FvH4_7g16111

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001687
Physical Location & Seq
Forward (+)
13877414 .. 13880084
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16111.t2

Sequence Viewer

Length: 1074 bp
ATGAGATATATGATAAGGGACTGCGGAAACTTAAGATCTCAGAGAGTTTCGATGGAGAAGAGTAATGGCTCCAAGGTTTGTGTTACAGGAGGTGCAGGATTCATAGGCTCTTTGCTTGTCAAGAAGCTTTTAGAGAAGGGATACACTGTCCATGCAACACTCAGAAATTTGGATGATGTTTCGAAGGTGAGCCTTCTGAGGAACCTTCCATATTCTGATAAAAGGCTGATACTGTTTGAAGCTGACATCTACAATCCAGTTACATTTGAGCAGGCAATTGAAGGATGTGAGTTTGTTTTCCATGTAGCTACTCCCTTCAACCACACCCCAAATTCCCAGTACAAGAACAAAATTGAAGCAACTATAGCTGGGGCAAAGAGCATTGCAGAGGCTTGTCTTAAATCAGGAGCAGTTAGGAGGCTTATCTACACCAGTTCCGTGGTTGCTGCATCTCCACTTAAAGAAGATACTAGTGGTTTCAAAGACATCATGGATGAAACATGTTGGACTCCTCTTGATAATCTCCCAGTTTTTTGTTCTAATCACTTGCTCAAGGACTATACAGATTCAAAGACACTGGCAGAGAAAGAGATCTTGAGTTATGGGAATCACAACAAAAGAAGTGGACTTGAGATTGTGAGCTTGACCTGTGGTCTTGTGGGAGGGAATTCACTTCTACAATTCACACCTGAAACTGTAACTTGGTTAGTTTCTTATTTTAAGAGTGGTGCAATGGAGTATAGTTGTATGACATTCTTAGAGGAATTGGTAGGGAAAATTCCAATAGTCCATATTGATGATGTTTGTGATGCCCATATCTTCTGCATGGAGAGTCCTTCAATAAATGGTAGATTCCTGTGCGCAAGATCCTATGTTTCATCTGCACACATTGCTAGCTACTATCGGCAACATTATCCAGAACTTGAAGTGAAGCAAGAGTGTTTGGATTTACCAAATAGGCAAATTGGCTGGGGTGGTACCAAGCTTACTGAGGAGGGTTTTGTGTATAAATATGATACCAATATGATTTTGGATGATTGCATAACTTGTGCAAGAAAATGGGGTACCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

358

Amino Acids

39.94

Weight (kDa)

6.32

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 24 - 148 1.4e-10 NmrA-like family
Epimerase PF01370 26 - 279 2.4e-21 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 26 - 153 1.3e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 28 - 243 2.3e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 28 - 271 4.8e-12 Male sterility protein
GDP_Man_Dehyd PF16363 28 - 145 4.6e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 30 - 155 6.1e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 862
Acc65I GGTACC 2 cut(s) 977, 1064
AccB1I GGYRCC 2 cut(s) 977, 1064
AciI CCGC 1 cut(s) 24
AclWI GGATC 1 cut(s) 861
AcsI RAATTY 4 cut(s) 166, 331, 667, 777
AfaI GTAC 3 cut(s) 341, 979, 1066
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 500
AgsI TTSAA 8 cut(s) 239, 281, 319, 356, 481, 570, 840, 926
AhdI GACNNNNNGTC 1 cut(s) 651
AhlI ACTAGT 1 cut(s) 470
AluBI AGCT 7 cut(s) 127, 242, 308, 368, 642, 897, 985
AluI AGCT 7 cut(s) 127, 242, 308, 368, 642, 897, 985
AlwI GGATC 1 cut(s) 861
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 4 cut(s) 166, 331, 667, 777
Asp718I GGTACC 2 cut(s) 977, 1064
AspLEI GCGC 1 cut(s) 863
AsuHPI GGTGA 1 cut(s) 199
AsuII TTCGAA 1 cut(s) 182
AsuNHI GCTAGC 1 cut(s) 893
BanI GGYRCC 2 cut(s) 977, 1064
BbvI GCAGC 1 cut(s) 433
BccI CCATC 1 cut(s) 46
BciVI GTATCC 1 cut(s) 134
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 3 cut(s) 471, 894, 1072
BfmI CTRYAG 1 cut(s) 363
BfrI CTTAAG 1 cut(s) 31
BfuI GTATCC 1 cut(s) 134
BglII AGATCT 2 cut(s) 35, 591
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
BmeRI GACNNNNNGTC 1 cut(s) 651
BmiI GGNNCC 4 cut(s) 70, 203, 979, 1066
BmrI ACTGGG 2 cut(s) 331, 521
BmsI GCATC 2 cut(s) 458, 799
BmtI GCTAGC 1 cut(s) 897
BmuI ACTGGG 2 cut(s) 331, 521
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 3 cut(s) 536, 616, 650
BsaJI CCNNGG 2 cut(s) 72, 438
Bse1I ACTGG 5 cut(s) 257, 337, 432, 527, 582
Bse3DI GCAATG 3 cut(s) 381, 738, 888
BseDI CCNNGG 2 cut(s) 72, 438
BseGI GGATG 4 cut(s) 178, 290, 499, 1039
BseMI GCAATG 3 cut(s) 381, 738, 888
BseMII CTCAG 4 cut(s) 53, 175, 188, 981
BseNI ACTGG 5 cut(s) 257, 337, 432, 527, 582
BseRI GAGGAG 2 cut(s) 501, 1007
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 2 cut(s) 368, 969
BsgI GTGCAG 2 cut(s) 114, 867
BshNI GGYRCC 2 cut(s) 977, 1064
BslFI GGGAC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 32
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 3 cut(s) 35, 591, 866
BspACI CCGC 1 cut(s) 24
BspCNI CTCAG 4 cut(s) 52, 174, 189, 982
BspLI GGNNCC 4 cut(s) 70, 203, 979, 1066
BspOI GCTAGC 1 cut(s) 897
BspPI GGATC 1 cut(s) 861
BspT104I TTCGAA 1 cut(s) 182
BspT107I GGYRCC 2 cut(s) 977, 1064
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 381, 738, 888
BsrI ACTGG 5 cut(s) 257, 337, 432, 527, 582
BssECI CCNNGG 2 cut(s) 72, 438
BssMI GATC 3 cut(s) 35, 591, 866
BssT1I CCWWGG 1 cut(s) 72
Bst4CI ACNGT 3 cut(s) 148, 234, 697
Bst6I CTCTTC 1 cut(s) 53
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 890
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 273, 895
BstDEI CTNAG 5 cut(s) 39, 161, 197, 757, 990
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 4 cut(s) 178, 290, 499, 1039
BstHHI GCGC 1 cut(s) 863
BstKTI GATC 3 cut(s) 38, 594, 869
BstMBI GATC 3 cut(s) 35, 591, 866
BstMWI GCNNNNNNNGC 2 cut(s) 365, 890
BstNSI RCATGY 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 433
BstX2I RGATCY 3 cut(s) 35, 591, 866
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 3 cut(s) 35, 591, 866
BsuI GTATCC 1 cut(s) 134
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 4 cut(s) 178, 290, 499, 1039
BtsIMutI CAGTG 2 cut(s) 144, 575
Cac8I GCNNGC 2 cut(s) 273, 895
CfoI GCGC 1 cut(s) 863
Csp6I GTAC 3 cut(s) 340, 978, 1065
CspCI CAANNNNNGTGG 2 cut(s) 604, 639
CviAII CATG 5 cut(s) 152, 302, 490, 501, 826
CviQI GTAC 3 cut(s) 340, 978, 1065
DdeI CTNAG 5 cut(s) 39, 161, 197, 757, 990
DpnI GATC 3 cut(s) 37, 593, 868
DpnII GATC 3 cut(s) 35, 591, 866
DriI GACNNNNNGTC 1 cut(s) 651
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 5 cut(s) 155, 305, 493, 504, 829
FaqI GGGAC 1 cut(s) 32
FatI CATG 5 cut(s) 151, 301, 489, 500, 825
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 4 cut(s) 185, 297, 506, 1046
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 3 cut(s) 471, 894, 1072
FspI TGCGCA 1 cut(s) 862
GlaI GCGC 1 cut(s) 862
GluI GCNGC 1 cut(s) 447
GsaI CCCAGC 2 cut(s) 372, 973
HhaI GCGC 1 cut(s) 863
Hin1II CATG 5 cut(s) 155, 305, 493, 504, 829
Hin6I GCGC 1 cut(s) 861
HinP1I GCGC 1 cut(s) 861
HindIII AAGCTT 2 cut(s) 125, 983
HinfI GANTC 6 cut(s) 99, 508, 566, 607, 832, 852
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 626
Hpy188I TCNGA 4 cut(s) 42, 164, 198, 217
Hpy188III TCNNGA 5 cut(s) 121, 405, 515, 595, 917
Hpy8I GTNNAC 1 cut(s) 626
HpyAV CCTTC 7 cut(s) 130, 178, 203, 215, 275, 325, 846
HpyCH4III ACNGT 3 cut(s) 148, 234, 697
HpyCH4V TGCA 9 cut(s) 95, 155, 386, 449, 731, 825, 884, 1041, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 890
HpyF3I CTNAG 5 cut(s) 39, 161, 197, 757, 990
Hsp92II CATG 5 cut(s) 155, 305, 493, 504, 829
HspAI GCGC 1 cut(s) 861
KpnI GGTACC 2 cut(s) 981, 1068
Kzo9I GATC 3 cut(s) 35, 591, 866
LmnI GCTCC 2 cut(s) 74, 407
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 458, 799
MaeI CTAG 3 cut(s) 471, 894, 1072
MaeIII GTNAC 3 cut(s) 82, 259, 697
MalI GATC 3 cut(s) 37, 593, 868
MboI GATC 3 cut(s) 35, 591, 866
MboII GAAGA 3 cut(s) 70, 476, 811
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 3 cut(s) 35, 591, 866
MluCI AATT 9 cut(s) 166, 276, 331, 351, 667, 680, 764, 777, 963
MlyI GAGTC 2 cut(s) 502, 841
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 9 cut(s) 83, 192, 382, 411, 522, 656, 754, 985, 988
MseI TTAA 4 cut(s) 32, 399, 459, 720
MslI CAYNNNNRTG 1 cut(s) 795
MspCI CTTAAG 1 cut(s) 31
MunI CAATTG 1 cut(s) 276
MwoI GCNNNNNNNGC 2 cut(s) 365, 890
NdeII GATC 3 cut(s) 35, 591, 866
NheI GCTAGC 1 cut(s) 893
NlaIII CATG 5 cut(s) 155, 305, 493, 504, 829
NlaIV GGNNCC 4 cut(s) 70, 203, 979, 1066
NsbI TGCGCA 1 cut(s) 862
NspI RCATGY 1 cut(s) 504
NspV TTCGAA 1 cut(s) 182
PciI ACATGT 1 cut(s) 500
PfeI GAWTC 4 cut(s) 99, 566, 607, 852
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 2 cut(s) 502, 840
PpsI GAGTC 2 cut(s) 502, 840
PscI ACATGT 1 cut(s) 500
PspFI CCCAGC 2 cut(s) 368, 969
PspN4I GGNNCC 4 cut(s) 70, 203, 979, 1066
PsuI RGATCY 3 cut(s) 35, 591, 866
RsaI GTAC 3 cut(s) 341, 979, 1066
RsaNI GTAC 3 cut(s) 340, 978, 1065
RseI CAYNNNNRTG 1 cut(s) 795
SaqAI TTAA 4 cut(s) 32, 399, 459, 720
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 3 cut(s) 35, 591, 866
SchI GAGTC 2 cut(s) 502, 841
SfaNI GCATC 2 cut(s) 458, 799
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 4 cut(s) 31, 551, 595, 629
SmoI CTYRAG 4 cut(s) 31, 551, 595, 629
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 9 cut(s) 166, 276, 331, 351, 667, 680, 764, 777, 963
SsiI CCGC 1 cut(s) 24
SspMI CTAG 3 cut(s) 471, 894, 1072
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 3 cut(s) 148, 234, 697
TaqI TCGA 2 cut(s) 50, 182
TasI AATT 9 cut(s) 166, 276, 331, 351, 667, 680, 764, 777, 963
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 4 cut(s) 99, 566, 607, 852
Tru1I TTAA 4 cut(s) 32, 399, 459, 720
Tru9I TTAA 4 cut(s) 32, 399, 459, 720
TscAI CASTG 2 cut(s) 151, 582
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 3 cut(s) 91, 510, 867
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 2 cut(s) 151, 582
Vha464I CTTAAG 1 cut(s) 31
XapI RAATTY 4 cut(s) 166, 331, 667, 777
XceI RCATGY 1 cut(s) 504
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XspI CTAG 3 cut(s) 471, 894, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.