FvH4_5g23420

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000888
Physical Location & Seq
Reverse (-)
14763406 .. 14766264
2859 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23420.t1

Sequence Viewer

Length: 2859 bp
ATGGCGGAGAGTGCAGTCAAGTTTCTGCTTGACAAGCTTGCACCCCTTTTTGAAAATGATTTGCAACTTTTGAGAGGAGGGGTTAGGGAAGAAATTGTTTATCTAAGAGGAGAGCTGGAGCGGATGACGGCTTTTCTGAGGGTTGCTGATGCATCTGAAGAGAGTGATGAGGAGCTCAAAGTTTGGGTCAAGCAACTAAGAGACATTGCTCACGACAGTGAAGATGTTCTTGATGAATTTACACTTCTGCAGGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAGATTTTCTTGCTGCATTAAGAACACAAAAGCTCGGTATCGTATTGCTTCTGAATTACAAGGCATCAACTTGAGAATCAGAAAAATCTCGGAGGTTCACAAGAGACTCCGTCACAAATTCAATATGGCTGAACAAGGCGCAGGCTCCACCAGTGCATGCAATACATGGGATGACCATCAAGGCGATGCTCTACTTCTAGAGAAGACTGATATTGTAGGCATTGACAAGCTCATACAGCAGCTGGTTGGGTGGCTAGTCAAAGGTGGTTCTGGACGTGAAGTAGTTTCTGTGGCTGGAATCGGAGGGATGGGGAAAACTACCCTGGCCAAGCAAGTCTATGATGCTGCAGAAGTGAAGAAACATTTCAAAGTTTGTGCTTGGATCACTGTTACTCGGTCTTTCAAACTTGGAGAACTCCTAAAAGACATGGTTCATCAACTCTACAACGCAATCCGCAGGCCTGTTCCAGCAGGAATCAATAATATGAATAACAATCACTTGAAAACAATAATCAAGGACTTCCTGCAGAAAAGGAGATATCTCATTGTTTTAGATGATGTATCGCACTTGTATGGATGGGATGCTGTGAAATATGCCTTGCCTAACAATACTTCTGGTAGCCGAGTCATTCTCACAACTCGAAATGCTGAAGTAGCCTCTACGACCAGTGTAGAATCTGGAGGGAAAGTCTACAATTTGGAGCCCTTACCCCAGTCGGAGTCTTGGGAACTTTTATGCAAGAAGACATTCCAGGGGAAATCTTGCCCTCCTCATTTAGAGGAAATCTGTACTTATGCTTTGAAGAAGTGTGAGGGGATGCCCCTTGCAATTGTGGCGGTCAGTGGTGTCTTGTCTACCAAGGACCAGCGTCGGATTGATGAGTGGGATATGGTTTGCCATAGTCTTGGAGCTGAAATTCAGGGCAATGACAAACTGAGGGACTTAAAAAAAGTACTTTCTCTCAGTTTCAATGATTTACCATACTATCTAAAATCTTGCTTCTTGTACCTGAGCATCTTTCCTGAGGATTATCTAATTGAGCATATGAGGCTTGTTCGATTATGGATGGCAGAGGGGTTTGTCGAACCAAAAGATGGCAAAACATTGGAAGATGTTGCGGAGGACTACCTCAGTGAATTACTGAATAGAGGCATGATTCAAGCTGCTGACACAACCTCTGATGGAAGGGTCAAGAACATTCGTGTTCATGACCTTTTCCGAGAGATGATCACCTCTAAGATAAGAGATCAGAACTTTGCCACAATTTCTAAAGAGCAAAATGTTCCTTGGCCCGATAAAGTGCGCCGCCTATCATTGCACAACTCAATGCAGTATGTACAGAAAAACAGGTGTGTTTCTCAACTGCGTTCTCTATTTATGTTTAGAGTTCCTGAGAAGCCATTACTACAAAAGTATTTCCCTGGAGGGTTCAGACTTCTTAATGTTTTAGACTTGCAAAATGCGCCTCTAAATGTTTTCCCGGTTGAAATTATAAACCTTTTCTTTTTGAAGTATCTGAGTTTAAAAGGTACCAAGGTGAAAACCATTCCAAGGTTCATTGGGAAGCTTCAGAACCTAGAGACCTTGGATCTTAAACATTCTCTGGTTACTGAACTCCCAGTTGAAATCTTGAAGCTTGAACATCTACGTCATCTCCTGGTATATCGTTATGAATTTGTACCTTATGGAGACTTTCACTCAAAATATGGTTTCAAGGTACTAGTGAAAATTGGGGCTTTGACATCCCTTCAAAAGCTCTGTTTCATCAAGGCAAACGAAGATGGTGGTGCCATATTAAAAGAGCTTGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTACAGATGAGGAAAGAAGATGGAAAAGTCCTTTGCTCATCGATTGAAAAGCTGAACAAACTTTGCGCGTTATCCATAACTTCAGTAGAAGAGGATGAGATCATTGATCTGCAGCATCTTTCTTCTCCTCCTCTACTGCTTCAGCGCCTCTACCTGCGAGGACGGTTAGAGACAATACCTCACTGGATTCCTTCTCTGCATAGCCTTGTCAGGTTATATCTGAAATGGAGCAGGTTGAAGGATGATCCACTTGTATTCCTTCAATATTTGCCCAATCTAGTACACCTCGAACTATCTCAGGTGTTTGAAGGAGAGACATTATGCTTTGAAGCTGGAGGGTTCAAGAAGCTCAAACATTTAGGTATCGACGAATTTGAGGGACTGAAGTACATACAGGTGCAGTTGGGAGCAATGCCTTCTGTGAAAAAGTTAAGCATCCAGCGCTGCAAGTTATTGGAGAAGGTGCCATCAGGCATTGAACACCTGAGCAAACTGAAGGTGCTCGAGTTTTTTGACATGCCAGAAACATTGATCAAGACGCTACGTCCACATGAGAAGGACAATGACTATTGGAAAGTCGCACACATTCCAGAAGTTTATTTCACATACTGGAAAGAGTGTGGATGGGAGGTCTATCCTCTAGAGGGTTTGAGTGAAGGAGAGAATTTTCCTCATGATCAGCCTAGTTCTGTCATGAAGAGCCATGAACTTGAAACTCGTTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

953

Amino Acids

109.2

Weight (kDa)

8.48

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 88 3.3e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 351 2e-40 NB-ARC domain
WHD_DRP PF23559 436 - 507 1.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 879 2.3e-47 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1784
Acc36I ACCTGC 2 cut(s) 2292, 2352
Acc65I GGTACC 1 cut(s) 1820
AccB1I GGYRCC 3 cut(s) 1820, 2078, 2593
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccBSI CCGCTC 1 cut(s) 121
AccI GTMKAC 2 cut(s) 981, 1145
AccII CGCG 1 cut(s) 2198
AciI CCGC 6 cut(s) 5, 121, 745, 1127, 1409, 1597
AclWI GGATC 3 cut(s) 680, 1887, 2369
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 6 cut(s) 236, 407, 1206, 1964, 2501, 2794
AcuI CTGAAG 7 cut(s) 177, 960, 1844, 2196, 2255, 2534, 2645
AfeI AGCGCT 1 cut(s) 2573
AfiI CCNNNNNNNGG 3 cut(s) 1385, 1842, 2774
AhdI GACNNNNNGTC 1 cut(s) 2673
AhlI ACTAGT 2 cut(s) 2011, 2104
AjiI CACGTC 1 cut(s) 566
AjnI CCWGG 4 cut(s) 612, 1041, 1711, 1947
AleI CACNNNNGTG 1 cut(s) 216
Alw21I GWGCWC 2 cut(s) 177, 2634
Alw26I GTCTC 6 cut(s) 195, 388, 1865, 1974, 2294, 2438
AlwI GGATC 3 cut(s) 680, 1887, 2369
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 2633
Aor51HI AGCGCT 1 cut(s) 2573
AoxI GGCC 3 cut(s) 615, 749, 1580
ApeKI GCWGC 6 cut(s) 303, 529, 635, 1454, 2242, 2574
ApoI RAATTY 6 cut(s) 236, 407, 1206, 1964, 2501, 2794
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
Asp700I GAANNNNTTC 3 cut(s) 225, 653, 1037
Asp718I GGTACC 1 cut(s) 1820
AspLEI GCGC 6 cut(s) 431, 1596, 1756, 2198, 2277, 2574
AspS9I GGNCC 2 cut(s) 1153, 1581
AsuC2I CCSGG 1 cut(s) 1772
AsuHPI GGTGA 2 cut(s) 1513, 1840
AvaI CYCGRG 1 cut(s) 2633
AvaII GGWCC 1 cut(s) 1153
AxyI CCTNAGG 1 cut(s) 1314
BaeI ACNNNNGTAYC 2 cut(s) 1812, 1845
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 3 cut(s) 1820, 2078, 2593
BanII GRGCYC 2 cut(s) 177, 996
BbsI GAAGAC 2 cut(s) 500, 1040
Bbv12I GWGCWC 2 cut(s) 177, 2634
BbvI GCAGC 6 cut(s) 290, 541, 622, 1441, 2254, 2561
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 4 cut(s) 614, 1043, 1713, 1949
BclI TGATCA 4 cut(s) 259, 1518, 2661, 2806
BcnI CCSGG 1 cut(s) 1772
BcoDI GTCTC 6 cut(s) 195, 388, 1865, 1974, 2294, 2438
BcuI ACTAGT 2 cut(s) 2011, 2104
BfaI CTAG 8 cut(s) 488, 545, 1868, 2012, 2105, 2408, 2771, 2814
BfmI CTRYAG 4 cut(s) 248, 636, 815, 2240
BfoI RGCGCY 2 cut(s) 2278, 2575
BfuAI ACCTGC 2 cut(s) 2292, 2352
BisI GCNGC 7 cut(s) 304, 530, 636, 1455, 1597, 2243, 2575
BlsI GCNGC 7 cut(s) 305, 531, 637, 1456, 1598, 2244, 2576
BmcAI AGTACT 1 cut(s) 1245
Bme1390I CCNGG 5 cut(s) 614, 1043, 1713, 1772, 1949
Bme18I GGWCC 1 cut(s) 1153
BmeRI GACNNNNNGTC 1 cut(s) 2673
BmeT110I CYCGRG 1 cut(s) 2633
BmgBI CACGTC 1 cut(s) 566
BmgT120I GGNCC 2 cut(s) 1153, 1581
BmiI GGNNCC 5 cut(s) 436, 993, 1822, 2080, 2595
BmrFI CCNGG 5 cut(s) 614, 1043, 1713, 1772, 1949
BmrI ACTGGG 2 cut(s) 997, 1904
BmuI ACTGGG 2 cut(s) 997, 1904
BoxI GACNNNNGTC 1 cut(s) 1158
BpiI GAAGAC 2 cut(s) 500, 1040
BplI GAGNNNNNCTC 2 cut(s) 906, 938
BpmI CTGGAG 4 cut(s) 137, 990, 1734, 2484
Bpu10I CCTNAGC 2 cut(s) 1301, 2615
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 1772
Bsa29I ATCGAT 1 cut(s) 2171
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 1865
BsaJI CCNNGG 8 cut(s) 612, 1042, 1149, 1577, 1711, 1824, 1841, 1875
BsaXI ACNNNNNCTCC 4 cut(s) 963, 993, 1929, 1959
Bsc4I CCNNNNNNNGG 3 cut(s) 1385, 1842, 2774
Bse1I ACTGG 6 cut(s) 441, 957, 1003, 1910, 2318, 2744
Bse21I CCTNAGG 1 cut(s) 1314
Bse3DI GCAATG 4 cut(s) 204, 1222, 1604, 2547
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 4 cut(s) 614, 1043, 1713, 1949
BseCI ATCGAT 1 cut(s) 2171
BseDI CCNNGG 8 cut(s) 612, 1042, 1149, 1577, 1711, 1824, 1841, 1875
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 1385, 1842, 2774
BseMI GCAATG 4 cut(s) 204, 1222, 1604, 2547
BseNI ACTGG 6 cut(s) 441, 957, 1003, 1910, 2318, 2744
BseRI GAGGAG 6 cut(s) 90, 123, 185, 1050, 2247, 2250
BseXI GCAGC 6 cut(s) 290, 541, 622, 1441, 2254, 2561
BsgI GTGCAG 2 cut(s) 33, 2549
Bsh1236I CGCG 1 cut(s) 2198
BshFI GGCC 3 cut(s) 617, 751, 1582
BshNI GGYRCC 3 cut(s) 1820, 2078, 2593
BshVI ATCGAT 1 cut(s) 2171
BsiHKAI GWGCWC 2 cut(s) 177, 2634
BsiHKCI CYCGRG 1 cut(s) 2633
BsiSI CCGG 1 cut(s) 1772
BslFI GGGAC 2 cut(s) 1244, 2523
BslI CCNNNNNNNGG 3 cut(s) 1385, 1842, 2774
BsmAI GTCTC 6 cut(s) 195, 388, 1865, 1974, 2294, 2438
BsmFI GGGAC 2 cut(s) 1244, 2523
BsnI GGCC 3 cut(s) 617, 751, 1582
Bso31I GGTCTC 1 cut(s) 1865
BsoBI CYCGRG 1 cut(s) 2633
Bsp1286I GDGCHC 3 cut(s) 177, 996, 2634
Bsp1407I TGTACA 2 cut(s) 1627, 2131
BspACI CCGC 6 cut(s) 5, 121, 745, 1127, 1409, 1597
BspANI GGCC 3 cut(s) 617, 751, 1582
BspDI ATCGAT 1 cut(s) 2171
BspFNI CGCG 1 cut(s) 2198
BspHI TCATGA 3 cut(s) 1498, 2803, 2823
BspLI GGNNCC 5 cut(s) 436, 993, 1822, 2080, 2595
BspMAI CTGCAG 4 cut(s) 252, 640, 819, 2244
BspMI ACCTGC 2 cut(s) 2292, 2352
BspPI GGATC 3 cut(s) 680, 1887, 2369
BspQI GCTCTTC 1 cut(s) 2822
BspT107I GGYRCC 3 cut(s) 1820, 2078, 2593
BspTNI GGTCTC 1 cut(s) 1865
BsrBI CCGCTC 1 cut(s) 121
BsrDI GCAATG 4 cut(s) 204, 1222, 1604, 2547
BsrGI TGTACA 2 cut(s) 1627, 2131
BsrI ACTGG 6 cut(s) 441, 957, 1003, 1910, 2318, 2744
BssECI CCNNGG 8 cut(s) 612, 1042, 1149, 1577, 1711, 1824, 1841, 1875
BssT1I CCWWGG 5 cut(s) 1149, 1577, 1824, 1841, 1875
Bst2UI CCWGG 4 cut(s) 614, 1043, 1713, 1949
Bst4CI ACNGT 3 cut(s) 218, 679, 2295
Bst6I CTCTTC 3 cut(s) 153, 2214, 2822
BstAUI TGTACA 2 cut(s) 1627, 2131
BstC8I GCNNGC 5 cut(s) 39, 252, 433, 448, 749
BstFNI CGCG 1 cut(s) 2198
BstH2I RGCGCY 2 cut(s) 2278, 2575
BstHHI GCGC 6 cut(s) 431, 1596, 1756, 2198, 2277, 2574
BstMAI GTCTC 6 cut(s) 195, 388, 1865, 1974, 2294, 2438
BstMWI GCNNNNNNNGC 8 cut(s) 11, 34, 526, 944, 1124, 1339, 1753, 2571
BstNI CCWGG 4 cut(s) 614, 1043, 1713, 1949
BstNSI RCATGY 2 cut(s) 450, 2650
BstPAI GACNNNNGTC 1 cut(s) 1158
BstSCI CCNGG 5 cut(s) 612, 1041, 1711, 1770, 1947
BstSFI CTRYAG 4 cut(s) 248, 636, 815, 2240
BstUI CGCG 1 cut(s) 2198
BstV1I GCAGC 6 cut(s) 290, 541, 622, 1441, 2254, 2561
BstV2I GAAGAC 2 cut(s) 500, 1040
BstX2I RGATCY 1 cut(s) 1879
BstXI CCANNNNNNTGG 1 cut(s) 1196
BstYI RGATCY 1 cut(s) 1879
Bsu15I ATCGAT 1 cut(s) 2171
Bsu36I CCTNAGG 1 cut(s) 1314
BsuRI GGCC 3 cut(s) 617, 751, 1582
BsuTUI ATCGAT 1 cut(s) 2171
BtgZI GCGATG 1 cut(s) 489
BtrI CACGTC 1 cut(s) 566
BtsIMutI CAGTG 7 cut(s) 223, 448, 675, 964, 1138, 1429, 2311
BveI ACCTGC 2 cut(s) 2292, 2352
Cac8I GCNNGC 5 cut(s) 39, 252, 433, 448, 749
CaiI CAGNNNCTG 1 cut(s) 532
CciI TCATGA 3 cut(s) 1498, 2803, 2823
CfoI GCGC 6 cut(s) 431, 1596, 1756, 2198, 2277, 2574
Cfr13I GGNCC 2 cut(s) 1153, 1581
ClaI ATCGAT 1 cut(s) 2171
CseI GACGC 2 cut(s) 1148, 2677
DraI TTTAAA 1 cut(s) 1815
DriI GACNNNNNGTC 1 cut(s) 2673
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 3 cut(s) 153, 2214, 2822
Eam1105I GACNNNNNGTC 1 cut(s) 2673
EarI CTCTTC 3 cut(s) 153, 2214, 2822
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 5 cut(s) 1149, 1577, 1824, 1841, 1875
Eco147I AGGCCT 1 cut(s) 751
Eco24I GRGCYC 2 cut(s) 177, 996
Eco31I GGTCTC 1 cut(s) 1865
Eco32I GATATC 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 1153
Eco47III AGCGCT 1 cut(s) 2573
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 7 cut(s) 177, 960, 1844, 2196, 2255, 2534, 2645
Eco81I CCTNAGG 1 cut(s) 1314
Eco88I CYCGRG 1 cut(s) 2633
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 4 cut(s) 612, 1041, 1711, 1947
EcoRV GATATC 1 cut(s) 830
EcoT14I CCWWGG 5 cut(s) 1149, 1577, 1824, 1841, 1875
EcoT22I ATGCAT 1 cut(s) 154
EcoT38I GRGCYC 2 cut(s) 177, 996
ErhI CCWWGG 5 cut(s) 1149, 1577, 1824, 1841, 1875
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 2 cut(s) 1244, 2523
FauNDI CATATG 1 cut(s) 1335
FbaI TGATCA 4 cut(s) 259, 1518, 2661, 2806
FblI GTMKAC 2 cut(s) 981, 1145
Fnu4HI GCNGC 7 cut(s) 304, 530, 636, 1455, 1597, 2243, 2575
FriOI GRGCYC 2 cut(s) 177, 996
Fsp4HI GCNGC 7 cut(s) 304, 530, 636, 1455, 1597, 2243, 2575
FspBI CTAG 8 cut(s) 488, 545, 1868, 2012, 2105, 2408, 2771, 2814
GlaI GCGC 6 cut(s) 430, 1595, 1755, 2197, 2276, 2573
GluI GCNGC 7 cut(s) 304, 530, 636, 1455, 1597, 2243, 2575
GsuI CTGGAG 4 cut(s) 137, 990, 1734, 2484
HaeII RGCGCY 2 cut(s) 2278, 2575
HaeIII GGCC 3 cut(s) 617, 751, 1582
HapII CCGG 1 cut(s) 1772
HgaI GACGC 2 cut(s) 1148, 2677
HhaI GCGC 6 cut(s) 431, 1596, 1756, 2198, 2277, 2574
Hin6I GCGC 6 cut(s) 429, 1594, 1754, 2196, 2275, 2572
HinP1I GCGC 6 cut(s) 429, 1594, 1754, 2196, 2275, 2572
HindIII AAGCTT 3 cut(s) 35, 1856, 1925
HinfI GANTC 9 cut(s) 366, 396, 588, 765, 915, 965, 1010, 1447, 2317
HpaII CCGG 1 cut(s) 1772
HphI GGTGA 2 cut(s) 1513, 1840
Hpy166II GTNNAC 5 cut(s) 388, 982, 1146, 2413, 2678
Hpy8I GTNNAC 5 cut(s) 388, 982, 1146, 2413, 2678
Hpy99I CGWCG 2 cut(s) 1164, 2501
HpyCH4III ACNGT 3 cut(s) 218, 679, 2295
HpyCH4IV ACGT 3 cut(s) 565, 1939, 2674
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 34, 526, 944, 1124, 1339, 1753, 2571
HpySE526I ACGT 3 cut(s) 565, 1939, 2674
HspAI GCGC 6 cut(s) 429, 1594, 1754, 2196, 2275, 2572
KpnI GGTACC 1 cut(s) 1824
Ksp22I TGATCA 4 cut(s) 259, 1518, 2661, 2806
LguI GCTCTTC 1 cut(s) 2822
LmnI GCTCC 7 cut(s) 118, 172, 440, 991, 1199, 2358, 2537
Lsp1109I GCAGC 6 cut(s) 290, 541, 622, 1441, 2254, 2561
MaeI CTAG 8 cut(s) 488, 545, 1868, 2012, 2105, 2408, 2771, 2814
MaeII ACGT 3 cut(s) 565, 1939, 2674
MaeIII GTNAC 3 cut(s) 401, 679, 1897
MbiI CCGCTC 1 cut(s) 121
MfeI CAATTG 1 cut(s) 1119
MflI RGATCY 1 cut(s) 1879
MhlI GDGCHC 3 cut(s) 177, 996, 2634
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 3 cut(s) 390, 924, 1019
MmeI TCCRAC 3 cut(s) 987, 1142, 2831
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 154
MroXI GAANNNNTTC 3 cut(s) 225, 653, 1037
MscI TGGCCA 1 cut(s) 617
MseI TTAA 7 cut(s) 309, 1235, 1731, 1814, 1884, 2087, 2561
MslI CAYNNNNRTG 4 cut(s) 216, 261, 861, 2525
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 1 cut(s) 1772
MspR9I CCNGG 5 cut(s) 614, 1043, 1713, 1772, 1949
MunI CAATTG 1 cut(s) 1119
MvaI CCWGG 4 cut(s) 614, 1043, 1713, 1949
MvnI CGCG 1 cut(s) 2198
MwoI GCNNNNNNNGC 8 cut(s) 11, 34, 526, 944, 1124, 1339, 1753, 2571
NciI CCSGG 1 cut(s) 1772
NdeI CATATG 1 cut(s) 1335
NlaIV GGNNCC 5 cut(s) 436, 993, 1822, 2080, 2595
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 1 cut(s) 401
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 2 cut(s) 450, 2650
OliI CACNNNNGTG 1 cut(s) 216
PaeI GCATGC 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 2633
PagI TCATGA 3 cut(s) 1498, 2803, 2823
PceI AGGCCT 1 cut(s) 751
PciSI GCTCTTC 1 cut(s) 2822
PdmI GAANNNNTTC 3 cut(s) 225, 653, 1037
PfeI GAWTC 6 cut(s) 366, 588, 765, 965, 1447, 2317
PflFI GACNNNGTC 1 cut(s) 399
PflMI CCANNNNNTGG 1 cut(s) 1385
PkrI GCNGC 7 cut(s) 305, 531, 637, 1456, 1598, 2244, 2576
PleI GAGTC 3 cut(s) 390, 923, 1018
PpsI GAGTC 3 cut(s) 390, 923, 1018
PshAI GACNNNNGTC 1 cut(s) 1158
PsiI TTATAA 1 cut(s) 1784
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 4 cut(s) 612, 1041, 1711, 1947
PspGI CCWGG 4 cut(s) 612, 1041, 1711, 1947
PspN4I GGNNCC 5 cut(s) 436, 993, 1822, 2080, 2595
PspPI GGNCC 2 cut(s) 1153, 1581
PspXI VCTCGAGB 1 cut(s) 2633
PstI CTGCAG 4 cut(s) 252, 640, 819, 2244
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 1879
PsyI GACNNNGTC 1 cut(s) 399
PvuII CAGCTG 1 cut(s) 532
RseI CAYNNNNRTG 4 cut(s) 216, 261, 861, 2525
SacI GAGCTC 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 2822
SaqAI TTAA 7 cut(s) 309, 1235, 1731, 1814, 1884, 2087, 2561
SatI GCNGC 7 cut(s) 304, 530, 636, 1455, 1597, 2243, 2575
Sau96I GGNCC 2 cut(s) 1153, 1581
ScaI AGTACT 1 cut(s) 1245
SchI GAGTC 3 cut(s) 390, 924, 1019
ScrFI CCNGG 5 cut(s) 614, 1043, 1713, 1772, 1949
SduI GDGCHC 3 cut(s) 177, 996, 2634
SfcI CTRYAG 4 cut(s) 248, 636, 815, 2240
Sfr274I CTCGAG 1 cut(s) 2633
SinI GGWCC 1 cut(s) 1153
SlaI CTCGAG 1 cut(s) 2633
SmiMI CAYNNNNRTG 4 cut(s) 216, 261, 861, 2525
SmlI CTYRAG 2 cut(s) 361, 2633
SmoI CTYRAG 2 cut(s) 361, 2633
SpeI ACTAGT 2 cut(s) 2011, 2104
SphI GCATGC 1 cut(s) 450
SseBI AGGCCT 1 cut(s) 751
SsiI CCGC 6 cut(s) 5, 121, 745, 1127, 1409, 1597
SspI AATATT 1 cut(s) 2396
SspMI CTAG 8 cut(s) 488, 545, 1868, 2012, 2105, 2408, 2771, 2814
SstI GAGCTC 1 cut(s) 177
StuI AGGCCT 1 cut(s) 751
StyD4I CCNGG 5 cut(s) 612, 1041, 1711, 1770, 1947
StyI CCWWGG 5 cut(s) 1149, 1577, 1824, 1841, 1875
TaaI ACNGT 3 cut(s) 218, 679, 2295
TaiI ACGT 3 cut(s) 568, 1942, 2677
TaqI TCGA 7 cut(s) 931, 1348, 1374, 2171, 2418, 2496, 2634
TaqII GACCGA 1 cut(s) 675
TatI WGTACW 6 cut(s) 1079, 1243, 1627, 2131, 2410, 2517
TauI GCSGC 1 cut(s) 1599
TfiI GAWTC 6 cut(s) 366, 588, 765, 965, 1447, 2317
Tru1I TTAA 7 cut(s) 309, 1235, 1731, 1814, 1884, 2087, 2561
Tru9I TTAA 7 cut(s) 309, 1235, 1731, 1814, 1884, 2087, 2561
TscAI CASTG 7 cut(s) 223, 448, 682, 964, 1138, 1429, 2318
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 6 cut(s) 303, 529, 635, 1454, 2242, 2574
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 9 cut(s) 249, 713, 791, 1487, 1837, 1977, 2044, 2840, 2850
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 7 cut(s) 223, 448, 682, 964, 1138, 1429, 2318
Tth111I GACNNNGTC 1 cut(s) 399
Van91I CCANNNNNTGG 1 cut(s) 1385
VpaK11BI GGWCC 1 cut(s) 1153
XapI RAATTY 6 cut(s) 236, 407, 1206, 1964, 2501, 2794
XbaI TCTAGA 2 cut(s) 487, 2770
XceI RCATGY 2 cut(s) 450, 2650
XhoI CTCGAG 1 cut(s) 2633
XmiI GTMKAC 2 cut(s) 981, 1145
XmnI GAANNNNTTC 3 cut(s) 225, 653, 1037
XspI CTAG 8 cut(s) 488, 545, 1868, 2012, 2105, 2408, 2771, 2814
ZrmI AGTACT 1 cut(s) 1245
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.