Rw7G012740

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001687
Physical Location & Seq
Reverse (-)
12097346 .. 12100576
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012740.1

Sequence Viewer

Length: 891 bp
ATGGAGAAGAACAAAGTCTCAAGAGTTTGTGTTACAGGTGGTTCTGGATATATTGGCTGTTGGCTAGTAAAAACGCTCCTAGAGAAGGGCCACACAGTCCATGCCACTCTCAGAAACTTGGAGGATGCATCAAAAGTAGGCCTTCTGAAGTCTCTCCCCAATGCAGACACCAATTTGTTGCTGTTCAAAGCTGATATATACGATCCTAATGAATTCGAGCCTGCAATTGAAGGGTGTGAGTTTGTTTTTCATGTTGCAACTCCCATGCAACATGATCCCCAAAACTCCCAGTACAAAGACACAGCTGAAGCTGCTGTTGCCGGGGTGAGAAGCATTGCTGATTCTTGCATTCGATCGCAGACAGTTAAGCGACTCATCTACACTGCAAGCATACTTTCTATGTCGCCGAGAAAGGATGATGGAGTTGGATTCAGACCCTTACTTGATGAGTCATGTTGGACACCACTTGATGCCTCATCTACTTATGGCAATGCTATCACGATGGGGTACATTAAATCAAAGACCTTAGCTGAGAAAGCAGTGCTGAGCCACAATGACTTTGATGATGGTAAATTAGAAGTGGTAACTCTTCCTTGTGGCTTAGTGGGAGGAGAAAGTCTTCTTTCATATTTGCCTACAAGTGTGGGAGTGATTCTGGCACAACTTACTGGGGACTTGTTTTCTTGCAATGCCTTAAAATTAATGCAAGAATTTATGGGATCAGTTCCTCTGGTGCACATTGATGATGTTTGCCAAGCACATATCTTCTGCATGGAACAGCCATCAATGAAAGGCAGATTCTGTTGTGCAGTTGCCTGTCACTCTATCAAAGAAATTGCAATATACTTCCAAGAAACTTACCCAGAATACAAGATACAAAAAAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

296

Amino Acids

32.44

Weight (kDa)

5.77

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 109 1.6e-10 NmrA-like family
Epimerase PF01370 9 - 262 1.1e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 198 5.1e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 1.1e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 210 4.5e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 132 2.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 197, 269, 727
AcsI RAATTY 2 cut(s) 212, 710
AcuI CTGAAG 2 cut(s) 167, 327
AfaI GTAC 2 cut(s) 293, 509
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 2 cut(s) 187, 230
AluBI AGCT 5 cut(s) 191, 305, 311, 530, 887
AluI AGCT 5 cut(s) 191, 305, 311, 530, 887
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 2 cut(s) 22, 156
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 3 cut(s) 197, 269, 727
AlwNI CAGNNNCTG 1 cut(s) 801
AoxI GGCC 2 cut(s) 88, 139
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 212, 710
AseI ATTAAT 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 1 cut(s) 298
BccI CCATC 4 cut(s) 413, 496, 560, 790
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 22, 156
BfaI CTAG 2 cut(s) 65, 80
BisI GCNGC 1 cut(s) 312
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 2 cut(s) 283, 678
BmsI GCATC 3 cut(s) 115, 137, 460
BmuI ACTGGG 2 cut(s) 283, 678
BpiI GAAGAC 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 4
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 289, 673
Bse3DI GCAATG 3 cut(s) 333, 496, 694
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 2 cut(s) 130, 421
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 3 cut(s) 333, 496, 694
BseMII CTCAG 3 cut(s) 124, 522, 536
BseNI ACTGG 2 cut(s) 289, 673
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 2 cut(s) 90, 141
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 2 cut(s) 22, 156
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 2 cut(s) 90, 141
Bsp1286I GDGCHC 1 cut(s) 738
Bsp143I GATC 4 cut(s) 202, 274, 353, 719
Bsp1720I GCTNAGC 1 cut(s) 545
BspANI GGCC 2 cut(s) 90, 141
BspCNI CTCAG 3 cut(s) 123, 523, 537
BspPI GGATC 3 cut(s) 197, 269, 727
BsrDI GCAATG 3 cut(s) 333, 496, 694
BsrI ACTGG 2 cut(s) 289, 673
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 202, 274, 353, 719
Bst4CI ACNGT 2 cut(s) 97, 364
Bst6I CTCTTC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 222, 388
BstDEI CTNAG 6 cut(s) 110, 526, 531, 545, 601, 888
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 2 cut(s) 130, 421
BstKTI GATC 4 cut(s) 205, 277, 356, 722
BstMAI GTCTC 2 cut(s) 22, 156
BstMBI GATC 4 cut(s) 202, 274, 353, 719
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 3 cut(s) 311, 317, 536
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 738
BstV1I GCAGC 1 cut(s) 298
BstV2I GAAGAC 1 cut(s) 611
BsuRI GGCC 2 cut(s) 90, 141
BtsCI GGATG 2 cut(s) 130, 421
BtsI GCAGTG 2 cut(s) 381, 546
BtsIMutI CAGTG 2 cut(s) 381, 546
Cac8I GCNNGC 2 cut(s) 222, 388
CaiI CAGNNNCTG 1 cut(s) 801
Cfr13I GGNCC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 292, 508
CviAII CATG 6 cut(s) 101, 251, 265, 272, 453, 772
CviQI GTAC 2 cut(s) 292, 508
DdeI CTNAG 6 cut(s) 110, 526, 531, 545, 601, 888
DpnI GATC 4 cut(s) 204, 276, 355, 721
DpnII GATC 4 cut(s) 202, 274, 353, 719
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco147I AGGCCT 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 167, 327
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 212
EcoT22I ATGCAT 1 cut(s) 130
FaeI CATG 6 cut(s) 104, 254, 268, 275, 456, 775
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 1 cut(s) 686
FatI CATG 6 cut(s) 100, 250, 264, 271, 452, 771
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 137, 428
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 65, 80
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 2 cut(s) 90, 141
HapII CCGG 1 cut(s) 321
Hin1II CATG 6 cut(s) 104, 254, 268, 275, 456, 775
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 6 cut(s) 341, 372, 429, 449, 652, 798
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 3 cut(s) 113, 147, 434
Hpy188III TCNNGA 3 cut(s) 21, 45, 499
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 3 cut(s) 79, 152, 224
HpyCH4III ACNGT 2 cut(s) 97, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 317, 536
HpyF3I CTNAG 6 cut(s) 110, 526, 531, 545, 601, 888
Hsp92II CATG 6 cut(s) 104, 254, 268, 275, 456, 775
Kzo9I GATC 4 cut(s) 202, 274, 353, 719
LmnI GCTCC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 3 cut(s) 115, 137, 460
MaeI CTAG 2 cut(s) 65, 80
MaeIII GTNAC 3 cut(s) 31, 583, 818
MalI GATC 4 cut(s) 204, 276, 355, 721
MboI GATC 4 cut(s) 202, 274, 353, 719
MboII GAAGA 4 cut(s) 19, 581, 611, 757
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 738
MluCI AATT 7 cut(s) 172, 212, 225, 572, 698, 710, 834
MlyI GAGTC 2 cut(s) 366, 458
MmeI TCCRAC 2 cut(s) 406, 437
MnlI CCTC 4 cut(s) 115, 484, 602, 738
Mph1103I ATGCAT 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 618
MseI TTAA 4 cut(s) 366, 513, 695, 701
MslI CAYNNNNRTG 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 311, 317, 536
NciI CCSGG 1 cut(s) 322
NdeII GATC 4 cut(s) 202, 274, 353, 719
NlaIII CATG 6 cut(s) 104, 254, 268, 275, 456, 775
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 130
PceI AGGCCT 1 cut(s) 141
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 341, 429, 652, 798
PkrI GCNGC 1 cut(s) 313
Ple19I CGATCG 1 cut(s) 356
PleI GAGTC 2 cut(s) 366, 457
PpsI GAGTC 2 cut(s) 366, 457
PshBI ATTAAT 1 cut(s) 701
PspPI GGNCC 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 801
PvuI CGATCG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 293, 509
RsaNI GTAC 2 cut(s) 292, 508
RseI CAYNNNNRTG 1 cut(s) 741
SaqAI TTAA 4 cut(s) 366, 513, 695, 701
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 202, 274, 353, 719
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 2 cut(s) 366, 458
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 1 cut(s) 738
SetI ASST 7 cut(s) 40, 193, 307, 313, 527, 532, 889
SfaNI GCATC 3 cut(s) 115, 137, 460
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 7 cut(s) 172, 212, 225, 572, 698, 710, 834
SseBI AGGCCT 1 cut(s) 141
SspMI CTAG 2 cut(s) 65, 80
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 2 cut(s) 97, 364
TaqI TCGA 2 cut(s) 216, 352
TasI AATT 7 cut(s) 172, 212, 225, 572, 698, 710, 834
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 4 cut(s) 341, 429, 652, 798
Tru1I TTAA 4 cut(s) 366, 513, 695, 701
Tru9I TTAA 4 cut(s) 366, 513, 695, 701
TscAI CASTG 2 cut(s) 388, 546
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 4 cut(s) 225, 239, 615, 803
TspRI CASTG 2 cut(s) 388, 546
VneI GTGCAC 1 cut(s) 734
VspI ATTAAT 1 cut(s) 701
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 2 cut(s) 212, 710
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 2 cut(s) 65, 80
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.