FvH4_5g33950

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001687
Physical Location & Seq
Reverse (-)
24636621 .. 24637658
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33950.t1

Sequence Viewer

Length: 903 bp
ATGGAGAAGAAGAAAGTCTCAAGACTTTGTGTAACAGGAGGTTCTGGATATATTGGCAGTAGGCTTGTCAAAAAGCTCCTGGAGAAGGGCCACACAGTCCATGCCACTCTCAAAAACTTAGAGGATGTATCAAAAGTAGGCCTTCTGAAGTCTCTCCCCAATGCAGACACCAATTTGTTGCTGTTCAGAGCTGACATATACAATCCTAATGACTTCGAACCTGCTATTGAAGGGTGTGAGTTTGTTTTTCATCTTGCAACTCCTAGGCAACATAACTCCCAAATCTCCCAGTACAAGGACACAGCTGAAGCTGCTATTGCTGGGGTGAGAAGCATTGCTGATTCTTGCATTCGGTCGCAGACAGTTAAGCGACTGATCTACACTGCAAGCATACTTTCTATGTCACCAAGAAACGAGGGTGGAGTTGGATTCAGACCCTTACTTGATGAGTCATGTTGGACACCGCTTGATGCCTCATCTACTTATGGCAATGCTTTCACAATGGGTGTGGGAGGGATTCTGGCACAACTTACTGGGGACTTGTTTTCTTGCAATGCATTAAAATTCATGCAAGAATTTATGGGATCAGTTCCTCTGGTGCACATTGATGATGTTTGCCAAGCACATATCTTCTGCATGGAACAGCCATTAATGGAAGGCAGATTCTGTTGCGCAGTTGCCCGGCACTCTATCAAAGAAATTGCAATATACTTCCAAGAAACTTACCCAGAATACAAGATACATAAGGAATTGACTGAAGGGCCTGAAGAAGGAAGCCAAAGTGATTTCAGTAAGCTGAAGAAGATGGGCTTTGAGTACAAGTATGGCATCAAGGAGATACTAGATGACAGTGTAGCATGGGCAAAGAAGTTAAATGAGAGCTCTTTTTCCCAAGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

301

Amino Acids

33.33

Weight (kDa)

6.26

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 109 4.8e-09 NmrA-like family
Epimerase PF01370 10 - 163 1.6e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 136 8.5e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 1.5e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 132 7.8e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 673
Acc36I ACCTGC 1 cut(s) 229
AciI CCGC 1 cut(s) 464
AclWI GGATC 1 cut(s) 592
AcsI RAATTY 2 cut(s) 563, 575
AcuI CTGAAG 5 cut(s) 167, 327, 777, 786, 818
AfaI GTAC 2 cut(s) 293, 818
AfiI CCNNNNNNNGG 3 cut(s) 85, 295, 770
AgsI TTSAA 1 cut(s) 230
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 6 cut(s) 76, 191, 305, 311, 796, 882
AluI AGCT 6 cut(s) 76, 191, 305, 311, 796, 882
Alw21I GWGCWC 2 cut(s) 603, 884
Alw26I GTCTC 2 cut(s) 22, 156
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 1 cut(s) 592
AlwNI CAGNNNCTG 1 cut(s) 666
AoxI GGCC 3 cut(s) 88, 139, 761
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 563, 575
AseI ATTAAT 1 cut(s) 650
AspA2I CCTAGG 1 cut(s) 263
AspLEI GCGC 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 88, 761
AsuC2I CCSGG 1 cut(s) 682
AsuHPI GGTGA 2 cut(s) 337, 396
AsuII TTCGAA 1 cut(s) 216
AvrII CCTAGG 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 603
BanII GRGCYC 1 cut(s) 884
Bbv12I GWGCWC 2 cut(s) 603, 884
BbvI GCAGC 1 cut(s) 298
BccI CCATC 1 cut(s) 799
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 682
BcoDI GTCTC 2 cut(s) 22, 156
BfaI CTAG 2 cut(s) 264, 842
BfuAI ACCTGC 1 cut(s) 229
BisI GCNGC 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 263
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 2 cut(s) 80, 682
BmgT120I GGNCC 2 cut(s) 88, 761
BmrFI CCNGG 2 cut(s) 80, 682
BmrI ACTGGG 2 cut(s) 283, 543
BmsI GCATC 2 cut(s) 460, 837
BmuI ACTGGG 2 cut(s) 283, 543
BpmI CTGGAG 1 cut(s) 101
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 4
BpuMI CCSGG 1 cut(s) 682
BsaJI CCNNGG 1 cut(s) 263
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 295, 770
Bse1I ACTGG 2 cut(s) 289, 538
Bse3DI GCAATG 3 cut(s) 333, 496, 559
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 3 cut(s) 85, 295, 770
BseMI GCAATG 3 cut(s) 333, 496, 559
BseNI ACTGG 2 cut(s) 289, 538
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 90, 141, 763
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 2 cut(s) 603, 884
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 3 cut(s) 85, 295, 770
BsmAI GTCTC 2 cut(s) 22, 156
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 3 cut(s) 90, 141, 763
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 603, 884
Bsp143I GATC 2 cut(s) 375, 584
BspACI CCGC 1 cut(s) 464
BspANI GGCC 3 cut(s) 90, 141, 763
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 1 cut(s) 592
BspT104I TTCGAA 1 cut(s) 216
BsrDI GCAATG 3 cut(s) 333, 496, 559
BsrI ACTGG 2 cut(s) 289, 538
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 2 cut(s) 375, 584
BssT1I CCWWGG 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 3 cut(s) 97, 364, 851
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 1 cut(s) 118
BstENI CCTNNNNNAGG 2 cut(s) 83, 768
BstF5I GGATG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 674
BstKTI GATC 2 cut(s) 378, 587
BstMAI GTCTC 2 cut(s) 22, 156
BstMBI GATC 2 cut(s) 375, 584
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 311, 317
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 680
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 298
BsuRI GGCC 3 cut(s) 90, 141, 763
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 856
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 388
CaiI CAGNNNCTG 1 cut(s) 666
CfoI GCGC 1 cut(s) 674
Cfr13I GGNCC 2 cut(s) 88, 761
Csp6I GTAC 2 cut(s) 292, 817
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviAII CATG 5 cut(s) 101, 453, 568, 637, 858
CviQI GTAC 2 cut(s) 292, 817
DdeI CTNAG 1 cut(s) 118
DpnI GATC 2 cut(s) 377, 586
DpnII GATC 2 cut(s) 375, 584
Ecl136II GAGCTC 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 263
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 884
Eco53kI GAGCTC 1 cut(s) 882
Eco57I CTGAAG 5 cut(s) 167, 327, 777, 786, 818
EcoICRI GAGCTC 1 cut(s) 882
EcoNI CCTNNNNNAGG 2 cut(s) 83, 768
EcoO109I RGGNCCY 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 263
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 884
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 5 cut(s) 104, 456, 571, 640, 861
FalI AAGNNNNNCTT 4 cut(s) 126, 158, 794, 826
FaqI GGGAC 1 cut(s) 551
FatI CATG 5 cut(s) 100, 452, 567, 636, 857
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 884
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 264, 842
FspI TGCGCA 1 cut(s) 673
GlaI GCGC 1 cut(s) 673
GluI GCNGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 3 cut(s) 90, 141, 763
HapII CCGG 1 cut(s) 682
HhaI GCGC 1 cut(s) 674
Hin1II CATG 5 cut(s) 104, 456, 571, 640, 861
Hin6I GCGC 1 cut(s) 672
HinP1I GCGC 1 cut(s) 672
HinfI GANTC 5 cut(s) 341, 429, 449, 517, 663
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 337, 396
Hpy166II GTNNAC 1 cut(s) 601
Hpy188I TCNGA 3 cut(s) 147, 188, 434
Hpy188III TCNNGA 2 cut(s) 21, 45
Hpy8I GTNNAC 1 cut(s) 601
HpyAV CCTTC 6 cut(s) 79, 152, 224, 650, 752, 764
HpyCH4III ACNGT 3 cut(s) 97, 364, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 317
HpyF3I CTNAG 1 cut(s) 118
Hsp92II CATG 5 cut(s) 104, 456, 571, 640, 861
HspAI GCGC 1 cut(s) 672
Kzo9I GATC 2 cut(s) 375, 584
LmnI GCTCC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 2 cut(s) 460, 837
MaeI CTAG 2 cut(s) 264, 842
MaeIII GTNAC 2 cut(s) 31, 402
MalI GATC 2 cut(s) 377, 586
MboI GATC 2 cut(s) 375, 584
MboII GAAGA 6 cut(s) 19, 22, 622, 779, 811, 814
MhlI GDGCHC 2 cut(s) 603, 884
MluCI AATT 5 cut(s) 172, 563, 575, 699, 749
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 2 cut(s) 406, 437
MnlI CCTC 6 cut(s) 32, 115, 409, 484, 506, 603
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 4 cut(s) 366, 560, 650, 872
MslI CAYNNNNRTG 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 2 cut(s) 80, 682
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 311, 317
NciI CCSGG 1 cut(s) 682
NdeII GATC 2 cut(s) 375, 584
NlaIII CATG 5 cut(s) 104, 456, 571, 640, 861
NmuCI GTSAC 1 cut(s) 402
NsbI TGCGCA 1 cut(s) 673
NsiI ATGCAT 1 cut(s) 559
NspV TTCGAA 1 cut(s) 216
PceI AGGCCT 1 cut(s) 141
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 341, 429, 517, 663
PfoI TCCNGGA 1 cut(s) 78
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 650
Psp124BI GAGCTC 1 cut(s) 884
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 78
PspPI GGNCC 2 cut(s) 88, 761
PstNI CAGNNNCTG 1 cut(s) 666
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 293, 818
RsaNI GTAC 2 cut(s) 292, 817
RseI CAYNNNNRTG 1 cut(s) 606
SacI GAGCTC 1 cut(s) 884
SaqAI TTAA 4 cut(s) 366, 560, 650, 872
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 375, 584
Sau96I GGNCC 2 cut(s) 88, 761
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 80, 682
SduI GDGCHC 2 cut(s) 603, 884
SetI ASST 8 cut(s) 43, 78, 193, 223, 307, 313, 798, 884
SfaNI GCATC 2 cut(s) 460, 837
SfuI TTCGAA 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 5 cut(s) 172, 563, 575, 699, 749
SseBI AGGCCT 1 cut(s) 141
SsiI CCGC 1 cut(s) 464
SspMI CTAG 2 cut(s) 264, 842
SstI GAGCTC 1 cut(s) 884
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 78, 680
StyI CCWWGG 1 cut(s) 263
TaaI ACNGT 3 cut(s) 97, 364, 851
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 342
TasI AATT 5 cut(s) 172, 563, 575, 699, 749
TatI WGTACW 2 cut(s) 291, 816
TfiI GAWTC 4 cut(s) 341, 429, 517, 663
Tru1I TTAA 4 cut(s) 366, 560, 650, 872
Tru9I TTAA 4 cut(s) 366, 560, 650, 872
TscAI CASTG 2 cut(s) 388, 856
TseFI GTSAC 1 cut(s) 402
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 2 cut(s) 239, 556
TspRI CASTG 2 cut(s) 388, 856
VneI GTGCAC 1 cut(s) 599
VspI ATTAAT 1 cut(s) 650
XagI CCTNNNNNAGG 2 cut(s) 83, 768
XapI RAATTY 2 cut(s) 563, 575
XmaJI CCTAGG 1 cut(s) 263
XspI CTAG 2 cut(s) 264, 842
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.