Rh2CG514300

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001253
Physical Location & Seq
Reverse (-)
68841899 .. 68846670
4772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG514300.1

Sequence Viewer

Length: 1491 bp
ATGCAATACTATTTGCAGCTTCACATGACTTATGAAGATAATAAGACAGTTCTTCCAGGTATGGGAATTGGGAGAAAATTGATGGATAGGCTTTATCATACATACTCCATGGAATTTGATGGCAAAAGGTTTGCATATGATGGGGAGAAATATCTGTACACATTTGGTCCTCTCCCACTGCACAAGCTCAATTACACAGTTGTGCTGGAGGAATCAGTTTCTAGGCCACATGAAACTGGCAGCTCTAATGTCACTGAAAAACAGATGAAACGATCTATTCAGTCAAAGACTTTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAGCCAATTGCTCTTGCCCTTAAAGGAGCTGATGCAGATGATGGTCAAGATGCATTGAGAGTGCTCGACATAGTCTTAAGGCAGCAAGCAGCTAACAGGGGATGTCTTCTAGTAAGACAGTCATTCCTTCATGATGACTCCAGATGCATCACTGATGTTGGAGGTACCGGTTTAAGTACTGTTCGGGGCTTCCATTCCAGTTTTCGTCCCACTCAGGGTGGCCTGACCTTGAACATGGATGTATCTACTACACTGATCTTAACCCCTGGACAAGTGATTGATTTTCTGAAAGCTAACCAGGGTGTACAACACCCCCGCAACATTGACTGGGCAAGGGCTGTAAGGAATTATGGCTATAATGATGATCCTGTGCTTGCTGCATGCGGTATATCCATAGAGAAGCAATTGGCTCAGATTGAAGGTCGTGTACTTGAGGCTCCGAAGTTGAAGGTAGGCAACAGTTCCGATTGCATGCCTTCAGATGGGAGATGGAACTTCAATGGCAAGACATTTTTGAACCCCAAACACATTGACAAATGGATTGTTGTCAACTTCTCTTTGGAAAATTGTGATACTAGTCGCATCTCAAGGCAGCTTTATGACATTGGGAGGAAGAAGGGCATTAGAATTGAGTGCCCTATCACATTGATTGAGGAACAACCACAGCTTAAAAAACAAAGCCCTATTGAAAGAGTTAAAAAGATGTTTGAACAGATTAAAGTGAAGCATCCTAAAGAGCCACCAAACTTCATTCTTTGTGTTTTACCAGAAAGAAAAACTTCTGATATATATGGACCGTGGAAGAAAGAGTGCCTTGTTGACTATGGCATTCTTGGAGGAATAAATTCTTTGTTGGCAACAGAACACTCTGGGGTTGTTTCTCTAATTAAAGATACTCCTACAATGATTTTGGGGATGGATGTATCTCATGGGCCTCCTGGTCGATCAGATATTCCTTCTGTTGCTGCGGCTTACAAGCACCACCAGAAGGTTGATGAGCTTCCAAAATTTACTGTGATTGTGGCTCAAAAGAACCATAATACAAAGCTATTCCAAGCTGGTTCTCCCCAGTATAATGTTCCACCAGGTACCAGAGGAGTACAACTGCAGTCTCAATTGTTGCACCCGTATGTTATGCGCACCTTGCTGCAGCACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

496

Amino Acids

55.73

Weight (kDa)

9.18

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 21 - 136 3.9e-16 N-terminal domain of argonaute
ArgoL1 PF08699 148 - 196 4.2e-14 Argonaute linker 1 domain
ArgoL2 PF16488 223 - 260 1.2e-11 Argonaute linker 2 domain
ArgoMid PF16487 268 - 322 5.1e-07 Mid domain of argonaute
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1469
Acc65I GGTACC 2 cut(s) 494, 1418
AccB1I GGYRCC 2 cut(s) 494, 1418
AciI CCGC 3 cut(s) 646, 714, 1298
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 3 cut(s) 113, 1174, 1337
AcuI CTGAAG 2 cut(s) 350, 792
AfaI GTAC 7 cut(s) 158, 496, 508, 636, 759, 1420, 1431
AfiI CCNNNNNNNGG 4 cut(s) 62, 545, 812, 1318
AflII CTTAAG 1 cut(s) 406
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 8 cut(s) 295, 562, 749, 778, 829, 847, 1019, 1040
AhlI ACTAGT 1 cut(s) 905
AjnI CCWGG 5 cut(s) 55, 595, 627, 1267, 1414
AleI CACNNNNGTG 1 cut(s) 200
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 1446
AlwI GGATC 1 cut(s) 689
AoxI GGCC 3 cut(s) 224, 550, 1262
ApoI RAATTY 3 cut(s) 113, 1174, 1337
AsiGI ACCGGT 1 cut(s) 497
Asp700I GAANNNNTTC 2 cut(s) 1108, 1174
Asp718I GGTACC 2 cut(s) 494, 1418
AspLEI GCGC 1 cut(s) 1470
AspS9I GGNCC 3 cut(s) 167, 1124, 1262
AvaII GGWCC 2 cut(s) 167, 1124
BaeGI GKGCMC 1 cut(s) 968
BanI GGYRCC 2 cut(s) 494, 1418
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 9 cut(s) 28, 252, 301, 424, 431, 694, 934, 1282, 1464
BccI CCATC 7 cut(s) 76, 113, 134, 365, 806, 813, 1240
BciT130I CCWGG 5 cut(s) 57, 597, 629, 1269, 1416
BcoDI GTCTC 1 cut(s) 1446
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 3 cut(s) 222, 440, 906
BfmI CTRYAG 2 cut(s) 1436, 1478
BfrI CTTAAG 1 cut(s) 406
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 5 cut(s) 57, 597, 629, 1269, 1416
Bme18I GGWCC 2 cut(s) 167, 1124
BmgT120I GGNCC 3 cut(s) 167, 1124, 1262
BmiI GGNNCC 3 cut(s) 496, 768, 1420
BmrFI CCNGG 5 cut(s) 57, 597, 629, 1269, 1416
BmrI ACTGGG 2 cut(s) 667, 1393
BmsI GCATC 6 cut(s) 352, 370, 464, 486, 921, 1066
BmuI ACTGGG 2 cut(s) 667, 1393
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 227, 454
BpuEI CTTGAG 2 cut(s) 782, 901
BsaJI CCNNGG 4 cut(s) 108, 595, 628, 1127
BsaWI WCCGGW 1 cut(s) 497
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 545, 812, 1318
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 4 cut(s) 241, 528, 662, 1399
BseBI CCWGG 5 cut(s) 57, 597, 629, 1269, 1416
BseDI CCNNGG 4 cut(s) 108, 595, 628, 1127
BseGI GGATG 5 cut(s) 437, 574, 1057, 1251, 1255
BseLI CCNNNNNNNGG 4 cut(s) 62, 545, 812, 1318
BseMII CTCAG 2 cut(s) 557, 755
BseNI ACTGG 4 cut(s) 241, 528, 662, 1399
BseRI GAGGAG 1 cut(s) 1440
BseSI GKGCMC 1 cut(s) 968
BseXI GCAGC 9 cut(s) 28, 252, 301, 424, 431, 694, 934, 1282, 1464
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 3 cut(s) 226, 552, 1264
BshNI GGYRCC 2 cut(s) 494, 1418
BshTI ACCGGT 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 4 cut(s) 62, 545, 812, 1318
BsmAI GTCTC 1 cut(s) 1446
BsmFI GGGAC 1 cut(s) 522
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 3 cut(s) 226, 552, 1264
Bsp1286I GDGCHC 2 cut(s) 396, 968
Bsp1407I TGTACA 2 cut(s) 156, 634
Bsp143I GATC 4 cut(s) 272, 585, 694, 1274
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 3 cut(s) 646, 714, 1298
BspANI GGCC 3 cut(s) 226, 552, 1264
BspCNI CTCAG 2 cut(s) 556, 754
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 496, 768, 1420
BspMAI CTGCAG 2 cut(s) 1440, 1482
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 2 cut(s) 494, 1418
BspTI CTTAAG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 2 cut(s) 156, 634
BsrI ACTGG 4 cut(s) 241, 528, 662, 1399
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 4 cut(s) 108, 595, 628, 1127
BssMI GATC 4 cut(s) 272, 585, 694, 1274
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 5 cut(s) 57, 597, 629, 1269, 1416
Bst4CI ACNGT 7 cut(s) 49, 199, 450, 511, 791, 1128, 1345
BstAFI CTTAAG 1 cut(s) 406
BstAUI TGTACA 2 cut(s) 156, 634
BstC8I GCNNGC 4 cut(s) 417, 705, 712, 803
BstDEI CTNAG 2 cut(s) 543, 741
BstDSI CCRYGG 2 cut(s) 108, 1127
BstF5I GGATG 5 cut(s) 437, 574, 1057, 1251, 1255
BstHHI GCGC 1 cut(s) 1470
BstKTI GATC 4 cut(s) 275, 588, 697, 1277
BstMAI GTCTC 1 cut(s) 1446
BstMBI GATC 4 cut(s) 272, 585, 694, 1274
BstMWI GCNNNNNNNGC 1 cut(s) 1474
BstNI CCWGG 5 cut(s) 57, 597, 629, 1269, 1416
BstNSI RCATGY 2 cut(s) 714, 805
BstSCI CCNGG 5 cut(s) 55, 595, 627, 1267, 1414
BstSFI CTRYAG 2 cut(s) 1436, 1478
BstSLI GKGCMC 1 cut(s) 968
BstV1I GCAGC 9 cut(s) 28, 252, 301, 424, 431, 694, 934, 1282, 1464
BstV2I GAAGAC 1 cut(s) 428
BsuRI GGCC 3 cut(s) 226, 552, 1264
BtgI CCRYGG 2 cut(s) 108, 1127
BtsCI GGATG 5 cut(s) 437, 574, 1057, 1251, 1255
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 4 cut(s) 176, 252, 480, 581
Cac8I GCNNGC 4 cut(s) 417, 705, 712, 803
CciI TCATGA 1 cut(s) 460
CfoI GCGC 1 cut(s) 1470
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 167, 1124, 1262
CsiI ACCWGGT 1 cut(s) 1414
Csp6I GTAC 7 cut(s) 157, 495, 507, 635, 758, 1419, 1430
CspAI ACCGGT 1 cut(s) 497
CviAII CATG 8 cut(s) 25, 109, 230, 461, 565, 711, 802, 1259
CviQI GTAC 7 cut(s) 157, 495, 507, 635, 758, 1419, 1430
DdeI CTNAG 2 cut(s) 543, 741
DpnI GATC 4 cut(s) 274, 587, 696, 1276
DpnII GATC 4 cut(s) 272, 585, 694, 1274
Eco130I CCWWGG 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 167, 1124
Eco57I CTGAAG 2 cut(s) 350, 792
EcoRII CCWGG 5 cut(s) 55, 595, 627, 1267, 1414
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 2 cut(s) 385, 479
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 8 cut(s) 28, 112, 233, 464, 568, 714, 805, 1262
FalI AAGNNNNNCTT 4 cut(s) 1093, 1125, 1128, 1160
FaqI GGGAC 1 cut(s) 522
FatI CATG 8 cut(s) 24, 108, 229, 460, 564, 710, 801, 1258
FauI CCCGC 1 cut(s) 653
FauNDI CATATG 1 cut(s) 136
FokI GGATG 5 cut(s) 444, 581, 1044, 1258, 1262
FspAI RTGCGCAY 1 cut(s) 1469
FspBI CTAG 3 cut(s) 222, 440, 906
FspI TGCGCA 1 cut(s) 1469
GlaI GCGC 1 cut(s) 1469
GsuI CTGGAG 2 cut(s) 227, 454
HaeIII GGCC 3 cut(s) 226, 552, 1264
HapII CCGG 1 cut(s) 498
HhaI GCGC 1 cut(s) 1470
Hin1II CATG 8 cut(s) 28, 112, 233, 464, 568, 714, 805, 1262
Hin6I GCGC 1 cut(s) 1468
HinP1I GCGC 1 cut(s) 1468
HincII GTYRAC 2 cut(s) 880, 1150
HindII GTYRAC 2 cut(s) 880, 1150
HinfI GANTC 2 cut(s) 212, 467
HpaII CCGG 1 cut(s) 498
Hpy166II GTNNAC 5 cut(s) 159, 635, 758, 880, 1150
Hpy188I TCNGA 8 cut(s) 330, 618, 744, 771, 796, 811, 1114, 1279
Hpy188III TCNNGA 3 cut(s) 377, 461, 471
Hpy8I GTNNAC 5 cut(s) 159, 635, 758, 880, 1150
HpyAV CCTTC 7 cut(s) 467, 743, 772, 816, 940, 1296, 1312
HpyCH4III ACNGT 7 cut(s) 49, 199, 450, 511, 791, 1128, 1345
HpyF10VI GCNNNNNNNGC 1 cut(s) 1474
HpyF3I CTNAG 2 cut(s) 543, 741
Hsp92II CATG 8 cut(s) 28, 112, 233, 464, 568, 714, 805, 1262
HspAI GCGC 1 cut(s) 1468
KpnI GGTACC 2 cut(s) 498, 1422
Kzo9I GATC 4 cut(s) 272, 585, 694, 1274
LmnI GCTCC 2 cut(s) 356, 772
Lsp1109I GCAGC 9 cut(s) 28, 252, 301, 424, 431, 694, 934, 1282, 1464
LweI GCATC 6 cut(s) 352, 370, 464, 486, 921, 1066
MabI ACCWGGT 1 cut(s) 1414
MaeI CTAG 3 cut(s) 222, 440, 906
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 274, 587, 696, 1276
MboI GATC 4 cut(s) 272, 585, 694, 1274
MboII GAAGA 5 cut(s) 44, 47, 428, 955, 1144
MfeI CAATTG 3 cut(s) 336, 734, 1445
MhlI GDGCHC 2 cut(s) 396, 968
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 469
Mph1103I ATGCAT 2 cut(s) 385, 479
MroXI GAANNNNTTC 2 cut(s) 1108, 1174
MseI TTAA 8 cut(s) 351, 407, 503, 590, 999, 1026, 1047, 1218
MslI CAYNNNNRTG 2 cut(s) 200, 1458
MspCI CTTAAG 1 cut(s) 406
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 5 cut(s) 57, 597, 629, 1269, 1416
MunI CAATTG 3 cut(s) 336, 734, 1445
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 5 cut(s) 57, 597, 629, 1269, 1416
MwoI GCNNNNNNNGC 1 cut(s) 1474
NcoI CCATGG 1 cut(s) 108
NdeI CATATG 1 cut(s) 136
NdeII GATC 4 cut(s) 272, 585, 694, 1274
NlaIII CATG 8 cut(s) 28, 112, 233, 464, 568, 714, 805, 1262
NlaIV GGNNCC 3 cut(s) 496, 768, 1420
NmuCI GTSAC 1 cut(s) 250
NsbI TGCGCA 1 cut(s) 1469
NsiI ATGCAT 2 cut(s) 385, 479
NspI RCATGY 2 cut(s) 714, 805
OliI CACNNNNGTG 1 cut(s) 200
PaeI GCATGC 2 cut(s) 714, 805
PagI TCATGA 1 cut(s) 460
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 2 cut(s) 1108, 1174
PfeI GAWTC 1 cut(s) 212
PflFI GACNNNGTC 1 cut(s) 401
PinAI ACCGGT 1 cut(s) 497
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Psp6I CCWGG 5 cut(s) 55, 595, 627, 1267, 1414
PspGI CCWGG 5 cut(s) 55, 595, 627, 1267, 1414
PspN4I GGNNCC 3 cut(s) 496, 768, 1420
PspPI GGNCC 3 cut(s) 167, 1124, 1262
PstI CTGCAG 2 cut(s) 1440, 1482
PsyI GACNNNGTC 1 cut(s) 401
RsaI GTAC 7 cut(s) 158, 496, 508, 636, 759, 1420, 1431
RsaNI GTAC 7 cut(s) 157, 495, 507, 635, 758, 1419, 1430
RseI CAYNNNNRTG 2 cut(s) 200, 1458
SaqAI TTAA 8 cut(s) 351, 407, 503, 590, 999, 1026, 1047, 1218
Sau3AI GATC 4 cut(s) 272, 585, 694, 1274
Sau96I GGNCC 3 cut(s) 167, 1124, 1262
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 5 cut(s) 57, 597, 629, 1269, 1416
SduI GDGCHC 2 cut(s) 396, 968
SexAI ACCWGGT 1 cut(s) 1414
SfaNI GCATC 6 cut(s) 352, 370, 464, 486, 921, 1066
SfcI CTRYAG 2 cut(s) 1436, 1478
SinI GGWCC 2 cut(s) 167, 1124
SmiMI CAYNNNNRTG 2 cut(s) 200, 1458
SmlI CTYRAG 3 cut(s) 406, 761, 916
SmoI CTYRAG 3 cut(s) 406, 761, 916
SpeI ACTAGT 1 cut(s) 905
SphI GCATGC 2 cut(s) 714, 805
SsiI CCGC 3 cut(s) 646, 714, 1298
SspMI CTAG 3 cut(s) 222, 440, 906
StyD4I CCNGG 5 cut(s) 55, 595, 627, 1267, 1414
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 7 cut(s) 49, 199, 450, 511, 791, 1128, 1345
TaqI TCGA 2 cut(s) 396, 1273
TatI WGTACW 5 cut(s) 156, 506, 634, 757, 1429
TauI GCSGC 1 cut(s) 1301
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 8 cut(s) 351, 407, 503, 590, 999, 1026, 1047, 1218
Tru9I TTAA 8 cut(s) 351, 407, 503, 590, 999, 1026, 1047, 1218
TscAI CASTG 4 cut(s) 183, 259, 487, 588
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 48, 246, 281, 449, 1069
TspRI CASTG 4 cut(s) 183, 259, 487, 588
Tth111I GACNNNGTC 1 cut(s) 401
Vha464I CTTAAG 1 cut(s) 406
VpaK11BI GGWCC 2 cut(s) 167, 1124
XapI RAATTY 3 cut(s) 113, 1174, 1337
XceI RCATGY 2 cut(s) 714, 805
XmnI GAANNNNTTC 2 cut(s) 1108, 1174
XspI CTAG 3 cut(s) 222, 440, 906
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 2 cut(s) 385, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.