Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2147 |
| Acc16I | TGCGCA | 1 cut(s) | 1590 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1963 |
| AciI | CCGC | 3 cut(s) | 1198, 1519, 2290 |
| AclWI | GGATC | 9 cut(s) | 638, 690, 1035, 1496, 1498, 1511, 1927, 1980, 2384 |
| AcsI | RAATTY | 3 cut(s) | 245, 416, 1781 |
| AcuI | CTGAAG | 7 cut(s) | 249, 450, 1859, 2211, 2270, 2516, 2751 |
| AcyI | GRCGYC | 1 cut(s) | 1612 |
| AfaI | GTAC | 5 cut(s) | 63, 1307, 1717, 1808, 2297 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 147, 1363, 1963, 2036 |
| AflII | CTTAAG | 2 cut(s) | 1898, 2503 |
| AflIII | ACRYGT | 1 cut(s) | 1496 |
| AhlI | ACTAGT | 1 cut(s) | 1472 |
| AjnI | CCWGG | 1 cut(s) | 2508 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 605, 637, 2294, 2326, 2692, 2724 |
| Alw21I | GWGCWC | 1 cut(s) | 1631 |
| Alw26I | GTCTC | 5 cut(s) | 397, 751, 2367, 2451, 2722 |
| AlwI | GGATC | 9 cut(s) | 638, 690, 1035, 1496, 1498, 1511, 1927, 1980, 2384 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2293 |
| Ama87I | CYCGRG | 2 cut(s) | 2111, 2648 |
| AoxI | GGCC | 2 cut(s) | 138, 2140 |
| ApeKI | GCWGC | 4 cut(s) | 820, 918, 1200, 1741 |
| ApoI | RAATTY | 3 cut(s) | 245, 416, 1781 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 234, 729, 1474 |
| AspLEI | GCGC | 3 cut(s) | 576, 1591, 2215 |
| AspS9I | GGNCC | 1 cut(s) | 138 |
| AsuHPI | GGTGA | 3 cut(s) | 1477, 1549, 1855 |
| AvaI | CYCGRG | 2 cut(s) | 2111, 2648 |
| AxyI | CCTNAGG | 2 cut(s) | 146, 1323 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 53, 86 |
| BamHI | GGATCC | 1 cut(s) | 1503 |
| BbsI | GAAGAC | 2 cut(s) | 620, 2687 |
| Bbv12I | GWGCWC | 1 cut(s) | 1631 |
| BbvI | GCAGC | 4 cut(s) | 807, 930, 1212, 1728 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 458, 492 |
| BciT130I | CCWGG | 1 cut(s) | 2510 |
| BciVI | GTATCC | 2 cut(s) | 874, 1672 |
| BclI | TGATCA | 1 cut(s) | 268 |
| BcoDI | GTCTC | 5 cut(s) | 397, 751, 2367, 2451, 2722 |
| BcuI | ACTAGT | 1 cut(s) | 1472 |
| BfaI | CTAG | 8 cut(s) | 29, 239, 497, 1473, 1883, 1892, 2177, 2351 |
| BfmI | CTRYAG | 3 cut(s) | 153, 298, 1014 |
| BfrI | CTTAAG | 2 cut(s) | 1898, 2503 |
| BfuI | GTATCC | 2 cut(s) | 874, 1672 |
| BglII | AGATCT | 2 cut(s) | 1894, 2499 |
| BisI | GCNGC | 5 cut(s) | 821, 919, 1201, 1742, 2291 |
| BlsI | GCNGC | 5 cut(s) | 822, 920, 1202, 1743, 2292 |
| Bme1390I | CCNGG | 1 cut(s) | 2510 |
| BmeT110I | CYCGRG | 2 cut(s) | 2111, 2648 |
| BmgT120I | GGNCC | 1 cut(s) | 138 |
| BmiI | GGNNCC | 1 cut(s) | 1505 |
| BmrFI | CCNGG | 1 cut(s) | 2510 |
| BmrI | ACTGGG | 1 cut(s) | 346 |
| BmsI | GCATC | 6 cut(s) | 148, 475, 874, 1323, 1993, 2635 |
| BmuI | ACTGGG | 1 cut(s) | 346 |
| BpiI | GAAGAC | 2 cut(s) | 620, 2687 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 702, 734, 915, 947 |
| BpmI | CTGGAG | 2 cut(s) | 729, 2621 |
| Bpu10I | CCTNAGC | 3 cut(s) | 824, 1310, 2381 |
| BpuEI | CTTGAG | 5 cut(s) | 355, 1220, 1843, 1849, 1922 |
| Bsa29I | ATCGAT | 1 cut(s) | 694 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1425, 1529 |
| BsaHI | GRCGYC | 1 cut(s) | 1612 |
| BsaJI | CCNNGG | 4 cut(s) | 588, 621, 1204, 1839 |
| BsaWI | WCCGGW | 2 cut(s) | 1356, 2453 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 147, 1363, 1963, 2036 |
| Bse1I | ACTGG | 5 cut(s) | 352, 1787, 2333, 2666, 2753 |
| Bse21I | CCTNAGG | 2 cut(s) | 146, 1323 |
| Bse3DI | GCAATG | 4 cut(s) | 161, 1598, 2051, 2562 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1425, 1529 |
| BseBI | CCWGG | 1 cut(s) | 2510 |
| BseCI | ATCGAT | 1 cut(s) | 694 |
| BseDI | CCNNGG | 4 cut(s) | 588, 621, 1204, 1839 |
| BseGI | GGATG | 8 cut(s) | 283, 859, 1620, 1710, 2170, 2183, 2392, 2580 |
| BseJI | GATNNNNATC | 2 cut(s) | 1425, 1529 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 147, 1363, 1963, 2036 |
| BseMI | GCAATG | 4 cut(s) | 161, 1598, 2051, 2562 |
| BseMII | CTCAG | 7 cut(s) | 137, 1276, 1301, 1314, 2120, 2189, 2357 |
| BseNI | ACTGG | 5 cut(s) | 352, 1787, 2333, 2666, 2753 |
| BseRI | GAGGAG | 3 cut(s) | 495, 1059, 2262 |
| BseXI | GCAGC | 4 cut(s) | 807, 930, 1212, 1728 |
| BshFI | GGCC | 2 cut(s) | 140, 2142 |
| BshVI | ATCGAT | 1 cut(s) | 694 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1631 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2111, 2648 |
| BsiSI | CCGG | 2 cut(s) | 1357, 2454 |
| BslFI | GGGAC | 1 cut(s) | 2711 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 147, 1363, 1963, 2036 |
| BsmAI | GTCTC | 5 cut(s) | 397, 751, 2367, 2451, 2722 |
| BsmBI | CGTCTC | 1 cut(s) | 751 |
| BsmFI | GGGAC | 1 cut(s) | 2711 |
| BsmI | GAATGC | 3 cut(s) | 328, 519, 1903 |
| BsnI | GGCC | 2 cut(s) | 140, 2142 |
| BsoBI | CYCGRG | 2 cut(s) | 2111, 2648 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1631 |
| Bsp1407I | TGTACA | 1 cut(s) | 2295 |
| BspACI | CCGC | 3 cut(s) | 1198, 1519, 2290 |
| BspANI | GGCC | 2 cut(s) | 140, 2142 |
| BspCNI | CTCAG | 7 cut(s) | 138, 1275, 1302, 1315, 2121, 2190, 2358 |
| BspDI | ATCGAT | 1 cut(s) | 694 |
| BspHI | TCATGA | 2 cut(s) | 220, 523 |
| BspLI | GGNNCC | 1 cut(s) | 1505 |
| BspMAI | CTGCAG | 2 cut(s) | 157, 302 |
| BspPI | GGATC | 9 cut(s) | 638, 690, 1035, 1496, 1498, 1511, 1927, 1980, 2384 |
| BspQI | GCTCTTC | 1 cut(s) | 495 |
| BspTI | CTTAAG | 2 cut(s) | 1898, 2503 |
| BsrDI | GCAATG | 4 cut(s) | 161, 1598, 2051, 2562 |
| BsrGI | TGTACA | 1 cut(s) | 2295 |
| BsrI | ACTGG | 5 cut(s) | 352, 1787, 2333, 2666, 2753 |
| BssECI | CCNNGG | 4 cut(s) | 588, 621, 1204, 1839 |
| BssNI | GRCGYC | 1 cut(s) | 1612 |
| BssT1I | CCWWGG | 4 cut(s) | 588, 621, 1204, 1839 |
| Bst2UI | CCWGG | 1 cut(s) | 2510 |
| Bst4CI | ACNGT | 8 cut(s) | 452, 476, 688, 1564, 1642, 1680, 1852, 2589 |
| Bst6I | CTCTTC | 4 cut(s) | 495, 746, 2346, 2695 |
| BstACI | GRCGYC | 1 cut(s) | 1612 |
| BstAFI | CTTAAG | 2 cut(s) | 1898, 2503 |
| BstAUI | TGTACA | 1 cut(s) | 2295 |
| BstC8I | GCNNGC | 3 cut(s) | 122, 675, 1198 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 8 cut(s) | 283, 859, 1620, 1710, 2170, 2183, 2392, 2580 |
| BstHHI | GCGC | 3 cut(s) | 576, 1591, 2215 |
| BstMAI | GTCTC | 5 cut(s) | 397, 751, 2367, 2451, 2722 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 224, 918, 953, 2125, 2290 |
| BstNI | CCWGG | 1 cut(s) | 2510 |
| BstNSI | RCATGY | 3 cut(s) | 896, 1500, 2726 |
| BstSCI | CCNGG | 1 cut(s) | 2508 |
| BstSFI | CTRYAG | 3 cut(s) | 153, 298, 1014 |
| BstV1I | GCAGC | 4 cut(s) | 807, 930, 1212, 1728 |
| BstV2I | GAAGAC | 2 cut(s) | 620, 2687 |
| BstX2I | RGATCY | 6 cut(s) | 1027, 1503, 1894, 1932, 1985, 2499 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2090 |
| BstYI | RGATCY | 6 cut(s) | 1027, 1503, 1894, 1932, 1985, 2499 |
| Bsu15I | ATCGAT | 1 cut(s) | 694 |
| Bsu36I | CCTNAGG | 2 cut(s) | 146, 1323 |
| BsuI | GTATCC | 2 cut(s) | 874, 1672 |
| BsuRI | GGCC | 2 cut(s) | 140, 2142 |
| BsuTUI | ATCGAT | 1 cut(s) | 694 |
| BtsCI | GGATG | 8 cut(s) | 283, 859, 1620, 1710, 2170, 2183, 2392, 2580 |
| BtsI | GCAGTG | 1 cut(s) | 896 |
| BtsIMutI | CAGTG | 7 cut(s) | 457, 684, 896, 1676, 2235, 2326, 2673 |
| Cac8I | GCNNGC | 3 cut(s) | 122, 675, 1198 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2293 |
| CciI | TCATGA | 2 cut(s) | 220, 523 |
| CfoI | GCGC | 3 cut(s) | 576, 1591, 2215 |
| Cfr13I | GGNCC | 1 cut(s) | 138 |
| ClaI | ATCGAT | 1 cut(s) | 694 |
| CseI | GACGC | 1 cut(s) | 1620 |
| Csp6I | GTAC | 5 cut(s) | 62, 1306, 1716, 1807, 2296 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 895, 930 |
| CviQI | GTAC | 5 cut(s) | 62, 1306, 1716, 1807, 2296 |
| DraI | TTTAAA | 2 cut(s) | 559, 1101 |
| Eam1104I | CTCTTC | 4 cut(s) | 495, 746, 2346, 2695 |
| EarI | CTCTTC | 4 cut(s) | 495, 746, 2346, 2695 |
| Eco130I | CCWWGG | 4 cut(s) | 588, 621, 1204, 1839 |
| Eco147I | AGGCCT | 1 cut(s) | 2142 |
| Eco57I | CTGAAG | 7 cut(s) | 249, 450, 1859, 2211, 2270, 2516, 2751 |
| Eco81I | CCTNAGG | 2 cut(s) | 146, 1323 |
| Eco88I | CYCGRG | 2 cut(s) | 2111, 2648 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 145 |
| EcoO109I | RGGNCCY | 1 cut(s) | 138 |
| EcoRII | CCWGG | 1 cut(s) | 2508 |
| EcoT14I | CCWWGG | 4 cut(s) | 588, 621, 1204, 1839 |
| EcoT22I | ATGCAT | 1 cut(s) | 163 |
| ErhI | CCWWGG | 4 cut(s) | 588, 621, 1204, 1839 |
| Esp3I | CGTCTC | 1 cut(s) | 751 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 605, 637 |
| FaqI | GGGAC | 1 cut(s) | 2711 |
| FauI | CCCGC | 2 cut(s) | 1205, 1512 |
| FbaI | TGATCA | 1 cut(s) | 268 |
| Fnu4HI | GCNGC | 5 cut(s) | 821, 919, 1201, 1742, 2291 |
| FokI | GGATG | 8 cut(s) | 290, 866, 1607, 1697, 2170, 2177, 2399, 2567 |
| Fsp4HI | GCNGC | 5 cut(s) | 821, 919, 1201, 1742, 2291 |
| FspBI | CTAG | 8 cut(s) | 29, 239, 497, 1473, 1883, 1892, 2177, 2351 |
| FspI | TGCGCA | 1 cut(s) | 1590 |
| GlaI | GCGC | 3 cut(s) | 575, 1590, 2214 |
| GluI | GCNGC | 5 cut(s) | 821, 919, 1201, 1742, 2291 |
| GsuI | CTGGAG | 2 cut(s) | 729, 2621 |
| HaeIII | GGCC | 2 cut(s) | 140, 2142 |
| HapII | CCGG | 2 cut(s) | 1357, 2454 |
| HgaI | GACGC | 1 cut(s) | 1620 |
| HhaI | GCGC | 3 cut(s) | 576, 1591, 2215 |
| Hin1I | GRCGYC | 1 cut(s) | 1612 |
| Hin6I | GCGC | 3 cut(s) | 574, 1589, 2213 |
| HinP1I | GCGC | 3 cut(s) | 574, 1589, 2213 |
| HindIII | AAGCTT | 4 cut(s) | 330, 1871, 1940, 2594 |
| HinfI | GANTC | 8 cut(s) | 282, 375, 405, 1019, 1860, 1879, 2332, 2715 |
| HpaII | CCGG | 2 cut(s) | 1357, 2454 |
| HphI | GGTGA | 3 cut(s) | 1477, 1549, 1855 |
| Hpy166II | GTNNAC | 4 cut(s) | 64, 1801, 1919, 2045 |
| Hpy8I | GTNNAC | 4 cut(s) | 64, 1801, 1919, 2045 |
| Hpy99I | CGWCG | 2 cut(s) | 480, 1614 |
| HpyAV | CCTTC | 8 cut(s) | 151, 428, 828, 1367, 1479, 2127, 2164, 2346 |
| HpyCH4III | ACNGT | 8 cut(s) | 452, 476, 688, 1564, 1642, 1680, 1852, 2589 |
| HpyCH4IV | ACGT | 2 cut(s) | 2308, 2541 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 224, 918, 953, 2125, 2290 |
| HpySE526I | ACGT | 2 cut(s) | 2308, 2541 |
| Hsp92I | GRCGYC | 1 cut(s) | 1612 |
| HspAI | GCGC | 3 cut(s) | 574, 1589, 2213 |
| Ksp22I | TGATCA | 1 cut(s) | 268 |
| LguI | GCTCTTC | 1 cut(s) | 495 |
| LmnI | GCTCC | 2 cut(s) | 261, 817 |
| Lsp1109I | GCAGC | 4 cut(s) | 807, 930, 1212, 1728 |
| LweI | GCATC | 6 cut(s) | 148, 475, 874, 1323, 1993, 2635 |
| MaeI | CTAG | 8 cut(s) | 29, 239, 497, 1473, 1883, 1892, 2177, 2351 |
| MaeII | ACGT | 2 cut(s) | 2308, 2541 |
| MaeIII | GTNAC | 4 cut(s) | 682, 1132, 1257, 2023 |
| MflI | RGATCY | 6 cut(s) | 1027, 1503, 1894, 1932, 1985, 2499 |
| MhlI | GDGCHC | 1 cut(s) | 1631 |
| MlyI | GAGTC | 4 cut(s) | 384, 399, 1028, 2724 |
| MmeI | TCCRAC | 6 cut(s) | 433, 1481, 1633, 2431, 2608, 2713 |
| Mph1103I | ATGCAT | 1 cut(s) | 163 |
| MroXI | GAANNNNTTC | 3 cut(s) | 234, 729, 1474 |
| MslI | CAYNNNNRTG | 5 cut(s) | 264, 270, 276, 870, 1908 |
| MspA1I | CMGCKG | 3 cut(s) | 912, 2128, 2290 |
| MspCI | CTTAAG | 2 cut(s) | 1898, 2503 |
| MspI | CCGG | 2 cut(s) | 1357, 2454 |
| MspR9I | CCNGG | 1 cut(s) | 2510 |
| Mva1269I | GAATGC | 3 cut(s) | 328, 519, 1903 |
| MvaI | CCWGG | 1 cut(s) | 2510 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 224, 918, 953, 2125, 2290 |
| NlaIV | GGNNCC | 1 cut(s) | 1505 |
| NmeAIII | GCCGAG | 2 cut(s) | 947, 1881 |
| NmuCI | GTSAC | 3 cut(s) | 682, 1132, 2023 |
| NsbI | TGCGCA | 1 cut(s) | 1590 |
| NsiI | ATGCAT | 1 cut(s) | 163 |
| NspI | RCATGY | 3 cut(s) | 896, 1500, 2726 |
| PaeR7I | CTCGAG | 1 cut(s) | 2648 |
| PagI | TCATGA | 2 cut(s) | 220, 523 |
| PceI | AGGCCT | 1 cut(s) | 2142 |
| PciI | ACATGT | 1 cut(s) | 1496 |
| PciSI | GCTCTTC | 1 cut(s) | 495 |
| PctI | GAATGC | 3 cut(s) | 328, 519, 1903 |
| PdmI | GAANNNNTTC | 3 cut(s) | 234, 729, 1474 |
| PfeI | GAWTC | 4 cut(s) | 282, 1860, 1879, 2332 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1963 |
| PkrI | GCNGC | 5 cut(s) | 822, 920, 1202, 1743, 2292 |
| PleI | GAGTC | 4 cut(s) | 383, 399, 1027, 2723 |
| PpsI | GAGTC | 4 cut(s) | 383, 399, 1027, 2723 |
| PscI | ACATGT | 1 cut(s) | 1496 |
| PsiI | TTATAA | 1 cut(s) | 2147 |
| Psp6I | CCWGG | 1 cut(s) | 2508 |
| PspGI | CCWGG | 1 cut(s) | 2508 |
| PspN4I | GGNNCC | 1 cut(s) | 1505 |
| PspPI | GGNCC | 1 cut(s) | 138 |
| PstI | CTGCAG | 2 cut(s) | 157, 302 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2293 |
| PsuI | RGATCY | 6 cut(s) | 1027, 1503, 1894, 1932, 1985, 2499 |
| PvuII | CAGCTG | 2 cut(s) | 912, 2128 |
| RsaI | GTAC | 5 cut(s) | 63, 1307, 1717, 1808, 2297 |
| RsaNI | GTAC | 5 cut(s) | 62, 1306, 1716, 1807, 2296 |
| RseI | CAYNNNNRTG | 5 cut(s) | 264, 270, 276, 870, 1908 |
| SapI | GCTCTTC | 1 cut(s) | 495 |
| SatI | GCNGC | 5 cut(s) | 821, 919, 1201, 1742, 2291 |
| Sau96I | GGNCC | 1 cut(s) | 138 |
| SchI | GAGTC | 4 cut(s) | 384, 399, 1028, 2724 |
| ScrFI | CCNGG | 1 cut(s) | 2510 |
| SduI | GDGCHC | 1 cut(s) | 1631 |
| SfaNI | GCATC | 6 cut(s) | 148, 475, 874, 1323, 1993, 2635 |
| SfcI | CTRYAG | 3 cut(s) | 153, 298, 1014 |
| Sfr274I | CTCGAG | 1 cut(s) | 2648 |
| SlaI | CTCGAG | 1 cut(s) | 2648 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 264, 270, 276, 870, 1908 |
| SmlI | CTYRAG | 8 cut(s) | 370, 1235, 1822, 1828, 1898, 1937, 2503, 2648 |
| SmoI | CTYRAG | 8 cut(s) | 370, 1235, 1822, 1828, 1898, 1937, 2503, 2648 |
| SpeI | ACTAGT | 1 cut(s) | 1472 |
| SseBI | AGGCCT | 1 cut(s) | 2142 |
| SsiI | CCGC | 3 cut(s) | 1198, 1519, 2290 |
| SspMI | CTAG | 8 cut(s) | 29, 239, 497, 1473, 1883, 1892, 2177, 2351 |
| StuI | AGGCCT | 1 cut(s) | 2142 |
| StyD4I | CCNGG | 1 cut(s) | 2508 |
| StyI | CCWWGG | 4 cut(s) | 588, 621, 1204, 1839 |
| TaaI | ACNGT | 8 cut(s) | 452, 476, 688, 1564, 1642, 1680, 1852, 2589 |
| TaiI | ACGT | 2 cut(s) | 2311, 2544 |
| TaqI | TCGA | 6 cut(s) | 115, 478, 694, 2443, 2520, 2649 |
| TatI | WGTACW | 2 cut(s) | 1806, 2295 |
| TauI | GCSGC | 1 cut(s) | 2293 |
| TfiI | GAWTC | 4 cut(s) | 282, 1860, 1879, 2332 |
| TscAI | CASTG | 7 cut(s) | 457, 691, 903, 1683, 2235, 2333, 2673 |
| TseFI | GTSAC | 3 cut(s) | 682, 1132, 2023 |
| TseI | GCWGC | 4 cut(s) | 820, 918, 1200, 1741 |
| Tsp45I | GTSAC | 3 cut(s) | 682, 1132, 2023 |
| TspGWI | ACGGA | 3 cut(s) | 1703, 1904, 2319 |
| TspRI | CASTG | 7 cut(s) | 457, 691, 903, 1683, 2235, 2333, 2673 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1963 |
| Vha464I | CTTAAG | 2 cut(s) | 1898, 2503 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 145 |
| XapI | RAATTY | 3 cut(s) | 245, 416, 1781 |
| XbaI | TCTAGA | 4 cut(s) | 238, 496, 1882, 1891 |
| XceI | RCATGY | 3 cut(s) | 896, 1500, 2726 |
| XhoI | CTCGAG | 1 cut(s) | 2648 |
| XmnI | GAANNNNTTC | 3 cut(s) | 234, 729, 1474 |
| XspI | CTAG | 8 cut(s) | 29, 239, 497, 1473, 1883, 1892, 2177, 2351 |
| Zsp2I | ATGCAT | 1 cut(s) | 163 |