Rh1BG248100

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001687
Physical Location & Seq
Forward (+)
37595238 .. 37596947
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG248100.1

Sequence Viewer

Length: 1020 bp
ATGGCGAAGAGTAATGGCTGCAAGGTTTGTATTACAGGAGGTGCAGGGTACATAGGCTCTTGGCTTGTCAAGAAGCTTTTAGAGAAGGGATACACTGTCCATGCAACACTCAGAAACTTGGATGATGTTTCGAAGGTGAACCTTCTGAAGAGCCTTCCTGATTCTGATGAAAGGCTAGTGCTTTTTGAAGCTGACTTCTATAATCCAGTTACGTTTGAGCAGGCAATTGAAGGATGTGAATTTGTTTTCCATGTAGCAACTCCCTTGCTTCACACCCCAAACTCCCAGTACAAGAACACAACTGAAGCTGCTATAGCTGGGGCAAAGAGCATCGTAGAGGCTTGTCTTAAATCAGGAACAGTTAGGAGGCTAATCTACACTGCTTCCGTGGTTGCTGCATCTCCCCTTAAAGAAGATGGTAGTGGTTTCAAAGACTTCATGGATGAAACATGTTGGACTCCTCTAGATCTCACAGTTGCTTGTTCTAATGACTTCCTCAAGGACTATGAAGATTCAAAGACACTGGCAGAGAAAGAAATCTTGAGCTATGTGAATCACGACAAAAGAAGTGGACTTGAGATTGTGAGCTTGGCCTGTGGTCTTGTGGGAGGGGACTCACTTCCATCATTCACGCCTGTAACTGTAGCTTGTTTAGTTTCTCAGCTTACAAATAGTGCAAATGAGTACAATTGTCTGAGATTCTTAGAGGAATTGCTAGGGAAAATTCCAATAGTCCATATTGATGATGTTTGTGATGCTCATATCTTCTGCATGGAGAGTCCTTCAATAAATGGTAGGTTTCTGTGCACAAGATCCTATGTTTCATCTGCACAAATTGCTAGTAACTATCAGCAACATTATCCAGAACTTGAAGTGAAGCAAGAGTGTTTGGATTTACCAAAGAGGCAAATTGGCTGGGGTTCTACCAAGCTTAGTGAGGAGGGATTTGTGTACAAATATGATACCAAGATGATATTGGATGATTGCATAGCGTCTGCAAGAAGATGGGGTAGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

339

Amino Acids

37.45

Weight (kDa)

5.19

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 128 7.2e-11 NmrA-like family
Epimerase PF01370 9 - 261 6.3e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 191 1.9e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 128 1.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 251 5.9e-10 Male sterility protein
NAD_binding_10 PF13460 13 - 134 4.2e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 807
AcsI RAATTY 2 cut(s) 239, 723
AcuI CTGAAG 2 cut(s) 167, 324
AfaI GTAC 4 cut(s) 50, 290, 686, 953
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 6 cut(s) 188, 230, 430, 516, 786, 872
AluBI AGCT 9 cut(s) 76, 191, 308, 317, 546, 588, 647, 664, 931
AluI AGCT 9 cut(s) 76, 191, 308, 317, 546, 588, 647, 664, 931
Alw21I GWGCWC 1 cut(s) 809
Alw44I GTGCAC 1 cut(s) 805
AlwI GGATC 1 cut(s) 807
AoxI GGCC 1 cut(s) 591
ApaLI GTGCAC 1 cut(s) 805
ApeKI GCWGC 3 cut(s) 18, 308, 395
ApoI RAATTY 2 cut(s) 239, 723
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 809
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 3 cut(s) 5, 295, 382
BccI CCATC 3 cut(s) 410, 631, 999
BciVI GTATCC 1 cut(s) 83
BfaI CTAG 4 cut(s) 176, 464, 716, 840
BfmI CTRYAG 2 cut(s) 312, 642
BfuI GTATCC 1 cut(s) 83
BglII AGATCT 1 cut(s) 466
BisI GCNGC 3 cut(s) 19, 309, 396
BlsI GCNGC 3 cut(s) 20, 310, 397
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 3 cut(s) 339, 407, 745
BmuI ACTGGG 1 cut(s) 280
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 3 cut(s) 482, 562, 596
BsaJI CCNNGG 1 cut(s) 387
Bse1I ACTGG 3 cut(s) 206, 286, 528
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 4 cut(s) 127, 239, 448, 985
BseMII CTCAG 3 cut(s) 124, 674, 686
BseNI ACTGG 3 cut(s) 206, 286, 528
BseRI GAGGAG 2 cut(s) 450, 953
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 3 cut(s) 5, 295, 382
BseYI CCCAGC 2 cut(s) 317, 915
BsgI GTGCAG 2 cut(s) 63, 813
BshFI GGCC 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 809
BslFI GGGAC 1 cut(s) 626
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 809
Bsp1407I TGTACA 1 cut(s) 951
Bsp143I GATC 2 cut(s) 466, 812
BspANI GGCC 1 cut(s) 593
BspCNI CTCAG 3 cut(s) 123, 673, 687
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 951
BsrI ACTGG 3 cut(s) 206, 286, 528
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 466, 812
Bst4CI ACNGT 4 cut(s) 97, 361, 475, 643
Bst6I CTCTTC 2 cut(s) 2, 143
BstAPI GCANNNNNTGC 1 cut(s) 836
BstAUI TGTACA 1 cut(s) 951
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 5 cut(s) 110, 660, 695, 703, 932
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 127, 239, 448, 985
BstKTI GATC 2 cut(s) 469, 815
BstMBI GATC 2 cut(s) 466, 812
BstMWI GCNNNNNNNGC 2 cut(s) 314, 836
BstNSI RCATGY 1 cut(s) 453
BstSFI CTRYAG 2 cut(s) 312, 642
BstSLI GKGCMC 1 cut(s) 809
BstV1I GCAGC 3 cut(s) 5, 295, 382
BstX2I RGATCY 2 cut(s) 466, 812
BstYI RGATCY 2 cut(s) 466, 812
BsuI GTATCC 1 cut(s) 83
BsuRI GGCC 1 cut(s) 593
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 4 cut(s) 127, 239, 448, 985
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 93, 378, 521
Cac8I GCNNGC 1 cut(s) 222
CseI GACGC 1 cut(s) 981
Csp6I GTAC 4 cut(s) 49, 289, 685, 952
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 5 cut(s) 101, 251, 439, 450, 772
CviQI GTAC 4 cut(s) 49, 289, 685, 952
DdeI CTNAG 5 cut(s) 110, 660, 695, 703, 932
DpnI GATC 2 cut(s) 468, 814
DpnII GATC 2 cut(s) 466, 812
Eam1104I CTCTTC 2 cut(s) 2, 143
EarI CTCTTC 2 cut(s) 2, 143
Eco57I CTGAAG 2 cut(s) 167, 324
FaeI CATG 5 cut(s) 104, 254, 442, 453, 775
FaqI GGGAC 1 cut(s) 626
FatI CATG 5 cut(s) 100, 250, 438, 449, 771
Fnu4HI GCNGC 3 cut(s) 19, 309, 396
FokI GGATG 4 cut(s) 134, 246, 455, 992
Fsp4HI GCNGC 3 cut(s) 19, 309, 396
FspBI CTAG 4 cut(s) 176, 464, 716, 840
GluI GCNGC 3 cut(s) 19, 309, 396
GsaI CCCAGC 2 cut(s) 321, 919
HaeIII GGCC 1 cut(s) 593
HgaI GACGC 1 cut(s) 981
Hin1II CATG 5 cut(s) 104, 254, 442, 453, 775
HindIII AAGCTT 2 cut(s) 74, 929
HinfI GANTC 7 cut(s) 161, 457, 512, 553, 614, 699, 778
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 4 cut(s) 139, 572, 807, 952
Hpy188I TCNGA 4 cut(s) 113, 147, 166, 696
Hpy188III TCNNGA 7 cut(s) 70, 158, 354, 464, 541, 557, 863
Hpy8I GTNNAC 4 cut(s) 139, 572, 807, 952
HpyAV CCTTC 6 cut(s) 79, 127, 152, 164, 224, 792
HpyCH4III ACNGT 4 cut(s) 97, 361, 475, 643
HpyCH4IV ACGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 836
HpyF3I CTNAG 5 cut(s) 110, 660, 695, 703, 932
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 5 cut(s) 104, 254, 442, 453, 775
Kzo9I GATC 2 cut(s) 466, 812
LguI GCTCTTC 1 cut(s) 143
Lsp1109I GCAGC 3 cut(s) 5, 295, 382
LweI GCATC 3 cut(s) 339, 407, 745
MaeI CTAG 4 cut(s) 176, 464, 716, 840
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 208, 637, 842
MalI GATC 2 cut(s) 468, 814
MboI GATC 2 cut(s) 466, 812
MboII GAAGA 6 cut(s) 19, 160, 425, 521, 757, 1014
MfeI CAATTG 2 cut(s) 225, 688
MflI RGATCY 2 cut(s) 466, 812
MhlI GDGCHC 1 cut(s) 809
MluCI AATT 7 cut(s) 225, 239, 688, 710, 723, 834, 909
MlyI GAGTC 3 cut(s) 451, 608, 787
MmeI TCCRAC 1 cut(s) 434
MseI TTAA 2 cut(s) 348, 408
MslI CAYNNNNRTG 1 cut(s) 741
MunI CAATTG 2 cut(s) 225, 688
MwoI GCNNNNNNNGC 2 cut(s) 314, 836
NdeII GATC 2 cut(s) 466, 812
NlaIII CATG 5 cut(s) 104, 254, 442, 453, 775
NspI RCATGY 1 cut(s) 453
NspV TTCGAA 1 cut(s) 131
PciI ACATGT 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 143
PfeI GAWTC 4 cut(s) 161, 512, 553, 699
PkrI GCNGC 3 cut(s) 20, 310, 397
PleI GAGTC 3 cut(s) 451, 608, 786
PpsI GAGTC 3 cut(s) 451, 608, 786
PscI ACATGT 1 cut(s) 449
PspFI CCCAGC 2 cut(s) 317, 915
PsuI RGATCY 2 cut(s) 466, 812
RsaI GTAC 4 cut(s) 50, 290, 686, 953
RsaNI GTAC 4 cut(s) 49, 289, 685, 952
RseI CAYNNNNRTG 1 cut(s) 741
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 348, 408
SatI GCNGC 3 cut(s) 19, 309, 396
Sau3AI GATC 2 cut(s) 466, 812
SchI GAGTC 3 cut(s) 451, 608, 787
SduI GDGCHC 1 cut(s) 809
SfaNI GCATC 3 cut(s) 339, 407, 745
SfcI CTRYAG 2 cut(s) 312, 642
SfuI TTCGAA 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 3 cut(s) 497, 541, 575
SmoI CTYRAG 3 cut(s) 497, 541, 575
Sse9I AATT 7 cut(s) 225, 239, 688, 710, 723, 834, 909
SspMI CTAG 4 cut(s) 176, 464, 716, 840
TaaI ACNGT 4 cut(s) 97, 361, 475, 643
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 131
TasI AATT 7 cut(s) 225, 239, 688, 710, 723, 834, 909
TatI WGTACW 3 cut(s) 288, 684, 951
TfiI GAWTC 4 cut(s) 161, 512, 553, 699
Tru1I TTAA 2 cut(s) 348, 408
Tru9I TTAA 2 cut(s) 348, 408
TscAI CASTG 3 cut(s) 100, 385, 528
TseI GCWGC 3 cut(s) 18, 308, 395
TspDTI ATGAA 5 cut(s) 183, 427, 459, 522, 813
TspGWI ACGGA 1 cut(s) 376
TspRI CASTG 3 cut(s) 100, 385, 528
VneI GTGCAC 1 cut(s) 805
XapI RAATTY 2 cut(s) 239, 723
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XspI CTAG 4 cut(s) 176, 464, 716, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.