pycom07g14620

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001687
Physical Location & Seq
Forward (+)
16574558 .. 16583819
9262 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14620.1

Sequence Viewer

Length: 1026 bp
ATGGAGTGCTGCAGGGTTTGTGTTACAGGAGCCTCGAGCTACCTTGGTTCTTCACTTGTCAAAAGGCTCTTGGAGAAAGGATACACAGTCCATGCAACACTGAGAAATCTTGGTGATCCATTTAAAGTGGGACTTGTCCAAAGTCTTCCTAAAGCAGACAACAGACTGAAATTGTTTGAAGCAGATATATATAGTCCTTATGACTTTGAACAAGCAATTCAAGGCTGCAACTATATTTTTCACTTGGCCACCCCTTTGCAGAATGAGCCCAAGGATTATCAGTTCAAGGACATATCTGAAGCTGCAGTTTCTGGGCTTAACAGCATAGTGAAGTCTTCCATCAAATCAGGCACCATAAAGCGATTCATTTACACAGCCTCCGTGGTAGCTGCATCACCAGTCAAAGATTCTGGAAACGGCTTCAAACAATTCATGGATGAGTCCTGTTGGACTCCCCTTAATCTTTCAATTCCTTTTGCCACACAACCAATGATGGAGTATACACACTCAAAGATCCTTTCAGAGAAAGAAGCGCTGAAGCACATTAGTGATGAGCTTGAAGTAGTTAGCATTGTCTGTGGCCTTGTGGGAGGGGATACATTTCTCCCTACTATGCCTAACAGCATGAAAGTGCTCATTTCTCAGATCACAAAAAATGAGAGGAGGTACAAAACCCTGAGATTTTTGGAGGAATTGTTGGGTAAACTTCCATTTGTTCACATTGAAGATGTCTGTACGGCACACATATTATGCATGGAGCAGCCTTCCATCAGCGGCAGATTTCTATGTGCCGGTGCATATTTGTCATCAGCAGAAATAGCATCTCACTGGGAGAAAAATTATCCAGATATCAGAATTGCTAAAGAGTTTGTTGAAGATATAGAGAGAGAAATTTATTGGGGTTCTTCAAAGCTGAAGAAAATTGGATTCAAGTACAAATTTGGTGCCAAGGAGATATTGGATGGAACTCTAAAGTGGGCACAAAAGATGGGTGAGTATGCTTCCAGCCAAGACCAAAGCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

342

Amino Acids

38.25

Weight (kDa)

7.03

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 127 4.9e-07 NmrA-like family
Epimerase PF01370 6 - 255 1.1e-18 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 8 - 180 3.2e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 8 - 84 1.5e-06 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 10 - 136 1.4e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 350, 944
AccI GTMKAC 1 cut(s) 500
AciI CCGC 1 cut(s) 774
AclWI GGATC 2 cut(s) 110, 508
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 2 cut(s) 891, 938
AcuI CTGAAG 3 cut(s) 318, 557, 935
AfaI GTAC 3 cut(s) 668, 736, 935
AfeI AGCGCT 1 cut(s) 534
AleI CACNNNNGTG 1 cut(s) 546
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 5 cut(s) 39, 302, 389, 556, 913
AluI AGCT 5 cut(s) 39, 302, 389, 556, 913
Alw21I GWGCWC 1 cut(s) 636
AlwI GGATC 2 cut(s) 110, 508
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 34
Aor51HI AGCGCT 1 cut(s) 534
AoxI GGCC 2 cut(s) 246, 580
ApeKI GCWGC 5 cut(s) 9, 225, 302, 389, 760
ApoI RAATTY 2 cut(s) 891, 938
AspLEI GCGC 1 cut(s) 535
AsuHPI GGTGA 3 cut(s) 125, 387, 1004
AvaI CYCGRG 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 982
BalI TGGCCA 1 cut(s) 248
BanI GGYRCC 2 cut(s) 350, 944
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 137, 327
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 4 cut(s) 212, 289, 376, 772
BccI CCATC 5 cut(s) 347, 487, 776, 956, 982
BceAI ACGGC 2 cut(s) 433, 753
BciVI GTATCC 2 cut(s) 74, 589
BfmI CTRYAG 2 cut(s) 10, 303
BfoI RGCGCY 1 cut(s) 536
BfuI GTATCC 2 cut(s) 74, 589
BisI GCNGC 6 cut(s) 10, 226, 303, 390, 761, 775
BlsI GCNGC 6 cut(s) 11, 227, 304, 391, 762, 776
BmeT110I CYCGRG 1 cut(s) 34
BmiI GGNNCC 3 cut(s) 31, 352, 946
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 2 cut(s) 401, 830
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 2 cut(s) 137, 327
BsaJI CCNNGG 4 cut(s) 43, 270, 381, 948
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 680, 710
Bse118I RCCGGY 1 cut(s) 791
Bse1I ACTGG 2 cut(s) 398, 833
BseDI CCNNGG 4 cut(s) 43, 270, 381, 948
BseGI GGATG 2 cut(s) 442, 967
BseMII CTCAG 3 cut(s) 92, 656, 668
BseNI ACTGG 2 cut(s) 398, 833
BseRI GAGGAG 1 cut(s) 676
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 4 cut(s) 212, 289, 376, 772
BshFI GGCC 2 cut(s) 248, 582
BshNI GGYRCC 2 cut(s) 350, 944
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 1 cut(s) 792
BslFI GGGAC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 2 cut(s) 248, 582
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 270, 636, 982
Bsp143I GATC 3 cut(s) 115, 513, 645
BspACI CCGC 1 cut(s) 774
BspANI GGCC 2 cut(s) 248, 582
BspCNI CTCAG 3 cut(s) 93, 655, 669
BspLI GGNNCC 3 cut(s) 31, 352, 946
BspMAI CTGCAG 2 cut(s) 14, 307
BspPI GGATC 2 cut(s) 110, 508
BspT107I GGYRCC 2 cut(s) 350, 944
BsrFI RCCGGY 1 cut(s) 791
BsrI ACTGG 2 cut(s) 398, 833
BssAI RCCGGY 1 cut(s) 791
BssECI CCNNGG 4 cut(s) 43, 270, 381, 948
BssMI GATC 3 cut(s) 115, 513, 645
BssNAI GTATAC 1 cut(s) 501
BssT1I CCWWGG 3 cut(s) 43, 270, 948
Bst1107I GTATAC 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 88
BstDEI CTNAG 3 cut(s) 101, 642, 677
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 2 cut(s) 442, 967
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 3 cut(s) 118, 516, 648
BstMBI GATC 3 cut(s) 115, 513, 645
BstMWI GCNNNNNNNGC 2 cut(s) 265, 818
BstSFI CTRYAG 2 cut(s) 10, 303
BstSLI GKGCMC 1 cut(s) 982
BstV1I GCAGC 4 cut(s) 212, 289, 376, 772
BstV2I GAAGAC 2 cut(s) 137, 327
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
BstZ17I GTATAC 1 cut(s) 501
BsuI GTATCC 2 cut(s) 74, 589
BsuRI GGCC 2 cut(s) 248, 582
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 2 cut(s) 442, 967
BtsIMutI CAGTG 2 cut(s) 98, 826
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 791
Csp6I GTAC 3 cut(s) 667, 735, 934
CviAII CATG 4 cut(s) 92, 433, 625, 754
CviQI GTAC 3 cut(s) 667, 735, 934
DdeI CTNAG 3 cut(s) 101, 642, 677
DpnI GATC 3 cut(s) 117, 515, 647
DpnII GATC 3 cut(s) 115, 513, 645
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 246
Eco130I CCWWGG 3 cut(s) 43, 270, 948
Eco24I GRGCYC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 850
Eco47III AGCGCT 1 cut(s) 534
Eco57I CTGAAG 3 cut(s) 318, 557, 935
Eco88I CYCGRG 1 cut(s) 34
EcoRV GATATC 1 cut(s) 850
EcoT14I CCWWGG 3 cut(s) 43, 270, 948
EcoT22I ATGCAT 1 cut(s) 755
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 3 cut(s) 43, 270, 948
FaeI CATG 4 cut(s) 95, 436, 628, 757
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 1 cut(s) 144
FatI CATG 4 cut(s) 91, 432, 624, 753
FblI GTMKAC 1 cut(s) 500
Fnu4HI GCNGC 6 cut(s) 10, 226, 303, 390, 761, 775
FokI GGATG 2 cut(s) 449, 974
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 6 cut(s) 10, 226, 303, 390, 761, 775
GlaI GCGC 1 cut(s) 534
GluI GCNGC 6 cut(s) 10, 226, 303, 390, 761, 775
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 2 cut(s) 248, 582
HapII CCGG 1 cut(s) 792
HhaI GCGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 95, 436, 628, 757
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HinfI GANTC 5 cut(s) 363, 407, 440, 451, 927
HpaII CCGG 1 cut(s) 792
HphI GGTGA 3 cut(s) 125, 387, 1004
Hpy166II GTNNAC 3 cut(s) 501, 704, 718
Hpy188I TCNGA 5 cut(s) 298, 523, 645, 854, 1025
Hpy188III TCNNGA 2 cut(s) 411, 845
Hpy8I GTNNAC 3 cut(s) 501, 704, 718
HpyAV CCTTC 1 cut(s) 774
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4V TGCA 8 cut(s) 12, 95, 228, 259, 305, 392, 753, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 265, 818
HpyF3I CTNAG 3 cut(s) 101, 642, 677
Hsp92II CATG 4 cut(s) 95, 436, 628, 757
HspAI GCGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 115, 513, 645
LmnI GCTCC 2 cut(s) 29, 757
Lsp1109I GCAGC 4 cut(s) 212, 289, 376, 772
LweI GCATC 2 cut(s) 401, 830
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 117, 515, 647
MboI GATC 3 cut(s) 115, 513, 645
MboII GAAGA 7 cut(s) 42, 137, 327, 737, 887, 897, 928
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 3 cut(s) 270, 636, 982
MlsI TGGCCA 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 2 cut(s) 445, 449
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 6 cut(s) 43, 388, 584, 654, 657, 682
Mox20I TGGCCA 1 cut(s) 248
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 248
MseI TTAA 3 cut(s) 123, 318, 459
MslI CAYNNNNRTG 2 cut(s) 546, 629
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 774
MspI CCGG 1 cut(s) 792
MwoI GCNNNNNNNGC 2 cut(s) 265, 818
NdeII GATC 3 cut(s) 115, 513, 645
NlaIII CATG 4 cut(s) 95, 436, 628, 757
NlaIV GGNNCC 3 cut(s) 31, 352, 946
NsiI ATGCAT 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 546
PaeR7I CTCGAG 1 cut(s) 34
PfeI GAWTC 3 cut(s) 363, 407, 927
PkrI GCNGC 6 cut(s) 11, 227, 304, 391, 762, 776
PleI GAGTC 2 cut(s) 445, 448
PpsI GAGTC 2 cut(s) 445, 448
PspN4I GGNNCC 3 cut(s) 31, 352, 946
PspXI VCTCGAGB 1 cut(s) 34
PstI CTGCAG 2 cut(s) 14, 307
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 513
RsaI GTAC 3 cut(s) 668, 736, 935
RsaNI GTAC 3 cut(s) 667, 735, 934
RseI CAYNNNNRTG 2 cut(s) 546, 629
SaqAI TTAA 3 cut(s) 123, 318, 459
SatI GCNGC 6 cut(s) 10, 226, 303, 390, 761, 775
Sau3AI GATC 3 cut(s) 115, 513, 645
SchI GAGTC 2 cut(s) 445, 449
SduI GDGCHC 3 cut(s) 270, 636, 982
SetI ASST 7 cut(s) 41, 45, 304, 391, 558, 668, 915
SfaNI GCATC 2 cut(s) 401, 830
SfcI CTRYAG 2 cut(s) 10, 303
Sfr274I CTCGAG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 546, 629
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SsiI CCGC 1 cut(s) 774
StyI CCWWGG 3 cut(s) 43, 270, 948
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 1 cut(s) 35
TatI WGTACW 1 cut(s) 933
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 3 cut(s) 363, 407, 927
Tru1I TTAA 3 cut(s) 123, 318, 459
Tru9I TTAA 3 cut(s) 123, 318, 459
TscAI CASTG 2 cut(s) 105, 833
TseI GCWGC 5 cut(s) 9, 225, 302, 389, 760
TspDTI ATGAA 3 cut(s) 355, 421, 641
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 2 cut(s) 105, 833
XapI RAATTY 2 cut(s) 891, 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XhoI CTCGAG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 500
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.