RLG00000018806

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000888
Physical Location & Seq
Forward (+)
36539080 .. 36541780
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018806

Sequence Viewer

Length: 2526 bp
ATGGCGGACAGTGCAGTAAAGTTTCTACTTGATAAGCTTGCACCTCTTTTCGAAAATGAGTTGCAACTTTTGAGGGGGGTTAGGGAAGAAATTTTGCATCTAAGAGGAGAACTGGAGCGCATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAGCTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGAGTTACGAGCCATAAACTCCAGGATCAAAAAAATCTCTGAGGAGGACCATCGAGGTGATGCTTTACTTCTAGAGAAAACTGATATAGTGGGCATAGACGAGCCTATTAAGCAGATGGTTGGGAGGCTGGTCAATGGTGGTTGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGGGAAAACTACCCTGGCGAAGCAAGTCTATGATGCAGCAGAAGTGAAGAAACATTTCAAATTACGTGCTAGCGGAACTTTTACTCAGTCTTTTAGGCTAGCGGAACTTCTCAAAGACATCAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGTATGCACTGCCTATTTATATTTACGGCAGCAGAGTGATACTAACTACTCGAAATGCAGATATAGCCTCCACAACAAGCACGGAATCTGGAGGTGAAGTCTATACTTTGAAGCCCTTGCCCCTAGTGGAGTCATGGGAGCTCCTGTGCAAGAAGACATTCCAAGGGAATTCCTGCCCTCCTCATTTAGAGAAAATCTGTAACTATGTTCTGAGAAAGTGTGAGGGGCTACCCCTTGCTATTGTGGCAATTAGTGGAGTTCTGGCTGCTAAAGATAAGCGCAGAATTGATGAGTGGGAAATGGTTGGCCACAGTCTCGGGGCTGAAATTGAGGGTAATGACAAACTGCAGGACTTGAAGAAAGTACTTTCACTCAGTTTCAATGATTTACCATACTATCTAAAATCGTGCTTCTTGTACTTGAGCATCTTTCCAGAGGACCATCTAATTAAGCATATGAGGCTTATTCGATTATGGATGGCAGAGGGCTTCATTGAAGAAAAACAAGGAAAGACATTGGAAGAGGTTGCAGAGGATTACCTCAATGAACTATTGAATAGGAGCATGATTCAAGCAGCAGAGACAACAGCTGATGGAAGGGTCACAAAATTTCGTATCCATGATCTCTTCTGCGAAATTATCACCTCCAAGACAAGAAGTCAGAACTTTGCCACAATAGCCAAAGAGCAGAATGTAACATTGCCAGAAAAAATCCGTCGCCTGTCAATACATAACACACTGCAATATGTTCAGACAAACAAGTGTGCTTCTCAACTACGTTCTCTTTTTATGTTCACAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGCGCTGCTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCAGTTGAAGTTGTGAATCTTTACTTTTTAAAGTATCTGAGCTTGAGAGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCAAGTTACTGAACTCCCAGTTGAAATTCTGAAACTCCAACATCTGCCCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTTTCACTCAACATGTGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAACGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGCAGCACCTGCGTTCTCCTCCTCTATTGCTTCAGCATTTGTACTTGCGAGGCAGACTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTCGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTGCCCAATCTAGTACATCTTGAGTTGTGTCAAGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAAGAAGCTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCTTGTATTGAAAATCTAATCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCACGCATTGAACATCTCATCAAACTAAAGTTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

842

Amino Acids

96.27

Weight (kDa)

8.56

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 2.6e-23 Rx N-terminal domain
NB-ARC PF00931 147 - 214 1.9e-10 NB-ARC domain
NB-ARC PF00931 209 - 292 4.7e-15 NB-ARC domain
WHD_DRP PF23559 377 - 448 2.8e-26 Disease resistance protein Winged helix domain
LRR_14 PF23598 492 - 817 2.6e-44 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2079
Acc36I ACCTGC 2 cut(s) 2079, 2175
AciI CCGC 5 cut(s) 5, 167, 594, 623, 2289
AclWI GGATC 3 cut(s) 371, 1710, 2192
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 5 cut(s) 90, 233, 871, 1310, 1740
AcuI CTGAAG 4 cut(s) 1667, 2019, 2078, 2198
AfaI GTAC 5 cut(s) 1068, 1121, 1576, 2105, 2235
AflII CTTAAG 2 cut(s) 1706, 2456
AflIII ACRYGT 1 cut(s) 1817
AhdI GACNNNNNGTC 1 cut(s) 1359
AhlI ACTAGT 1 cut(s) 1927
AjiI CACGTC 1 cut(s) 488
AjnI CCWGG 4 cut(s) 359, 534, 1646, 1689
AleI CACNNNNGTG 1 cut(s) 213
Alw21I GWGCWC 2 cut(s) 174, 846
Alw26I GTCTC 4 cut(s) 192, 1022, 1277, 2259
AlwI GGATC 3 cut(s) 371, 1710, 2192
AlwNI CAGNNNCTG 3 cut(s) 1505, 1690, 2071
Ama87I CYCGRG 1 cut(s) 1019
AoxI GGCC 1 cut(s) 1009
ApeKI GCWGC 7 cut(s) 300, 557, 732, 968, 1277, 1549, 2065
ApoI RAATTY 5 cut(s) 90, 233, 871, 1310, 1740
Asp700I GAANNNNTTC 5 cut(s) 222, 334, 575, 860, 1806
AspLEI GCGC 4 cut(s) 120, 984, 1549, 2397
AspS9I GGNCC 3 cut(s) 385, 1141, 1831
AsuHPI GGTGA 4 cut(s) 407, 809, 1336, 1897
AsuII TTCGAA 1 cut(s) 51
AsuNHI GCTAGC 2 cut(s) 590, 619
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 3 cut(s) 385, 1141, 1831
AxyI CCTNAGG 1 cut(s) 134
BaeI ACNNNNGTAYC 2 cut(s) 1635, 1668
BalI TGGCCA 1 cut(s) 1011
BanII GRGCYC 2 cut(s) 174, 846
BbsI GAAGAC 2 cut(s) 863, 2052
Bbv12I GWGCWC 2 cut(s) 174, 846
BbvI GCAGC 7 cut(s) 287, 569, 744, 955, 1289, 1536, 2077
BceAI ACGGC 2 cut(s) 141, 745
BciT130I CCWGG 4 cut(s) 361, 536, 1648, 1691
BciVI GTATCC 2 cut(s) 1328, 2133
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 4 cut(s) 192, 1022, 1277, 2259
BcuI ACTAGT 1 cut(s) 1927
BfaI CTAG 6 cut(s) 410, 591, 620, 827, 1928, 2231
BfmI CTRYAG 1 cut(s) 1049
BfoI RGCGCY 1 cut(s) 2398
BfrI CTTAAG 2 cut(s) 1706, 2456
BfuAI ACCTGC 2 cut(s) 2079, 2175
BfuI GTATCC 2 cut(s) 1328, 2133
BisI GCNGC 7 cut(s) 301, 558, 733, 969, 1278, 1550, 2066
BlsI GCNGC 7 cut(s) 302, 559, 734, 970, 1279, 1551, 2067
BmcAI AGTACT 1 cut(s) 1068
Bme1390I CCNGG 4 cut(s) 361, 536, 1648, 1691
Bme18I GGWCC 3 cut(s) 385, 1141, 1831
BmeRI GACNNNNNGTC 1 cut(s) 1359
BmeT110I CYCGRG 1 cut(s) 1019
BmgBI CACGTC 1 cut(s) 488
BmgT120I GGNCC 3 cut(s) 385, 1141, 1831
BmiI GGNNCC 1 cut(s) 2284
BmrFI CCNGG 4 cut(s) 361, 536, 1648, 1691
BmrI ACTGGG 2 cut(s) 1589, 1727
BmsI GCATC 9 cut(s) 106, 136, 154, 312, 388, 544, 685, 1137, 2330
BmtI GCTAGC 2 cut(s) 594, 623
BmuI ACTGGG 2 cut(s) 1589, 1727
BpiI GAAGAC 2 cut(s) 863, 2052
BpmI CTGGAG 4 cut(s) 134, 343, 813, 2429
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 5 cut(s) 1144, 1657, 1701, 2261, 2288
BsaAI YACGTR 1 cut(s) 587
BsaJI CCNNGG 4 cut(s) 534, 865, 1647, 1891
BsaXI ACNNNNNCTCC 6 cut(s) 786, 816, 833, 863, 2133, 2163
Bse118I RCCGGY 2 cut(s) 1538, 2285
Bse1I ACTGG 6 cut(s) 117, 1595, 1733, 2124, 2141, 2412
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 3 cut(s) 201, 1400, 2521
BseBI CCWGG 4 cut(s) 361, 536, 1648, 1691
BseDI CCNNGG 4 cut(s) 534, 865, 1647, 1891
BseGI GGATG 7 cut(s) 673, 695, 700, 1185, 1991, 2200, 2388
BseMI GCAATG 3 cut(s) 201, 1400, 2521
BseMII CTCAG 7 cut(s) 125, 369, 620, 905, 1090, 1497, 1622
BseNI ACTGG 6 cut(s) 117, 1595, 1733, 2124, 2141, 2412
BseRI GAGGAG 6 cut(s) 120, 395, 661, 873, 2070, 2073
BseXI GCAGC 7 cut(s) 287, 569, 744, 955, 1289, 1536, 2077
BseYI CCCAGC 2 cut(s) 1534, 1918
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 1 cut(s) 1011
BsiHKAI GWGCWC 2 cut(s) 174, 846
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 3 cut(s) 1539, 2286, 2392
BslFI GGGAC 1 cut(s) 2402
BsmAI GTCTC 4 cut(s) 192, 1022, 1277, 2259
BsmFI GGGAC 1 cut(s) 2402
BsmI GAATGC 2 cut(s) 1711, 2207
BsnI GGCC 1 cut(s) 1011
BsoBI CYCGRG 1 cut(s) 1019
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 174, 846
Bsp143I GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
BspACI CCGC 5 cut(s) 5, 167, 594, 623, 2289
BspANI GGCC 1 cut(s) 1011
BspCNI CTCAG 7 cut(s) 126, 370, 619, 906, 1089, 1498, 1623
BspLI GGNNCC 1 cut(s) 2284
BspMAI CTGCAG 1 cut(s) 1053
BspMI ACCTGC 2 cut(s) 2079, 2175
BspOI GCTAGC 2 cut(s) 594, 623
BspPI GGATC 3 cut(s) 371, 1710, 2192
BspT104I TTCGAA 1 cut(s) 51
BspTI CTTAAG 2 cut(s) 1706, 2456
BsrDI GCAATG 3 cut(s) 201, 1400, 2521
BsrFI RCCGGY 2 cut(s) 1538, 2285
BsrI ACTGG 6 cut(s) 117, 1595, 1733, 2124, 2141, 2412
BssAI RCCGGY 2 cut(s) 1538, 2285
BssECI CCNNGG 4 cut(s) 534, 865, 1647, 1891
BssMI GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
BssT1I CCWWGG 2 cut(s) 865, 1891
Bst2UI CCWGG 4 cut(s) 361, 536, 1648, 1691
Bst4CI ACNGT 3 cut(s) 11, 1016, 1501
Bst6I CTCTTC 3 cut(s) 150, 1218, 1334
BstAFI CTTAAG 2 cut(s) 1706, 2456
BstAPI GCANNNNNTGC 1 cut(s) 2071
BstBAI YACGTR 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 6 cut(s) 39, 249, 592, 621, 1540, 2287
BstDEI CTNAG 9 cut(s) 101, 134, 194, 378, 606, 914, 1076, 1506, 1631
BstF5I GGATG 7 cut(s) 673, 695, 700, 1185, 1991, 2200, 2388
BstH2I RGCGCY 1 cut(s) 2398
BstHHI GCGC 4 cut(s) 120, 984, 1549, 2397
BstKTI GATC 6 cut(s) 259, 366, 1327, 1705, 2055, 2200
BstMAI GTCTC 4 cut(s) 192, 1022, 1277, 2259
BstMBI GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
BstMWI GCNNNNNNNGC 8 cut(s) 11, 448, 767, 947, 1162, 1379, 1544, 2071
BstNI CCWGG 4 cut(s) 361, 536, 1648, 1691
BstNSI RCATGY 1 cut(s) 1821
BstSCI CCNGG 4 cut(s) 359, 534, 1646, 1689
BstSFI CTRYAG 1 cut(s) 1049
BstV1I GCAGC 7 cut(s) 287, 569, 744, 955, 1289, 1536, 2077
BstV2I GAAGAC 2 cut(s) 863, 2052
BstX2I RGATCY 1 cut(s) 1702
BstYI RGATCY 1 cut(s) 1702
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 2 cut(s) 1328, 2133
BsuRI GGCC 1 cut(s) 1011
BtrI CACGTC 1 cut(s) 488
BtsCI GGATG 7 cut(s) 673, 695, 700, 1185, 1991, 2200, 2388
BtsI GCAGTG 2 cut(s) 710, 1439
BtsIMutI CAGTG 6 cut(s) 16, 710, 1439, 2043, 2134, 2405
BveI ACCTGC 2 cut(s) 2079, 2175
Cac8I GCNNGC 6 cut(s) 39, 249, 592, 621, 1540, 2287
CaiI CAGNNNCTG 3 cut(s) 1505, 1690, 2071
CfoI GCGC 4 cut(s) 120, 984, 1549, 2397
Cfr10I RCCGGY 2 cut(s) 1538, 2285
Cfr13I GGNCC 3 cut(s) 385, 1141, 1831
Csp6I GTAC 5 cut(s) 1067, 1120, 1575, 2104, 2234
CviAII CATG 9 cut(s) 121, 254, 260, 837, 1267, 1322, 1818, 2504, 2510
CviQI GTAC 5 cut(s) 1067, 1120, 1575, 2104, 2234
DdeI CTNAG 9 cut(s) 101, 134, 194, 378, 606, 914, 1076, 1506, 1631
DpnI GATC 6 cut(s) 258, 365, 1326, 1704, 2054, 2199
DpnII GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
DraI TTTAAA 2 cut(s) 1584, 1623
DriI GACNNNNNGTC 1 cut(s) 1359
EaeI YGGCCR 1 cut(s) 1009
Eam1104I CTCTTC 3 cut(s) 150, 1218, 1334
Eam1105I GACNNNNNGTC 1 cut(s) 1359
EarI CTCTTC 3 cut(s) 150, 1218, 1334
EciI GGCGGA 2 cut(s) 20, 2304
Ecl136II GAGCTC 2 cut(s) 172, 844
Eco130I CCWWGG 2 cut(s) 865, 1891
Eco24I GRGCYC 2 cut(s) 174, 846
Eco32I GATATC 1 cut(s) 326
Eco47I GGWCC 3 cut(s) 385, 1141, 1831
Eco53kI GAGCTC 2 cut(s) 172, 844
Eco57I CTGAAG 4 cut(s) 1667, 2019, 2078, 2198
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 1019
EcoICRI GAGCTC 2 cut(s) 172, 844
EcoO109I RGGNCCY 1 cut(s) 1831
EcoRI GAATTC 1 cut(s) 871
EcoRII CCWGG 4 cut(s) 359, 534, 1646, 1689
EcoRV GATATC 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 865, 1891
EcoT22I ATGCAT 3 cut(s) 151, 2023, 2345
EcoT38I GRGCYC 2 cut(s) 174, 846
ErhI CCWWGG 2 cut(s) 865, 1891
FaeI CATG 9 cut(s) 124, 257, 263, 840, 1270, 1325, 1821, 2507, 2513
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 1564, 1596
FaqI GGGAC 1 cut(s) 2402
FatI CATG 9 cut(s) 120, 253, 259, 836, 1266, 1321, 1817, 2503, 2509
FauNDI CATATG 2 cut(s) 151, 1158
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 7 cut(s) 301, 558, 733, 969, 1278, 1550, 2066
FokI GGATG 7 cut(s) 680, 702, 707, 1192, 1978, 2207, 2375
FriOI GRGCYC 2 cut(s) 174, 846
Fsp4HI GCNGC 7 cut(s) 301, 558, 733, 969, 1278, 1550, 2066
FspBI CTAG 6 cut(s) 410, 591, 620, 827, 1928, 2231
GlaI GCGC 4 cut(s) 119, 983, 1548, 2396
GluI GCNGC 7 cut(s) 301, 558, 733, 969, 1278, 1550, 2066
GsaI CCCAGC 2 cut(s) 1538, 1922
GsuI CTGGAG 4 cut(s) 134, 343, 813, 2429
HaeII RGCGCY 1 cut(s) 2398
HaeIII GGCC 1 cut(s) 1011
HapII CCGG 3 cut(s) 1539, 2286, 2392
HhaI GCGC 4 cut(s) 120, 984, 1549, 2397
Hin1II CATG 9 cut(s) 124, 257, 263, 840, 1270, 1325, 1821, 2507, 2513
Hin6I GCGC 4 cut(s) 118, 982, 1547, 2395
HinP1I GCGC 4 cut(s) 118, 982, 1547, 2395
HindIII AAGCTT 3 cut(s) 35, 290, 1679
HinfI GANTC 5 cut(s) 788, 833, 1270, 1609, 2491
HpaII CCGG 3 cut(s) 1539, 2286, 2392
HphI GGTGA 4 cut(s) 407, 809, 1336, 1897
Hpy166II GTNNAC 4 cut(s) 485, 1497, 1577, 1888
Hpy188I TCNGA 9 cut(s) 340, 379, 915, 1365, 1455, 1632, 1686, 1746, 2329
Hpy8I GTNNAC 4 cut(s) 485, 1497, 1577, 1888
Hpy99I CGWCG 1 cut(s) 1422
HpyAV CCTTC 7 cut(s) 251, 1293, 1871, 1935, 2154, 2222, 2255
HpyCH4III ACNGT 3 cut(s) 11, 1016, 1501
HpyCH4IV ACGT 3 cut(s) 487, 586, 1480
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 448, 767, 947, 1162, 1379, 1544, 2071
HpyF3I CTNAG 9 cut(s) 101, 134, 194, 378, 606, 914, 1076, 1506, 1631
HpySE526I ACGT 3 cut(s) 487, 586, 1480
Hsp92II CATG 9 cut(s) 124, 257, 263, 840, 1270, 1325, 1821, 2507, 2513
HspAI GCGC 4 cut(s) 118, 982, 1547, 2395
KroI GCCGGC 2 cut(s) 1538, 2285
KroNI GCCGGC 2 cut(s) 1540, 2287
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
LmnI GCTCC 8 cut(s) 115, 169, 841, 849, 1263, 1847, 2181, 2282
Lsp1109I GCAGC 7 cut(s) 287, 569, 744, 955, 1289, 1536, 2077
LweI GCATC 9 cut(s) 106, 136, 154, 312, 388, 544, 685, 1137, 2330
MaeI CTAG 6 cut(s) 410, 591, 620, 827, 1928, 2231
MaeII ACGT 3 cut(s) 487, 586, 1480
MaeIII GTNAC 5 cut(s) 342, 902, 1303, 1396, 1720
MalI GATC 6 cut(s) 258, 365, 1326, 1704, 2054, 2199
MboI GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
MflI RGATCY 1 cut(s) 1702
MhlI GDGCHC 2 cut(s) 174, 846
MlsI TGGCCA 1 cut(s) 1011
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 842, 2485
MmeI TCCRAC 1 cut(s) 1777
Mox20I TGGCCA 1 cut(s) 1011
Mph1103I ATGCAT 3 cut(s) 151, 2023, 2345
MroNI GCCGGC 2 cut(s) 1538, 2285
MroXI GAANNNNTTC 5 cut(s) 222, 334, 575, 860, 1806
MscI TGGCCA 1 cut(s) 1011
MslI CAYNNNNRTG 8 cut(s) 213, 258, 393, 684, 1883, 2348, 2508, 2514
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 1292
MspCI CTTAAG 2 cut(s) 1706, 2456
MspI CCGG 3 cut(s) 1539, 2286, 2392
MspR9I CCNGG 4 cut(s) 361, 536, 1648, 1691
Mva1269I GAATGC 2 cut(s) 1711, 2207
MvaI CCWGG 4 cut(s) 361, 536, 1648, 1691
MwoI GCNNNNNNNGC 8 cut(s) 11, 448, 767, 947, 1162, 1379, 1544, 2071
NaeI GCCGGC 2 cut(s) 1540, 2287
NdeI CATATG 2 cut(s) 151, 1158
NdeII GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
NgoMIV GCCGGC 2 cut(s) 1538, 2285
NheI GCTAGC 2 cut(s) 590, 619
NlaIII CATG 9 cut(s) 124, 257, 263, 840, 1270, 1325, 1821, 2507, 2513
NlaIV GGNNCC 1 cut(s) 2284
NmuCI GTSAC 1 cut(s) 1303
NsiI ATGCAT 3 cut(s) 151, 2023, 2345
NspI RCATGY 1 cut(s) 1821
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 213
PaqCI CACCTGC 1 cut(s) 2079
PciI ACATGT 1 cut(s) 1817
PctI GAATGC 2 cut(s) 1711, 2207
PdiI GCCGGC 2 cut(s) 1540, 2287
PdmI GAANNNNTTC 5 cut(s) 222, 334, 575, 860, 1806
PfeI GAWTC 3 cut(s) 788, 1270, 1609
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 7 cut(s) 302, 559, 734, 970, 1279, 1551, 2067
PleI GAGTC 2 cut(s) 841, 2485
PpsI GAGTC 2 cut(s) 841, 2485
Ppu21I YACGTR 1 cut(s) 587
PpuMI RGGWCCY 1 cut(s) 1831
PscI ACATGT 1 cut(s) 1817
Psp124BI GAGCTC 2 cut(s) 174, 846
Psp5II RGGWCCY 1 cut(s) 1831
Psp6I CCWGG 4 cut(s) 359, 534, 1646, 1689
PspFI CCCAGC 2 cut(s) 1534, 1918
PspGI CCWGG 4 cut(s) 359, 534, 1646, 1689
PspN4I GGNNCC 1 cut(s) 2284
PspPI GGNCC 3 cut(s) 385, 1141, 1831
PspPPI RGGWCCY 1 cut(s) 1831
PstI CTGCAG 1 cut(s) 1053
PstNI CAGNNNCTG 3 cut(s) 1505, 1690, 2071
PsuI RGATCY 1 cut(s) 1702
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 5 cut(s) 1068, 1121, 1576, 2105, 2235
RsaNI GTAC 5 cut(s) 1067, 1120, 1575, 2104, 2234
RseI CAYNNNNRTG 8 cut(s) 213, 258, 393, 684, 1883, 2348, 2508, 2514
SacI GAGCTC 2 cut(s) 174, 846
SatI GCNGC 7 cut(s) 301, 558, 733, 969, 1278, 1550, 2066
Sau3AI GATC 6 cut(s) 256, 363, 1324, 1702, 2052, 2197
Sau96I GGNCC 3 cut(s) 385, 1141, 1831
ScaI AGTACT 1 cut(s) 1068
SchI GAGTC 2 cut(s) 842, 2485
ScrFI CCNGG 4 cut(s) 361, 536, 1648, 1691
SduI GDGCHC 2 cut(s) 174, 846
SfaNI GCATC 9 cut(s) 106, 136, 154, 312, 388, 544, 685, 1137, 2330
SfcI CTRYAG 1 cut(s) 1049
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 3 cut(s) 385, 1141, 1831
SmiMI CAYNNNNRTG 8 cut(s) 213, 258, 393, 684, 1883, 2348, 2508, 2514
SmlI CTYRAG 7 cut(s) 1123, 1636, 1706, 1716, 2240, 2303, 2456
SmoI CTYRAG 7 cut(s) 1123, 1636, 1706, 1716, 2240, 2303, 2456
SpeI ACTAGT 1 cut(s) 1927
SsiI CCGC 5 cut(s) 5, 167, 594, 623, 2289
SspMI CTAG 6 cut(s) 410, 591, 620, 827, 1928, 2231
SstI GAGCTC 2 cut(s) 174, 846
StyD4I CCNGG 4 cut(s) 359, 534, 1646, 1689
StyI CCWWGG 2 cut(s) 865, 1891
TaaI ACNGT 3 cut(s) 11, 1016, 1501
TaiI ACGT 3 cut(s) 490, 589, 1483
TaqI TCGA 8 cut(s) 51, 322, 328, 391, 754, 1171, 2319, 2338
TatI WGTACW 5 cut(s) 1066, 1119, 1574, 2103, 2233
TfiI GAWTC 3 cut(s) 788, 1270, 1609
TscAI CASTG 6 cut(s) 16, 717, 1446, 2043, 2141, 2412
TseFI GTSAC 1 cut(s) 1303
TseI GCWGC 7 cut(s) 300, 557, 732, 968, 1277, 1549, 2065
Tsp45I GTSAC 1 cut(s) 1303
TspDTI ATGAA 7 cut(s) 168, 246, 1183, 1263, 1867, 1980, 2346
TspGWI ACGGA 2 cut(s) 800, 1406
TspRI CASTG 6 cut(s) 16, 717, 1446, 2043, 2141, 2412
Vha464I CTTAAG 2 cut(s) 1706, 2456
VpaK11BI GGWCC 3 cut(s) 385, 1141, 1831
XapI RAATTY 5 cut(s) 90, 233, 871, 1310, 1740
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 1 cut(s) 1821
XcmI CCANNNNNNNNNTGG 1 cut(s) 2035
XmnI GAANNNNTTC 5 cut(s) 222, 334, 575, 860, 1806
XspI CTAG 6 cut(s) 410, 591, 620, 827, 1928, 2231
ZrmI AGTACT 1 cut(s) 1068
Zsp2I ATGCAT 3 cut(s) 151, 2023, 2345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.