Prupe.8G045600_v2.0.a1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001109
Physical Location & Seq
Reverse (-)
4770961 .. 4777365
6405 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G045600.2

Sequence Viewer

Length: 867 bp
ATGGTGTTTTGGGAAGGATATGTGAGTGATGAGGCAATGGGGACCTTTGCCCCAATTTTGGTTTACTGGTTGTATGCGGGGTTATACCAGTTGTTGCCGCCTTTGGACAATTACCGCTTGCATACAAGAAGAGAAGAAGATGATAAGAACTCGGTTCCGCTTCCCTCCGTTGTTAAAGGTGTTTTGCTTCAACAGCTTGTCCAGGCCACCGTTGCTCAGGGCCTCTTCTTGGAGCTTCTGGGTTGGAACCTAATCTCAACCACCCACTTCTTTGTGGTTTGGAGGTTGACCTCAAAGGCAAATAGCTTGGGGATCACAGTTCAGCCCTCGATACCTGTCCAGATTGTGCAGATTGTTATCGCAATGCTAGTCATGGACACGTGGCAGTACTTTGTGCACCGCTACATGCATCAGAACAAGTTTTTGTACCGCCATGTTCATTCTCAGCACCACAAACTTGTTGTTCCATATGCCATTGGAGCCCTTTATAATCACCCCCTTGAGGGTCTCTTGCTCGACACTTTCGGTGGGGCCATCTCTTTTCTAGTCTCAGGGATGACGGCACGGACGGCGGTTATATTCTTCTGCTTTGCAGTGATTAAAACTGTTGATGATCACTGTGGACTTTGGTTGCCCGGTAATATCTTCCATATTTTATTCCAGAACAATACTGCTTATCATGACATACATCATCAACTTCAAGGCTTGAAGTACAACTATTCTCAGCCGTTCTTCCCTCTATGGGATAAACTTTTTGGGACCTACATGCCTTATAATCTTGTGAAGAGACCTGAAGGGGGTTTAGAAGCAAGGGCAGTGAAGGCAATGAAAGACTATTTGAATCACAACAATCTGTCAGTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

289

Amino Acids

33.11

Weight (kDa)

7.85

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 774
AciI CCGC 7 cut(s) 77, 98, 115, 158, 400, 430, 572
AclWI GGATC 1 cut(s) 320
AcuI CTGAAG 1 cut(s) 813
AcvI CACGTG 1 cut(s) 381
AfaI GTAC 3 cut(s) 389, 428, 713
AfiI CCNNNNNNNGG 6 cut(s) 58, 229, 502, 503, 742, 797
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 4 cut(s) 191, 701, 709, 841
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 3 cut(s) 196, 235, 306
AluI AGCT 3 cut(s) 196, 235, 306
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 512, 553, 781
Alw44I GTGCAC 1 cut(s) 395
AlwI GGATC 1 cut(s) 320
AoxI GGCC 3 cut(s) 204, 220, 531
ApaLI GTGCAC 1 cut(s) 395
AspS9I GGNCC 4 cut(s) 42, 220, 531, 759
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 1 cut(s) 485
AvaII GGWCC 2 cut(s) 42, 759
BaeGI GKGCMC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 484
BbrPI CACGTG 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 399
BccI CCATC 1 cut(s) 542
BceAI ACGGC 3 cut(s) 576, 585, 712
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 613
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 3 cut(s) 512, 553, 781
BfaI CTAG 2 cut(s) 368, 545
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 203, 636
Bme18I GGWCC 2 cut(s) 42, 759
BmgT120I GGNCC 4 cut(s) 42, 220, 531, 759
BmiI GGNNCC 6 cut(s) 43, 156, 248, 481, 532, 760
BmrFI CCNGG 2 cut(s) 203, 636
BmsI GCATC 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 636
BsaAI YACGTR 1 cut(s) 381
BsaBI GATNNNNATC 1 cut(s) 356
BsaI GGTCTC 2 cut(s) 512, 781
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 229, 502, 503, 742, 797
Bse1I ACTGG 2 cut(s) 71, 88
Bse3DI GCAATG 3 cut(s) 42, 369, 831
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 1 cut(s) 203
BseGI GGATG 1 cut(s) 561
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 6 cut(s) 58, 229, 502, 503, 742, 797
BseMI GCAATG 3 cut(s) 42, 369, 831
BseMII CTCAG 4 cut(s) 230, 458, 564, 737
BseNI ACTGG 2 cut(s) 71, 88
BseSI GKGCMC 1 cut(s) 399
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 3 cut(s) 206, 222, 533
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 1 cut(s) 636
BslFI GGGAC 2 cut(s) 55, 772
BslI CCNNNNNNNGG 6 cut(s) 58, 229, 502, 503, 742, 797
BsmAI GTCTC 3 cut(s) 512, 553, 781
BsmFI GGGAC 2 cut(s) 55, 772
BsnI GGCC 3 cut(s) 206, 222, 533
Bso31I GGTCTC 2 cut(s) 512, 781
Bsp1286I GDGCHC 2 cut(s) 399, 484
Bsp143I GATC 2 cut(s) 312, 613
BspACI CCGC 7 cut(s) 77, 98, 115, 158, 400, 430, 572
BspANI GGCC 3 cut(s) 206, 222, 533
BspCNI CTCAG 4 cut(s) 229, 457, 563, 736
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 6 cut(s) 43, 156, 248, 481, 532, 760
BspPI GGATC 1 cut(s) 320
BspTNI GGTCTC 2 cut(s) 512, 781
BsrDI GCAATG 3 cut(s) 42, 369, 831
BsrI ACTGG 2 cut(s) 71, 88
BssMI GATC 2 cut(s) 312, 613
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 4 cut(s) 211, 319, 607, 620
Bst6I CTCTTC 3 cut(s) 124, 230, 779
BstBAI YACGTR 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 4 cut(s) 216, 444, 550, 723
BstF5I GGATG 1 cut(s) 561
BstKTI GATC 2 cut(s) 315, 616
BstMAI GTCTC 3 cut(s) 512, 553, 781
BstMBI GATC 2 cut(s) 312, 613
BstMWI GCNNNNNNNGC 5 cut(s) 193, 212, 479, 569, 821
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 409, 769
BstSCI CCNGG 2 cut(s) 201, 634
BstSLI GKGCMC 1 cut(s) 399
BsuRI GGCC 3 cut(s) 206, 222, 533
BtsCI GGATG 1 cut(s) 561
BtsI GCAGTG 2 cut(s) 600, 822
BtsIMutI CAGTG 3 cut(s) 600, 616, 822
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 679
Cfr13I GGNCC 4 cut(s) 42, 220, 531, 759
Csp6I GTAC 3 cut(s) 388, 427, 712
CviAII CATG 5 cut(s) 373, 406, 434, 680, 766
CviQI GTAC 3 cut(s) 388, 427, 712
DdeI CTNAG 4 cut(s) 216, 444, 550, 723
DpnI GATC 2 cut(s) 314, 615
DpnII GATC 2 cut(s) 312, 613
Eam1104I CTCTTC 3 cut(s) 124, 230, 779
EarI CTCTTC 3 cut(s) 124, 230, 779
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 2 cut(s) 512, 781
Eco47I GGWCC 2 cut(s) 42, 759
Eco57I CTGAAG 1 cut(s) 813
Eco72I CACGTG 1 cut(s) 381
EcoO109I RGGNCCY 3 cut(s) 42, 220, 759
EcoRII CCWGG 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 484
FaeI CATG 5 cut(s) 376, 409, 437, 683, 769
FaqI GGGAC 2 cut(s) 55, 772
FatI CATG 5 cut(s) 372, 405, 433, 679, 765
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 2 cut(s) 368, 545
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 3 cut(s) 206, 222, 533
HapII CCGG 1 cut(s) 636
Hin1II CATG 5 cut(s) 376, 409, 437, 683, 769
HincII GTYRAC 2 cut(s) 288, 862
HindII GTYRAC 2 cut(s) 288, 862
HinfI GANTC 1 cut(s) 841
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 1 cut(s) 636
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 5 cut(s) 64, 288, 397, 623, 862
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 3 cut(s) 340, 661, 680
Hpy8I GTNNAC 5 cut(s) 64, 288, 397, 623, 862
HpyAV CCTTC 3 cut(s) 8, 788, 814
HpyCH4III ACNGT 4 cut(s) 211, 319, 607, 620
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 121, 349, 397, 409, 593
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 212, 479, 569, 821
HpyF3I CTNAG 4 cut(s) 216, 444, 550, 723
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 5 cut(s) 376, 409, 437, 683, 769
Ksp22I TGATCA 1 cut(s) 613
KspAI GTTAAC 1 cut(s) 862
Kzo9I GATC 2 cut(s) 312, 613
LmnI GCTCC 2 cut(s) 232, 479
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 368, 545
MaeII ACGT 1 cut(s) 380
MalI GATC 2 cut(s) 314, 615
MboI GATC 2 cut(s) 312, 613
MboII GAAGA 8 cut(s) 141, 146, 149, 217, 574, 637, 724, 796
MhlI GDGCHC 2 cut(s) 399, 484
MluCI AATT 2 cut(s) 54, 109
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 8 cut(s) 25, 175, 233, 276, 301, 337, 496, 747
Mph1103I ATGCAT 1 cut(s) 411
MseI TTAA 3 cut(s) 174, 600, 861
MspI CCGG 1 cut(s) 636
MspR9I CCNGG 2 cut(s) 203, 636
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 5 cut(s) 193, 212, 479, 569, 821
NciI CCSGG 1 cut(s) 636
NdeI CATATG 1 cut(s) 469
NdeII GATC 2 cut(s) 312, 613
NlaIII CATG 5 cut(s) 376, 409, 437, 683, 769
NlaIV GGNNCC 6 cut(s) 43, 156, 248, 481, 532, 760
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 2 cut(s) 409, 769
PagI TCATGA 1 cut(s) 679
PfeI GAWTC 1 cut(s) 841
PkrI GCNGC 1 cut(s) 99
PmaCI CACGTG 1 cut(s) 381
PmlI CACGTG 1 cut(s) 381
Ppu21I YACGTR 1 cut(s) 381
PpuMI RGGWCCY 2 cut(s) 42, 759
PsiI TTATAA 2 cut(s) 489, 774
Psp5II RGGWCCY 2 cut(s) 42, 759
Psp6I CCWGG 1 cut(s) 201
PspCI CACGTG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 6 cut(s) 43, 156, 248, 481, 532, 760
PspPI GGNCC 4 cut(s) 42, 220, 531, 759
PspPPI RGGWCCY 2 cut(s) 42, 759
RsaI GTAC 3 cut(s) 389, 428, 713
RsaNI GTAC 3 cut(s) 388, 427, 712
SaqAI TTAA 3 cut(s) 174, 600, 861
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 312, 613
Sau96I GGNCC 4 cut(s) 42, 220, 531, 759
ScaI AGTACT 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 203, 636
SduI GDGCHC 2 cut(s) 399, 484
SfaNI GCATC 1 cut(s) 418
SinI GGWCC 2 cut(s) 42, 759
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 2 cut(s) 54, 109
SsiI CCGC 7 cut(s) 77, 98, 115, 158, 400, 430, 572
SspMI CTAG 2 cut(s) 368, 545
StyD4I CCNGG 2 cut(s) 201, 634
TaaI ACNGT 4 cut(s) 211, 319, 607, 620
TaiI ACGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 329, 516
TasI AATT 2 cut(s) 54, 109
TatI WGTACW 2 cut(s) 387, 711
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 1 cut(s) 841
Tru1I TTAA 3 cut(s) 174, 600, 861
Tru9I TTAA 3 cut(s) 174, 600, 861
TscAI CASTG 3 cut(s) 600, 623, 822
TspDTI ATGAA 2 cut(s) 428, 842
TspGWI ACGGA 2 cut(s) 157, 580
TspRI CASTG 3 cut(s) 600, 623, 822
VneI GTGCAC 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 42, 759
XceI RCATGY 2 cut(s) 409, 769
XspI CTAG 2 cut(s) 368, 545
ZrmI AGTACT 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.