Rw2G043870

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001253
Physical Location & Seq
Reverse (-)
70402506 .. 70408401
5896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043870.1

Sequence Viewer

Length: 2739 bp
ATGGTAGCTGGTGCCAAAAATGCAGGTGGGGACGCAGATTTGCCCCCACCCCCTCCAACCATACCATCCAATTTGAAGCCAGAACTATTGGCTCCGCCCAAGTATTCTATTATGACTAGGCCATGCAGGACTGGAACTACTGGACGGGGCATTACTTTGCTTGCCAACCACTTCAAAGTTTCAGTGAATAATCCAGATGCTGTATTTTATCAGTATTCTGTTCGCATGACTTATGAAGATAATAAGCCAGTTCTTCCAGGGATGGGAATTGGGAGAAAATTGATGGATAGGCTTTATCATACATACTCCATGGAATTTGATGGCAAAAAGTTTGCTTACGATGGGGAGAAATCTCTGTACACATTTGGTCCTCTACCACTGCACAAGCTCAATTACACAGTTGTGCTGGAGGAATCAGTTTCTAGGCCACATGAAACCGGCAGCTCTAGTGTCACTGAAAAACGGATGAAACGATCTATTCAGTCAAAGACTTTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAGCCAATTGCTCTTGCCCTTAAAGGAGCTGATGCAGATGATGGTCAAGATGCATTGAGAGTGCTCGACATAGTCTTAAGGCAGCAAGCAGCTAACAGGGGATGTCTTCTAGTAAGACAGTCATTCTTTCACGATGACTCCAGATGCATCACTGATGTTAGAGGTACCGGTTTAAGTACTGTTCGGGGCTTCCATTCCAGTTTTCGTCCCACTCAGGGTGGTCTGACCTTGAATATGGATGTATCTACGACACTGATCTTAACCCCTGGACCAGTGATTGATTTTCTGAAAGATAACCAGGGTGTACAACACCCCCGCAACATTGACTGGGCAAGGGCTAAGAGAATGCTGAAAAATTTGAGGATAAAGACAAGACATCACAACATGGAATTCAAAATTACTGGCTTGAGCCAGAATCCCTGCCGTGAGCAATATTTTCCTATGAAATTGAGAAGTGATGACGGAACTACTGTGGAGCAGACTGTGGACATTACTGTATATGATTATTTCACCAAACATCGTGGCATAGAACTTACCTATTCTGCATACTTTCCATGCCTTGATGTTGGCAAGCCAAAAAGGCCTAACTATGTGCCACTCGAGCTTTGTTCCCTTGTTTCCCTCCAACGGTATAAAAAAGCATTATCTTCAAAGCAGAGAGCTTCTATAGTTGGAAGTTCAAGGCAGAGGCCTCAGGAGAGAATAAACAGTGTGACTAATGCTGTAAGGAATTATGGCTATAATGATGATCCCGTGCTTGCTGCATGCGGCATATCCATAGAGAAGCAATTGACTCAGATTGAAGGTCGTGTACTTGAGGCTCCGAAGTTGAAGGTAGGCAACAGTTCCGATTGCATGCCTTCAGATGGGAGATGGAACTTCAATGGCAAGACATTTTTGGACCCCAAACACATTGACAAATGGATTGTTGTCAACTTCTCTTTGGAAAATTGTGATACTAGTCGCATTTCAAGGCAGCTTTGTGACATTGGGAGGAAGAAGGGCATTAGAATTGAGTGCCCAATCACATTGATTGAGGAACAACCACAGCTTAAAAAACAAAGCCCTATTGAAAGAGTTAAAAAGATGTTTGAACAGATTAAAGTGAAGCATCCTAAAGAGCCACCAAACTTCATTCTTTGCATTTTACCAGAAAGAAAAACTTCTGATATATATGGACCGTGGAAGAAAGTGTGCCTTGTTGACTATGGCATTGTCACACAGTGCATTTCTCCTCCCATGAAAAAAAATACTAGGGAGAGAGAGCTATCTGAACAGTACCTCACTAATGTACTTTTGAAAATCAATTCTAAGCTTGGAGGAATAAATTCTTTGTTGGCAACAGAACACTCTGGGGTTGTTTCTCTAATTAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGCCTCCTGGTCGATCAGATATTCCTTCTGTTGCTGCGGTTGTTGGCTCTCTACAGTGGCCACTAATTTCACAGTACAGAGCGGCTGTAAGGACACAAAAAGTTCGTAAGGAGATGATTGATTCTCTGTACAAGCCACTGGAAAGTGGAGAAGATGATGGTATTATGAGGGAACTGCTTAAGGAATTCTATGAAACTAGCAAATGCAAACCAACTCAGATTATTATCTTCAGGGATGGAGTCAGCGAATCGCAATTTAATCAAGTTTTGAATGAGGAGCTGGATCAAATTATGAAGGCTTACAAGCACCACCAGAAGGTTGATGAGCTTCCAAAATTTACTGTGATTGTGGCTCAAAAGAACCATAATACAAAGCTATTCCAATCTGGTTCTCCCCAGTATAATGTTCCACCAGGAACAGTTGTGGACACAAATATTGTGCATCCAAGGAATTATGATTTCTATATATGTGCTCACAAAGGCTCCATTGGAACTTCTAGGCCAGTACACTATCATGTCTTGCTCGATGAGATTGGCTTTTCTGCCAATGAACTGCAAAACCTGATCCACTCTCTTTCCTATGTGTACCAGAGGAGTACAACTGCAGTCTCAATTGTTGCGCCCATATGTTATGCGCACCTTGCTGCAGCCCAAGTAATGCAGTTTATGAAGTTTGAAGATCTCTCGGCAACTTCATCATCAGGAAGGGTCAGTGTCACTACATCAGAGAGTATCCCAGTGCCTGAGCTCCCTAGGCTTCATGAGAAGGTCCGCGGTTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

912

Amino Acids

102.56

Weight (kDa)

9.24

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 50 - 203 1.3e-25 N-terminal domain of argonaute
ArgoL1 PF08699 215 - 263 1.7e-14 Argonaute linker 1 domain
PAZ PF02170 270 - 400 3.8e-31 PAZ domain
ArgoL2 PF16488 410 - 455 1.5e-14 Argonaute linker 2 domain
ArgoMid PF16487 463 - 517 1.4e-06 Mid domain of argonaute
Piwi PF02171 555 - 872 1.6e-104 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc16I TGCGCA 1 cut(s) 2580
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 2 cut(s) 11, 695
AccBSI CCGCTC 1 cut(s) 2027
AccII CGCG 1 cut(s) 2718
AciI CCGC 7 cut(s) 95, 847, 1299, 1982, 2027, 2716, 2718
AclWI GGATC 3 cut(s) 1274, 2235, 2503
AcoI YGGCCR 1 cut(s) 2003
AcsI RAATTY 6 cut(s) 314, 886, 920, 1858, 2129, 2279
AcuI CTGAAG 3 cut(s) 551, 1377, 2158
AdeI CACNNNGTG 1 cut(s) 1755
AfiI CCNNNNNNNGG 5 cut(s) 263, 746, 1158, 1397, 2260
AflII CTTAAG 2 cut(s) 607, 2123
AgeI ACCGGT 1 cut(s) 698
AhlI ACTAGT 1 cut(s) 1490
AjnI CCWGG 5 cut(s) 256, 796, 828, 1951, 2356
AleI CACNNNNGTG 1 cut(s) 401
Alw21I GWGCWC 3 cut(s) 597, 2419, 2694
Alw26I GTCTC 1 cut(s) 2557
AlwI GGATC 3 cut(s) 1274, 2235, 2503
AlwNI CAGNNNCTG 2 cut(s) 200, 2687
Ama87I CYCGRG 1 cut(s) 1130
AoxI GGCC 7 cut(s) 119, 425, 1112, 1220, 1946, 2003, 2444
ApeKI GCWGC 9 cut(s) 441, 515, 613, 620, 1292, 1507, 1979, 2588, 2591
ApoI RAATTY 6 cut(s) 314, 886, 920, 1858, 2129, 2279
AsiGI ACCGGT 1 cut(s) 698
Asp700I GAANNNNTTC 2 cut(s) 1693, 1858
Asp718I GGTACC 1 cut(s) 695
AspA2I CCTAGG 1 cut(s) 2696
AspLEI GCGC 2 cut(s) 2566, 2581
AspS9I GGNCC 6 cut(s) 368, 800, 1432, 1709, 1946, 2713
AsuHPI GGTGA 1 cut(s) 1033
AvaI CYCGRG 1 cut(s) 1130
AvaII GGWCC 5 cut(s) 368, 800, 1432, 1709, 2713
AvrII CCTAGG 1 cut(s) 2696
AxyI CCTNAGG 1 cut(s) 1224
BaeGI GKGCMC 1 cut(s) 1553
BalI TGGCCA 1 cut(s) 2005
BanI GGYRCC 2 cut(s) 11, 695
BanII GRGCYC 1 cut(s) 2694
BarI GAAGNNNNNNTAC 2 cut(s) 1326, 1358
BbsI GAAGAC 1 cut(s) 629
Bbv12I GWGCWC 3 cut(s) 597, 2419, 2694
BbvI GCAGC 9 cut(s) 453, 502, 625, 632, 1279, 1519, 1966, 2575, 2603
BceAI ACGGC 1 cut(s) 939
BciT130I CCWGG 5 cut(s) 258, 798, 830, 1953, 2358
BciVI GTATCC 1 cut(s) 2687
BcoDI GTCTC 1 cut(s) 2557
BcuI ACTAGT 1 cut(s) 1490
BfaI CTAG 9 cut(s) 117, 423, 447, 641, 1491, 1785, 2142, 2442, 2697
BfmI CTRYAG 4 cut(s) 1197, 1997, 2547, 2589
BfrI CTTAAG 2 cut(s) 607, 2123
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 2687
BglI GCCNNNNNGGC 1 cut(s) 1111
BglII AGATCT 1 cut(s) 2623
BlnI CCTAGG 1 cut(s) 2696
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 5 cut(s) 258, 798, 830, 1953, 2358
Bme18I GGWCC 5 cut(s) 368, 800, 1432, 1709, 2713
BmeT110I CYCGRG 1 cut(s) 1130
BmgT120I GGNCC 6 cut(s) 368, 800, 1432, 1709, 1946, 2713
BmiI GGNNCC 7 cut(s) 13, 93, 697, 1353, 1434, 2428, 2722
BmrFI CCNGG 5 cut(s) 258, 798, 830, 1953, 2358
BmrI ACTGGG 3 cut(s) 868, 2335, 2675
BmsI GCATC 7 cut(s) 187, 553, 571, 665, 687, 1651, 2395
BmuI ACTGGG 3 cut(s) 868, 2335, 2675
BpiI GAAGAC 1 cut(s) 629
BpmI CTGGAG 2 cut(s) 428, 655
Bpu10I CCTNAGC 1 cut(s) 2688
BpuEI CTTGAG 2 cut(s) 958, 1367
BsaBI GATNNNNATC 1 cut(s) 2168
BsaJI CCNNGG 8 cut(s) 257, 309, 796, 829, 1712, 2390, 2696, 2716
BsaWI WCCGGW 1 cut(s) 698
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 2411, 2441
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 746, 1158, 1397, 2260
Bse118I RCCGGY 2 cut(s) 437, 698
Bse21I CCTNAGG 1 cut(s) 1224
Bse8I GATNNNNATC 1 cut(s) 2168
BseBI CCWGG 5 cut(s) 258, 798, 830, 1953, 2358
BseDI CCNNGG 8 cut(s) 257, 309, 796, 829, 1712, 2390, 2696, 2716
BseJI GATNNNNATC 1 cut(s) 2168
BseLI CCNNNNNNNGG 5 cut(s) 263, 746, 1158, 1397, 2260
BseMII CTCAG 5 cut(s) 758, 1238, 1340, 2174, 2679
BseRI GAGGAG 3 cut(s) 1755, 2234, 2551
BseSI GKGCMC 1 cut(s) 1553
BseXI GCAGC 9 cut(s) 453, 502, 625, 632, 1279, 1519, 1966, 2575, 2603
BsgI GTGCAG 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 2718
BshFI GGCC 7 cut(s) 121, 427, 1114, 1222, 1948, 2005, 2446
BshNI GGYRCC 2 cut(s) 11, 695
BshTI ACCGGT 1 cut(s) 698
BsiHKAI GWGCWC 3 cut(s) 597, 2419, 2694
BsiHKCI CYCGRG 1 cut(s) 1130
BsiSI CCGG 2 cut(s) 438, 699
BslFI GGGAC 2 cut(s) 44, 723
BslI CCNNNNNNNGG 5 cut(s) 263, 746, 1158, 1397, 2260
BsmAI GTCTC 1 cut(s) 2557
BsmFI GGGAC 2 cut(s) 44, 723
BsmI GAATGC 1 cut(s) 882
BsnI GGCC 7 cut(s) 121, 427, 1114, 1222, 1948, 2005, 2446
BsoBI CYCGRG 1 cut(s) 1130
Bsp1286I GDGCHC 4 cut(s) 597, 1553, 2419, 2694
Bsp1407I TGTACA 3 cut(s) 357, 835, 2073
Bsp143I GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 7 cut(s) 95, 847, 1299, 1982, 2027, 2716, 2718
BspANI GGCC 7 cut(s) 121, 427, 1114, 1222, 1948, 2005, 2446
BspCNI CTCAG 5 cut(s) 757, 1237, 1339, 2173, 2680
BspFNI CGCG 1 cut(s) 2718
BspHI TCATGA 1 cut(s) 2704
BspLI GGNNCC 7 cut(s) 13, 93, 697, 1353, 1434, 2428, 2722
BspMAI CTGCAG 2 cut(s) 2551, 2593
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 3 cut(s) 1274, 2235, 2503
BspT107I GGYRCC 2 cut(s) 11, 695
BspTI CTTAAG 2 cut(s) 607, 2123
BsrBI CCGCTC 1 cut(s) 2027
BsrFI RCCGGY 2 cut(s) 437, 698
BsrGI TGTACA 3 cut(s) 357, 835, 2073
BssAI RCCGGY 2 cut(s) 437, 698
BssECI CCNNGG 8 cut(s) 257, 309, 796, 829, 1712, 2390, 2696, 2716
BssMI GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
BssT1I CCWWGG 3 cut(s) 309, 2390, 2696
Bst2UI CCWGG 5 cut(s) 258, 798, 830, 1953, 2358
BstAFI CTTAAG 2 cut(s) 607, 2123
BstAUI TGTACA 3 cut(s) 357, 835, 2073
BstC8I GCNNGC 6 cut(s) 162, 618, 1103, 1290, 1297, 1388
BstDEI CTNAG 7 cut(s) 744, 870, 1224, 1326, 1842, 2160, 2688
BstDSI CCRYGG 3 cut(s) 309, 1712, 2716
BstFNI CGCG 1 cut(s) 2718
BstHHI GCGC 2 cut(s) 2566, 2581
BstKTI GATC 7 cut(s) 476, 789, 1282, 1961, 2230, 2511, 2626
BstMAI GTCTC 1 cut(s) 2557
BstMBI GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
BstMWI GCNNNNNNNGC 5 cut(s) 20, 1111, 1132, 2585, 2698
BstNI CCWGG 5 cut(s) 258, 798, 830, 1953, 2358
BstNSI RCATGY 2 cut(s) 1299, 1390
BstSCI CCNGG 5 cut(s) 256, 796, 828, 1951, 2356
BstSFI CTRYAG 4 cut(s) 1197, 1997, 2547, 2589
BstSLI GKGCMC 1 cut(s) 1553
BstUI CGCG 1 cut(s) 2718
BstV1I GCAGC 9 cut(s) 453, 502, 625, 632, 1279, 1519, 1966, 2575, 2603
BstV2I GAAGAC 1 cut(s) 629
BstX2I RGATCY 1 cut(s) 2623
BstYI RGATCY 1 cut(s) 2623
Bsu36I CCTNAGG 1 cut(s) 1224
BsuI GTATCC 1 cut(s) 2687
BsuRI GGCC 7 cut(s) 121, 427, 1114, 1222, 1948, 2005, 2446
BtgI CCRYGG 3 cut(s) 309, 1712, 2716
BtsI GCAGTG 1 cut(s) 377
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 6 cut(s) 162, 618, 1103, 1290, 1297, 1388
CaiI CAGNNNCTG 2 cut(s) 200, 2687
CciI TCATGA 1 cut(s) 2704
CfoI GCGC 2 cut(s) 2566, 2581
Cfr10I RCCGGY 2 cut(s) 437, 698
Cfr13I GGNCC 6 cut(s) 368, 800, 1432, 1709, 1946, 2713
Cfr42I CCGCGG 1 cut(s) 2719
CseI GACGC 1 cut(s) 41
CspAI ACCGGT 1 cut(s) 698
CspCI CAANNNNNGTGG 2 cut(s) 1033, 1068
DdeI CTNAG 7 cut(s) 744, 870, 1224, 1326, 1842, 2160, 2688
DpnI GATC 7 cut(s) 475, 788, 1281, 1960, 2229, 2510, 2625
DpnII GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
DraIII CACNNNGTG 1 cut(s) 1755
EaeI YGGCCR 1 cut(s) 2003
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 2692
Eco130I CCWWGG 3 cut(s) 309, 2390, 2696
Eco147I AGGCCT 2 cut(s) 1114, 1222
Eco24I GRGCYC 1 cut(s) 2694
Eco47I GGWCC 5 cut(s) 368, 800, 1432, 1709, 2713
Eco53kI GAGCTC 1 cut(s) 2692
Eco57I CTGAAG 3 cut(s) 551, 1377, 2158
Eco81I CCTNAGG 1 cut(s) 1224
Eco88I CYCGRG 1 cut(s) 1130
EcoICRI GAGCTC 1 cut(s) 2692
EcoRI GAATTC 2 cut(s) 920, 2129
EcoRII CCWGG 5 cut(s) 256, 796, 828, 1951, 2356
EcoT14I CCWWGG 3 cut(s) 309, 2390, 2696
EcoT22I ATGCAT 2 cut(s) 586, 680
EcoT38I GRGCYC 1 cut(s) 2694
ErhI CCWWGG 3 cut(s) 309, 2390, 2696
FalI AAGNNNNNCTT 4 cut(s) 1678, 1710, 1713, 1745
FaqI GGGAC 2 cut(s) 44, 723
FauI CCCGC 1 cut(s) 854
FauNDI CATATG 1 cut(s) 2570
FriOI GRGCYC 1 cut(s) 2694
FspAI RTGCGCAY 1 cut(s) 2580
FspBI CTAG 9 cut(s) 117, 423, 447, 641, 1491, 1785, 2142, 2442, 2697
FspI TGCGCA 1 cut(s) 2580
GlaI GCGC 2 cut(s) 2565, 2580
GsuI CTGGAG 2 cut(s) 428, 655
HaeIII GGCC 7 cut(s) 121, 427, 1114, 1222, 1948, 2005, 2446
HapII CCGG 2 cut(s) 438, 699
HgaI GACGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 2566, 2581
Hin6I GCGC 2 cut(s) 2564, 2579
HinP1I GCGC 2 cut(s) 2564, 2579
HincII GTYRAC 2 cut(s) 1465, 1735
HindII GTYRAC 2 cut(s) 1465, 1735
HindIII AAGCTT 1 cut(s) 1844
HinfI GANTC 7 cut(s) 413, 668, 946, 1324, 2066, 2184, 2192
HpaII CCGG 2 cut(s) 438, 699
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 9 cut(s) 360, 836, 1018, 1343, 1465, 1735, 2371, 2452, 2530
Hpy188III TCNNGA 7 cut(s) 194, 578, 662, 672, 1226, 2646, 2705
Hpy8I GTNNAC 9 cut(s) 360, 836, 1018, 1343, 1465, 1735, 2371, 2452, 2530
HpyAV CCTTC 9 cut(s) 1328, 1357, 1401, 1525, 1980, 2233, 2254, 2643, 2704
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 1111, 1132, 2585, 2698
HpyF3I CTNAG 7 cut(s) 744, 870, 1224, 1326, 1842, 2160, 2688
HspAI GCGC 2 cut(s) 2564, 2579
KpnI GGTACC 1 cut(s) 699
KspI CCGCGG 1 cut(s) 2719
Kzo9I GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
LmnI GCTCC 7 cut(s) 97, 557, 1006, 1357, 2221, 2432, 2697
Lsp1109I GCAGC 9 cut(s) 453, 502, 625, 632, 1279, 1519, 1966, 2575, 2603
LweI GCATC 7 cut(s) 187, 553, 571, 665, 687, 1651, 2395
MaeI CTAG 9 cut(s) 117, 423, 447, 641, 1491, 1785, 2142, 2442, 2697
MaeIII GTNAC 5 cut(s) 451, 1243, 1514, 1747, 2659
MalI GATC 7 cut(s) 475, 788, 1281, 1960, 2229, 2510, 2625
MbiI CCGCTC 1 cut(s) 2027
MboI GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
MfeI CAATTG 3 cut(s) 537, 1319, 2556
MflI RGATCY 1 cut(s) 2623
MhlI GDGCHC 4 cut(s) 597, 1553, 2419, 2694
MlsI TGGCCA 1 cut(s) 2005
MluNI TGGCCA 1 cut(s) 2005
MlyI GAGTC 3 cut(s) 662, 1318, 2193
MmeI TCCRAC 3 cut(s) 80, 1180, 1183
Mox20I TGGCCA 1 cut(s) 2005
Mph1103I ATGCAT 2 cut(s) 586, 680
MroXI GAANNNNTTC 2 cut(s) 1693, 1858
MscI TGGCCA 1 cut(s) 2005
MslI CAYNNNNRTG 2 cut(s) 401, 2457
Msp20I TGGCCA 1 cut(s) 2005
MspA1I CMGCKG 1 cut(s) 2718
MspCI CTTAAG 2 cut(s) 607, 2123
MspI CCGG 2 cut(s) 438, 699
MspR9I CCNGG 5 cut(s) 258, 798, 830, 1953, 2358
MunI CAATTG 3 cut(s) 537, 1319, 2556
Mva1269I GAATGC 1 cut(s) 882
MvaI CCWGG 5 cut(s) 258, 798, 830, 1953, 2358
MvnI CGCG 1 cut(s) 2718
MwoI GCNNNNNNNGC 5 cut(s) 20, 1111, 1132, 2585, 2698
NcoI CCATGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 2570
NdeII GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
NlaIV GGNNCC 7 cut(s) 13, 93, 697, 1353, 1434, 2428, 2722
NmeAIII GCCGAG 1 cut(s) 2609
NmuCI GTSAC 5 cut(s) 451, 1243, 1514, 1747, 2659
NsbI TGCGCA 1 cut(s) 2580
NsiI ATGCAT 2 cut(s) 586, 680
NspI RCATGY 2 cut(s) 1299, 1390
OliI CACNNNNGTG 1 cut(s) 401
PaeI GCATGC 2 cut(s) 1299, 1390
PaeR7I CTCGAG 1 cut(s) 1130
PagI TCATGA 1 cut(s) 2704
PaqCI CACCTGC 1 cut(s) 14
PceI AGGCCT 2 cut(s) 1114, 1222
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PctI GAATGC 1 cut(s) 882
PdmI GAANNNNTTC 2 cut(s) 1693, 1858
PfeI GAWTC 4 cut(s) 413, 946, 2066, 2192
PflFI GACNNNGTC 1 cut(s) 602
PinAI ACCGGT 1 cut(s) 698
PleI GAGTC 3 cut(s) 662, 1318, 2192
PpsI GAGTC 3 cut(s) 662, 1318, 2192
Psp124BI GAGCTC 1 cut(s) 2694
Psp6I CCWGG 5 cut(s) 256, 796, 828, 1951, 2356
PspGI CCWGG 5 cut(s) 256, 796, 828, 1951, 2356
PspN4I GGNNCC 7 cut(s) 13, 93, 697, 1353, 1434, 2428, 2722
PspPI GGNCC 6 cut(s) 368, 800, 1432, 1709, 1946, 2713
PspXI VCTCGAGB 1 cut(s) 1130
PstI CTGCAG 2 cut(s) 2551, 2593
PstNI CAGNNNCTG 2 cut(s) 200, 2687
PsuI RGATCY 1 cut(s) 2623
PsyI GACNNNGTC 1 cut(s) 602
RseI CAYNNNNRTG 2 cut(s) 401, 2457
SacI GAGCTC 1 cut(s) 2694
SacII CCGCGG 1 cut(s) 2719
Sau3AI GATC 7 cut(s) 473, 786, 1279, 1958, 2227, 2508, 2623
Sau96I GGNCC 6 cut(s) 368, 800, 1432, 1709, 1946, 2713
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 3 cut(s) 662, 1318, 2193
ScrFI CCNGG 5 cut(s) 258, 798, 830, 1953, 2358
SduI GDGCHC 4 cut(s) 597, 1553, 2419, 2694
SfaNI GCATC 7 cut(s) 187, 553, 571, 665, 687, 1651, 2395
SfcI CTRYAG 4 cut(s) 1197, 1997, 2547, 2589
Sfr274I CTCGAG 1 cut(s) 1130
Sfr303I CCGCGG 1 cut(s) 2719
SgrBI CCGCGG 1 cut(s) 2719
SinI GGWCC 5 cut(s) 368, 800, 1432, 1709, 2713
SlaI CTCGAG 1 cut(s) 1130
SmiMI CAYNNNNRTG 2 cut(s) 401, 2457
SmlI CTYRAG 5 cut(s) 607, 937, 1130, 1346, 2123
SmoI CTYRAG 5 cut(s) 607, 937, 1130, 1346, 2123
SpeI ACTAGT 1 cut(s) 1490
SphI GCATGC 2 cut(s) 1299, 1390
SseBI AGGCCT 2 cut(s) 1114, 1222
SsiI CCGC 7 cut(s) 95, 847, 1299, 1982, 2027, 2716, 2718
SspI AATATT 2 cut(s) 965, 2380
SspMI CTAG 9 cut(s) 117, 423, 447, 641, 1491, 1785, 2142, 2442, 2697
SstI GAGCTC 1 cut(s) 2694
StuI AGGCCT 2 cut(s) 1114, 1222
StyD4I CCNGG 5 cut(s) 256, 796, 828, 1951, 2356
StyI CCWWGG 3 cut(s) 309, 2390, 2696
TaqI TCGA 4 cut(s) 597, 1131, 1957, 2469
TatI WGTACW 9 cut(s) 357, 707, 835, 1342, 1822, 2019, 2073, 2449, 2540
TauI GCSGC 2 cut(s) 1302, 2030
TfiI GAWTC 4 cut(s) 413, 946, 2066, 2192
TseFI GTSAC 5 cut(s) 451, 1243, 1514, 1747, 2659
TseI GCWGC 9 cut(s) 441, 515, 613, 620, 1292, 1507, 1979, 2588, 2591
Tsp45I GTSAC 5 cut(s) 451, 1243, 1514, 1747, 2659
TspGWI ACGGA 2 cut(s) 478, 1008
Tth111I GACNNNGTC 1 cut(s) 602
Vha464I CTTAAG 2 cut(s) 607, 2123
VpaK11BI GGWCC 5 cut(s) 368, 800, 1432, 1709, 2713
XapI RAATTY 6 cut(s) 314, 886, 920, 1858, 2129, 2279
XceI RCATGY 2 cut(s) 1299, 1390
XhoI CTCGAG 1 cut(s) 1130
XmaJI CCTAGG 1 cut(s) 2696
XmnI GAANNNNTTC 2 cut(s) 1693, 1858
XspI CTAG 9 cut(s) 117, 423, 447, 641, 1491, 1785, 2142, 2442, 2697
ZrmI AGTACT 1 cut(s) 709
Zsp2I ATGCAT 2 cut(s) 586, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.