Rw6G011300

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001687
Physical Location & Seq
Forward (+)
19858486 .. 19859797
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011300.1

Sequence Viewer

Length: 777 bp
ATGGGGAAGAGTAATGGCTGCAAGGTTTGTATTACAGGAGGTGCAGGGTACATAGGCTCTTTGGCTTGTCAAGAAGCTTTTAGAGAAGGGATACACTATGATGTTTCGAAGGTGAGCCTTCTGAAGTGCCTTCCTGATTCTGATGAAAGGCTAGTGCTTTTTGAAGCTGACTTCTATAATCCAGTTACGTTTGAGCAGGCAATTGAAGGATGTGAATTTGTTTTCCATGTAGCAACTCCCTTGCTTCACACCCCAAACTCCCAGGACTATGAAGATTCAAAGACACTGGCAGAGAAAGAAATCTTGAGCTATGTGAATCGCAACAAAAGAAGTGGACTTGAGATTGTGAGCTTGGCCTGTGGTCTTGTGGGAGGGGACTCACTTCCATCATTCACGCCTGTAACTGTAGCTTGTTTAGTTTCTCAGCTTACAAATAGTGCAAATGAGTACAATTGTCTGAGATTCTTAGAGGAATTGCTAGGGAAAATTCCAATAGTCCATATTGATGATGTTTGTGATGCTCATATCTTCTGCATGGAGAGTCCTTCAATAAATGGTAGGTTTCTGTGCACAAGATCCTATGTTTCATCTGCACAAATTGCTAGTTACTATCAGCAACATTATCCAGAACTTGAAGTGAAGCAAGAGTGTTTGGATTTACCAAAGAGGCAAATTGGCTGGGGTTCTACCAAGCTTAGTGAGGAGGGATTTGTGTACAAATCTGATACGAAGATGATATTGGATGATTGCATAACGGCTGCAAGAAGATGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

258

Amino Acids

28.67

Weight (kDa)

5.02

Isoelectric Point (pI)

64.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 89 3.7e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 570
AcsI RAATTY 2 cut(s) 215, 486
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 3 cut(s) 50, 449, 716
AgsI TTSAA 5 cut(s) 164, 206, 279, 549, 635
AjnI CCWGG 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 722, 754
AluBI AGCT 7 cut(s) 77, 167, 309, 351, 410, 427, 694
AluI AGCT 7 cut(s) 77, 167, 309, 351, 410, 427, 694
Alw21I GWGCWC 1 cut(s) 572
Alw44I GTGCAC 1 cut(s) 568
AlwI GGATC 1 cut(s) 570
AoxI GGCC 1 cut(s) 354
ApaLI GTGCAC 1 cut(s) 568
ApeKI GCWGC 2 cut(s) 18, 758
ApoI RAATTY 2 cut(s) 215, 486
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 572
BbvI GCAGC 2 cut(s) 5, 745
BccI CCATC 2 cut(s) 394, 762
BceAI ACGGC 1 cut(s) 771
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 1 cut(s) 84
BfaI CTAG 3 cut(s) 152, 479, 603
BfmI CTRYAG 1 cut(s) 405
BfuI GTATCC 1 cut(s) 84
BisI GCNGC 2 cut(s) 19, 759
BlsI GCNGC 2 cut(s) 20, 760
Bme1390I CCNGG 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 508
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 325, 359
BsaJI CCNNGG 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 182, 291
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 215, 748
BseMII CTCAG 2 cut(s) 437, 449
BseNI ACTGG 2 cut(s) 182, 291
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 2 cut(s) 5, 745
BseYI CCCAGC 1 cut(s) 678
BsgI GTGCAG 2 cut(s) 63, 576
BshFI GGCC 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 572
BslFI GGGAC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 572
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 1 cut(s) 575
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 2 cut(s) 436, 450
BspPI GGATC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 182, 291
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 575
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 1 cut(s) 2
BstAPI GCANNNNNTGC 1 cut(s) 599
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 4 cut(s) 423, 458, 466, 695
BstF5I GGATG 2 cut(s) 215, 748
BstKTI GATC 1 cut(s) 578
BstMBI GATC 1 cut(s) 575
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 2 cut(s) 5, 745
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BsuI GTATCC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 356
BtsCI GGATG 2 cut(s) 215, 748
BtsIMutI CAGTG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 198
Csp6I GTAC 3 cut(s) 49, 448, 715
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 2 cut(s) 227, 535
CviQI GTAC 3 cut(s) 49, 448, 715
DdeI CTNAG 4 cut(s) 423, 458, 466, 695
DpnI GATC 1 cut(s) 577
DpnII GATC 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco57I CTGAAG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 261
FaeI CATG 2 cut(s) 230, 538
FaqI GGGAC 1 cut(s) 389
FatI CATG 2 cut(s) 226, 534
Fnu4HI GCNGC 2 cut(s) 19, 759
FokI GGATG 2 cut(s) 222, 755
Fsp4HI GCNGC 2 cut(s) 19, 759
FspBI CTAG 3 cut(s) 152, 479, 603
GluI GCNGC 2 cut(s) 19, 759
GsaI CCCAGC 1 cut(s) 682
HaeIII GGCC 1 cut(s) 356
Hin1II CATG 2 cut(s) 230, 538
HindIII AAGCTT 2 cut(s) 75, 692
HinfI GANTC 6 cut(s) 137, 275, 316, 377, 462, 541
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 335, 570, 715
Hpy188I TCNGA 4 cut(s) 123, 142, 459, 724
Hpy188III TCNNGA 4 cut(s) 71, 134, 304, 626
Hpy8I GTNNAC 3 cut(s) 335, 570, 715
HpyAV CCTTC 6 cut(s) 80, 103, 128, 140, 200, 555
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 8 cut(s) 21, 44, 440, 534, 570, 593, 750, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 4 cut(s) 423, 458, 466, 695
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 2 cut(s) 230, 538
Kzo9I GATC 1 cut(s) 575
Lsp1109I GCAGC 2 cut(s) 5, 745
LweI GCATC 1 cut(s) 508
MaeI CTAG 3 cut(s) 152, 479, 603
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 3 cut(s) 184, 400, 605
MalI GATC 1 cut(s) 577
MboI GATC 1 cut(s) 575
MboII GAAGA 5 cut(s) 19, 284, 520, 742, 777
MfeI CAATTG 2 cut(s) 201, 451
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 1 cut(s) 572
MluCI AATT 7 cut(s) 201, 215, 451, 473, 486, 597, 672
MlyI GAGTC 2 cut(s) 371, 550
MnlI CCTC 6 cut(s) 32, 365, 463, 660, 694, 697
MslI CAYNNNNRTG 2 cut(s) 99, 504
MspR9I CCNGG 1 cut(s) 263
MunI CAATTG 2 cut(s) 201, 451
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 599
NdeII GATC 1 cut(s) 575
NlaIII CATG 2 cut(s) 230, 538
NspV TTCGAA 1 cut(s) 107
PfeI GAWTC 4 cut(s) 137, 275, 316, 462
PkrI GCNGC 2 cut(s) 20, 760
PleI GAGTC 2 cut(s) 371, 549
PpsI GAGTC 2 cut(s) 371, 549
Psp6I CCWGG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 678
PspGI CCWGG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 575
RsaI GTAC 3 cut(s) 50, 449, 716
RsaNI GTAC 3 cut(s) 49, 448, 715
RseI CAYNNNNRTG 2 cut(s) 99, 504
SatI GCNGC 2 cut(s) 19, 759
Sau3AI GATC 1 cut(s) 575
SchI GAGTC 2 cut(s) 371, 550
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 572
SfaNI GCATC 1 cut(s) 508
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 99, 504
SmlI CTYRAG 2 cut(s) 304, 338
SmoI CTYRAG 2 cut(s) 304, 338
Sse9I AATT 7 cut(s) 201, 215, 451, 473, 486, 597, 672
SspMI CTAG 3 cut(s) 152, 479, 603
StyD4I CCNGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 1 cut(s) 191
TaqI TCGA 1 cut(s) 107
TasI AATT 7 cut(s) 201, 215, 451, 473, 486, 597, 672
TatI WGTACW 2 cut(s) 447, 714
TfiI GAWTC 4 cut(s) 137, 275, 316, 462
TscAI CASTG 1 cut(s) 291
TseI GCWGC 2 cut(s) 18, 758
TspDTI ATGAA 3 cut(s) 159, 285, 576
TspRI CASTG 1 cut(s) 291
VneI GTGCAC 1 cut(s) 568
XapI RAATTY 2 cut(s) 215, 486
XspI CTAG 3 cut(s) 152, 479, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.