pycom05g02770

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001109
Physical Location & Seq
Reverse (-)
3276302 .. 3279415
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02770.2

Sequence Viewer

Length: 783 bp
ATGGCGTTTTGGGAAGGTTATGTGAGTGATGAGGCAATGGGGACCTTTGCCCCAATTGTGGTTTACTGGTTGTATGCGGGGGTTTACCAGTTGTTGCCGCCTTTGGACAATTACCGCTTGCATACAAGAAGAGAGGAAGACGAGAAGAACTCGGTGCCTCTCCCCTCTGTCGTTAAAGGTGTTTTGCTTCAACAGCTCGTCCAGGCCACCGTTGCTCAGGGGCTTTTTTTGTTGACCTCGAAGGCGAATACCTCGGGGATCACAATTCAGCCCTCGATAACTGTGCAGATTGTCCAGATTGTTATCGCAATGCTAGTAATGGACACATGGCAGTTCTTTGTGCACCGTTACATGCATCAGAACAAATTTTTATACCGCCATGTCCACTCTCAGCACCACAGACTCGTTGTTCCCTACGCAATTGGAGCCCTTTATAATCACCCACTTGAGGGTCTCTTGCTCGACACTTTTGGTGGGGCACTCTCGTTTCTAGTCTCAGGGATGACAGCACGAACGGCTGTTATTTTCTTCTGCTTTGCCGTGATTAAAACTGTTGATGATCGATGTGGGCTTTGGTTGCCTGGTAATATATTCCATCTTTTATTCCAGAACAACACTGCTTATCATGACATACACCATCAACTTCAAGGCCTGAAGTACAACTACTCCCAGCCCTTCTTCCCGATATGGGATAAACTTTTTGGCACCTACATGCCGTATAATCTTGTCAAGAGACCGGAAGGGGGGTTTGAGGCGAGGGCAATGAAGGCAATGAAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

261

Amino Acids

29.8

Weight (kDa)

8.44

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
AccB1I GGYRCC 2 cut(s) 154, 704
AciI CCGC 4 cut(s) 77, 98, 115, 376
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 674
AfaI GTAC 1 cut(s) 659
AfiI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
AgsI TTSAA 2 cut(s) 191, 647
AjnI CCWGG 2 cut(s) 201, 580
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 3 cut(s) 458, 499, 727
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 253
AoxI GGCC 2 cut(s) 204, 649
ApaLI GTGCAC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 253
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 2 cut(s) 345, 481
BanI GGYRCC 2 cut(s) 154, 704
BanII GRGCYC 1 cut(s) 430
BarI GAAGNNNNNNTAC 2 cut(s) 647, 679
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 345
BccI CCATC 2 cut(s) 603, 645
BceAI ACGGC 3 cut(s) 524, 531, 700
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 2 cut(s) 203, 582
BcoDI GTCTC 3 cut(s) 458, 499, 727
BfaI CTAG 3 cut(s) 314, 491, 781
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 203, 582
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 4 cut(s) 43, 156, 427, 706
BmrFI CCNGG 2 cut(s) 203, 582
BmsI GCATC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 467
Bsa29I ATCGAT 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 302
BsaI GGTCTC 2 cut(s) 458, 727
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
Bse1I ACTGG 2 cut(s) 71, 88
Bse3DI GCAATG 4 cut(s) 42, 315, 768, 777
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 2 cut(s) 203, 582
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
BseMI GCAATG 4 cut(s) 42, 315, 768, 777
BseMII CTCAG 3 cut(s) 230, 404, 510
BseNI ACTGG 2 cut(s) 71, 88
BseSI GKGCMC 2 cut(s) 345, 481
BseYI CCCAGC 1 cut(s) 669
BsgI GTGCAG 1 cut(s) 305
BshFI GGCC 2 cut(s) 206, 651
BshNI GGYRCC 2 cut(s) 154, 704
BshVI ATCGAT 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 1 cut(s) 737
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 5 cut(s) 58, 448, 449, 688, 743
BsmAI GTCTC 3 cut(s) 458, 499, 727
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 2 cut(s) 206, 651
Bso31I GGTCTC 2 cut(s) 458, 727
BsoBI CYCGRG 1 cut(s) 253
Bsp1286I GDGCHC 3 cut(s) 345, 430, 481
Bsp143I GATC 2 cut(s) 258, 559
BspACI CCGC 4 cut(s) 77, 98, 115, 376
BspANI GGCC 2 cut(s) 206, 651
BspCNI CTCAG 3 cut(s) 229, 403, 509
BspDI ATCGAT 1 cut(s) 562
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 4 cut(s) 43, 156, 427, 706
BspPI GGATC 1 cut(s) 266
BspT107I GGYRCC 2 cut(s) 154, 704
BspTNI GGTCTC 2 cut(s) 458, 727
BsrDI GCAATG 4 cut(s) 42, 315, 768, 777
BsrI ACTGG 2 cut(s) 71, 88
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 258, 559
Bst2UI CCWGG 2 cut(s) 203, 582
Bst4CI ACNGT 4 cut(s) 211, 283, 347, 553
Bst6I CTCTTC 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 216, 390, 496
BstF5I GGATG 1 cut(s) 507
BstKTI GATC 2 cut(s) 261, 562
BstMAI GTCTC 3 cut(s) 458, 499, 727
BstMBI GATC 2 cut(s) 258, 559
BstMWI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
BstNI CCWGG 2 cut(s) 203, 582
BstNSI RCATGY 2 cut(s) 355, 715
BstSCI CCNGG 2 cut(s) 201, 580
BstSLI GKGCMC 2 cut(s) 345, 481
BstV2I GAAGAC 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 562
BsuRI GGCC 2 cut(s) 206, 651
BsuTUI ATCGAT 1 cut(s) 562
BtsCI GGATG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 1 cut(s) 615
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 625
Cfr13I GGNCC 1 cut(s) 42
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 1 cut(s) 658
CviAII CATG 5 cut(s) 327, 352, 380, 626, 712
CviJI RGCY 9 cut(s) 196, 206, 223, 271, 428, 518, 571, 651, 673
CviKI_1 RGCY 9 cut(s) 196, 206, 223, 271, 428, 518, 571, 651, 673
CviQI GTAC 1 cut(s) 658
DdeI CTNAG 3 cut(s) 216, 390, 496
DpnI GATC 2 cut(s) 260, 561
DpnII GATC 2 cut(s) 258, 559
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco147I AGGCCT 1 cut(s) 651
Eco24I GRGCYC 1 cut(s) 430
Eco31I GGTCTC 2 cut(s) 458, 727
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 201, 580
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 430
FaeI CATG 5 cut(s) 330, 355, 383, 629, 715
FaqI GGGAC 1 cut(s) 55
FatI CATG 5 cut(s) 326, 351, 379, 625, 711
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 514
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 3 cut(s) 314, 491, 781
GluI GCNGC 1 cut(s) 98
GsaI CCCAGC 1 cut(s) 673
HaeIII GGCC 2 cut(s) 206, 651
HapII CCGG 1 cut(s) 737
Hin1II CATG 5 cut(s) 330, 355, 383, 629, 715
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 1 cut(s) 402
HpaII CCGG 1 cut(s) 737
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 5 cut(s) 64, 85, 234, 343, 385
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 5 cut(s) 295, 607, 626, 682, 730
Hpy8I GTNNAC 5 cut(s) 64, 85, 234, 343, 385
HpyAV CCTTC 5 cut(s) 8, 235, 685, 734, 760
HpyCH4III ACNGT 4 cut(s) 211, 283, 347, 553
HpyCH4V TGCA 4 cut(s) 121, 286, 343, 355
HpyF10VI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
HpyF3I CTNAG 3 cut(s) 216, 390, 496
Hsp92II CATG 5 cut(s) 330, 355, 383, 629, 715
Kzo9I GATC 2 cut(s) 258, 559
LmnI GCTCC 1 cut(s) 425
LweI GCATC 1 cut(s) 364
MaeI CTAG 3 cut(s) 314, 491, 781
MaeIII GTNAC 1 cut(s) 347
MalI GATC 2 cut(s) 260, 561
MboI GATC 2 cut(s) 258, 559
MboII GAAGA 5 cut(s) 141, 149, 157, 520, 670
MfeI CAATTG 2 cut(s) 54, 420
MhlI GDGCHC 3 cut(s) 345, 430, 481
MluCI AATT 5 cut(s) 54, 109, 264, 365, 420
MlyI GAGTC 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 357
MseI TTAA 2 cut(s) 174, 546
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 2 cut(s) 203, 582
MunI CAATTG 2 cut(s) 54, 420
MvaI CCWGG 2 cut(s) 203, 582
MwoI GCNNNNNNNGC 6 cut(s) 193, 212, 425, 515, 577, 767
NdeII GATC 2 cut(s) 258, 559
NlaIII CATG 5 cut(s) 330, 355, 383, 629, 715
NlaIV GGNNCC 4 cut(s) 43, 156, 427, 706
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 2 cut(s) 355, 715
PagI TCATGA 1 cut(s) 625
PceI AGGCCT 1 cut(s) 651
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
PpuMI RGGWCCY 1 cut(s) 42
PsiI TTATAA 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 42
Psp6I CCWGG 2 cut(s) 201, 580
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 201, 580
PspN4I GGNNCC 4 cut(s) 43, 156, 427, 706
PspPI GGNCC 1 cut(s) 42
PspPPI RGGWCCY 1 cut(s) 42
RsaI GTAC 1 cut(s) 659
RsaNI GTAC 1 cut(s) 658
RseI CAYNNNNRTG 1 cut(s) 710
SaqAI TTAA 2 cut(s) 174, 546
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 258, 559
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 203, 582
SduI GDGCHC 3 cut(s) 345, 430, 481
SetI ASST 7 cut(s) 19, 47, 181, 198, 239, 254, 710
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 5 cut(s) 54, 109, 264, 365, 420
SseBI AGGCCT 1 cut(s) 651
SsiI CCGC 4 cut(s) 77, 98, 115, 376
SspMI CTAG 3 cut(s) 314, 491, 781
StuI AGGCCT 1 cut(s) 651
StyD4I CCNGG 2 cut(s) 201, 580
TaaI ACNGT 4 cut(s) 211, 283, 347, 553
TaqI TCGA 4 cut(s) 239, 275, 462, 562
TasI AATT 5 cut(s) 54, 109, 264, 365, 420
TatI WGTACW 1 cut(s) 657
TauI GCSGC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 174, 546
Tru9I TTAA 2 cut(s) 174, 546
TscAI CASTG 1 cut(s) 622
TspDTI ATGAA 1 cut(s) 779
TspRI CASTG 1 cut(s) 622
VneI GTGCAC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 365
XceI RCATGY 2 cut(s) 355, 715
XspI CTAG 3 cut(s) 314, 491, 781
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.