Rmu_sc0001030.1_g000021

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001030.1
OG0001109
Physical Location & Seq
Forward (+)
77330 .. 78360
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001030.1_g000021.1.cds

Sequence Viewer

Length: 819 bp
atgagaatagttgcttctgatgaagtattaggaacgtttatccctatcatagtgtactggctgtacgcagccctcattgtatttatggaaaaatcatttccgaaatacaaaattcgaagcgcagaggagcaagacaagagaaaccacctcgtttccaaaggaacagtcatcaaaggcgtcgtcctccaacaagtcctgcaggctctcactgccacactcgtgtatattctgacaaaccgtgacgatgatgcggctgcttctatgactacttctactacaggtcaaaatgatgatggtgatgatggaattgggtcaatgatcatgatgcttgccacaactatggtccagtttctgatagccatggtggtgatggacacgtggcagtacttcatgcataggtatatgcatgagaacaagttcctctacaagcacatccactcccagcatcacaggcttgtagctccgtatgcatatgcagcattgtacaaccacccgttggagggtcttctcgtggacactatgagcggagctgtcagcttcttgctgtcgggcatgtctccgcggacttcgattttcttcttctcatttgcaaccatcaaggcagtggacgaccactccggtgtatgtaactttccatggcacccgtttcatgttatgttcagaaacaacagcttatatcatgatgtgcaccaccagctttcagggaccaagttcaacttctctcagcccttctttgtgttttgggataggctacttggtacctaccacatgccatcatctgctgaagatgagctcaacaaggaggccaagagtaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

272

Amino Acids

31.11

Weight (kDa)

6.55

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 758
AccB1I GGYRCC 2 cut(s) 639, 758
AccB7I CCANNNNNTGG 1 cut(s) 496
AccBSI CCGCTC 1 cut(s) 525
AccII CGCG 1 cut(s) 562
AciI CCGC 4 cut(s) 251, 525, 560, 562
AclI AACGTT 1 cut(s) 35
AcsI RAATTY 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 804
AcvI CACGTG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 5 cut(s) 56, 65, 386, 485, 760
AfiI CCNNNNNNNGG 3 cut(s) 496, 499, 500
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 1 cut(s) 715
AjuI GAANNNNNNNTTGG 2 cut(s) 701, 733
AleI CACNNNNGTG 2 cut(s) 218, 618
AluBI AGCT 6 cut(s) 461, 530, 537, 672, 697, 793
AluI AGCT 6 cut(s) 461, 530, 537, 672, 697, 793
Alw21I GWGCWC 2 cut(s) 690, 795
Alw26I GTCTC 1 cut(s) 561
Alw44I GTGCAC 1 cut(s) 686
AlwNI CAGNNNCTG 1 cut(s) 352
AoxI GGCC 1 cut(s) 804
ApaLI GTGCAC 1 cut(s) 686
ApeKI GCWGC 3 cut(s) 68, 254, 476
ApoI RAATTY 1 cut(s) 111
ArsI GACNNNNNNTTYG 4 cut(s) 150, 165, 182, 197
Asp700I GAANNNNTTC 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 758
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 343, 705
AsuHPI GGTGA 2 cut(s) 308, 379
AsuII TTCGAA 1 cut(s) 115
AvaII GGWCC 2 cut(s) 343, 705
BaeGI GKGCMC 1 cut(s) 690
BanI GGYRCC 2 cut(s) 639, 758
BanII GRGCYC 1 cut(s) 795
BauI CACGAG 2 cut(s) 218, 509
BbrPI CACGTG 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 2 cut(s) 690, 795
BbvI GCAGC 3 cut(s) 80, 241, 488
BccI CCATC 5 cut(s) 287, 296, 364, 602, 781
BclI TGATCA 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 197, 276
BisI GCNGC 4 cut(s) 69, 252, 255, 477
BlsI GCNGC 4 cut(s) 70, 253, 256, 478
BmcAI AGTACT 1 cut(s) 386
Bme18I GGWCC 2 cut(s) 343, 705
BmgT120I GGNCC 2 cut(s) 343, 705
BmiI GGNNCC 3 cut(s) 641, 706, 760
BmsI GCATC 3 cut(s) 238, 315, 454
BpiI GAAGAC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 115
BsaAI YACGTR 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 360, 560, 635
BsaWI WCCGGW 1 cut(s) 617
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 599, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 496, 499, 500
Bse1I ACTGG 2 cut(s) 62, 346
BseDI CCNNGG 3 cut(s) 360, 560, 635
BseGI GGATG 1 cut(s) 432
BseLI CCNNNNNNNGG 3 cut(s) 496, 499, 500
BseMII CTCAG 1 cut(s) 737
BseNI ACTGG 2 cut(s) 62, 346
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 1 cut(s) 690
BseXI GCAGC 3 cut(s) 80, 241, 488
BseYI CCCAGC 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 1 cut(s) 806
BshNI GGYRCC 2 cut(s) 639, 758
BsiHKAI GWGCWC 2 cut(s) 690, 795
BsiSI CCGG 1 cut(s) 618
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 3 cut(s) 496, 499, 500
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 718
BsnI GGCC 1 cut(s) 806
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 2 cut(s) 690, 795
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 1 cut(s) 318
Bsp19I CCATGG 2 cut(s) 360, 635
BspACI CCGC 4 cut(s) 251, 525, 560, 562
BspANI GGCC 1 cut(s) 806
BspCNI CTCAG 1 cut(s) 736
BspFNI CGCG 1 cut(s) 562
BspHI TCATGA 2 cut(s) 321, 679
BspLI GGNNCC 3 cut(s) 641, 706, 760
BspMAI CTGCAG 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 115
BspT107I GGYRCC 2 cut(s) 639, 758
BsrBI CCGCTC 1 cut(s) 525
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 2 cut(s) 62, 346
BssECI CCNNGG 3 cut(s) 360, 560, 635
BssMI GATC 1 cut(s) 318
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 2 cut(s) 218, 509
BssT1I CCWWGG 2 cut(s) 360, 635
Bst2BI CACGAG 2 cut(s) 218, 509
Bst4CI ACNGT 2 cut(s) 166, 239
BstACI GRCGYC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 201, 330
BstDEI CTNAG 1 cut(s) 723
BstDSI CCRYGG 3 cut(s) 360, 560, 635
BstF5I GGATG 1 cut(s) 432
BstFNI CGCG 1 cut(s) 562
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 5 cut(s) 209, 451, 467, 476, 694
BstNSI RCATGY 2 cut(s) 556, 772
BstSFI CTRYAG 2 cut(s) 197, 276
BstSLI GKGCMC 1 cut(s) 690
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 3 cut(s) 80, 241, 488
BstV2I GAAGAC 1 cut(s) 497
BstXI CCANNNNNNTGG 1 cut(s) 340
BsuRI GGCC 1 cut(s) 806
BtgI CCRYGG 3 cut(s) 360, 560, 635
BtsCI GGATG 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 207, 609
BtsIMutI CAGTG 2 cut(s) 207, 609
Cac8I GCNNGC 2 cut(s) 201, 330
CaiI CAGNNNCTG 1 cut(s) 352
CciI TCATGA 2 cut(s) 321, 679
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 343, 705
Cfr42I CCGCGG 1 cut(s) 563
CseI GACGC 1 cut(s) 166
Csp6I GTAC 5 cut(s) 55, 64, 385, 484, 759
CviAII CATG 9 cut(s) 322, 361, 391, 407, 553, 636, 650, 680, 769
CviQI GTAC 5 cut(s) 55, 64, 385, 484, 759
DdeI CTNAG 1 cut(s) 723
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 793
Eco130I CCWWGG 2 cut(s) 360, 635
Eco24I GRGCYC 1 cut(s) 795
Eco47I GGWCC 2 cut(s) 343, 705
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 1 cut(s) 804
Eco72I CACGTG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 793
EcoT14I CCWWGG 2 cut(s) 360, 635
EcoT22I ATGCAT 3 cut(s) 396, 408, 472
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 2 cut(s) 360, 635
FaeI CATG 9 cut(s) 325, 364, 394, 410, 556, 639, 653, 683, 772
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 1 cut(s) 718
FatI CATG 9 cut(s) 321, 360, 390, 406, 552, 635, 649, 679, 768
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 318
Fnu4HI GCNGC 4 cut(s) 69, 252, 255, 477
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 4 cut(s) 69, 252, 255, 477
GlaI GCGC 1 cut(s) 121
GluI GCNGC 4 cut(s) 69, 252, 255, 477
GsaI CCCAGC 1 cut(s) 445
HaeIII GGCC 1 cut(s) 806
HapII CCGG 1 cut(s) 618
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 9 cut(s) 325, 364, 394, 410, 556, 639, 653, 683, 772
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HpaII CCGG 1 cut(s) 618
HphI GGTGA 2 cut(s) 308, 379
Hpy166II GTNNAC 4 cut(s) 55, 514, 607, 688
Hpy188I TCNGA 5 cut(s) 19, 102, 231, 354, 662
Hpy188III TCNNGA 2 cut(s) 322, 680
Hpy8I GTNNAC 4 cut(s) 55, 514, 607, 688
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 739
HpyCH4III ACNGT 2 cut(s) 166, 239
HpyCH4IV ACGT 2 cut(s) 35, 377
HpyCH4V TGCA 7 cut(s) 199, 394, 406, 470, 476, 590, 688
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 451, 467, 476, 694
HpyF3I CTNAG 1 cut(s) 723
HpySE526I ACGT 2 cut(s) 35, 377
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 9 cut(s) 325, 364, 394, 410, 556, 639, 653, 683, 772
HspAI GCGC 1 cut(s) 120
KpnI GGTACC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 318
KspI CCGCGG 1 cut(s) 563
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 3 cut(s) 127, 466, 527
Lsp1109I GCAGC 3 cut(s) 80, 241, 488
LweI GCATC 3 cut(s) 238, 315, 454
MaeII ACGT 2 cut(s) 35, 377
MaeIII GTNAC 2 cut(s) 239, 626
MalI GATC 1 cut(s) 320
MbiI CCGCTC 1 cut(s) 525
MboI GATC 1 cut(s) 318
MboII GAAGA 4 cut(s) 497, 568, 571, 797
MhlI GDGCHC 2 cut(s) 690, 795
MluCI AATT 2 cut(s) 111, 306
MmeI TCCRAC 2 cut(s) 211, 477
MnlI CCTC 7 cut(s) 83, 118, 158, 194, 431, 493, 796
Mph1103I ATGCAT 3 cut(s) 396, 408, 472
MroXI GAANNNNTTC 1 cut(s) 416
MslI CAYNNNNRTG 4 cut(s) 218, 338, 365, 618
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 1 cut(s) 618
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 5 cut(s) 209, 451, 467, 476, 694
NcoI CCATGG 2 cut(s) 360, 635
NdeI CATATG 1 cut(s) 472
NdeII GATC 1 cut(s) 318
NlaIII CATG 9 cut(s) 325, 364, 394, 410, 556, 639, 653, 683, 772
NlaIV GGNNCC 3 cut(s) 641, 706, 760
NmuCI GTSAC 1 cut(s) 239
NsiI ATGCAT 3 cut(s) 396, 408, 472
NspI RCATGY 2 cut(s) 556, 772
NspV TTCGAA 1 cut(s) 115
OliI CACNNNNGTG 2 cut(s) 218, 618
PagI TCATGA 2 cut(s) 321, 679
PdmI GAANNNNTTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 496
PkrI GCNGC 4 cut(s) 70, 253, 256, 478
PmaCI CACGTG 1 cut(s) 378
PmlI CACGTG 1 cut(s) 378
Ppu21I YACGTR 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 795
Psp1406I AACGTT 1 cut(s) 35
PspCI CACGTG 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 441
PspN4I GGNNCC 3 cut(s) 641, 706, 760
PspPI GGNCC 2 cut(s) 343, 705
PstI CTGCAG 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 352
RsaI GTAC 5 cut(s) 56, 65, 386, 485, 760
RsaNI GTAC 5 cut(s) 55, 64, 385, 484, 759
RseI CAYNNNNRTG 4 cut(s) 218, 338, 365, 618
SacI GAGCTC 1 cut(s) 795
SacII CCGCGG 1 cut(s) 563
SatI GCNGC 4 cut(s) 69, 252, 255, 477
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 2 cut(s) 343, 705
SbfI CCTGCAGG 1 cut(s) 201
ScaI AGTACT 1 cut(s) 386
SdaI CCTGCAGG 1 cut(s) 201
SduI GDGCHC 2 cut(s) 690, 795
SfaNI GCATC 3 cut(s) 238, 315, 454
SfcI CTRYAG 2 cut(s) 197, 276
Sfr303I CCGCGG 1 cut(s) 563
SfuI TTCGAA 1 cut(s) 115
SgrBI CCGCGG 1 cut(s) 563
SinI GGWCC 2 cut(s) 343, 705
SmiMI CAYNNNNRTG 4 cut(s) 218, 338, 365, 618
Sse8387I CCTGCAGG 1 cut(s) 201
Sse9I AATT 2 cut(s) 111, 306
SsiI CCGC 4 cut(s) 251, 525, 560, 562
SstI GAGCTC 1 cut(s) 795
StyI CCWWGG 2 cut(s) 360, 635
TaaI ACNGT 2 cut(s) 166, 239
TaiI ACGT 2 cut(s) 38, 380
TaqI TCGA 2 cut(s) 115, 569
TasI AATT 2 cut(s) 111, 306
TatI WGTACW 3 cut(s) 54, 384, 483
TauI GCSGC 1 cut(s) 254
TscAI CASTG 2 cut(s) 214, 609
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 3 cut(s) 68, 254, 476
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 3 cut(s) 36, 379, 638
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 214, 609
Van91I CCANNNNNTGG 1 cut(s) 496
VneI GTGCAC 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 343, 705
XapI RAATTY 1 cut(s) 111
XceI RCATGY 2 cut(s) 556, 772
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 601
XmnI GAANNNNTTC 1 cut(s) 416
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 3 cut(s) 396, 408, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.