RchiOBHm_Chr7g0195261

Anthocyanidin reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001687
Physical Location & Seq
Reverse (-)
13148015 .. 13150323
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17461

Sequence Viewer

Length: 1038 bp
ATGGAGAAGAACAAAGTCTCAAGAGTTTGTGTTACAGGTGGTTCCGGATATATTGGCTGTTGGCTAGTAAAAACGCTCCTAGAGAAGGGCCACACAGTCCATGCCACTCTCAGAAACTTGGAGGATGCATCAAAAGTAGGCCTTCTGAAGTCTCTCCCCAATGCAGACACCAATTTGTTGCTGTTCAAAGCTGATATATACGATCCTAATGAATTCGAGCCTGCAATTGAAGGGTGTGAGTTTGTTTTTCATGTTGCAACTCCCATGCAACATGATCCCCAAAACTCCCAGTACAAAGACACAGCTGAAGCTGCTGTTGCCGGGGTGAGAAGCATTGCTGATTCTTGCATTCGATCGCAGACAGTTAAGCGACTCATCTACACTGCAAGCATACTTTCTATGTCCCCAAGAAAGGATGATGGAGTTGGATTCAGACCCTTACTTGATGAGTCGTGTTGGACACCACTTGATGCCTCATCTACTTATGGCAATGCTATCACGATGGGGTACATTAAATCAAAGACCTTAGCTGAGAAAGCAGTGCTGAGCCACAATGACTTTGATGATGGTAAATTAGAAGTGGTAACTCTTCCTTGTGGCTTAGTGGGAGGAGAAAGTCTTCTTTCATATTTGCCTACAAGTGTGGGAGTGATTCTGGCACAACTTACTGGGGACTTGTTTTCTTGCAATGCCTTAAAATTAATGCAAGAATTTATGGGATCAGTTCCTCTGGTGCACATTGATGATGTTTGCCAAGCACATATCTTCTGCATGGAACAGCCATCAATGAAAGGCAGATTCTGTTGTGCAGTTGCCTGTCACTCTATCAAAGAAATTGCAATATACTTCCAAGAAACTTACCCAGAATACAAGATACAAAAAAAATTGACCGAAGGGCCTGAAGAAGGAAGCCAAAGTGATTTCAGTAAGCTGAAGAAGATGGGCTTTGAGTATAAGTATGGCATGAAGGACATACTAGATGACAGTGTAACATGGGCAAAGAAGTTAAATGGGAGCTCTCTTTCTCAAGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

345

Amino Acids

37.93

Weight (kDa)

5.67

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 109 2.2e-10 NmrA-like family
Epimerase PF01370 9 - 262 1.9e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 198 8e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 1.5e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 209 7.3e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 132 3.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 44
AclWI GGATC 3 cut(s) 197, 269, 727
AcsI RAATTY 2 cut(s) 212, 710
AcuI CTGAAG 4 cut(s) 167, 327, 921, 953
AfaI GTAC 2 cut(s) 293, 509
AfiI CCNNNNNNNGG 3 cut(s) 85, 412, 905
AgsI TTSAA 2 cut(s) 187, 230
AluBI AGCT 6 cut(s) 191, 305, 311, 530, 931, 1017
AluI AGCT 6 cut(s) 191, 305, 311, 530, 931, 1017
Alw21I GWGCWC 2 cut(s) 738, 1019
Alw26I GTCTC 2 cut(s) 22, 156
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 3 cut(s) 197, 269, 727
AlwNI CAGNNNCTG 1 cut(s) 801
Aor13HI TCCGGA 1 cut(s) 44
AoxI GGCC 3 cut(s) 88, 139, 896
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 212, 710
AseI ATTAAT 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 88, 896
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 738
BanII GRGCYC 1 cut(s) 1019
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 738, 1019
BbvI GCAGC 1 cut(s) 298
BccI CCATC 5 cut(s) 413, 496, 560, 790, 934
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 22, 156
BfaI CTAG 3 cut(s) 65, 80, 977
BisI GCNGC 1 cut(s) 312
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 88, 896
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 2 cut(s) 283, 678
BmsI GCATC 3 cut(s) 115, 137, 460
BmuI ACTGGG 2 cut(s) 283, 678
BpiI GAAGAC 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 2 cut(s) 4, 1011
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 412, 905
Bse1I ACTGG 2 cut(s) 289, 673
Bse3DI GCAATG 3 cut(s) 333, 496, 694
BseAI TCCGGA 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 2 cut(s) 130, 421
BseLI CCNNNNNNNGG 3 cut(s) 85, 412, 905
BseMI GCAATG 3 cut(s) 333, 496, 694
BseMII CTCAG 3 cut(s) 124, 522, 536
BseNI ACTGG 2 cut(s) 289, 673
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 90, 141, 898
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 2 cut(s) 738, 1019
BsiSI CCGG 2 cut(s) 45, 321
BslFI GGGAC 2 cut(s) 388, 686
BslI CCNNNNNNNGG 3 cut(s) 85, 412, 905
BsmAI GTCTC 2 cut(s) 22, 156
BsmFI GGGAC 2 cut(s) 388, 686
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 3 cut(s) 90, 141, 898
Bsp1286I GDGCHC 2 cut(s) 738, 1019
Bsp13I TCCGGA 1 cut(s) 44
Bsp143I GATC 4 cut(s) 202, 274, 353, 719
Bsp1720I GCTNAGC 1 cut(s) 545
BspANI GGCC 3 cut(s) 90, 141, 898
BspCNI CTCAG 3 cut(s) 123, 523, 537
BspEI TCCGGA 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 43
BspPI GGATC 3 cut(s) 197, 269, 727
BsrDI GCAATG 3 cut(s) 333, 496, 694
BsrI ACTGG 2 cut(s) 289, 673
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 202, 274, 353, 719
Bst4CI ACNGT 3 cut(s) 97, 364, 986
Bst6I CTCTTC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 222, 388
BstDEI CTNAG 5 cut(s) 110, 526, 531, 545, 601
BstENI CCTNNNNNAGG 2 cut(s) 83, 903
BstF5I GGATG 2 cut(s) 130, 421
BstKTI GATC 4 cut(s) 205, 277, 356, 722
BstMAI GTCTC 2 cut(s) 22, 156
BstMBI GATC 4 cut(s) 202, 274, 353, 719
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 3 cut(s) 311, 317, 536
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 738
BstV1I GCAGC 1 cut(s) 298
BstV2I GAAGAC 1 cut(s) 611
BsuRI GGCC 3 cut(s) 90, 141, 898
BtsCI GGATG 2 cut(s) 130, 421
BtsI GCAGTG 2 cut(s) 381, 546
BtsIMutI CAGTG 3 cut(s) 381, 546, 991
Cac8I GCNNGC 2 cut(s) 222, 388
CaiI CAGNNNCTG 1 cut(s) 801
Cfr13I GGNCC 2 cut(s) 88, 896
Csp6I GTAC 2 cut(s) 292, 508
CviAII CATG 7 cut(s) 101, 251, 265, 272, 772, 964, 993
CviQI GTAC 2 cut(s) 292, 508
DdeI CTNAG 5 cut(s) 110, 526, 531, 545, 601
DpnI GATC 4 cut(s) 204, 276, 355, 721
DpnII GATC 4 cut(s) 202, 274, 353, 719
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 1017
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 1019
Eco53kI GAGCTC 1 cut(s) 1017
Eco57I CTGAAG 4 cut(s) 167, 327, 921, 953
EcoICRI GAGCTC 1 cut(s) 1017
EcoNI CCTNNNNNAGG 2 cut(s) 83, 903
EcoO109I RGGNCCY 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 212
EcoT22I ATGCAT 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 1019
FaeI CATG 7 cut(s) 104, 254, 268, 275, 775, 967, 996
FalI AAGNNNNNCTT 4 cut(s) 126, 158, 929, 961
FaqI GGGAC 2 cut(s) 388, 686
FatI CATG 7 cut(s) 100, 250, 264, 271, 771, 963, 992
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 137, 428
FriOI GRGCYC 1 cut(s) 1019
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 3 cut(s) 65, 80, 977
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 3 cut(s) 90, 141, 898
HapII CCGG 2 cut(s) 45, 321
Hin1II CATG 7 cut(s) 104, 254, 268, 275, 775, 967, 996
HinfI GANTC 6 cut(s) 341, 372, 429, 449, 652, 798
HpaII CCGG 2 cut(s) 45, 321
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 3 cut(s) 113, 147, 434
Hpy188III TCNNGA 3 cut(s) 21, 45, 499
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 6 cut(s) 79, 152, 224, 887, 899, 961
HpyCH4III ACNGT 3 cut(s) 97, 364, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 317, 536
HpyF3I CTNAG 5 cut(s) 110, 526, 531, 545, 601
Hsp92II CATG 7 cut(s) 104, 254, 268, 275, 775, 967, 996
Kpn2I TCCGGA 1 cut(s) 44
Kzo9I GATC 4 cut(s) 202, 274, 353, 719
LmnI GCTCC 2 cut(s) 81, 1014
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 3 cut(s) 115, 137, 460
MaeI CTAG 3 cut(s) 65, 80, 977
MaeIII GTNAC 4 cut(s) 31, 583, 818, 988
MalI GATC 4 cut(s) 204, 276, 355, 721
MboI GATC 4 cut(s) 202, 274, 353, 719
MboII GAAGA 7 cut(s) 19, 581, 611, 757, 914, 946, 949
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 738, 1019
MluCI AATT 8 cut(s) 172, 212, 225, 572, 698, 710, 834, 884
MlyI GAGTC 2 cut(s) 366, 458
MmeI TCCRAC 2 cut(s) 406, 437
MnlI CCTC 4 cut(s) 115, 484, 602, 738
Mph1103I ATGCAT 1 cut(s) 130
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 618
MseI TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
MslI CAYNNNNRTG 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 2 cut(s) 45, 321
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 311, 317, 536
NciI CCSGG 1 cut(s) 322
NdeII GATC 4 cut(s) 202, 274, 353, 719
NlaIII CATG 7 cut(s) 104, 254, 268, 275, 775, 967, 996
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 130
PceI AGGCCT 1 cut(s) 141
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 341, 429, 652, 798
PkrI GCNGC 1 cut(s) 313
Ple19I CGATCG 1 cut(s) 356
PleI GAGTC 2 cut(s) 366, 457
PpsI GAGTC 2 cut(s) 366, 457
PshBI ATTAAT 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 1019
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 2 cut(s) 88, 896
PstNI CAGNNNCTG 1 cut(s) 801
PvuI CGATCG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 293, 509
RsaNI GTAC 2 cut(s) 292, 508
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 1 cut(s) 1019
SaqAI TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 202, 274, 353, 719
Sau96I GGNCC 2 cut(s) 88, 896
SchI GAGTC 2 cut(s) 366, 458
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 738, 1019
SetI ASST 8 cut(s) 40, 193, 307, 313, 527, 532, 933, 1019
SfaNI GCATC 3 cut(s) 115, 137, 460
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 2 cut(s) 19, 1026
SmoI CTYRAG 2 cut(s) 19, 1026
Sse9I AATT 8 cut(s) 172, 212, 225, 572, 698, 710, 834, 884
SseBI AGGCCT 1 cut(s) 141
SspMI CTAG 3 cut(s) 65, 80, 977
SstI GAGCTC 1 cut(s) 1019
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 97, 364, 986
TaqI TCGA 2 cut(s) 216, 352
TaqII GACCGA 1 cut(s) 905
TasI AATT 8 cut(s) 172, 212, 225, 572, 698, 710, 834, 884
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 4 cut(s) 341, 429, 652, 798
Tru1I TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
Tru9I TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
TscAI CASTG 3 cut(s) 388, 546, 991
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 5 cut(s) 225, 239, 615, 803, 980
TspRI CASTG 3 cut(s) 388, 546, 991
VneI GTGCAC 1 cut(s) 734
VspI ATTAAT 1 cut(s) 701
XagI CCTNNNNNAGG 2 cut(s) 83, 903
XapI RAATTY 2 cut(s) 212, 710
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 3 cut(s) 65, 80, 977
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.