RLG00000002928

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001109
Physical Location & Seq
Forward (+)
41859506 .. 41860589
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002928

Sequence Viewer

Length: 714 bp
ATGAAGGTTTGGGTTTGGGTTGGGGTAGAGAAGAGAAAAGAAAGGAAGAAGAGGGTTTTGGCGGTAGTTGTAGTTGGTGCTGGTGGGGTTTTTGATCTTCTTCTTCGTTGGAAGGGGCGTAGTTTGCTAGCAGAGTCCATCAGAGCGAACGAGCGTCGAGATGGAGAAAGAGACGAGAGTTCAGCGAGTCAAAATAATTGTGGGTTAATGATCATGACGCTTGTCACAACTACGGTCCAGTTTCTGATAGCTATGGTGGTCATGGACACATGGCAGTACTTCATGCACAGATATATGCATGAGAACAAGTTTGTTTACAAGCACGTCCACTCCCAGCATCACAGGCTGGTGGCTCCGTATGCATATGGTGCACTGTACAACCACCCGTTGGAGGGTCTTCTCGTGGATACCATGGCGGGAGCCGTCAGCTTCTTGGTCTCTGGGATGTCTCCGCGGACTTCGATTTTCTTCTTCTCCTTTGCAACCATCAAAGCAGTGGACGACCACTCCGGCCTATTTAACTTTCCATGGCACCCGTTTCACGTCTTATTCGAAAACAATAGCCTGTATCATGATTTGCACCATCAGCCTTCTGGGATAAAGTATAACTACTCCCAGCCATTCTTCGTTTTTTGGGATAAGATACTCGGTACCTATCACATGCCAATGATCCCACCATCTTATGAAGACGCCATATGGACCGTCACGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

238

Amino Acids

27.52

Weight (kDa)

9.08

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 81 - 218 2.3e-25 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 2 cut(s) 531, 650
AccB7I CCANNNNNTGG 1 cut(s) 388
AccII CGCG 1 cut(s) 454
AciI CCGC 4 cut(s) 62, 416, 452, 454
AclWI GGATC 1 cut(s) 664
AcyI GRCGYC 1 cut(s) 690
AfaI GTAC 3 cut(s) 278, 377, 652
AfiI CCNNNNNNNGG 3 cut(s) 388, 391, 392
AjiI CACGTC 2 cut(s) 325, 544
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 2 cut(s) 251, 429
AluI AGCT 2 cut(s) 251, 429
Alw21I GWGCWC 1 cut(s) 373
Alw26I GTCTC 3 cut(s) 165, 442, 453
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 1 cut(s) 664
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 1 cut(s) 511
ApaLI GTGCAC 1 cut(s) 369
Asp718I GGTACC 1 cut(s) 650
AspS9I GGNCC 2 cut(s) 235, 699
AsuII TTCGAA 1 cut(s) 552
AsuNHI GCTAGC 1 cut(s) 127
AvaII GGWCC 2 cut(s) 235, 699
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 2 cut(s) 531, 650
BauI CACGAG 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 389, 693
Bbv12I GWGCWC 1 cut(s) 373
BccI CCATC 5 cut(s) 146, 155, 494, 591, 685
BceAI ACGGC 1 cut(s) 407
BciVI GTATCC 1 cut(s) 400
BclI TGATCA 1 cut(s) 210
BcoDI GTCTC 3 cut(s) 165, 442, 453
BfaI CTAG 1 cut(s) 128
BfuI GTATCC 1 cut(s) 400
BmcAI AGTACT 1 cut(s) 278
Bme18I GGWCC 2 cut(s) 235, 699
BmgBI CACGTC 2 cut(s) 325, 544
BmgT120I GGNCC 2 cut(s) 235, 699
BmiI GGNNCC 4 cut(s) 354, 421, 533, 652
BmsI GCATC 1 cut(s) 346
BmtI GCTAGC 1 cut(s) 131
BoxI GACNNNNGTC 1 cut(s) 221
BpiI GAAGAC 2 cut(s) 389, 693
Bpu14I TTCGAA 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 442
BsaJI CCNNGG 3 cut(s) 411, 452, 527
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 491, 521
Bsc4I CCNNNNNNNGG 3 cut(s) 388, 391, 392
Bse1I ACTGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 411, 452, 527
BseGI GGATG 1 cut(s) 450
BseLI CCNNNNNNNGG 3 cut(s) 388, 391, 392
BseNI ACTGG 1 cut(s) 238
BseSI GKGCMC 1 cut(s) 373
BseYI CCCAGC 2 cut(s) 333, 615
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 1 cut(s) 513
BshNI GGYRCC 2 cut(s) 531, 650
BsiHKAI GWGCWC 1 cut(s) 373
BsiSI CCGG 1 cut(s) 510
BslI CCNNNNNNNGG 3 cut(s) 388, 391, 392
BsmAI GTCTC 3 cut(s) 165, 442, 453
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 1 cut(s) 513
Bso31I GGTCTC 1 cut(s) 442
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 373
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 94, 210, 669
Bsp19I CCATGG 2 cut(s) 411, 527
BspACI CCGC 4 cut(s) 62, 416, 452, 454
BspANI GGCC 1 cut(s) 513
BspFNI CGCG 1 cut(s) 454
BspHI TCATGA 2 cut(s) 213, 571
BspLI GGNNCC 4 cut(s) 354, 421, 533, 652
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 664
BspT104I TTCGAA 1 cut(s) 552
BspT107I GGYRCC 2 cut(s) 531, 650
BspTNI GGTCTC 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 411, 452, 527
BssMI GATC 3 cut(s) 94, 210, 669
BssNI GRCGYC 1 cut(s) 690
BssSI CACGAG 1 cut(s) 401
BssT1I CCWWGG 2 cut(s) 411, 527
Bst2BI CACGAG 1 cut(s) 401
Bst4CI ACNGT 3 cut(s) 235, 375, 703
Bst6I CTCTTC 2 cut(s) 26, 44
BstACI GRCGYC 1 cut(s) 690
BstAPI GCANNNNNTGC 1 cut(s) 368
BstAUI TGTACA 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 1 cut(s) 129
BstDSI CCRYGG 3 cut(s) 411, 452, 527
BstF5I GGATG 1 cut(s) 450
BstFNI CGCG 1 cut(s) 454
BstKTI GATC 3 cut(s) 97, 213, 672
BstMAI GTCTC 3 cut(s) 165, 442, 453
BstMBI GATC 3 cut(s) 94, 210, 669
BstMWI GCNNNNNNNGC 5 cut(s) 124, 343, 359, 368, 586
BstNSI RCATGY 1 cut(s) 664
BstPAI GACNNNNGTC 1 cut(s) 221
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 454
BstV2I GAAGAC 2 cut(s) 389, 693
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 3 cut(s) 411, 452, 527
BtrI CACGTC 2 cut(s) 325, 544
BtsCI GGATG 1 cut(s) 450
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 2 cut(s) 371, 501
Cac8I GCNNGC 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 244
CciI TCATGA 2 cut(s) 213, 571
Cfr13I GGNCC 2 cut(s) 235, 699
Cfr42I CCGCGG 1 cut(s) 455
CseI GACGC 3 cut(s) 143, 226, 698
Csp6I GTAC 3 cut(s) 277, 376, 651
CviAII CATG 9 cut(s) 214, 262, 270, 283, 299, 412, 528, 572, 661
CviJI RGCY 9 cut(s) 251, 346, 353, 422, 429, 513, 564, 589, 619
CviKI_1 RGCY 9 cut(s) 251, 346, 353, 422, 429, 513, 564, 589, 619
CviQI GTAC 3 cut(s) 277, 376, 651
DpnI GATC 3 cut(s) 96, 212, 671
DpnII GATC 3 cut(s) 94, 210, 669
Eam1104I CTCTTC 2 cut(s) 26, 44
EarI CTCTTC 2 cut(s) 26, 44
Eco130I CCWWGG 2 cut(s) 411, 527
Eco31I GGTCTC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 235, 699
EcoT14I CCWWGG 2 cut(s) 411, 527
EcoT22I ATGCAT 2 cut(s) 300, 364
ErhI CCWWGG 2 cut(s) 411, 527
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 9 cut(s) 217, 265, 273, 286, 302, 415, 531, 575, 664
FatI CATG 9 cut(s) 213, 261, 269, 282, 298, 411, 527, 571, 660
FauI CCCGC 1 cut(s) 409
FauNDI CATATG 2 cut(s) 364, 695
FbaI TGATCA 1 cut(s) 210
FokI GGATG 1 cut(s) 457
FspBI CTAG 1 cut(s) 128
GsaI CCCAGC 2 cut(s) 337, 619
HaeIII GGCC 1 cut(s) 513
HapII CCGG 1 cut(s) 510
HgaI GACGC 3 cut(s) 143, 226, 698
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 9 cut(s) 217, 265, 273, 286, 302, 415, 531, 575, 664
HinfI GANTC 2 cut(s) 134, 187
HpaII CCGG 1 cut(s) 510
Hpy166II GTNNAC 4 cut(s) 316, 328, 371, 499
Hpy188I TCNGA 2 cut(s) 143, 246
Hpy188III TCNNGA 4 cut(s) 158, 214, 572, 706
Hpy8I GTNNAC 4 cut(s) 316, 328, 371, 499
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 2 cut(s) 106, 600
HpyCH4III ACNGT 3 cut(s) 235, 375, 703
HpyCH4IV ACGT 2 cut(s) 324, 543
HpyCH4V TGCA 6 cut(s) 286, 298, 362, 371, 482, 580
HpyF10VI GCNNNNNNNGC 5 cut(s) 124, 343, 359, 368, 586
HpySE526I ACGT 2 cut(s) 324, 543
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 9 cut(s) 217, 265, 273, 286, 302, 415, 531, 575, 664
KpnI GGTACC 1 cut(s) 654
Ksp22I TGATCA 1 cut(s) 210
KspI CCGCGG 1 cut(s) 455
Kzo9I GATC 3 cut(s) 94, 210, 669
LmnI GCTCC 2 cut(s) 358, 419
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 128
MaeII ACGT 2 cut(s) 324, 543
MaeIII GTNAC 2 cut(s) 223, 703
MalI GATC 3 cut(s) 96, 212, 671
MboI GATC 3 cut(s) 94, 210, 669
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 1 cut(s) 196
MlyI GAGTC 2 cut(s) 143, 196
MmeI TCCRAC 2 cut(s) 89, 369
MnlI CCTC 2 cut(s) 45, 385
Mph1103I ATGCAT 2 cut(s) 300, 364
MseI TTAA 2 cut(s) 206, 519
MslI CAYNNNNRTG 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 1 cut(s) 510
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 5 cut(s) 124, 343, 359, 368, 586
NcoI CCATGG 2 cut(s) 411, 527
NdeI CATATG 2 cut(s) 364, 695
NdeII GATC 3 cut(s) 94, 210, 669
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 9 cut(s) 217, 265, 273, 286, 302, 415, 531, 575, 664
NlaIV GGNNCC 4 cut(s) 354, 421, 533, 652
NmuCI GTSAC 2 cut(s) 223, 703
NsiI ATGCAT 2 cut(s) 300, 364
NspI RCATGY 1 cut(s) 664
NspV TTCGAA 1 cut(s) 552
PagI TCATGA 2 cut(s) 213, 571
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PflMI CCANNNNNTGG 1 cut(s) 388
PleI GAGTC 2 cut(s) 142, 195
PpsI GAGTC 2 cut(s) 142, 195
PshAI GACNNNNGTC 1 cut(s) 221
PspFI CCCAGC 2 cut(s) 333, 615
PspN4I GGNNCC 4 cut(s) 354, 421, 533, 652
PspPI GGNCC 2 cut(s) 235, 699
PstNI CAGNNNCTG 1 cut(s) 244
RsaI GTAC 3 cut(s) 278, 377, 652
RsaNI GTAC 3 cut(s) 277, 376, 651
RseI CAYNNNNRTG 1 cut(s) 665
SacII CCGCGG 1 cut(s) 455
SaqAI TTAA 2 cut(s) 206, 519
Sau3AI GATC 3 cut(s) 94, 210, 669
Sau96I GGNCC 2 cut(s) 235, 699
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 2 cut(s) 143, 196
SduI GDGCHC 1 cut(s) 373
SetI ASST 6 cut(s) 9, 253, 327, 431, 546, 656
SfaNI GCATC 1 cut(s) 346
Sfr303I CCGCGG 1 cut(s) 455
SfuI TTCGAA 1 cut(s) 552
SgrBI CCGCGG 1 cut(s) 455
SinI GGWCC 2 cut(s) 235, 699
SmiMI CAYNNNNRTG 1 cut(s) 665
Sse9I AATT 1 cut(s) 196
SsiI CCGC 4 cut(s) 62, 416, 452, 454
SspMI CTAG 1 cut(s) 128
StyI CCWWGG 2 cut(s) 411, 527
TaaI ACNGT 3 cut(s) 235, 375, 703
TaiI ACGT 2 cut(s) 327, 546
TaqI TCGA 3 cut(s) 157, 461, 552
TasI AATT 1 cut(s) 196
TatI WGTACW 2 cut(s) 276, 375
Tru1I TTAA 2 cut(s) 206, 519
Tru9I TTAA 2 cut(s) 206, 519
TscAI CASTG 2 cut(s) 378, 501
TseFI GTSAC 2 cut(s) 223, 703
Tsp45I GTSAC 2 cut(s) 223, 703
TspDTI ATGAA 3 cut(s) 17, 271, 699
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 378, 501
Van91I CCANNNNNTGG 1 cut(s) 388
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 2 cut(s) 235, 699
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 2 cut(s) 493, 590
XspI CTAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 278
Zsp2I ATGCAT 2 cut(s) 300, 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.