MD06G1103900.v1.1

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001687
Physical Location & Seq
Forward (+)
24184052 .. 24186455
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1103900.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGAGAACAAAAACTCTAGAGTTTGTGTTACAGGTGCTTCTGGATTCATTGCCTCTTCACTCGTTAACAAGCTCCTACAGCAGGGCCACACAGTCCACGCCACCCTCAGGAACTTGGATGATGCGTCGAAAGTTGGGCTTCTCAAGTCCCTTCCTAATGCGGACACCAACTTGTTGCTGTTCCAAGCTGACCTATATGACCCTCTAGAGTTTGAGCCTGCCATGGAAGGCTGTGAGTTTGTTTTCCATGTTGCCACTCCGATGCAGCATAACAACCTAAGCTCTCAGTACAAGGACACAGCTGAAGCTGCTGTTGCTGGGGTGAGAATCATTGCTGATTCCTGCATTCGTTCGCAGACCGTCAAGCGACTCATCTACACTGCAAGCGTACTAGCTGCGTCGCCTCGGACAGAAGATGGGGCTGGATTCAAACCCTACTTTGATGAATCATGTTGGACACCCCTTGATGTCTCATTTCCTCATGGCACTGATTTCACAATGGGGTATATCGTATCAAAGACATTAGCAGAGAAAGCAGTGCTGAGCTATAACGACATTGATGGTGGTAAATTAGAAGCAGTGACTCTTCCTTGTGGCCTAGTAGGAGGAGAAACGCTTCTCCCAAATTCGCCTCTAAGTGTGGGAGTAGTTATATCACAACTTACCGGGGACTTGAATGGTTACAATGCATTAAAATTCATGCAAGAAGTTATCGGTTCTGTTCCTCTTGTACACATTGAAGATGTTTGCCGAGGACATATCTTCTGCATGGAACAACCATCGATGAGAGGCAGATTCTGCTGTGCAGTTGCCAGTCCTAGTATCAAAGAAATCGCTGCCTTCTTCCAAGAAAACTACCCGGAATACAAGATAGCAAAAGAATTCGCCGAAGGACCAGAAGAAGGAATCAAATGTGACTTTACTAAGCTGGTGAAGATCGGTTTTGAGTACAAGTATGGCGTGAAGAACATACTTGATGACAGTGTAGCAAGTGGAAGGAGGTTATCAGAGAGCTCTCTTTCTCAACTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

345

Amino Acids

37.44

Weight (kDa)

5.17

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 108 1.9e-10 NmrA-like family
Epimerase PF01370 8 - 261 6.9e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 201 1.7e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 119 4.3e-07 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 134 2.3e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 161
AcsI RAATTY 3 cut(s) 625, 695, 881
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 4 cut(s) 290, 390, 732, 950
AfiI CCNNNNNNNGG 3 cut(s) 82, 108, 902
AgsI TTSAA 3 cut(s) 430, 676, 740
AluBI AGCT 9 cut(s) 73, 188, 282, 302, 308, 395, 546, 928, 1014
AluI AGCT 9 cut(s) 73, 188, 282, 302, 308, 395, 546, 928, 1014
Alw21I GWGCWC 1 cut(s) 1016
Alw26I GTCTC 1 cut(s) 475
AlwNI CAGNNNCTG 1 cut(s) 798
AoxI GGCC 2 cut(s) 85, 595
ApeKI GCWGC 4 cut(s) 265, 308, 395, 836
ApoI RAATTY 3 cut(s) 625, 695, 881
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 85, 893
AsuC2I CCSGG 2 cut(s) 667, 860
AsuHPI GGTGA 2 cut(s) 334, 943
AvaII GGWCC 1 cut(s) 893
AxyI CCTNAGG 1 cut(s) 107
BanII GRGCYC 1 cut(s) 1016
Bbv12I GWGCWC 1 cut(s) 1016
BbvI GCAGC 4 cut(s) 277, 295, 382, 823
BccI CCATC 3 cut(s) 410, 554, 787
BcnI CCSGG 2 cut(s) 667, 860
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 5 cut(s) 18, 206, 392, 599, 819
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 4 cut(s) 266, 309, 396, 837
BlpI GCTNAGC 1 cut(s) 542
BlsI GCNGC 4 cut(s) 267, 310, 397, 838
Bme1390I CCNGG 2 cut(s) 667, 860
Bme18I GGWCC 1 cut(s) 893
BmgT120I GGNCC 2 cut(s) 85, 893
BmrFI CCNGG 2 cut(s) 667, 860
BmsI GCATC 2 cut(s) 112, 252
Bpu10I CCTNAGC 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 667, 860
Bsa29I ATCGAT 1 cut(s) 782
BsaJI CCNNGG 4 cut(s) 222, 404, 666, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 108, 902
Bse1I ACTGG 1 cut(s) 813
Bse21I CCTNAGG 1 cut(s) 107
Bse3DI GCAATG 2 cut(s) 48, 330
BseCI ATCGAT 1 cut(s) 782
BseDI CCNNGG 4 cut(s) 222, 404, 666, 751
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 3 cut(s) 82, 108, 902
BseMI GCAATG 2 cut(s) 48, 330
BseMII CTCAG 3 cut(s) 121, 299, 533
BseNI ACTGG 1 cut(s) 813
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 4 cut(s) 277, 295, 382, 823
BseYI CCCAGC 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 825
BshFI GGCC 2 cut(s) 87, 597
BshVI ATCGAT 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 1016
BsiSI CCGG 2 cut(s) 666, 860
BslFI GGGAC 2 cut(s) 133, 683
BslI CCNNNNNNNGG 3 cut(s) 82, 108, 902
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 2 cut(s) 133, 683
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 2 cut(s) 87, 597
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 1 cut(s) 936
Bsp1720I GCTNAGC 1 cut(s) 542
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 1 cut(s) 161
BspANI GGCC 2 cut(s) 87, 597
BspCNI CTCAG 3 cut(s) 120, 298, 534
BspDI ATCGAT 1 cut(s) 782
BsrDI GCAATG 2 cut(s) 48, 330
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 1 cut(s) 813
BssECI CCNNGG 4 cut(s) 222, 404, 666, 751
BssMI GATC 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 222
Bst4CI ACNGT 3 cut(s) 94, 361, 983
Bst6I CTCTTC 2 cut(s) 61, 591
BstAPI GCANNNNNTGC 1 cut(s) 798
BstAUI TGTACA 1 cut(s) 730
BstC8I GCNNGC 2 cut(s) 219, 385
BstDEI CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
BstDSI CCRYGG 1 cut(s) 222
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 939
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 1 cut(s) 936
BstMWI GCNNNNNNNGC 5 cut(s) 79, 308, 314, 533, 798
BstSCI CCNGG 2 cut(s) 665, 858
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 4 cut(s) 277, 295, 382, 823
Bsu15I ATCGAT 1 cut(s) 782
Bsu36I CCTNAGG 1 cut(s) 107
BsuRI GGCC 2 cut(s) 87, 597
BsuTUI ATCGAT 1 cut(s) 782
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 3 cut(s) 378, 543, 585
BtsIMutI CAGTG 5 cut(s) 378, 486, 543, 585, 988
Cac8I GCNNGC 2 cut(s) 219, 385
CaiI CAGNNNCTG 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 85, 893
ClaI ATCGAT 1 cut(s) 782
CseI GACGC 2 cut(s) 114, 387
Csp6I GTAC 4 cut(s) 289, 389, 731, 949
CviAII CATG 6 cut(s) 223, 248, 450, 482, 700, 769
CviQI GTAC 4 cut(s) 289, 389, 731, 949
DdeI CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
DpnI GATC 1 cut(s) 938
DpnII GATC 1 cut(s) 936
Eam1104I CTCTTC 2 cut(s) 61, 591
EarI CTCTTC 2 cut(s) 61, 591
Ecl136II GAGCTC 1 cut(s) 1014
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 1016
Eco47I GGWCC 1 cut(s) 893
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 1014
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 881
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 6 cut(s) 226, 251, 453, 485, 703, 772
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 2 cut(s) 133, 683
FatI CATG 6 cut(s) 222, 247, 449, 481, 699, 768
Fnu4HI GCNGC 4 cut(s) 266, 309, 396, 837
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 4 cut(s) 266, 309, 396, 837
FspBI CTAG 5 cut(s) 18, 206, 392, 599, 819
GluI GCNGC 4 cut(s) 266, 309, 396, 837
GsaI CCCAGC 1 cut(s) 321
HaeIII GGCC 2 cut(s) 87, 597
HapII CCGG 2 cut(s) 666, 860
HgaI GACGC 2 cut(s) 114, 387
Hin1II CATG 6 cut(s) 226, 251, 453, 485, 703, 772
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 9 cut(s) 45, 327, 338, 369, 426, 446, 583, 795, 906
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 2 cut(s) 666, 860
HphI GGTGA 2 cut(s) 334, 943
Hpy166II GTNNAC 3 cut(s) 67, 97, 733
Hpy188I TCNGA 3 cut(s) 261, 408, 1009
Hpy188III TCNNGA 4 cut(s) 18, 42, 109, 206
Hpy8I GTNNAC 3 cut(s) 67, 97, 733
Hpy99I CGWCG 2 cut(s) 130, 403
HpyAV CCTTC 6 cut(s) 161, 221, 850, 884, 896, 990
HpyCH4III ACNGT 3 cut(s) 94, 361, 983
HpyCH4V TGCA 7 cut(s) 265, 345, 383, 689, 703, 768, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 308, 314, 533, 798
HpyF3I CTNAG 6 cut(s) 107, 278, 285, 542, 635, 924
Hsp92II CATG 6 cut(s) 226, 251, 453, 485, 703, 772
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 936
LmnI GCTCC 1 cut(s) 78
Lsp1109I GCAGC 4 cut(s) 277, 295, 382, 823
LweI GCATC 2 cut(s) 112, 252
MaeI CTAG 5 cut(s) 18, 206, 392, 599, 819
MaeIII GTNAC 4 cut(s) 28, 580, 680, 914
MalI GATC 1 cut(s) 938
MboI GATC 1 cut(s) 936
MboII GAAGA 9 cut(s) 48, 425, 578, 752, 754, 835, 911, 946, 976
MhlI GDGCHC 1 cut(s) 1016
MluCI AATT 4 cut(s) 569, 625, 695, 881
MlyI GAGTC 2 cut(s) 363, 577
MmeI TCCRAC 1 cut(s) 434
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 3 cut(s) 66, 692, 1033
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 2 cut(s) 666, 860
MspR9I CCNGG 2 cut(s) 667, 860
Mva1269I GAATGC 1 cut(s) 345
MwoI GCNNNNNNNGC 5 cut(s) 79, 308, 314, 533, 798
NciI CCSGG 2 cut(s) 667, 860
NcoI CCATGG 1 cut(s) 222
NdeII GATC 1 cut(s) 936
NlaIII CATG 6 cut(s) 226, 251, 453, 485, 703, 772
NmeAIII GCCGAG 1 cut(s) 776
NmuCI GTSAC 2 cut(s) 580, 914
NsiI ATGCAT 1 cut(s) 691
PctI GAATGC 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 7 cut(s) 45, 327, 338, 426, 446, 795, 906
PkrI GCNGC 4 cut(s) 267, 310, 397, 838
PleI GAGTC 2 cut(s) 363, 577
PpsI GAGTC 2 cut(s) 363, 577
Psp124BI GAGCTC 1 cut(s) 1016
PspFI CCCAGC 1 cut(s) 317
PspPI GGNCC 2 cut(s) 85, 893
PstNI CAGNNNCTG 1 cut(s) 798
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 4 cut(s) 290, 390, 732, 950
RsaNI GTAC 4 cut(s) 289, 389, 731, 949
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 1016
SaqAI TTAA 3 cut(s) 66, 692, 1033
SatI GCNGC 4 cut(s) 266, 309, 396, 837
Sau3AI GATC 1 cut(s) 936
Sau96I GGNCC 2 cut(s) 85, 893
SchI GAGTC 2 cut(s) 363, 577
ScrFI CCNGG 2 cut(s) 667, 860
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 2 cut(s) 112, 252
SfcI CTRYAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 4 cut(s) 569, 625, 695, 881
SsiI CCGC 1 cut(s) 161
SspMI CTAG 5 cut(s) 18, 206, 392, 599, 819
SstI GAGCTC 1 cut(s) 1016
StyD4I CCNGG 2 cut(s) 665, 858
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 94, 361, 983
TaqI TCGA 2 cut(s) 128, 782
TasI AATT 4 cut(s) 569, 625, 695, 881
TatI WGTACW 3 cut(s) 288, 730, 948
TfiI GAWTC 7 cut(s) 45, 327, 338, 426, 446, 795, 906
Tru1I TTAA 3 cut(s) 66, 692, 1033
Tru9I TTAA 3 cut(s) 66, 692, 1033
TscAI CASTG 5 cut(s) 385, 493, 543, 585, 988
TseFI GTSAC 2 cut(s) 580, 914
TseI GCWGC 4 cut(s) 265, 308, 395, 836
Tsp45I GTSAC 2 cut(s) 580, 914
TspDTI ATGAA 3 cut(s) 37, 459, 688
TspRI CASTG 5 cut(s) 385, 493, 543, 585, 988
VpaK11BI GGWCC 1 cut(s) 893
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 3 cut(s) 625, 695, 881
XbaI TCTAGA 2 cut(s) 17, 205
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 5 cut(s) 18, 206, 392, 599, 819
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.