Rh2AG385400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000888
Physical Location & Seq
Reverse (-)
57540084 .. 57566814
26731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG385400.1

Sequence Viewer

Length: 2016 bp
ATGGCGGAGAGTGCGGTAAAGTTTCTACTTGACAAGCTTGCACCTCTTTTCGAAAATGAGTTGCAACTTTTGAGAGGGGTTAGGGAAGAAATTTTGTATCTAAGAGGAGAACTGGAGCGCATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAACTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCAGAGTTACGAGCCATAAACTCCAGGATCAAAAAAATCTCTGAGGTTCATAAGCGACTCCGCCACAAATTCAATATGGCTGAACAAGGCTCAAGCTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAGGTGATGCTTTACTTCTAGAGAAAACTGATATAGTGGGCATAGACGAGCCTATTAAGCAGATGATTGGGAGGCTGGTCAATGGTGGTTGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGGGAAAACTACCCTGGCGAAGCAAGTCTATGATGCAACAGAAGTGAAGAAACATTTCAAATTACGTGCTTGGATCACTTTTACTCAGTCTTTCAGGCTAGCGGAACTTCTCAAAGACATCGTTCAACAGCTCCACCAAGCAATCCGAAGTCCAGTTCCACAAGAAATCAATAGCATGAATACCAACCAGCTGAAAACAATAATTAAAGACTTCCTACAGAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCGAGTTACTGAACTCCCAGTTGAAATCCTGAAACTCCAACATCTTCGCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTTTCACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAAAAGGTTAGGCATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGCAGCACCTGTGTTCTCCTCCTCTGTTGCTTCAGCGTTTGTACTTGCGAGGCAGATTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTCGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAATATTTACCCAACCTAGTACATCTTGAGTTGTGTCAAGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAAGAAGCTTAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCCTATATTGAAAATCTAAGCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGGTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCACTAGAGGGCTTGAGTGAGACACAAAACTCTTCTCAACCCAGTTCTGTCATGAAGAGCCTTGGAACTGATTGGAAGTTATTCATGTATGAAGCAGTGTTGGCAATTTGTGCTAGTCAGATAATTAAAGTCAATGCCTTGTGCTCTAAGACTTGCACATTTCAAGTTGAGGTAGATATGAGAGTGCTAGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

671

Amino Acids

77.05

Weight (kDa)

8.76

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 1.1e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 294 3.2e-28 NB-ARC domain
AAA_16 PF13191 177 - 283 3.1e-06 AAA ATPase domain
LRR_14 PF23598 292 - 563 1.3e-37 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1764
Acc36I ACCTGC 2 cut(s) 434, 1404
AciI CCGC 6 cut(s) 5, 14, 167, 397, 698, 1518
AclWI GGATC 4 cut(s) 371, 677, 939, 1421
AcsI RAATTY 3 cut(s) 90, 233, 404
AcuI CTGAAG 4 cut(s) 321, 896, 1248, 1307
AfaI GTAC 2 cut(s) 1334, 1464
AfiI CCNNNNNNNGG 1 cut(s) 457
AflII CTTAAG 3 cut(s) 935, 1532, 1685
AhlI ACTAGT 1 cut(s) 1156
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 3 cut(s) 359, 609, 918
AleI CACNNNNGTG 1 cut(s) 213
Alw21I GWGCWC 1 cut(s) 1964
Alw26I GTCTC 3 cut(s) 192, 1488, 1832
AlwI GGATC 4 cut(s) 371, 677, 939, 1421
AlwNI CAGNNNCTG 2 cut(s) 919, 1300
Ama87I CYCGRG 1 cut(s) 945
ApeKI GCWGC 3 cut(s) 300, 1128, 1294
ApoI RAATTY 3 cut(s) 90, 233, 404
Asp700I GAANNNNTTC 5 cut(s) 222, 334, 650, 1035, 1898
AspLEI GCGC 4 cut(s) 120, 1133, 1626, 1765
AspS9I GGNCC 2 cut(s) 460, 1060
AsuHPI GGTGA 3 cut(s) 482, 1126, 1777
AsuII TTCGAA 1 cut(s) 51
AsuNHI GCTAGC 1 cut(s) 694
AvaI CYCGRG 1 cut(s) 945
AvaII GGWCC 2 cut(s) 460, 1060
AxyI CCTNAGG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 1281
Bbv12I GWGCWC 1 cut(s) 1964
BbvI GCAGC 3 cut(s) 287, 1140, 1306
BccI CCATC 5 cut(s) 471, 858, 1196, 1573, 1657
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 3 cut(s) 361, 611, 920
BciVI GTATCC 1 cut(s) 1362
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 192, 1488, 1832
BcuI ACTAGT 1 cut(s) 1156
BfaI CTAG 7 cut(s) 485, 695, 1157, 1460, 1823, 1932, 2006
BfmI CTRYAG 1 cut(s) 812
BfoI RGCGCY 1 cut(s) 1627
BfrI CTTAAG 3 cut(s) 935, 1532, 1685
BfuAI ACCTGC 2 cut(s) 434, 1404
BfuI GTATCC 1 cut(s) 1362
BisI GCNGC 3 cut(s) 301, 1129, 1295
BlsI GCNGC 3 cut(s) 302, 1130, 1296
Bme1390I CCNGG 3 cut(s) 361, 611, 920
Bme18I GGWCC 2 cut(s) 460, 1060
BmeT110I CYCGRG 1 cut(s) 945
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 460, 1060
BmiI GGNNCC 1 cut(s) 1513
BmrFI CCNGG 3 cut(s) 361, 611, 920
BmrI ACTGGG 2 cut(s) 956, 1854
BmsI GCATC 8 cut(s) 136, 154, 312, 463, 619, 859, 1559, 1626
BmtI GCTAGC 1 cut(s) 698
BmuI ACTGGG 2 cut(s) 956, 1854
BpiI GAAGAC 1 cut(s) 1281
BpmI CTGGAG 3 cut(s) 134, 343, 1658
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 4 cut(s) 412, 1490, 1813, 1852
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 609, 1120, 1879
BsaXI ACNNNNNCTCC 2 cut(s) 1362, 1392
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse118I RCCGGY 1 cut(s) 1514
Bse1I ACTGG 6 cut(s) 117, 749, 962, 1370, 1641, 1860
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 2 cut(s) 201, 1750
BseBI CCWGG 3 cut(s) 361, 611, 920
BseDI CCNNGG 3 cut(s) 609, 1120, 1879
BseGI GGATG 6 cut(s) 847, 869, 874, 1220, 1429, 1617
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 2 cut(s) 201, 1750
BseMII CTCAG 3 cut(s) 125, 369, 695
BseNI ACTGG 6 cut(s) 117, 749, 962, 1370, 1641, 1860
BseRI GAGGAG 4 cut(s) 120, 835, 1299, 1302
BseXI GCAGC 3 cut(s) 287, 1140, 1306
BseYI CCCAGC 1 cut(s) 1147
BsgI GTGCAG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 1964
BsiHKCI CYCGRG 1 cut(s) 945
BsiSI CCGG 2 cut(s) 1515, 1621
BslFI GGGAC 2 cut(s) 1631, 1754
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 3 cut(s) 192, 1488, 1832
BsmFI GGGAC 2 cut(s) 1631, 1754
BsmI GAATGC 2 cut(s) 940, 1436
BsoBI CYCGRG 1 cut(s) 945
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 1964
Bsp143I GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
BspACI CCGC 6 cut(s) 5, 14, 167, 397, 698, 1518
BspCNI CTCAG 3 cut(s) 126, 370, 694
BspHI TCATGA 1 cut(s) 1869
BspLI GGNNCC 1 cut(s) 1513
BspMI ACCTGC 2 cut(s) 434, 1404
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 4 cut(s) 371, 677, 939, 1421
BspQI GCTCTTC 1 cut(s) 1868
BspT104I TTCGAA 1 cut(s) 51
BspTI CTTAAG 3 cut(s) 935, 1532, 1685
BsrDI GCAATG 2 cut(s) 201, 1750
BsrFI RCCGGY 1 cut(s) 1514
BsrI ACTGG 6 cut(s) 117, 749, 962, 1370, 1641, 1860
BssAI RCCGGY 1 cut(s) 1514
BssECI CCNNGG 3 cut(s) 609, 1120, 1879
BssMI GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
BssT1I CCWWGG 2 cut(s) 1120, 1879
Bst2UI CCWGG 3 cut(s) 361, 611, 920
Bst6I CTCTTC 4 cut(s) 150, 812, 1855, 1868
BstAFI CTTAAG 3 cut(s) 935, 1532, 1685
BstAPI GCANNNNNTGC 1 cut(s) 1928
BstBAI YACGTR 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 4 cut(s) 39, 249, 696, 1516
BstDEI CTNAG 7 cut(s) 101, 134, 194, 378, 681, 1613, 1965
BstEII GGTNACC 1 cut(s) 446
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 6 cut(s) 847, 869, 874, 1220, 1429, 1617
BstH2I RGCGCY 1 cut(s) 1627
BstHHI GCGC 4 cut(s) 120, 1133, 1626, 1765
BstKTI GATC 6 cut(s) 259, 366, 672, 934, 1284, 1429
BstMAI GTCTC 3 cut(s) 192, 1488, 1832
BstMBI GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
BstMWI GCNNNNNNNGC 5 cut(s) 11, 523, 1623, 1919, 1928
BstNI CCWGG 3 cut(s) 361, 611, 920
BstNSI RCATGY 1 cut(s) 1769
BstPI GGTNACC 1 cut(s) 446
BstSCI CCNGG 3 cut(s) 359, 609, 918
BstSFI CTRYAG 1 cut(s) 812
BstV1I GCAGC 3 cut(s) 287, 1140, 1306
BstV2I GAAGAC 1 cut(s) 1281
BstX2I RGATCY 1 cut(s) 931
BstYI RGATCY 1 cut(s) 931
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 1 cut(s) 1362
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 6 cut(s) 847, 869, 874, 1220, 1429, 1617
BtsI GCAGTG 1 cut(s) 1920
BtsIMutI CAGTG 4 cut(s) 1272, 1363, 1634, 1920
BveI ACCTGC 2 cut(s) 434, 1404
Cac8I GCNNGC 4 cut(s) 39, 249, 696, 1516
CaiI CAGNNNCTG 2 cut(s) 919, 1300
CciI TCATGA 1 cut(s) 1869
CfoI GCGC 4 cut(s) 120, 1133, 1626, 1765
Cfr10I RCCGGY 1 cut(s) 1514
Cfr13I GGNCC 2 cut(s) 460, 1060
Csp6I GTAC 2 cut(s) 1333, 1463
CviAII CATG 9 cut(s) 121, 254, 260, 772, 1733, 1739, 1766, 1870, 1903
CviQI GTAC 2 cut(s) 1333, 1463
DdeI CTNAG 7 cut(s) 101, 134, 194, 378, 681, 1613, 1965
DpnI GATC 6 cut(s) 258, 365, 671, 933, 1283, 1428
DpnII GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
Eam1104I CTCTTC 4 cut(s) 150, 812, 1855, 1868
EarI CTCTTC 4 cut(s) 150, 812, 1855, 1868
EciI GGCGGA 3 cut(s) 20, 386, 1533
Eco130I CCWWGG 2 cut(s) 1120, 1879
Eco32I GATATC 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 460, 1060
Eco57I CTGAAG 4 cut(s) 321, 896, 1248, 1307
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 945
Eco91I GGTNACC 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 1060
EcoO65I GGTNACC 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 359, 609, 918
EcoRV GATATC 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 1120, 1879
EcoT22I ATGCAT 3 cut(s) 151, 1252, 1574
ErhI CCWWGG 2 cut(s) 1120, 1879
FaeI CATG 9 cut(s) 124, 257, 263, 775, 1736, 1742, 1769, 1873, 1906
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 2 cut(s) 1631, 1754
FatI CATG 9 cut(s) 120, 253, 259, 771, 1732, 1738, 1765, 1869, 1902
FauNDI CATATG 2 cut(s) 151, 1048
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 301, 1129, 1295
FokI GGATG 6 cut(s) 854, 876, 881, 1207, 1436, 1604
Fsp4HI GCNGC 3 cut(s) 301, 1129, 1295
FspBI CTAG 7 cut(s) 485, 695, 1157, 1460, 1823, 1932, 2006
FspI TGCGCA 1 cut(s) 1764
GlaI GCGC 4 cut(s) 119, 1132, 1625, 1764
GluI GCNGC 3 cut(s) 301, 1129, 1295
GsaI CCCAGC 1 cut(s) 1151
GsuI CTGGAG 3 cut(s) 134, 343, 1658
HaeII RGCGCY 1 cut(s) 1627
HapII CCGG 2 cut(s) 1515, 1621
HhaI GCGC 4 cut(s) 120, 1133, 1626, 1765
Hin1II CATG 9 cut(s) 124, 257, 263, 775, 1736, 1742, 1769, 1873, 1906
Hin6I GCGC 4 cut(s) 118, 1131, 1624, 1763
HinP1I GCGC 4 cut(s) 118, 1131, 1624, 1763
HindIII AAGCTT 4 cut(s) 35, 290, 908, 1529
HinfI GANTC 2 cut(s) 393, 1720
HpaII CCGG 2 cut(s) 1515, 1621
HphI GGTGA 3 cut(s) 482, 1126, 1777
Hpy166II GTNNAC 2 cut(s) 560, 1117
Hpy188I TCNGA 6 cut(s) 340, 379, 743, 915, 1558, 1938
Hpy8I GTNNAC 2 cut(s) 560, 1117
HpyAV CCTTC 6 cut(s) 251, 871, 1100, 1383, 1451, 1484
HpyCH4IV ACGT 2 cut(s) 562, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 523, 1623, 1919, 1928
HpyF3I CTNAG 7 cut(s) 101, 134, 194, 378, 681, 1613, 1965
HpySE526I ACGT 2 cut(s) 562, 661
Hsp92II CATG 9 cut(s) 124, 257, 263, 775, 1736, 1742, 1769, 1873, 1906
HspAI GCGC 4 cut(s) 118, 1131, 1624, 1763
KroI GCCGGC 1 cut(s) 1514
KroNI GCCGGC 1 cut(s) 1516
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
LguI GCTCTTC 1 cut(s) 1868
LmnI GCTCC 6 cut(s) 115, 437, 732, 1076, 1410, 1511
Lsp1109I GCAGC 3 cut(s) 287, 1140, 1306
LweI GCATC 8 cut(s) 136, 154, 312, 463, 619, 859, 1559, 1626
MaeI CTAG 7 cut(s) 485, 695, 1157, 1460, 1823, 1932, 2006
MaeII ACGT 2 cut(s) 562, 661
MaeIII GTNAC 4 cut(s) 342, 446, 949, 1783
MalI GATC 6 cut(s) 258, 365, 671, 933, 1283, 1428
MboI GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
MflI RGATCY 1 cut(s) 931
MhlI GDGCHC 1 cut(s) 1964
MluCI AATT 8 cut(s) 90, 233, 330, 404, 656, 798, 1923, 1941
MlyI GAGTC 2 cut(s) 387, 1714
MmeI TCCRAC 2 cut(s) 435, 1006
Mph1103I ATGCAT 3 cut(s) 151, 1252, 1574
MroNI GCCGGC 1 cut(s) 1514
MroXI GAANNNNTTC 5 cut(s) 222, 334, 650, 1035, 1898
MslI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1112, 1577, 1737, 1743
MspA1I CMGCKG 1 cut(s) 787
MspCI CTTAAG 3 cut(s) 935, 1532, 1685
MspI CCGG 2 cut(s) 1515, 1621
MspR9I CCNGG 3 cut(s) 361, 611, 920
Mva1269I GAATGC 2 cut(s) 940, 1436
MvaI CCWGG 3 cut(s) 361, 611, 920
MwoI GCNNNNNNNGC 5 cut(s) 11, 523, 1623, 1919, 1928
NaeI GCCGGC 1 cut(s) 1516
NdeI CATATG 2 cut(s) 151, 1048
NdeII GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
NgoMIV GCCGGC 1 cut(s) 1514
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 9 cut(s) 124, 257, 263, 775, 1736, 1742, 1769, 1873, 1906
NlaIV GGNNCC 1 cut(s) 1513
NmuCI GTSAC 1 cut(s) 1783
NsbI TGCGCA 1 cut(s) 1764
NsiI ATGCAT 3 cut(s) 151, 1252, 1574
NspI RCATGY 1 cut(s) 1769
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 213
PaeR7I CTCGAG 1 cut(s) 945
PagI TCATGA 1 cut(s) 1869
PciSI GCTCTTC 1 cut(s) 1868
PctI GAATGC 2 cut(s) 940, 1436
PdiI GCCGGC 1 cut(s) 1516
PdmI GAANNNNTTC 5 cut(s) 222, 334, 650, 1035, 1898
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 3 cut(s) 302, 1130, 1296
PleI GAGTC 2 cut(s) 387, 1714
PpsI GAGTC 2 cut(s) 387, 1714
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 1060
Psp5II RGGWCCY 1 cut(s) 1060
Psp6I CCWGG 3 cut(s) 359, 609, 918
PspEI GGTNACC 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 1147
PspGI CCWGG 3 cut(s) 359, 609, 918
PspN4I GGNNCC 1 cut(s) 1513
PspPI GGNCC 2 cut(s) 460, 1060
PspPPI RGGWCCY 1 cut(s) 1060
PstNI CAGNNNCTG 2 cut(s) 919, 1300
PsuI RGATCY 1 cut(s) 931
PvuII CAGCTG 1 cut(s) 787
RsaI GTAC 2 cut(s) 1334, 1464
RsaNI GTAC 2 cut(s) 1333, 1463
RseI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1112, 1577, 1737, 1743
SapI GCTCTTC 1 cut(s) 1868
SatI GCNGC 3 cut(s) 301, 1129, 1295
Sau3AI GATC 6 cut(s) 256, 363, 669, 931, 1281, 1426
Sau96I GGNCC 2 cut(s) 460, 1060
SchI GAGTC 2 cut(s) 387, 1714
ScrFI CCNGG 3 cut(s) 361, 611, 920
SduI GDGCHC 1 cut(s) 1964
SfaNI GCATC 8 cut(s) 136, 154, 312, 463, 619, 859, 1559, 1626
SfcI CTRYAG 1 cut(s) 812
Sfr274I CTCGAG 1 cut(s) 945
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 2 cut(s) 460, 1060
SlaI CTCGAG 1 cut(s) 945
SmiMI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1112, 1577, 1737, 1743
SmlI CTYRAG 8 cut(s) 427, 935, 945, 1469, 1532, 1685, 1792, 1831
SmoI CTYRAG 8 cut(s) 427, 935, 945, 1469, 1532, 1685, 1792, 1831
SpeI ACTAGT 1 cut(s) 1156
Sse9I AATT 8 cut(s) 90, 233, 330, 404, 656, 798, 1923, 1941
SsiI CCGC 6 cut(s) 5, 14, 167, 397, 698, 1518
SspI AATATT 1 cut(s) 1448
SspMI CTAG 7 cut(s) 485, 695, 1157, 1460, 1823, 1932, 2006
StyD4I CCNGG 3 cut(s) 359, 609, 918
StyI CCWWGG 2 cut(s) 1120, 1879
TaiI ACGT 2 cut(s) 565, 664
TaqI TCGA 7 cut(s) 51, 322, 328, 466, 946, 1548, 1567
TasI AATT 8 cut(s) 90, 233, 330, 404, 656, 798, 1923, 1941
TatI WGTACW 2 cut(s) 1332, 1462
TscAI CASTG 4 cut(s) 1272, 1370, 1641, 1920
TseFI GTSAC 1 cut(s) 1783
TseI GCWGC 3 cut(s) 300, 1128, 1294
Tsp45I GTSAC 1 cut(s) 1783
TspRI CASTG 4 cut(s) 1272, 1370, 1641, 1920
Vha464I CTTAAG 3 cut(s) 935, 1532, 1685
VpaK11BI GGWCC 2 cut(s) 460, 1060
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 3 cut(s) 90, 233, 404
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 1 cut(s) 1769
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XhoI CTCGAG 1 cut(s) 945
XmnI GAANNNNTTC 5 cut(s) 222, 334, 650, 1035, 1898
XspI CTAG 7 cut(s) 485, 695, 1157, 1460, 1823, 1932, 2006
Zsp2I ATGCAT 3 cut(s) 151, 1252, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.