MD10G1041400.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001109
Physical Location & Seq
Reverse (-)
5382980 .. 5386477
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1041400.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGTGTTCTGGGAAGGTTATGTGAGTGATGAGGTAATGGGGACTTTTGCCCCAATTGTGGTTTACTGGTTGTATGCGGGGGCTTACCAGTTGTTGCCGCCCTTGGACAATTACCGATTGCATTCAAGAAGAGAGGAAGACGAGAAGAACTCAATGCCTCTCCCCTCTGTTGTTAAGGGTGTTTTGCTTCAACAGCTCGTCCAGGCCACCGTTGCTCAGGGGCTCTTCTTGTTGACCTCGAAGGCAAATATCTCCGGGGTCACAATTCAGCCCTCGATACCTGTGCAGATTGTCCAGATTGTTATCGCAATGCTAGTAATGGATACATGGCAGTACTTTGTGCACCGCTACATGCATCAGAACAAGTTTTTGTATCGCCATGTCCACTCTCAGCACCACAGACTCGTTGTTCCATATGCAATTGGAGCCCTATATAATCACCCACTTGAGGGTCTCTTGCTCGACACTTTTGGCGGGGCTCTCTCGTTTCTAGTCTCAGGGATGACAGCACGAACGGCTGTTATATTCTTCTGCTTTGCCGTGATTAAAACTGTCGATGATCACTGTGGGCTTTGGTTGCCTGGTAATATATTCCATCTTCTATTCCAGAACAACACTGCTTATCATGACATACACCATCAACTTCAAGGCTTGAAGTACAACTATTCCCAGCCATTTTTCCCGATATGGGATAAACTTTTTGGCACCTACATGCCGTATAATCTTGTCAAGAGACCTGAAGGGGGGTTTGAAGCGAGGGCAATGAAGGCAATGAAAGACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

262

Amino Acids

29.93

Weight (kDa)

7.84

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 101 - 236 1.3e-26 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AciI CCGC 4 cut(s) 77, 98, 346, 474
AcuI CTGAAG 1 cut(s) 759
AfaI GTAC 2 cut(s) 335, 659
AfiI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
AgsI TTSAA 5 cut(s) 126, 191, 647, 655, 752
AjnI CCWGG 2 cut(s) 201, 580
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 3 cut(s) 458, 499, 727
Alw44I GTGCAC 1 cut(s) 341
AoxI GGCC 1 cut(s) 204
ApaLI GTGCAC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 3 cut(s) 225, 430, 481
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 345
BccI CCATC 2 cut(s) 603, 645
BceAI ACGGC 3 cut(s) 524, 531, 700
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 2 cut(s) 203, 582
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 458, 499, 727
BfaI CTAG 2 cut(s) 314, 491
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 203, 256, 582
BmiI GGNNCC 2 cut(s) 427, 706
BmrFI CCNGG 3 cut(s) 203, 256, 582
BmsI GCATC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 302
BsaI GGTCTC 2 cut(s) 458, 727
BsaJI CCNNGG 2 cut(s) 102, 255
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
Bse1I ACTGG 2 cut(s) 71, 88
Bse3DI GCAATG 3 cut(s) 315, 768, 777
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 2 cut(s) 203, 582
BseDI CCNNGG 2 cut(s) 102, 255
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
BseMI GCAATG 3 cut(s) 315, 768, 777
BseMII CTCAG 3 cut(s) 230, 404, 510
BseNI ACTGG 2 cut(s) 71, 88
BseSI GKGCMC 1 cut(s) 345
BseYI CCCAGC 1 cut(s) 669
BsgI GTGCAG 1 cut(s) 305
BshFI GGCC 1 cut(s) 206
BshNI GGYRCC 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
BsmAI GTCTC 3 cut(s) 458, 499, 727
BsmFI GGGAC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 1 cut(s) 206
Bso31I GGTCTC 2 cut(s) 458, 727
Bsp1286I GDGCHC 4 cut(s) 225, 345, 430, 481
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 4 cut(s) 77, 98, 346, 474
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 3 cut(s) 229, 403, 509
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 2 cut(s) 427, 706
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 704
BspTNI GGTCTC 2 cut(s) 458, 727
BsrDI GCAATG 3 cut(s) 315, 768, 777
BsrI ACTGG 2 cut(s) 71, 88
BssECI CCNNGG 2 cut(s) 102, 255
BssMI GATC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 203, 582
Bst4CI ACNGT 4 cut(s) 211, 553, 566, 782
Bst6I CTCTTC 2 cut(s) 124, 230
BstDEI CTNAG 3 cut(s) 216, 390, 496
BstF5I GGATG 1 cut(s) 507
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 3 cut(s) 458, 499, 727
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
BstNI CCWGG 2 cut(s) 203, 582
BstNSI RCATGY 2 cut(s) 355, 715
BstSCI CCNGG 3 cut(s) 201, 254, 580
BstSLI GKGCMC 1 cut(s) 345
BstV2I GAAGAC 1 cut(s) 144
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 562, 615
CciI TCATGA 1 cut(s) 625
Csp6I GTAC 2 cut(s) 334, 658
CviAII CATG 5 cut(s) 327, 352, 380, 626, 712
CviQI GTAC 2 cut(s) 334, 658
DdeI CTNAG 3 cut(s) 216, 390, 496
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
Eam1104I CTCTTC 2 cut(s) 124, 230
EarI CTCTTC 2 cut(s) 124, 230
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 3 cut(s) 225, 430, 481
Eco31I GGTCTC 2 cut(s) 458, 727
Eco57I CTGAAG 1 cut(s) 759
EcoRII CCWGG 2 cut(s) 201, 580
EcoT14I CCWWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 3 cut(s) 225, 430, 481
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 5 cut(s) 330, 355, 383, 629, 715
FaqI GGGAC 1 cut(s) 55
FatI CATG 5 cut(s) 326, 351, 379, 625, 711
FauI CCCGC 2 cut(s) 70, 467
FauNDI CATATG 1 cut(s) 415
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 514
FriOI GRGCYC 3 cut(s) 225, 430, 481
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 2 cut(s) 314, 491
GluI GCNGC 1 cut(s) 98
GsaI CCCAGC 1 cut(s) 673
HaeIII GGCC 1 cut(s) 206
HapII CCGG 1 cut(s) 255
Hin1II CATG 5 cut(s) 330, 355, 383, 629, 715
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 1 cut(s) 402
HpaII CCGG 1 cut(s) 255
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 4 cut(s) 64, 234, 343, 385
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 6 cut(s) 126, 295, 607, 626, 682, 730
Hpy8I GTNNAC 4 cut(s) 64, 234, 343, 385
HpyAV CCTTC 4 cut(s) 8, 235, 734, 760
HpyCH4III ACNGT 4 cut(s) 211, 553, 566, 782
HpyCH4V TGCA 5 cut(s) 121, 286, 343, 355, 419
HpyF10VI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
HpyF3I CTNAG 3 cut(s) 216, 390, 496
Hsp92II CATG 5 cut(s) 330, 355, 383, 629, 715
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 1 cut(s) 559
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 425
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 314, 491
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 6 cut(s) 141, 149, 157, 217, 520, 590
MfeI CAATTG 2 cut(s) 54, 420
MhlI GDGCHC 4 cut(s) 225, 345, 430, 481
MluCI AATT 4 cut(s) 54, 109, 264, 420
MlyI GAGTC 1 cut(s) 396
MnlI CCTC 8 cut(s) 25, 127, 168, 175, 247, 283, 442, 750
Mph1103I ATGCAT 1 cut(s) 357
MseI TTAA 2 cut(s) 174, 546
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 3 cut(s) 203, 256, 582
MunI CAATTG 2 cut(s) 54, 420
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 2 cut(s) 203, 582
MwoI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
NciI CCSGG 1 cut(s) 256
NdeI CATATG 1 cut(s) 415
NdeII GATC 1 cut(s) 559
NlaIII CATG 5 cut(s) 330, 355, 383, 629, 715
NlaIV GGNNCC 2 cut(s) 427, 706
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 2 cut(s) 355, 715
PagI TCATGA 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 230
PctI GAATGC 1 cut(s) 121
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 201, 580
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 201, 580
PspN4I GGNNCC 2 cut(s) 427, 706
RsaI GTAC 2 cut(s) 335, 659
RsaNI GTAC 2 cut(s) 334, 658
RseI CAYNNNNRTG 1 cut(s) 710
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 2 cut(s) 174, 546
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 1 cut(s) 559
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 3 cut(s) 203, 256, 582
SduI GDGCHC 4 cut(s) 225, 345, 430, 481
SetI ASST 7 cut(s) 19, 36, 198, 239, 283, 710, 739
SfaNI GCATC 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 4 cut(s) 54, 109, 264, 420
SsiI CCGC 4 cut(s) 77, 98, 346, 474
SspMI CTAG 2 cut(s) 314, 491
StyD4I CCNGG 3 cut(s) 201, 254, 580
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 4 cut(s) 211, 553, 566, 782
TaqI TCGA 4 cut(s) 239, 275, 462, 555
TasI AATT 4 cut(s) 54, 109, 264, 420
TatI WGTACW 2 cut(s) 333, 657
TauI GCSGC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 174, 546
Tru9I TTAA 2 cut(s) 174, 546
TscAI CASTG 2 cut(s) 569, 622
TseFI GTSAC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 779
TspRI CASTG 2 cut(s) 569, 622
VneI GTGCAC 1 cut(s) 341
XceI RCATGY 2 cut(s) 355, 715
XspI CTAG 2 cut(s) 314, 491
ZrmI AGTACT 1 cut(s) 335
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.