Rw2G025080

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000888
Physical Location & Seq
Forward (+)
37204363 .. 37207207
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025080.1

Sequence Viewer

Length: 2718 bp
ATGGCGGAGAGTGCAGTAAAGTTTCTACTTGACAAGCTTGCACCTCTTTTCGAAAATGAGTTGCAACTTTTGAGGGGGGTTAGGGAAGAAATTTTGCATCTAAGAGGAGAACTGGAGCGCATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAGCTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACAAAAGTGAAGATGTTTTTGATGAATTTACCCTTCTGCAAGCACATGATCATGAGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGACTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAGGTGATGCTTTACTTCTAGAGAAAACTGATATAGTGGGCATAGACGAGCCTATTAAGCAGATGGTTGGGAGGCTGGTCAATAGTGGTTGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGAGAAAACTATCCTGGCGAAGCAAGTCTATGATGCAGCAGAAGTGAAGAAACATTTCAAATTACGTGCTTGGATCACTTTTACTCAGTCTTTCAGGCTAGCGGAACTTCTCAAAGACATCGTTCAACAGCTCCACCAAGCAATCCGAAGTCCAGTTCCACAAGAAATCAATAGCATGAATACCAACCAGCTGAAAACAATAATTAAAGACTTCCTACAGAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGTATGCACTGCCTAATTATATTTACGGCAGCAGAGTGATACTAACTACTCGAAATGCAGATATAGCCTCCACAACAAGCACGGAATCTGGAGGTGAAGTCTATACTTTGAAGCCCTTGCCCCTAGTGGAGTCATGGGAGCTCCTGTGCAAGAAGACATTCCAAGGGAATTCCTGCCCTCCTCATTTAGAGAAAATCTGTAACTGTGTTCTGAGAAAGTGTGAGGGGCTACCCCTTGCTATTGTGGCAATTAGTGGATCTCTGGCTGCTAAAGATAAGCGCAGAATTGATGAGTGGGAAATGGTTGGCCACAGTCTCGGGGCTGAAATTAAGGGTAATGACAAACTGCAGGACTTGAAGAAAGTACTTTCACTCAGTTTCAATGATTTACCATACTATCTAAAATCGTGCTTCTTGTACCTGAGCATCTTCCCAGAGGACCATCTAATTAAGCATATGAGGCTTATTCGATTATGGATGGCAGAGGGCTTCATTCAAGAAAAACAAGGAAAGACATTGGAAGAGGTTGCAGAGGATTACCTCAATGAACTATTGAATAGGAGCATGATTCAAGCAGCAGAGACAACAGCTGATGGAAGGGTCACAAAATTTCGTATCCATGATCTCTTCCGCGAAATTATCACCTCCAAGACAAGAAGTCAGAACTTTGCCACAATAGCCAAAGAGCAGAATGTAACATTGCCAGAAAAAATCCATCGCCTGTCAATACATAACACACTGCAATATGTTCAGACAAACAGGTGTGCTTCTCAACTACGTTCTCTTTTTATGTTCACAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGCGCTGCTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCAGTTGAAGTTGTGAATCTTTACTTTTTAAAGTATCTGAGCTTGAGGGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCAAGTTATTGAACTCCCAGTTGAAATTCTGAAACTCCAACATTTGCCCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTCTCACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGTATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGCAGCCCCTGCGTTCTCCTCCTCTATTGCTTCAGCATTTGTACTTGCGAGGCAGACTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTAGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTGCCCAATCTAGTACATCTTGAGTTGTCCGACGGATTTAAGAAGCTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCTTGTATTGAAAATCTAATCATCTGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGTTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCATTAGAGGGCTTGAGTGAGACACAAATCTCTTCTCAACCCAGTTCTGTCATGAAGAGCATTGGAACCGGTTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

905

Amino Acids

103.86

Weight (kDa)

8.59

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 1.5e-22 Rx N-terminal domain
NB-ARC PF00931 142 - 320 4.6e-38 NB-ARC domain
WHD_DRP PF23559 405 - 476 8.5e-27 Disease resistance protein Winged helix domain
LRR_14 PF23598 520 - 833 2.4e-38 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2583
Acc36I ACCTGC 2 cut(s) 344, 2259
AccII CGCG 1 cut(s) 1419
AciI CCGC 4 cut(s) 5, 167, 608, 1417
AclWI GGATC 4 cut(s) 587, 1051, 1794, 2276
AcoI YGGCCR 1 cut(s) 1093
AcsI RAATTY 5 cut(s) 90, 233, 955, 1394, 1824
AcuI CTGAAG 4 cut(s) 1751, 2103, 2162, 2282
AfaI GTAC 5 cut(s) 1152, 1205, 1660, 2189, 2319
AfiI CCNNNNNNNGG 1 cut(s) 367
AflII CTTAAG 2 cut(s) 1790, 2504
AgeI ACCGGT 1 cut(s) 2705
AhdI GACNNNNNGTC 1 cut(s) 1443
AhlI ACTAGT 1 cut(s) 2011
AjiI CACGTC 1 cut(s) 473
AjnI CCWGG 3 cut(s) 519, 1730, 1773
AleI CACNNNNGTG 1 cut(s) 213
Alw21I GWGCWC 2 cut(s) 174, 930
Alw26I GTCTC 4 cut(s) 192, 1106, 1361, 2651
AlwI GGATC 4 cut(s) 587, 1051, 1794, 2276
AlwNI CAGNNNCTG 3 cut(s) 1589, 1774, 2155
Ama87I CYCGRG 1 cut(s) 1103
AoxI GGCC 1 cut(s) 1093
ApeKI GCWGC 8 cut(s) 300, 542, 816, 1052, 1361, 1633, 1983, 2149
ApoI RAATTY 5 cut(s) 90, 233, 955, 1394, 1824
AsiGI ACCGGT 1 cut(s) 2705
Asp700I GAANNNNTTC 3 cut(s) 334, 560, 944
AspLEI GCGC 6 cut(s) 120, 1068, 1633, 1988, 2445, 2584
AspS9I GGNCC 3 cut(s) 370, 1225, 1915
AsuHPI GGTGA 5 cut(s) 392, 893, 1420, 1981, 2596
AsuII TTCGAA 1 cut(s) 51
AsuNHI GCTAGC 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 3 cut(s) 370, 1225, 1915
AxyI CCTNAGG 1 cut(s) 134
BaeI ACNNNNGTAYC 2 cut(s) 1719, 1752
BalI TGGCCA 1 cut(s) 1095
BanII GRGCYC 2 cut(s) 174, 930
BbsI GAAGAC 2 cut(s) 947, 2136
Bbv12I GWGCWC 2 cut(s) 174, 930
BbvI GCAGC 8 cut(s) 287, 554, 828, 1039, 1373, 1620, 1995, 2161
BceAI ACGGC 2 cut(s) 141, 829
BciT130I CCWGG 3 cut(s) 521, 1732, 1775
BciVI GTATCC 3 cut(s) 1412, 1720, 2217
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 4 cut(s) 192, 1106, 1361, 2651
BcuI ACTAGT 1 cut(s) 2011
BfaI CTAG 6 cut(s) 395, 605, 911, 2012, 2243, 2315
BfmI CTRYAG 2 cut(s) 722, 1133
BfoI RGCGCY 1 cut(s) 2446
BfrI CTTAAG 2 cut(s) 1790, 2504
BfuAI ACCTGC 2 cut(s) 344, 2259
BfuI GTATCC 3 cut(s) 1412, 1720, 2217
BisI GCNGC 8 cut(s) 301, 543, 817, 1053, 1362, 1634, 1984, 2150
BlsI GCNGC 8 cut(s) 302, 544, 818, 1054, 1363, 1635, 1985, 2151
BmcAI AGTACT 1 cut(s) 1152
Bme1390I CCNGG 3 cut(s) 521, 1732, 1775
Bme18I GGWCC 3 cut(s) 370, 1225, 1915
BmeRI GACNNNNNGTC 1 cut(s) 1443
BmeT110I CYCGRG 1 cut(s) 1103
BmgBI CACGTC 1 cut(s) 473
BmgT120I GGNCC 3 cut(s) 370, 1225, 1915
BmiI GGNNCC 1 cut(s) 2704
BmrFI CCNGG 3 cut(s) 521, 1732, 1775
BmrI ACTGGG 3 cut(s) 1673, 1811, 2673
BmsI GCATC 9 cut(s) 106, 136, 154, 312, 373, 529, 769, 1221, 2378
BmtI GCTAGC 1 cut(s) 608
BmuI ACTGGG 3 cut(s) 1673, 1811, 2673
BpiI GAAGAC 2 cut(s) 947, 2136
BpmI CTGGAG 3 cut(s) 134, 897, 2477
Bpu10I CCTNAGC 1 cut(s) 1208
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 6 cut(s) 1741, 1785, 2336, 2345, 2632, 2671
BsaAI YACGTR 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 949, 1731, 1975
BsaWI WCCGGW 1 cut(s) 2705
BsaXI ACNNNNNCTCC 6 cut(s) 870, 900, 917, 947, 2217, 2247
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 2 cut(s) 1622, 2705
Bse1I ACTGG 8 cut(s) 117, 659, 1679, 1817, 2208, 2225, 2460, 2679
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 3 cut(s) 201, 1484, 2569
BseBI CCWGG 3 cut(s) 521, 1732, 1775
BseDI CCNNGG 3 cut(s) 949, 1731, 1975
BseGI GGATG 6 cut(s) 757, 779, 784, 1269, 2075, 2284
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 3 cut(s) 201, 1484, 2569
BseMII CTCAG 7 cut(s) 125, 605, 989, 1174, 1199, 1581, 1706
BseNI ACTGG 8 cut(s) 117, 659, 1679, 1817, 2208, 2225, 2460, 2679
BseRI GAGGAG 6 cut(s) 120, 278, 745, 957, 2154, 2157
BseXI GCAGC 8 cut(s) 287, 554, 828, 1039, 1373, 1620, 1995, 2161
BseYI CCCAGC 2 cut(s) 1618, 2002
BsgI GTGCAG 2 cut(s) 33, 372
Bsh1236I CGCG 1 cut(s) 1419
BshFI GGCC 1 cut(s) 1095
BshTI ACCGGT 1 cut(s) 2705
BsiHKAI GWGCWC 2 cut(s) 174, 930
BsiHKCI CYCGRG 1 cut(s) 1103
BsiSI CCGG 2 cut(s) 1623, 2706
BslFI GGGAC 2 cut(s) 2450, 2573
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 4 cut(s) 192, 1106, 1361, 2651
BsmFI GGGAC 2 cut(s) 2450, 2573
BsmI GAATGC 2 cut(s) 1795, 2291
BsnI GGCC 1 cut(s) 1095
BsoBI CYCGRG 1 cut(s) 1103
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 174, 930
Bsp143I GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
BspACI CCGC 4 cut(s) 5, 167, 608, 1417
BspANI GGCC 1 cut(s) 1095
BspCNI CTCAG 7 cut(s) 126, 604, 990, 1173, 1200, 1582, 1707
BspFNI CGCG 1 cut(s) 1419
BspHI TCATGA 2 cut(s) 259, 2688
BspLI GGNNCC 1 cut(s) 2704
BspMAI CTGCAG 1 cut(s) 1137
BspMI ACCTGC 2 cut(s) 344, 2259
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 4 cut(s) 587, 1051, 1794, 2276
BspQI GCTCTTC 1 cut(s) 2687
BspT104I TTCGAA 1 cut(s) 51
BspTI CTTAAG 2 cut(s) 1790, 2504
BsrDI GCAATG 3 cut(s) 201, 1484, 2569
BsrFI RCCGGY 2 cut(s) 1622, 2705
BsrI ACTGG 8 cut(s) 117, 659, 1679, 1817, 2208, 2225, 2460, 2679
BssAI RCCGGY 2 cut(s) 1622, 2705
BssECI CCNNGG 3 cut(s) 949, 1731, 1975
BssMI GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
BssT1I CCWWGG 2 cut(s) 949, 1975
Bst2UI CCWGG 3 cut(s) 521, 1732, 1775
Bst4CI ACNGT 3 cut(s) 992, 1100, 1585
Bst6I CTCTTC 6 cut(s) 150, 722, 1302, 1418, 2674, 2687
BstAFI CTTAAG 2 cut(s) 1790, 2504
BstAPI GCANNNNNTGC 1 cut(s) 2155
BstBAI YACGTR 1 cut(s) 572
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 4 cut(s) 39, 249, 606, 1624
BstDEI CTNAG 9 cut(s) 101, 134, 194, 591, 998, 1160, 1208, 1590, 1715
BstEII GGTNACC 1 cut(s) 356
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 6 cut(s) 757, 779, 784, 1269, 2075, 2284
BstFNI CGCG 1 cut(s) 1419
BstH2I RGCGCY 1 cut(s) 2446
BstHHI GCGC 6 cut(s) 120, 1068, 1633, 1988, 2445, 2584
BstKTI GATC 7 cut(s) 259, 582, 1046, 1411, 1789, 2139, 2284
BstMAI GTCTC 4 cut(s) 192, 1106, 1361, 2651
BstMBI GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
BstMWI GCNNNNNNNGC 8 cut(s) 11, 433, 851, 1031, 1246, 1463, 1628, 2155
BstNI CCWGG 3 cut(s) 521, 1732, 1775
BstNSI RCATGY 1 cut(s) 2588
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 519, 1730, 1773
BstSFI CTRYAG 2 cut(s) 722, 1133
BstUI CGCG 1 cut(s) 1419
BstV1I GCAGC 8 cut(s) 287, 554, 828, 1039, 1373, 1620, 1995, 2161
BstV2I GAAGAC 2 cut(s) 947, 2136
BstX2I RGATCY 2 cut(s) 1043, 1786
BstYI RGATCY 2 cut(s) 1043, 1786
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 3 cut(s) 1412, 1720, 2217
BsuRI GGCC 1 cut(s) 1095
BtgZI GCGATG 1 cut(s) 1487
BtrI CACGTC 1 cut(s) 473
BtsCI GGATG 6 cut(s) 757, 779, 784, 1269, 2075, 2284
BtsI GCAGTG 2 cut(s) 794, 1523
BtsIMutI CAGTG 5 cut(s) 794, 1523, 2127, 2218, 2453
BveI ACCTGC 2 cut(s) 344, 2259
Cac8I GCNNGC 4 cut(s) 39, 249, 606, 1624
CaiI CAGNNNCTG 3 cut(s) 1589, 1774, 2155
CciI TCATGA 2 cut(s) 259, 2688
CfoI GCGC 6 cut(s) 120, 1068, 1633, 1988, 2445, 2584
Cfr10I RCCGGY 2 cut(s) 1622, 2705
Cfr13I GGNCC 3 cut(s) 370, 1225, 1915
Csp6I GTAC 5 cut(s) 1151, 1204, 1659, 2188, 2318
CspAI ACCGGT 1 cut(s) 2705
CviQI GTAC 5 cut(s) 1151, 1204, 1659, 2188, 2318
DdeI CTNAG 9 cut(s) 101, 134, 194, 591, 998, 1160, 1208, 1590, 1715
DpnI GATC 7 cut(s) 258, 581, 1045, 1410, 1788, 2138, 2283
DpnII GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
DraI TTTAAA 2 cut(s) 1668, 1707
DriI GACNNNNNGTC 1 cut(s) 1443
EaeI YGGCCR 1 cut(s) 1093
Eam1104I CTCTTC 6 cut(s) 150, 722, 1302, 1418, 2674, 2687
Eam1105I GACNNNNNGTC 1 cut(s) 1443
EarI CTCTTC 6 cut(s) 150, 722, 1302, 1418, 2674, 2687
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 172, 928
Eco130I CCWWGG 2 cut(s) 949, 1975
Eco24I GRGCYC 2 cut(s) 174, 930
Eco32I GATATC 1 cut(s) 326
Eco47I GGWCC 3 cut(s) 370, 1225, 1915
Eco53kI GAGCTC 2 cut(s) 172, 928
Eco57I CTGAAG 4 cut(s) 1751, 2103, 2162, 2282
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 1103
Eco91I GGTNACC 1 cut(s) 356
EcoICRI GAGCTC 2 cut(s) 172, 928
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 1915
EcoO65I GGTNACC 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 955
EcoRII CCWGG 3 cut(s) 519, 1730, 1773
EcoRV GATATC 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 949, 1975
EcoT22I ATGCAT 3 cut(s) 151, 2107, 2393
EcoT38I GRGCYC 2 cut(s) 174, 930
ErhI CCWWGG 2 cut(s) 949, 1975
FalI AAGNNNNNCTT 2 cut(s) 1648, 1680
FaqI GGGAC 2 cut(s) 2450, 2573
FauNDI CATATG 3 cut(s) 151, 1242, 1903
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 8 cut(s) 301, 543, 817, 1053, 1362, 1634, 1984, 2150
FokI GGATG 6 cut(s) 764, 786, 791, 1276, 2062, 2291
FriOI GRGCYC 2 cut(s) 174, 930
Fsp4HI GCNGC 8 cut(s) 301, 543, 817, 1053, 1362, 1634, 1984, 2150
FspBI CTAG 6 cut(s) 395, 605, 911, 2012, 2243, 2315
FspI TGCGCA 1 cut(s) 2583
GlaI GCGC 6 cut(s) 119, 1067, 1632, 1987, 2444, 2583
GluI GCNGC 8 cut(s) 301, 543, 817, 1053, 1362, 1634, 1984, 2150
GsaI CCCAGC 2 cut(s) 1622, 2006
GsuI CTGGAG 3 cut(s) 134, 897, 2477
HaeII RGCGCY 1 cut(s) 2446
HaeIII GGCC 1 cut(s) 1095
HapII CCGG 2 cut(s) 1623, 2706
HhaI GCGC 6 cut(s) 120, 1068, 1633, 1988, 2445, 2584
Hin6I GCGC 6 cut(s) 118, 1066, 1631, 1986, 2443, 2582
HinP1I GCGC 6 cut(s) 118, 1066, 1631, 1986, 2443, 2582
HindIII AAGCTT 3 cut(s) 35, 290, 1763
HinfI GANTC 7 cut(s) 341, 872, 917, 1354, 1693, 1889, 2539
HpaII CCGG 2 cut(s) 1623, 2706
HphI GGTGA 5 cut(s) 392, 893, 1420, 1981, 2596
Hpy166II GTNNAC 4 cut(s) 470, 1581, 1661, 1972
Hpy188III TCNNGA 9 cut(s) 133, 260, 307, 395, 876, 1283, 2324, 2536, 2689
Hpy8I GTNNAC 4 cut(s) 470, 1581, 1661, 1972
Hpy99I CGWCG 1 cut(s) 2339
HpyAV CCTTC 6 cut(s) 251, 1377, 1955, 2019, 2238, 2306
HpyCH4III ACNGT 3 cut(s) 992, 1100, 1585
HpyCH4IV ACGT 3 cut(s) 472, 571, 1564
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 433, 851, 1031, 1246, 1463, 1628, 2155
HpyF3I CTNAG 9 cut(s) 101, 134, 194, 591, 998, 1160, 1208, 1590, 1715
HpySE526I ACGT 3 cut(s) 472, 571, 1564
HspAI GCGC 6 cut(s) 118, 1066, 1631, 1986, 2443, 2582
KroI GCCGGC 1 cut(s) 1622
KroNI GCCGGC 1 cut(s) 1624
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
LguI GCTCTTC 1 cut(s) 2687
LmnI GCTCC 8 cut(s) 115, 169, 642, 925, 933, 1347, 1931, 2265
Lsp1109I GCAGC 8 cut(s) 287, 554, 828, 1039, 1373, 1620, 1995, 2161
LweI GCATC 9 cut(s) 106, 136, 154, 312, 373, 529, 769, 1221, 2378
MaeI CTAG 6 cut(s) 395, 605, 911, 2012, 2243, 2315
MaeII ACGT 3 cut(s) 472, 571, 1564
MaeIII GTNAC 5 cut(s) 356, 986, 1387, 1480, 2602
MalI GATC 7 cut(s) 258, 581, 1045, 1410, 1788, 2138, 2283
MboI GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
MflI RGATCY 2 cut(s) 1043, 1786
MhlI GDGCHC 2 cut(s) 174, 930
MlsI TGGCCA 1 cut(s) 1095
MluNI TGGCCA 1 cut(s) 1095
MlyI GAGTC 3 cut(s) 335, 926, 2533
MmeI TCCRAC 4 cut(s) 345, 1861, 2358, 2690
Mox20I TGGCCA 1 cut(s) 1095
Mph1103I ATGCAT 3 cut(s) 151, 2107, 2393
MroNI GCCGGC 1 cut(s) 1622
MroXI GAANNNNTTC 3 cut(s) 334, 560, 944
MscI TGGCCA 1 cut(s) 1095
MslI CAYNNNNRTG 8 cut(s) 213, 258, 378, 768, 1967, 2396, 2556, 2562
Msp20I TGGCCA 1 cut(s) 1095
MspA1I CMGCKG 2 cut(s) 697, 1376
MspCI CTTAAG 2 cut(s) 1790, 2504
MspI CCGG 2 cut(s) 1623, 2706
MspR9I CCNGG 3 cut(s) 521, 1732, 1775
Mva1269I GAATGC 2 cut(s) 1795, 2291
MvaI CCWGG 3 cut(s) 521, 1732, 1775
MvnI CGCG 1 cut(s) 1419
MwoI GCNNNNNNNGC 8 cut(s) 11, 433, 851, 1031, 1246, 1463, 1628, 2155
NaeI GCCGGC 1 cut(s) 1624
NdeI CATATG 3 cut(s) 151, 1242, 1903
NdeII GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
NgoMIV GCCGGC 1 cut(s) 1622
NheI GCTAGC 1 cut(s) 604
NlaIV GGNNCC 1 cut(s) 2704
NmuCI GTSAC 2 cut(s) 1387, 2602
NsbI TGCGCA 1 cut(s) 2583
NsiI ATGCAT 3 cut(s) 151, 2107, 2393
NspI RCATGY 1 cut(s) 2588
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 213
PagI TCATGA 2 cut(s) 259, 2688
PciSI GCTCTTC 1 cut(s) 2687
PctI GAATGC 2 cut(s) 1795, 2291
PdiI GCCGGC 1 cut(s) 1624
PdmI GAANNNNTTC 3 cut(s) 334, 560, 944
PfeI GAWTC 4 cut(s) 872, 1354, 1693, 1889
PinAI ACCGGT 1 cut(s) 2705
PkrI GCNGC 8 cut(s) 302, 544, 818, 1054, 1363, 1635, 1985, 2151
PleI GAGTC 3 cut(s) 335, 925, 2533
PpsI GAGTC 3 cut(s) 335, 925, 2533
Ppu21I YACGTR 1 cut(s) 572
PpuMI RGGWCCY 1 cut(s) 1915
Psp124BI GAGCTC 2 cut(s) 174, 930
Psp5II RGGWCCY 1 cut(s) 1915
Psp6I CCWGG 3 cut(s) 519, 1730, 1773
PspEI GGTNACC 1 cut(s) 356
PspFI CCCAGC 2 cut(s) 1618, 2002
PspGI CCWGG 3 cut(s) 519, 1730, 1773
PspN4I GGNNCC 1 cut(s) 2704
PspPI GGNCC 3 cut(s) 370, 1225, 1915
PspPPI RGGWCCY 1 cut(s) 1915
PstI CTGCAG 1 cut(s) 1137
PstNI CAGNNNCTG 3 cut(s) 1589, 1774, 2155
PsuI RGATCY 2 cut(s) 1043, 1786
PvuII CAGCTG 2 cut(s) 697, 1376
RsaI GTAC 5 cut(s) 1152, 1205, 1660, 2189, 2319
RsaNI GTAC 5 cut(s) 1151, 1204, 1659, 2188, 2318
RseI CAYNNNNRTG 8 cut(s) 213, 258, 378, 768, 1967, 2396, 2556, 2562
SacI GAGCTC 2 cut(s) 174, 930
SapI GCTCTTC 1 cut(s) 2687
SatI GCNGC 8 cut(s) 301, 543, 817, 1053, 1362, 1634, 1984, 2150
Sau3AI GATC 7 cut(s) 256, 579, 1043, 1408, 1786, 2136, 2281
Sau96I GGNCC 3 cut(s) 370, 1225, 1915
ScaI AGTACT 1 cut(s) 1152
SchI GAGTC 3 cut(s) 335, 926, 2533
ScrFI CCNGG 3 cut(s) 521, 1732, 1775
SduI GDGCHC 2 cut(s) 174, 930
SfaNI GCATC 9 cut(s) 106, 136, 154, 312, 373, 529, 769, 1221, 2378
SfcI CTRYAG 2 cut(s) 722, 1133
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 3 cut(s) 370, 1225, 1915
SmiMI CAYNNNNRTG 8 cut(s) 213, 258, 378, 768, 1967, 2396, 2556, 2562
SmlI CTYRAG 8 cut(s) 1720, 1790, 1800, 2324, 2351, 2504, 2611, 2650
SmoI CTYRAG 8 cut(s) 1720, 1790, 1800, 2324, 2351, 2504, 2611, 2650
SpeI ACTAGT 1 cut(s) 2011
SsiI CCGC 4 cut(s) 5, 167, 608, 1417
SspMI CTAG 6 cut(s) 395, 605, 911, 2012, 2243, 2315
SstI GAGCTC 2 cut(s) 174, 930
StyD4I CCNGG 3 cut(s) 519, 1730, 1773
StyI CCWWGG 2 cut(s) 949, 1975
TaaI ACNGT 3 cut(s) 992, 1100, 1585
TaiI ACGT 3 cut(s) 475, 574, 1567
TaqI TCGA 8 cut(s) 51, 322, 328, 376, 838, 1255, 2367, 2386
TatI WGTACW 4 cut(s) 1150, 1658, 2187, 2317
TfiI GAWTC 4 cut(s) 872, 1354, 1693, 1889
TscAI CASTG 5 cut(s) 801, 1530, 2127, 2225, 2460
TseFI GTSAC 2 cut(s) 1387, 2602
TseI GCWGC 8 cut(s) 300, 542, 816, 1052, 1361, 1633, 1983, 2149
Tsp45I GTSAC 2 cut(s) 1387, 2602
TspDTI ATGAA 9 cut(s) 168, 246, 698, 1267, 1347, 1951, 2064, 2394, 2705
TspGWI ACGGA 2 cut(s) 884, 2352
TspRI CASTG 5 cut(s) 801, 1530, 2127, 2225, 2460
Vha464I CTTAAG 2 cut(s) 1790, 2504
VpaK11BI GGWCC 3 cut(s) 370, 1225, 1915
XagI CCTNNNNNAGG 1 cut(s) 365
XapI RAATTY 5 cut(s) 90, 233, 955, 1394, 1824
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 2588
XcmI CCANNNNNNNNNTGG 1 cut(s) 2119
XmnI GAANNNNTTC 3 cut(s) 334, 560, 944
XspI CTAG 6 cut(s) 395, 605, 911, 2012, 2243, 2315
ZrmI AGTACT 1 cut(s) 1152
Zsp2I ATGCAT 3 cut(s) 151, 2107, 2393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.