Rmu_sc0005478.1_g000003

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005478.1
OG0000888
Physical Location & Seq
Forward (+)
7829 .. 10678
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005478.1_g000003.1.cds

Sequence Viewer

Length: 2850 bp
atggcggagagtgcagtaaagtttctacttgacaagcttgcacctcttttcgaaaatgagttgcaacttttgaggggggttagggaagaaattttgcatctaagaggagaactggagcgcatgacggctttcctgagggttgctgatgcatatgaagagagtgatgcggaactcaaagtatgggttaaacaactaagagacattgctcacgaaagtgaagatgttcttgatgaatttacccttctgcaagcacatgatcatgatcatggggagggattatatggttcaatccataagctttcttgctgcatcaagaatacaaaagctcgatatcgaattgcttcggagttacgagccataaactccaggatcaaaaaaatctctgaggttcataagcgactccgccacaaattcaatatggatgaacaaggctcaagctccaaaagtgcaggttacctgttggaggaccatcgaggtgatgctctacttctagtgaaaactgatatagtgggcatagacgagcctattaagcagatggttgggaggctggtcaatggtggttgtggacgtgaggtagtttctgtggcaggaatgggaggaatggggaaaactaccctggcgaagcaagtctatgatgcagcagaagtgaagaaacatttcaaattacgtgcttggatcacttttactcagtctttcaggctagcggaacttctcaaagacatcattcaacagctccaccaagcaatccgaagtccagttccacaagaaatcaatagcatgaataccaaccagctgaaaacaataattaaagacttcctacagaagaggaggtatctcattgtcttggatgatgtgtggcacttatatggatgggatgccttgaagtatgcactgcctaataatatttacggcagcagagtgatactaactactcgaaatgcagatatagcctccacaacaagcacggaatctggaggtgaagtctatactttgaagcccttgcccctagtggagtcatgggagctcctgtgcaagaagacattccaagggaattcctgccctcctcatttagagaaaatctgtaactatgttctgagaaagtgtgaggggctaccccttgctattgtggcaattagtggagttctggctgctaaagataagcgcagaattgatgagtgggaaatggttggccacagtctcggggctgaaattgagggcaatgacaaactgcaggacttgaagaaagtactttcactcagtttcaatgatttaccatactatctaaaatcgtgcttcttgtacctaagcatctttccagaggaccatctaattaagcatatgaggcttattcgattatggatggcagagggcttcattgaagaaaaacaaggaaagacattggaagaggttgcagaggattacctcaatgaactattgaataggagcatgattcaagcagcagagacaacagctgatggaagggtcacaaaatttcgtatccatgatctcttccgcgaaattatcacctccaagacgagaagtcagaactttgccacaatagccaaagagcagaatataacattgccagaaaaaatccgtcgcctgtcaatacataacacactgcaatatgttcagacaaacaggtgtgcttctcaactacgttctctttttatgttcacagttgctgagaagccatcactacaaagactattcccagccggctttgcgctgcttaatgtgttagatttgcaaagtacacctttaaatgttttcccagttgaagttgtgaatctttactttttaaagtatctgagcttgagagataccagggtaaaaaccattccaagatttatagggaagcttcagaacctggaaactttggatcttaagcattctcaagttactgaactcccagttgaaattctgaaactccaacatctgcgctatctcttggtatatcgttatgagtttgttccttacgaagattttcactcaacatatggttttaaggtccttgcaaaaataggagctttgaaatcccttcaaaagctatgtttcattgaggtgaaccaaggtggcagcgcattattgagagagctggggaaactagttcagttgagaaggttaggcattgttaagatgaggaaaaaagatggcaaggctctttcttcatccattgaaaagttgtccaaactttatgcattgtccataacttcagtggaagaagacgagatcattgatttgcagcacctgtgttctcctcctctattgcttcagcatttgtacttgcgaggcagactggaagcattacctcactggataccttcactccatagcctcgtcaagttgtatttgaaatggagcaggttaagggatgatccacttgcattccttcagtatttgcccaatctagtacatcttgagttgtgtcaggtttttgaaggagacacactatgttttggagccggcggatttaagaagctcaagcatttatgcctcgataaatctgatgaacttcgatgcatagagatggaggtgggaacaatgccttgtattgaaaatctaatcatccggcgctgtaaatcactggagaaagtcccatcaggcattgaacatctcatcaaactaaagttgctcaagttttttgatatgccagaaaaactaatcaagactctacttccacatgagcatggcaatgattattggaaagttgcgcatgtcccagaagtttatgtcacctacttgagagagtttgggtgggaagtattctcattagagggcttgagtgagacacaaatctcgtctcaacccagttctgtcatgaagagcattggaaccggttggaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

949

Amino Acids

108.8

Weight (kDa)

8.72

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2715
Acc36I ACCTGC 2 cut(s) 440, 2355
AccII CGCG 1 cut(s) 1515
AciI CCGC 6 cut(s) 5, 167, 403, 704, 1513, 2469
AclWI GGATC 4 cut(s) 377, 683, 1890, 2372
AcoI YGGCCR 1 cut(s) 1189
AcsI RAATTY 6 cut(s) 90, 233, 410, 1051, 1490, 1920
AcuI CTGAAG 4 cut(s) 1847, 2199, 2258, 2378
AfaI GTAC 5 cut(s) 1248, 1301, 1756, 2285, 2415
AfiI CCNNNNNNNGG 1 cut(s) 463
AflII CTTAAG 1 cut(s) 1886
AgeI ACCGGT 1 cut(s) 2837
AhdI GACNNNNNGTC 1 cut(s) 1539
AhlI ACTAGT 1 cut(s) 2107
AjiI CACGTC 1 cut(s) 569
AjnI CCWGG 4 cut(s) 365, 615, 1826, 1869
AleI CACNNNNGTG 1 cut(s) 213
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 6 cut(s) 192, 1202, 1457, 2439, 2783, 2808
AlwI GGATC 4 cut(s) 377, 683, 1890, 2372
AlwNI CAGNNNCTG 3 cut(s) 1685, 1870, 2251
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 1 cut(s) 1189
ApeKI GCWGC 8 cut(s) 306, 638, 912, 1148, 1457, 1729, 2079, 2245
ApoI RAATTY 6 cut(s) 90, 233, 410, 1051, 1490, 1920
AsiGI ACCGGT 1 cut(s) 2837
Asp700I GAANNNNTTC 5 cut(s) 222, 340, 656, 1040, 1986
AspLEI GCGC 7 cut(s) 120, 1164, 1729, 1944, 2084, 2577, 2716
AspS9I GGNCC 3 cut(s) 466, 1321, 2011
AsuHPI GGTGA 5 cut(s) 488, 989, 1516, 2077, 2728
AsuII TTCGAA 1 cut(s) 51
AsuNHI GCTAGC 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 3 cut(s) 466, 1321, 2011
AxyI CCTNAGG 1 cut(s) 134
BaeI ACNNNNGTAYC 2 cut(s) 1815, 1848
BalI TGGCCA 1 cut(s) 1191
BanII GRGCYC 1 cut(s) 1026
BbsI GAAGAC 2 cut(s) 1043, 2232
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 8 cut(s) 293, 650, 924, 1135, 1469, 1716, 2091, 2257
BceAI ACGGC 2 cut(s) 141, 925
BciT130I CCWGG 4 cut(s) 367, 617, 1828, 1871
BciVI GTATCC 2 cut(s) 1508, 2313
BclI TGATCA 2 cut(s) 256, 262
BcoDI GTCTC 6 cut(s) 192, 1202, 1457, 2439, 2783, 2808
BcuI ACTAGT 1 cut(s) 2107
BfaI CTAG 5 cut(s) 491, 701, 1007, 2108, 2411
BfmI CTRYAG 2 cut(s) 818, 1229
BfoI RGCGCY 1 cut(s) 2578
BfrI CTTAAG 1 cut(s) 1886
BfuAI ACCTGC 2 cut(s) 440, 2355
BfuI GTATCC 2 cut(s) 1508, 2313
BisI GCNGC 8 cut(s) 307, 639, 913, 1149, 1458, 1730, 2080, 2246
BlsI GCNGC 8 cut(s) 308, 640, 914, 1150, 1459, 1731, 2081, 2247
BmcAI AGTACT 1 cut(s) 1248
Bme1390I CCNGG 4 cut(s) 367, 617, 1828, 1871
Bme18I GGWCC 3 cut(s) 466, 1321, 2011
BmeRI GACNNNNNGTC 1 cut(s) 1539
BmeT110I CYCGRG 1 cut(s) 1199
BmgBI CACGTC 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 466, 1321, 2011
BmiI GGNNCC 2 cut(s) 2464, 2836
BmrFI CCNGG 4 cut(s) 367, 617, 1828, 1871
BmrI ACTGGG 3 cut(s) 1769, 1907, 2805
BmsI GCATC 9 cut(s) 106, 136, 154, 318, 469, 625, 865, 1317, 2510
BmtI GCTAGC 1 cut(s) 704
BmuI ACTGGG 3 cut(s) 1769, 1907, 2805
BpiI GAAGAC 2 cut(s) 1043, 2232
BpmI CTGGAG 4 cut(s) 134, 349, 993, 2609
Bpu10I CCTNAGC 1 cut(s) 1304
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 8 cut(s) 418, 1837, 1881, 2441, 2468, 2621, 2764, 2803
BsaAI YACGTR 1 cut(s) 668
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 615, 1045, 1827, 2071
BsaWI WCCGGW 1 cut(s) 2837
BsaXI ACNNNNNCTCC 6 cut(s) 966, 996, 1013, 1043, 2313, 2343
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse118I RCCGGY 3 cut(s) 1718, 2465, 2837
Bse1I ACTGG 8 cut(s) 117, 755, 1775, 1913, 2304, 2321, 2592, 2811
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 4 cut(s) 201, 1225, 1580, 2701
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 4 cut(s) 367, 617, 1828, 1871
BseDI CCNNGG 4 cut(s) 615, 1045, 1827, 2071
BseGI GGATG 8 cut(s) 427, 853, 875, 880, 1365, 2171, 2380, 2568
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 463
BseMI GCAATG 4 cut(s) 201, 1225, 1580, 2701
BseMII CTCAG 7 cut(s) 125, 375, 701, 1085, 1270, 1677, 1802
BseNI ACTGG 8 cut(s) 117, 755, 1775, 1913, 2304, 2321, 2592, 2811
BseRI GAGGAG 5 cut(s) 120, 841, 1053, 2250, 2253
BseXI GCAGC 8 cut(s) 293, 650, 924, 1135, 1469, 1716, 2091, 2257
BseYI CCCAGC 2 cut(s) 1714, 2098
BsgI GTGCAG 2 cut(s) 33, 468
Bsh1236I CGCG 1 cut(s) 1515
BshFI GGCC 1 cut(s) 1191
BshTI ACCGGT 1 cut(s) 2837
BsiHKAI GWGCWC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 1199
BsiSI CCGG 4 cut(s) 1719, 2466, 2572, 2838
BslFI GGGAC 2 cut(s) 2582, 2705
BslI CCNNNNNNNGG 1 cut(s) 463
BsmAI GTCTC 6 cut(s) 192, 1202, 1457, 2439, 2783, 2808
BsmBI CGTCTC 1 cut(s) 2808
BsmFI GGGAC 2 cut(s) 2582, 2705
BsmI GAATGC 2 cut(s) 1891, 2387
BsnI GGCC 1 cut(s) 1191
BsoBI CYCGRG 1 cut(s) 1199
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp143I GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
BspACI CCGC 6 cut(s) 5, 167, 403, 704, 1513, 2469
BspANI GGCC 1 cut(s) 1191
BspCNI CTCAG 7 cut(s) 126, 376, 700, 1086, 1269, 1678, 1803
BspFNI CGCG 1 cut(s) 1515
BspHI TCATGA 2 cut(s) 259, 2820
BspLI GGNNCC 2 cut(s) 2464, 2836
BspMAI CTGCAG 1 cut(s) 1233
BspMI ACCTGC 2 cut(s) 440, 2355
BspOI GCTAGC 1 cut(s) 704
BspPI GGATC 4 cut(s) 377, 683, 1890, 2372
BspQI GCTCTTC 1 cut(s) 2819
BspT104I TTCGAA 1 cut(s) 51
BspTI CTTAAG 1 cut(s) 1886
BsrDI GCAATG 4 cut(s) 201, 1225, 1580, 2701
BsrFI RCCGGY 3 cut(s) 1718, 2465, 2837
BsrI ACTGG 8 cut(s) 117, 755, 1775, 1913, 2304, 2321, 2592, 2811
BssAI RCCGGY 3 cut(s) 1718, 2465, 2837
BssECI CCNNGG 4 cut(s) 615, 1045, 1827, 2071
BssMI GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
BssT1I CCWWGG 2 cut(s) 1045, 2071
Bst2UI CCWGG 4 cut(s) 367, 617, 1828, 1871
Bst4CI ACNGT 2 cut(s) 1196, 1681
Bst6I CTCTTC 5 cut(s) 150, 818, 1398, 1514, 2819
BstAFI CTTAAG 1 cut(s) 1886
BstBAI YACGTR 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 5 cut(s) 39, 249, 702, 1720, 2467
BstEII GGTNACC 1 cut(s) 452
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 8 cut(s) 427, 853, 875, 880, 1365, 2171, 2380, 2568
BstFNI CGCG 1 cut(s) 1515
BstH2I RGCGCY 1 cut(s) 2578
BstHHI GCGC 7 cut(s) 120, 1164, 1729, 1944, 2084, 2577, 2716
BstKTI GATC 8 cut(s) 259, 265, 372, 678, 1507, 1885, 2235, 2380
BstMAI GTCTC 6 cut(s) 192, 1202, 1457, 2439, 2783, 2808
BstMBI GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
BstMWI GCNNNNNNNGC 7 cut(s) 11, 529, 947, 1127, 1342, 1559, 1724
BstNI CCWGG 4 cut(s) 367, 617, 1828, 1871
BstNSI RCATGY 1 cut(s) 2720
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 4 cut(s) 365, 615, 1826, 1869
BstSFI CTRYAG 2 cut(s) 818, 1229
BstUI CGCG 1 cut(s) 1515
BstV1I GCAGC 8 cut(s) 293, 650, 924, 1135, 1469, 1716, 2091, 2257
BstV2I GAAGAC 2 cut(s) 1043, 2232
BstX2I RGATCY 1 cut(s) 1882
BstYI RGATCY 1 cut(s) 1882
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 2 cut(s) 1508, 2313
BsuRI GGCC 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 569
BtsCI GGATG 8 cut(s) 427, 853, 875, 880, 1365, 2171, 2380, 2568
BtsI GCAGTG 2 cut(s) 890, 1619
BtsIMutI CAGTG 5 cut(s) 890, 1619, 2223, 2314, 2585
BveI ACCTGC 2 cut(s) 440, 2355
Cac8I GCNNGC 5 cut(s) 39, 249, 702, 1720, 2467
CaiI CAGNNNCTG 3 cut(s) 1685, 1870, 2251
CciI TCATGA 2 cut(s) 259, 2820
CfoI GCGC 7 cut(s) 120, 1164, 1729, 1944, 2084, 2577, 2716
Cfr10I RCCGGY 3 cut(s) 1718, 2465, 2837
Cfr13I GGNCC 3 cut(s) 466, 1321, 2011
Csp6I GTAC 5 cut(s) 1247, 1300, 1755, 2284, 2414
CspAI ACCGGT 1 cut(s) 2837
CviQI GTAC 5 cut(s) 1247, 1300, 1755, 2284, 2414
DpnI GATC 8 cut(s) 258, 264, 371, 677, 1506, 1884, 2234, 2379
DpnII GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
DraI TTTAAA 2 cut(s) 1764, 1803
DriI GACNNNNNGTC 1 cut(s) 1539
EaeI YGGCCR 1 cut(s) 1189
Eam1104I CTCTTC 5 cut(s) 150, 818, 1398, 1514, 2819
Eam1105I GACNNNNNGTC 1 cut(s) 1539
EarI CTCTTC 5 cut(s) 150, 818, 1398, 1514, 2819
EciI GGCGGA 3 cut(s) 20, 392, 2484
Ecl136II GAGCTC 1 cut(s) 1024
Eco130I CCWWGG 2 cut(s) 1045, 2071
Eco24I GRGCYC 1 cut(s) 1026
Eco32I GATATC 1 cut(s) 332
Eco47I GGWCC 3 cut(s) 466, 1321, 2011
Eco53kI GAGCTC 1 cut(s) 1024
Eco57I CTGAAG 4 cut(s) 1847, 2199, 2258, 2378
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 1199
Eco91I GGTNACC 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 1024
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 2011
EcoO65I GGTNACC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 1051
EcoRII CCWGG 4 cut(s) 365, 615, 1826, 1869
EcoRV GATATC 1 cut(s) 332
EcoT14I CCWWGG 2 cut(s) 1045, 2071
EcoT22I ATGCAT 3 cut(s) 151, 2203, 2525
EcoT38I GRGCYC 1 cut(s) 1026
ErhI CCWWGG 2 cut(s) 1045, 2071
Esp3I CGTCTC 1 cut(s) 2808
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 1744, 1776
FaqI GGGAC 2 cut(s) 2582, 2705
FauNDI CATATG 3 cut(s) 151, 1338, 1999
FbaI TGATCA 2 cut(s) 256, 262
Fnu4HI GCNGC 8 cut(s) 307, 639, 913, 1149, 1458, 1730, 2080, 2246
FokI GGATG 8 cut(s) 434, 860, 882, 887, 1372, 2158, 2387, 2555
FriOI GRGCYC 1 cut(s) 1026
Fsp4HI GCNGC 8 cut(s) 307, 639, 913, 1149, 1458, 1730, 2080, 2246
FspBI CTAG 5 cut(s) 491, 701, 1007, 2108, 2411
FspI TGCGCA 1 cut(s) 2715
GlaI GCGC 7 cut(s) 119, 1163, 1728, 1943, 2083, 2576, 2715
GluI GCNGC 8 cut(s) 307, 639, 913, 1149, 1458, 1730, 2080, 2246
GsaI CCCAGC 2 cut(s) 1718, 2102
GsuI CTGGAG 4 cut(s) 134, 349, 993, 2609
HaeII RGCGCY 1 cut(s) 2578
HaeIII GGCC 1 cut(s) 1191
HapII CCGG 4 cut(s) 1719, 2466, 2572, 2838
HhaI GCGC 7 cut(s) 120, 1164, 1729, 1944, 2084, 2577, 2716
Hin6I GCGC 7 cut(s) 118, 1162, 1727, 1942, 2082, 2575, 2714
HinP1I GCGC 7 cut(s) 118, 1162, 1727, 1942, 2082, 2575, 2714
HindIII AAGCTT 3 cut(s) 35, 296, 1859
HinfI GANTC 6 cut(s) 399, 968, 1013, 1450, 1789, 2671
HpaII CCGG 4 cut(s) 1719, 2466, 2572, 2838
HphI GGTGA 5 cut(s) 488, 989, 1516, 2077, 2728
Hpy166II GTNNAC 4 cut(s) 566, 1677, 1757, 2068
Hpy8I GTNNAC 4 cut(s) 566, 1677, 1757, 2068
Hpy99I CGWCG 1 cut(s) 1602
HpyAV CCTTC 7 cut(s) 251, 1473, 2051, 2115, 2334, 2402, 2435
HpyCH4III ACNGT 2 cut(s) 1196, 1681
HpyCH4IV ACGT 3 cut(s) 568, 667, 1660
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 529, 947, 1127, 1342, 1559, 1724
HpySE526I ACGT 3 cut(s) 568, 667, 1660
HspAI GCGC 7 cut(s) 118, 1162, 1727, 1942, 2082, 2575, 2714
KroI GCCGGC 2 cut(s) 1718, 2465
KroNI GCCGGC 2 cut(s) 1720, 2467
Ksp22I TGATCA 2 cut(s) 256, 262
Kzo9I GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
LguI GCTCTTC 1 cut(s) 2819
LmnI GCTCC 9 cut(s) 115, 443, 738, 1021, 1029, 1443, 2027, 2361, 2462
Lsp1109I GCAGC 8 cut(s) 293, 650, 924, 1135, 1469, 1716, 2091, 2257
LweI GCATC 9 cut(s) 106, 136, 154, 318, 469, 625, 865, 1317, 2510
MaeI CTAG 5 cut(s) 491, 701, 1007, 2108, 2411
MaeII ACGT 3 cut(s) 568, 667, 1660
MaeIII GTNAC 6 cut(s) 348, 452, 1082, 1483, 1900, 2734
MalI GATC 8 cut(s) 258, 264, 371, 677, 1506, 1884, 2234, 2379
MboI GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
MflI RGATCY 1 cut(s) 1882
MhlI GDGCHC 1 cut(s) 1026
MlsI TGGCCA 1 cut(s) 1191
MluNI TGGCCA 1 cut(s) 1191
MlyI GAGTC 3 cut(s) 393, 1022, 2665
MmeI TCCRAC 3 cut(s) 441, 1957, 2822
Mox20I TGGCCA 1 cut(s) 1191
Mph1103I ATGCAT 3 cut(s) 151, 2203, 2525
MroNI GCCGGC 2 cut(s) 1718, 2465
MroXI GAANNNNTTC 5 cut(s) 222, 340, 656, 1040, 1986
MscI TGGCCA 1 cut(s) 1191
MslI CAYNNNNRTG 9 cut(s) 213, 258, 264, 474, 864, 2063, 2528, 2688, 2694
Msp20I TGGCCA 1 cut(s) 1191
MspA1I CMGCKG 2 cut(s) 793, 1472
MspCI CTTAAG 1 cut(s) 1886
MspI CCGG 4 cut(s) 1719, 2466, 2572, 2838
MspR9I CCNGG 4 cut(s) 367, 617, 1828, 1871
Mva1269I GAATGC 2 cut(s) 1891, 2387
MvaI CCWGG 4 cut(s) 367, 617, 1828, 1871
MvnI CGCG 1 cut(s) 1515
MwoI GCNNNNNNNGC 7 cut(s) 11, 529, 947, 1127, 1342, 1559, 1724
NaeI GCCGGC 2 cut(s) 1720, 2467
NdeI CATATG 3 cut(s) 151, 1338, 1999
NdeII GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
NgoMIV GCCGGC 2 cut(s) 1718, 2465
NheI GCTAGC 1 cut(s) 700
NlaIV GGNNCC 2 cut(s) 2464, 2836
NmuCI GTSAC 2 cut(s) 1483, 2734
NsbI TGCGCA 1 cut(s) 2715
NsiI ATGCAT 3 cut(s) 151, 2203, 2525
NspI RCATGY 1 cut(s) 2720
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 213
PagI TCATGA 2 cut(s) 259, 2820
PciSI GCTCTTC 1 cut(s) 2819
PctI GAATGC 2 cut(s) 1891, 2387
PdiI GCCGGC 2 cut(s) 1720, 2467
PdmI GAANNNNTTC 5 cut(s) 222, 340, 656, 1040, 1986
PfeI GAWTC 3 cut(s) 968, 1450, 1789
PfoI TCCNGGA 1 cut(s) 365
PinAI ACCGGT 1 cut(s) 2837
PkrI GCNGC 8 cut(s) 308, 640, 914, 1150, 1459, 1731, 2081, 2247
PleI GAGTC 3 cut(s) 393, 1021, 2665
PpsI GAGTC 3 cut(s) 393, 1021, 2665
Ppu21I YACGTR 1 cut(s) 668
PpuMI RGGWCCY 1 cut(s) 2011
Psp124BI GAGCTC 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 2011
Psp6I CCWGG 4 cut(s) 365, 615, 1826, 1869
PspEI GGTNACC 1 cut(s) 452
PspFI CCCAGC 2 cut(s) 1714, 2098
PspGI CCWGG 4 cut(s) 365, 615, 1826, 1869
PspN4I GGNNCC 2 cut(s) 2464, 2836
PspPI GGNCC 3 cut(s) 466, 1321, 2011
PspPPI RGGWCCY 1 cut(s) 2011
PstI CTGCAG 1 cut(s) 1233
PstNI CAGNNNCTG 3 cut(s) 1685, 1870, 2251
PsuI RGATCY 1 cut(s) 1882
PvuII CAGCTG 2 cut(s) 793, 1472
RsaI GTAC 5 cut(s) 1248, 1301, 1756, 2285, 2415
RsaNI GTAC 5 cut(s) 1247, 1300, 1755, 2284, 2414
RseI CAYNNNNRTG 9 cut(s) 213, 258, 264, 474, 864, 2063, 2528, 2688, 2694
SacI GAGCTC 1 cut(s) 1026
SapI GCTCTTC 1 cut(s) 2819
SatI GCNGC 8 cut(s) 307, 639, 913, 1149, 1458, 1730, 2080, 2246
Sau3AI GATC 8 cut(s) 256, 262, 369, 675, 1504, 1882, 2232, 2377
Sau96I GGNCC 3 cut(s) 466, 1321, 2011
ScaI AGTACT 1 cut(s) 1248
SchI GAGTC 3 cut(s) 393, 1022, 2665
ScrFI CCNGG 4 cut(s) 367, 617, 1828, 1871
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 9 cut(s) 106, 136, 154, 318, 469, 625, 865, 1317, 2510
SfcI CTRYAG 2 cut(s) 818, 1229
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 3 cut(s) 466, 1321, 2011
SmiMI CAYNNNNRTG 9 cut(s) 213, 258, 264, 474, 864, 2063, 2528, 2688, 2694
SmlI CTYRAG 9 cut(s) 433, 1816, 1886, 1896, 2420, 2483, 2636, 2743, 2782
SmoI CTYRAG 9 cut(s) 433, 1816, 1886, 1896, 2420, 2483, 2636, 2743, 2782
SpeI ACTAGT 1 cut(s) 2107
SsiI CCGC 6 cut(s) 5, 167, 403, 704, 1513, 2469
SspI AATATT 1 cut(s) 904
SspMI CTAG 5 cut(s) 491, 701, 1007, 2108, 2411
SstI GAGCTC 1 cut(s) 1026
StyD4I CCNGG 4 cut(s) 365, 615, 1826, 1869
StyI CCWWGG 2 cut(s) 1045, 2071
TaaI ACNGT 2 cut(s) 1196, 1681
TaiI ACGT 3 cut(s) 571, 670, 1663
TaqI TCGA 8 cut(s) 51, 328, 334, 472, 934, 1351, 2499, 2518
TatI WGTACW 4 cut(s) 1246, 1754, 2283, 2413
TfiI GAWTC 3 cut(s) 968, 1450, 1789
TscAI CASTG 5 cut(s) 897, 1626, 2223, 2321, 2592
TseFI GTSAC 2 cut(s) 1483, 2734
TseI GCWGC 8 cut(s) 306, 638, 912, 1148, 1457, 1729, 2079, 2245
Tsp45I GTSAC 2 cut(s) 1483, 2734
TspGWI ACGGA 2 cut(s) 980, 1586
TspRI CASTG 5 cut(s) 897, 1626, 2223, 2321, 2592
Vha464I CTTAAG 1 cut(s) 1886
VpaK11BI GGWCC 3 cut(s) 466, 1321, 2011
XagI CCTNNNNNAGG 1 cut(s) 461
XapI RAATTY 6 cut(s) 90, 233, 410, 1051, 1490, 1920
XceI RCATGY 1 cut(s) 2720
XcmI CCANNNNNNNNNTGG 1 cut(s) 2215
XmnI GAANNNNTTC 5 cut(s) 222, 340, 656, 1040, 1986
XspI CTAG 5 cut(s) 491, 701, 1007, 2108, 2411
ZrmI AGTACT 1 cut(s) 1248
Zsp2I ATGCAT 3 cut(s) 151, 2203, 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.