FvH4_5g00540

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001109
Physical Location & Seq
Reverse (-)
338134 .. 339481
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00540.t1

Sequence Viewer

Length: 828 bp
ATGGGATTCATGGAAATAGTTGCTTCTGACGAGGGACTAGGAACGATTGTCCCGATCGTAGTGTACTGGCTGTACGCAGCCCTCATTATATCTATGGAGAAATCATTTCCTAAACACAAAATTCGAAGCGCAGAGGAGCAACACAACAAAAACCACCTCGTCTCCAAAGGGACAGTCATCAAAGGCGTCATTCTCCAGCAAGCATTGCAGGCTTTCACTGCCACACTCTTGTATATTCTGATTGAAAGTGACGATGATAGTGCTGCTCATGTGACTACTTCTAATACAGGTCAAAATCATGATGGTGACGATGGAACTGGGTCAAAGATCATAACGATGCTTGTCAGAACTGCATTCCAGTTTCTGAAAGGCATGGTGGTGATGGATACATGGCAGTACTTCATGCACAGGTATATGCATGAGAACAAGTTCCTCTACAAGCACGTCCACTCCCAGCATCACAGGCTTGTAGCTCCGTATGCATATGCAGCATTGTACAACCACCCGTTGGAGGGTCTTCTCGTGGACACTATGGGTGGAGCCGTCAGCTTCTTGGCGTCGGGGATGTCTCCACGGACTTCGATTTTCTTCTTCTCATTCGCAACCATCAAGGCGGTCGACGAGCATTCCGGTGTAAACTTTCCATGGCACCCGTTTCGTCTTCTGTTCAGAAACAACAGCCTGTATCATGATGTGCACCACCAGCTTTACGGCACCAAGTTCAACTTCTCTCAGCCCTTCTTTGTTTTTTGGGATAGGTTACTTGGTACCTACCATATGCCATCATCTACTGAAGACGAGCTCAACAAGGGGGCCAAGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

276

Amino Acids

31.31

Weight (kDa)

6.64

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 122 - 257 3.4e-26 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 3 cut(s) 648, 713, 767
AccB7I CCANNNNNTGG 1 cut(s) 508
AccI GTMKAC 1 cut(s) 618
AciI CCGC 1 cut(s) 614
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 813
AcyI GRCGYC 2 cut(s) 186, 557
AfaI GTAC 5 cut(s) 65, 74, 398, 497, 769
AfiI CCNNNNNNNGG 3 cut(s) 508, 511, 512
AgsI TTSAA 2 cut(s) 245, 724
AjiI CACGTC 1 cut(s) 445
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AluBI AGCT 4 cut(s) 473, 549, 706, 802
AluI AGCT 4 cut(s) 473, 549, 706, 802
Alw21I GWGCWC 2 cut(s) 699, 804
Alw26I GTCTC 2 cut(s) 166, 573
Alw44I GTGCAC 1 cut(s) 695
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 1 cut(s) 813
ApaLI GTGCAC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 77, 263, 488
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 2 cut(s) 159, 191
Asp700I GAANNNNTTC 1 cut(s) 428
Asp718I GGTACC 1 cut(s) 767
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 813
AsuHPI GGTGA 2 cut(s) 317, 391
AsuII TTCGAA 1 cut(s) 124
BaeGI GKGCMC 1 cut(s) 699
BanI GGYRCC 3 cut(s) 648, 713, 767
BanII GRGCYC 1 cut(s) 804
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 3 cut(s) 509, 653, 801
Bbv12I GWGCWC 2 cut(s) 699, 804
BbvI GCAGC 3 cut(s) 89, 250, 500
BccI CCATC 5 cut(s) 296, 305, 376, 614, 790
BceAI ACGGC 2 cut(s) 527, 727
BcgI CGANNNNNNTGC 4 cut(s) 242, 276, 638, 672
BciVI GTATCC 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 166, 573
BfaI CTAG 1 cut(s) 38
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 3 cut(s) 78, 264, 489
BlsI GCNGC 3 cut(s) 79, 265, 490
BmcAI AGTACT 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 813
BmiI GGNNCC 5 cut(s) 541, 650, 715, 769, 814
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 327, 466
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 3 cut(s) 509, 653, 801
BpmI CTGGAG 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 124
BsaHI GRCGYC 2 cut(s) 186, 557
BsaJI CCNNGG 3 cut(s) 572, 644, 816
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 508, 511, 512
Bse1I ACTGG 3 cut(s) 71, 322, 358
Bse3DI GCAATG 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 572, 644, 816
BseGI GGATG 1 cut(s) 570
BseLI CCNNNNNNNGG 3 cut(s) 508, 511, 512
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 3 cut(s) 71, 322, 358
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 699
BseXI GCAGC 3 cut(s) 89, 250, 500
BseYI CCCAGC 1 cut(s) 453
Bsh1285I CGRYCG 2 cut(s) 57, 618
BshFI GGCC 1 cut(s) 815
BshNI GGYRCC 3 cut(s) 648, 713, 767
BsiEI CGRYCG 2 cut(s) 57, 618
BsiHKAI GWGCWC 2 cut(s) 699, 804
BsiSI CCGG 1 cut(s) 630
BslFI GGGAC 3 cut(s) 35, 48, 184
BslI CCNNNNNNNGG 3 cut(s) 508, 511, 512
BsmAI GTCTC 2 cut(s) 166, 573
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 3 cut(s) 35, 48, 184
BsmI GAATGC 2 cut(s) 353, 625
BsnI GGCC 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 699, 804
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 2 cut(s) 54, 327
Bsp19I CCATGG 1 cut(s) 644
BspACI CCGC 1 cut(s) 614
BspANI GGCC 1 cut(s) 815
BspCNI CTCAG 1 cut(s) 745
BspHI TCATGA 2 cut(s) 298, 688
BspLI GGNNCC 5 cut(s) 541, 650, 715, 769, 814
BspT104I TTCGAA 1 cut(s) 124
BspT107I GGYRCC 3 cut(s) 648, 713, 767
BsrDI GCAATG 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 3 cut(s) 71, 322, 358
BssECI CCNNGG 3 cut(s) 572, 644, 816
BssMI GATC 2 cut(s) 54, 327
BssNI GRCGYC 2 cut(s) 186, 557
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 2 cut(s) 644, 816
Bst2BI CACGAG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 175
BstACI GRCGYC 2 cut(s) 186, 557
BstAPI GCANNNNNTGC 1 cut(s) 205
BstAUI TGTACA 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 201, 210
BstDEI CTNAG 1 cut(s) 732
BstDSI CCRYGG 2 cut(s) 572, 644
BstF5I GGATG 1 cut(s) 570
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 57, 330
BstMAI GTCTC 2 cut(s) 166, 573
BstMBI GATC 2 cut(s) 54, 327
BstMCI CGRYCG 2 cut(s) 57, 618
BstMWI GCNNNNNNNGC 7 cut(s) 205, 209, 218, 463, 479, 488, 703
BstSLI GKGCMC 1 cut(s) 699
BstV1I GCAGC 3 cut(s) 89, 250, 500
BstV2I GAAGAC 3 cut(s) 509, 653, 801
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 815
BtgI CCRYGG 2 cut(s) 572, 644
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 1 cut(s) 570
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 201, 210
CaiI CAGNNNCTG 1 cut(s) 364
CciI TCATGA 2 cut(s) 298, 688
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 813
CseI GACGC 2 cut(s) 175, 546
Csp6I GTAC 5 cut(s) 64, 73, 397, 496, 768
CviAII CATG 9 cut(s) 10, 269, 299, 373, 390, 403, 419, 645, 689
CviQI GTAC 5 cut(s) 64, 73, 397, 496, 768
DdeI CTNAG 1 cut(s) 732
DpnI GATC 2 cut(s) 56, 329
DpnII GATC 2 cut(s) 54, 327
Ecl136II GAGCTC 1 cut(s) 802
Eco130I CCWWGG 2 cut(s) 644, 816
Eco24I GRGCYC 1 cut(s) 804
Eco53kI GAGCTC 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 813
EcoICRI GAGCTC 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 644, 816
EcoT22I ATGCAT 2 cut(s) 420, 484
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 2 cut(s) 644, 816
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 9 cut(s) 13, 272, 302, 376, 393, 406, 422, 648, 692
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 3 cut(s) 35, 48, 184
FatI CATG 9 cut(s) 9, 268, 298, 372, 389, 402, 418, 644, 688
FauNDI CATATG 2 cut(s) 484, 777
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 3 cut(s) 78, 264, 489
FokI GGATG 1 cut(s) 577
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 3 cut(s) 78, 264, 489
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 130
GluI GCNGC 3 cut(s) 78, 264, 489
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 815
HapII CCGG 1 cut(s) 630
HgaI GACGC 2 cut(s) 175, 546
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 2 cut(s) 186, 557
Hin1II CATG 9 cut(s) 13, 272, 302, 376, 393, 406, 422, 648, 692
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 1 cut(s) 6
HpaII CCGG 1 cut(s) 630
HphI GGTGA 2 cut(s) 317, 391
Hpy166II GTNNAC 6 cut(s) 64, 448, 526, 619, 637, 697
Hpy188I TCNGA 5 cut(s) 28, 240, 347, 366, 671
Hpy188III TCNNGA 3 cut(s) 52, 299, 689
Hpy8I GTNNAC 6 cut(s) 64, 448, 526, 619, 637, 697
Hpy99I CGWCG 2 cut(s) 562, 623
HpyAV CCTTC 1 cut(s) 748
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 7 cut(s) 208, 353, 406, 418, 482, 488, 697
HpyF10VI GCNNNNNNNGC 7 cut(s) 205, 209, 218, 463, 479, 488, 703
HpyF3I CTNAG 1 cut(s) 732
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 2 cut(s) 186, 557
Hsp92II CATG 9 cut(s) 13, 272, 302, 376, 393, 406, 422, 648, 692
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 771
Kzo9I GATC 2 cut(s) 54, 327
LmnI GCTCC 3 cut(s) 136, 478, 539
Lsp1109I GCAGC 3 cut(s) 89, 250, 500
LweI GCATC 2 cut(s) 327, 466
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 4 cut(s) 248, 271, 305, 759
MalI GATC 2 cut(s) 56, 329
MboI GATC 2 cut(s) 54, 327
MboII GAAGA 5 cut(s) 509, 580, 583, 653, 806
MhlI GDGCHC 2 cut(s) 699, 804
MluCI AATT 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 6 cut(s) 25, 92, 127, 167, 443, 505
Mph1103I ATGCAT 2 cut(s) 420, 484
MroXI GAANNNNTTC 1 cut(s) 428
MslI CAYNNNNRTG 4 cut(s) 303, 335, 377, 630
MspI CCGG 1 cut(s) 630
Mva1269I GAATGC 2 cut(s) 353, 625
MwoI GCNNNNNNNGC 7 cut(s) 205, 209, 218, 463, 479, 488, 703
NcoI CCATGG 1 cut(s) 644
NdeI CATATG 2 cut(s) 484, 777
NdeII GATC 2 cut(s) 54, 327
NlaIII CATG 9 cut(s) 13, 272, 302, 376, 393, 406, 422, 648, 692
NlaIV GGNNCC 5 cut(s) 541, 650, 715, 769, 814
NmuCI GTSAC 3 cut(s) 248, 271, 305
NsiI ATGCAT 2 cut(s) 420, 484
NspV TTCGAA 1 cut(s) 124
PagI TCATGA 2 cut(s) 298, 688
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 2 cut(s) 353, 625
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 3 cut(s) 79, 265, 490
Ple19I CGATCG 1 cut(s) 57
Psp124BI GAGCTC 1 cut(s) 804
PspFI CCCAGC 1 cut(s) 453
PspN4I GGNNCC 5 cut(s) 541, 650, 715, 769, 814
PspPI GGNCC 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 364
PvuI CGATCG 1 cut(s) 57
RsaI GTAC 5 cut(s) 65, 74, 398, 497, 769
RsaNI GTAC 5 cut(s) 64, 73, 397, 496, 768
RseI CAYNNNNRTG 4 cut(s) 303, 335, 377, 630
SacI GAGCTC 1 cut(s) 804
SalI GTCGAC 1 cut(s) 617
SatI GCNGC 3 cut(s) 78, 264, 489
Sau3AI GATC 2 cut(s) 54, 327
Sau96I GGNCC 1 cut(s) 813
ScaI AGTACT 1 cut(s) 398
SduI GDGCHC 2 cut(s) 699, 804
SfaNI GCATC 2 cut(s) 327, 466
SfuI TTCGAA 1 cut(s) 124
SmiMI CAYNNNNRTG 4 cut(s) 303, 335, 377, 630
Sse9I AATT 1 cut(s) 120
SsiI CCGC 1 cut(s) 614
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 804
StyI CCWWGG 2 cut(s) 644, 816
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 447
TaqI TCGA 3 cut(s) 124, 581, 618
TasI AATT 1 cut(s) 120
TatI WGTACW 3 cut(s) 63, 396, 495
TfiI GAWTC 1 cut(s) 6
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 3 cut(s) 248, 271, 305
TseI GCWGC 3 cut(s) 77, 263, 488
Tsp45I GTSAC 3 cut(s) 248, 271, 305
TspDTI ATGAA 1 cut(s) 391
TspGWI ACGGA 2 cut(s) 465, 589
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 508
VneI GTGCAC 1 cut(s) 695
XapI RAATTY 1 cut(s) 120
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 2 cut(s) 420, 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.