RchiOBHm_Chr7g0201821

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001109
Physical Location & Seq
Forward (+)
19448532 .. 19449571
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18065

Sequence Viewer

Length: 828 bp
ATGGGATTCATGAGAATAGTTGCTTCTGATGAAGTATTAGGAACGTTTATCCCTATCATAGTGTACTGGCTGTACGCAGCCCTCATTGTATTTATGGAAAAATCATTTCCGAAATACAAAATTCGAAGCGCAGAGGAGCAAGACAAGAGAAACCACCTCGTTTCCAAAGGAACAGTCATCAAAGGCGTCGTCCTCCAACAAGTCCTGCAGGCTCTCACTGCCACACTCGTGTATATTCTGACAAACCGTGACGATGATGCGGCTGCTTCTATGACTACTTCTACTACAGGTCAAAATGATGATGGTGATGATGGAATTGGGTCAATGATCATGATGCTTGCCACAACTATGGTCCAGTTTCTGATAGCCATGGTGGTGATGGACACGTGGCAGTACTTCATGCATAGGTATATGCATGAGAACAAGTTCCTCTACAAGCACATCCACTCCCAGCATCACAGGCTTGTAGCTCCGTATGCATATGCAGCATTGTACAACCACCCGTTGGAGGGTCTTCTCGTGGACACTATGAGCGGAGCTGTCAGCTTCTTGCTGTCGGGCATGTCTCCGCGGACTTCGATTTTCTTCTTCTCATTTGCAACCATCAAGGCAGTGGACGACCACTCCGGTGTATGTAACTTTCCATGGCACCCGTTTCATGTTATGTTCAGAAACAACAGCTTATATCATGATGTGCACCACCAGCTTTCAGGGACCAAGTTCAACTTCTCTCAGCCCTTCTTTGTGTTTTGGGATAGGCTACTTGGTACCTACCACATGCCATCATCTGCTGAAGATGAGCTCAACAAGGAGGCCAAGAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

275

Amino Acids

31.44

Weight (kDa)

6.55

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 120 - 257 3.7e-26 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 2 cut(s) 648, 767
AccB7I CCANNNNNTGG 1 cut(s) 505
AccBSI CCGCTC 1 cut(s) 534
AccII CGCG 1 cut(s) 571
AciI CCGC 4 cut(s) 260, 534, 569, 571
AclI AACGTT 1 cut(s) 44
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 813
AcvI CACGTG 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 5 cut(s) 65, 74, 395, 494, 769
AfiI CCNNNNNNNGG 3 cut(s) 505, 508, 509
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 1 cut(s) 724
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AleI CACNNNNGTG 2 cut(s) 227, 627
AluBI AGCT 6 cut(s) 470, 539, 546, 681, 706, 802
AluI AGCT 6 cut(s) 470, 539, 546, 681, 706, 802
Alw21I GWGCWC 2 cut(s) 699, 804
Alw26I GTCTC 1 cut(s) 570
Alw44I GTGCAC 1 cut(s) 695
AlwNI CAGNNNCTG 1 cut(s) 361
AoxI GGCC 1 cut(s) 813
ApaLI GTGCAC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 77, 263, 485
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 4 cut(s) 159, 174, 191, 206
Asp700I GAANNNNTTC 1 cut(s) 425
Asp718I GGTACC 1 cut(s) 767
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 352, 714
AsuHPI GGTGA 2 cut(s) 317, 388
AsuII TTCGAA 1 cut(s) 124
AvaII GGWCC 2 cut(s) 352, 714
BaeGI GKGCMC 1 cut(s) 699
BanI GGYRCC 2 cut(s) 648, 767
BanII GRGCYC 1 cut(s) 804
BauI CACGAG 2 cut(s) 227, 518
BbrPI CACGTG 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 2 cut(s) 699, 804
BbvI GCAGC 3 cut(s) 89, 250, 497
BccI CCATC 5 cut(s) 296, 305, 373, 611, 790
BclI TGATCA 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 570
BfmI CTRYAG 2 cut(s) 206, 285
BisI GCNGC 4 cut(s) 78, 261, 264, 486
BlsI GCNGC 4 cut(s) 79, 262, 265, 487
BmcAI AGTACT 1 cut(s) 395
Bme18I GGWCC 2 cut(s) 352, 714
BmgT120I GGNCC 2 cut(s) 352, 714
BmiI GGNNCC 3 cut(s) 650, 715, 769
BmsI GCATC 3 cut(s) 247, 324, 463
BpiI GAAGAC 1 cut(s) 506
Bpu14I TTCGAA 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 369, 569, 644
BsaWI WCCGGW 1 cut(s) 626
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 608, 638
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 508, 509
Bse1I ACTGG 2 cut(s) 71, 355
BseDI CCNNGG 3 cut(s) 369, 569, 644
BseGI GGATG 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 505, 508, 509
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 2 cut(s) 71, 355
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 699
BseXI GCAGC 3 cut(s) 89, 250, 497
BseYI CCCAGC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 571
BshFI GGCC 1 cut(s) 815
BshNI GGYRCC 2 cut(s) 648, 767
BsiHKAI GWGCWC 2 cut(s) 699, 804
BsiSI CCGG 1 cut(s) 627
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 3 cut(s) 505, 508, 509
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 699, 804
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 327
Bsp19I CCATGG 2 cut(s) 369, 644
BspACI CCGC 4 cut(s) 260, 534, 569, 571
BspANI GGCC 1 cut(s) 815
BspCNI CTCAG 1 cut(s) 745
BspFNI CGCG 1 cut(s) 571
BspHI TCATGA 3 cut(s) 9, 330, 688
BspLI GGNNCC 3 cut(s) 650, 715, 769
BspMAI CTGCAG 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 124
BspT107I GGYRCC 2 cut(s) 648, 767
BsrBI CCGCTC 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 71, 355
BssECI CCNNGG 3 cut(s) 369, 569, 644
BssMI GATC 1 cut(s) 327
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 2 cut(s) 227, 518
BssT1I CCWWGG 2 cut(s) 369, 644
Bst2BI CACGAG 2 cut(s) 227, 518
Bst4CI ACNGT 2 cut(s) 175, 248
BstACI GRCGYC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 492
BstBAI YACGTR 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 210, 339
BstDEI CTNAG 1 cut(s) 732
BstDSI CCRYGG 3 cut(s) 369, 569, 644
BstF5I GGATG 1 cut(s) 441
BstFNI CGCG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 1 cut(s) 330
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 1 cut(s) 327
BstMWI GCNNNNNNNGC 5 cut(s) 218, 460, 476, 485, 703
BstNSI RCATGY 2 cut(s) 565, 781
BstSFI CTRYAG 2 cut(s) 206, 285
BstSLI GKGCMC 1 cut(s) 699
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 3 cut(s) 89, 250, 497
BstV2I GAAGAC 1 cut(s) 506
BstXI CCANNNNNNTGG 1 cut(s) 349
BsuRI GGCC 1 cut(s) 815
BtgI CCRYGG 3 cut(s) 369, 569, 644
BtsCI GGATG 1 cut(s) 441
BtsI GCAGTG 2 cut(s) 216, 618
BtsIMutI CAGTG 2 cut(s) 216, 618
Cac8I GCNNGC 2 cut(s) 210, 339
CaiI CAGNNNCTG 1 cut(s) 361
CciI TCATGA 3 cut(s) 9, 330, 688
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 352, 714
Cfr42I CCGCGG 1 cut(s) 572
CseI GACGC 1 cut(s) 175
Csp6I GTAC 5 cut(s) 64, 73, 394, 493, 768
CviQI GTAC 5 cut(s) 64, 73, 394, 493, 768
DdeI CTNAG 1 cut(s) 732
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 802
Eco130I CCWWGG 2 cut(s) 369, 644
Eco24I GRGCYC 1 cut(s) 804
Eco47I GGWCC 2 cut(s) 352, 714
Eco53kI GAGCTC 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 813
Eco72I CACGTG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 369, 644
EcoT22I ATGCAT 3 cut(s) 405, 417, 481
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 2 cut(s) 369, 644
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 727
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 4 cut(s) 78, 261, 264, 486
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 4 cut(s) 78, 261, 264, 486
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 78, 261, 264, 486
GsaI CCCAGC 1 cut(s) 454
HaeIII GGCC 1 cut(s) 815
HapII CCGG 1 cut(s) 627
HgaI GACGC 1 cut(s) 175
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 1 cut(s) 6
HpaII CCGG 1 cut(s) 627
HphI GGTGA 2 cut(s) 317, 388
Hpy166II GTNNAC 4 cut(s) 64, 523, 616, 697
Hpy188I TCNGA 5 cut(s) 28, 111, 240, 363, 671
Hpy188III TCNNGA 3 cut(s) 10, 331, 689
Hpy8I GTNNAC 4 cut(s) 64, 523, 616, 697
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 748
HpyCH4III ACNGT 2 cut(s) 175, 248
HpyCH4IV ACGT 2 cut(s) 44, 386
HpyCH4V TGCA 7 cut(s) 208, 403, 415, 479, 485, 599, 697
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 460, 476, 485, 703
HpyF3I CTNAG 1 cut(s) 732
HpySE526I ACGT 2 cut(s) 44, 386
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 771
Ksp22I TGATCA 1 cut(s) 327
KspI CCGCGG 1 cut(s) 572
Kzo9I GATC 1 cut(s) 327
LmnI GCTCC 3 cut(s) 136, 475, 536
Lsp1109I GCAGC 3 cut(s) 89, 250, 497
LweI GCATC 3 cut(s) 247, 324, 463
MaeII ACGT 2 cut(s) 44, 386
MaeIII GTNAC 2 cut(s) 248, 635
MalI GATC 1 cut(s) 329
MbiI CCGCTC 1 cut(s) 534
MboI GATC 1 cut(s) 327
MboII GAAGA 4 cut(s) 506, 577, 580, 806
MhlI GDGCHC 2 cut(s) 699, 804
MluCI AATT 2 cut(s) 120, 315
MmeI TCCRAC 2 cut(s) 220, 486
MnlI CCTC 7 cut(s) 92, 127, 167, 203, 440, 502, 805
Mph1103I ATGCAT 3 cut(s) 405, 417, 481
MroXI GAANNNNTTC 1 cut(s) 425
MslI CAYNNNNRTG 4 cut(s) 227, 347, 374, 627
MspA1I CMGCKG 1 cut(s) 571
MspI CCGG 1 cut(s) 627
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 5 cut(s) 218, 460, 476, 485, 703
NcoI CCATGG 2 cut(s) 369, 644
NdeI CATATG 1 cut(s) 481
NdeII GATC 1 cut(s) 327
NlaIV GGNNCC 3 cut(s) 650, 715, 769
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 3 cut(s) 405, 417, 481
NspI RCATGY 2 cut(s) 565, 781
NspV TTCGAA 1 cut(s) 124
OliI CACNNNNGTG 2 cut(s) 227, 627
PagI TCATGA 3 cut(s) 9, 330, 688
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 4 cut(s) 79, 262, 265, 487
PmaCI CACGTG 1 cut(s) 387
PmlI CACGTG 1 cut(s) 387
Ppu21I YACGTR 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 804
Psp1406I AACGTT 1 cut(s) 44
PspCI CACGTG 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 650, 715, 769
PspPI GGNCC 2 cut(s) 352, 714
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 361
RsaI GTAC 5 cut(s) 65, 74, 395, 494, 769
RsaNI GTAC 5 cut(s) 64, 73, 394, 493, 768
RseI CAYNNNNRTG 4 cut(s) 227, 347, 374, 627
SacI GAGCTC 1 cut(s) 804
SacII CCGCGG 1 cut(s) 572
SatI GCNGC 4 cut(s) 78, 261, 264, 486
Sau3AI GATC 1 cut(s) 327
Sau96I GGNCC 2 cut(s) 352, 714
SbfI CCTGCAGG 1 cut(s) 210
ScaI AGTACT 1 cut(s) 395
SdaI CCTGCAGG 1 cut(s) 210
SduI GDGCHC 2 cut(s) 699, 804
SfaNI GCATC 3 cut(s) 247, 324, 463
SfcI CTRYAG 2 cut(s) 206, 285
Sfr303I CCGCGG 1 cut(s) 572
SfuI TTCGAA 1 cut(s) 124
SgrBI CCGCGG 1 cut(s) 572
SinI GGWCC 2 cut(s) 352, 714
SmiMI CAYNNNNRTG 4 cut(s) 227, 347, 374, 627
Sse8387I CCTGCAGG 1 cut(s) 210
Sse9I AATT 2 cut(s) 120, 315
SsiI CCGC 4 cut(s) 260, 534, 569, 571
SstI GAGCTC 1 cut(s) 804
StyI CCWWGG 2 cut(s) 369, 644
TaaI ACNGT 2 cut(s) 175, 248
TaiI ACGT 2 cut(s) 47, 389
TaqI TCGA 2 cut(s) 124, 578
TasI AATT 2 cut(s) 120, 315
TatI WGTACW 3 cut(s) 63, 393, 492
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 6
TscAI CASTG 2 cut(s) 223, 618
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 3 cut(s) 77, 263, 485
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 45, 388, 647
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 2 cut(s) 223, 618
Van91I CCANNNNNTGG 1 cut(s) 505
VneI GTGCAC 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 352, 714
XapI RAATTY 1 cut(s) 120
XceI RCATGY 2 cut(s) 565, 781
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 610
XmnI GAANNNNTTC 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 395
Zsp2I ATGCAT 3 cut(s) 405, 417, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.