MD01G1077400.v1.1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001687
Physical Location & Seq
Forward (+)
18412910 .. 18415367
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1077400.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGAGCATGAGTACTAGCTACAAGGTATGTGTTATAGGCGGTGCAGGCTACATTGGTTCTTGGCTTGTCAAGCAGCTTTTAGAAAAAGGATACACTGTCCATGCAACACTCCGCAACTGGGATGATGTGTCCAAAGTGAGCCTTCTGAAGAGCCTTCCTGATGCTGATGATAGACTAGTGCTGTTTGAAGCTGACATTTACAACCCAATTGAGTTTGAGCATGCAATTGAAGGCTGTGAGTTTGTTTTCCATGTAGCAACTCCCTTACAACACACTGAAAATTCCCAGTACAAGAACATAACTGAGGCTGCCATAGCTGGGGCAAAGAGCATTGCAGAATGTTGTCTTAAATCAGGAACAGTTAAGAGGCTAATCTACACAGCATCCGTGGTGGCTGCATCTCCACTGAAAGAGGATGGGAGTGGATTTGGAAACTTGGTGGATGAAACCTGTTGGACTCCTCTCAGTCTTTCATTTGCTGGTTCTAATGGTCATCGTAAGGCCTATGTGGATTCAAAAACACTTGCAGAGAGAGAAATCTTGAGCTATGGGAACCAAAAAAATGGTGGACTGGAGGTTGTGACTCTGGCCTGTGGCCTTGTTGGGGGTGATGCACTTCTGCCTTTCACATCCTCGAGTGTGGCTGTTTTTATTTCTCAGCTTACAAACAGTGCAACCGAGTACCAATTACTTAGATTTCTGGAGGAATTGGTTGGGAAAATTCCTATAGTACACATCGATGATGTGTGTGATGCCCATATCTTCTGCATGGAAAAACCTTCAATAAGTGGTAGATTTTTGTGCGCAAGTTCATATGTTTCATCAGCAGAAATTGCTACTTACTACCAACAAAACTACCCAATATTTCAAGTGAAGCCAGAGTATTTGGATGGACCAAAAAGAGAAATTGTCTGGGGCTCTACAAAGCTTGAAGAGAAGGGTTTTGAATACAAGTACTGCACCAAGATGATATTAGATGATTGCATAAGGTGTGCAAGGAATAGAGGTGGCACTGACTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

342

Amino Acids

37.57

Weight (kDa)

5.37

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 128 5.7e-10 NmrA-like family
Epimerase PF01370 9 - 260 8.2e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 197 6.9e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 120 7e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 13 - 252 1.9e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 132 1.2e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 805
AciI CCGC 2 cut(s) 39, 112
AcsI RAATTY 2 cut(s) 280, 720
AcuI CTGAAG 1 cut(s) 167
AfaI GTAC 5 cut(s) 13, 290, 683, 732, 956
AfiI CCNNNNNNNGG 3 cut(s) 118, 318, 604
AgsI TTSAA 7 cut(s) 188, 230, 516, 783, 869, 932, 947
AhlI ACTAGT 1 cut(s) 175
AluBI AGCT 7 cut(s) 18, 76, 191, 317, 546, 661, 928
AluI AGCT 7 cut(s) 18, 76, 191, 317, 546, 661, 928
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 3 cut(s) 501, 588, 595
ApeKI GCWGC 3 cut(s) 73, 308, 395
ApoI RAATTY 2 cut(s) 280, 720
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 1 cut(s) 620
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 1 cut(s) 893
BanII GRGCYC 1 cut(s) 920
BbvI GCAGC 3 cut(s) 85, 295, 382
BccI CCATC 2 cut(s) 410, 884
BciVI GTATCC 1 cut(s) 83
BcuI ACTAGT 1 cut(s) 175
BfaI CTAG 2 cut(s) 15, 176
BfmI CTRYAG 1 cut(s) 726
BfuI GTATCC 1 cut(s) 83
BisI GCNGC 3 cut(s) 74, 309, 396
BlsI GCNGC 3 cut(s) 75, 310, 397
BmcAI AGTACT 2 cut(s) 13, 956
Bme18I GGWCC 1 cut(s) 893
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 1 cut(s) 554
BmrI ACTGGG 2 cut(s) 127, 280
BmsI GCATC 5 cut(s) 151, 392, 407, 601, 742
BmuI ACTGGG 2 cut(s) 127, 280
BpmI CTGGAG 2 cut(s) 593, 722
BpuEI CTTGAG 1 cut(s) 562
Bsa29I ATCGAT 1 cut(s) 738
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 318, 604
Bse1I ACTGG 3 cut(s) 122, 286, 576
Bse3DI GCAATG 1 cut(s) 330
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 6 cut(s) 127, 383, 421, 448, 629, 895
BseLI CCNNNNNNNGG 3 cut(s) 118, 318, 604
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 3 cut(s) 294, 478, 671
BseNI ACTGG 3 cut(s) 122, 286, 576
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 3 cut(s) 85, 295, 382
BseYI CCCAGC 1 cut(s) 317
BsgI GTGCAG 2 cut(s) 63, 943
BshFI GGCC 3 cut(s) 503, 590, 597
BshVI ATCGAT 1 cut(s) 738
BsiHKCI CYCGRG 1 cut(s) 634
BslI CCNNNNNNNGG 3 cut(s) 118, 318, 604
BsnI GGCC 3 cut(s) 503, 590, 597
BsoBI CYCGRG 1 cut(s) 634
Bsp1286I GDGCHC 1 cut(s) 920
BspACI CCGC 2 cut(s) 39, 112
BspANI GGCC 3 cut(s) 503, 590, 597
BspCNI CTCAG 3 cut(s) 295, 477, 670
BspDI ATCGAT 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 554
BspQI GCTCTTC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 3 cut(s) 122, 286, 576
BssECI CCNNGG 1 cut(s) 387
Bst4CI ACNGT 3 cut(s) 97, 361, 671
Bst6I CTCTTC 2 cut(s) 143, 927
BstAPI GCANNNNNTGC 1 cut(s) 833
BstC8I GCNNGC 2 cut(s) 46, 222
BstDEI CTNAG 4 cut(s) 303, 464, 657, 692
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 6 cut(s) 127, 383, 421, 448, 629, 895
BstHHI GCGC 1 cut(s) 806
BstMWI GCNNNNNNNGC 4 cut(s) 45, 70, 314, 833
BstNSI RCATGY 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 3 cut(s) 85, 295, 382
BstXI CCANNNNNNTGG 1 cut(s) 563
Bsu15I ATCGAT 1 cut(s) 738
BsuI GTATCC 1 cut(s) 83
BsuRI GGCC 3 cut(s) 503, 590, 597
BsuTUI ATCGAT 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 6 cut(s) 127, 383, 421, 448, 629, 895
BtsIMutI CAGTG 5 cut(s) 93, 273, 404, 676, 1011
Cac8I GCNNGC 2 cut(s) 46, 222
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 1 cut(s) 893
ClaI ATCGAT 1 cut(s) 738
Csp6I GTAC 5 cut(s) 12, 289, 682, 731, 955
CviAII CATG 5 cut(s) 7, 101, 221, 251, 769
CviQI GTAC 5 cut(s) 12, 289, 682, 731, 955
DdeI CTNAG 4 cut(s) 303, 464, 657, 692
Eam1104I CTCTTC 2 cut(s) 143, 927
EarI CTCTTC 2 cut(s) 143, 927
Eco147I AGGCCT 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 920
Eco47I GGWCC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 920
FaeI CATG 5 cut(s) 10, 104, 224, 254, 772
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FatI CATG 5 cut(s) 6, 100, 220, 250, 768
FauNDI CATATG 1 cut(s) 814
Fnu4HI GCNGC 3 cut(s) 74, 309, 396
FokI GGATG 6 cut(s) 134, 370, 428, 455, 616, 902
FriOI GRGCYC 1 cut(s) 920
Fsp4HI GCNGC 3 cut(s) 74, 309, 396
FspBI CTAG 2 cut(s) 15, 176
FspI TGCGCA 1 cut(s) 805
GlaI GCGC 1 cut(s) 805
GluI GCNGC 3 cut(s) 74, 309, 396
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 2 cut(s) 593, 722
HaeIII GGCC 3 cut(s) 503, 590, 597
HhaI GCGC 1 cut(s) 806
Hin1II CATG 5 cut(s) 10, 104, 224, 254, 772
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 3 cut(s) 457, 512, 583
HphI GGTGA 1 cut(s) 620
Hpy166II GTNNAC 2 cut(s) 569, 733
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 4 cut(s) 158, 354, 541, 701
Hpy8I GTNNAC 2 cut(s) 569, 733
HpyAV CCTTC 5 cut(s) 152, 164, 224, 789, 931
HpyCH4III ACNGT 3 cut(s) 97, 361, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 70, 314, 833
HpyF3I CTNAG 4 cut(s) 303, 464, 657, 692
Hsp92II CATG 5 cut(s) 10, 104, 224, 254, 772
HspAI GCGC 1 cut(s) 804
LguI GCTCTTC 1 cut(s) 143
Lsp1109I GCAGC 3 cut(s) 85, 295, 382
LweI GCATC 5 cut(s) 151, 392, 407, 601, 742
MaeI CTAG 2 cut(s) 15, 176
MaeIII GTNAC 1 cut(s) 580
MboII GAAGA 3 cut(s) 160, 754, 944
MfeI CAATTG 2 cut(s) 207, 225
MhlI GDGCHC 1 cut(s) 920
MluCI AATT 8 cut(s) 207, 225, 280, 686, 707, 720, 831, 906
MlyI GAGTC 2 cut(s) 451, 577
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 8 cut(s) 298, 360, 406, 471, 568, 643, 697, 998
MseI TTAA 2 cut(s) 348, 363
MslI CAYNNNNRTG 2 cut(s) 738, 965
MunI CAATTG 2 cut(s) 207, 225
MwoI GCNNNNNNNGC 4 cut(s) 45, 70, 314, 833
NdeI CATATG 1 cut(s) 814
NlaIII CATG 5 cut(s) 10, 104, 224, 254, 772
NlaIV GGNNCC 1 cut(s) 554
NmuCI GTSAC 1 cut(s) 580
NsbI TGCGCA 1 cut(s) 805
NspI RCATGY 1 cut(s) 224
PaeI GCATGC 1 cut(s) 224
PaeR7I CTCGAG 1 cut(s) 634
PceI AGGCCT 1 cut(s) 503
PciSI GCTCTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 3 cut(s) 75, 310, 397
PleI GAGTC 2 cut(s) 451, 577
PpsI GAGTC 2 cut(s) 451, 577
PspFI CCCAGC 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 554
PspPI GGNCC 1 cut(s) 893
PspXI VCTCGAGB 1 cut(s) 634
RsaI GTAC 5 cut(s) 13, 290, 683, 732, 956
RsaNI GTAC 5 cut(s) 12, 289, 682, 731, 955
RseI CAYNNNNRTG 2 cut(s) 738, 965
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 348, 363
SatI GCNGC 3 cut(s) 74, 309, 396
Sau96I GGNCC 1 cut(s) 893
ScaI AGTACT 2 cut(s) 13, 956
SchI GAGTC 2 cut(s) 451, 577
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 5 cut(s) 151, 392, 407, 601, 742
SfcI CTRYAG 1 cut(s) 726
Sfr274I CTCGAG 1 cut(s) 634
SinI GGWCC 1 cut(s) 893
SlaI CTCGAG 1 cut(s) 634
SmiMI CAYNNNNRTG 2 cut(s) 738, 965
SmlI CTYRAG 2 cut(s) 541, 634
SmoI CTYRAG 2 cut(s) 541, 634
SpeI ACTAGT 1 cut(s) 175
SphI GCATGC 1 cut(s) 224
Sse9I AATT 8 cut(s) 207, 225, 280, 686, 707, 720, 831, 906
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 2 cut(s) 39, 112
SspI AATATT 1 cut(s) 864
SspMI CTAG 2 cut(s) 15, 176
StuI AGGCCT 1 cut(s) 503
TaaI ACNGT 3 cut(s) 97, 361, 671
TaqI TCGA 2 cut(s) 635, 738
TasI AATT 8 cut(s) 207, 225, 280, 686, 707, 720, 831, 906
TatI WGTACW 4 cut(s) 11, 288, 730, 954
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 348, 363
Tru9I TTAA 2 cut(s) 348, 363
TscAI CASTG 5 cut(s) 100, 280, 411, 676, 1018
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 3 cut(s) 73, 308, 395
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 4 cut(s) 459, 462, 801, 810
TspGWI ACGGA 1 cut(s) 376
TspRI CASTG 5 cut(s) 100, 280, 411, 676, 1018
VpaK11BI GGWCC 1 cut(s) 893
XapI RAATTY 2 cut(s) 280, 720
XceI RCATGY 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 634
XspI CTAG 2 cut(s) 15, 176
ZrmI AGTACT 2 cut(s) 13, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.