RchiOBHm_Chr7g0211541

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001109
Physical Location & Seq
Forward (+)
28655944 .. 28657713
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18925

Sequence Viewer

Length: 732 bp
ATGGGAATAGCTTCTGATGAAATTCTGGGGATGTTTATCCCCATAATACTGTACTGGCTTTACGCTGCCGTAATAATATCCATTGAAAAATTAGTCCCCAAGTATAAACTGCGAAGCTCAGAGGAGCAAGACAAGAGAAACCACCTCGTCTCCAAAGGGACAGTAATCAAAGGTGTTGTCCTCCAGCAAGCACTGCAGGCTTGCACTGCCACACTCTTGTATATTCTGACAAACCGTGACAATGATGCTGCTGCTTCTTTTCTGATAGCTATGGTGGTGATGAACACATGGCAGTACTTCATGCACAGATATATGCATGAGAACAAGTTTCTTTACAAGCACGTCCACTCCCAGCATCACAGGCTGGTGGCTCCGTATGCATATGGTGCACTGTACAACCACCCGTTGGAGGGTCTTCTCGTGGATACCATGGCGGGAGCCGTCAGCTTCTTGGTCTCTGGGATGTCTCCGCGGACTTCGATTTTCTTCTTCTCCTTTGCAACCATCAAAGCAGTGGACGACCACTCCGGCCTATTTAACTTTCCATGGCACCCGTTTCACGTCTTATTCGAAAACAATAGCCTGTATCATGATTTGCACCATCAGCCTTCTGGGATAAAGTATAACTACTCCCGGCCATTCTTCGTTTTTTGGGATAAGATACTCGGTACCTATCACATGCCAATGATCCCACCATCTTATGAAGACGCCATATGGACCGTCACGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

243

Amino Acids

28.07

Weight (kDa)

7.91

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 87 - 224 1.1e-24 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 2 cut(s) 549, 668
AccB7I CCANNNNNTGG 1 cut(s) 406
AccII CGCG 1 cut(s) 472
AciI CCGC 3 cut(s) 434, 470, 472
AclWI GGATC 1 cut(s) 682
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 4 cut(s) 53, 296, 395, 670
AfiI CCNNNNNNNGG 3 cut(s) 406, 409, 410
AgsI TTSAA 1 cut(s) 86
AjiI CACGTC 2 cut(s) 343, 562
AluBI AGCT 4 cut(s) 11, 117, 269, 447
AluI AGCT 4 cut(s) 11, 117, 269, 447
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 3 cut(s) 154, 460, 471
Alw44I GTGCAC 1 cut(s) 387
AlwI GGATC 1 cut(s) 682
AoxI GGCC 2 cut(s) 529, 635
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 65, 248, 251
ApoI RAATTY 1 cut(s) 21
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
Asp700I GAANNNNTTC 1 cut(s) 10
Asp718I GGTACC 1 cut(s) 668
AspS9I GGNCC 1 cut(s) 717
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 1 cut(s) 289
AsuII TTCGAA 1 cut(s) 570
AvaII GGWCC 1 cut(s) 717
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 2 cut(s) 549, 668
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 407, 711
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 52, 235, 238
BccI CCATC 3 cut(s) 512, 609, 703
BceAI ACGGC 2 cut(s) 53, 425
BciVI GTATCC 1 cut(s) 418
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 3 cut(s) 154, 460, 471
BfmI CTRYAG 1 cut(s) 194
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 3 cut(s) 66, 249, 252
BlsI GCNGC 3 cut(s) 67, 250, 253
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 634
Bme18I GGWCC 1 cut(s) 717
BmgBI CACGTC 2 cut(s) 343, 562
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 4 cut(s) 372, 439, 551, 670
BmrFI CCNGG 1 cut(s) 634
BmsI GCATC 2 cut(s) 235, 364
BpiI GAAGAC 2 cut(s) 407, 711
BpmI CTGGAG 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 634
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 3 cut(s) 429, 470, 545
BsaXI ACNNNNNCTCC 6 cut(s) 134, 164, 332, 362, 509, 539
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 409, 410
Bse1I ACTGG 1 cut(s) 59
Bse8I GATNNNNATC 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 429, 470, 545
BseGI GGATG 2 cut(s) 36, 468
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 406, 409, 410
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 3 cut(s) 52, 235, 238
BseYI CCCAGC 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 472
BshFI GGCC 2 cut(s) 531, 637
BshNI GGYRCC 2 cut(s) 549, 668
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 2 cut(s) 528, 634
BslFI GGGAC 2 cut(s) 80, 172
BslI CCNNNNNNNGG 3 cut(s) 406, 409, 410
BsmAI GTCTC 3 cut(s) 154, 460, 471
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 80, 172
BsnI GGCC 2 cut(s) 531, 637
Bso31I GGTCTC 1 cut(s) 460
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 391
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 1 cut(s) 687
Bsp19I CCATGG 2 cut(s) 429, 545
BspACI CCGC 3 cut(s) 434, 470, 472
BspANI GGCC 2 cut(s) 531, 637
BspCNI CTCAG 1 cut(s) 131
BspFNI CGCG 1 cut(s) 472
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 4 cut(s) 372, 439, 551, 670
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 1 cut(s) 682
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 2 cut(s) 549, 668
BspTNI GGTCTC 1 cut(s) 460
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 429, 470, 545
BssMI GATC 1 cut(s) 687
BssNI GRCGYC 1 cut(s) 708
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 2 cut(s) 429, 545
Bst2BI CACGAG 1 cut(s) 419
Bst4CI ACNGT 5 cut(s) 51, 163, 236, 393, 721
BstACI GRCGYC 1 cut(s) 708
BstAPI GCANNNNNTGC 2 cut(s) 193, 386
BstAUI TGTACA 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 3 cut(s) 189, 198, 202
BstDEI CTNAG 1 cut(s) 118
BstDSI CCRYGG 3 cut(s) 429, 470, 545
BstF5I GGATG 2 cut(s) 36, 468
BstFNI CGCG 1 cut(s) 472
BstKTI GATC 1 cut(s) 690
BstMAI GTCTC 3 cut(s) 154, 460, 471
BstMBI GATC 1 cut(s) 687
BstMWI GCNNNNNNNGC 7 cut(s) 193, 197, 206, 361, 377, 386, 604
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 1 cut(s) 632
BstSFI CTRYAG 1 cut(s) 194
BstSLI GKGCMC 1 cut(s) 391
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 3 cut(s) 52, 235, 238
BstV2I GAAGAC 2 cut(s) 407, 711
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 531, 637
BtgI CCRYGG 3 cut(s) 429, 470, 545
BtrI CACGTC 2 cut(s) 343, 562
BtsCI GGATG 2 cut(s) 36, 468
BtsI GCAGTG 3 cut(s) 191, 204, 519
BtsIMutI CAGTG 4 cut(s) 191, 204, 389, 519
Cac8I GCNNGC 3 cut(s) 189, 198, 202
CciI TCATGA 1 cut(s) 589
Cfr13I GGNCC 1 cut(s) 717
Cfr42I CCGCGG 1 cut(s) 473
CseI GACGC 1 cut(s) 716
Csp6I GTAC 4 cut(s) 52, 295, 394, 669
CviAII CATG 7 cut(s) 288, 301, 317, 430, 546, 590, 679
CviQI GTAC 4 cut(s) 52, 295, 394, 669
DdeI CTNAG 1 cut(s) 118
DpnI GATC 1 cut(s) 689
DpnII GATC 1 cut(s) 687
EaeI YGGCCR 1 cut(s) 635
Eco130I CCWWGG 2 cut(s) 429, 545
Eco31I GGTCTC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 429, 545
EcoT22I ATGCAT 2 cut(s) 318, 382
ErhI CCWWGG 2 cut(s) 429, 545
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 7 cut(s) 291, 304, 320, 433, 549, 593, 682
FaqI GGGAC 2 cut(s) 80, 172
FatI CATG 7 cut(s) 287, 300, 316, 429, 545, 589, 678
FauI CCCGC 1 cut(s) 427
FauNDI CATATG 2 cut(s) 382, 713
Fnu4HI GCNGC 3 cut(s) 66, 249, 252
FokI GGATG 2 cut(s) 43, 475
Fsp4HI GCNGC 3 cut(s) 66, 249, 252
GluI GCNGC 3 cut(s) 66, 249, 252
GsaI CCCAGC 1 cut(s) 355
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 2 cut(s) 531, 637
HapII CCGG 2 cut(s) 528, 634
HgaI GACGC 1 cut(s) 716
Hin1I GRCGYC 1 cut(s) 708
Hin1II CATG 7 cut(s) 291, 304, 320, 433, 549, 593, 682
HpaII CCGG 2 cut(s) 528, 634
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 3 cut(s) 346, 389, 517
Hpy188I TCNGA 4 cut(s) 16, 121, 228, 264
Hpy188III TCNNGA 2 cut(s) 590, 724
Hpy8I GTNNAC 3 cut(s) 346, 389, 517
HpyAV CCTTC 1 cut(s) 618
HpyCH4III ACNGT 5 cut(s) 51, 163, 236, 393, 721
HpyCH4IV ACGT 2 cut(s) 342, 561
HpyCH4V TGCA 8 cut(s) 196, 204, 304, 316, 380, 389, 500, 598
HpyF10VI GCNNNNNNNGC 7 cut(s) 193, 197, 206, 361, 377, 386, 604
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 2 cut(s) 342, 561
Hsp92I GRCGYC 1 cut(s) 708
Hsp92II CATG 7 cut(s) 291, 304, 320, 433, 549, 593, 682
KpnI GGTACC 1 cut(s) 672
KspI CCGCGG 1 cut(s) 473
Kzo9I GATC 1 cut(s) 687
LmnI GCTCC 3 cut(s) 124, 376, 437
Lsp1109I GCAGC 3 cut(s) 52, 235, 238
LweI GCATC 2 cut(s) 235, 364
MaeII ACGT 2 cut(s) 342, 561
MaeIII GTNAC 2 cut(s) 236, 721
MalI GATC 1 cut(s) 689
MboI GATC 1 cut(s) 687
MboII GAAGA 5 cut(s) 407, 478, 481, 634, 716
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 2 cut(s) 21, 89
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 4 cut(s) 115, 155, 191, 403
Mph1103I ATGCAT 2 cut(s) 318, 382
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 2 cut(s) 528, 634
MspR9I CCNGG 1 cut(s) 634
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 7 cut(s) 193, 197, 206, 361, 377, 386, 604
NciI CCSGG 1 cut(s) 634
NcoI CCATGG 2 cut(s) 429, 545
NdeI CATATG 2 cut(s) 382, 713
NdeII GATC 1 cut(s) 687
NlaIII CATG 7 cut(s) 291, 304, 320, 433, 549, 593, 682
NlaIV GGNNCC 4 cut(s) 372, 439, 551, 670
NmuCI GTSAC 2 cut(s) 236, 721
NsiI ATGCAT 2 cut(s) 318, 382
NspI RCATGY 1 cut(s) 682
NspV TTCGAA 1 cut(s) 570
PagI TCATGA 1 cut(s) 589
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 10
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 3 cut(s) 67, 250, 253
PspFI CCCAGC 1 cut(s) 351
PspN4I GGNNCC 4 cut(s) 372, 439, 551, 670
PspPI GGNCC 1 cut(s) 717
PstI CTGCAG 1 cut(s) 198
RsaI GTAC 4 cut(s) 53, 296, 395, 670
RsaNI GTAC 4 cut(s) 52, 295, 394, 669
RseI CAYNNNNRTG 1 cut(s) 683
SacII CCGCGG 1 cut(s) 473
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 3 cut(s) 66, 249, 252
Sau3AI GATC 1 cut(s) 687
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 634
SduI GDGCHC 1 cut(s) 391
SetI ASST 9 cut(s) 13, 119, 147, 175, 271, 345, 449, 564, 674
SfaNI GCATC 2 cut(s) 235, 364
SfcI CTRYAG 1 cut(s) 194
Sfr303I CCGCGG 1 cut(s) 473
SfuI TTCGAA 1 cut(s) 570
SgrBI CCGCGG 1 cut(s) 473
SinI GGWCC 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 683
Sse9I AATT 2 cut(s) 21, 89
SsiI CCGC 3 cut(s) 434, 470, 472
StyD4I CCNGG 1 cut(s) 632
StyI CCWWGG 2 cut(s) 429, 545
TaaI ACNGT 5 cut(s) 51, 163, 236, 393, 721
TaiI ACGT 2 cut(s) 345, 564
TaqI TCGA 2 cut(s) 479, 570
TasI AATT 2 cut(s) 21, 89
TatI WGTACW 3 cut(s) 51, 294, 393
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 4 cut(s) 198, 211, 396, 519
TseFI GTSAC 2 cut(s) 236, 721
TseI GCWGC 3 cut(s) 65, 248, 251
Tsp45I GTSAC 2 cut(s) 236, 721
TspDTI ATGAA 4 cut(s) 33, 289, 296, 717
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 4 cut(s) 198, 211, 396, 519
Van91I CCANNNNNTGG 1 cut(s) 406
VneI GTGCAC 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 1 cut(s) 21
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 511, 608
XmnI GAANNNNTTC 1 cut(s) 10
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 318, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.