MD02G1032000.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001109
Physical Location & Seq
Forward (+)
2502428 .. 2502895
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1032000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTCATTCAAAGTTTCGGATGAGTTATTGAGGATATTTGTCCCAATACTCTTATACTGGGTCTACGTTGGGGTTCTTCAATTCGCGGAATCCAGATTTTCGAAATTCAAAATTCTAATAATGAAAGAGTCAAATGACAAAAACCTCGTCTCTCGACCAACTGTAGTCGTCGTCCTGCTCCAGCAGTTCGGGCAGGCTACGATAGCAGGGCTGCTGTTCTTGTTAATAGGCCCGACCACTCGAGCATGCACCTGTTGTGGACCACCAGAAAGCGCTCATGGCGCTGTTCCGCGCAGGGTCCAATTTTTCTTTGCGATGCTTGTCTTGGATACTTTGCAGTATTTCATGCATGCACGAAAACAAGGTGCTTTACAAGTACACACTCCCGCCATCATAGGCTGGTGGCGCCATATGCATATGGAGCAATGTACAACCACCCCGTCAAGGAGCTGCTGGTGGACACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

156

Amino Acids

17.75

Weight (kDa)

9.5

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 63
AccII CGCG 2 cut(s) 86, 292
AciI CCGC 3 cut(s) 86, 290, 387
AcsI RAATTY 2 cut(s) 104, 111
AcyI GRCGYC 1 cut(s) 406
AfaI GTAC 2 cut(s) 378, 430
AfeI AGCGCT 1 cut(s) 274
AfiI CCNNNNNNNGG 1 cut(s) 444
AgsI TTSAA 3 cut(s) 10, 80, 109
AluBI AGCT 1 cut(s) 450
AluI AGCT 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 240
Aor51HI AGCGCT 1 cut(s) 274
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 211, 450
ApoI RAATTY 2 cut(s) 104, 111
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AspLEI GCGC 4 cut(s) 275, 284, 294, 408
AspS9I GGNCC 3 cut(s) 230, 260, 298
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 2 cut(s) 260, 298
BanI GGYRCC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 198, 437
BccI CCATC 1 cut(s) 398
BciVI GTATCC 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 154
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 3 cut(s) 276, 285, 409
BfuI GTATCC 1 cut(s) 322
BisI GCNGC 2 cut(s) 212, 451
BlsI GCNGC 2 cut(s) 213, 452
Bme18I GGWCC 2 cut(s) 260, 298
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 3 cut(s) 230, 260, 298
BmiI GGNNCC 2 cut(s) 299, 407
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 406
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse1I ACTGG 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 431
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 431
BseNI ACTGG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 198, 437
Bsh1236I CGCG 2 cut(s) 86, 292
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 444
BsmAI GTCTC 1 cut(s) 154
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 101
Bsp1407I TGTACA 1 cut(s) 428
BspACI CCGC 3 cut(s) 86, 290, 387
BspANI GGCC 1 cut(s) 231
BspFNI CGCG 2 cut(s) 86, 292
BspLI GGNNCC 2 cut(s) 299, 407
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 428
BsrI ACTGG 1 cut(s) 62
BssNI GRCGYC 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 163
BstACI GRCGYC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 428
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 195, 247, 351
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 2 cut(s) 86, 292
BstH2I RGCGCY 3 cut(s) 276, 285, 409
BstHHI GCGC 4 cut(s) 275, 284, 294, 408
BstMAI GTCTC 1 cut(s) 154
BstMWI GCNNNNNNNGC 7 cut(s) 190, 203, 279, 281, 405, 412, 421
BstNSI RCATGY 2 cut(s) 249, 353
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 2 cut(s) 86, 292
BstV1I GCAGC 2 cut(s) 198, 437
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 329
BtsCI GGATG 1 cut(s) 25
Cac8I GCNNGC 3 cut(s) 195, 247, 351
CfoI GCGC 4 cut(s) 275, 284, 294, 408
Cfr13I GGNCC 3 cut(s) 230, 260, 298
Csp6I GTAC 2 cut(s) 377, 429
CviAII CATG 5 cut(s) 246, 278, 346, 350, 465
CviJI RGCY 5 cut(s) 197, 211, 231, 399, 450
CviKI_1 RGCY 5 cut(s) 197, 211, 231, 399, 450
CviQI GTAC 2 cut(s) 377, 429
DinI GGCGCC 1 cut(s) 407
Eco47I GGWCC 2 cut(s) 260, 298
Eco47III AGCGCT 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 240
EcoT22I ATGCAT 2 cut(s) 351, 417
EgeI GGCGCC 1 cut(s) 407
EheI GGCGCC 1 cut(s) 407
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 5 cut(s) 249, 281, 349, 353, 468
FaqI GGGAC 1 cut(s) 26
FatI CATG 5 cut(s) 245, 277, 345, 349, 464
FauI CCCGC 1 cut(s) 394
FauNDI CATATG 2 cut(s) 411, 417
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 212, 451
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 212, 451
GlaI GCGC 4 cut(s) 274, 283, 293, 407
GluI GCNGC 2 cut(s) 212, 451
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 3 cut(s) 276, 285, 409
HaeIII GGCC 1 cut(s) 231
HhaI GCGC 4 cut(s) 275, 284, 294, 408
Hin1I GRCGYC 1 cut(s) 406
Hin1II CATG 5 cut(s) 249, 281, 349, 353, 468
Hin6I GCGC 4 cut(s) 273, 282, 292, 406
HinP1I GCGC 4 cut(s) 273, 282, 292, 406
HinfI GANTC 2 cut(s) 89, 128
Hpy166II GTNNAC 4 cut(s) 64, 260, 379, 459
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 2 cut(s) 93, 153
Hpy8I GTNNAC 4 cut(s) 64, 260, 379, 459
Hpy99I CGWCG 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 5 cut(s) 249, 337, 349, 353, 415
HpyF10VI GCNNNNNNNGC 7 cut(s) 190, 203, 279, 281, 405, 412, 421
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 406
Hsp92II CATG 5 cut(s) 249, 281, 349, 353, 468
HspAI GCGC 4 cut(s) 273, 282, 292, 406
KasI GGCGCC 1 cut(s) 405
LmnI GCTCC 3 cut(s) 183, 421, 447
Lsp1109I GCAGC 2 cut(s) 198, 437
LweI GCATC 1 cut(s) 306
MaeII ACGT 1 cut(s) 66
MboII GAAGA 1 cut(s) 68
MluCI AATT 4 cut(s) 80, 104, 111, 302
Mly113I GGCGCC 1 cut(s) 406
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 2 cut(s) 24, 155
Mph1103I ATGCAT 2 cut(s) 351, 417
MseI TTAA 1 cut(s) 224
MvnI CGCG 2 cut(s) 86, 292
MwoI GCNNNNNNNGC 7 cut(s) 190, 203, 279, 281, 405, 412, 421
NarI GGCGCC 1 cut(s) 406
NdeI CATATG 2 cut(s) 411, 417
NlaIII CATG 5 cut(s) 249, 281, 349, 353, 468
NlaIV GGNNCC 2 cut(s) 299, 407
NsiI ATGCAT 2 cut(s) 351, 417
NspI RCATGY 2 cut(s) 249, 353
NspV TTCGAA 1 cut(s) 101
PaeI GCATGC 2 cut(s) 249, 353
PaeR7I CTCGAG 1 cut(s) 240
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 213, 452
PleI GAGTC 1 cut(s) 136
PluTI GGCGCC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 136
PspN4I GGNNCC 2 cut(s) 299, 407
PspPI GGNCC 3 cut(s) 230, 260, 298
PspXI VCTCGAGB 1 cut(s) 240
RsaI GTAC 2 cut(s) 378, 430
RsaNI GTAC 2 cut(s) 377, 429
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 2 cut(s) 212, 451
Sau96I GGNCC 3 cut(s) 230, 260, 298
SchI GAGTC 1 cut(s) 137
SetI ASST 5 cut(s) 69, 147, 254, 367, 452
SfaNI GCATC 1 cut(s) 306
SfcI CTRYAG 1 cut(s) 162
SfoI GGCGCC 1 cut(s) 407
Sfr274I CTCGAG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 2 cut(s) 260, 298
SlaI CTCGAG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SphI GCATGC 2 cut(s) 249, 353
Sse9I AATT 4 cut(s) 80, 104, 111, 302
SsiI CCGC 3 cut(s) 86, 290, 387
SspDI GGCGCC 1 cut(s) 405
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 101, 154, 241
TasI AATT 4 cut(s) 80, 104, 111, 302
TatI WGTACW 2 cut(s) 376, 428
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 2 cut(s) 211, 450
TspDTI ATGAA 2 cut(s) 137, 334
VpaK11BI GGWCC 2 cut(s) 260, 298
XapI RAATTY 2 cut(s) 104, 111
XceI RCATGY 2 cut(s) 249, 353
XhoI CTCGAG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 63
Zsp2I ATGCAT 2 cut(s) 351, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.