MD01G1077900.v1.1

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001687
Physical Location & Seq
Forward (+)
18442448 .. 18445536
3089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1077900.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGAGAACACTAGGGTTTGCGTTACTGGAGGCTCAAGCTACCTCGGCTCTTCACTTATCAAAGGGCTCTTAGAGAAAGGATACACCGTCCATGCAACCCTAAGAAATCTGGGTGATCCATTTAAAGTGGGACTTCTCAAGGGTCTTCCTACTCCTGGTGCAGACAGCAGACTGAAATTGTTTGAAGCAGATATATATAATCCTGATGAGTTTGAGACAGCAATTCAAGGGTGCAACTATATTTTTCACTTGGCCACCCCTTTGCAGCACGAGCCCAAGGATACTCAGTTCAAAGACACTTCTGAAGCTGCAGTTTCGGGAATTAAGAGCATAGTGAGGTCTTGTATCAAATCAGGCACCGTAAAGCGGCTCATTTACACAGCCTCTGTGGTGGCCGCTTCACCAATCAAAGACTCTGGAAATGGCTTCAAACAGTTCATGGATGAGTCCTGTTGGACACCCCTCAATCTTTCATTTGCCTTTGCCAATAAAGTATTGATTGACTATACACACTCAAAGAGCCTTTCAGAGAAAGAAGTGTTGAAGCACACTAGTGGTGACCTTGAAGTAGTCAGCATTGCCTGTGGCCTTGTGGGAGGGGACACACTTCTCACTGCTATGCCTGAGAGCATGGGAGTGCTCATTTCACAAATCACAAAAGATAGTAGGAGATACCGAACCCTGAGATTTTTGGAGGAATTGCTTGGTAAAGTTCCAATTGTGCACATTGAAGATGTTTGTGAGGCACACATATTATGCATGGAGAAGCCTTCCATCAATGGCAGATTTCTATGTGTTAGTGCATATTTGTCTTCAGCAGAAATAGCATCTCACTGGGAAAAAAACTACCCAGGTCTCAGAATAGCTGAAGAGTTTGTGGAAGATTCGGGGAGAGACATTGGCTGGGGTTCCACAAAGCTGAAGAAAATTGGATTTGAGTACAAATTTGATGCCAAGGTGATATTAGATGACACTCTAAAGTGGGCCCAAAAGATGGGTTTCAGCCAAAATACTGTTGTACCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

343

Amino Acids

37.7

Weight (kDa)

6.72

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 128 6e-07 NmrA-like family
Epimerase PF01370 6 - 257 1.2e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 8 - 182 6.2e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 8 - 110 4.4e-07 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 8 - 207 2.7e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 994
AciI CCGC 2 cut(s) 367, 396
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 2 cut(s) 252, 393
AcsI RAATTY 1 cut(s) 944
AcuI CTGAAG 4 cut(s) 324, 798, 888, 941
AfaI GTAC 2 cut(s) 941, 1020
AfiI CCNNNNNNNGG 3 cut(s) 155, 366, 994
AgsI TTSAA 7 cut(s) 185, 227, 292, 430, 544, 566, 731
AhlI ACTAGT 1 cut(s) 551
AjnI CCWGG 2 cut(s) 154, 850
AleI CACNNNNGTG 1 cut(s) 552
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 4 cut(s) 39, 308, 866, 919
AluI AGCT 4 cut(s) 39, 308, 866, 919
Alw21I GWGCWC 2 cut(s) 642, 726
Alw26I GTCTC 3 cut(s) 209, 860, 888
Alw44I GTGCAC 1 cut(s) 722
AlwI GGATC 1 cut(s) 110
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 4 cut(s) 252, 393, 586, 984
ApaI GGGCCC 1 cut(s) 988
ApaLI GTGCAC 1 cut(s) 722
ApeKI GCWGC 2 cut(s) 265, 308
ApoI RAATTY 1 cut(s) 944
AspS9I GGNCC 2 cut(s) 984, 985
AsuHPI GGTGA 4 cut(s) 125, 393, 569, 970
BaeGI GKGCMC 2 cut(s) 726, 988
BaeI ACNNNNGTAYC 1 cut(s) 1002
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 3 cut(s) 69, 276, 988
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 137, 804
Bbv12I GWGCWC 2 cut(s) 642, 726
BbvI GCAGC 2 cut(s) 277, 295
BccI CCATC 2 cut(s) 782, 988
BciT130I CCWGG 2 cut(s) 156, 852
BciVI GTATCC 2 cut(s) 74, 274
BcoDI GTCTC 3 cut(s) 209, 860, 888
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 2 cut(s) 12, 552
BfmI CTRYAG 1 cut(s) 309
BfuI GTATCC 2 cut(s) 74, 274
BisI GCNGC 4 cut(s) 266, 309, 368, 396
BlsI GCNGC 4 cut(s) 267, 310, 369, 397
Bme1390I CCNGG 2 cut(s) 156, 852
BmgT120I GGNCC 2 cut(s) 984, 985
BmiI GGNNCC 3 cut(s) 358, 910, 986
BmrFI CCNGG 2 cut(s) 156, 852
BmrI ACTGGG 1 cut(s) 844
BmsI GCATC 2 cut(s) 836, 940
BmuI ACTGGG 1 cut(s) 844
BpiI GAAGAC 2 cut(s) 137, 804
BpmI CTGGAG 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 19, 122
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 4 cut(s) 43, 276, 850, 954
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 366, 994
Bse1I ACTGG 2 cut(s) 31, 839
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 2 cut(s) 156, 852
BseDI CCNNGG 4 cut(s) 43, 276, 850, 954
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 3 cut(s) 155, 366, 994
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 4 cut(s) 299, 615, 674, 871
BseNI ACTGG 2 cut(s) 31, 839
BseSI GKGCMC 2 cut(s) 726, 988
BseXI GCAGC 2 cut(s) 277, 295
BseYI CCCAGC 1 cut(s) 903
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 4 cut(s) 254, 395, 588, 986
BshNI GGYRCC 1 cut(s) 356
BsiHKAI GWGCWC 2 cut(s) 642, 726
BslFI GGGAC 2 cut(s) 144, 614
BslI CCNNNNNNNGG 3 cut(s) 155, 366, 994
BsmAI GTCTC 3 cut(s) 209, 860, 888
BsmFI GGGAC 2 cut(s) 144, 614
BsnI GGCC 4 cut(s) 254, 395, 588, 986
Bso31I GGTCTC 1 cut(s) 860
Bsp120I GGGCCC 1 cut(s) 984
Bsp1286I GDGCHC 5 cut(s) 69, 276, 642, 726, 988
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 2 cut(s) 367, 396
BspANI GGCC 4 cut(s) 254, 395, 588, 986
BspCNI CTCAG 4 cut(s) 298, 616, 675, 870
BspLI GGNNCC 3 cut(s) 358, 910, 986
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 110
BspQI GCTCTTC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 860
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 2 cut(s) 31, 839
BssECI CCNNGG 4 cut(s) 43, 276, 850, 954
BssMI GATC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 276, 954
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 156, 852
Bst4CI ACNGT 4 cut(s) 88, 361, 435, 1015
Bst6I CTCTTC 2 cut(s) 55, 864
BstDEI CTNAG 6 cut(s) 70, 101, 285, 624, 683, 857
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 1 cut(s) 448
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 3 cut(s) 209, 860, 888
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 45, 271, 824
BstNI CCWGG 2 cut(s) 156, 852
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 154, 850
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 2 cut(s) 726, 988
BstV1I GCAGC 2 cut(s) 277, 295
BstV2I GAAGAC 2 cut(s) 137, 804
BsuI GTATCC 2 cut(s) 74, 274
BsuRI GGCC 4 cut(s) 254, 395, 588, 986
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 2 cut(s) 612, 832
CaiI CAGNNNCTG 1 cut(s) 386
Cfr13I GGNCC 2 cut(s) 984, 985
Csp6I GTAC 2 cut(s) 940, 1019
CviAII CATG 4 cut(s) 92, 439, 631, 760
CviQI GTAC 2 cut(s) 940, 1019
DdeI CTNAG 6 cut(s) 70, 101, 285, 624, 683, 857
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 2 cut(s) 252, 393
Eam1104I CTCTTC 2 cut(s) 55, 864
EarI CTCTTC 2 cut(s) 55, 864
Eco130I CCWWGG 2 cut(s) 276, 954
Eco24I GRGCYC 3 cut(s) 69, 276, 988
Eco31I GGTCTC 1 cut(s) 860
Eco57I CTGAAG 4 cut(s) 324, 798, 888, 941
Eco91I GGTNACC 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 154, 850
EcoT14I CCWWGG 2 cut(s) 276, 954
EcoT22I ATGCAT 1 cut(s) 761
EcoT38I GRGCYC 3 cut(s) 69, 276, 988
ErhI CCWWGG 2 cut(s) 276, 954
FaeI CATG 4 cut(s) 95, 442, 634, 763
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 2 cut(s) 144, 614
FatI CATG 4 cut(s) 91, 438, 630, 759
Fnu4HI GCNGC 4 cut(s) 266, 309, 368, 396
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 3 cut(s) 69, 276, 988
Fsp4HI GCNGC 4 cut(s) 266, 309, 368, 396
FspBI CTAG 2 cut(s) 12, 552
GluI GCNGC 4 cut(s) 266, 309, 368, 396
GsaI CCCAGC 1 cut(s) 907
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 4 cut(s) 254, 395, 588, 986
Hin1II CATG 4 cut(s) 95, 442, 634, 763
HinfI GANTC 3 cut(s) 413, 446, 884
HphI GGTGA 4 cut(s) 125, 393, 569, 970
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 3 cut(s) 304, 529, 860
Hpy188III TCNNGA 3 cut(s) 203, 318, 417
Hpy8I GTNNAC 1 cut(s) 724
HpyAV CCTTC 1 cut(s) 780
HpyCH4III ACNGT 4 cut(s) 88, 361, 435, 1015
HpyCH4V TGCA 8 cut(s) 95, 161, 234, 265, 311, 724, 759, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 271, 824
HpyF3I CTNAG 6 cut(s) 70, 101, 285, 624, 683, 857
Hsp92II CATG 4 cut(s) 95, 442, 634, 763
Kzo9I GATC 1 cut(s) 115
LguI GCTCTTC 1 cut(s) 55
Lsp1109I GCAGC 2 cut(s) 277, 295
LweI GCATC 2 cut(s) 836, 940
MaeI CTAG 2 cut(s) 12, 552
MaeIII GTNAC 2 cut(s) 22, 557
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 7 cut(s) 42, 137, 743, 804, 881, 893, 934
MfeI CAATTG 1 cut(s) 717
MhlI GDGCHC 5 cut(s) 69, 276, 642, 726, 988
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 7 cut(s) 176, 222, 321, 698, 717, 927, 944
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 2 cut(s) 407, 455
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 8 cut(s) 23, 53, 330, 394, 473, 590, 688, 736
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 761
MscI TGGCCA 1 cut(s) 254
MseI TTAA 2 cut(s) 123, 324
MslI CAYNNNNRTG 3 cut(s) 552, 617, 635
Msp20I TGGCCA 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 156, 852
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 2 cut(s) 156, 852
MwoI GCNNNNNNNGC 3 cut(s) 45, 271, 824
NdeII GATC 1 cut(s) 115
NlaIII CATG 4 cut(s) 95, 442, 634, 763
NlaIV GGNNCC 3 cut(s) 358, 910, 986
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 557
NsiI ATGCAT 1 cut(s) 761
OliI CACNNNNGTG 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 884
PflMI CCANNNNNTGG 1 cut(s) 994
PkrI GCNGC 4 cut(s) 267, 310, 369, 397
PleI GAGTC 2 cut(s) 407, 454
PpsI GAGTC 2 cut(s) 407, 454
Psp6I CCWGG 2 cut(s) 154, 850
PspEI GGTNACC 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 903
PspGI CCWGG 2 cut(s) 154, 850
PspN4I GGNNCC 3 cut(s) 358, 910, 986
PspOMI GGGCCC 1 cut(s) 984
PspPI GGNCC 2 cut(s) 984, 985
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 941, 1020
RsaNI GTAC 2 cut(s) 940, 1019
RseI CAYNNNNRTG 3 cut(s) 552, 617, 635
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 2 cut(s) 123, 324
SatI GCNGC 4 cut(s) 266, 309, 368, 396
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 2 cut(s) 984, 985
SchI GAGTC 2 cut(s) 407, 455
ScrFI CCNGG 2 cut(s) 156, 852
SduI GDGCHC 5 cut(s) 69, 276, 642, 726, 988
SetI ASST 9 cut(s) 41, 45, 310, 341, 564, 856, 868, 921, 960
SfaNI GCATC 2 cut(s) 836, 940
SfcI CTRYAG 1 cut(s) 309
SmiMI CAYNNNNRTG 3 cut(s) 552, 617, 635
SmlI CTYRAG 2 cut(s) 34, 137
SmoI CTYRAG 2 cut(s) 34, 137
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 7 cut(s) 176, 222, 321, 698, 717, 927, 944
SsiI CCGC 2 cut(s) 367, 396
SspMI CTAG 2 cut(s) 12, 552
StyD4I CCNGG 2 cut(s) 154, 850
StyI CCWWGG 2 cut(s) 276, 954
TaaI ACNGT 4 cut(s) 88, 361, 435, 1015
TasI AATT 7 cut(s) 176, 222, 321, 698, 717, 927, 944
TatI WGTACW 1 cut(s) 939
TauI GCSGC 2 cut(s) 370, 398
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 2 cut(s) 123, 324
Tru9I TTAA 2 cut(s) 123, 324
TscAI CASTG 2 cut(s) 619, 839
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 2 cut(s) 265, 308
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 427, 462
TspRI CASTG 2 cut(s) 619, 839
Van91I CCANNNNNTGG 1 cut(s) 994
VneI GTGCAC 1 cut(s) 722
XapI RAATTY 1 cut(s) 944
XspI CTAG 2 cut(s) 12, 552
Zsp2I ATGCAT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.