Rh7BG251100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000888
Physical Location & Seq
Forward (+)
22792303 .. 22795146
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG251100.1

Sequence Viewer

Length: 2139 bp
ATGGCGGAGAGTGCAGTAAAGTTTCTGCTTGACAAGCTTGCACCTCTTTTCGAAAATGAGTTGCAACTTTTGAGAGGGGTTAGGGAAGAAATTTTGTATCTAAGAGGAGAACTGGAGCGCATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAACTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGAGTTACGAGCCATAAACTCCAGGATCAAAAAAATCTCTGAGGTTCATAAGCGACTCCGCCACAAATTCAATATGGCTGAACAAGGCTCAAGCTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAGGTGATGCTTTACTTCTAGAGAAAACTGATATAGTGGGCATAGACGAGCCTATCAAGCAGATGGTTGGGAGGCTGGTTGATGGTGGCTGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGGGAAAACTACCCTGGCGAAGCAAGTCTACGATGCAGCAGAAGTGAAGAAACATTTCAAATTACGTGCTTGGATCACTTTTACTCAGTCTTTCAAGCTAGCGGAACTTCTCAAAGACATCGTTCAACAGCTCCACCAAGCAATCCGAAGTCCAGTTCCACAAGAAATCAATAGCATGAGTACCAACCAGCTGAAAACAATAATTAAAGACTTCCTACAGAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGTATGCACTGCCTAATAATATTTACGGCAGCAGAGTGATACTAACTACTCGAAATGCAGATATAGCCTCCACAACAAGCACAGAATCTGGAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGCGCTGCTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCAGTTGAAGTTGTGAATCTTTACTTTTTAAAGTATCTGAGCTTGAGAGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCGAGTTACTGAACTCCCAGTTGAAATTCTGAAACTCCAACATTTGCGCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTTTCACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGCAGCACCTGTGTTCTCCTCCTCTATTGCTTCAGCATTTGTACTTGCGAGGCAGATTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTCGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTGCCCAATCTAGTACATCTTGAGTTGTGTCAGGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAAGAAGCTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCTTGTATTGAAAATCTAATCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGTTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCATTAGAGGGCTTGAGTGAGACACAAATCTCTTCTCAACCCAGCTCTGTCATGAAGAGCATTGGCACTGGTTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

712

Amino Acids

81.38

Weight (kDa)

8.85

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 1.3e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 328 1.9e-37 NB-ARC domain
AAA_16 PF13191 175 - 283 6.1e-07 AAA ATPase domain
LRR_14 PF23598 337 - 643 3.2e-45 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2004
Acc36I ACCTGC 2 cut(s) 434, 1644
AccI GTMKAC 1 cut(s) 624
AciI CCGC 5 cut(s) 5, 167, 397, 698, 1758
AclWI GGATC 4 cut(s) 371, 677, 1179, 1661
AcsI RAATTY 4 cut(s) 90, 233, 404, 1209
AcuI CTGAAG 4 cut(s) 1136, 1488, 1547, 1667
AfaI GTAC 4 cut(s) 778, 1045, 1574, 1704
AfiI CCNNNNNNNGG 1 cut(s) 457
AflII CTTAAG 2 cut(s) 1175, 1925
AhlI ACTAGT 1 cut(s) 1396
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 4 cut(s) 359, 609, 1115, 1158
AleI CACNNNNGTG 1 cut(s) 213
Alw26I GTCTC 3 cut(s) 192, 1728, 2072
AlwI GGATC 4 cut(s) 371, 677, 1179, 1661
AlwNI CAGNNNCTG 3 cut(s) 965, 1159, 1540
Ama87I CYCGRG 1 cut(s) 1185
ApeKI GCWGC 6 cut(s) 300, 632, 906, 1018, 1368, 1534
ApoI RAATTY 4 cut(s) 90, 233, 404, 1209
Asp700I GAANNNNTTC 4 cut(s) 222, 334, 650, 1275
AspLEI GCGC 6 cut(s) 120, 1018, 1233, 1373, 1866, 2005
AspS9I GGNCC 2 cut(s) 460, 1300
AsuHPI GGTGA 3 cut(s) 482, 1366, 2017
AsuII TTCGAA 1 cut(s) 51
AsuNHI GCTAGC 1 cut(s) 694
AvaI CYCGRG 1 cut(s) 1185
AvaII GGWCC 2 cut(s) 460, 1300
AxyI CCTNAGG 1 cut(s) 134
BaeI ACNNNNGTAYC 2 cut(s) 1104, 1137
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
BbsI GAAGAC 1 cut(s) 1521
BbvI GCAGC 6 cut(s) 287, 644, 918, 1005, 1380, 1546
BccI CCATC 8 cut(s) 471, 523, 542, 858, 991, 1436, 1813, 1897
BceAI ACGGC 2 cut(s) 141, 919
BciT130I CCWGG 4 cut(s) 361, 611, 1117, 1160
BciVI GTATCC 1 cut(s) 1602
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 192, 1728, 2072
BcuI ACTAGT 1 cut(s) 1396
BfaI CTAG 4 cut(s) 485, 695, 1397, 1700
BfmI CTRYAG 1 cut(s) 812
BfoI RGCGCY 1 cut(s) 1867
BfrI CTTAAG 2 cut(s) 1175, 1925
BfuAI ACCTGC 2 cut(s) 434, 1644
BfuI GTATCC 1 cut(s) 1602
BisI GCNGC 6 cut(s) 301, 633, 907, 1019, 1369, 1535
BlsI GCNGC 6 cut(s) 302, 634, 908, 1020, 1370, 1536
Bme1390I CCNGG 4 cut(s) 361, 611, 1117, 1160
Bme18I GGWCC 2 cut(s) 460, 1300
BmeT110I CYCGRG 1 cut(s) 1185
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 460, 1300
BmiI GGNNCC 1 cut(s) 1753
BmrFI CCNGG 4 cut(s) 361, 611, 1117, 1160
BmrI ACTGGG 2 cut(s) 1058, 1196
BmsI GCATC 7 cut(s) 136, 154, 312, 463, 619, 859, 1799
BmtI GCTAGC 1 cut(s) 698
BmuI ACTGGG 2 cut(s) 1058, 1196
BpiI GAAGAC 1 cut(s) 1521
BpmI CTGGAG 4 cut(s) 134, 343, 987, 1898
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 6 cut(s) 412, 1126, 1730, 1757, 2053, 2092
BsaAI YACGTR 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 609, 1116, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 1602, 1632
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse118I RCCGGY 2 cut(s) 1007, 1754
Bse1I ACTGG 7 cut(s) 117, 749, 1064, 1202, 1610, 1881, 2131
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 2 cut(s) 201, 1990
BseBI CCWGG 4 cut(s) 361, 611, 1117, 1160
BseDI CCNNGG 3 cut(s) 609, 1116, 1360
BseGI GGATG 6 cut(s) 847, 869, 874, 1460, 1669, 1857
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 2 cut(s) 201, 1990
BseMII CTCAG 5 cut(s) 125, 369, 695, 966, 1091
BseNI ACTGG 7 cut(s) 117, 749, 1064, 1202, 1610, 1881, 2131
BseRI GAGGAG 4 cut(s) 120, 835, 1539, 1542
BseXI GCAGC 6 cut(s) 287, 644, 918, 1005, 1380, 1546
BseYI CCCAGC 3 cut(s) 1003, 1387, 2099
BsgI GTGCAG 2 cut(s) 33, 462
BsiHKCI CYCGRG 1 cut(s) 1185
BsiSI CCGG 3 cut(s) 1008, 1755, 1861
BslFI GGGAC 2 cut(s) 1871, 1994
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 3 cut(s) 192, 1728, 2072
BsmFI GGGAC 2 cut(s) 1871, 1994
BsmI GAATGC 2 cut(s) 1180, 1676
BsoBI CYCGRG 1 cut(s) 1185
Bsp119I TTCGAA 1 cut(s) 51
Bsp143I GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
BspACI CCGC 5 cut(s) 5, 167, 397, 698, 1758
BspCNI CTCAG 5 cut(s) 126, 370, 694, 967, 1092
BspHI TCATGA 1 cut(s) 2109
BspLI GGNNCC 1 cut(s) 1753
BspMI ACCTGC 2 cut(s) 434, 1644
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 4 cut(s) 371, 677, 1179, 1661
BspQI GCTCTTC 1 cut(s) 2108
BspT104I TTCGAA 1 cut(s) 51
BspTI CTTAAG 2 cut(s) 1175, 1925
BsrDI GCAATG 2 cut(s) 201, 1990
BsrFI RCCGGY 2 cut(s) 1007, 1754
BsrI ACTGG 7 cut(s) 117, 749, 1064, 1202, 1610, 1881, 2131
BssAI RCCGGY 2 cut(s) 1007, 1754
BssECI CCNNGG 3 cut(s) 609, 1116, 1360
BssMI GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
BssT1I CCWWGG 1 cut(s) 1360
Bst2UI CCWGG 4 cut(s) 361, 611, 1117, 1160
Bst6I CTCTTC 4 cut(s) 150, 812, 2095, 2108
BstAFI CTTAAG 2 cut(s) 1175, 1925
BstBAI YACGTR 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 5 cut(s) 39, 249, 696, 1009, 1756
BstDEI CTNAG 7 cut(s) 101, 134, 194, 378, 681, 975, 1100
BstEII GGTNACC 1 cut(s) 446
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 6 cut(s) 847, 869, 874, 1460, 1669, 1857
BstH2I RGCGCY 1 cut(s) 1867
BstHHI GCGC 6 cut(s) 120, 1018, 1233, 1373, 1866, 2005
BstKTI GATC 6 cut(s) 259, 366, 672, 1174, 1524, 1669
BstMAI GTCTC 3 cut(s) 192, 1728, 2072
BstMBI GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
BstMWI GCNNNNNNNGC 5 cut(s) 11, 34, 523, 941, 1013
BstNI CCWGG 4 cut(s) 361, 611, 1117, 1160
BstNSI RCATGY 1 cut(s) 2009
BstPI GGTNACC 1 cut(s) 446
BstSCI CCNGG 4 cut(s) 359, 609, 1115, 1158
BstSFI CTRYAG 1 cut(s) 812
BstV1I GCAGC 6 cut(s) 287, 644, 918, 1005, 1380, 1546
BstV2I GAAGAC 1 cut(s) 1521
BstX2I RGATCY 1 cut(s) 1171
BstYI RGATCY 1 cut(s) 1171
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 1 cut(s) 1602
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 6 cut(s) 847, 869, 874, 1460, 1669, 1857
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 5 cut(s) 884, 1512, 1603, 1874, 2124
BveI ACCTGC 2 cut(s) 434, 1644
Cac8I GCNNGC 5 cut(s) 39, 249, 696, 1009, 1756
CaiI CAGNNNCTG 3 cut(s) 965, 1159, 1540
CciI TCATGA 1 cut(s) 2109
CfoI GCGC 6 cut(s) 120, 1018, 1233, 1373, 1866, 2005
Cfr10I RCCGGY 2 cut(s) 1007, 1754
Cfr13I GGNCC 2 cut(s) 460, 1300
Csp6I GTAC 4 cut(s) 777, 1044, 1573, 1703
CviAII CATG 8 cut(s) 121, 254, 260, 772, 1973, 1979, 2006, 2110
CviQI GTAC 4 cut(s) 777, 1044, 1573, 1703
DdeI CTNAG 7 cut(s) 101, 134, 194, 378, 681, 975, 1100
DpnI GATC 6 cut(s) 258, 365, 671, 1173, 1523, 1668
DpnII GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
DraI TTTAAA 2 cut(s) 1053, 1092
Eam1104I CTCTTC 4 cut(s) 150, 812, 2095, 2108
EarI CTCTTC 4 cut(s) 150, 812, 2095, 2108
EciI GGCGGA 3 cut(s) 20, 386, 1773
Eco130I CCWWGG 1 cut(s) 1360
Eco32I GATATC 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 460, 1300
Eco57I CTGAAG 4 cut(s) 1136, 1488, 1547, 1667
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 1185
Eco91I GGTNACC 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 1300
EcoO65I GGTNACC 1 cut(s) 446
EcoRII CCWGG 4 cut(s) 359, 609, 1115, 1158
EcoRV GATATC 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 1360
EcoT22I ATGCAT 3 cut(s) 151, 1492, 1814
ErhI CCWWGG 1 cut(s) 1360
FaeI CATG 8 cut(s) 124, 257, 263, 775, 1976, 1982, 2009, 2113
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 1033, 1065
FaqI GGGAC 2 cut(s) 1871, 1994
FatI CATG 8 cut(s) 120, 253, 259, 771, 1972, 1978, 2005, 2109
FauNDI CATATG 2 cut(s) 151, 1288
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 6 cut(s) 301, 633, 907, 1019, 1369, 1535
FokI GGATG 6 cut(s) 854, 876, 881, 1447, 1676, 1844
Fsp4HI GCNGC 6 cut(s) 301, 633, 907, 1019, 1369, 1535
FspBI CTAG 4 cut(s) 485, 695, 1397, 1700
FspI TGCGCA 1 cut(s) 2004
GlaI GCGC 6 cut(s) 119, 1017, 1232, 1372, 1865, 2004
GluI GCNGC 6 cut(s) 301, 633, 907, 1019, 1369, 1535
GsaI CCCAGC 3 cut(s) 1007, 1391, 2103
GsuI CTGGAG 4 cut(s) 134, 343, 987, 1898
HaeII RGCGCY 1 cut(s) 1867
HapII CCGG 3 cut(s) 1008, 1755, 1861
HhaI GCGC 6 cut(s) 120, 1018, 1233, 1373, 1866, 2005
Hin1II CATG 8 cut(s) 124, 257, 263, 775, 1976, 1982, 2009, 2113
Hin6I GCGC 6 cut(s) 118, 1016, 1231, 1371, 1864, 2003
HinP1I GCGC 6 cut(s) 118, 1016, 1231, 1371, 1864, 2003
HindIII AAGCTT 3 cut(s) 35, 290, 1148
HinfI GANTC 4 cut(s) 393, 962, 1078, 1960
HpaII CCGG 3 cut(s) 1008, 1755, 1861
HphI GGTGA 3 cut(s) 482, 1366, 2017
Hpy166II GTNNAC 4 cut(s) 560, 625, 1046, 1357
Hpy188I TCNGA 7 cut(s) 340, 379, 743, 1101, 1155, 1215, 1798
Hpy8I GTNNAC 4 cut(s) 560, 625, 1046, 1357
HpyAV CCTTC 6 cut(s) 251, 1340, 1404, 1623, 1691, 1724
HpyCH4IV ACGT 2 cut(s) 562, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 34, 523, 941, 1013
HpyF3I CTNAG 7 cut(s) 101, 134, 194, 378, 681, 975, 1100
HpySE526I ACGT 2 cut(s) 562, 661
Hsp92II CATG 8 cut(s) 124, 257, 263, 775, 1976, 1982, 2009, 2113
HspAI GCGC 6 cut(s) 118, 1016, 1231, 1371, 1864, 2003
KroI GCCGGC 2 cut(s) 1007, 1754
KroNI GCCGGC 2 cut(s) 1009, 1756
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
LguI GCTCTTC 1 cut(s) 2108
LmnI GCTCC 6 cut(s) 115, 437, 732, 1316, 1650, 1751
Lsp1109I GCAGC 6 cut(s) 287, 644, 918, 1005, 1380, 1546
LweI GCATC 7 cut(s) 136, 154, 312, 463, 619, 859, 1799
MaeI CTAG 4 cut(s) 485, 695, 1397, 1700
MaeII ACGT 2 cut(s) 562, 661
MaeIII GTNAC 4 cut(s) 342, 446, 1189, 2023
MalI GATC 6 cut(s) 258, 365, 671, 1173, 1523, 1668
MboI GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
MflI RGATCY 1 cut(s) 1171
MluCI AATT 7 cut(s) 90, 233, 330, 404, 656, 798, 1209
MlyI GAGTC 2 cut(s) 387, 1954
MmeI TCCRAC 3 cut(s) 435, 1246, 2111
Mph1103I ATGCAT 3 cut(s) 151, 1492, 1814
MroNI GCCGGC 2 cut(s) 1007, 1754
MroXI GAANNNNTTC 4 cut(s) 222, 334, 650, 1275
MslI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1352, 1817, 1977, 1983
MspA1I CMGCKG 1 cut(s) 787
MspCI CTTAAG 2 cut(s) 1175, 1925
MspI CCGG 3 cut(s) 1008, 1755, 1861
MspR9I CCNGG 4 cut(s) 361, 611, 1117, 1160
Mva1269I GAATGC 2 cut(s) 1180, 1676
MvaI CCWGG 4 cut(s) 361, 611, 1117, 1160
MwoI GCNNNNNNNGC 5 cut(s) 11, 34, 523, 941, 1013
NaeI GCCGGC 2 cut(s) 1009, 1756
NdeI CATATG 2 cut(s) 151, 1288
NdeII GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
NgoMIV GCCGGC 2 cut(s) 1007, 1754
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 8 cut(s) 124, 257, 263, 775, 1976, 1982, 2009, 2113
NlaIV GGNNCC 1 cut(s) 1753
NmuCI GTSAC 1 cut(s) 2023
NsbI TGCGCA 1 cut(s) 2004
NsiI ATGCAT 3 cut(s) 151, 1492, 1814
NspI RCATGY 1 cut(s) 2009
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 213
PaeR7I CTCGAG 1 cut(s) 1185
PagI TCATGA 1 cut(s) 2109
PciSI GCTCTTC 1 cut(s) 2108
PctI GAATGC 2 cut(s) 1180, 1676
PdiI GCCGGC 2 cut(s) 1009, 1756
PdmI GAANNNNTTC 4 cut(s) 222, 334, 650, 1275
PfeI GAWTC 2 cut(s) 962, 1078
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 6 cut(s) 302, 634, 908, 1020, 1370, 1536
PleI GAGTC 2 cut(s) 387, 1954
PpsI GAGTC 2 cut(s) 387, 1954
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 1300
Psp5II RGGWCCY 1 cut(s) 1300
Psp6I CCWGG 4 cut(s) 359, 609, 1115, 1158
PspEI GGTNACC 1 cut(s) 446
PspFI CCCAGC 3 cut(s) 1003, 1387, 2099
PspGI CCWGG 4 cut(s) 359, 609, 1115, 1158
PspN4I GGNNCC 1 cut(s) 1753
PspPI GGNCC 2 cut(s) 460, 1300
PspPPI RGGWCCY 1 cut(s) 1300
PstNI CAGNNNCTG 3 cut(s) 965, 1159, 1540
PsuI RGATCY 1 cut(s) 1171
PvuII CAGCTG 1 cut(s) 787
RsaI GTAC 4 cut(s) 778, 1045, 1574, 1704
RsaNI GTAC 4 cut(s) 777, 1044, 1573, 1703
RseI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1352, 1817, 1977, 1983
SapI GCTCTTC 1 cut(s) 2108
SatI GCNGC 6 cut(s) 301, 633, 907, 1019, 1369, 1535
Sau3AI GATC 6 cut(s) 256, 363, 669, 1171, 1521, 1666
Sau96I GGNCC 2 cut(s) 460, 1300
SchI GAGTC 2 cut(s) 387, 1954
ScrFI CCNGG 4 cut(s) 361, 611, 1117, 1160
SfaNI GCATC 7 cut(s) 136, 154, 312, 463, 619, 859, 1799
SfcI CTRYAG 1 cut(s) 812
Sfr274I CTCGAG 1 cut(s) 1185
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 2 cut(s) 460, 1300
SlaI CTCGAG 1 cut(s) 1185
SmiMI CAYNNNNRTG 8 cut(s) 213, 258, 468, 858, 1352, 1817, 1977, 1983
SmlI CTYRAG 9 cut(s) 427, 1105, 1175, 1185, 1709, 1772, 1925, 2032, 2071
SmoI CTYRAG 9 cut(s) 427, 1105, 1175, 1185, 1709, 1772, 1925, 2032, 2071
SpeI ACTAGT 1 cut(s) 1396
Sse9I AATT 7 cut(s) 90, 233, 330, 404, 656, 798, 1209
SsiI CCGC 5 cut(s) 5, 167, 397, 698, 1758
SspI AATATT 1 cut(s) 898
SspMI CTAG 4 cut(s) 485, 695, 1397, 1700
StyD4I CCNGG 4 cut(s) 359, 609, 1115, 1158
StyI CCWWGG 1 cut(s) 1360
TaiI ACGT 2 cut(s) 565, 664
TaqI TCGA 8 cut(s) 51, 322, 328, 466, 928, 1186, 1788, 1807
TasI AATT 7 cut(s) 90, 233, 330, 404, 656, 798, 1209
TatI WGTACW 3 cut(s) 1043, 1572, 1702
TfiI GAWTC 2 cut(s) 962, 1078
TscAI CASTG 5 cut(s) 891, 1512, 1610, 1881, 2131
TseFI GTSAC 1 cut(s) 2023
TseI GCWGC 6 cut(s) 300, 632, 906, 1018, 1368, 1534
Tsp45I GTSAC 1 cut(s) 2023
TspDTI ATGAA 7 cut(s) 168, 246, 374, 1336, 1449, 1815, 2126
TspRI CASTG 5 cut(s) 891, 1512, 1610, 1881, 2131
Vha464I CTTAAG 2 cut(s) 1175, 1925
VpaK11BI GGWCC 2 cut(s) 460, 1300
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 4 cut(s) 90, 233, 404, 1209
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 1 cut(s) 2009
XcmI CCANNNNNNNNNTGG 1 cut(s) 1504
XhoI CTCGAG 1 cut(s) 1185
XmiI GTMKAC 1 cut(s) 624
XmnI GAANNNNTTC 4 cut(s) 222, 334, 650, 1275
XspI CTAG 4 cut(s) 485, 695, 1397, 1700
Zsp2I ATGCAT 3 cut(s) 151, 1492, 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.