26, 695
Acc36I ACCTGC 3 cut(s) 26, 695, 1095
AccB1I GGYRCC 1 cut(s) 2536
AccI GTMKAC 4 cut(s) 368, 576, 1169, 2103
AccII CGCG 2 cut(s) 1347, 2552
AciI CCGC 4 cut(s) 80, 853, 1456, 2121
AclWI GGATC 4 cut(s) 49, 1280, 2214, 2476
AcoI YGGCCR 1 cut(s) 1979
AcsI RAATTY 4 cut(s) 926, 1632, 1693, 1834
AcuI CTGAAG 4 cut(s) 267, 473, 560, 2137
AcvI CACGTG 1 cut(s) 1865
AcyI GRCGYC 1 cut(s) 2537
AdeI CACNNNGTG 1 cut(s) 1487
AfaI GTAC 7 cut(s) 119, 142, 654, 1787, 1997, 2504, 2515
AfiI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2239, 2616
AflIII ACRYGT 3 cut(s) 1301, 1864, 2382
AgeI ACCGGT 1 cut(s) 808
AjnI CCWGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
AloI GAACNNNNNNTCC 2 cut(s) 2005, 2037
Alw21I GWGCWC 2 cut(s) 1143, 2392
Alw26I GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1929
AlwI GGATC 4 cut(s) 49, 1280, 2214, 2476
AlwNI CAGNNNCTG 2 cut(s) 212, 2336
AoxI GGCC 6 cut(s) 258, 873, 1118, 1709, 1979, 2417
ApeKI GCWGC 8 cut(s) 524, 622, 629, 1564, 1955, 2003, 2561, 2564
ApoI RAATTY 4 cut(s) 926, 1632, 1693, 1834
AseI ATTAAT 1 cut(s) 1776
AsiGI ACCGGT 1 cut(s) 808
Asp700I GAANNNNTTC 2 cut(s) 1632, 1834
AspA2I CCTAGG 1 cut(s) 2233
AspLEI GCGC 5 cut(s) 1150, 1347, 1849, 2539, 2554
AspS9I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2683
AsuHPI GGTGA 2 cut(s) 1007, 1039
AvaII GGWCC 3 cut(s) 377, 806, 2683
AvrII CCTAGG 1 cut(s) 2233
AxyI CCTNAGG 2 cut(s) 1230, 2025
BaeI ACNNNNGTAYC 2 cut(s) 644, 677
BalI TGGCCA 1 cut(s) 1981
BanI GGYRCC 1 cut(s) 2536
BanII GRGCYC 2 cut(s) 1143, 2693
BbrPI CACGTG 1 cut(s) 1865
BbsI GAAGAC 3 cut(s) 255, 1597, 2143
Bbv12I GWGCWC 2 cut(s) 1143, 2392
BbvI GCAGC 8 cut(s) 511, 634, 641, 1551, 1942, 2015, 2548, 2576
BccI CCATC 6 cut(s) 344, 992, 1404, 1900, 2072, 2153
BceAI ACGGC 3 cut(s) 245, 635, 1943
BciT130I CCWGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
BclI TGATCA 2 cut(s) 792, 1780
BcoDI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1929
BfaI CTAG 3 cut(s) 1587, 1850, 2234
BfmI CTRYAG 3 cut(s) 1020, 1314, 2562
BfoI RGCGCY 2 cut(s) 1850, 2540
BfuAI ACCTGC 3 cut(s) 26, 695, 1095
BglII AGATCT 1 cut(s) 2596
BisI GCNGC 8 cut(s) 525, 623, 630, 1565, 1956, 2004, 2562, 2565
BlnI CCTAGG 1 cut(s) 2233
BlsI GCNGC 8 cut(s) 526, 624, 631, 1566, 1957, 2005, 2563, 2566
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
Bme18I GGWCC 3 cut(s) 377, 806, 2683
BmgT120I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2683
BmiI GGNNCC 3 cut(s) 45, 2538, 2692
BmrFI CCNGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
BmrI ACTGGG 4 cut(s) 162, 447, 874, 2645
BmsI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2032, 2368, 2655
BmuI ACTGGG 4 cut(s) 162, 447, 874, 2645
BpiI GAAGAC 3 cut(s) 255, 1597, 2143
BpmI CTGGAG 4 cut(s) 189, 437, 664, 1157
Bpu10I CCTNAGC 1 cut(s) 2391
BpuEI CTTGAG 3 cut(s) 419, 1489, 1589
BsaAI YACGTR 1 cut(s) 1865
BsaBI GATNNNNATC 1 cut(s) 1284
BsaHI GRCGYC 1 cut(s) 2537
BsaI GGTCTC 1 cut(s) 1929
BsaJI CCNNGG 7 cut(s) 835, 1712, 2233, 2330, 2363, 2666, 2686
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 6 cut(s) 439, 469, 558, 588, 2005, 2035
Bsc4I CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2239, 2616
Bse118I RCCGGY 1 cut(s) 808
Bse21I CCTNAGG 2 cut(s) 1230, 2025
Bse8I GATNNNNATC 1 cut(s) 1284
BseBI CCWGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
BseDI CCNNGG 7 cut(s) 835, 1712, 2233, 2330, 2363, 2666, 2686
BseJI GATNNNNATC 1 cut(s) 1284
BseLI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2239, 2616
BseMII CTCAG 9 cut(s) 95, 599, 764, 1244, 1346, 1744, 2153, 2288, 2405
BseRI GAGGAG 2 cut(s) 57, 434
BseXI GCAGC 8 cut(s) 511, 634, 641, 1551, 1942, 2015, 2548, 2576
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 1464
Bsh1236I CGCG 2 cut(s) 1347, 2552
BshFI GGCC 6 cut(s) 260, 875, 1120, 1711, 1981, 2419
BshNI GGYRCC 1 cut(s) 2536
BshTI ACCGGT 1 cut(s) 808
BsiHKAI GWGCWC 2 cut(s) 1143, 2392
BsiSI CCGG 1 cut(s) 809
BslFI GGGAC 5 cut(s) 435, 1419, 1539, 1853, 2347
BslI CCNNNNNNNGG 8 cut(s) 752, 922, 998, 1164, 1402, 1403, 2239, 2616
BsmAI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1929
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 5 cut(s) 435, 1419, 1539, 1853, 2347
BsmI GAATGC 2 cut(s) 888, 1539
BsnI GGCC 6 cut(s) 260, 875, 1120, 1711, 1981, 2419
Bso31I GGTCTC 1 cut(s) 1929
Bsp1286I GDGCHC 3 cut(s) 1143, 2392, 2693
Bsp143I GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
BspACI CCGC 4 cut(s) 80, 853, 1456, 2121
BspANI GGCC 6 cut(s) 260, 875, 1120, 1711, 1981, 2419
BspCNI CTCAG 9 cut(s) 96, 598, 763, 1243, 1345, 1743, 2152, 2287, 2404
BspFNI CGCG 2 cut(s) 1347, 2552
BspHI TCATGA 4 cut(s) 670, 795, 2583, 2674
BspLI GGNNCC 3 cut(s) 45, 2538, 2692
BspMAI CTGCAG 2 cut(s) 1024, 2566
BspMI ACCTGC 3 cut(s) 26, 695, 1095
BspPI GGATC 4 cut(s) 49, 1280, 2214, 2476
BspT107I GGYRCC 1 cut(s) 2536
BspTNI GGTCTC 1 cut(s) 1929
BsrFI RCCGGY 1 cut(s) 808
BssAI RCCGGY 1 cut(s) 808
BssECI CCNNGG 7 cut(s) 835, 1712, 2233, 2330, 2363, 2666, 2686
BssMI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
BssNAI GTATAC 2 cut(s) 369, 1170
BssNI GRCGYC 1 cut(s) 2537
BssT1I CCWWGG 3 cut(s) 1712, 2233, 2363
Bst1107I GTATAC 2 cut(s) 369, 1170
Bst2UI CCWGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 2537
BstBAI YACGTR 1 cut(s) 1865
BstC8I GCNNGC 5 cut(s) 174, 627, 1483, 2398, 2421
BstENI CCTNNNNNAGG 1 cut(s) 2237
BstFNI CGCG 2 cut(s) 1347, 2552
BstH2I RGCGCY 2 cut(s) 1850, 2540
BstHHI GCGC 5 cut(s) 1150, 1347, 1849, 2539, 2554
BstKTI GATC 9 cut(s) 57, 485, 795, 1288, 1783, 1937, 2209, 2484, 2599
BstMAI GTCTC 6 cut(s) 151, 416, 904, 1010, 1733, 1929
BstMBI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
BstMWI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2558, 2668
BstNI CCWGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
BstNSI RCATGY 2 cut(s) 1305, 2386
BstSCI CCNGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
BstSFI CTRYAG 3 cut(s) 1020, 1314, 2562
BstUI CGCG 2 cut(s) 1347, 2552
BstV1I GCAGC 8 cut(s) 511, 634, 641, 1551, 1942, 2015, 2548, 2576
BstV2I GAAGAC 3 cut(s) 255, 1597, 2143
BstX2I RGATCY 1 cut(s) 2596
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 2596
BstZ17I GTATAC 2 cut(s) 369, 1170
Bsu36I CCTNAGG 2 cut(s) 1230, 2025
BsuRI GGCC 6 cut(s) 260, 875, 1120, 1711, 1981, 2419
BtsI GCAGTG 1 cut(s) 2632
BtsIMutI CAGTG 8 cut(s) 192, 460, 1054, 1387, 1528, 1738, 2057, 2632
BveI ACCTGC 3 cut(s) 26, 695, 1095
Cac8I GCNNGC 5 cut(s) 174, 627, 1483, 2398, 2421
CaiI CAGNNNCTG 2 cut(s) 212, 2336
CciI TCATGA 4 cut(s) 670, 795, 2583, 2674
CfoI GCGC 5 cut(s) 1150, 1347, 1849, 2539, 2554
Cfr10I RCCGGY 1 cut(s) 808
Cfr13I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2683
CseI GACGC 2 cut(s) 168, 1541
Csp6I GTAC 7 cut(s) 118, 141, 653, 1786, 1996, 2503, 2514
CspAI ACCGGT 1 cut(s) 808
CspCI CAANNNNNGTGG 4 cut(s) 1039, 1074, 1432, 1467
CviQI GTAC 7 cut(s) 118, 141, 653, 1786, 1996, 2503, 2514
DinI GGCGCC 1 cut(s) 2538
DpnI GATC 9 cut(s) 56, 484, 794, 1287, 1782, 1936, 2208, 2483, 2598
DpnII GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
DraI TTTAAA 1 cut(s) 931
DraIII CACNNNGTG 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 1979
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 1141
Eco130I CCWWGG 3 cut(s) 1712, 2233, 2363
Eco24I GRGCYC 2 cut(s) 1143, 2693
Eco31I GGTCTC 1 cut(s) 1929
Eco47I GGWCC 3 cut(s) 377, 806, 2683
Eco53kI GAGCTC 1 cut(s) 1141
Eco57I CTGAAG 4 cut(s) 267, 473, 560, 2137
Eco72I CACGTG 1 cut(s) 1865
Eco81I CCTNAGG 2 cut(s) 1230, 2025
EcoICRI GAGCTC 1 cut(s) 1141
EcoNI CCTNNNNNAGG 1 cut(s) 2237
EcoRII CCWGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
EcoT14I CCWWGG 3 cut(s) 1712, 2233, 2363
EcoT22I ATGCAT 2 cut(s) 595, 1760
EcoT38I GRGCYC 2 cut(s) 1143, 2693
EgeI GGCGCC 1 cut(s) 2538
EheI GGCGCC 1 cut(s) 2538
ErhI CCWWGG 3 cut(s) 1712, 2233, 2363
Esp3I CGTCTC 1 cut(s) 151
FaqI GGGAC 5 cut(s) 435, 1419, 1539, 1853, 2347
FauI CCCGC 1 cut(s) 860
FauNDI CATATG 1 cut(s) 2543
FbaI TGATCA 2 cut(s) 792, 1780
FblI GTMKAC 4 cut(s) 368, 576, 1169, 2103
Fnu4HI GCNGC 8 cut(s) 525, 623, 630, 1565, 1956, 2004, 2562, 2565
FriOI GRGCYC 2 cut(s) 1143, 2693
Fsp4HI GCNGC 8 cut(s) 525, 623, 630, 1565, 1956, 2004, 2562, 2565
FspBI CTAG 3 cut(s) 1587, 1850, 2234
GlaI GCGC 5 cut(s) 1149, 1346, 1848, 2538, 2553
GluI GCNGC 8 cut(s) 525, 623, 630, 1565, 1956, 2004, 2562, 2565
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 4 cut(s) 189, 437, 664, 1157
HaeII RGCGCY 2 cut(s) 1850, 2540
HaeIII GGCC 6 cut(s) 260, 875, 1120, 1711, 1981, 2419
HapII CCGG 1 cut(s) 809
HgaI GACGC 2 cut(s) 168, 1541
HhaI GCGC 5 cut(s) 1150, 1347, 1849, 2539, 2554
Hin1I GRCGYC 1 cut(s) 2537
Hin6I GCGC 5 cut(s) 1148, 1345, 1847, 2537, 2552
HinP1I GCGC 5 cut(s) 1148, 1345, 1847, 2537, 2552
HincII GTYRAC 4 cut(s) 577, 1471, 2104, 2344
HindII GTYRAC 4 cut(s) 577, 1471, 2104, 2344
HindIII AAGCTT 3 cut(s) 395, 1643, 1820
HinfI GANTC 7 cut(s) 448, 677, 799, 1577, 1726, 2171, 2281
HpaII CCGG 1 cut(s) 809
HphI GGTGA 2 cut(s) 1007, 1039
HpyAV CCTTC 9 cut(s) 25, 260, 656, 1004, 1334, 1776, 1956, 2614, 2674
HpyCH4IV ACGT 2 cut(s) 1864, 2128
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2558, 2668
HpySE526I ACGT 2 cut(s) 1864, 2128
Hsp92I GRCGYC 1 cut(s) 2537
HspAI GCGC 5 cut(s) 1148, 1345, 1847, 2537, 2552
KasI GGCGCC 1 cut(s) 2536
Ksp22I TGATCA 2 cut(s) 792, 1780
Kzo9I GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
LmnI GCTCC 6 cut(s) 43, 208, 566, 1138, 1510, 2696
Lsp1109I GCAGC 8 cut(s) 511, 634, 641, 1551, 1942, 2015, 2548, 2576
LweI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2032, 2368, 2655
MaeI CTAG 3 cut(s) 1587, 1850, 2234
MaeII ACGT 2 cut(s) 1864, 2128
MaeIII GTNAC 7 cut(s) 190, 917, 1027, 1249, 1376, 1747, 2629
MalI GATC 9 cut(s) 56, 484, 794, 1287, 1782, 1936, 2208, 2483, 2598
MboI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 2596
MhlI GDGCHC 3 cut(s) 1143, 2392, 2693
MlsI TGGCCA 1 cut(s) 1981
MluNI TGGCCA 1 cut(s) 1981
Mly113I GGCGCC 1 cut(s) 2537
MlyI GAGTC 4 cut(s) 457, 671, 793, 1735
MmeI TCCRAC 2 cut(s) 95, 770
Mox20I TGGCCA 1 cut(s) 1981
Mph1103I ATGCAT 2 cut(s) 595, 1760
MroXI GAANNNNTTC 2 cut(s) 1632, 1834
MscI TGGCCA 1 cut(s) 1981
MseI TTAA 9 cut(s) 515, 903, 930, 986, 1328, 1367, 1401, 1776, 2181
MslI CAYNNNNRTG 1 cut(s) 2430
Msp20I TGGCCA 1 cut(s) 1981
MspA1I CMGCKG 2 cut(s) 1458, 2006
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 2 cut(s) 888, 1539
MvaI CCWGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
MvnI CGCG 2 cut(s) 1347, 2552
MwoI GCNNNNNNNGC 7 cut(s) 32, 209, 1138, 1147, 1455, 2558, 2668
NarI GGCGCC 1 cut(s) 2537
NdeI CATATG 1 cut(s) 2543
NdeII GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
NlaIV GGNNCC 3 cut(s) 45, 2538, 2692
NmuCI GTSAC 4 cut(s) 190, 1249, 1747, 2629
NsiI ATGCAT 2 cut(s) 595, 1760
NspI RCATGY 2 cut(s) 1305, 2386
PagI TCATGA 4 cut(s) 670, 795, 2583, 2674
PaqCI CACCTGC 2 cut(s) 26, 695
PciI ACATGT 2 cut(s) 1301, 2382
PcsI WCGNNNNNNNCGW 1 cut(s) 478
PctI GAATGC 2 cut(s) 888, 1539
PdmI GAANNNNTTC 2 cut(s) 1632, 1834
PfeI GAWTC 3 cut(s) 1577, 2171, 2281
PfoI TCCNGGA 2 cut(s) 680, 802
PinAI ACCGGT 1 cut(s) 808
PkrI GCNGC 8 cut(s) 526, 624, 631, 1566, 1957, 2005, 2563, 2566
PleI GAGTC 4 cut(s) 456, 671, 793, 1734
PluTI GGCGCC 1 cut(s) 2540
PmaCI CACGTG 1 cut(s) 1865
PmlI CACGTG 1 cut(s) 1865
PpsI GAGTC 4 cut(s) 456, 671, 793, 1734
Ppu21I YACGTR 1 cut(s) 1865
PscI ACATGT 2 cut(s) 1301, 2382
PshBI ATTAAT 1 cut(s) 1776
PsiI TTATAA 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 1143
Psp6I CCWGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
PspCI CACGTG 1 cut(s) 1865
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
PspN4I GGNNCC 3 cut(s) 45, 2538, 2692
PspPI GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2683
PstI CTGCAG 2 cut(s) 1024, 2566
PstNI CAGNNNCTG 2 cut(s) 212, 2336
PsuI RGATCY 1 cut(s) 2596
PvuII CAGCTG 1 cut(s) 2006
RsaI GTAC 7 cut(s) 119, 142, 654, 1787, 1997, 2504, 2515
RsaNI GTAC 7 cut(s) 118, 141, 653, 1786, 1996, 2503, 2514
RseI CAYNNNNRTG 1 cut(s) 2430
SacI GAGCTC 1 cut(s) 1143
SalI GTCGAC 2 cut(s) 575, 2102
SaqAI TTAA 9 cut(s) 515, 903, 930, 986, 1328, 1367, 1401, 1776, 2181
SatI GCNGC 8 cut(s) 525, 623, 630, 1565, 1956, 2004, 2562, 2565
Sau3AI GATC 9 cut(s) 54, 482, 792, 1285, 1780, 1934, 2206, 2481, 2596
Sau96I GGNCC 6 cut(s) 258, 377, 806, 1119, 1709, 2683
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 4 cut(s) 457, 671, 793, 1735
ScrFI CCNGG 9 cut(s) 682, 804, 836, 993, 1929, 2331, 2416, 2668, 2687
SduI GDGCHC 3 cut(s) 1143, 2392, 2693
SfaNI GCATC 8 cut(s) 199, 580, 951, 1208, 1246, 2032, 2368, 2655
SfcI CTRYAG 3 cut(s) 1020, 1314, 2562
SfoI GGCGCC 1 cut(s) 2538
SinI GGWCC 3 cut(s) 377, 806, 2683
SmiMI CAYNNNNRTG 1 cut(s) 2430
SmlI CTYRAG 3 cut(s) 398, 1504, 1568
SmoI CTYRAG 3 cut(s) 398, 1504, 1568
SsiI CCGC 4 cut(s) 80, 853, 1456, 2121
SspDI GGCGCC 1 cut(s) 2536
SspI AATATT 1 cut(s) 2248
SspMI CTAG 3 cut(s) 1587, 1850, 2234
SstI GAGCTC 1 cut(s) 1143
StyD4I CCNGG 9 cut(s) 680, 802, 834, 991, 1927, 2329, 2414, 2666, 2685
StyI CCWWGG 3 cut(s) 1712, 2233, 2363
TaiI ACGT 2 cut(s) 1867, 2131
TaqI TCGA 5 cut(s) 576, 1209, 1933, 1972, 2103
TatI WGTACW 2 cut(s) 117, 2513
TfiI GAWTC 3 cut(s) 1577, 2171, 2281
Tru1I TTAA 9 cut(s) 515, 903, 930, 986, 1328, 1367, 1401, 1776, 2181
Tru9I TTAA 9 cut(s) 515, 903, 930, 986, 1328, 1367, 1401, 1776, 2181
TscAI CASTG 8 cut(s) 199, 460, 1061, 1387, 1528, 1738, 2064, 2632
TseFI GTSAC 4 cut(s) 190, 1249, 1747, 2629
TseI GCWGC 8 cut(s) 524, 622, 629, 1564, 1955, 2003, 2561, 2564
Tsp45I GTSAC 4 cut(s) 190, 1249, 1747, 2629
TspDTI ATGAA 9 cut(s) 224, 659, 719, 995, 1654, 2572, 2600, 2625, 2663
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 8 cut(s) 199, 460, 1061, 1387, 1528, 1738, 2064, 2632
VpaK11BI GGWCC 3 cut(s) 377, 806, 2683
VspI ATTAAT 1 cut(s) 1776
XagI CCTNNNNNAGG 1 cut(s) 2237
XapI RAATTY 4 cut(s) 926, 1632, 1693, 1834
XbaI TCTAGA 1 cut(s) 1586
XceI RCATGY 2 cut(s) 1305, 2386
XmaJI CCTAGG 1 cut(s) 2233
XmiI GTMKAC 4 cut(s) 368, 576, 1169, 2103
XmnI GAANNNNTTC 2 cut(s) 1632, 1834
XspI CTAG 3 cut(s) 1587, 1850, 2234
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 595, 1760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.