Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2102 |
| AatII | GACGTC | 1 cut(s) | 1615 |
| Acc16I | TGCGCA | 1 cut(s) | 1590 |
| Acc65I | GGTACC | 1 cut(s) | 837 |
| AccB1I | GGYRCC | 2 cut(s) | 837, 864 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 70, 1963 |
| AccBSI | CCGCTC | 1 cut(s) | 2395 |
| AccIII | TCCGGA | 1 cut(s) | 753 |
| AciI | CCGC | 8 cut(s) | 574, 1198, 1519, 1716, 1731, 2131, 2290, 2393 |
| AclWI | GGATC | 8 cut(s) | 451, 638, 689, 690, 1498, 1511, 1927, 2384 |
| AcoI | YGGCCR | 2 cut(s) | 274, 1464 |
| AcsI | RAATTY | 8 cut(s) | 122, 245, 714, 943, 1605, 1979, 2516, 2642 |
| AcuI | CTGAAG | 6 cut(s) | 249, 821, 1859, 2211, 2516, 2812 |
| AcyI | GRCGYC | 1 cut(s) | 1612 |
| AfaI | GTAC | 4 cut(s) | 839, 972, 1808, 2804 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 70, 147, 1363, 1519, 1963, 2036, 2370 |
| AflII | CTTAAG | 1 cut(s) | 1898 |
| AflIII | ACRYGT | 1 cut(s) | 1496 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2688 |
| AjiI | CACGTC | 1 cut(s) | 2542 |
| AjnI | CCWGG | 3 cut(s) | 439, 568, 2508 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 605, 637, 2692, 2724 |
| AleI | CACNNNNGTG | 2 cut(s) | 870, 1565 |
| Alw21I | GWGCWC | 1 cut(s) | 2215 |
| Alw26I | GTCTC | 5 cut(s) | 397, 751, 2237, 2451, 2722 |
| Alw44I | GTGCAC | 1 cut(s) | 2211 |
| AlwI | GGATC | 8 cut(s) | 451, 638, 689, 690, 1498, 1511, 1927, 2384 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 446 |
| Aor13HI | TCCGGA | 1 cut(s) | 753 |
| AoxI | GGCC | 5 cut(s) | 274, 517, 1327, 1464, 1692 |
| ApaLI | GTGCAC | 1 cut(s) | 2211 |
| ApeKI | GCWGC | 7 cut(s) | 820, 823, 826, 918, 1200, 1645, 1741 |
| ApoI | RAATTY | 8 cut(s) | 122, 245, 714, 943, 1605, 1979, 2516, 2642 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 180, 212, 2025, 2057 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 234, 346, 1705 |
| Asp718I | GGTACC | 1 cut(s) | 837 |
| AspLEI | GCGC | 2 cut(s) | 1591, 1769 |
| AspS9I | GGNCC | 4 cut(s) | 437, 517, 1327, 1692 |
| AsuHPI | GGTGA | 5 cut(s) | 241, 1549, 1705, 1855, 2089 |
| AsuII | TTCGAA | 1 cut(s) | 51 |
| AvaII | GGWCC | 1 cut(s) | 437 |
| AxyI | CCTNAGG | 2 cut(s) | 146, 1323 |
| BaeGI | GKGCMC | 1 cut(s) | 2215 |
| BalI | TGGCCA | 1 cut(s) | 1466 |
| BamHI | GGATCC | 1 cut(s) | 1503 |
| BanI | GGYRCC | 2 cut(s) | 837, 864 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1690, 1722 |
| BbsI | GAAGAC | 3 cut(s) | 809, 1109, 2687 |
| Bbv12I | GWGCWC | 1 cut(s) | 2215 |
| BbvI | GCAGC | 7 cut(s) | 807, 810, 838, 930, 1212, 1657, 1728 |
| BceAI | ACGGC | 1 cut(s) | 289 |
| BcgI | CGANNNNNNTGC | 3 cut(s) | 28, 458, 492 |
| BciT130I | CCWGG | 3 cut(s) | 441, 570, 2510 |
| BclI | TGATCA | 3 cut(s) | 175, 268, 793 |
| BcoDI | GTCTC | 5 cut(s) | 397, 751, 2237, 2451, 2722 |
| BfaI | CTAG | 5 cut(s) | 239, 1883, 1892, 2111, 2351 |
| BfmI | CTRYAG | 2 cut(s) | 153, 824 |
| BfrI | CTTAAG | 1 cut(s) | 1898 |
| BglII | AGATCT | 1 cut(s) | 1894 |
| BlpI | GCTNAGC | 2 cut(s) | 1235, 2576 |
| BmcAI | AGTACT | 1 cut(s) | 2804 |
| Bme1390I | CCNGG | 3 cut(s) | 441, 570, 2510 |
| Bme18I | GGWCC | 1 cut(s) | 437 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2688 |
| BmgBI | CACGTC | 1 cut(s) | 2542 |
| BmgT120I | GGNCC | 4 cut(s) | 437, 517, 1327, 1692 |
| BmiI | GGNNCC | 4 cut(s) | 839, 866, 1505, 1693 |
| BmrFI | CCNGG | 3 cut(s) | 441, 570, 2510 |
| BmsI | GCATC | 6 cut(s) | 148, 299, 475, 1323, 2589, 2635 |
| BpiI | GAAGAC | 3 cut(s) | 809, 1109, 2687 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 702, 734 |
| BpmI | CTGGAG | 6 cut(s) | 729, 999, 2270, 2531, 2621, 2770 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1310, 1823 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1235, 2576 |
| Bpu14I | TTCGAA | 1 cut(s) | 51 |
| BpuEI | CTTGAG | 4 cut(s) | 355, 1849, 1922, 2669 |
| Bsa29I | ATCGAT | 1 cut(s) | 694 |
| BsaBI | GATNNNNATC | 3 cut(s) | 1425, 1529, 2854 |
| BsaHI | GRCGYC | 1 cut(s) | 1612 |
| BsaI | GGTCTC | 1 cut(s) | 2237 |
| BsaJI | CCNNGG | 4 cut(s) | 277, 569, 1204, 1839 |
| BsaWI | WCCGGW | 2 cut(s) | 753, 1356 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1459, 1489, 2125, 2155 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 70, 147, 1363, 1519, 1963, 2036, 2370 |
| Bse1I | ACTGG | 6 cut(s) | 1410, 1695, 1787, 2333, 2666, 2753 |
| Bse21I | CCTNAGG | 2 cut(s) | 146, 1323 |
| Bse3DI | GCAATG | 5 cut(s) | 161, 896, 1598, 2187, 2562 |
| Bse8I | GATNNNNATC | 3 cut(s) | 1425, 1529, 2854 |
| BseAI | TCCGGA | 1 cut(s) | 753 |
| BseBI | CCWGG | 3 cut(s) | 441, 570, 2510 |
| BseCI | ATCGAT | 1 cut(s) | 694 |
| BseDI | CCNNGG | 4 cut(s) | 277, 569, 1204, 1839 |
| BseGI | GGATG | 7 cut(s) | 138, 314, 612, 859, 2392, 2580, 2768 |
| BseJI | GATNNNNATC | 3 cut(s) | 1425, 1529, 2854 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 70, 147, 1363, 1519, 1963, 2036, 2370 |
| BseMI | GCAATG | 5 cut(s) | 161, 896, 1598, 2187, 2562 |
| BseMII | CTCAG | 8 cut(s) | 137, 381, 1249, 1276, 1301, 1314, 1814, 2189 |
| BseNI | ACTGG | 6 cut(s) | 1410, 1695, 1787, 2333, 2666, 2753 |
| BseRI | GAGGAG | 4 cut(s) | 132, 1002, 1059, 2262 |
| BseSI | GKGCMC | 1 cut(s) | 2215 |
| BseXI | GCAGC | 7 cut(s) | 807, 810, 838, 930, 1212, 1657, 1728 |
| BseYI | CCCAGC | 2 cut(s) | 1727, 2816 |
| BsgI | GTGCAG | 1 cut(s) | 1664 |
| BshFI | GGCC | 5 cut(s) | 276, 519, 1329, 1466, 1694 |
| BshNI | GGYRCC | 2 cut(s) | 837, 864 |
| BshVI | ATCGAT | 1 cut(s) | 694 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2215 |
| BsiSI | CCGG | 2 cut(s) | 754, 1357 |
| BslFI | GGGAC | 3 cut(s) | 2347, 2711, 2793 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 70, 147, 1363, 1519, 1963, 2036, 2370 |
| BsmAI | GTCTC | 5 cut(s) | 397, 751, 2237, 2451, 2722 |
| BsmBI | CGTCTC | 1 cut(s) | 751 |
| BsmFI | GGGAC | 3 cut(s) | 2347, 2711, 2793 |
| BsmI | GAATGC | 2 cut(s) | 328, 1903 |
| BsnI | GGCC | 5 cut(s) | 276, 519, 1329, 1466, 1694 |
| Bso31I | GGTCTC | 1 cut(s) | 2237 |
| Bsp119I | TTCGAA | 1 cut(s) | 51 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2215 |
| Bsp13I | TCCGGA | 1 cut(s) | 753 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1235, 2576 |
| BspACI | CCGC | 8 cut(s) | 574, 1198, 1519, 1716, 1731, 2131, 2290, 2393 |
| BspANI | GGCC | 5 cut(s) | 276, 519, 1329, 1466, 1694 |
| BspCNI | CTCAG | 8 cut(s) | 138, 382, 1248, 1275, 1302, 1315, 1815, 2190 |
| BspDI | ATCGAT | 1 cut(s) | 694 |
| BspEI | TCCGGA | 1 cut(s) | 753 |
| BspHI | TCATGA | 1 cut(s) | 220 |
| BspLI | GGNNCC | 4 cut(s) | 839, 866, 1505, 1693 |
| BspMAI | CTGCAG | 2 cut(s) | 157, 828 |
| BspPI | GGATC | 8 cut(s) | 451, 638, 689, 690, 1498, 1511, 1927, 2384 |
| BspT104I | TTCGAA | 1 cut(s) | 51 |
| BspT107I | GGYRCC | 2 cut(s) | 837, 864 |
| BspTI | CTTAAG | 1 cut(s) | 1898 |
| BspTNI | GGTCTC | 1 cut(s) | 2237 |
| BsrBI | CCGCTC | 1 cut(s) | 2395 |
| BsrDI | GCAATG | 5 cut(s) | 161, 896, 1598, 2187, 2562 |
| BsrI | ACTGG | 6 cut(s) | 1410, 1695, 1787, 2333, 2666, 2753 |
| BssECI | CCNNGG | 4 cut(s) | 277, 569, 1204, 1839 |
| BssNI | GRCGYC | 1 cut(s) | 1612 |
| BssT1I | CCWWGG | 3 cut(s) | 277, 1204, 1839 |
| Bst2UI | CCWGG | 3 cut(s) | 441, 570, 2510 |
| Bst4CI | ACNGT | 7 cut(s) | 476, 688, 911, 1852, 1998, 2589, 2807 |
| Bst6I | CTCTTC | 3 cut(s) | 746, 2346, 2831 |
| BstACI | GRCGYC | 1 cut(s) | 1612 |
| BstAFI | CTTAAG | 1 cut(s) | 1898 |
| BstBI | TTCGAA | 1 cut(s) | 51 |
| BstC8I | GCNNGC | 4 cut(s) | 39, 882, 1587, 2049 |
| BstEII | GGTNACC | 1 cut(s) | 2023 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 7 cut(s) | 138, 314, 612, 859, 2392, 2580, 2768 |
| BstHHI | GCGC | 2 cut(s) | 1591, 1769 |
| BstMAI | GTCTC | 5 cut(s) | 397, 751, 2237, 2451, 2722 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 34, 224, 826, 1133, 2116, 2290 |
| BstNI | CCWGG | 3 cut(s) | 441, 570, 2510 |
| BstNSI | RCATGY | 3 cut(s) | 884, 1500, 2726 |
| BstPI | GGTNACC | 1 cut(s) | 2023 |
| BstSCI | CCNGG | 3 cut(s) | 439, 568, 2508 |
| BstSFI | CTRYAG | 2 cut(s) | 153, 824 |
| BstSLI | GKGCMC | 1 cut(s) | 2215 |
| BstV1I | GCAGC | 7 cut(s) | 807, 810, 838, 930, 1212, 1657, 1728 |
| BstV2I | GAAGAC | 3 cut(s) | 809, 1109, 2687 |
| BstX2I | RGATCY | 4 cut(s) | 443, 1503, 1894, 1932 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1023, 2090 |
| BstYI | RGATCY | 4 cut(s) | 443, 1503, 1894, 1932 |
| Bsu15I | ATCGAT | 1 cut(s) | 694 |
| Bsu36I | CCTNAGG | 2 cut(s) | 146, 1323 |
| BsuRI | GGCC | 5 cut(s) | 276, 519, 1329, 1466, 1694 |
| BsuTUI | ATCGAT | 1 cut(s) | 694 |
| BtrI | CACGTC | 1 cut(s) | 2542 |
| BtsCI | GGATG | 7 cut(s) | 138, 314, 612, 859, 2392, 2580, 2768 |
| BtsIMutI | CAGTG | 7 cut(s) | 684, 1147, 1403, 1921, 2235, 2326, 2673 |
| Cac8I | GCNNGC | 4 cut(s) | 39, 882, 1587, 2049 |
| CaiI | CAGNNNCTG | 1 cut(s) | 446 |
| CciI | TCATGA | 1 cut(s) | 220 |
| CfoI | GCGC | 2 cut(s) | 1591, 1769 |
| Cfr13I | GGNCC | 4 cut(s) | 437, 517, 1327, 1692 |
| ClaI | ATCGAT | 1 cut(s) | 694 |
| CseI | GACGC | 2 cut(s) | 2324, 2585 |
| Csp6I | GTAC | 4 cut(s) | 838, 971, 1807, 2803 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 895, 930 |
| CviQI | GTAC | 4 cut(s) | 838, 971, 1807, 2803 |
| DraI | TTTAAA | 2 cut(s) | 1776, 2148 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2688 |
| EaeI | YGGCCR | 2 cut(s) | 274, 1464 |
| Eam1104I | CTCTTC | 3 cut(s) | 746, 2346, 2831 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2688 |
| EarI | CTCTTC | 3 cut(s) | 746, 2346, 2831 |
| EciI | GGCGGA | 1 cut(s) | 589 |
| Eco130I | CCWWGG | 3 cut(s) | 277, 1204, 1839 |
| Eco31I | GGTCTC | 1 cut(s) | 2237 |
| Eco47I | GGWCC | 1 cut(s) | 437 |
| Eco57I | CTGAAG | 6 cut(s) | 249, 821, 1859, 2211, 2516, 2812 |
| Eco81I | CCTNAGG | 2 cut(s) | 146, 1323 |
| Eco91I | GGTNACC | 1 cut(s) | 2023 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 145 |
| EcoO65I | GGTNACC | 1 cut(s) | 2023 |
| EcoRI | GAATTC | 1 cut(s) | 245 |
| EcoRII | CCWGG | 3 cut(s) | 439, 568, 2508 |
| EcoT14I | CCWWGG | 3 cut(s) | 277, 1204, 1839 |
| EcoT22I | ATGCAT | 1 cut(s) | 163 |
| ErhI | CCWWGG | 3 cut(s) | 277, 1204, 1839 |
| Esp3I | CGTCTC | 1 cut(s) | 751 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1082, 1114 |
| FaqI | GGGAC | 3 cut(s) | 2347, 2711, 2793 |
| FauI | CCCGC | 1 cut(s) | 1512 |
| FbaI | TGATCA | 3 cut(s) | 175, 268, 793 |
| FokI | GGATG | 7 cut(s) | 145, 321, 619, 866, 2399, 2567, 2775 |
| FspBI | CTAG | 5 cut(s) | 239, 1883, 1892, 2111, 2351 |
| FspI | TGCGCA | 1 cut(s) | 1590 |
| GlaI | GCGC | 2 cut(s) | 1590, 1768 |
| GsaI | CCCAGC | 2 cut(s) | 1731, 2820 |
| GsuI | CTGGAG | 6 cut(s) | 729, 999, 2270, 2531, 2621, 2770 |
| HaeIII | GGCC | 5 cut(s) | 276, 519, 1329, 1466, 1694 |
| HapII | CCGG | 2 cut(s) | 754, 1357 |
| HgaI | GACGC | 2 cut(s) | 2324, 2585 |
| HhaI | GCGC | 2 cut(s) | 1591, 1769 |
| Hin1I | GRCGYC | 1 cut(s) | 1612 |
| Hin6I | GCGC | 2 cut(s) | 1589, 1767 |
| HinP1I | GCGC | 2 cut(s) | 1589, 1767 |
| HindIII | AAGCTT | 4 cut(s) | 35, 330, 1871, 1940 |
| HpaII | CCGG | 2 cut(s) | 754, 1357 |
| HphI | GGTGA | 5 cut(s) | 241, 1549, 1705, 1855, 2089 |
| Hpy166II | GTNNAC | 6 cut(s) | 618, 971, 1801, 1919, 2080, 2213 |
| Hpy8I | GTNNAC | 6 cut(s) | 618, 971, 1801, 1919, 2080, 2213 |
| Hpy99I | CGWCG | 2 cut(s) | 480, 1614 |
| HpyAV | CCTTC | 9 cut(s) | 95, 151, 500, 709, 1479, 1716, 2286, 2346, 2750 |
| HpyCH4III | ACNGT | 7 cut(s) | 476, 688, 911, 1852, 1998, 2589, 2807 |
| HpyCH4IV | ACGT | 4 cut(s) | 1612, 1672, 2308, 2541 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 34, 224, 826, 1133, 2116, 2290 |
| HpySE526I | ACGT | 4 cut(s) | 1612, 1672, 2308, 2541 |
| Hsp92I | GRCGYC | 1 cut(s) | 1612 |
| HspAI | GCGC | 2 cut(s) | 1589, 1767 |
| Kpn2I | TCCGGA | 1 cut(s) | 753 |
| KpnI | GGTACC | 1 cut(s) | 841 |
| Ksp22I | TGATCA | 3 cut(s) | 175, 268, 793 |
| LmnI | GCTCC | 3 cut(s) | 261, 817, 2289 |
| Lsp1109I | GCAGC | 7 cut(s) | 807, 810, 838, 930, 1212, 1657, 1728 |
| LweI | GCATC | 6 cut(s) | 148, 299, 475, 1323, 2589, 2635 |
| MaeI | CTAG | 5 cut(s) | 239, 1883, 1892, 2111, 2351 |
| MaeII | ACGT | 4 cut(s) | 1612, 1672, 2308, 2541 |
| MaeIII | GTNAC | 3 cut(s) | 335, 410, 2023 |
| MbiI | CCGCTC | 1 cut(s) | 2395 |
| MfeI | CAATTG | 1 cut(s) | 1563 |
| MflI | RGATCY | 4 cut(s) | 443, 1503, 1894, 1932 |
| MhlI | GDGCHC | 1 cut(s) | 2215 |
| MlsI | TGGCCA | 1 cut(s) | 1466 |
| MluNI | TGGCCA | 1 cut(s) | 1466 |
| MlyI | GAGTC | 4 cut(s) | 84, 399, 1028, 2724 |
| MmeI | TCCRAC | 4 cut(s) | 1481, 1734, 2434, 2608 |
| Mox20I | TGGCCA | 1 cut(s) | 1466 |
| Mph1103I | ATGCAT | 1 cut(s) | 163 |
| MroI | TCCGGA | 1 cut(s) | 753 |
| MroXI | GAANNNNTTC | 3 cut(s) | 234, 346, 1705 |
| MscI | TGGCCA | 1 cut(s) | 1466 |
| MslI | CAYNNNNRTG | 6 cut(s) | 264, 870, 1565, 1908, 2075, 2540 |
| Msp20I | TGGCCA | 1 cut(s) | 1466 |
| MspA1I | CMGCKG | 4 cut(s) | 541, 799, 1731, 2290 |
| MspCI | CTTAAG | 1 cut(s) | 1898 |
| MspI | CCGG | 2 cut(s) | 754, 1357 |
| MspR9I | CCNGG | 3 cut(s) | 441, 570, 2510 |
| MunI | CAATTG | 1 cut(s) | 1563 |
| Mva1269I | GAATGC | 2 cut(s) | 328, 1903 |
| MvaI | CCWGG | 3 cut(s) | 441, 570, 2510 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 34, 224, 826, 1133, 2116, 2290 |
| NlaIV | GGNNCC | 4 cut(s) | 839, 866, 1505, 1693 |
| NmuCI | GTSAC | 1 cut(s) | 410 |
| NsbI | TGCGCA | 1 cut(s) | 1590 |
| NsiI | ATGCAT | 1 cut(s) | 163 |
| NspI | RCATGY | 3 cut(s) | 884, 1500, 2726 |
| NspV | TTCGAA | 1 cut(s) | 51 |
| OliI | CACNNNNGTG | 2 cut(s) | 870, 1565 |
| PaeI | GCATGC | 1 cut(s) | 884 |
| PagI | TCATGA | 1 cut(s) | 220 |
| PciI | ACATGT | 1 cut(s) | 1496 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 57, 2593 |
| PctI | GAATGC | 2 cut(s) | 328, 1903 |
| PdmI | GAANNNNTTC | 3 cut(s) | 234, 346, 1705 |
| PfeI | GAWTC | 8 cut(s) | 282, 342, 375, 1336, 1377, 1860, 1879, 2332 |
| PflFI | GACNNNGTC | 1 cut(s) | 408 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 70, 1963 |
| PfoI | TCCNGGA | 1 cut(s) | 439 |
| PleI | GAGTC | 4 cut(s) | 83, 399, 1027, 2723 |
| PpsI | GAGTC | 4 cut(s) | 83, 399, 1027, 2723 |
| PscI | ACATGT | 1 cut(s) | 1496 |
| PsiI | TTATAA | 1 cut(s) | 2102 |
| Psp6I | CCWGG | 3 cut(s) | 439, 568, 2508 |
| PspEI | GGTNACC | 1 cut(s) | 2023 |
| PspFI | CCCAGC | 2 cut(s) | 1727, 2816 |
| PspGI | CCWGG | 3 cut(s) | 439, 568, 2508 |
| PspN4I | GGNNCC | 4 cut(s) | 839, 866, 1505, 1693 |
| PspPI | GGNCC | 4 cut(s) | 437, 517, 1327, 1692 |
| PstI | CTGCAG | 2 cut(s) | 157, 828 |
| PstNI | CAGNNNCTG | 1 cut(s) | 446 |
| PsuI | RGATCY | 4 cut(s) | 443, 1503, 1894, 1932 |
| PsyI | GACNNNGTC | 1 cut(s) | 408 |
| PvuII | CAGCTG | 2 cut(s) | 541, 799 |
| RsaI | GTAC | 4 cut(s) | 839, 972, 1808, 2804 |
| RsaNI | GTAC | 4 cut(s) | 838, 971, 1807, 2803 |
| RseI | CAYNNNNRTG | 6 cut(s) | 264, 870, 1565, 1908, 2075, 2540 |
| Sau96I | GGNCC | 4 cut(s) | 437, 517, 1327, 1692 |
| ScaI | AGTACT | 1 cut(s) | 2804 |
| SchI | GAGTC | 4 cut(s) | 84, 399, 1028, 2724 |
| ScrFI | CCNGG | 3 cut(s) | 441, 570, 2510 |
| SduI | GDGCHC | 1 cut(s) | 2215 |
| SfaNI | GCATC | 6 cut(s) | 148, 299, 475, 1323, 2589, 2635 |
| SfcI | CTRYAG | 2 cut(s) | 153, 824 |
| SfuI | TTCGAA | 1 cut(s) | 51 |
| SinI | GGWCC | 1 cut(s) | 437 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 264, 870, 1565, 1908, 2075, 2540 |
| SmlI | CTYRAG | 5 cut(s) | 370, 1828, 1898, 1937, 2648 |
| SmoI | CTYRAG | 5 cut(s) | 370, 1828, 1898, 1937, 2648 |
| SphI | GCATGC | 1 cut(s) | 884 |
| SsiI | CCGC | 8 cut(s) | 574, 1198, 1519, 1716, 1731, 2131, 2290, 2393 |
| SspMI | CTAG | 5 cut(s) | 239, 1883, 1892, 2111, 2351 |
| StyD4I | CCNGG | 3 cut(s) | 439, 568, 2508 |
| StyI | CCWWGG | 3 cut(s) | 277, 1204, 1839 |
| TaaI | ACNGT | 7 cut(s) | 476, 688, 911, 1852, 1998, 2589, 2807 |
| TaiI | ACGT | 4 cut(s) | 1615, 1675, 2311, 2544 |
| TaqI | TCGA | 6 cut(s) | 51, 115, 186, 694, 1609, 2520 |
| TatI | WGTACW | 3 cut(s) | 970, 1806, 2802 |
| TauI | GCSGC | 3 cut(s) | 1200, 1734, 2293 |
| TfiI | GAWTC | 8 cut(s) | 282, 342, 375, 1336, 1377, 1860, 1879, 2332 |
| TscAI | CASTG | 7 cut(s) | 691, 1147, 1410, 1921, 2235, 2333, 2673 |
| TseFI | GTSAC | 1 cut(s) | 410 |
| TseI | GCWGC | 7 cut(s) | 820, 823, 826, 918, 1200, 1645, 1741 |
| Tsp45I | GTSAC | 1 cut(s) | 410 |
| TspGWI | ACGGA | 3 cut(s) | 398, 1186, 2678 |
| TspRI | CASTG | 7 cut(s) | 691, 1147, 1410, 1921, 2235, 2333, 2673 |
| Tth111I | GACNNNGTC | 1 cut(s) | 408 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 70, 1963 |
| Vha464I | CTTAAG | 1 cut(s) | 1898 |
| VneI | GTGCAC | 1 cut(s) | 2211 |
| VpaK11BI | GGWCC | 1 cut(s) | 437 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 145 |
| XapI | RAATTY | 8 cut(s) | 122, 245, 714, 943, 1605, 1979, 2516, 2642 |
| XbaI | TCTAGA | 3 cut(s) | 238, 1882, 1891 |
| XceI | RCATGY | 3 cut(s) | 884, 1500, 2726 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 284, 2227 |
| XmnI | GAANNNNTTC | 3 cut(s) | 234, 346, 1705 |
| XspI | CTAG | 5 cut(s) | 239, 1883, 1892, 2111, 2351 |
| ZraI | GACGTC | 1 cut(s) | 1613 |
| ZrmI | AGTACT | 1 cut(s) | 2804 |
| Zsp2I | ATGCAT | 1 cut(s) | 163 |