Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 291 |
| Acc65I | GGTACC | 1 cut(s) | 822 |
| AccB1I | GGYRCC | 1 cut(s) | 822 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1945 |
| AccI | GTMKAC | 2 cut(s) | 1159, 2754 |
| AciI | CCGC | 5 cut(s) | 298, 948, 1713, 1813, 2272 |
| AclWI | GGATC | 5 cut(s) | 623, 674, 1023, 1336, 2366 |
| AcoI | YGGCCR | 3 cut(s) | 556, 609, 2071 |
| AcsI | RAATTY | 4 cut(s) | 282, 689, 1071, 1763 |
| AcuI | CTGAAG | 5 cut(s) | 987, 1702, 2193, 2252, 2437 |
| AcyI | GRCGYC | 1 cut(s) | 1594 |
| AfaI | GTAC | 7 cut(s) | 824, 960, 1242, 1271, 1295, 2279, 2786 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 135, 304, 481, 1011, 1351, 1473, 1945, 2018, 2085 |
| AflII | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| AflIII | ACRYGT | 3 cut(s) | 768, 1154, 1484 |
| AgeI | ACCGGT | 1 cut(s) | 678 |
| AjiI | CACGTC | 1 cut(s) | 148 |
| AjnI | CCWGG | 3 cut(s) | 1419, 2490, 2580 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 590, 622 |
| Alw21I | GWGCWC | 1 cut(s) | 66 |
| Alw26I | GTCTC | 6 cut(s) | 192, 397, 702, 1330, 2349, 2433 |
| Alw44I | GTGCAC | 1 cut(s) | 62 |
| AlwI | GGATC | 5 cut(s) | 623, 674, 1023, 1336, 2366 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2275 |
| Ama87I | CYCGRG | 1 cut(s) | 2630 |
| AoxI | GGCC | 7 cut(s) | 126, 502, 556, 609, 975, 2071, 2122 |
| ApaLI | GTGCAC | 1 cut(s) | 62 |
| ApeKI | GCWGC | 6 cut(s) | 151, 808, 903, 950, 1723, 1933 |
| ApoI | RAATTY | 4 cut(s) | 282, 689, 1071, 1763 |
| AseI | ATTAAT | 1 cut(s) | 2420 |
| AsiGI | ACCGGT | 1 cut(s) | 678 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 647, 1833, 1980, 2421 |
| Asp718I | GGTACC | 1 cut(s) | 822 |
| AspA2I | CCTAGG | 1 cut(s) | 73 |
| AspLEI | GCGC | 2 cut(s) | 1332, 1938 |
| AspS9I | GGNCC | 1 cut(s) | 1315 |
| AsuC2I | CCSGG | 1 cut(s) | 2711 |
| AsuHPI | GGTGA | 5 cut(s) | 196, 401, 772, 1537, 1743 |
| AsuII | TTCGAA | 1 cut(s) | 51 |
| AvaI | CYCGRG | 1 cut(s) | 2630 |
| AvaII | GGWCC | 1 cut(s) | 1315 |
| AvrII | CCTAGG | 1 cut(s) | 73 |
| AxyI | CCTNAGG | 1 cut(s) | 1311 |
| BaeGI | GKGCMC | 2 cut(s) | 66, 2197 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 806, 839 |
| BalI | TGGCCA | 1 cut(s) | 611 |
| BanI | GGYRCC | 1 cut(s) | 822 |
| BanII | GRGCYC | 1 cut(s) | 516 |
| BbsI | GAAGAC | 1 cut(s) | 2226 |
| Bbv12I | GWGCWC | 1 cut(s) | 66 |
| BbvCI | CCTCAGC | 2 cut(s) | 216, 1250 |
| BbvI | GCAGC | 6 cut(s) | 138, 795, 915, 937, 1710, 1920 |
| BceAI | ACGGC | 2 cut(s) | 543, 1252 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 443, 477, 848, 882 |
| BciT130I | CCWGG | 3 cut(s) | 1421, 2492, 2582 |
| BclI | TGATCA | 3 cut(s) | 256, 262, 268 |
| BcnI | CCSGG | 1 cut(s) | 2711 |
| BcoDI | GTCTC | 6 cut(s) | 192, 397, 702, 1330, 2349, 2433 |
| BfaI | CTAG | 6 cut(s) | 74, 227, 1698, 1865, 1874, 2333 |
| BfmI | CTRYAG | 6 cut(s) | 141, 152, 809, 1024, 2036, 2202 |
| BfrI | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| BfuAI | ACCTGC | 1 cut(s) | 291 |
| BglII | AGATCT | 1 cut(s) | 1841 |
| BisI | GCNGC | 8 cut(s) | 152, 809, 904, 951, 1714, 1724, 1934, 2273 |
| BlnI | CCTAGG | 1 cut(s) | 73 |
| BlsI | GCNGC | 8 cut(s) | 153, 810, 905, 952, 1715, 1725, 1935, 2274 |
| BmcAI | AGTACT | 2 cut(s) | 1242, 2786 |
| Bme1390I | CCNGG | 4 cut(s) | 1421, 2492, 2582, 2711 |
| Bme18I | GGWCC | 1 cut(s) | 1315 |
| BmeT110I | CYCGRG | 1 cut(s) | 2630 |
| BmgBI | CACGTC | 1 cut(s) | 148 |
| BmgT120I | GGNCC | 1 cut(s) | 1315 |
| BmiI | GGNNCC | 3 cut(s) | 349, 824, 851 |
| BmrFI | CCNGG | 4 cut(s) | 1421, 2492, 2582, 2711 |
| BmsI | GCATC | 4 cut(s) | 50, 460, 2504, 2617 |
| BpiI | GAAGAC | 1 cut(s) | 2226 |
| BpmI | CTGGAG | 4 cut(s) | 50, 1403, 2603, 2752 |
| Bpu10I | CCTNAGC | 4 cut(s) | 216, 854, 1250, 1298 |
| Bpu14I | TTCGAA | 1 cut(s) | 51 |
| BpuEI | CTTGAG | 5 cut(s) | 355, 770, 1262, 2462, 2570 |
| BpuMI | CCSGG | 1 cut(s) | 2711 |
| Bsa29I | ATCGAT | 1 cut(s) | 1601 |
| BsaAI | YACGTR | 1 cut(s) | 1157 |
| BsaBI | GATNNNNATC | 3 cut(s) | 261, 267, 1517 |
| BsaHI | GRCGYC | 1 cut(s) | 1594 |
| BsaJI | CCNNGG | 2 cut(s) | 73, 606 |
| BsaWI | WCCGGW | 2 cut(s) | 678, 1344 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 135, 304, 481, 1011, 1351, 1473, 1945, 2018, 2085 |
| Bse118I | RCCGGY | 2 cut(s) | 678, 2647 |
| Bse1I | ACTGG | 4 cut(s) | 531, 1398, 1769, 2735 |
| Bse21I | CCTNAGG | 1 cut(s) | 1311 |
| Bse3DI | GCAATG | 4 cut(s) | 201, 1580, 2300, 2544 |
| Bse8I | GATNNNNATC | 3 cut(s) | 261, 267, 1517 |
| BseBI | CCWGG | 3 cut(s) | 1421, 2492, 2582 |
| BseCI | ATCGAT | 1 cut(s) | 1601 |
| BseDI | CCNNGG | 2 cut(s) | 73, 606 |
| BseJI | GATNNNNATC | 3 cut(s) | 261, 267, 1517 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 135, 304, 481, 1011, 1351, 1473, 1945, 2018, 2085 |
| BseMI | GCAATG | 4 cut(s) | 201, 1580, 2300, 2544 |
| BseNI | ACTGG | 4 cut(s) | 531, 1398, 1769, 2735 |
| BseRI | GAGGAG | 4 cut(s) | 480, 1047, 2128, 2244 |
| BseSI | GKGCMC | 2 cut(s) | 66, 2197 |
| BseXI | GCAGC | 6 cut(s) | 138, 795, 915, 937, 1710, 1920 |
| BseYI | CCCAGC | 2 cut(s) | 2327, 2798 |
| BsgI | GTGCAG | 1 cut(s) | 459 |
| BshFI | GGCC | 7 cut(s) | 128, 504, 558, 611, 977, 2073, 2124 |
| BshNI | GGYRCC | 1 cut(s) | 822 |
| BshTI | ACCGGT | 1 cut(s) | 678 |
| BshVI | ATCGAT | 1 cut(s) | 1601 |
| BsiHKAI | GWGCWC | 1 cut(s) | 66 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2630 |
| BsiSI | CCGG | 5 cut(s) | 679, 1345, 2598, 2648, 2711 |
| BslFI | GGGAC | 3 cut(s) | 1116, 1630, 2775 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 135, 304, 481, 1011, 1351, 1473, 1945, 2018, 2085 |
| BsmAI | GTCTC | 6 cut(s) | 192, 397, 702, 1330, 2349, 2433 |
| BsmFI | GGGAC | 3 cut(s) | 1116, 1630, 2775 |
| BsmI | GAATGC | 4 cut(s) | 328, 871, 1885, 2429 |
| BsnI | GGCC | 7 cut(s) | 128, 504, 558, 611, 977, 2073, 2124 |
| BsoBI | CYCGRG | 1 cut(s) | 2630 |
| Bsp119I | TTCGAA | 1 cut(s) | 51 |
| Bsp1286I | GDGCHC | 3 cut(s) | 66, 516, 2197 |
| Bsp1407I | TGTACA | 1 cut(s) | 2277 |
| BspACI | CCGC | 5 cut(s) | 298, 948, 1713, 1813, 2272 |
| BspANI | GGCC | 7 cut(s) | 128, 504, 558, 611, 977, 2073, 2124 |
| BspDI | ATCGAT | 1 cut(s) | 1601 |
| BspHI | TCATGA | 3 cut(s) | 208, 259, 265 |
| BspLI | GGNNCC | 3 cut(s) | 349, 824, 851 |
| BspMAI | CTGCAG | 4 cut(s) | 145, 156, 813, 1028 |
| BspMI | ACCTGC | 1 cut(s) | 291 |
| BspPI | GGATC | 5 cut(s) | 623, 674, 1023, 1336, 2366 |
| BspQI | GCTCTTC | 1 cut(s) | 246 |
| BspT104I | TTCGAA | 1 cut(s) | 51 |
| BspT107I | GGYRCC | 1 cut(s) | 822 |
| BspTI | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| BsrDI | GCAATG | 4 cut(s) | 201, 1580, 2300, 2544 |
| BsrFI | RCCGGY | 2 cut(s) | 678, 2647 |
| BsrGI | TGTACA | 1 cut(s) | 2277 |
| BsrI | ACTGG | 4 cut(s) | 531, 1398, 1769, 2735 |
| BssAI | RCCGGY | 2 cut(s) | 678, 2647 |
| BssECI | CCNNGG | 2 cut(s) | 73, 606 |
| BssNI | GRCGYC | 1 cut(s) | 1594 |
| BssT1I | CCWWGG | 2 cut(s) | 73, 606 |
| Bst2UI | CCWGG | 3 cut(s) | 1421, 2492, 2582 |
| Bst4CI | ACNGT | 6 cut(s) | 437, 673, 1190, 1834, 2571, 2789 |
| Bst6I | CTCTTC | 2 cut(s) | 246, 1392 |
| BstACI | GRCGYC | 1 cut(s) | 1594 |
| BstAFI | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| BstAUI | TGTACA | 1 cut(s) | 2277 |
| BstBAI | YACGTR | 1 cut(s) | 1157 |
| BstBI | TTCGAA | 1 cut(s) | 51 |
| BstC8I | GCNNGC | 6 cut(s) | 39, 660, 1571, 1811, 2031, 2099 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 133 |
| BstHHI | GCGC | 2 cut(s) | 1332, 1938 |
| BstMAI | GTCTC | 6 cut(s) | 192, 397, 702, 1330, 2349, 2433 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 34, 212, 297, 947, 1121, 1930, 2098 |
| BstNI | CCWGG | 3 cut(s) | 1421, 2492, 2582 |
| BstNSI | RCATGY | 2 cut(s) | 772, 1488 |
| BstSCI | CCNGG | 4 cut(s) | 1419, 2490, 2580, 2709 |
| BstSFI | CTRYAG | 6 cut(s) | 141, 152, 809, 1024, 2036, 2202 |
| BstSLI | GKGCMC | 2 cut(s) | 66, 2197 |
| BstV1I | GCAGC | 6 cut(s) | 138, 795, 915, 937, 1710, 1920 |
| BstV2I | GAAGAC | 1 cut(s) | 2226 |
| BstX2I | RGATCY | 2 cut(s) | 1015, 1841 |
| BstYI | RGATCY | 2 cut(s) | 1015, 1841 |
| Bsu15I | ATCGAT | 1 cut(s) | 1601 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1311 |
| BsuRI | GGCC | 7 cut(s) | 128, 504, 558, 611, 977, 2073, 2124 |
| BsuTUI | ATCGAT | 1 cut(s) | 1601 |
| BtgZI | GCGATG | 1 cut(s) | 2829 |
| BtrI | CACGTC | 1 cut(s) | 148 |
| BtsI | GCAGTG | 1 cut(s) | 881 |
| BtsIMutI | CAGTG | 5 cut(s) | 442, 881, 1391, 1647, 1677 |
| BveI | ACCTGC | 1 cut(s) | 291 |
| Cac8I | GCNNGC | 6 cut(s) | 39, 660, 1571, 1811, 2031, 2099 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2275 |
| CciI | TCATGA | 3 cut(s) | 208, 259, 265 |
| CfoI | GCGC | 2 cut(s) | 1332, 1938 |
| Cfr10I | RCCGGY | 2 cut(s) | 678, 2647 |
| Cfr13I | GGNCC | 1 cut(s) | 1315 |
| ClaI | ATCGAT | 1 cut(s) | 1601 |
| CseI | GACGC | 2 cut(s) | 1602, 2306 |
| Csp6I | GTAC | 7 cut(s) | 823, 959, 1241, 1270, 1294, 2278, 2785 |
| CspAI | ACCGGT | 1 cut(s) | 678 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 880, 915 |
| CviQI | GTAC | 7 cut(s) | 823, 959, 1241, 1270, 1294, 2278, 2785 |
| DraI | TTTAAA | 1 cut(s) | 1233 |
| EaeI | YGGCCR | 3 cut(s) | 556, 609, 2071 |
| Eam1104I | CTCTTC | 2 cut(s) | 246, 1392 |
| EarI | CTCTTC | 2 cut(s) | 246, 1392 |
| Eco130I | CCWWGG | 2 cut(s) | 73, 606 |
| Eco147I | AGGCCT | 3 cut(s) | 128, 977, 2124 |
| Eco24I | GRGCYC | 1 cut(s) | 516 |
| Eco47I | GGWCC | 1 cut(s) | 1315 |
| Eco57I | CTGAAG | 5 cut(s) | 987, 1702, 2193, 2252, 2437 |
| Eco81I | CCTNAGG | 1 cut(s) | 1311 |
| Eco88I | CYCGRG | 1 cut(s) | 2630 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 133 |
| EcoRII | CCWGG | 3 cut(s) | 1419, 2490, 2580 |
| EcoT14I | CCWWGG | 2 cut(s) | 73, 606 |
| EcoT22I | ATGCAT | 1 cut(s) | 2519 |
| EcoT38I | GRGCYC | 1 cut(s) | 516 |
| ErhI | CCWWGG | 2 cut(s) | 73, 606 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1271, 1303, 1982, 2014 |
| FaqI | GGGAC | 3 cut(s) | 1116, 1630, 2775 |
| FauI | CCCGC | 1 cut(s) | 1806 |
| FbaI | TGATCA | 3 cut(s) | 256, 262, 268 |
| FblI | GTMKAC | 2 cut(s) | 1159, 2754 |
| Fnu4HI | GCNGC | 8 cut(s) | 152, 809, 904, 951, 1714, 1724, 1934, 2273 |
| FriOI | GRGCYC | 1 cut(s) | 516 |
| Fsp4HI | GCNGC | 8 cut(s) | 152, 809, 904, 951, 1714, 1724, 1934, 2273 |
| FspBI | CTAG | 6 cut(s) | 74, 227, 1698, 1865, 1874, 2333 |
| GlaI | GCGC | 2 cut(s) | 1331, 1937 |
| GluI | GCNGC | 8 cut(s) | 152, 809, 904, 951, 1714, 1724, 1934, 2273 |
| GsaI | CCCAGC | 2 cut(s) | 2331, 2802 |
| GsuI | CTGGAG | 4 cut(s) | 50, 1403, 2603, 2752 |
| HaeIII | GGCC | 7 cut(s) | 128, 504, 558, 611, 977, 2073, 2124 |
| HapII | CCGG | 5 cut(s) | 679, 1345, 2598, 2648, 2711 |
| HgaI | GACGC | 2 cut(s) | 1602, 2306 |
| HhaI | GCGC | 2 cut(s) | 1332, 1938 |
| Hin1I | GRCGYC | 1 cut(s) | 1594 |
| Hin6I | GCGC | 2 cut(s) | 1330, 1936 |
| HinP1I | GCGC | 2 cut(s) | 1330, 1936 |
| HincII | GTYRAC | 2 cut(s) | 524, 676 |
| HindII | GTYRAC | 2 cut(s) | 524, 676 |
| HindIII | AAGCTT | 5 cut(s) | 35, 1567, 1807, 1853, 2042 |
| HpaI | GTTAAC | 1 cut(s) | 676 |
| HpaII | CCGG | 5 cut(s) | 679, 1345, 2598, 2648, 2711 |
| HphI | GGTGA | 5 cut(s) | 196, 401, 772, 1537, 1743 |
| Hpy166II | GTNNAC | 9 cut(s) | 64, 397, 524, 676, 959, 1160, 1783, 2062, 2755 |
| Hpy8I | GTNNAC | 9 cut(s) | 64, 397, 524, 676, 959, 1160, 1783, 2062, 2755 |
| Hpy99I | CGWCG | 2 cut(s) | 465, 1596 |
| HpyAV | CCTTC | 9 cut(s) | 118, 485, 962, 1011, 1467, 1987, 2524, 2604, 2732 |
| HpyCH4III | ACNGT | 6 cut(s) | 437, 673, 1190, 1834, 2571, 2789 |
| HpyCH4IV | ACGT | 4 cut(s) | 147, 222, 1156, 2290 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 34, 212, 297, 947, 1121, 1930, 2098 |
| HpySE526I | ACGT | 4 cut(s) | 147, 222, 1156, 2290 |
| Hsp92I | GRCGYC | 1 cut(s) | 1594 |
| HspAI | GCGC | 2 cut(s) | 1330, 1936 |
| KpnI | GGTACC | 1 cut(s) | 826 |
| Ksp22I | TGATCA | 3 cut(s) | 256, 262, 268 |
| KspAI | GTTAAC | 1 cut(s) | 676 |
| LguI | GCTCTTC | 1 cut(s) | 246 |
| LmnI | GCTCC | 4 cut(s) | 1929, 2089, 2328, 2583 |
| Lsp1109I | GCAGC | 6 cut(s) | 138, 795, 915, 937, 1710, 1920 |
| LweI | GCATC | 4 cut(s) | 50, 460, 2504, 2617 |
| MaeI | CTAG | 6 cut(s) | 74, 227, 1698, 1865, 1874, 2333 |
| MaeII | ACGT | 4 cut(s) | 147, 222, 1156, 2290 |
| MaeIII | GTNAC | 2 cut(s) | 1076, 1675 |
| MfeI | CAATTG | 1 cut(s) | 1116 |
| MflI | RGATCY | 2 cut(s) | 1015, 1841 |
| MhlI | GDGCHC | 3 cut(s) | 66, 516, 2197 |
| MlsI | TGGCCA | 1 cut(s) | 611 |
| MluNI | TGGCCA | 1 cut(s) | 611 |
| MlyI | GAGTC | 1 cut(s) | 384 |
| MmeI | TCCRAC | 6 cut(s) | 93, 375, 798, 2413, 2416, 2813 |
| Mox20I | TGGCCA | 1 cut(s) | 611 |
| Mph1103I | ATGCAT | 1 cut(s) | 2519 |
| MroXI | GAANNNNTTC | 4 cut(s) | 647, 1833, 1980, 2421 |
| MscI | TGGCCA | 1 cut(s) | 611 |
| MslI | CAYNNNNRTG | 5 cut(s) | 258, 264, 270, 276, 2522 |
| Msp20I | TGGCCA | 1 cut(s) | 611 |
| MspA1I | CMGCKG | 2 cut(s) | 950, 2272 |
| MspCI | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| MspI | CCGG | 5 cut(s) | 679, 1345, 2598, 2648, 2711 |
| MspR9I | CCNGG | 4 cut(s) | 1421, 2492, 2582, 2711 |
| MunI | CAATTG | 1 cut(s) | 1116 |
| Mva1269I | GAATGC | 4 cut(s) | 328, 871, 1885, 2429 |
| MvaI | CCWGG | 3 cut(s) | 1421, 2492, 2582 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 34, 212, 297, 947, 1121, 1930, 2098 |
| NciI | CCSGG | 1 cut(s) | 2711 |
| NlaIV | GGNNCC | 3 cut(s) | 349, 824, 851 |
| NmuCI | GTSAC | 1 cut(s) | 1675 |
| NsiI | ATGCAT | 1 cut(s) | 2519 |
| NspI | RCATGY | 2 cut(s) | 772, 1488 |
| NspV | TTCGAA | 1 cut(s) | 51 |
| PaeR7I | CTCGAG | 1 cut(s) | 2630 |
| PagI | TCATGA | 3 cut(s) | 208, 259, 265 |
| PceI | AGGCCT | 3 cut(s) | 128, 977, 2124 |
| PciI | ACATGT | 2 cut(s) | 768, 1484 |
| PciSI | GCTCTTC | 1 cut(s) | 246 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 228 |
| PctI | GAATGC | 4 cut(s) | 328, 871, 1885, 2429 |
| PdmI | GAANNNNTTC | 4 cut(s) | 647, 1833, 1980, 2421 |
| PfeI | GAWTC | 9 cut(s) | 1007, 1365, 1491, 1861, 1967, 2185, 2314, 2482, 2668 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1945 |
| PfoI | TCCNGGA | 3 cut(s) | 1419, 2580, 2709 |
| PinAI | ACCGGT | 1 cut(s) | 678 |
| PkrI | GCNGC | 8 cut(s) | 153, 810, 905, 952, 1715, 1725, 1935, 2274 |
| PleI | GAGTC | 1 cut(s) | 383 |
| PpsI | GAGTC | 1 cut(s) | 383 |
| Ppu21I | YACGTR | 1 cut(s) | 1157 |
| PscI | ACATGT | 2 cut(s) | 768, 1484 |
| PshBI | ATTAAT | 1 cut(s) | 2420 |
| Psp6I | CCWGG | 3 cut(s) | 1419, 2490, 2580 |
| PspFI | CCCAGC | 2 cut(s) | 2327, 2798 |
| PspGI | CCWGG | 3 cut(s) | 1419, 2490, 2580 |
| PspN4I | GGNNCC | 3 cut(s) | 349, 824, 851 |
| PspPI | GGNCC | 1 cut(s) | 1315 |
| PstI | CTGCAG | 4 cut(s) | 145, 156, 813, 1028 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2275 |
| PsuI | RGATCY | 2 cut(s) | 1015, 1841 |
| RsaI | GTAC | 7 cut(s) | 824, 960, 1242, 1271, 1295, 2279, 2786 |
| RsaNI | GTAC | 7 cut(s) | 823, 959, 1241, 1270, 1294, 2278, 2785 |
| RseI | CAYNNNNRTG | 5 cut(s) | 258, 264, 270, 276, 2522 |
| SapI | GCTCTTC | 1 cut(s) | 246 |
| SatI | GCNGC | 8 cut(s) | 152, 809, 904, 951, 1714, 1724, 1934, 2273 |
| Sau96I | GGNCC | 1 cut(s) | 1315 |
| ScaI | AGTACT | 2 cut(s) | 1242, 2786 |
| SchI | GAGTC | 1 cut(s) | 384 |
| ScrFI | CCNGG | 4 cut(s) | 1421, 2492, 2582, 2711 |
| SduI | GDGCHC | 3 cut(s) | 66, 516, 2197 |
| SfaNI | GCATC | 4 cut(s) | 50, 460, 2504, 2617 |
| SfcI | CTRYAG | 6 cut(s) | 141, 152, 809, 1024, 2036, 2202 |
| Sfr274I | CTCGAG | 1 cut(s) | 2630 |
| SfuI | TTCGAA | 1 cut(s) | 51 |
| SinI | GGWCC | 1 cut(s) | 1315 |
| SlaI | CTCGAG | 1 cut(s) | 2630 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 258, 264, 270, 276, 2522 |
| SseBI | AGGCCT | 3 cut(s) | 128, 977, 2124 |
| SsiI | CCGC | 5 cut(s) | 298, 948, 1713, 1813, 2272 |
| SspMI | CTAG | 6 cut(s) | 74, 227, 1698, 1865, 1874, 2333 |
| StuI | AGGCCT | 3 cut(s) | 128, 977, 2124 |
| StyD4I | CCNGG | 4 cut(s) | 1419, 2490, 2580, 2709 |
| StyI | CCWWGG | 2 cut(s) | 73, 606 |
| TaaI | ACNGT | 6 cut(s) | 437, 673, 1190, 1834, 2571, 2789 |
| TaiI | ACGT | 4 cut(s) | 150, 225, 1159, 2293 |
| TaqI | TCGA | 8 cut(s) | 51, 463, 1326, 1489, 1591, 1601, 2172, 2631 |
| TatI | WGTACW | 4 cut(s) | 958, 1240, 2277, 2784 |
| TauI | GCSGC | 2 cut(s) | 1716, 2275 |
| TfiI | GAWTC | 9 cut(s) | 1007, 1365, 1491, 1861, 1967, 2185, 2314, 2482, 2668 |
| TscAI | CASTG | 5 cut(s) | 442, 888, 1398, 1647, 1684 |
| TseFI | GTSAC | 1 cut(s) | 1675 |
| TseI | GCWGC | 6 cut(s) | 151, 808, 903, 950, 1723, 1933 |
| Tsp45I | GTSAC | 1 cut(s) | 1675 |
| TspDTI | ATGAA | 7 cut(s) | 137, 225, 779, 1357, 1654, 1973, 2520 |
| TspGWI | ACGGA | 2 cut(s) | 2690, 2789 |
| TspRI | CASTG | 5 cut(s) | 442, 888, 1398, 1647, 1684 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1945 |
| Vha464I | CTTAAG | 4 cut(s) | 1087, 1856, 1880, 2348 |
| VneI | GTGCAC | 1 cut(s) | 62 |
| VpaK11BI | GGWCC | 1 cut(s) | 1315 |
| VspI | ATTAAT | 1 cut(s) | 2420 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 133 |
| XapI | RAATTY | 4 cut(s) | 282, 689, 1071, 1763 |
| XbaI | TCTAGA | 3 cut(s) | 226, 1864, 1873 |
| XceI | RCATGY | 2 cut(s) | 772, 1488 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 523 |
| XhoI | CTCGAG | 1 cut(s) | 2630 |
| XmaJI | CCTAGG | 1 cut(s) | 73 |
| XmiI | GTMKAC | 2 cut(s) | 1159, 2754 |
| XmnI | GAANNNNTTC | 4 cut(s) | 647, 1833, 1980, 2421 |
| XspI | CTAG | 6 cut(s) | 74, 227, 1698, 1865, 1874, 2333 |
| ZrmI | AGTACT | 2 cut(s) | 1242, 2786 |
| Zsp2I | ATGCAT | 1 cut(s) | 2519 |