MD09G1164200.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000888
Physical Location & Seq
Reverse (-)
13460929 .. 13463706
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1164200.v1.1.491

Sequence Viewer

Length: 2778 bp
ATGGCAGAGAGTGCAGTAAAGTTTCTGCTCGACAAGGTTGCACCATTTTTTGAAAATGATCTGCAACTTTTGGGAGGGGTTAGGGAAGAAATTGTGTATCTGAGAGGGGAACTGGAGCGCATGACTGCGTTTCTGAGGGTTGCTGATGCTTTTGAAGAGAGTGATGAGGAAGTCAAAGTATGGGTGAGGCAAGTAAGAGACATTGCTCATGATAGCGAAGATGTTCTGGACGAATTTACACTTCTCCATGCACATGATCATGGGAAGGGGTTATACAGTTCCATCCATAGGTTTTCGTGTTGCATTAAGAACATGAAATCTCAATACCGAATTTCTGCTGAGTTACAAGGCATCAACTCGAGAATCAGAAAAATCTCGGAGGTTCATATGAGACTACGTCACAAGTTTAACATGGCTGAACAAGGTTCAGGCTCTTCAACTGCAGGCCAAACGTGGGAGGACCATCGCAGTGATGCTCTACTATTAGAGAAAACTGATATAGTTGGCATTGACGAGCCTATAAAGCAGGTGGTAGGGTGGCTTGTCACCGGTGGTTCTGGACGAGAAGTAGTTTCTGTGGCTGGAATGGGTGGACTAGGGAAGACAACCTTTGCGAAGCAAGTCTATGATGCAGCAGAAGTGAAGAAATATTTCAAAGTAAGAGCTTGGATCACGGTTACTCAGTCCTTCAAACTTGGGGAACTCCTCGTAGACATGATTCAACAGCTCCACAAAGCCATCCGAAGGCCAGTTCCAGAAGGAACAAACAATATGAACAACAATCAATTGAAAACAATGATCAAGGCCTTCCTGCAGAAAAGGAGGTACCTAATTGTTCTCGACAACGTGTGGGACATGTGCGGATGGGCCTCTCTGAAGTATGCCATGCCTAACAATACTTGTGGCAGTCGAGTCATACTAACTACTCGAAAAGCTGATGTAGCCTCGACAACCAGCAAAGAATCTGGAGGTAGAGTCTTTAATTTGGAGCCCTTGCCGCAGTTGGAGTCATGGGTGCTTTTATGCAAGAAGACATTCCAGGGGAACTCATGCCCTCCTTATTTAGAGGAAATCGGTAAGTCTATCTTAAGAAAGTGTGAGGGGCTGCCCCTTGCCATTGTGGCAGTCAGCGGTGTTTTGGCTACCAAAGACAAGCGCAGGATTGACGAGTGGGATATGGTTGGCCATAGCCTTGGTGCTGAAATGGAGGGCAATGACAAACTCAAGGACTTGAAAAAAGTACTTTCACTCAGCTTTAATGACTTACCTTACTATCTCAAGTCTTGCTTCTTGTACCTGAGCATCTTTCCCGAGGATCATCTAATCGAGCATATGAGACTTGTTCGGTTATGGATGGCAGAGGGTTTTATCGAAGAAAAACAAGGCAAAACACTAGAAGATGTTGCAGAGGACTACCTCAATGAACTGTTGAATAGAAGCATGATTCAAGTAGCAGAGACAACAACCGATGGAAGGGTCAAAAGCTTTCGTGTTCATGATCTTCTCCGTGAGATTATCACCTCCAAGATAAGAGATCAGAACTTTGCCACAATAGCTAAAGAGCAAAACATGCCATGGCCTGATAAAGTCCGACGCCTGTCAATGCATACCTCTTTGCAATACATACAGAAAAACATGTCCGCTTCTCAACTACGTTCTCTGTTTATGTTCGGAGTCACCGAGAATCCACTACTGCAAACAATTTTTCCTGGAGGTTTTAGGCTTCTTAACGTGTTAGATTTGCAAAGCTCACCTCTAAATGTTTTTCCAGTTGATGTTGTCAACCTTTTCTTTTTGAAGTATTTAAGCTTGAAAGGTACCAGGGTGAAAACCATTCCGAGATTTATAGGGAAGCTTCAGAACCTGGAGACTTTGGATCTGAAGCATTCTCTGGTCACTGAACTCCCAGTTGAAATTCTGAAACTTCAACATCTACGTCATCTTTTAGTATATCGTTATGAATTTATACCTAACGGATTTCTTCACTCAAAATATGGCTTTAAGGTGCTTCCAAACATAGGGGCTTTAACTTGCCTTCAAAAGCTTTGTTTCATCAAGGCAAACCAAGATGGAGGTGCCATTTTAAGGGAGCTGGGGAAACTAATTCAGTTGAGACGGTTAGGAATTGTACATATGAGGAAACAAGATGGAAAGGCTCTTTGTTCATCCATTGAAAAGCTGACCAAACTTCATGCGTTGTCCATAACTTCCGTAGAAGAGGATGAAATCATTGATCTGCAGCACATTTCTTCTCCCCCTCTCCTGCTTCAGCGCCTATACTTGCGAGGACGATTGGATACATTGCCTCACTGGATACCTTCTCTGCACAGCCTTGTCAGGTTACATCTGAAATGGAGTAGGTTAAAGGACAATCCGCTTGTATTCCTTCAGTATCTGCCCATCCTATTGCATCTCGAACTATCTCAGGTCTTTGAAGGAGACACATTGTGTTTTGGAGCTGGAGGATTTAAGAAGCTCAAACATTTAGGCATTGGTGAATTTGATGAGCTTAGATGTATAGAGGTGCAGGTGGGAGCAATGCTGTCCGTTGAAAAGCTCAGTATCACGCACTGTAAGTCGTTAGAGAAGGTGCCATCCGGGATTGAACACCTGAACAAGCTGAAGGTGCTTGAATTATTTAATATGCCGGCAAAGTTATTCAAGACACTGAGTCCACACGAACTAGGCAACGATTATTGGAAGGTTGCGCATGTCCCGGAAGTTTATTTCACCAATTTGAGAGAGTCTCGGCGGAGGTCTACCCTTTGGAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

926

Amino Acids

105.69

Weight (kDa)

9.03

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 4.7e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 350 1.2e-39 NB-ARC domain
WHD_DRP PF23559 435 - 506 5.8e-27 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 877 2.1e-48 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 517, 2518
Acc16I TGCGCA 1 cut(s) 2709
Acc36I ACCTGC 2 cut(s) 517, 2518
Acc65I GGTACC 2 cut(s) 825, 1817
AccB1I GGYRCC 4 cut(s) 825, 1817, 2075, 2590
AccI GTMKAC 2 cut(s) 711, 2759
AciI CCGC 6 cut(s) 861, 998, 1131, 1641, 2375, 2752
AclWI GGATC 3 cut(s) 677, 1323, 1884
AcoI YGGCCR 1 cut(s) 1183
AcsI RAATTY 5 cut(s) 233, 330, 1914, 1961, 2498
AcuI CTGAAG 6 cut(s) 896, 1841, 1901, 2252, 2372, 2642
AcyI GRCGYC 1 cut(s) 1594
AdeI CACNNNGTG 1 cut(s) 2448
AfaI GTAC 5 cut(s) 827, 1242, 1295, 1819, 2130
AfiI CCNNNNNNNGG 8 cut(s) 288, 454, 744, 1473, 2018, 2085, 2769, 2770
AflII CTTAAG 1 cut(s) 1087
AflIII ACRYGT 4 cut(s) 846, 855, 1635, 1731
AgeI ACCGGT 1 cut(s) 548
AhdI GACNNNNNGTC 1 cut(s) 2670
AjnI CCWGG 4 cut(s) 1038, 1708, 1820, 1863
Alw26I GTCTC 8 cut(s) 192, 385, 1330, 1451, 1862, 2107, 2433, 2751
AlwI GGATC 3 cut(s) 677, 1323, 1884
AlwNI CAGNNNCTG 2 cut(s) 1864, 2395
Ama87I CYCGRG 2 cut(s) 358, 1310
AoxI GGCC 6 cut(s) 445, 746, 804, 867, 1183, 1577
ApeKI GCWGC 3 cut(s) 632, 1105, 2239
ApoI RAATTY 5 cut(s) 233, 330, 1914, 1961, 2498
AsiGI ACCGGT 1 cut(s) 548
Asp700I GAANNNNTTC 3 cut(s) 222, 650, 1034
Asp718I GGTACC 2 cut(s) 825, 1817
AspLEI GCGC 4 cut(s) 120, 1158, 2274, 2710
AspS9I GGNCC 2 cut(s) 460, 867
AsuC2I CCSGG 2 cut(s) 2599, 2717
AsuHPI GGTGA 8 cut(s) 196, 538, 1510, 1669, 1743, 1837, 2507, 2722
AvaI CYCGRG 2 cut(s) 358, 1310
AvaII GGWCC 1 cut(s) 460
BaeI ACNNNNGTAYC 2 cut(s) 1809, 1842
BalI TGGCCA 1 cut(s) 1185
BanI GGYRCC 4 cut(s) 825, 1817, 2075, 2590
BanII GRGCYC 1 cut(s) 993
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbsI GAAGAC 2 cut(s) 608, 1037
BbvI GCAGC 3 cut(s) 644, 1092, 2251
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 4 cut(s) 1040, 1710, 1822, 1865
BciVI GTATCC 2 cut(s) 2290, 2307
BclI TGATCA 2 cut(s) 256, 798
BcnI CCSGG 2 cut(s) 2599, 2717
BcoDI GTCTC 8 cut(s) 192, 385, 1330, 1451, 1862, 2107, 2433, 2751
BfaI CTAG 3 cut(s) 596, 1394, 2684
BfmI CTRYAG 3 cut(s) 441, 812, 2237
BfoI RGCGCY 1 cut(s) 2275
BfrI CTTAAG 1 cut(s) 1087
BfuAI ACCTGC 2 cut(s) 517, 2518
BfuI GTATCC 2 cut(s) 2290, 2307
BglI GCCNNNNNGGC 1 cut(s) 1121
BisI GCNGC 4 cut(s) 633, 998, 1106, 2240
BlsI GCNGC 4 cut(s) 634, 999, 1107, 2241
BmcAI AGTACT 1 cut(s) 1242
Bme1390I CCNGG 6 cut(s) 1040, 1710, 1822, 1865, 2599, 2717
Bme18I GGWCC 1 cut(s) 460
BmeRI GACNNNNNGTC 1 cut(s) 2670
BmeT110I CYCGRG 2 cut(s) 358, 1310
BmgT120I GGNCC 2 cut(s) 460, 867
BmiI GGNNCC 5 cut(s) 827, 990, 1819, 2077, 2592
BmrFI CCNGG 6 cut(s) 1040, 1710, 1822, 1865, 2599, 2717
BmrI ACTGGG 1 cut(s) 1901
BmsI GCATC 6 cut(s) 136, 360, 463, 619, 1311, 2419
BmuI ACTGGG 1 cut(s) 1901
BoxI GACNNNNGTC 1 cut(s) 1597
BpiI GAAGAC 2 cut(s) 608, 1037
BplI GAGNNNNNCTC 2 cut(s) 2731, 2763
BpmI CTGGAG 5 cut(s) 134, 987, 1731, 1886, 2481
Bpu10I CCTNAGC 1 cut(s) 1298
BpuEI CTTGAG 2 cut(s) 1208, 1262
BpuMI CCSGG 2 cut(s) 2599, 2717
BsaHI GRCGYC 1 cut(s) 1594
BsaJI CCNNGG 5 cut(s) 1039, 1192, 1311, 1574, 1821
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 8 cut(s) 288, 454, 744, 1473, 2018, 2085, 2769, 2770
Bse118I RCCGGY 2 cut(s) 548, 2647
Bse1I ACTGG 5 cut(s) 117, 749, 1769, 1907, 2315
Bse3DI GCAATG 4 cut(s) 201, 1219, 2300, 2544
BseBI CCWGG 4 cut(s) 1040, 1710, 1822, 1865
BseDI CCNNGG 5 cut(s) 1039, 1192, 1311, 1574, 1821
BseGI GGATG 8 cut(s) 282, 738, 869, 1359, 2165, 2227, 2400, 2594
BseLI CCNNNNNNNGG 8 cut(s) 288, 454, 744, 1473, 2018, 2085, 2769, 2770
BseMI GCAATG 4 cut(s) 201, 1219, 2300, 2544
BseMII CTCAG 9 cut(s) 92, 125, 330, 695, 1264, 1289, 2438, 2572, 2660
BseNI ACTGG 5 cut(s) 117, 749, 1769, 1907, 2315
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 3 cut(s) 644, 1092, 2251
BseYI CCCAGC 1 cut(s) 2092
BsgI GTGCAG 3 cut(s) 33, 2309, 2546
BshFI GGCC 6 cut(s) 447, 748, 806, 869, 1185, 1579
BshNI GGYRCC 4 cut(s) 825, 1817, 2075, 2590
BshTI ACCGGT 1 cut(s) 548
BsiHKCI CYCGRG 2 cut(s) 358, 1310
BsiSI CCGG 4 cut(s) 549, 2598, 2648, 2717
BslFI GGGAC 2 cut(s) 866, 2699
BslI CCNNNNNNNGG 8 cut(s) 288, 454, 744, 1473, 2018, 2085, 2769, 2770
BsmAI GTCTC 8 cut(s) 192, 385, 1330, 1451, 1862, 2107, 2433, 2751
BsmBI CGTCTC 1 cut(s) 2107
BsmFI GGGAC 2 cut(s) 866, 2699
BsmI GAATGC 1 cut(s) 1885
BsnI GGCC 6 cut(s) 447, 748, 806, 869, 1185, 1579
BsoBI CYCGRG 2 cut(s) 358, 1310
Bsp1286I GDGCHC 1 cut(s) 993
Bsp1407I TGTACA 1 cut(s) 2128
Bsp143I GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
Bsp19I CCATGG 1 cut(s) 1574
BspACI CCGC 6 cut(s) 861, 998, 1131, 1641, 2375, 2752
BspANI GGCC 6 cut(s) 447, 748, 806, 869, 1185, 1579
BspCNI CTCAG 9 cut(s) 93, 126, 331, 694, 1263, 1290, 2437, 2571, 2661
BspHI TCATGA 2 cut(s) 208, 1495
BspLI GGNNCC 5 cut(s) 827, 990, 1819, 2077, 2592
BspMAI CTGCAG 3 cut(s) 445, 816, 2241
BspMI ACCTGC 2 cut(s) 517, 2518
BspPI GGATC 3 cut(s) 677, 1323, 1884
BspQI GCTCTTC 1 cut(s) 439
BspT107I GGYRCC 4 cut(s) 825, 1817, 2075, 2590
BspTI CTTAAG 1 cut(s) 1087
BsrDI GCAATG 4 cut(s) 201, 1219, 2300, 2544
BsrFI RCCGGY 2 cut(s) 548, 2647
BsrGI TGTACA 1 cut(s) 2128
BsrI ACTGG 5 cut(s) 117, 749, 1769, 1907, 2315
BssAI RCCGGY 2 cut(s) 548, 2647
BssECI CCNNGG 5 cut(s) 1039, 1192, 1311, 1574, 1821
BssMI GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
BssNI GRCGYC 1 cut(s) 1594
BssT1I CCWWGG 2 cut(s) 1192, 1574
Bst2UI CCWGG 4 cut(s) 1040, 1710, 1822, 1865
Bst4CI ACNGT 5 cut(s) 278, 676, 1428, 2118, 2573
Bst6I CTCTTC 3 cut(s) 150, 439, 2211
BstACI GRCGYC 1 cut(s) 1594
BstAFI CTTAAG 1 cut(s) 1087
BstAPI GCANNNNNTGC 2 cut(s) 11, 1570
BstAUI TGTACA 1 cut(s) 2128
BstC8I GCNNGC 2 cut(s) 445, 2649
BstDSI CCRYGG 1 cut(s) 1574
BstF5I GGATG 8 cut(s) 282, 738, 869, 1359, 2165, 2227, 2400, 2594
BstH2I RGCGCY 1 cut(s) 2275
BstHHI GCGC 4 cut(s) 120, 1158, 2274, 2710
BstKTI GATC 9 cut(s) 61, 259, 672, 801, 1318, 1501, 1537, 1879, 2236
BstMAI GTCTC 8 cut(s) 192, 385, 1330, 1451, 1862, 2107, 2433, 2751
BstMBI GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
BstMWI GCNNNNNNNGC 8 cut(s) 11, 523, 941, 997, 1121, 1553, 1570, 2626
BstNI CCWGG 4 cut(s) 1040, 1710, 1822, 1865
BstNSI RCATGY 4 cut(s) 859, 1573, 1639, 2714
BstPAI GACNNNNGTC 1 cut(s) 1597
BstSCI CCNGG 6 cut(s) 1038, 1708, 1820, 1863, 2597, 2715
BstSFI CTRYAG 3 cut(s) 441, 812, 2237
BstV1I GCAGC 3 cut(s) 644, 1092, 2251
BstV2I GAAGAC 2 cut(s) 608, 1037
BstX2I RGATCY 1 cut(s) 1876
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 1 cut(s) 1876
BsuI GTATCC 2 cut(s) 2290, 2307
BsuRI GGCC 6 cut(s) 447, 748, 806, 869, 1185, 1579
BtgI CCRYGG 1 cut(s) 1574
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 8 cut(s) 282, 738, 869, 1359, 2165, 2227, 2400, 2594
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 5 cut(s) 475, 1896, 2308, 2569, 2666
BveI ACCTGC 2 cut(s) 517, 2518
Cac8I GCNNGC 2 cut(s) 445, 2649
CaiI CAGNNNCTG 2 cut(s) 1864, 2395
CciI TCATGA 2 cut(s) 208, 1495
CfoI GCGC 4 cut(s) 120, 1158, 2274, 2710
Cfr10I RCCGGY 2 cut(s) 548, 2647
Cfr13I GGNCC 2 cut(s) 460, 867
CseI GACGC 1 cut(s) 1602
Csp6I GTAC 5 cut(s) 826, 1241, 1294, 1818, 2129
CspAI ACCGGT 1 cut(s) 548
CspCI CAANNNNNGTGG 2 cut(s) 883, 918
CviQI GTAC 5 cut(s) 826, 1241, 1294, 1818, 2129
DpnI GATC 9 cut(s) 60, 258, 671, 800, 1317, 1500, 1536, 1878, 2235
DpnII GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
DraIII CACNNNGTG 1 cut(s) 2448
DriI GACNNNNNGTC 1 cut(s) 2670
EaeI YGGCCR 1 cut(s) 1183
Eam1104I CTCTTC 3 cut(s) 150, 439, 2211
Eam1105I GACNNNNNGTC 1 cut(s) 2670
EarI CTCTTC 3 cut(s) 150, 439, 2211
EciI GGCGGA 1 cut(s) 2767
Eco130I CCWWGG 2 cut(s) 1192, 1574
Eco147I AGGCCT 1 cut(s) 806
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 6 cut(s) 896, 1841, 1901, 2252, 2372, 2642
Eco88I CYCGRG 2 cut(s) 358, 1310
EcoRII CCWGG 4 cut(s) 1038, 1708, 1820, 1863
EcoT14I CCWWGG 2 cut(s) 1192, 1574
EcoT22I ATGCAT 1 cut(s) 1608
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 2 cut(s) 1192, 1574
Esp3I CGTCTC 1 cut(s) 2107
FalI AAGNNNNNCTT 6 cut(s) 593, 625, 1070, 1102, 1271, 1303
FaqI GGGAC 2 cut(s) 866, 2699
FauNDI CATATG 3 cut(s) 387, 1332, 2133
FbaI TGATCA 2 cut(s) 256, 798
FblI GTMKAC 2 cut(s) 711, 2759
Fnu4HI GCNGC 4 cut(s) 633, 998, 1106, 2240
FokI GGATG 8 cut(s) 269, 725, 876, 1366, 2152, 2234, 2387, 2581
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 4 cut(s) 633, 998, 1106, 2240
FspBI CTAG 3 cut(s) 596, 1394, 2684
FspI TGCGCA 1 cut(s) 2709
GlaI GCGC 4 cut(s) 119, 1157, 2273, 2709
GluI GCNGC 4 cut(s) 633, 998, 1106, 2240
GsaI CCCAGC 1 cut(s) 2096
GsuI CTGGAG 5 cut(s) 134, 987, 1731, 1886, 2481
HaeII RGCGCY 1 cut(s) 2275
HaeIII GGCC 6 cut(s) 447, 748, 806, 869, 1185, 1579
HapII CCGG 4 cut(s) 549, 2598, 2648, 2717
HgaI GACGC 1 cut(s) 1602
HhaI GCGC 4 cut(s) 120, 1158, 2274, 2710
Hin1I GRCGYC 1 cut(s) 1594
Hin6I GCGC 4 cut(s) 118, 1156, 2272, 2708
HinP1I GCGC 4 cut(s) 118, 1156, 2272, 2708
HincII GTYRAC 1 cut(s) 1783
HindII GTYRAC 1 cut(s) 1783
HindIII AAGCTT 4 cut(s) 1483, 1807, 1853, 2042
HpaII CCGG 4 cut(s) 549, 2598, 2648, 2717
HphI GGTGA 8 cut(s) 196, 538, 1510, 1669, 1743, 1837, 2507, 2722
Hpy166II GTNNAC 5 cut(s) 593, 712, 1783, 2675, 2760
Hpy8I GTNNAC 5 cut(s) 593, 712, 1783, 2675, 2760
Hpy99I CGWCG 1 cut(s) 1596
HpyCH4III ACNGT 5 cut(s) 278, 676, 1428, 2118, 2573
HpyCH4IV ACGT 6 cut(s) 397, 452, 846, 1654, 1731, 1936
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 523, 941, 997, 1121, 1553, 1570, 2626
HpySE526I ACGT 6 cut(s) 397, 452, 846, 1654, 1731, 1936
Hsp92I GRCGYC 1 cut(s) 1594
HspAI GCGC 4 cut(s) 118, 1156, 2272, 2708
KpnI GGTACC 2 cut(s) 829, 1821
KroI GCCGGC 1 cut(s) 2647
KroNI GCCGGC 1 cut(s) 2649
Ksp22I TGATCA 2 cut(s) 256, 798
Kzo9I GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 6 cut(s) 115, 732, 988, 2089, 2456, 2534
Lsp1109I GCAGC 3 cut(s) 644, 1092, 2251
LweI GCATC 6 cut(s) 136, 360, 463, 619, 1311, 2419
MaeI CTAG 3 cut(s) 596, 1394, 2684
MaeII ACGT 6 cut(s) 397, 452, 846, 1654, 1731, 1936
MaeIII GTNAC 7 cut(s) 342, 398, 544, 676, 1675, 1894, 2340
MalI GATC 9 cut(s) 60, 258, 671, 800, 1317, 1500, 1536, 1878, 2235
MboI GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
MfeI CAATTG 1 cut(s) 785
MflI RGATCY 1 cut(s) 1876
MhlI GDGCHC 1 cut(s) 993
MlsI TGGCCA 1 cut(s) 1185
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 6 cut(s) 921, 984, 1016, 1683, 2680, 2753
MmeI TCCRAC 2 cut(s) 984, 1615
Mox20I TGGCCA 1 cut(s) 1185
Mph1103I ATGCAT 1 cut(s) 1608
MroNI GCCGGC 1 cut(s) 2647
MroXI GAANNNNTTC 3 cut(s) 222, 650, 1034
MscI TGGCCA 1 cut(s) 1185
MslI CAYNNNNRTG 3 cut(s) 252, 258, 468
Msp20I TGGCCA 1 cut(s) 1185
MspA1I CMGCKG 1 cut(s) 1131
MspCI CTTAAG 1 cut(s) 1087
MspI CCGG 4 cut(s) 549, 2598, 2648, 2717
MspR9I CCNGG 6 cut(s) 1040, 1710, 1822, 1865, 2599, 2717
MunI CAATTG 1 cut(s) 785
Mva1269I GAATGC 1 cut(s) 1885
MvaI CCWGG 4 cut(s) 1040, 1710, 1822, 1865
MwoI GCNNNNNNNGC 8 cut(s) 11, 523, 941, 997, 1121, 1553, 1570, 2626
NaeI GCCGGC 1 cut(s) 2649
NciI CCSGG 2 cut(s) 2599, 2717
NcoI CCATGG 1 cut(s) 1574
NdeI CATATG 3 cut(s) 387, 1332, 2133
NdeII GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
NgoMIV GCCGGC 1 cut(s) 2647
NlaIV GGNNCC 5 cut(s) 827, 990, 1819, 2077, 2592
NmeAIII GCCGAG 1 cut(s) 2728
NmuCI GTSAC 4 cut(s) 398, 544, 1675, 1894
NsbI TGCGCA 1 cut(s) 2709
NsiI ATGCAT 1 cut(s) 1608
NspI RCATGY 4 cut(s) 859, 1573, 1639, 2714
PaeR7I CTCGAG 1 cut(s) 358
PagI TCATGA 2 cut(s) 208, 1495
PaqCI CACCTGC 2 cut(s) 517, 2518
PceI AGGCCT 1 cut(s) 806
PciI ACATGT 2 cut(s) 855, 1635
PciSI GCTCTTC 1 cut(s) 439
PcsI WCGNNNNNNNCGW 1 cut(s) 1677
PctI GAATGC 1 cut(s) 1885
PdiI GCCGGC 1 cut(s) 2649
PdmI GAANNNNTTC 3 cut(s) 222, 650, 1034
PfeI GAWTC 5 cut(s) 363, 718, 962, 1444, 1684
PflFI GACNNNGTC 1 cut(s) 396
PfoI TCCNGGA 3 cut(s) 1708, 2597, 2715
PinAI ACCGGT 1 cut(s) 548
PkrI GCNGC 4 cut(s) 634, 999, 1107, 2241
PleI GAGTC 6 cut(s) 920, 983, 1015, 1682, 2679, 2752
PpsI GAGTC 6 cut(s) 920, 983, 1015, 1682, 2679, 2752
PscI ACATGT 2 cut(s) 855, 1635
PshAI GACNNNNGTC 1 cut(s) 1597
Psp6I CCWGG 4 cut(s) 1038, 1708, 1820, 1863
PspFI CCCAGC 1 cut(s) 2092
PspGI CCWGG 4 cut(s) 1038, 1708, 1820, 1863
PspN4I GGNNCC 5 cut(s) 827, 990, 1819, 2077, 2592
PspPI GGNCC 2 cut(s) 460, 867
PstI CTGCAG 3 cut(s) 445, 816, 2241
PstNI CAGNNNCTG 2 cut(s) 1864, 2395
PsuI RGATCY 1 cut(s) 1876
PsyI GACNNNGTC 1 cut(s) 396
RsaI GTAC 5 cut(s) 827, 1242, 1295, 1819, 2130
RsaNI GTAC 5 cut(s) 826, 1241, 1294, 1818, 2129
RseI CAYNNNNRTG 3 cut(s) 252, 258, 468
SapI GCTCTTC 1 cut(s) 439
SatI GCNGC 4 cut(s) 633, 998, 1106, 2240
Sau3AI GATC 9 cut(s) 58, 256, 669, 798, 1315, 1498, 1534, 1876, 2233
Sau96I GGNCC 2 cut(s) 460, 867
ScaI AGTACT 1 cut(s) 1242
SchI GAGTC 6 cut(s) 921, 984, 1016, 1683, 2680, 2753
ScrFI CCNGG 6 cut(s) 1040, 1710, 1822, 1865, 2599, 2717
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 6 cut(s) 136, 360, 463, 619, 1311, 2419
SfcI CTRYAG 3 cut(s) 441, 812, 2237
Sfr274I CTCGAG 1 cut(s) 358
SgrAI CRCCGGYG 1 cut(s) 548
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 1 cut(s) 358
SmiMI CAYNNNNRTG 3 cut(s) 252, 258, 468
SmlI CTYRAG 4 cut(s) 358, 1087, 1223, 1277
SmoI CTYRAG 4 cut(s) 358, 1087, 1223, 1277
SseBI AGGCCT 1 cut(s) 806
SsiI CCGC 6 cut(s) 861, 998, 1131, 1641, 2375, 2752
SspI AATATT 1 cut(s) 650
SspMI CTAG 3 cut(s) 596, 1394, 2684
StuI AGGCCT 1 cut(s) 806
StyD4I CCNGG 6 cut(s) 1038, 1708, 1820, 1863, 2597, 2715
StyI CCWWGG 2 cut(s) 1192, 1574
TaaI ACNGT 5 cut(s) 278, 676, 1428, 2118, 2573
TaiI ACGT 6 cut(s) 400, 455, 849, 1657, 1734, 1939
TaqI TCGA 9 cut(s) 30, 359, 840, 910, 928, 947, 1326, 1371, 2415
TatI WGTACW 2 cut(s) 1240, 2128
TauI GCSGC 1 cut(s) 1000
TfiI GAWTC 5 cut(s) 363, 718, 962, 1444, 1684
TscAI CASTG 5 cut(s) 475, 1903, 2315, 2576, 2673
TseFI GTSAC 4 cut(s) 398, 544, 1675, 1894
TseI GCWGC 3 cut(s) 632, 1105, 2239
Tsp45I GTSAC 4 cut(s) 398, 544, 1675, 1894
TspGWI ACGGA 4 cut(s) 1496, 1989, 2200, 2536
TspRI CASTG 5 cut(s) 475, 1903, 2315, 2576, 2673
Tth111I GACNNNGTC 1 cut(s) 396
Vha464I CTTAAG 1 cut(s) 1087
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 5 cut(s) 233, 330, 1914, 1961, 2498
XceI RCATGY 4 cut(s) 859, 1573, 1639, 2714
XhoI CTCGAG 1 cut(s) 358
XmiI GTMKAC 2 cut(s) 711, 2759
XmnI GAANNNNTTC 3 cut(s) 222, 650, 1034
XspI CTAG 3 cut(s) 596, 1394, 2684
ZrmI AGTACT 1 cut(s) 1242
Zsp2I ATGCAT 1 cut(s) 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.