Rroxscaffold_3G00247970

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000888
Physical Location & Seq
Reverse (-)
41299561 .. 41302911
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247970.1

Sequence Viewer

Length: 1668 bp
ATGGTTGGCCACAGTCTCGGGGCTGAAATTGAGGGCAATGACAAACTGCATGACTTGAAGAAAGTACTTTCACTCAGTTTCAATGATTTACCATACTATCTAAAATCGTGCTTCTTGTACCTGAGCATCTTTCCAGAGGACCATCTAATTAAGCATATGAGGCTTATTCGATTATGGATGGCAGAGGGCTTCATTGAAGAAAAACAAGGAAAGACATTGGAAGAGGTTGCAGAGGATTACCTCAATGAACTATTGAATAGGAGCATGATTCAAGCAGCAGAGACAACAGCTGATGGAAGGGTCACAAAATTTCGTATCCATGATCTCTTCCGTGAAATTATCATCTCCAAGACAAGAAATCAGAACTTTGCCACAATAGCCAAAGAGCAGAATGTAACATGGCCAGAAAAAATCCGTCGCCTGTCAATACATAACACACTGCAATATGTTCAGACAAACAGGTGTGCTTCCCAACTACGTTCTCTTTTTATGTTCACAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGCGCTGCTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCGGTTGAAGTTGTGAATCTTTACTTTTTGAAGTATCTGAGCTTGAGAGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCGAGTTACTGAACTCCCAGTTGAAATCCTGAAACTCCAACATCTGCGCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTTTTACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTTAAGATGAGAAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGATGAGATCATTGATTTGCAGCACCTGTGTTCTCCTCCTCTATTGCTTCAGCATTTGTACTTGCGAGGCAGATTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTTGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTACCCAATCTAGTACATCTTGAGTTGTGTCAGGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAGGAAGCTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCTTGTATTGAAAATCTAATCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGGTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCATTAGAGGGCTTGAGTGAGGCACAAAACTCTTCTCAACCCAGTTCTGTCATGAAGAGCATTGGAACTGGTTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

555

Amino Acids

64.25

Weight (kDa)

8.9

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 43 - 114 7.7e-27 Disease resistance protein Winged helix domain
LRR_14 PF23598 158 - 486 1.4e-46 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1533
Acc36I ACCTGC 1 cut(s) 1173
AciI CCGC 1 cut(s) 1287
AclWI GGATC 2 cut(s) 708, 1190
AcoI YGGCCR 2 cut(s) 7, 401
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 4 cut(s) 665, 1017, 1076, 1196
AfaI GTAC 5 cut(s) 66, 119, 574, 1103, 1233
AflII CTTAAG 2 cut(s) 704, 1454
AhlI ACTAGT 1 cut(s) 925
AjnI CCWGG 2 cut(s) 644, 687
AluBI AGCT 7 cut(s) 290, 633, 679, 848, 868, 916, 1300
AluI AGCT 7 cut(s) 290, 633, 679, 848, 868, 916, 1300
Alw26I GTCTC 3 cut(s) 20, 275, 1257
AlwI GGATC 2 cut(s) 708, 1190
AlwNI CAGNNNCTG 3 cut(s) 503, 688, 1069
Ama87I CYCGRG 2 cut(s) 17, 714
AoxI GGCC 2 cut(s) 7, 401
ApeKI GCWGC 4 cut(s) 275, 547, 897, 1063
ApoI RAATTY 1 cut(s) 308
AspLEI GCGC 5 cut(s) 547, 762, 902, 1395, 1534
AspS9I GGNCC 2 cut(s) 139, 829
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 895, 1546
AvaI CYCGRG 2 cut(s) 17, 714
AvaII GGWCC 2 cut(s) 139, 829
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 2 cut(s) 9, 403
BbvI GCAGC 4 cut(s) 287, 534, 909, 1075
BccI CCATC 7 cut(s) 150, 172, 287, 520, 965, 1342, 1426
BciT130I CCWGG 2 cut(s) 646, 689
BciVI GTATCC 2 cut(s) 326, 1131
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 3 cut(s) 20, 275, 1257
BcuI ACTAGT 1 cut(s) 925
BfaI CTAG 2 cut(s) 926, 1229
BfoI RGCGCY 1 cut(s) 1396
BfrI CTTAAG 2 cut(s) 704, 1454
BfuAI ACCTGC 1 cut(s) 1173
BfuI GTATCC 2 cut(s) 326, 1131
BisI GCNGC 4 cut(s) 276, 548, 898, 1064
BlsI GCNGC 4 cut(s) 277, 549, 899, 1065
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 3 cut(s) 593, 646, 689
Bme18I GGWCC 2 cut(s) 139, 829
BmeT110I CYCGRG 2 cut(s) 17, 714
BmgT120I GGNCC 2 cut(s) 139, 829
BmiI GGNNCC 1 cut(s) 1282
BmrFI CCNGG 3 cut(s) 593, 646, 689
BmrI ACTGGG 2 cut(s) 725, 1623
BmsI GCATC 2 cut(s) 135, 1328
BmuI ACTGGG 2 cut(s) 725, 1623
BpmI CTGGAG 1 cut(s) 1427
Bpu10I CCTNAGC 1 cut(s) 122
BpuEI CTTGAG 5 cut(s) 655, 1259, 1286, 1582, 1621
BpuMI CCSGG 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 645, 889
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bse118I RCCGGY 2 cut(s) 536, 1283
Bse1I ACTGG 5 cut(s) 731, 1139, 1410, 1629, 1660
Bse3DI GCAATG 2 cut(s) 43, 1519
BseBI CCWGG 2 cut(s) 646, 689
BseDI CCNNGG 2 cut(s) 645, 889
BseGI GGATG 4 cut(s) 183, 989, 1198, 1386
BseMI GCAATG 2 cut(s) 43, 1519
BseMII CTCAG 4 cut(s) 88, 113, 495, 620
BseNI ACTGG 5 cut(s) 731, 1139, 1410, 1629, 1660
BseRI GAGGAG 2 cut(s) 1068, 1071
BseXI GCAGC 4 cut(s) 287, 534, 909, 1075
BseYI CCCAGC 2 cut(s) 532, 916
BshFI GGCC 2 cut(s) 9, 403
BsiHKCI CYCGRG 2 cut(s) 17, 714
BsiSI CCGG 4 cut(s) 537, 593, 1284, 1390
BslFI GGGAC 2 cut(s) 1400, 1523
BsmAI GTCTC 3 cut(s) 20, 275, 1257
BsmFI GGGAC 2 cut(s) 1400, 1523
BsmI GAATGC 2 cut(s) 709, 1205
BsnI GGCC 2 cut(s) 9, 403
BsoBI CYCGRG 2 cut(s) 17, 714
Bsp143I GATC 4 cut(s) 322, 700, 1050, 1195
BspACI CCGC 1 cut(s) 1287
BspANI GGCC 2 cut(s) 9, 403
BspCNI CTCAG 4 cut(s) 87, 114, 496, 621
BspHI TCATGA 1 cut(s) 1638
BspLI GGNNCC 1 cut(s) 1282
BspMI ACCTGC 1 cut(s) 1173
BspPI GGATC 2 cut(s) 708, 1190
BspQI GCTCTTC 1 cut(s) 1637
BspTI CTTAAG 2 cut(s) 704, 1454
BsrDI GCAATG 2 cut(s) 43, 1519
BsrFI RCCGGY 2 cut(s) 536, 1283
BsrI ACTGG 5 cut(s) 731, 1139, 1410, 1629, 1660
BssAI RCCGGY 2 cut(s) 536, 1283
BssECI CCNNGG 2 cut(s) 645, 889
BssMI GATC 4 cut(s) 322, 700, 1050, 1195
BssT1I CCWWGG 1 cut(s) 889
Bst2UI CCWGG 2 cut(s) 646, 689
Bst4CI ACNGT 2 cut(s) 14, 499
Bst6I CTCTTC 4 cut(s) 216, 332, 1624, 1637
BstAFI CTTAAG 2 cut(s) 704, 1454
BstC8I GCNNGC 2 cut(s) 538, 1285
BstDEI CTNAG 4 cut(s) 74, 122, 504, 629
BstF5I GGATG 4 cut(s) 183, 989, 1198, 1386
BstH2I RGCGCY 1 cut(s) 1396
BstHHI GCGC 5 cut(s) 547, 762, 902, 1395, 1534
BstKTI GATC 4 cut(s) 325, 703, 1053, 1198
BstMAI GTCTC 3 cut(s) 20, 275, 1257
BstMBI GATC 4 cut(s) 322, 700, 1050, 1195
BstMWI GCNNNNNNNGC 3 cut(s) 160, 377, 542
BstNI CCWGG 2 cut(s) 646, 689
BstNSI RCATGY 1 cut(s) 1538
BstSCI CCNGG 3 cut(s) 591, 644, 687
BstV1I GCAGC 4 cut(s) 287, 534, 909, 1075
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BsuI GTATCC 2 cut(s) 326, 1131
BsuRI GGCC 2 cut(s) 9, 403
BtsCI GGATG 4 cut(s) 183, 989, 1198, 1386
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 4 cut(s) 437, 1041, 1132, 1403
BveI ACCTGC 1 cut(s) 1173
Cac8I GCNNGC 2 cut(s) 538, 1285
CaiI CAGNNNCTG 3 cut(s) 503, 688, 1069
CciI TCATGA 1 cut(s) 1638
CfoI GCGC 5 cut(s) 547, 762, 902, 1395, 1534
Cfr10I RCCGGY 2 cut(s) 536, 1283
Cfr13I GGNCC 2 cut(s) 139, 829
Csp6I GTAC 5 cut(s) 65, 118, 573, 1102, 1232
CviAII CATG 8 cut(s) 50, 265, 320, 399, 1502, 1508, 1535, 1639
CviQI GTAC 5 cut(s) 65, 118, 573, 1102, 1232
DdeI CTNAG 4 cut(s) 74, 122, 504, 629
DpnI GATC 4 cut(s) 324, 702, 1052, 1197
DpnII GATC 4 cut(s) 322, 700, 1050, 1195
DraI TTTAAA 1 cut(s) 582
EaeI YGGCCR 2 cut(s) 7, 401
Eam1104I CTCTTC 4 cut(s) 216, 332, 1624, 1637
EarI CTCTTC 4 cut(s) 216, 332, 1624, 1637
EciI GGCGGA 1 cut(s) 1302
Eco130I CCWWGG 1 cut(s) 889
Eco47I GGWCC 2 cut(s) 139, 829
Eco57I CTGAAG 4 cut(s) 665, 1017, 1076, 1196
Eco88I CYCGRG 2 cut(s) 17, 714
EcoO109I RGGNCCY 1 cut(s) 829
EcoRII CCWGG 2 cut(s) 644, 687
EcoT14I CCWWGG 1 cut(s) 889
EcoT22I ATGCAT 2 cut(s) 1021, 1343
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 8 cut(s) 53, 268, 323, 402, 1505, 1511, 1538, 1642
FalI AAGNNNNNCTT 2 cut(s) 562, 594
FaqI GGGAC 2 cut(s) 1400, 1523
FatI CATG 8 cut(s) 49, 264, 319, 398, 1501, 1507, 1534, 1638
FauNDI CATATG 2 cut(s) 156, 817
Fnu4HI GCNGC 4 cut(s) 276, 548, 898, 1064
FokI GGATG 4 cut(s) 190, 976, 1205, 1373
Fsp4HI GCNGC 4 cut(s) 276, 548, 898, 1064
FspBI CTAG 2 cut(s) 926, 1229
FspI TGCGCA 1 cut(s) 1533
GlaI GCGC 5 cut(s) 546, 761, 901, 1394, 1533
GluI GCNGC 4 cut(s) 276, 548, 898, 1064
GsaI CCCAGC 2 cut(s) 536, 920
GsuI CTGGAG 1 cut(s) 1427
HaeII RGCGCY 1 cut(s) 1396
HaeIII GGCC 2 cut(s) 9, 403
HapII CCGG 4 cut(s) 537, 593, 1284, 1390
HhaI GCGC 5 cut(s) 547, 762, 902, 1395, 1534
Hin1II CATG 8 cut(s) 53, 268, 323, 402, 1505, 1511, 1538, 1642
Hin6I GCGC 5 cut(s) 545, 760, 900, 1393, 1532
HinP1I GCGC 5 cut(s) 545, 760, 900, 1393, 1532
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 3 cut(s) 268, 607, 1489
HpaII CCGG 4 cut(s) 537, 593, 1284, 1390
HphI GGTGA 2 cut(s) 895, 1546
Hpy166II GTNNAC 3 cut(s) 495, 575, 886
Hpy188I TCNGA 5 cut(s) 363, 453, 630, 684, 1327
Hpy188III TCNNGA 6 cut(s) 134, 714, 742, 1238, 1486, 1639
Hpy8I GTNNAC 3 cut(s) 495, 575, 886
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 6 cut(s) 291, 869, 933, 1152, 1220, 1253
HpyCH4III ACNGT 2 cut(s) 14, 499
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 9 cut(s) 49, 230, 442, 568, 836, 1019, 1063, 1205, 1341
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 377, 542
HpyF3I CTNAG 4 cut(s) 74, 122, 504, 629
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 8 cut(s) 53, 268, 323, 402, 1505, 1511, 1538, 1642
HspAI GCGC 5 cut(s) 545, 760, 900, 1393, 1532
KroI GCCGGC 2 cut(s) 536, 1283
KroNI GCCGGC 2 cut(s) 538, 1285
Kzo9I GATC 4 cut(s) 322, 700, 1050, 1195
LguI GCTCTTC 1 cut(s) 1637
LmnI GCTCC 4 cut(s) 261, 845, 1179, 1280
Lsp1109I GCAGC 4 cut(s) 287, 534, 909, 1075
LweI GCATC 2 cut(s) 135, 1328
MaeI CTAG 2 cut(s) 926, 1229
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 4 cut(s) 301, 394, 718, 1552
MalI GATC 4 cut(s) 324, 702, 1052, 1197
MboI GATC 4 cut(s) 322, 700, 1050, 1195
MflI RGATCY 1 cut(s) 700
MlsI TGGCCA 2 cut(s) 9, 403
MluCI AATT 4 cut(s) 27, 147, 308, 336
MluNI TGGCCA 2 cut(s) 9, 403
MlyI GAGTC 1 cut(s) 1483
MmeI TCCRAC 2 cut(s) 775, 1640
Mox20I TGGCCA 2 cut(s) 9, 403
Mph1103I ATGCAT 2 cut(s) 1021, 1343
MroNI GCCGGC 2 cut(s) 536, 1283
MscI TGGCCA 2 cut(s) 9, 403
MseI TTAA 8 cut(s) 150, 552, 581, 705, 825, 954, 1187, 1455
MslI CAYNNNNRTG 4 cut(s) 881, 1346, 1506, 1512
Msp20I TGGCCA 2 cut(s) 9, 403
MspA1I CMGCKG 1 cut(s) 290
MspCI CTTAAG 2 cut(s) 704, 1454
MspI CCGG 4 cut(s) 537, 593, 1284, 1390
MspR9I CCNGG 3 cut(s) 593, 646, 689
Mva1269I GAATGC 2 cut(s) 709, 1205
MvaI CCWGG 2 cut(s) 646, 689
MwoI GCNNNNNNNGC 3 cut(s) 160, 377, 542
NaeI GCCGGC 2 cut(s) 538, 1285
NciI CCSGG 1 cut(s) 593
NdeI CATATG 2 cut(s) 156, 817
NdeII GATC 4 cut(s) 322, 700, 1050, 1195
NgoMIV GCCGGC 2 cut(s) 536, 1283
NlaIII CATG 8 cut(s) 53, 268, 323, 402, 1505, 1511, 1538, 1642
NlaIV GGNNCC 1 cut(s) 1282
NmuCI GTSAC 2 cut(s) 301, 1552
NsbI TGCGCA 1 cut(s) 1533
NsiI ATGCAT 2 cut(s) 1021, 1343
NspI RCATGY 1 cut(s) 1538
PaeR7I CTCGAG 1 cut(s) 714
PagI TCATGA 1 cut(s) 1638
PciSI GCTCTTC 1 cut(s) 1637
PctI GAATGC 2 cut(s) 709, 1205
PdiI GCCGGC 2 cut(s) 538, 1285
PfeI GAWTC 2 cut(s) 268, 607
PkrI GCNGC 4 cut(s) 277, 549, 899, 1065
PleI GAGTC 1 cut(s) 1483
PpsI GAGTC 1 cut(s) 1483
PpuMI RGGWCCY 1 cut(s) 829
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 2 cut(s) 644, 687
PspFI CCCAGC 2 cut(s) 532, 916
PspGI CCWGG 2 cut(s) 644, 687
PspN4I GGNNCC 1 cut(s) 1282
PspPI GGNCC 2 cut(s) 139, 829
PspPPI RGGWCCY 1 cut(s) 829
PstNI CAGNNNCTG 3 cut(s) 503, 688, 1069
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 5 cut(s) 66, 119, 574, 1103, 1233
RsaNI GTAC 5 cut(s) 65, 118, 573, 1102, 1232
RseI CAYNNNNRTG 4 cut(s) 881, 1346, 1506, 1512
SapI GCTCTTC 1 cut(s) 1637
SaqAI TTAA 8 cut(s) 150, 552, 581, 705, 825, 954, 1187, 1455
SatI GCNGC 4 cut(s) 276, 548, 898, 1064
Sau3AI GATC 4 cut(s) 322, 700, 1050, 1195
Sau96I GGNCC 2 cut(s) 139, 829
ScaI AGTACT 1 cut(s) 66
SchI GAGTC 1 cut(s) 1483
ScrFI CCNGG 3 cut(s) 593, 646, 689
SfaNI GCATC 2 cut(s) 135, 1328
Sfr274I CTCGAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 139, 829
SlaI CTCGAG 1 cut(s) 714
SmiMI CAYNNNNRTG 4 cut(s) 881, 1346, 1506, 1512
SmlI CTYRAG 8 cut(s) 634, 704, 714, 1238, 1301, 1454, 1561, 1600
SmoI CTYRAG 8 cut(s) 634, 704, 714, 1238, 1301, 1454, 1561, 1600
SpeI ACTAGT 1 cut(s) 925
Sse9I AATT 4 cut(s) 27, 147, 308, 336
SsiI CCGC 1 cut(s) 1287
SspMI CTAG 2 cut(s) 926, 1229
StyD4I CCNGG 3 cut(s) 591, 644, 687
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 2 cut(s) 14, 499
TaiI ACGT 1 cut(s) 481
TaqI TCGA 4 cut(s) 169, 715, 1317, 1336
TasI AATT 4 cut(s) 27, 147, 308, 336
TatI WGTACW 4 cut(s) 64, 572, 1101, 1231
TfiI GAWTC 2 cut(s) 268, 607
Tru1I TTAA 8 cut(s) 150, 552, 581, 705, 825, 954, 1187, 1455
Tru9I TTAA 8 cut(s) 150, 552, 581, 705, 825, 954, 1187, 1455
TscAI CASTG 4 cut(s) 444, 1041, 1139, 1410
TseFI GTSAC 2 cut(s) 301, 1552
TseI GCWGC 4 cut(s) 275, 547, 897, 1063
Tsp45I GTSAC 2 cut(s) 301, 1552
TspDTI ATGAA 6 cut(s) 181, 261, 865, 978, 1344, 1655
TspGWI ACGGA 2 cut(s) 320, 404
TspRI CASTG 4 cut(s) 444, 1041, 1139, 1410
Vha464I CTTAAG 2 cut(s) 704, 1454
VpaK11BI GGWCC 2 cut(s) 139, 829
XapI RAATTY 1 cut(s) 308
XceI RCATGY 1 cut(s) 1538
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XhoI CTCGAG 1 cut(s) 714
XspI CTAG 2 cut(s) 926, 1229
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 2 cut(s) 1021, 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.