Rroxscaffold_3G00246790

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000888
Physical Location & Seq
Forward (+)
40055281 .. 40057424
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246790.1

Sequence Viewer

Length: 876 bp
ATGTTGAGGAATTGGACACGGAGTACTTTGTTTTTGATGGAGAGGGACGGATCCAAACCTAATGGAGGGTTCAGACTTCTTAATGTCTTAGACTTGCAAAGTGCGCCTCTAAATGTTTTCCCAGTTAAAGTTATCAACCTTTTCTTTTTGAAGTATCTGAGTTTGAAAGGTACCAAGGTGAAAACCATTCCTAGTTTCATTGGAAACCTTCAAAACCTGGAGACCTTGGATCTTGAACATTCTCTGGTTACTGAACTCCCAGTTGAAATCTTGAAGCTTGAACATCTATGTCATCGCCTGGTATATCGTTACGAATTTGAACATTATAGAGGTTTTCACTCAAAATATGGTTTCAAGGTACTTGCAAAAATTGGAATTTTGATGTCCCTTCAAAAGCTCTGTTTTATCAAGGCAAATGAAGATGATGGTGCCATATTAAGAGAGCTCAGGAACTTAGTTCAGTTAAGAAGGTTAGGAATTGTACAGATGAGGAAAGAAGATGGAAAAATTATTTGTTCATCGATTGAAAAGTTGAACAAACTTTGCGCGTTGTCCATAACTTCAGTAGTAGAAGGTGAGATCATTGATTTGCAGCATCTTTCTTCTCCCCCTCTACTGCTTCAGCGCCTTTACTTGCAAGGACGGTTGGAGACAATACCTCATTGGATTCCTTCTCTGCATAGCCTTGTCAAATTATATTTGAAACAGAGTAGGTTAAAGGATGATCCACTTGTATTCCTTCAATATTTGCCCAATCTAGTACACCTTGAACTATCTCAGGTGTTTGAAGGTGACACGTTATGCTTTGAAGCTGGAGGGTTCGAGAAGCTCAAACATTTGGGTATTTACAAATTTGAGGGACTTGGATGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

291

Amino Acids

33.48

Weight (kDa)

9.02

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 19 - 283 3.2e-39 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 170
AccB1I GGYRCC 2 cut(s) 170, 428
AccII CGCG 1 cut(s) 548
AclWI GGATC 4 cut(s) 45, 58, 237, 719
AcsI RAATTY 3 cut(s) 314, 375, 851
AcuI CTGAAG 2 cut(s) 546, 605
AfaI GTAC 5 cut(s) 25, 172, 360, 483, 762
AfiI CCNNNNNNNGG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 795
AjnI CCWGG 2 cut(s) 216, 297
AluBI AGCT 5 cut(s) 277, 397, 445, 812, 829
AluI AGCT 5 cut(s) 277, 397, 445, 812, 829
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 215, 644
AlwI GGATC 4 cut(s) 45, 58, 237, 719
ApeKI GCWGC 1 cut(s) 592
ApoI RAATTY 3 cut(s) 314, 375, 851
Asp718I GGTACC 1 cut(s) 170
AspLEI GCGC 3 cut(s) 106, 548, 627
AsuHPI GGTGA 3 cut(s) 190, 587, 803
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BamHI GGATCC 1 cut(s) 50
BanI GGYRCC 2 cut(s) 170, 428
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 604
BccI CCATC 3 cut(s) 31, 419, 494
BciT130I CCWGG 2 cut(s) 218, 299
BcoDI GTCTC 2 cut(s) 215, 644
BfaI CTAG 2 cut(s) 192, 758
BfoI RGCGCY 1 cut(s) 628
BisI GCNGC 1 cut(s) 593
BlsI GCNGC 1 cut(s) 594
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 218, 299
BmiI GGNNCC 3 cut(s) 52, 172, 430
BmrFI CCNGG 2 cut(s) 218, 299
BmrI ACTGGG 2 cut(s) 116, 254
BmsI GCATC 2 cut(s) 604, 857
BmuI ACTGGG 2 cut(s) 116, 254
BpmI CTGGAG 2 cut(s) 239, 834
Bpu10I CCTNAGC 1 cut(s) 446
Bsa29I ATCGAT 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 174, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 122, 260
BseBI CCWGG 2 cut(s) 218, 299
BseCI ATCGAT 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 174, 225
BseGI GGATG 2 cut(s) 727, 872
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMII CTCAG 3 cut(s) 149, 460, 791
BseNI ACTGG 2 cut(s) 122, 260
BseXI GCAGC 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 548
BshNI GGYRCC 2 cut(s) 170, 428
BshVI ATCGAT 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 447
BslFI GGGAC 2 cut(s) 59, 370
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 2 cut(s) 215, 644
BsmFI GGGAC 2 cut(s) 59, 370
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 4 cut(s) 50, 229, 579, 724
BspCNI CTCAG 3 cut(s) 150, 459, 790
BspDI ATCGAT 1 cut(s) 521
BspFNI CGCG 1 cut(s) 548
BspLI GGNNCC 3 cut(s) 52, 172, 430
BspPI GGATC 4 cut(s) 45, 58, 237, 719
BspT107I GGYRCC 2 cut(s) 170, 428
BspTNI GGTCTC 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 2 cut(s) 122, 260
BssECI CCNNGG 2 cut(s) 174, 225
BssMI GATC 4 cut(s) 50, 229, 579, 724
BssT1I CCWWGG 2 cut(s) 174, 225
Bst2UI CCWGG 2 cut(s) 218, 299
Bst4CI ACNGT 1 cut(s) 645
BstAUI TGTACA 1 cut(s) 481
BstDEI CTNAG 5 cut(s) 88, 158, 446, 454, 777
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 727, 872
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 3 cut(s) 106, 548, 627
BstKTI GATC 4 cut(s) 53, 232, 582, 727
BstMAI GTCTC 2 cut(s) 215, 644
BstMBI GATC 4 cut(s) 50, 229, 579, 724
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNI CCWGG 2 cut(s) 218, 299
BstSCI CCNGG 2 cut(s) 216, 297
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 1 cut(s) 604
BstX2I RGATCY 2 cut(s) 50, 229
BstYI RGATCY 2 cut(s) 50, 229
Bsu15I ATCGAT 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 521
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 2 cut(s) 727, 872
CfoI GCGC 3 cut(s) 106, 548, 627
ClaI ATCGAT 1 cut(s) 521
Csp6I GTAC 5 cut(s) 24, 171, 359, 482, 761
CviJI RGCY 6 cut(s) 277, 397, 445, 684, 812, 829
CviKI_1 RGCY 6 cut(s) 277, 397, 445, 684, 812, 829
CviQI GTAC 5 cut(s) 24, 171, 359, 482, 761
DdeI CTNAG 5 cut(s) 88, 158, 446, 454, 777
DpnI GATC 4 cut(s) 52, 231, 581, 726
DpnII GATC 4 cut(s) 50, 229, 579, 724
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 174, 225
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 546, 605
EcoICRI GAGCTC 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 216, 297
EcoT14I CCWWGG 2 cut(s) 174, 225
EcoT22I ATGCAT 1 cut(s) 872
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 174, 225
FaqI GGGAC 2 cut(s) 59, 370
Fnu4HI GCNGC 1 cut(s) 593
FokI GGATG 1 cut(s) 734
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 593
FspBI CTAG 2 cut(s) 192, 758
GlaI GCGC 3 cut(s) 105, 547, 626
GluI GCNGC 1 cut(s) 593
GsuI CTGGAG 2 cut(s) 239, 834
HaeII RGCGCY 1 cut(s) 628
HhaI GCGC 3 cut(s) 106, 548, 627
Hin6I GCGC 3 cut(s) 104, 546, 625
HinP1I GCGC 3 cut(s) 104, 546, 625
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 667
HphI GGTGA 3 cut(s) 190, 587, 803
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 2 cut(s) 74, 159
Hpy188III TCNNGA 4 cut(s) 233, 271, 448, 823
Hpy8I GTNNAC 1 cut(s) 763
HpyAV CCTTC 7 cut(s) 218, 398, 462, 566, 681, 749, 782
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 1 cut(s) 797
HpyCH4V TGCA 6 cut(s) 97, 365, 592, 637, 679, 870
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
HpyF3I CTNAG 5 cut(s) 88, 158, 446, 454, 777
HpySE526I ACGT 1 cut(s) 797
HspAI GCGC 3 cut(s) 104, 546, 625
KpnI GGTACC 1 cut(s) 174
Kzo9I GATC 4 cut(s) 50, 229, 579, 724
Lsp1109I GCAGC 1 cut(s) 604
LweI GCATC 2 cut(s) 604, 857
MaeI CTAG 2 cut(s) 192, 758
MaeII ACGT 1 cut(s) 797
MaeIII GTNAC 3 cut(s) 247, 308, 791
MalI GATC 4 cut(s) 52, 231, 581, 726
MboI GATC 4 cut(s) 50, 229, 579, 724
MboII GAAGA 3 cut(s) 431, 509, 594
MflI RGATCY 2 cut(s) 50, 229
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 8 cut(s) 10, 314, 369, 375, 477, 507, 692, 851
MmeI TCCRAC 1 cut(s) 627
MnlI CCTC 9 cut(s) 36, 59, 117, 323, 483, 621, 669, 809, 850
Mph1103I ATGCAT 1 cut(s) 872
MseI TTAA 5 cut(s) 81, 126, 437, 464, 716
MspR9I CCNGG 2 cut(s) 218, 299
MvaI CCWGG 2 cut(s) 218, 299
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 1 cut(s) 103
NdeII GATC 4 cut(s) 50, 229, 579, 724
NlaIV GGNNCC 3 cut(s) 52, 172, 430
NmuCI GTSAC 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 872
PfeI GAWTC 1 cut(s) 667
PkrI GCNGC 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 216, 297
PspGI CCWGG 2 cut(s) 216, 297
PspN4I GGNNCC 3 cut(s) 52, 172, 430
PsuI RGATCY 2 cut(s) 50, 229
RsaI GTAC 5 cut(s) 25, 172, 360, 483, 762
RsaNI GTAC 5 cut(s) 24, 171, 359, 482, 761
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 5 cut(s) 81, 126, 437, 464, 716
SatI GCNGC 1 cut(s) 593
Sau3AI GATC 4 cut(s) 50, 229, 579, 724
ScaI AGTACT 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 218, 299
SduI GDGCHC 1 cut(s) 447
SfaNI GCATC 2 cut(s) 604, 857
Sse9I AATT 8 cut(s) 10, 314, 369, 375, 477, 507, 692, 851
SspI AATATT 1 cut(s) 746
SspMI CTAG 2 cut(s) 192, 758
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 216, 297
StyI CCWWGG 2 cut(s) 174, 225
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 1 cut(s) 800
TaqI TCGA 2 cut(s) 521, 822
TasI AATT 8 cut(s) 10, 314, 369, 375, 477, 507, 692, 851
TatI WGTACW 3 cut(s) 23, 481, 760
TfiI GAWTC 1 cut(s) 667
Tru1I TTAA 5 cut(s) 81, 126, 437, 464, 716
Tru9I TTAA 5 cut(s) 81, 126, 437, 464, 716
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 1 cut(s) 592
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 3 cut(s) 187, 432, 507
TspGWI ACGGA 2 cut(s) 34, 63
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 3 cut(s) 314, 375, 851
XspI CTAG 2 cut(s) 192, 758
ZrmI AGTACT 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.