Rroxscaffold_2G00091770

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001253
Physical Location & Seq
Forward (+)
13308849 .. 13316498
7650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091770.1

Sequence Viewer

Length: 2508 bp
ATGGTGGATGTAGCTGATGCTGGTGCTGGGAAGGCAGGTGGGGAACCAGATTTGATCCCACCACCTCCTCCAACCATTCCGCCTAATGTGAAGCCAGAAGTCCTGAGTTCTCCCAAGTATTCTATTATGAGTAGGCGTGGTACTGGAACAACTGGGAGACGCATACAATTGCTCGCAAACCACTTCAAAGTCACTGTGAAAGCTCCAGATGCTGTATTCTACCAGTATTCTATTCCTCTTTATAGTCTCTTATACAAGAATCCATATATTTTCTCAACTGGGTCTGTTCTCTTCTTTTGTGTTAGTGAAAGTGGGAGTCCCAGTGAAGTGGCTGAAAAACGGTCAAGACGATCTCTTCAGTCAAAAACTTTTACCGTAGAGTTAAGTTATGCTGCCAAAATACCTCTGAAGTCAATTGCTCTTGCCCTCAAAGGAGCTGATGTCGACAATACTCAGGATGCATTGAGAGTGCTTGATATTATTCTACGGCAGCAAGCAGCTAACATGGGTTGCCTTCTGGTACGACAGTCATTCTTTCACGATGACTCCAGGAACTTTACTGATGTTGGGGCAGGTGTGATTGGTGTTCGGGGATTTCATTCCAGTTTCCGTCCAACTCAGGGTGGTTTGTCCTTGAATATGGATGTATCTACAACAATGATCATGACTCCTGGACCGGTAATAGATTTTCTGATAGCCAACCAGGGTGTGCGAGAACCCCGCAACATTGACTGGGTAAAGGCCAAGAGAATGCTGAAAAACATGAGGATTAAGACAAGACACCGTAACATGGAATTTAAAATAATAGGCATCAGCGAGAAGCCCTGCAATCTGCAATACTTTCCTATGAAGTTAAAGCCTGGTGATGGTGCAAGTGAAGGGGAGACTGCAGAGGTTACTGTGTTTGAGTATTTCACCAAACACTGTGGCATTGAACTCACTTACTCTGCATACCTGCCATGCCTTGATGTTGGCAAACCAAAAAGGCCCAACTATCTGCCTCTGGAGCTCTGTGTGCTTGTTTCATTACAACGGTATACGAAAGCATTATCTTCACAGCAGAGGGCATCCTTAGTTGAAAAATCAAGGCAGAAGCCTCAGGATAGAATACGAACTGTGACTGATGCTGTATGCAAATACCAGTATGATAGTGATCCTGTGCTTACTACATGTGGTATTTCTATAGAGAAGCAATTAACTCAGTTTGAAGGGCGCGTTCTTGATACTCCGAAGACACTTTTGAACCCCACTCGCATTGACCGCTGGATTGTTGTCAACTTCTCTGCACGCTGTGATACCAGCCATATCTCGAGGGAGCTTATCAACAGTGGGAGGAAAAAGGGCATTCTTATTGAGCGTCCCTATACACTGCTTGAGGAAGAACCACAGTCTAGAAGACAAAGCCCGGTTATAAGAGTTGAAAAGATGTTTGAACAAATTCAAACTAAGCTTCCAAGTCCACCTGACTTCATTCTTTGTGTTTTACCAGAGAGGAAAAATTCTGATATTTATGGGCCTTGGAAGAAAAAGAGTCTCAGTGATTTCGGTATTGTCACACAATGCATTTCCCCTTCCAAGATTAATGATCAGTACCTTACCAATCTTGGAGGAATAAATTCTTTGTTGGCGCTAGAGCATTCCTCACGTTTCCCTTTGATAAAAGATACTCCTACGATGATTTTGGGGATGGATGTATCTCATGGGTCTCCTGGTCGATCAGACATTCCTTCTGTTGCTGCGGTTGTAGGCTCTCGAAGTTGGCCACTTATTTCAAGGTACAGAGCAGCTGTTAGAACACAATCTCCTAAGGTGGAAATGATTGATGCTCTTTACAAGCCACTGGCAAATGGAAAAGATGATGGTATTATCAGGGAACTGCTTGTCGACTTCTATAAGACAAGTGGTGGACGTAAACCAACTCAGATTATTGTCTTCAGGGATGGAGTAAGTGAATCACAGTTTAATCAAGTCCTGAACATTGAGCTGGATCAAATCATAAAGGTATGTCCTGCAATTCAGTGCTTACCAGCACCTAGGGAGGCTAATATTCCAAAGTTTACTGTGATTGTGGCTCAGAAGAATCATCACACAAAGCTATTCCAAACTAATTCCCCTGATAATGTTCCACCAGGGACTGTTGTTGACACCAAAATTGTGCATCCAAGGAATTATGATTTCTACATGTGTGCTCAGGCAGGCATGATTGGAACTTCCAGGCCAGCTCACTATCATGTCTTGCTTGATGAGATTGGCTTTTCCCCTGATGACTTGCAAAACCTGATCCACTCCCTTTCTTATGTGTACCAGAGGAGTACAACTGCAATCTCTATTGTGGCGCCCATATGCTATGCCCACCTTGCTGCAGCACAAGTGGGGCAGTTCATGAAGTTTGAAGATCTCTCCGACACCTCATCGGGGAAGGGCAGTGTCACTTCATCAGAGAGCATCCCAGTTCAAGAGCTTCCCAGGCTTCATGAGAAGGTCCAGGGCTCCATGTTCTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

835

Amino Acids

92.95

Weight (kDa)

9.15

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 53 - 162 8.4e-10 N-terminal domain of argonaute
ArgoL1 PF08699 174 - 221 2.3e-17 Argonaute linker 1 domain
PAZ PF02170 228 - 358 1.7e-29 PAZ domain
ArgoL2 PF16488 368 - 411 4.6e-08 Argonaute linker 2 domain
Piwi PF02171 490 - 796 1.6e-94 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AarI CACCTGC 2 cut(s) 26, 563
Acc36I ACCTGC 3 cut(s) 26, 563, 963
AccB1I GGYRCC 1 cut(s) 2335
AccI GTMKAC 3 cut(s) 444, 1037, 1884
AccII CGCG 1 cut(s) 1215
AciI CCGC 4 cut(s) 80, 721, 1261, 1739
AclWI GGATC 4 cut(s) 49, 1148, 1995, 2275
AcoI YGGCCR 1 cut(s) 1760
AcsI RAATTY 4 cut(s) 794, 1437, 1498, 1615
AcuI CTGAAG 3 cut(s) 341, 428, 1918
AcyI GRCGYC 1 cut(s) 2336
AdeI CACNNNGTG 1 cut(s) 1292
AfaI GTAC 6 cut(s) 142, 522, 1592, 1778, 2303, 2314
AfiI CCNNNNNNNGG 4 cut(s) 620, 790, 866, 2415
AflIII ACRYGT 2 cut(s) 1169, 2181
AgeI ACCGGT 1 cut(s) 676
AjnI CCWGG 9 cut(s) 548, 670, 702, 859, 1708, 2128, 2213, 2465, 2484
AloI GAACNNNNNNTCC 2 cut(s) 1786, 1818
Alw21I GWGCWC 2 cut(s) 1011, 2191
Alw26I GTCTC 5 cut(s) 151, 251, 878, 1538, 1710
AlwI GGATC 4 cut(s) 49, 1148, 1995, 2275
AlwNI CAGNNNCTG 2 cut(s) 212, 2135
Ama87I CYCGRG 1 cut(s) 1309
AoxI GGCC 5 cut(s) 741, 986, 1514, 1760, 2216
ApeKI GCWGC 7 cut(s) 392, 490, 497, 1736, 1784, 2360, 2363
ApoI RAATTY 4 cut(s) 794, 1437, 1498, 1615
AseI ATTAAT 1 cut(s) 1581
AsiGI ACCGGT 1 cut(s) 676
Asp700I GAANNNNTTC 2 cut(s) 1437, 1615
AspA2I CCTAGG 1 cut(s) 2033
AspLEI GCGC 3 cut(s) 1215, 1630, 2338
AspS9I GGNCC 4 cut(s) 674, 987, 1514, 2482
AsuC2I CCSGG 1 cut(s) 1406
AsuHPI GGTGA 2 cut(s) 875, 907
AvaI CYCGRG 1 cut(s) 1309
AvaII GGWCC 2 cut(s) 674, 2482
AvrII CCTAGG 1 cut(s) 2033
AxyI CCTNAGG 2 cut(s) 1098, 1806
BaeI ACNNNNGTAYC 2 cut(s) 512, 545
BalI TGGCCA 1 cut(s) 1762
BanI GGYRCC 1 cut(s) 2335
BanII GRGCYC 2 cut(s) 1011, 2492
BbsI GAAGAC 3 cut(s) 1238, 1402, 1924
Bbv12I GWGCWC 2 cut(s) 1011, 2191
BbvI GCAGC 7 cut(s) 379, 502, 509, 1723, 1796, 2347, 2375
BccI CCATC 4 cut(s) 860, 1681, 1853, 1934
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 9 cut(s) 550, 672, 704, 861, 1710, 2130, 2215, 2467, 2486
BclI TGATCA 2 cut(s) 660, 1585
BcnI CCSGG 1 cut(s) 1406
BcoDI GTCTC 5 cut(s) 151, 251, 878, 1538, 1710
BfaI CTAG 3 cut(s) 1392, 1631, 2034
BfmI CTRYAG 3 cut(s) 888, 1182, 2361
BfoI RGCGCY 2 cut(s) 1631, 2339
BfuAI ACCTGC 3 cut(s) 26, 563, 963
BglII AGATCT 1 cut(s) 2395
BisI GCNGC 7 cut(s) 393, 491, 498, 1737, 1785, 2361, 2364
BlnI CCTAGG 1 cut(s) 2033
BlsI GCNGC 7 cut(s) 394, 492, 499, 1738, 1786, 2362, 2365
Bme18I GGWCC 2 cut(s) 674, 2482
BmeT110I CYCGRG 1 cut(s) 1309
BmgT120I GGNCC 4 cut(s) 674, 987, 1514, 2482
BmiI GGNNCC 3 cut(s) 45, 2337, 2491
BmrI ACTGGG 5 cut(s) 162, 288, 315, 742, 2444
BmsI GCATC 9 cut(s) 7, 199, 448, 819, 1076, 1114, 1813, 2167, 2454
BmuI ACTGGG 5 cut(s) 162, 288, 315, 742, 2444
BpiI GAAGAC 3 cut(s) 1238, 1402, 1924
BplI GAGNNNNNCTC 2 cut(s) 1625, 1657
BpmI CTGGAG 3 cut(s) 189, 532, 1025
Bpu10I CCTNAGC 1 cut(s) 2190
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 1 cut(s) 1406
BsaBI GATNNNNATC 1 cut(s) 1152
BsaHI GRCGYC 1 cut(s) 2336
BsaI GGTCTC 1 cut(s) 1710
BsaJI CCNNGG 7 cut(s) 703, 1517, 2033, 2129, 2162, 2465, 2485
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 8 cut(s) 307, 337, 426, 456, 530, 560, 1786, 1816
Bsc4I CCNNNNNNNGG 4 cut(s) 620, 790, 866, 2415
Bse118I RCCGGY 1 cut(s) 676
Bse21I CCTNAGG 2 cut(s) 1098, 1806
Bse8I GATNNNNATC 1 cut(s) 1152
BseBI CCWGG 9 cut(s) 550, 672, 704, 861, 1710, 2130, 2215, 2467, 2486
BseDI CCNNGG 7 cut(s) 703, 1517, 2033, 2129, 2162, 2465, 2485
BseGI GGATG 9 cut(s) 13, 463, 649, 1067, 1692, 1696, 1945, 2158, 2445
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 4 cut(s) 620, 790, 866, 2415
BseMII CTCAG 9 cut(s) 95, 467, 632, 1112, 1214, 1549, 1934, 2087, 2204
BseRI GAGGAG 2 cut(s) 57, 2323
BseXI GCAGC 7 cut(s) 379, 502, 509, 1723, 1796, 2347, 2375
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 1269
Bsh1236I CGCG 1 cut(s) 1215
BshFI GGCC 5 cut(s) 743, 988, 1516, 1762, 2218
BshNI GGYRCC 1 cut(s) 2335
BshTI ACCGGT 1 cut(s) 676
BsiHKAI GWGCWC 2 cut(s) 1011, 2191
BsiHKCI CYCGRG 1 cut(s) 1309
BsiSI CCGG 2 cut(s) 677, 1406
BslFI GGGAC 3 cut(s) 303, 1344, 2146
BslI CCNNNNNNNGG 4 cut(s) 620, 790, 866, 2415
BsmAI GTCTC 5 cut(s) 151, 251, 878, 1538, 1710
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 3 cut(s) 303, 1344, 2146
BsmI GAATGC 3 cut(s) 756, 1344, 1636
BsnI GGCC 5 cut(s) 743, 988, 1516, 1762, 2218
Bso31I GGTCTC 1 cut(s) 1710
BsoBI CYCGRG 1 cut(s) 1309
Bsp1286I GDGCHC 3 cut(s) 1011, 2191, 2492
Bsp143I GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
BspACI CCGC 4 cut(s) 80, 721, 1261, 1739
BspANI GGCC 5 cut(s) 743, 988, 1516, 1762, 2218
BspCNI CTCAG 9 cut(s) 96, 466, 631, 1111, 1213, 1548, 1933, 2086, 2203
BspFNI CGCG 1 cut(s) 1215
BspHI TCATGA 3 cut(s) 663, 2382, 2473
BspLI GGNNCC 3 cut(s) 45, 2337, 2491
BspMAI CTGCAG 2 cut(s) 892, 2365
BspMI ACCTGC 3 cut(s) 26, 563, 963
BspPI GGATC 4 cut(s) 49, 1148, 1995, 2275
BspT107I GGYRCC 1 cut(s) 2335
BspTNI GGTCTC 1 cut(s) 1710
BsrFI RCCGGY 1 cut(s) 676
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 7 cut(s) 703, 1517, 2033, 2129, 2162, 2465, 2485
BssMI GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
BssNAI GTATAC 1 cut(s) 1038
BssNI GRCGYC 1 cut(s) 2336
BssT1I CCWWGG 3 cut(s) 1517, 2033, 2162
Bst1107I GTATAC 1 cut(s) 1038
Bst2UI CCWGG 9 cut(s) 550, 672, 704, 861, 1710, 2130, 2215, 2467, 2486
Bst6I CTCTTC 2 cut(s) 296, 360
BstACI GRCGYC 1 cut(s) 2336
BstC8I GCNNGC 5 cut(s) 174, 495, 1288, 2197, 2220
BstF5I GGATG 9 cut(s) 13, 463, 649, 1067, 1692, 1696, 1945, 2158, 2445
BstFNI CGCG 1 cut(s) 1215
BstH2I RGCGCY 2 cut(s) 1631, 2339
BstHHI GCGC 3 cut(s) 1215, 1630, 2338
BstKTI GATC 9 cut(s) 57, 353, 663, 1156, 1588, 1718, 1990, 2283, 2398
BstMAI GTCTC 5 cut(s) 151, 251, 878, 1538, 1710
BstMBI GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
BstMWI GCNNNNNNNGC 7 cut(s) 32, 209, 1006, 1015, 1260, 2357, 2467
BstNI CCWGG 9 cut(s) 550, 672, 704, 861, 1710, 2130, 2215, 2467, 2486
BstNSI RCATGY 2 cut(s) 1173, 2185
BstSFI CTRYAG 3 cut(s) 888, 1182, 2361
BstUI CGCG 1 cut(s) 1215
BstV1I GCAGC 7 cut(s) 379, 502, 509, 1723, 1796, 2347, 2375
BstV2I GAAGAC 3 cut(s) 1238, 1402, 1924
BstX2I RGATCY 1 cut(s) 2395
BstXI CCANNNNNNTGG 1 cut(s) 328
BstYI RGATCY 1 cut(s) 2395
BstZ17I GTATAC 1 cut(s) 1038
Bsu36I CCTNAGG 2 cut(s) 1098, 1806
BsuRI GGCC 5 cut(s) 743, 988, 1516, 1762, 2218
BtsCI GGATG 9 cut(s) 13, 463, 649, 1067, 1692, 1696, 1945, 2158, 2445
BtsI GCAGTG 2 cut(s) 1367, 2431
BtsIMutI CAGTG 9 cut(s) 192, 328, 922, 1333, 1367, 1543, 1838, 2024, 2431
BveI ACCTGC 3 cut(s) 26, 563, 963
Cac8I GCNNGC 5 cut(s) 174, 495, 1288, 2197, 2220
CaiI CAGNNNCTG 2 cut(s) 212, 2135
CciI TCATGA 3 cut(s) 663, 2382, 2473
CfoI GCGC 3 cut(s) 1215, 1630, 2338
Cfr10I RCCGGY 1 cut(s) 676
Cfr13I GGNCC 4 cut(s) 674, 987, 1514, 2482
CseI GACGC 2 cut(s) 168, 1346
Csp6I GTAC 6 cut(s) 141, 521, 1591, 1777, 2302, 2313
CspAI ACCGGT 1 cut(s) 676
CspCI CAANNNNNGTGG 4 cut(s) 907, 942, 1237, 1272
CviQI GTAC 6 cut(s) 141, 521, 1591, 1777, 2302, 2313
DinI GGCGCC 1 cut(s) 2337
DpnI GATC 9 cut(s) 56, 352, 662, 1155, 1587, 1717, 1989, 2282, 2397
DpnII GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
DraI TTTAAA 1 cut(s) 799
DraIII CACNNNGTG 1 cut(s) 1292
EaeI YGGCCR 1 cut(s) 1760
Eam1104I CTCTTC 2 cut(s) 296, 360
EarI CTCTTC 2 cut(s) 296, 360
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 1009
Eco130I CCWWGG 3 cut(s) 1517, 2033, 2162
Eco24I GRGCYC 2 cut(s) 1011, 2492
Eco31I GGTCTC 1 cut(s) 1710
Eco47I GGWCC 2 cut(s) 674, 2482
Eco53kI GAGCTC 1 cut(s) 1009
Eco57I CTGAAG 3 cut(s) 341, 428, 1918
Eco81I CCTNAGG 2 cut(s) 1098, 1806
Eco88I CYCGRG 1 cut(s) 1309
EcoICRI GAGCTC 1 cut(s) 1009
EcoRII CCWGG 9 cut(s) 548, 670, 702, 859, 1708, 2128, 2213, 2465, 2484
EcoT14I CCWWGG 3 cut(s) 1517, 2033, 2162
EcoT22I ATGCAT 2 cut(s) 463, 1565
EcoT38I GRGCYC 2 cut(s) 1011, 2492
EgeI GGCGCC 1 cut(s) 2337
EheI GGCGCC 1 cut(s) 2337
ErhI CCWWGG 3 cut(s) 1517, 2033, 2162
Esp3I CGTCTC 1 cut(s) 151
FaqI GGGAC 3 cut(s) 303, 1344, 2146
FauI CCCGC 1 cut(s) 728
FauNDI CATATG 1 cut(s) 2342
FbaI TGATCA 2 cut(s) 660, 1585
FblI GTMKAC 3 cut(s) 444, 1037, 1884
Fnu4HI GCNGC 7 cut(s) 393, 491, 498, 1737, 1785, 2361, 2364
FokI GGATG 9 cut(s) 20, 470, 656, 1054, 1699, 1703, 1952, 2145, 2432
FriOI GRGCYC 2 cut(s) 1011, 2492
Fsp4HI GCNGC 7 cut(s) 393, 491, 498, 1737, 1785, 2361, 2364
FspBI CTAG 3 cut(s) 1392, 1631, 2034
GlaI GCGC 3 cut(s) 1214, 1629, 2337
GluI GCNGC 7 cut(s) 393, 491, 498, 1737, 1785, 2361, 2364
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 3 cut(s) 189, 532, 1025
HaeII RGCGCY 2 cut(s) 1631, 2339
HaeIII GGCC 5 cut(s) 743, 988, 1516, 1762, 2218
HapII CCGG 2 cut(s) 677, 1406
HgaI GACGC 2 cut(s) 168, 1346
HhaI GCGC 3 cut(s) 1215, 1630, 2338
Hin1I GRCGYC 1 cut(s) 2336
Hin6I GCGC 3 cut(s) 1213, 1628, 2336
HinP1I GCGC 3 cut(s) 1213, 1628, 2336
HincII GTYRAC 4 cut(s) 445, 1276, 1885, 2143
HindII GTYRAC 4 cut(s) 445, 1276, 1885, 2143
HindIII AAGCTT 1 cut(s) 1448
HinfI GANTC 7 cut(s) 259, 316, 545, 667, 1531, 1952, 2080
HpaII CCGG 2 cut(s) 677, 1406
HphI GGTGA 2 cut(s) 875, 907
Hpy188I TCNGA 9 cut(s) 408, 693, 1230, 1504, 1720, 1923, 2076, 2404, 2440
HpyAV CCTTC 8 cut(s) 25, 524, 872, 1202, 1581, 1737, 2413, 2473
HpyCH4IV ACGT 2 cut(s) 1645, 1909
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 209, 1006, 1015, 1260, 2357, 2467
HpySE526I ACGT 2 cut(s) 1645, 1909
Hsp92I GRCGYC 1 cut(s) 2336
HspAI GCGC 3 cut(s) 1213, 1628, 2336
KasI GGCGCC 1 cut(s) 2335
Ksp22I TGATCA 2 cut(s) 660, 1585
Kzo9I GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
LmnI GCTCC 5 cut(s) 208, 434, 1006, 1315, 2495
Lsp1109I GCAGC 7 cut(s) 379, 502, 509, 1723, 1796, 2347, 2375
LweI GCATC 9 cut(s) 7, 199, 448, 819, 1076, 1114, 1813, 2167, 2454
MaeI CTAG 3 cut(s) 1392, 1631, 2034
MaeII ACGT 2 cut(s) 1645, 1909
MaeIII GTNAC 6 cut(s) 190, 785, 895, 1117, 1552, 2428
MalI GATC 9 cut(s) 56, 352, 662, 1155, 1587, 1717, 1989, 2282, 2397
MboI GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
MfeI CAATTG 2 cut(s) 167, 414
MflI RGATCY 1 cut(s) 2395
MhlI GDGCHC 3 cut(s) 1011, 2191, 2492
MlsI TGGCCA 1 cut(s) 1762
MluNI TGGCCA 1 cut(s) 1762
Mly113I GGCGCC 1 cut(s) 2336
MlyI GAGTC 4 cut(s) 325, 539, 661, 1540
MmeI TCCRAC 3 cut(s) 95, 638, 2427
Mox20I TGGCCA 1 cut(s) 1762
Mph1103I ATGCAT 2 cut(s) 463, 1565
MroXI GAANNNNTTC 2 cut(s) 1437, 1615
MscI TGGCCA 1 cut(s) 1762
MseI TTAA 7 cut(s) 383, 771, 798, 854, 1196, 1581, 1962
MslI CAYNNNNRTG 1 cut(s) 2229
Msp20I TGGCCA 1 cut(s) 1762
MspA1I CMGCKG 2 cut(s) 1263, 1787
MspI CCGG 2 cut(s) 677, 1406
MunI CAATTG 2 cut(s) 167, 414
Mva1269I GAATGC 3 cut(s) 756, 1344, 1636
MvaI CCWGG 9 cut(s) 550, 672, 704, 861, 1710, 2130, 2215, 2467, 2486
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 7 cut(s) 32, 209, 1006, 1015, 1260, 2357, 2467
NarI GGCGCC 1 cut(s) 2336
NciI CCSGG 1 cut(s) 1406
NdeI CATATG 1 cut(s) 2342
NdeII GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
NlaIV GGNNCC 3 cut(s) 45, 2337, 2491
NmuCI GTSAC 4 cut(s) 190, 1117, 1552, 2428
NsiI ATGCAT 2 cut(s) 463, 1565
NspI RCATGY 2 cut(s) 1173, 2185
PaeR7I CTCGAG 1 cut(s) 1309
PagI TCATGA 3 cut(s) 663, 2382, 2473
PaqCI CACCTGC 2 cut(s) 26, 563
PciI ACATGT 2 cut(s) 1169, 2181
PcsI WCGNNNNNNNCGW 1 cut(s) 346
PctI GAATGC 3 cut(s) 756, 1344, 1636
PdmI GAANNNNTTC 2 cut(s) 1437, 1615
PfeI GAWTC 3 cut(s) 259, 1952, 2080
PfoI TCCNGGA 2 cut(s) 548, 670
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 7 cut(s) 394, 492, 499, 1738, 1786, 2362, 2365
PleI GAGTC 4 cut(s) 324, 539, 661, 1539
PluTI GGCGCC 1 cut(s) 2339
PpsI GAGTC 4 cut(s) 324, 539, 661, 1539
PscI ACATGT 2 cut(s) 1169, 2181
PshBI ATTAAT 1 cut(s) 1581
PsiI TTATAA 1 cut(s) 1412
Psp124BI GAGCTC 1 cut(s) 1011
Psp6I CCWGG 9 cut(s) 548, 670, 702, 859, 1708, 2128, 2213, 2465, 2484
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 9 cut(s) 548, 670, 702, 859, 1708, 2128, 2213, 2465, 2484
PspN4I GGNNCC 3 cut(s) 45, 2337, 2491
PspPI GGNCC 4 cut(s) 674, 987, 1514, 2482
PstI CTGCAG 2 cut(s) 892, 2365
PstNI CAGNNNCTG 2 cut(s) 212, 2135
PsuI RGATCY 1 cut(s) 2395
PvuII CAGCTG 1 cut(s) 1787
RsaI GTAC 6 cut(s) 142, 522, 1592, 1778, 2303, 2314
RsaNI GTAC 6 cut(s) 141, 521, 1591, 1777, 2302, 2313
RseI CAYNNNNRTG 1 cut(s) 2229
SacI GAGCTC 1 cut(s) 1011
SalI GTCGAC 2 cut(s) 443, 1883
SaqAI TTAA 7 cut(s) 383, 771, 798, 854, 1196, 1581, 1962
SatI GCNGC 7 cut(s) 393, 491, 498, 1737, 1785, 2361, 2364
Sau3AI GATC 9 cut(s) 54, 350, 660, 1153, 1585, 1715, 1987, 2280, 2395
Sau96I GGNCC 4 cut(s) 674, 987, 1514, 2482
SchI GAGTC 4 cut(s) 325, 539, 661, 1540
SduI GDGCHC 3 cut(s) 1011, 2191, 2492
SfaNI GCATC 9 cut(s) 7, 199, 448, 819, 1076, 1114, 1813, 2167, 2454
SfcI CTRYAG 3 cut(s) 888, 1182, 2361
SfoI GGCGCC 1 cut(s) 2337
Sfr274I CTCGAG 1 cut(s) 1309
SinI GGWCC 2 cut(s) 674, 2482
SlaI CTCGAG 1 cut(s) 1309
SmiMI CAYNNNNRTG 1 cut(s) 2229
SmlI CTYRAG 2 cut(s) 1309, 1373
SmoI CTYRAG 2 cut(s) 1309, 1373
SsiI CCGC 4 cut(s) 80, 721, 1261, 1739
SspDI GGCGCC 1 cut(s) 2335
SspI AATATT 1 cut(s) 2047
SspMI CTAG 3 cut(s) 1392, 1631, 2034
SstI GAGCTC 1 cut(s) 1011
StyI CCWWGG 3 cut(s) 1517, 2033, 2162
TaiI ACGT 2 cut(s) 1648, 1912
TaqI TCGA 5 cut(s) 444, 1310, 1714, 1753, 1884
TatI WGTACW 1 cut(s) 2312
TfiI GAWTC 3 cut(s) 259, 1952, 2080
Tru1I TTAA 7 cut(s) 383, 771, 798, 854, 1196, 1581, 1962
Tru9I TTAA 7 cut(s) 383, 771, 798, 854, 1196, 1581, 1962
TscAI CASTG 9 cut(s) 199, 328, 929, 1333, 1374, 1543, 1845, 2024, 2431
TseFI GTSAC 4 cut(s) 190, 1117, 1552, 2428
TseI GCWGC 7 cut(s) 392, 490, 497, 1736, 1784, 2360, 2363
Tsp45I GTSAC 4 cut(s) 190, 1117, 1552, 2428
TspDTI ATGAA 8 cut(s) 587, 863, 1014, 1459, 2371, 2399, 2424, 2462
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 9 cut(s) 199, 328, 929, 1333, 1374, 1543, 1845, 2024, 2431
VpaK11BI GGWCC 2 cut(s) 674, 2482
VspI ATTAAT 1 cut(s) 1581
XapI RAATTY 4 cut(s) 794, 1437, 1498, 1615
XbaI TCTAGA 1 cut(s) 1391
XceI RCATGY 2 cut(s) 1173, 2185
XhoI CTCGAG 1 cut(s) 1309
XmaJI CCTAGG 1 cut(s) 2033
XmiI GTMKAC 3 cut(s) 444, 1037, 1884
XmnI GAANNNNTTC 2 cut(s) 1437, 1615
XspI CTAG 3 cut(s) 1392, 1631, 2034
Zsp2I ATGCAT 2 cut(s) 463, 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.