Rmu_sc0007576.1_g000012

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007576.1
OG0000888
Physical Location & Seq
Reverse (-)
50622 .. 52253
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007576.1_g000012.1.cds

Sequence Viewer

Length: 1632 bp
atggcggagagtgcagtcaagtttctgcttgacaagcttgcacccctttttgaaaatgatttgcaacttttgggaggggttagggaagaaattgtgtatctaagaggagagctagagcggatgacggcttttctgagggttgctgatgcatctgaagagagtgatgaggaactcaaagtatgggttaagcaactaagagacattgctcatgacagtgaagatgttcttgatgaatttacacttctgcaggcgcatgatcatggggagggtttatatggttcaatccataggctttcttgctgcattaagaacacaaaagcacgataccgaattgcttctgaattacaaggcatcaacttgagaatcagaaaaatctcggaggttcacaagagactccgtcacaaattcaatatggctgaacaaggtgcaggctccagcagtgcatgcaatacatgggatgaccatcgaggtgatgctctacttctagagaagactgatattgtaggcattgacaagcccataaagcagctggttggctggctagtccagggtggttctggacgtgaagtagtttctgtggctggaatgggggggatggggaaaactaccctggtcaagcaagtatatgatgctgcagaagtgaagaaacatttcaaagtttgtgcttggatcactgttactcggtctttcaaacttggagaactcctcaaagacatggttcatcaactccacaacgcaatccgcaggcctgttccagaaggaatcaataccatgaacaacaatcacttgaaaacaataatcaaggacttcctgcagaaaaggaggtatctcattgttttagatgatgtatggcacttgcatggatgggatgctgtgaaatatgcattgcctaacaatatttctggtagccgagtcattctcactactcgaaatgctgaagtagcctctacaatcagtgtacaatctggagggaaagcctacaatttggagcccttgccccaatcggagtcttgggaacttttatgcaagaagacattccaggggaaatcatgccctcctcatttagaggaaatctgtactgatgttttaaagaaatgtgaggggatgccgcttgcaattgtggcggtcagtggtgttttgtctaccaaggacaagcgtcggattgatgagtgggatatggttggccatagtcttggagctgaaattgagggcaatgacaaactgagggacttaaaaaaagtactctctctcagtttcaatgatttaccgtactatctaaaatcttgcttcttgtacctgagcatctttcctgaggattatctaattgagcatatgaggcttgttcgattatggatggcagaggggttcgtcgaaccaaaagatggcaaaacattggaagatgttgcagaggactacctcagtgaactattgaatagaggcatgattcaagctgctgacacaacatctgatggaagggtcaagaacattcgtgttcatgacctttttcgagagatcatcacctctaagataagagatcagaactttgccacaatcattatggatggcagaggggttcgtcgaaccaaaagatggcaaaacattggaagatgttgcagaggactacctcagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

543

Amino Acids

61.58

Weight (kDa)

7.93

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1382, 1590
AccBSI CCGCTC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 1142
AciI CCGC 5 cut(s) 5, 118, 742, 1109, 1124
AclWI GGATC 1 cut(s) 677
AcoI YGGCCR 1 cut(s) 1183
AcsI RAATTY 2 cut(s) 233, 404
AcuI CTGAAG 2 cut(s) 174, 957
AfaI GTAC 5 cut(s) 960, 1078, 1242, 1271, 1295
AfiI CCNNNNNNNGG 2 cut(s) 1382, 1590
AgsI TTSAA 9 cut(s) 53, 282, 409, 655, 691, 790, 1258, 1432, 1448
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 3 cut(s) 546, 609, 1038
AluBI AGCT 5 cut(s) 37, 112, 529, 1199, 1451
AluI AGCT 5 cut(s) 37, 112, 529, 1199, 1451
Alw26I GTCTC 2 cut(s) 192, 385
AlwI GGATC 1 cut(s) 677
AoxI GGCC 2 cut(s) 746, 1183
ApeKI GCWGC 4 cut(s) 300, 526, 632, 1451
ApoI RAATTY 2 cut(s) 233, 404
Asp700I GAANNNNTTC 4 cut(s) 222, 334, 650, 1034
AspLEI GCGC 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 482, 1510
AxyI CCTNAGG 1 cut(s) 1311
BalI TGGCCA 1 cut(s) 1185
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 2 cut(s) 497, 1037
BbvI GCAGC 4 cut(s) 287, 538, 619, 1438
BccI CCATC 8 cut(s) 471, 589, 858, 1348, 1376, 1463, 1556, 1584
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 3 cut(s) 548, 611, 1040
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 192, 385
BfaI CTAG 3 cut(s) 113, 485, 542
BfmI CTRYAG 3 cut(s) 245, 633, 812
BisI GCNGC 5 cut(s) 301, 527, 633, 1109, 1452
BlsI GCNGC 5 cut(s) 302, 528, 634, 1110, 1453
BmcAI AGTACT 1 cut(s) 1242
Bme1390I CCNGG 3 cut(s) 548, 611, 1040
BmgBI CACGTC 1 cut(s) 563
BmiI GGNNCC 2 cut(s) 433, 990
BmrFI CCNGG 3 cut(s) 548, 611, 1040
BmsI GCATC 8 cut(s) 136, 158, 360, 463, 619, 859, 1095, 1311
BoxI GACNNNNGTC 1 cut(s) 1155
BpiI GAAGAC 2 cut(s) 497, 1037
BplI GAGNNNNNCTC 4 cut(s) 690, 722, 903, 935
BpmI CTGGAG 2 cut(s) 418, 987
Bpu10I CCTNAGC 1 cut(s) 1298
BpuEI CTTGAG 1 cut(s) 379
BsaBI GATNNNNATC 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 547, 609, 1039, 1146
Bsc4I CCNNNNNNNGG 2 cut(s) 1382, 1590
Bse21I CCTNAGG 1 cut(s) 1311
Bse3DI GCAATG 3 cut(s) 201, 884, 1219
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 3 cut(s) 548, 611, 1040
BseDI CCNNGG 4 cut(s) 547, 609, 1039, 1146
BseGI GGATG 8 cut(s) 126, 463, 600, 869, 874, 1110, 1359, 1567
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 1382, 1590
BseMI GCAATG 3 cut(s) 201, 884, 1219
BseMII CTCAG 6 cut(s) 125, 1214, 1264, 1289, 1302, 1432
BseRI GAGGAG 3 cut(s) 120, 695, 1047
BseXI GCAGC 4 cut(s) 287, 538, 619, 1438
BsgI GTGCAG 2 cut(s) 33, 447
BshFI GGCC 2 cut(s) 748, 1185
BslFI GGGAC 1 cut(s) 1241
BslI CCNNNNNNNGG 2 cut(s) 1382, 1590
BsmAI GTCTC 2 cut(s) 192, 385
BsmFI GGGAC 1 cut(s) 1241
BsnI GGCC 2 cut(s) 748, 1185
Bsp1286I GDGCHC 1 cut(s) 993
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 4 cut(s) 256, 669, 1512, 1534
BspACI CCGC 5 cut(s) 5, 118, 742, 1109, 1124
BspANI GGCC 2 cut(s) 748, 1185
BspCNI CTCAG 6 cut(s) 126, 1215, 1263, 1290, 1303, 1431
BspHI TCATGA 2 cut(s) 208, 1495
BspLI GGNNCC 2 cut(s) 433, 990
BspMAI CTGCAG 3 cut(s) 249, 637, 816
BspPI GGATC 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 3 cut(s) 201, 884, 1219
BsrGI TGTACA 1 cut(s) 958
BssECI CCNNGG 4 cut(s) 547, 609, 1039, 1146
BssMI GATC 4 cut(s) 256, 669, 1512, 1534
BssT1I CCWWGG 1 cut(s) 1146
Bst2UI CCWGG 3 cut(s) 548, 611, 1040
Bst4CI ACNGT 3 cut(s) 215, 676, 1269
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 444
BstAUI TGTACA 1 cut(s) 958
BstC8I GCNNGC 7 cut(s) 39, 249, 430, 445, 539, 746, 1113
BstF5I GGATG 8 cut(s) 126, 463, 600, 869, 874, 1110, 1359, 1567
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 4 cut(s) 259, 672, 1515, 1537
BstMAI GTCTC 2 cut(s) 192, 385
BstMBI GATC 4 cut(s) 256, 669, 1512, 1534
BstMWI GCNNNNNNNGC 7 cut(s) 11, 34, 444, 523, 941, 1121, 1336
BstNI CCWGG 3 cut(s) 548, 611, 1040
BstNSI RCATGY 1 cut(s) 447
BstPAI GACNNNNGTC 1 cut(s) 1155
BstSCI CCNGG 3 cut(s) 546, 609, 1038
BstSFI CTRYAG 3 cut(s) 245, 633, 812
BstV1I GCAGC 4 cut(s) 287, 538, 619, 1438
BstV2I GAAGAC 2 cut(s) 497, 1037
BstXI CCANNNNNNTGG 1 cut(s) 1193
Bsu36I CCTNAGG 1 cut(s) 1311
BsuRI GGCC 2 cut(s) 748, 1185
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 8 cut(s) 126, 463, 600, 869, 874, 1110, 1359, 1567
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 6 cut(s) 220, 445, 672, 961, 1135, 1426
Cac8I GCNNGC 7 cut(s) 39, 249, 430, 445, 539, 746, 1113
CciI TCATGA 2 cut(s) 208, 1495
CfoI GCGC 1 cut(s) 253
CseI GACGC 1 cut(s) 1145
Csp6I GTAC 5 cut(s) 959, 1077, 1241, 1270, 1294
CviQI GTAC 5 cut(s) 959, 1077, 1241, 1270, 1294
DpnI GATC 4 cut(s) 258, 671, 1514, 1536
DpnII GATC 4 cut(s) 256, 669, 1512, 1534
DraI TTTAAA 1 cut(s) 1089
EaeI YGGCCR 1 cut(s) 1183
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 1146
Eco147I AGGCCT 1 cut(s) 748
Eco24I GRGCYC 1 cut(s) 993
Eco57I CTGAAG 2 cut(s) 174, 957
Eco81I CCTNAGG 1 cut(s) 1311
EcoRII CCWGG 3 cut(s) 546, 609, 1038
EcoT14I CCWWGG 1 cut(s) 1146
EcoT22I ATGCAT 2 cut(s) 151, 886
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 1146
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 1241
FauNDI CATATG 1 cut(s) 1332
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 1142
Fnu4HI GCNGC 5 cut(s) 301, 527, 633, 1109, 1452
FokI GGATG 8 cut(s) 133, 470, 607, 876, 881, 1117, 1366, 1574
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 5 cut(s) 301, 527, 633, 1109, 1452
FspBI CTAG 3 cut(s) 113, 485, 542
GlaI GCGC 1 cut(s) 252
GluI GCNGC 5 cut(s) 301, 527, 633, 1109, 1452
GsuI CTGGAG 2 cut(s) 418, 987
HaeIII GGCC 2 cut(s) 748, 1185
HgaI GACGC 1 cut(s) 1145
HhaI GCGC 1 cut(s) 253
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 6 cut(s) 363, 393, 762, 912, 1007, 1444
HphI GGTGA 2 cut(s) 482, 1510
Hpy166II GTNNAC 4 cut(s) 385, 959, 1143, 1424
Hpy188I TCNGA 9 cut(s) 135, 154, 340, 368, 379, 1006, 1161, 1468, 1539
Hpy8I GTNNAC 4 cut(s) 385, 959, 1143, 1424
Hpy99I CGWCG 3 cut(s) 1161, 1373, 1581
HpyAV CCTTC 2 cut(s) 752, 1467
HpyCH4III ACNGT 3 cut(s) 215, 676, 1269
HpyCH4IV ACGT 1 cut(s) 562
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 34, 444, 523, 941, 1121, 1336
HpySE526I ACGT 1 cut(s) 562
HspAI GCGC 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 256, 669, 1512, 1534
LmnI GCTCC 3 cut(s) 437, 988, 1196
Lsp1109I GCAGC 4 cut(s) 287, 538, 619, 1438
LweI GCATC 8 cut(s) 136, 158, 360, 463, 619, 859, 1095, 1311
MaeI CTAG 3 cut(s) 113, 485, 542
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 2 cut(s) 398, 676
MalI GATC 4 cut(s) 258, 671, 1514, 1536
MbiI CCGCTC 1 cut(s) 118
MboI GATC 4 cut(s) 256, 669, 1512, 1534
MboII GAAGA 8 cut(s) 98, 167, 230, 502, 655, 1042, 1409, 1617
MfeI CAATTG 1 cut(s) 1116
MhlI GDGCHC 1 cut(s) 993
MlsI TGGCCA 1 cut(s) 1185
MluCI AATT 9 cut(s) 90, 233, 330, 341, 404, 982, 1116, 1203, 1323
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 3 cut(s) 387, 921, 1016
MmeI TCCRAC 1 cut(s) 1139
Mox20I TGGCCA 1 cut(s) 1185
Mph1103I ATGCAT 2 cut(s) 151, 886
MroXI GAANNNNTTC 4 cut(s) 222, 334, 650, 1034
MscI TGGCCA 1 cut(s) 1185
MseI TTAA 4 cut(s) 186, 306, 1088, 1232
MslI CAYNNNNRTG 4 cut(s) 213, 258, 468, 858
Msp20I TGGCCA 1 cut(s) 1185
MspA1I CMGCKG 1 cut(s) 529
MspR9I CCNGG 3 cut(s) 548, 611, 1040
MunI CAATTG 1 cut(s) 1116
MvaI CCWGG 3 cut(s) 548, 611, 1040
MwoI GCNNNNNNNGC 7 cut(s) 11, 34, 444, 523, 941, 1121, 1336
NdeI CATATG 1 cut(s) 1332
NdeII GATC 4 cut(s) 256, 669, 1512, 1534
NlaIV GGNNCC 2 cut(s) 433, 990
NmeAIII GCCGAG 1 cut(s) 935
NmuCI GTSAC 1 cut(s) 398
NsiI ATGCAT 2 cut(s) 151, 886
NspI RCATGY 1 cut(s) 447
PaeI GCATGC 1 cut(s) 447
PagI TCATGA 2 cut(s) 208, 1495
PceI AGGCCT 1 cut(s) 748
PdmI GAANNNNTTC 4 cut(s) 222, 334, 650, 1034
PfeI GAWTC 3 cut(s) 363, 762, 1444
PflFI GACNNNGTC 1 cut(s) 396
PflMI CCANNNNNTGG 2 cut(s) 1382, 1590
PkrI GCNGC 5 cut(s) 302, 528, 634, 1110, 1453
PleI GAGTC 3 cut(s) 387, 920, 1015
PpsI GAGTC 3 cut(s) 387, 920, 1015
PshAI GACNNNNGTC 1 cut(s) 1155
Psp6I CCWGG 3 cut(s) 546, 609, 1038
PspGI CCWGG 3 cut(s) 546, 609, 1038
PspN4I GGNNCC 2 cut(s) 433, 990
PstI CTGCAG 3 cut(s) 249, 637, 816
PsyI GACNNNGTC 1 cut(s) 396
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 5 cut(s) 960, 1078, 1242, 1271, 1295
RsaNI GTAC 5 cut(s) 959, 1077, 1241, 1270, 1294
RseI CAYNNNNRTG 4 cut(s) 213, 258, 468, 858
SaqAI TTAA 4 cut(s) 186, 306, 1088, 1232
SatI GCNGC 5 cut(s) 301, 527, 633, 1109, 1452
Sau3AI GATC 4 cut(s) 256, 669, 1512, 1534
ScaI AGTACT 1 cut(s) 1242
SchI GAGTC 3 cut(s) 387, 921, 1016
ScrFI CCNGG 3 cut(s) 548, 611, 1040
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 8 cut(s) 136, 158, 360, 463, 619, 859, 1095, 1311
SfcI CTRYAG 3 cut(s) 245, 633, 812
SmiMI CAYNNNNRTG 4 cut(s) 213, 258, 468, 858
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
SphI GCATGC 1 cut(s) 447
Sse9I AATT 9 cut(s) 90, 233, 330, 341, 404, 982, 1116, 1203, 1323
SseBI AGGCCT 1 cut(s) 748
SsiI CCGC 5 cut(s) 5, 118, 742, 1109, 1124
SspI AATATT 1 cut(s) 898
SspMI CTAG 3 cut(s) 113, 485, 542
StuI AGGCCT 1 cut(s) 748
StyD4I CCNGG 3 cut(s) 546, 609, 1038
StyI CCWWGG 1 cut(s) 1146
TaaI ACNGT 3 cut(s) 215, 676, 1269
TaiI ACGT 1 cut(s) 565
TaqI TCGA 6 cut(s) 466, 928, 1345, 1371, 1507, 1579
TaqII GACCGA 1 cut(s) 672
TasI AATT 9 cut(s) 90, 233, 330, 341, 404, 982, 1116, 1203, 1323
TatI WGTACW 3 cut(s) 958, 1076, 1240
TauI GCSGC 1 cut(s) 1111
TfiI GAWTC 3 cut(s) 363, 762, 1444
Tru1I TTAA 4 cut(s) 186, 306, 1088, 1232
Tru9I TTAA 4 cut(s) 186, 306, 1088, 1232
TscAI CASTG 6 cut(s) 220, 445, 679, 961, 1135, 1426
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 4 cut(s) 300, 526, 632, 1451
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 4 cut(s) 246, 710, 788, 1484
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 6 cut(s) 220, 445, 679, 961, 1135, 1426
Tth111I GACNNNGTC 1 cut(s) 396
Van91I CCANNNNNTGG 2 cut(s) 1382, 1590
XapI RAATTY 2 cut(s) 233, 404
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 1 cut(s) 447
XcmI CCANNNNNNNNNTGG 3 cut(s) 526, 554, 1555
XmiI GTMKAC 1 cut(s) 1142
XmnI GAANNNNTTC 4 cut(s) 222, 334, 650, 1034
XspI CTAG 3 cut(s) 113, 485, 542
ZrmI AGTACT 1 cut(s) 1242
Zsp2I ATGCAT 2 cut(s) 151, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.